Serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 892 |
| Acc36I | ACCTGC | 2 cut(s) | 823, 892 |
| AccB1I | GGYRCC | 1 cut(s) | 599 |
| AccIII | TCCGGA | 1 cut(s) | 352 |
| AciI | CCGC | 4 cut(s) | 34, 37, 48, 407 |
| AcsI | RAATTY | 5 cut(s) | 252, 583, 589, 613, 904 |
| AcyI | GRCGYC | 1 cut(s) | 824 |
| AfaI | GTAC | 2 cut(s) | 607, 913 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 154, 155, 457 |
| AflIII | ACRYGT | 1 cut(s) | 837 |
| AgsI | TTSAA | 7 cut(s) | 476, 481, 594, 612, 794, 894, 951 |
| AjnI | CCWGG | 1 cut(s) | 207 |
| AluBI | AGCT | 5 cut(s) | 349, 413, 806, 857, 983 |
| AluI | AGCT | 5 cut(s) | 349, 413, 806, 857, 983 |
| Alw26I | GTCTC | 1 cut(s) | 326 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 416 |
| Aor13HI | TCCGGA | 1 cut(s) | 352 |
| AoxI | GGCC | 2 cut(s) | 56, 522 |
| ApeKI | GCWGC | 5 cut(s) | 102, 410, 413, 803, 857 |
| ApoI | RAATTY | 5 cut(s) | 252, 583, 589, 613, 904 |
| AspLEI | GCGC | 3 cut(s) | 80, 372, 424 |
| AspS9I | GGNCC | 3 cut(s) | 6, 685, 920 |
| AsuHPI | GGTGA | 1 cut(s) | 727 |
| AvaII | GGWCC | 3 cut(s) | 6, 685, 920 |
| AxyI | CCTNAGG | 1 cut(s) | 526 |
| BanI | GGYRCC | 1 cut(s) | 599 |
| BbvI | GCAGC | 5 cut(s) | 114, 400, 422, 815, 844 |
| BccI | CCATC | 1 cut(s) | 458 |
| BciT130I | CCWGG | 1 cut(s) | 209 |
| BclI | TGATCA | 1 cut(s) | 225 |
| BcoDI | GTCTC | 1 cut(s) | 326 |
| BfmI | CTRYAG | 2 cut(s) | 100, 801 |
| BfuAI | ACCTGC | 2 cut(s) | 823, 892 |
| BglII | AGATCT | 1 cut(s) | 641 |
| BisI | GCNGC | 8 cut(s) | 35, 38, 103, 408, 411, 414, 804, 858 |
| BlpI | GCTNAGC | 1 cut(s) | 345 |
| BlsI | GCNGC | 8 cut(s) | 36, 39, 104, 409, 412, 415, 805, 859 |
| BmcAI | AGTACT | 1 cut(s) | 913 |
| Bme1390I | CCNGG | 1 cut(s) | 209 |
| Bme18I | GGWCC | 3 cut(s) | 6, 685, 920 |
| BmgT120I | GGNCC | 3 cut(s) | 6, 685, 920 |
| BmiI | GGNNCC | 4 cut(s) | 7, 396, 601, 686 |
| BmrFI | CCNGG | 1 cut(s) | 209 |
| BmrI | ACTGGG | 1 cut(s) | 675 |
| BmsI | GCATC | 5 cut(s) | 76, 346, 368, 409, 712 |
| BmuI | ACTGGG | 1 cut(s) | 675 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 345 |
| BsaHI | GRCGYC | 1 cut(s) | 824 |
| BsaWI | WCCGGW | 3 cut(s) | 352, 397, 620 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 271, 301 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 154, 155, 457 |
| Bse1I | ACTGG | 2 cut(s) | 505, 670 |
| Bse21I | CCTNAGG | 1 cut(s) | 526 |
| Bse3DI | GCAATG | 1 cut(s) | 825 |
| BseAI | TCCGGA | 1 cut(s) | 352 |
| BseBI | CCWGG | 1 cut(s) | 209 |
| BseGI | GGATG | 2 cut(s) | 361, 617 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 154, 155, 457 |
| BseMI | GCAATG | 1 cut(s) | 825 |
| BseMII | CTCAG | 3 cut(s) | 55, 540, 575 |
| BseNI | ACTGG | 2 cut(s) | 505, 670 |
| BseRI | GAGGAG | 1 cut(s) | 546 |
| BseXI | GCAGC | 5 cut(s) | 114, 400, 422, 815, 844 |
| BsgI | GTGCAG | 1 cut(s) | 506 |
| BshFI | GGCC | 2 cut(s) | 58, 524 |
| BshNI | GGYRCC | 1 cut(s) | 599 |
| BsiSI | CCGG | 3 cut(s) | 353, 398, 621 |
| BslFI | GGGAC | 2 cut(s) | 203, 698 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 154, 155, 457 |
| BsmAI | GTCTC | 1 cut(s) | 326 |
| BsmBI | CGTCTC | 1 cut(s) | 326 |
| BsmFI | GGGAC | 2 cut(s) | 203, 698 |
| BsmI | GAATGC | 1 cut(s) | 302 |
| BsnI | GGCC | 2 cut(s) | 58, 524 |
| Bsp13I | TCCGGA | 1 cut(s) | 352 |
| Bsp143I | GATC | 3 cut(s) | 225, 641, 808 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 345 |
| BspACI | CCGC | 4 cut(s) | 34, 37, 48, 407 |
| BspANI | GGCC | 2 cut(s) | 58, 524 |
| BspCNI | CTCAG | 3 cut(s) | 54, 539, 574 |
| BspEI | TCCGGA | 1 cut(s) | 352 |
| BspHI | TCATGA | 1 cut(s) | 751 |
| BspLI | GGNNCC | 4 cut(s) | 7, 396, 601, 686 |
| BspMAI | CTGCAG | 2 cut(s) | 104, 805 |
| BspMI | ACCTGC | 2 cut(s) | 823, 892 |
| BspT107I | GGYRCC | 1 cut(s) | 599 |
| BsrDI | GCAATG | 1 cut(s) | 825 |
| BsrI | ACTGG | 2 cut(s) | 505, 670 |
| BssMI | GATC | 3 cut(s) | 225, 641, 808 |
| BssNI | GRCGYC | 1 cut(s) | 824 |
| Bst2UI | CCWGG | 1 cut(s) | 209 |
| Bst4CI | ACNGT | 4 cut(s) | 179, 310, 316, 515 |
| Bst6I | CTCTTC | 1 cut(s) | 870 |
| BstACI | GRCGYC | 1 cut(s) | 824 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 516 |
| BstC8I | GCNNGC | 3 cut(s) | 32, 56, 233 |
| BstDEI | CTNAG | 6 cut(s) | 41, 68, 345, 526, 561, 638 |
| BstF5I | GGATG | 2 cut(s) | 361, 617 |
| BstHHI | GCGC | 3 cut(s) | 80, 372, 424 |
| BstKTI | GATC | 3 cut(s) | 228, 644, 811 |
| BstMAI | GTCTC | 1 cut(s) | 326 |
| BstMBI | GATC | 3 cut(s) | 225, 641, 808 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 86, 413, 419, 516, 755 |
| BstNI | CCWGG | 1 cut(s) | 209 |
| BstNSI | RCATGY | 4 cut(s) | 120, 207, 235, 841 |
| BstSCI | CCNGG | 1 cut(s) | 207 |
| BstSFI | CTRYAG | 2 cut(s) | 100, 801 |
| BstV1I | GCAGC | 5 cut(s) | 114, 400, 422, 815, 844 |
| BstX2I | RGATCY | 1 cut(s) | 641 |
| BstYI | RGATCY | 1 cut(s) | 641 |
| Bsu36I | CCTNAGG | 1 cut(s) | 526 |
| BsuRI | GGCC | 2 cut(s) | 58, 524 |
| BtsCI | GGATG | 2 cut(s) | 361, 617 |
| BtsIMutI | CAGTG | 2 cut(s) | 291, 663 |
| BveI | ACCTGC | 2 cut(s) | 823, 892 |
| Cac8I | GCNNGC | 3 cut(s) | 32, 56, 233 |
| CaiI | CAGNNNCTG | 1 cut(s) | 416 |
| CciI | TCATGA | 1 cut(s) | 751 |
| CfoI | GCGC | 3 cut(s) | 80, 372, 424 |
| Cfr13I | GGNCC | 3 cut(s) | 6, 685, 920 |
| CseI | GACGC | 3 cut(s) | 343, 714, 813 |
| Csp6I | GTAC | 2 cut(s) | 606, 912 |
| CviAII | CATG | 8 cut(s) | 117, 204, 232, 262, 367, 659, 752, 838 |
| CviQI | GTAC | 2 cut(s) | 606, 912 |
| DdeI | CTNAG | 6 cut(s) | 41, 68, 345, 526, 561, 638 |
| DpnI | GATC | 3 cut(s) | 227, 643, 810 |
| DpnII | GATC | 3 cut(s) | 225, 641, 808 |
| DraI | TTTAAA | 1 cut(s) | 588 |
| Eam1104I | CTCTTC | 1 cut(s) | 870 |
| EarI | CTCTTC | 1 cut(s) | 870 |
| Eco47I | GGWCC | 3 cut(s) | 6, 685, 920 |
| Eco81I | CCTNAGG | 1 cut(s) | 526 |
| EcoO109I | RGGNCCY | 1 cut(s) | 685 |
| EcoRI | GAATTC | 1 cut(s) | 252 |
| EcoRII | CCWGG | 1 cut(s) | 207 |
| EcoT22I | ATGCAT | 2 cut(s) | 91, 361 |
| Esp3I | CGTCTC | 1 cut(s) | 326 |
| FaeI | CATG | 8 cut(s) | 120, 207, 235, 265, 370, 662, 755, 841 |
| FaqI | GGGAC | 2 cut(s) | 203, 698 |
| FatI | CATG | 8 cut(s) | 116, 203, 231, 261, 366, 658, 751, 837 |
| FauNDI | CATATG | 1 cut(s) | 91 |
| FbaI | TGATCA | 1 cut(s) | 225 |
| Fnu4HI | GCNGC | 8 cut(s) | 35, 38, 103, 408, 411, 414, 804, 858 |
| FokI | GGATG | 2 cut(s) | 368, 604 |
| Fsp4HI | GCNGC | 8 cut(s) | 35, 38, 103, 408, 411, 414, 804, 858 |
| GlaI | GCGC | 3 cut(s) | 79, 371, 423 |
| GluI | GCNGC | 8 cut(s) | 35, 38, 103, 408, 411, 414, 804, 858 |
| HaeIII | GGCC | 2 cut(s) | 58, 524 |
| HapII | CCGG | 3 cut(s) | 353, 398, 621 |
| HgaI | GACGC | 3 cut(s) | 343, 714, 813 |
| HhaI | GCGC | 3 cut(s) | 80, 372, 424 |
| Hin1I | GRCGYC | 1 cut(s) | 824 |
| Hin1II | CATG | 8 cut(s) | 120, 207, 235, 265, 370, 662, 755, 841 |
| Hin6I | GCGC | 3 cut(s) | 78, 370, 422 |
| HinP1I | GCGC | 3 cut(s) | 78, 370, 422 |
| HincII | GTYRAC | 1 cut(s) | 380 |
| HindII | GTYRAC | 1 cut(s) | 380 |
| HindIII | AAGCTT | 1 cut(s) | 347 |
| HinfI | GANTC | 1 cut(s) | 797 |
| HpaII | CCGG | 3 cut(s) | 353, 398, 621 |
| HphI | GGTGA | 1 cut(s) | 727 |
| Hpy166II | GTNNAC | 1 cut(s) | 380 |
| Hpy188I | TCNGA | 4 cut(s) | 23, 44, 145, 251 |
| Hpy188III | TCNNGA | 7 cut(s) | 220, 257, 353, 451, 528, 752, 794 |
| Hpy8I | GTNNAC | 1 cut(s) | 380 |
| HpyAV | CCTTC | 4 cut(s) | 670, 692, 696, 933 |
| HpyCH4III | ACNGT | 4 cut(s) | 179, 310, 316, 515 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 86, 413, 419, 516, 755 |
| HpyF3I | CTNAG | 6 cut(s) | 41, 68, 345, 526, 561, 638 |
| Hsp92I | GRCGYC | 1 cut(s) | 824 |
| Hsp92II | CATG | 8 cut(s) | 120, 207, 235, 265, 370, 662, 755, 841 |
| HspAI | GCGC | 3 cut(s) | 78, 370, 422 |
| Kpn2I | TCCGGA | 1 cut(s) | 352 |
| Ksp22I | TGATCA | 1 cut(s) | 225 |
| Kzo9I | GATC | 3 cut(s) | 225, 641, 808 |
| LmnI | GCTCC | 2 cut(s) | 158, 377 |
| Lsp1109I | GCAGC | 5 cut(s) | 114, 400, 422, 815, 844 |
| LweI | GCATC | 5 cut(s) | 76, 346, 368, 409, 712 |
| MaeIII | GTNAC | 2 cut(s) | 199, 310 |
| MalI | GATC | 3 cut(s) | 227, 643, 810 |
| MboI | GATC | 3 cut(s) | 225, 641, 808 |
| MboII | GAAGA | 2 cut(s) | 857, 918 |
| MflI | RGATCY | 1 cut(s) | 641 |
| MnlI | CCTC | 8 cut(s) | 38, 190, 451, 524, 535, 706, 748, 871 |
| Mph1103I | ATGCAT | 2 cut(s) | 91, 361 |
| MroI | TCCGGA | 1 cut(s) | 352 |
| MseI | TTAA | 2 cut(s) | 587, 761 |
| MspA1I | CMGCKG | 2 cut(s) | 413, 806 |
| MspI | CCGG | 3 cut(s) | 353, 398, 621 |
| MspR9I | CCNGG | 1 cut(s) | 209 |
| Mva1269I | GAATGC | 1 cut(s) | 302 |
| MvaI | CCWGG | 1 cut(s) | 209 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 86, 413, 419, 516, 755 |
| NdeI | CATATG | 1 cut(s) | 91 |
| NdeII | GATC | 3 cut(s) | 225, 641, 808 |
| NlaIII | CATG | 8 cut(s) | 120, 207, 235, 265, 370, 662, 755, 841 |
| NlaIV | GGNNCC | 4 cut(s) | 7, 396, 601, 686 |
| NmuCI | GTSAC | 2 cut(s) | 199, 310 |
| NsiI | ATGCAT | 2 cut(s) | 91, 361 |
| NspI | RCATGY | 4 cut(s) | 120, 207, 235, 841 |
| PaeI | GCATGC | 1 cut(s) | 235 |
| PagI | TCATGA | 1 cut(s) | 751 |
| PaqCI | CACCTGC | 1 cut(s) | 892 |
| PciI | ACATGT | 1 cut(s) | 837 |
| PctI | GAATGC | 1 cut(s) | 302 |
| PfeI | GAWTC | 1 cut(s) | 797 |
| PflFI | GACNNNGTC | 1 cut(s) | 215 |
| PkrI | GCNGC | 8 cut(s) | 36, 39, 104, 409, 412, 415, 805, 859 |
| PpuMI | RGGWCCY | 1 cut(s) | 685 |
| PscI | ACATGT | 1 cut(s) | 837 |
| Psp5II | RGGWCCY | 1 cut(s) | 685 |
| Psp6I | CCWGG | 1 cut(s) | 207 |
| PspGI | CCWGG | 1 cut(s) | 207 |
| PspN4I | GGNNCC | 4 cut(s) | 7, 396, 601, 686 |
| PspPI | GGNCC | 3 cut(s) | 6, 685, 920 |
| PspPPI | RGGWCCY | 1 cut(s) | 685 |
| PstI | CTGCAG | 2 cut(s) | 104, 805 |
| PstNI | CAGNNNCTG | 1 cut(s) | 416 |
| PsuI | RGATCY | 1 cut(s) | 641 |
| PsyI | GACNNNGTC | 1 cut(s) | 215 |
| PvuII | CAGCTG | 2 cut(s) | 413, 806 |
| RsaI | GTAC | 2 cut(s) | 607, 913 |
| RsaNI | GTAC | 2 cut(s) | 606, 912 |
| SaqAI | TTAA | 2 cut(s) | 587, 761 |
| SatI | GCNGC | 8 cut(s) | 35, 38, 103, 408, 411, 414, 804, 858 |
| Sau3AI | GATC | 3 cut(s) | 225, 641, 808 |
| Sau96I | GGNCC | 3 cut(s) | 6, 685, 920 |
| ScaI | AGTACT | 1 cut(s) | 913 |
| ScrFI | CCNGG | 1 cut(s) | 209 |
| SfaNI | GCATC | 5 cut(s) | 76, 346, 368, 409, 712 |
| SfcI | CTRYAG | 2 cut(s) | 100, 801 |
| SinI | GGWCC | 3 cut(s) | 6, 685, 920 |
| SmiI | ATTTAAAT | 1 cut(s) | 588 |
| SphI | GCATGC | 1 cut(s) | 235 |
| SsiI | CCGC | 4 cut(s) | 34, 37, 48, 407 |
| StyD4I | CCNGG | 1 cut(s) | 207 |
| SwaI | ATTTAAAT | 1 cut(s) | 588 |
| TaaI | ACNGT | 4 cut(s) | 179, 310, 316, 515 |
| TaqI | TCGA | 3 cut(s) | 195, 811, 985 |
| TaqII | GACCGA | 1 cut(s) | 281 |
| TatI | WGTACW | 2 cut(s) | 605, 911 |
| TauI | GCSGC | 3 cut(s) | 37, 40, 410 |
| TfiI | GAWTC | 1 cut(s) | 797 |
| Tru1I | TTAA | 2 cut(s) | 587, 761 |
| Tru9I | TTAA | 2 cut(s) | 587, 761 |
| TscAI | CASTG | 2 cut(s) | 298, 670 |
| TseFI | GTSAC | 2 cut(s) | 199, 310 |
| TseI | GCWGC | 5 cut(s) | 102, 410, 413, 803, 857 |
| Tsp45I | GTSAC | 2 cut(s) | 199, 310 |
| TspDTI | ATGAA | 4 cut(s) | 556, 606, 615, 867 |
| TspGWI | ACGGA | 1 cut(s) | 29 |
| TspRI | CASTG | 2 cut(s) | 298, 670 |
| Tth111I | GACNNNGTC | 1 cut(s) | 215 |
| VpaK11BI | GGWCC | 3 cut(s) | 6, 685, 920 |
| XapI | RAATTY | 5 cut(s) | 252, 583, 589, 613, 904 |
| XceI | RCATGY | 4 cut(s) | 120, 207, 235, 841 |
| ZrmI | AGTACT | 1 cut(s) | 913 |
| Zsp2I | ATGCAT | 2 cut(s) | 91, 361 |