MD17G1088600.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000114
Physical Location & Seq
Forward (+)
7279597 .. 7282778
3182 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1088600.v1.1.491

Sequence Viewer

Length: 2472 bp
ATGTCGCTGCTCATTGCTGGTCTTCTGAAATTAATAGTGGTGTTGTCTCTCATGCTGCTGCCATTTTCTTTTTGTACGGCAAGCAAACACAACAACTTATTAGTACCAGGCCATTCTATCATTGCAAGACAAACTTTGATTTCTTCCTCCGGAAACTTTGCACTTGGTTTCTTCCGTCCTGCAAAGTCCACCAAATATTTCCTTGGTATTTGGTACAATGCAATGCCAAACCCTCCAATTGTGTGGGTTGCAAACAGAGACTCTCCGCTCAATTCTCCGGGGGTCCTCATGCTGAGAAGTGATGGAAATTTGGTTGTGCTGGAAAATGAAGCCATCATCTGGTCATCAAATGCATCAGTTCCAGCTTCTGCAATGAGTTCGACAATTGGGTTGCTAATGGATTCCGGAAACTTTGTTCTCCAATTAGGAGAAGAGTTGGATAAAAACCCTTTGTGGCAGAGCTTTGATCATCCCTCAGATACATTGCTGCCCGGCATGAAGATTAGCCTCAACAAAAGGACAGGCCAGCAAAGGCGCCTTACATCCTGGTCCGCAGTTGATGATCCAAAACCCGGGAATTTCTCTGCTGGCATTTATCCCAAAGTGCCTGCCCAGATTGTAGTCTGGAAGGAGAACATTGGTGATCATCCCTACTGGAGAACTGCTGTCTACAATATGAACGGCCACAAGTCAAAATCAATTGTCATTAGAAATCTCAGTGGAACTTTCTTGTTCCTTTCTTACAATTTTGATCTTCATGATCATCATGACAATGATAATGATGAGATTTATTTTAATTATAGCTCCCCGGATATTAGCTCGGTGAAAATGAGGTCTGTGTTGGACCCGAATGGGCTGCTTGTCCTGCTGCTGTGGCAGGATGAGAGTGAGAAATGGAGTGAACTGTGGAGGGAGCCAGTCGATAAATGCGATTTTTATGCTCCTTGTGGTCCTTATGCTGCTTGTACTAGAAATGAAACCGTGTCACCATGTAAGTGTGTGACCGGTTTTGTACCTAAATTTGAACAACAATGGGATATGGGGGATTGGACTGGTGGGTGTGTTAGGGAGAAGGGGTTAAAATGCAATCGAAACAGAGAAGGGTTTTCCAAGTTGGAGAAGCTGAAACTACCGGATCATGCTGTTCTGGTTGGAGATAAAAAGAGTAGGACTGAGTGTGAAGCTGAATGCCTTCAAAACCGCTCTTGCACAGCTTATGTTTACGCAAATGTGGCACAGGAAATTAGTTCTATGAGATGTATAACATGGTACGGTGACTTGGTGGATCTTGTTGAGAATCACACTCTCGGGCAAGACATTTATATTCGTGTTCATGAGGATGACTGCAATGATTGTGACGGTTGTAAGGGTCATGTTGAAATTTTCTTGAAGCAACGATCAGATGTAATTGCCATTGCAATTGTCTCAGCAATCGTAGGAATTCTTGCAACCATTTTTGGATATCTATTATGGAAGAAAACTGTGAGAAATGAAGGGAAAGCTGGAGGTGGAAGGAACGATACTGATCAACTGCCACCTTTCAGCTTAAAGAGTATATTAGCTGCTACAAACAACTTCTCTGATACTAATAAAATCGGAGAGGGAGGATTTGGCCCTGTTTATAAGGGGACTTTGCCTGAAAATCAAGAAGTAGCCATTAAAAGGCTGTCAAAGAAGTCAGGGCAAGGACATCGAGAATTCATAAATGAGTTAGAGCTTATAGCCAAGCTCCAACATACCAATCTTGTTAGGCTGTTGGGTTTCTGCAATGAAGAGGAGGAAATGATATTGATCTACGAGTACCTGCCGAATCGAAGTTTGGACAAATTTTTGTTCGATCCATCTGAAAAGACAAAATTGGATTGGGGGAAACGCTTTCAAATCATAGAAGGTATTGCTCAAGGAGTACTTTATATCCACAAGTACTCCAGATTGAAAATCATCCATAGGGACCTCAAAGCAAGTAATATACTGTTGGATGAAGCAATGAACCCTAAAATTTCAGACTTTGGAATGGCGAAGATTTTCAGGATGAATCAAATCGAAGCAAATACCAACAGGATTGTTGGAACATACGGCTACATGTCCCCTGAGTATGCATTCTTTGGCCAATTCTCTGAGAAATTGGATGTATTCAGCTTTGGAGTGTTGTTGTTAGAGATTGCATGGGAATTATGCAAAGAAGGTAGAGGAATGGAGGTGATTGATGAATCAGTAAGGGAAACATGCTGGTCGGATGAAGCTCTAAGGTGCCTCCATGTAGGGTTTTTGTGTGTTCAAGAAGCTCCAGATGATCGACCAACAATGTCTTCGGTAATTTGCATGCTGACTAATGAAGCTACATCACTTCCAGCCTCCAAAGAACCTGCTTTCACAACACATAAGAATTCCATTGCTGCTGGTTCTTATCCTCGAGCATCTACCAGCTTTTCCAACAATGCAATCACCATTAGTTTGATAGTAGGTAGATAG

Protein Analysis

824

Amino Acids

92.26

Weight (kDa)

6.11

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 80 - 183 5e-31 D-mannose binding lectin
S_locus_glycop PF00954 253 - 341 6.7e-19 S-locus glycoprotein domain
PAN_2 PF08276 362 - 430 2e-14 PAN-like domain
Pkinase PF00069 527 - 722 3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 530 - 774 4.4e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 778 - 823 7.5e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000114)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_4g01160 FvH4_6g34940 FvH4_6g44062 FvH4_6g44106 FvH4_6g44106 FvH4_6g44106 FvH4_6g44107 FvH4_6g44107 FvH4_6g44109 FvH4_6g44140 FvH4_6g44190 FvH4_6g44200 FvH4_6g44240 FvH4_6g44241 FvH4_6g44242
malus_domestica MD09G1094900.v1.1 MD09G1100300.v1.1 MD09G1100400.v1.1 MD09G1100600.v1.1 MD09G1100800.v1.1 MD09G1100900.v1.1 MD09G1101300.v1.1 MD09G1255200.v1.1 MD17G1088400.v1.1 MD17G1088600.v1.1 MD17G1247900.v1.1 MD17G1248300.v1.1
prunus_persica Prupe.1G497000_v2.0.a1 Prupe.2G033300_v2.0.a1 Prupe.3G145500_v2.0.a1 Prupe.3G225400_v2.0.a1 Prupe.3G225700_v2.0.a1 Prupe.3G225700_v2.0.a1
pyrus_communis pycom06g12720 pycom06g12740 pycom09g01960 pycom09g02450 pycom17g08520 pycom17g08530 pycom17g08560 pycom17g24950 pycom17g25030 pycom17g25110 pycom17g25260 pycom17g25290
rosa_chinensis RchiOBHm_Chr1g0326521 RchiOBHm_Chr1g0326551 RchiOBHm_Chr1g0326591 RchiOBHm_Chr1g0328741 RchiOBHm_Chr1g0329371 RchiOBHm_Chr2g0145911 RchiOBHm_Chr2g0160891 RchiOBHm_Chr2g0160921 RchiOBHm_Chr2g0161001 RchiOBHm_Chr2g0161011 RchiOBHm_Chr2g0161431 RchiOBHm_Chr2g0165121 RchiOBHm_Chr2g0166301 RchiOBHm_Chr4g0399551 RchiOBHm_Chr4g0405681 RchiOBHm_Chr5g0066741 RchiOBHm_Chr5g0066841 RchiOBHm_Chr6g0273021 RchiOBHm_Chr7g0225151
rosa_laevigata RLG00000001843 RLG00000008827 RLG00000014283 RLG00000014284 RLG00000020173 RLG00000021244 RLG00000021246 RLG00000021248 RLG00000021283 RLG00000023606 RLG00000029921 RLG00000030066 RLG00000030067 RLG00000033456
rosa_multiflora Rmu_co7958161.1_g000001 Rmu_co8440151.1_g000001 Rmu_co8454989.1_g000001 Rmu_sc0000129.1_g000018 Rmu_sc0000322.1_g000011 Rmu_sc0000322.1_g000045 Rmu_sc0000605.1_g000051 Rmu_sc0000955.1_g000015 Rmu_sc0000955.1_g000020 Rmu_sc0001188.1_g000017 Rmu_sc0001188.1_g000019 Rmu_sc0001355.1_g000015 Rmu_sc0001702.1_g000005 Rmu_sc0001702.1_g000006 Rmu_sc0001702.1_g000007 Rmu_sc0001702.1_g000008 Rmu_sc0001702.1_g000009 Rmu_sc0001702.1_g000010 Rmu_sc0001702.1_g000011 Rmu_sc0001702.1_g000012 Rmu_sc0001702.1_g000013 Rmu_sc0001702.1_g000014 Rmu_sc0001702.1_g000015 Rmu_sc0001702.1_g000016 Rmu_sc0002473.1_g000012 Rmu_sc0002473.1_g000015 Rmu_sc0002773.1_g000029 Rmu_sc0004178.1_g000002 Rmu_sc0005189.1_g000006 Rmu_sc0005860.1_g000004 Rmu_sc0009205.1_g000005 Rmu_sc0009205.1_g000007 Rmu_sc0009205.1_g000023 Rmu_sc0009472.1_g000005 Rmu_sc0009858.1_g000015 Rmu_sc0020187.1_g000001
rosa_roxburghii Rroxscaffold_176G00431460 Rroxscaffold_176G00431480 Rroxscaffold_1G00014330 Rroxscaffold_1G00031590 Rroxscaffold_2G00088820 Rroxscaffold_2G00089330 Rroxscaffold_2G00089360 Rroxscaffold_2G00089370 Rroxscaffold_2G00089380 Rroxscaffold_2G00100370 Rroxscaffold_2G00100380 Rroxscaffold_4G00321410 Rroxscaffold_4G00323700 Rroxscaffold_4G00327640 Rroxscaffold_4G00327650 Rroxscaffold_5G00344450 Rroxscaffold_7G00202770 Rroxscaffold_7G00203450 Rroxscaffold_7G00203540
rosa_rugosa Rorug01G0061100 Rorug02G0482100 Rorug02G0485900 Rorug02G0485900 Rorug02G0489500 Rorug02G0489600 Rorug02G0489700 Rorug04G0016600 Rorug04G0016700 Rorug05G0379000 Rorug05G0516700 Rorug06G0007900 Rorug06G0009300
rosa_samantha Rh1AG076700 Rh1AG076800 Rh1AG077000 Rh1AG077300 Rh1AG094400 Rh1AG097500 Rh1AG402000 Rh1BG062700 Rh1BG062900 Rh1CG075500 Rh1CG075600 Rh1CG075800 Rh1CG090900 Rh1CG091400 Rh1CG091600 Rh1CG094100 Rh1DG082300 Rh1DG082500 Rh1DG097400 Rh2AG448200 Rh2AG448300 Rh2AG511600 Rh2AG547500 Rh2AG551400 Rh2AG551700 Rh2AG555800 Rh2AG588000 Rh2BG565900 Rh2BG566000 Rh2BG566300 Rh2BG569000 Rh2BG600000 Rh2CG435200 Rh2CG435300 Rh2CG496600 Rh2CG535400 Rh2CG535600 Rh2CG539700 Rh2CG562700 Rh2CG570900 Rh2DG574600 Rh2DG574800 Rh2DG578600 Rh2DG610200 Rh4CG140900 Rh4DG127800 Rh5AG438500 Rh5BG436500 Rh5BG455500 Rh5CG477600 Rh5DG470300 Rh6AG129500 Rh6AG129900 Rh6BG125700 Rh6BG126000 Rh6BG126700 Rh6BG126800 Rh6CG124500 Rh6CG124800 Rh6CG125200 Rh6CG125300 Rh6DG111400 Rh6DG111700 Rh6DG111800 Rh7DG373300
rosa_wichuraiana Rw0G014010 Rw1G006140 Rw1G006160 Rw1G006180 Rw1G006190 Rw1G007340 Rw1G007360 Rw2G045660 Rw2G045670 Rw2G045970 Rw2G048220 Rw4G010820 Rw5G041010 Rw6G011220 Rw6G011230 Rw7G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1623
Acc36I ACCTGC 2 cut(s) 1812, 2374
AccB1I GGYRCC 2 cut(s) 534, 2250
AccB7I CCANNNNNTGG 1 cut(s) 339
AccBSI CCGCTC 2 cut(s) 268, 1203
AccI GTMKAC 1 cut(s) 669
AccIII TCCGGA 2 cut(s) 149, 404
AciI CCGC 3 cut(s) 266, 552, 1201
AclWI GGATC 4 cut(s) 557, 1143, 1293, 1832
AcoI YGGCCR 2 cut(s) 682, 2107
AcsI RAATTY 9 cut(s) 307, 577, 1019, 1380, 1440, 1697, 1826, 1998, 2386
AcyI GRCGYC 1 cut(s) 535
AfaI GTAC 9 cut(s) 76, 105, 215, 967, 1014, 1271, 1802, 1908, 1925
AfiI CCNNNNNNNGG 3 cut(s) 339, 572, 1662
AflIII ACRYGT 1 cut(s) 2082
AgeI ACCGGT 1 cut(s) 1004
AgsI TTSAA 7 cut(s) 1025, 1196, 1379, 1390, 1880, 1936, 2279
AjnI CCWGG 2 cut(s) 106, 545
AjuI GAANNNNNNNTTGG 4 cut(s) 1802, 1834, 2293, 2325
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
Alw26I GTCTC 3 cut(s) 51, 252, 1429
AlwI GGATC 4 cut(s) 557, 1143, 1293, 1832
AlwNI CAGNNNCTG 1 cut(s) 368
Ama87I CYCGRG 3 cut(s) 572, 1307, 2412
Aor13HI TCCGGA 2 cut(s) 149, 404
AoxI GGCC 5 cut(s) 109, 523, 682, 1612, 2107
ApeKI GCWGC 9 cut(s) 7, 55, 58, 487, 856, 868, 959, 1562, 2396
ApoI RAATTY 9 cut(s) 307, 577, 1019, 1380, 1440, 1697, 1826, 1998, 2386
AseI ATTAAT 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 1004
Asp700I GAANNNNTTC 2 cut(s) 1191, 2024
AspLEI GCGC 1 cut(s) 537
AspS9I GGNCC 6 cut(s) 283, 549, 844, 950, 1613, 1951
AsuC2I CCSGG 5 cut(s) 279, 492, 573, 574, 809
AsuHPI GGTGA 6 cut(s) 653, 835, 978, 1286, 2212, 2437
AvaI CYCGRG 3 cut(s) 572, 1307, 2412
AvaII GGWCC 5 cut(s) 283, 549, 844, 950, 1951
BalI TGGCCA 1 cut(s) 2109
BanI GGYRCC 2 cut(s) 534, 2250
BarI GAAGNNNNNNTAC 2 cut(s) 1504, 1536
BbsI GAAGAC 2 cut(s) 14, 2301
BbvI GCAGC 8 cut(s) 42, 45, 474, 843, 855, 946, 1549, 2383
BccI CCATC 3 cut(s) 296, 341, 1849
BceAI ACGGC 3 cut(s) 93, 697, 2092
BcgI CGANNNNNNTGC 8 cut(s) 360, 394, 838, 872, 920, 954, 1673, 1707
BciT130I CCWGG 2 cut(s) 108, 547
BclI TGATCA 4 cut(s) 466, 643, 760, 1525
BcnI CCSGG 5 cut(s) 279, 492, 573, 574, 809
BcoDI GTCTC 3 cut(s) 51, 252, 1429
BfaI CTAG 1 cut(s) 969
BfoI RGCGCY 1 cut(s) 538
BfuAI ACCTGC 2 cut(s) 1812, 2374
BisI GCNGC 9 cut(s) 8, 56, 59, 488, 857, 869, 960, 1563, 2397
BlsI GCNGC 9 cut(s) 9, 57, 60, 489, 858, 870, 961, 1564, 2398
BmcAI AGTACT 2 cut(s) 1908, 1925
Bme1390I CCNGG 7 cut(s) 108, 279, 492, 547, 573, 574, 809
Bme18I GGWCC 5 cut(s) 283, 549, 844, 950, 1951
BmeT110I CYCGRG 3 cut(s) 572, 1307, 2412
BmgT120I GGNCC 6 cut(s) 283, 549, 844, 950, 1613, 1951
BmiI GGNNCC 6 cut(s) 284, 536, 846, 915, 1952, 2252
BmrFI CCNGG 7 cut(s) 108, 279, 492, 547, 573, 574, 809
BmsI GCATC 2 cut(s) 362, 2426
BpiI GAAGAC 2 cut(s) 14, 2301
BpmI CTGGAG 4 cut(s) 676, 1524, 1912, 2271
BpuEI CTTGAG 1 cut(s) 1884
BpuMI CCSGG 5 cut(s) 279, 492, 573, 574, 809
BsaBI GATNNNNATC 2 cut(s) 1524, 1790
BsaHI GRCGYC 1 cut(s) 535
BsaJI CCNNGG 4 cut(s) 202, 278, 572, 807
BsaWI WCCGGW 4 cut(s) 149, 404, 1004, 1132
BsaXI ACNNNNNCTCC 4 cut(s) 1061, 1091, 1910, 1940
Bsc4I CCNNNNNNNGG 3 cut(s) 339, 572, 1662
Bse118I RCCGGY 1 cut(s) 1004
Bse1I ACTGG 3 cut(s) 659, 917, 1057
Bse8I GATNNNNATC 2 cut(s) 1524, 1790
BseAI TCCGGA 2 cut(s) 149, 404
BseBI CCWGG 2 cut(s) 108, 547
BseDI CCNNGG 4 cut(s) 202, 278, 572, 807
BseJI GATNNNNATC 2 cut(s) 1524, 1790
BseLI CCNNNNNNNGG 3 cut(s) 339, 572, 1662
BseMII CTCAG 7 cut(s) 284, 489, 730, 1164, 1440, 2082, 2109
BseNI ACTGG 3 cut(s) 659, 917, 1057
BseRI GAGGAG 1 cut(s) 1790
BseXI GCAGC 8 cut(s) 42, 45, 474, 843, 855, 946, 1549, 2383
BshFI GGCC 5 cut(s) 111, 525, 684, 1614, 2109
BshNI GGYRCC 2 cut(s) 534, 2250
BshTI ACCGGT 1 cut(s) 1004
BsiHKCI CYCGRG 3 cut(s) 572, 1307, 2412
BsiSI CCGG 8 cut(s) 150, 278, 405, 492, 573, 809, 1005, 1133
BslFI GGGAC 3 cut(s) 1642, 1964, 2071
BslI CCNNNNNNNGG 3 cut(s) 339, 572, 1662
BsmAI GTCTC 3 cut(s) 51, 252, 1429
BsmFI GGGAC 3 cut(s) 1642, 1964, 2071
BsmI GAATGC 2 cut(s) 1193, 2099
BsnI GGCC 5 cut(s) 111, 525, 684, 1614, 2109
BsoBI CYCGRG 3 cut(s) 572, 1307, 2412
Bsp13I TCCGGA 2 cut(s) 149, 404
BspACI CCGC 3 cut(s) 266, 552, 1201
BspANI GGCC 5 cut(s) 111, 525, 684, 1614, 2109
BspCNI CTCAG 7 cut(s) 285, 488, 729, 1165, 1439, 2083, 2110
BspEI TCCGGA 2 cut(s) 149, 404
BspHI TCATGA 3 cut(s) 757, 766, 1333
BspLI GGNNCC 6 cut(s) 284, 536, 846, 915, 1952, 2252
BspMI ACCTGC 2 cut(s) 1812, 2374
BspPI GGATC 4 cut(s) 557, 1143, 1293, 1832
BspT107I GGYRCC 2 cut(s) 534, 2250
BsrBI CCGCTC 2 cut(s) 268, 1203
BsrFI RCCGGY 1 cut(s) 1004
BsrI ACTGG 3 cut(s) 659, 917, 1057
BssAI RCCGGY 1 cut(s) 1004
BssECI CCNNGG 4 cut(s) 202, 278, 572, 807
BssNI GRCGYC 1 cut(s) 535
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 2 cut(s) 108, 547
Bst4CI ACNGT 6 cut(s) 906, 982, 1274, 1361, 1483, 1974
Bst6I CTCTTC 2 cut(s) 426, 1767
BstACI GRCGYC 1 cut(s) 535
BstC8I GCNNGC 5 cut(s) 82, 527, 589, 609, 2324
BstDEI CTNAG 8 cut(s) 293, 475, 716, 1173, 1426, 2091, 2118, 2246
BstH2I RGCGCY 1 cut(s) 538
BstHHI GCGC 1 cut(s) 537
BstMAI GTCTC 3 cut(s) 51, 252, 1429
BstMWI GCNNNNNNNGC 3 cut(s) 865, 874, 1232
BstNI CCWGG 2 cut(s) 108, 547
BstNSI RCATGY 3 cut(s) 2086, 2229, 2326
BstSCI CCNGG 7 cut(s) 106, 277, 490, 545, 571, 572, 807
BstV1I GCAGC 8 cut(s) 42, 45, 474, 843, 855, 946, 1549, 2383
BstV2I GAAGAC 2 cut(s) 14, 2301
BstX2I RGATCY 1 cut(s) 1285
BstXI CCANNNNNNTGG 1 cut(s) 243
BstYI RGATCY 1 cut(s) 1285
BsuRI GGCC 5 cut(s) 111, 525, 684, 1614, 2109
BtsIMutI CAGTG 1 cut(s) 724
BveI ACCTGC 2 cut(s) 1812, 2374
Cac8I GCNNGC 5 cut(s) 82, 527, 589, 609, 2324
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 3 cut(s) 757, 766, 1333
CfoI GCGC 1 cut(s) 537
Cfr10I RCCGGY 1 cut(s) 1004
Cfr13I GGNCC 6 cut(s) 283, 549, 844, 950, 1613, 1951
Cfr9I CCCGGG 1 cut(s) 572
Csp6I GTAC 9 cut(s) 75, 104, 214, 966, 1013, 1270, 1801, 1907, 1924
CspAI ACCGGT 1 cut(s) 1004
CviQI GTAC 9 cut(s) 75, 104, 214, 966, 1013, 1270, 1801, 1907, 1924
DdeI CTNAG 8 cut(s) 293, 475, 716, 1173, 1426, 2091, 2118, 2246
DinI GGCGCC 1 cut(s) 536
EaeI YGGCCR 2 cut(s) 682, 2107
Eam1104I CTCTTC 2 cut(s) 426, 1767
EarI CTCTTC 2 cut(s) 426, 1767
Eco130I CCWWGG 1 cut(s) 202
Eco32I GATATC 1 cut(s) 1463
Eco47I GGWCC 5 cut(s) 283, 549, 844, 950, 1951
Eco88I CYCGRG 3 cut(s) 572, 1307, 2412
EcoO109I RGGNCCY 2 cut(s) 283, 1951
EcoRI GAATTC 3 cut(s) 1440, 1697, 2386
EcoRII CCWGG 2 cut(s) 106, 545
EcoRV GATATC 1 cut(s) 1463
EcoT14I CCWWGG 1 cut(s) 202
EcoT22I ATGCAT 2 cut(s) 355, 2101
EgeI GGCGCC 1 cut(s) 536
EheI GGCGCC 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 202
FalI AAGNNNNNCTT 4 cut(s) 118, 150, 1893, 1925
FaqI GGGAC 3 cut(s) 1642, 1964, 2071
FbaI TGATCA 4 cut(s) 466, 643, 760, 1525
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 9 cut(s) 8, 56, 59, 488, 857, 869, 960, 1563, 2397
Fsp4HI GCNGC 9 cut(s) 8, 56, 59, 488, 857, 869, 960, 1563, 2397
FspBI CTAG 1 cut(s) 969
GlaI GCGC 1 cut(s) 536
GluI GCNGC 9 cut(s) 8, 56, 59, 488, 857, 869, 960, 1563, 2397
GsuI CTGGAG 4 cut(s) 676, 1524, 1912, 2271
HaeII RGCGCY 1 cut(s) 538
HaeIII GGCC 5 cut(s) 111, 525, 684, 1614, 2109
HapII CCGG 8 cut(s) 150, 278, 405, 492, 573, 809, 1005, 1133
HhaI GCGC 1 cut(s) 537
Hin1I GRCGYC 1 cut(s) 535
Hin6I GCGC 1 cut(s) 535
HinP1I GCGC 1 cut(s) 535
HinfI GANTC 6 cut(s) 260, 401, 1297, 1810, 2035, 2210
HpaII CCGG 8 cut(s) 150, 278, 405, 492, 573, 809, 1005, 1133
HphI GGTGA 6 cut(s) 653, 835, 978, 1286, 2212, 2437
Hpy166II GTNNAC 4 cut(s) 189, 670, 902, 1222
Hpy188I TCNGA 9 cut(s) 27, 478, 1402, 1582, 1598, 1846, 2005, 2119, 2236
Hpy8I GTNNAC 4 cut(s) 189, 670, 902, 1222
HpyAV CCTTC 8 cut(s) 622, 1066, 1094, 1202, 1487, 1506, 1883, 2177
HpyCH4III ACNGT 6 cut(s) 906, 982, 1274, 1361, 1483, 1974
HpyF10VI GCNNNNNNNGC 3 cut(s) 865, 874, 1232
HpyF3I CTNAG 8 cut(s) 293, 475, 716, 1173, 1426, 2091, 2118, 2246
Hsp92I GRCGYC 1 cut(s) 535
HspAI GCGC 1 cut(s) 535
KasI GGCGCC 1 cut(s) 534
Kpn2I TCCGGA 2 cut(s) 149, 404
Ksp22I TGATCA 4 cut(s) 466, 643, 760, 1525
LmnI GCTCC 5 cut(s) 809, 913, 946, 1734, 2290
Lsp1109I GCAGC 8 cut(s) 42, 45, 474, 843, 855, 946, 1549, 2383
LweI GCATC 2 cut(s) 362, 2426
MaeI CTAG 1 cut(s) 969
MaeIII GTNAC 4 cut(s) 984, 1000, 1274, 1355
MbiI CCGCTC 2 cut(s) 268, 1203
MfeI CAATTG 4 cut(s) 237, 384, 699, 1419
MflI RGATCY 1 cut(s) 1285
MlsI TGGCCA 1 cut(s) 2109
MluNI TGGCCA 1 cut(s) 2109
Mly113I GGCGCC 1 cut(s) 535
MlyI GAGTC 1 cut(s) 254
MmeI TCCRAC 9 cut(s) 417, 822, 1095, 1132, 1756, 1956, 2047, 2214, 2457
Mox20I TGGCCA 1 cut(s) 2109
Mph1103I ATGCAT 2 cut(s) 355, 2101
MroI TCCGGA 2 cut(s) 149, 404
MroXI GAANNNNTTC 2 cut(s) 1191, 2024
MscI TGGCCA 1 cut(s) 2109
MseI TTAA 5 cut(s) 32, 795, 1079, 1547, 1659
MslI CAYNNNNRTG 3 cut(s) 771, 994, 1338
Msp20I TGGCCA 1 cut(s) 2109
MspI CCGG 8 cut(s) 150, 278, 405, 492, 573, 809, 1005, 1133
MspR9I CCNGG 7 cut(s) 108, 279, 492, 547, 573, 574, 809
MunI CAATTG 4 cut(s) 237, 384, 699, 1419
Mva1269I GAATGC 2 cut(s) 1193, 2099
MvaI CCWGG 2 cut(s) 108, 547
MwoI GCNNNNNNNGC 3 cut(s) 865, 874, 1232
NarI GGCGCC 1 cut(s) 535
NciI CCSGG 5 cut(s) 279, 492, 573, 574, 809
NlaIV GGNNCC 6 cut(s) 284, 536, 846, 915, 1952, 2252
NmuCI GTSAC 4 cut(s) 984, 1000, 1274, 1355
NsiI ATGCAT 2 cut(s) 355, 2101
NspI RCATGY 3 cut(s) 2086, 2229, 2326
PaeI GCATGC 1 cut(s) 2326
PaeR7I CTCGAG 1 cut(s) 2412
PagI TCATGA 3 cut(s) 757, 766, 1333
PciI ACATGT 1 cut(s) 2082
PcsI WCGNNNNNNNCGW 1 cut(s) 927
PctI GAATGC 2 cut(s) 1193, 2099
PdmI GAANNNNTTC 2 cut(s) 1191, 2024
PfeI GAWTC 5 cut(s) 401, 1297, 1810, 2035, 2210
PflMI CCANNNNNTGG 1 cut(s) 339
PinAI ACCGGT 1 cut(s) 1004
PkrI GCNGC 9 cut(s) 9, 57, 60, 489, 858, 870, 961, 1564, 2398
PleI GAGTC 1 cut(s) 254
PluTI GGCGCC 1 cut(s) 538
PpsI GAGTC 1 cut(s) 254
PpuMI RGGWCCY 2 cut(s) 283, 1951
PscI ACATGT 1 cut(s) 2082
PshBI ATTAAT 1 cut(s) 32
PsiI TTATAA 1 cut(s) 1623
Psp5II RGGWCCY 2 cut(s) 283, 1951
Psp6I CCWGG 2 cut(s) 106, 545
PspGI CCWGG 2 cut(s) 106, 545
PspN4I GGNNCC 6 cut(s) 284, 536, 846, 915, 1952, 2252
PspPI GGNCC 6 cut(s) 283, 549, 844, 950, 1613, 1951
PspPPI RGGWCCY 2 cut(s) 283, 1951
PspXI VCTCGAGB 1 cut(s) 2412
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 1285
RsaI GTAC 9 cut(s) 76, 105, 215, 967, 1014, 1271, 1802, 1908, 1925
RsaNI GTAC 9 cut(s) 75, 104, 214, 966, 1013, 1270, 1801, 1907, 1924
RseI CAYNNNNRTG 3 cut(s) 771, 994, 1338
SaqAI TTAA 5 cut(s) 32, 795, 1079, 1547, 1659
SatI GCNGC 9 cut(s) 8, 56, 59, 488, 857, 869, 960, 1563, 2397
Sau96I GGNCC 6 cut(s) 283, 549, 844, 950, 1613, 1951
ScaI AGTACT 2 cut(s) 1908, 1925
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 7 cut(s) 108, 279, 492, 547, 573, 574, 809
SfaNI GCATC 2 cut(s) 362, 2426
SfoI GGCGCC 1 cut(s) 536
Sfr274I CTCGAG 1 cut(s) 2412
SinI GGWCC 5 cut(s) 283, 549, 844, 950, 1951
SlaI CTCGAG 1 cut(s) 2412
SmaI CCCGGG 1 cut(s) 574
SmiMI CAYNNNNRTG 3 cut(s) 771, 994, 1338
SmlI CTYRAG 2 cut(s) 1899, 2412
SmoI CTYRAG 2 cut(s) 1899, 2412
SphI GCATGC 1 cut(s) 2326
SsiI CCGC 3 cut(s) 266, 552, 1201
SspDI GGCGCC 1 cut(s) 534
SspI AATATT 1 cut(s) 197
SspMI CTAG 1 cut(s) 969
StyD4I CCNGG 7 cut(s) 106, 277, 490, 545, 571, 572, 807
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 6 cut(s) 906, 982, 1274, 1361, 1483, 1974
TaqI TCGA 9 cut(s) 380, 921, 1090, 1693, 1813, 1836, 2043, 2296, 2413
TatI WGTACW 3 cut(s) 965, 1906, 1923
TfiI GAWTC 5 cut(s) 401, 1297, 1810, 2035, 2210
Tru1I TTAA 5 cut(s) 32, 795, 1079, 1547, 1659
Tru9I TTAA 5 cut(s) 32, 795, 1079, 1547, 1659
TscAI CASTG 1 cut(s) 724
TseFI GTSAC 4 cut(s) 984, 1000, 1274, 1355
TseI GCWGC 9 cut(s) 7, 55, 58, 487, 856, 868, 959, 1562, 2396
Tsp45I GTSAC 4 cut(s) 984, 1000, 1274, 1355
TspGWI ACGGA 1 cut(s) 164
TspMI CCCGGG 1 cut(s) 572
TspRI CASTG 1 cut(s) 724
Van91I CCANNNNNTGG 1 cut(s) 339
VpaK11BI GGWCC 5 cut(s) 283, 549, 844, 950, 1951
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 9 cut(s) 307, 577, 1019, 1380, 1440, 1697, 1826, 1998, 2386
XceI RCATGY 3 cut(s) 2086, 2229, 2326
XhoI CTCGAG 1 cut(s) 2412
XmaI CCCGGG 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 669
XmnI GAANNNNTTC 2 cut(s) 1191, 2024
XspI CTAG 1 cut(s) 969
ZrmI AGTACT 2 cut(s) 1908, 1925
Zsp2I ATGCAT 2 cut(s) 355, 2101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.