Rroxscaffold_2G00089370

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000114
Physical Location & Seq
Forward (+)
11400069 .. 11403740
3672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00089370.1

Sequence Viewer

Length: 2055 bp
ATGGAGTGGTGCCTACCGAACCAATGTTCTCCTAGACACTTTGACCTGCAAGAAAGAAAACCAGAGGAAGAAGGGAAAAACAAATTTCCTCACTACTCTACCTTTGTTATTTTAATGATCAGTCTCTGGTCAGTGCCGTGGTCTATCAAATTGCTGTGTATCCCAGGTACGCCATTTAGCCAGGACACTATAATACCAGGCAAATCTATCAGTGCAAACCAGACATTAATTTCTTCCAATGGAACCTTTAGATTAGGTTTTTTCAGTCCTGCAAATTCTACCAAATTTTTCCTAGGTATATGGTACAACACAATCCCACACACTGCATCAATTGTATGGGTAGCCAACAGAGAATCCCCACTTGAATCTCCAGGTTTTTTTGCCCTCAGAGGTGATGGGAATCTTGTGGTGTTGGATGGAACTGGAAAAGTACTTATCTGGTCATCTAATGCATCAGTTGCTTCTTCTGCAAGGAATGCCACAACTGGTTTCCTAATGAATAACGGAAACCTCGTTCTGAGGTTTGGAGAAGGCATTTTGTGGCAGAGCTTTGATCATCCTTCTGATACATTGTTGCCTGAAATGAAGGTTAGCTTGAACAAAAGGAGTGGCCAACAAAGGTGCCTTACATCCTGGGCAGCGCTTGATGATCCACAACCAGGAAAGTTCACTTTAGGCTTTGATCCTAAAGGACTACCAGGGCAGGCTTATATCTGGAAGGAAAATGCTCCATATTGGAGAAGTGGTACCTTCTTTGGCAAGGAGACGAAAACAAACTTTGGAATTTCAACTGAAAACTCGTATTTCCTCACTTACAACTTTGACGCTGATGAGGACTATCTTACTTATGGTGTCTCGGTGTCGGTTAGTCCAGTAAAATTGAGGGCCTTGCTGGATCCAACTGGGCAGATTGTGATGCAGCAATGGCTAGTTCACAGTAGAACATGGTATGCATGGTGGAGGGAGCCGGTTCATAAATGTGATTTTTATGCTCCTTGTGGTCCATTTGGTGCCTGTGATAAAAATGAGTCCCTCTCATCTCCATGTAAATGTTTGACAGGTTTTATACCCAAATTTCAGAAACAATGGGCTCAGGGGGATTGGGCTGGTGGATGTGTCAGGGAAAAACCACTGACATGCGATAGCAGATCAGAAGGATTTTCAAGGTTTGAAGGGTTGAAACTACCAGATCATGCTGTTCTTTTAGTCAATAAGAGTATGAGCGAGTGTGAGTCTCAATGCTATCAGAACTGTTCTTGCACAGCTTATGCTTACCTGAATGAGACAGGAGAAAGTACCGCGAGATGTTTGACATGGTTTGGTGACTTGATTGATCTTGTAGCAAATCACTCTCTTGGGAAAAATATTTTTATTCGTGTTCATCGCTCCTATCAAGGTATGGGGATTTTAACTGAAAATCAAGAAGTAGCCATAAAAAGGCTATCCACGAAGTCCGGGCAAGGACATCAGGAGTTCATGAATGAGTTAAAGCTTATAGCTAAACTCCAGCATACCAATCTTGTTAGGCTCTTGGGTTGCTGTATCGAAGAAGAGGAAAGGGTATTGATGTATGAGTACATGCCCAATCGAAGTTTAGACAACGGCTACATGTCACCTGAATACGCTCTCTATGGTCATTTTTCTGATAAAGTTGATGTATTTAGCTTTGGGGTGTTGTTGCTGGAGATTGTAAGTGGAAGGAAGAATGCTATTTTTCATCATTTTGATCGTTCACTAACTCTTGCTGGATGGGTATGGGAGTTATGGAAGGAAGGAAGAGGAGTGGAGGTGATTGATGCATCAATAAGGGAAACATGCCAGTTTCATGAGGCGCCTCTAAGGTGTATCCATGTAGGGCTCTTGTGTGTTCAACAAGCTGCAGCTGATCGACCAACAATGTCCTCGGTAATTCTTATGCTGCAAGGCACTGGAGTTTCATCACTTCCACCTTCCAATGAACCTGCCTTTTCAACACTTGAGAACGCCAATGTTGTTGGTTCATCTACTAGTTTTTCCAATAATGAAATTACCATTAGTTTACTAGAAGCTCGGTAG

Protein Analysis

684

Amino Acids

76.66

Weight (kDa)

6.18

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 111 - 212 1.7e-28 D-mannose binding lectin
S_locus_glycop PF00954 246 - 358 4.5e-19 S-locus glycoprotein domain
PAN_2 PF08276 380 - 446 2.1e-15 PAN-like domain
PK_Tyr_Ser-Thr PF07714 449 - 534 2.3e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 471 - 533 3.8e-08 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 536 - 637 2e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000114)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_4g01160 FvH4_6g34940 FvH4_6g44062 FvH4_6g44106 FvH4_6g44106 FvH4_6g44106 FvH4_6g44107 FvH4_6g44107 FvH4_6g44109 FvH4_6g44140 FvH4_6g44190 FvH4_6g44200 FvH4_6g44240 FvH4_6g44241 FvH4_6g44242
malus_domestica MD09G1094900.v1.1 MD09G1100300.v1.1 MD09G1100400.v1.1 MD09G1100600.v1.1 MD09G1100800.v1.1 MD09G1100900.v1.1 MD09G1101300.v1.1 MD09G1255200.v1.1 MD17G1088400.v1.1 MD17G1088600.v1.1 MD17G1247900.v1.1 MD17G1248300.v1.1
prunus_persica Prupe.1G497000_v2.0.a1 Prupe.2G033300_v2.0.a1 Prupe.3G145500_v2.0.a1 Prupe.3G225400_v2.0.a1 Prupe.3G225700_v2.0.a1 Prupe.3G225700_v2.0.a1
pyrus_communis pycom06g12720 pycom06g12740 pycom09g01960 pycom09g02450 pycom17g08520 pycom17g08530 pycom17g08560 pycom17g24950 pycom17g25030 pycom17g25110 pycom17g25260 pycom17g25290
rosa_chinensis RchiOBHm_Chr1g0326521 RchiOBHm_Chr1g0326551 RchiOBHm_Chr1g0326591 RchiOBHm_Chr1g0328741 RchiOBHm_Chr1g0329371 RchiOBHm_Chr2g0145911 RchiOBHm_Chr2g0160891 RchiOBHm_Chr2g0160921 RchiOBHm_Chr2g0161001 RchiOBHm_Chr2g0161011 RchiOBHm_Chr2g0161431 RchiOBHm_Chr2g0165121 RchiOBHm_Chr2g0166301 RchiOBHm_Chr4g0399551 RchiOBHm_Chr4g0405681 RchiOBHm_Chr5g0066741 RchiOBHm_Chr5g0066841 RchiOBHm_Chr6g0273021 RchiOBHm_Chr7g0225151
rosa_laevigata RLG00000001843 RLG00000008827 RLG00000014283 RLG00000014284 RLG00000020173 RLG00000021244 RLG00000021246 RLG00000021248 RLG00000021283 RLG00000023606 RLG00000029921 RLG00000030066 RLG00000030067 RLG00000033456
rosa_multiflora Rmu_co7958161.1_g000001 Rmu_co8440151.1_g000001 Rmu_co8454989.1_g000001 Rmu_sc0000129.1_g000018 Rmu_sc0000322.1_g000011 Rmu_sc0000322.1_g000045 Rmu_sc0000605.1_g000051 Rmu_sc0000955.1_g000015 Rmu_sc0000955.1_g000020 Rmu_sc0001188.1_g000017 Rmu_sc0001188.1_g000019 Rmu_sc0001355.1_g000015 Rmu_sc0001702.1_g000005 Rmu_sc0001702.1_g000006 Rmu_sc0001702.1_g000007 Rmu_sc0001702.1_g000008 Rmu_sc0001702.1_g000009 Rmu_sc0001702.1_g000010 Rmu_sc0001702.1_g000011 Rmu_sc0001702.1_g000012 Rmu_sc0001702.1_g000013 Rmu_sc0001702.1_g000014 Rmu_sc0001702.1_g000015 Rmu_sc0001702.1_g000016 Rmu_sc0002473.1_g000012 Rmu_sc0002473.1_g000015 Rmu_sc0002773.1_g000029 Rmu_sc0004178.1_g000002 Rmu_sc0005189.1_g000006 Rmu_sc0005860.1_g000004 Rmu_sc0009205.1_g000005 Rmu_sc0009205.1_g000007 Rmu_sc0009205.1_g000023 Rmu_sc0009472.1_g000005 Rmu_sc0009858.1_g000015 Rmu_sc0020187.1_g000001
rosa_roxburghii Rroxscaffold_176G00431460 Rroxscaffold_176G00431480 Rroxscaffold_1G00014330 Rroxscaffold_1G00031590 Rroxscaffold_2G00088820 Rroxscaffold_2G00089330 Rroxscaffold_2G00089360 Rroxscaffold_2G00089370 Rroxscaffold_2G00089380 Rroxscaffold_2G00100370 Rroxscaffold_2G00100380 Rroxscaffold_4G00321410 Rroxscaffold_4G00323700 Rroxscaffold_4G00327640 Rroxscaffold_4G00327650 Rroxscaffold_5G00344450 Rroxscaffold_7G00202770 Rroxscaffold_7G00203450 Rroxscaffold_7G00203540
rosa_rugosa Rorug01G0061100 Rorug02G0482100 Rorug02G0485900 Rorug02G0485900 Rorug02G0489500 Rorug02G0489600 Rorug02G0489700 Rorug04G0016600 Rorug04G0016700 Rorug05G0379000 Rorug05G0516700 Rorug06G0007900 Rorug06G0009300
rosa_samantha Rh1AG076700 Rh1AG076800 Rh1AG077000 Rh1AG077300 Rh1AG094400 Rh1AG097500 Rh1AG402000 Rh1BG062700 Rh1BG062900 Rh1CG075500 Rh1CG075600 Rh1CG075800 Rh1CG090900 Rh1CG091400 Rh1CG091600 Rh1CG094100 Rh1DG082300 Rh1DG082500 Rh1DG097400 Rh2AG448200 Rh2AG448300 Rh2AG511600 Rh2AG547500 Rh2AG551400 Rh2AG551700 Rh2AG555800 Rh2AG588000 Rh2BG565900 Rh2BG566000 Rh2BG566300 Rh2BG569000 Rh2BG600000 Rh2CG435200 Rh2CG435300 Rh2CG496600 Rh2CG535400 Rh2CG535600 Rh2CG539700 Rh2CG562700 Rh2CG570900 Rh2DG574600 Rh2DG574800 Rh2DG578600 Rh2DG610200 Rh4CG140900 Rh4DG127800 Rh5AG438500 Rh5BG436500 Rh5BG455500 Rh5CG477600 Rh5DG470300 Rh6AG129500 Rh6AG129900 Rh6BG125700 Rh6BG126000 Rh6BG126700 Rh6BG126800 Rh6CG124500 Rh6CG124800 Rh6CG125200 Rh6CG125300 Rh6DG111400 Rh6DG111700 Rh6DG111800 Rh7DG373300
rosa_wichuraiana Rw0G014010 Rw1G006140 Rw1G006160 Rw1G006180 Rw1G006190 Rw1G007340 Rw1G007360 Rw2G045660 Rw2G045670 Rw2G045970 Rw2G048220 Rw4G010820 Rw5G041010 Rw6G011220 Rw6G011230 Rw7G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 54, 1969
Acc65I GGTACC 1 cut(s) 746
AccB1I GGYRCC 5 cut(s) 9, 621, 746, 1010, 1831
AccII CGCG 1 cut(s) 1301
AciI CCGC 1 cut(s) 1299
AclWI GGATC 4 cut(s) 644, 677, 890, 903
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 5 cut(s) 83, 274, 284, 783, 1073
AcyI GRCGYC 1 cut(s) 1832
AfaI GTAC 6 cut(s) 169, 305, 432, 748, 1297, 1577
AfeI AGCGCT 1 cut(s) 642
AfiI CCNNNNNNNGG 2 cut(s) 659, 1437
AflIII ACRYGT 1 cut(s) 1608
AgsI TTSAA 8 cut(s) 365, 598, 789, 1164, 1172, 1180, 1871, 1971
AhlI ACTAGT 1 cut(s) 2006
AjnI CCWGG 7 cut(s) 163, 180, 196, 370, 632, 658, 697
AloI GAACNNNNNNTCC 2 cut(s) 498, 530
AluBI AGCT 9 cut(s) 549, 594, 1265, 1492, 1499, 1665, 1877, 1883, 2048
AluI AGCT 9 cut(s) 549, 594, 1265, 1492, 1499, 1665, 1877, 1883, 2048
Alw26I GTCTC 5 cut(s) 128, 758, 859, 1239, 1277
AlwI GGATC 4 cut(s) 644, 677, 890, 903
AlwNI CAGNNNCTG 1 cut(s) 126
Aor51HI AGCGCT 1 cut(s) 642
AoxI GGCC 2 cut(s) 610, 885
ApeKI GCWGC 5 cut(s) 638, 919, 1877, 1880, 1918
ApoI RAATTY 5 cut(s) 83, 274, 284, 783, 1073
AseI ATTAAT 1 cut(s) 227
Asp718I GGTACC 1 cut(s) 746
AspA2I CCTAGG 1 cut(s) 292
AspLEI GCGC 2 cut(s) 643, 1834
AspS9I GGNCC 2 cut(s) 885, 1001
AsuC2I CCSGG 1 cut(s) 1456
AsuHPI GGTGA 4 cut(s) 404, 1334, 1605, 1801
AvaII GGWCC 1 cut(s) 1001
AvrII CCTAGG 1 cut(s) 292
BalI TGGCCA 1 cut(s) 612
BamHI GGATCC 1 cut(s) 895
BanI GGYRCC 5 cut(s) 9, 621, 746, 1010, 1831
BanII GRGCYC 2 cut(s) 1093, 1860
BbvI GCAGC 5 cut(s) 650, 931, 1864, 1892, 1905
BccI CCATC 3 cut(s) 389, 410, 1743
BceAI ACGGC 2 cut(s) 121, 1618
BciT130I CCWGG 7 cut(s) 165, 182, 198, 372, 634, 660, 699
BciVI GTATCC 2 cut(s) 170, 1856
BclI TGATCA 2 cut(s) 117, 553
BcnI CCSGG 1 cut(s) 1456
BcoDI GTCTC 5 cut(s) 128, 758, 859, 1239, 1277
BcuI ACTAGT 1 cut(s) 2006
BfaI CTAG 5 cut(s) 33, 293, 929, 2007, 2042
BfmI CTRYAG 1 cut(s) 1878
BfoI RGCGCY 2 cut(s) 644, 1835
BfuAI ACCTGC 2 cut(s) 54, 1969
BfuI GTATCC 2 cut(s) 170, 1856
BisI GCNGC 5 cut(s) 639, 920, 1878, 1881, 1919
BlnI CCTAGG 1 cut(s) 292
BlsI GCNGC 5 cut(s) 640, 921, 1879, 1882, 1920
BmcAI AGTACT 1 cut(s) 432
Bme1390I CCNGG 8 cut(s) 165, 182, 198, 372, 634, 660, 699, 1456
Bme18I GGWCC 1 cut(s) 1001
BmgT120I GGNCC 2 cut(s) 885, 1001
BmiI GGNNCC 8 cut(s) 11, 244, 623, 748, 897, 966, 1012, 1833
BmrFI CCNGG 8 cut(s) 165, 182, 198, 372, 634, 660, 699, 1456
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 5 cut(s) 335, 461, 906, 1786, 1808
BmuI ACTGGG 1 cut(s) 912
BplI GAGNNNNNCTC 2 cut(s) 1019, 1051
BpmI CTGGAG 4 cut(s) 354, 1490, 1703, 1950
Bpu10I CCTNAGC 1 cut(s) 1092
BpuEI CTTGAG 1 cut(s) 1997
BpuMI CCSGG 1 cut(s) 1456
BsaBI GATNNNNATC 1 cut(s) 399
BsaHI GRCGYC 1 cut(s) 1832
BsaJI CCNNGG 6 cut(s) 137, 163, 292, 633, 698, 1902
BsaXI ACNNNNNCTCC 4 cut(s) 730, 760, 1773, 1803
Bsc4I CCNNNNNNNGG 2 cut(s) 659, 1437
Bse118I RCCGGY 1 cut(s) 967
Bse1I ACTGG 6 cut(s) 427, 490, 872, 907, 1819, 1933
Bse3DI GCAATG 1 cut(s) 929
Bse8I GATNNNNATC 1 cut(s) 399
BseBI CCWGG 7 cut(s) 165, 182, 198, 372, 634, 660, 699
BseDI CCNNGG 6 cut(s) 137, 163, 292, 633, 698, 1902
BseGI GGATG 5 cut(s) 421, 556, 629, 1118, 1754
BseJI GATNNNNATC 1 cut(s) 399
BseLI CCNNNNNNNGG 2 cut(s) 659, 1437
BseMI GCAATG 1 cut(s) 929
BseMII CTCAG 3 cut(s) 400, 509, 1106
BseNI ACTGG 6 cut(s) 427, 490, 872, 907, 1819, 1933
BseRI GAGGAG 1 cut(s) 1794
BseXI GCAGC 5 cut(s) 650, 931, 1864, 1892, 1905
Bsh1236I CGCG 1 cut(s) 1301
BshFI GGCC 2 cut(s) 612, 887
BshNI GGYRCC 5 cut(s) 9, 621, 746, 1010, 1831
BsiSI CCGG 2 cut(s) 968, 1455
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 2 cut(s) 659, 1437
BsmAI GTCTC 5 cut(s) 128, 758, 859, 1239, 1277
BsmBI CGTCTC 1 cut(s) 758
BsmFI GGGAC 1 cut(s) 1015
BsmI GAATGC 2 cut(s) 481, 1711
BsnI GGCC 2 cut(s) 612, 887
Bsp1286I GDGCHC 2 cut(s) 1093, 1860
BspACI CCGC 1 cut(s) 1299
BspANI GGCC 2 cut(s) 612, 887
BspCNI CTCAG 3 cut(s) 399, 510, 1105
BspFNI CGCG 1 cut(s) 1301
BspHI TCATGA 2 cut(s) 1476, 1825
BspLI GGNNCC 8 cut(s) 11, 244, 623, 748, 897, 966, 1012, 1833
BspMAI CTGCAG 1 cut(s) 1882
BspMI ACCTGC 2 cut(s) 54, 1969
BspPI GGATC 4 cut(s) 644, 677, 890, 903
BspT107I GGYRCC 5 cut(s) 9, 621, 746, 1010, 1831
BsrDI GCAATG 1 cut(s) 929
BsrFI RCCGGY 1 cut(s) 967
BsrI ACTGG 6 cut(s) 427, 490, 872, 907, 1819, 1933
BssAI RCCGGY 1 cut(s) 967
BssECI CCNNGG 6 cut(s) 137, 163, 292, 633, 698, 1902
BssNI GRCGYC 1 cut(s) 1832
BssT1I CCWWGG 1 cut(s) 292
Bst2UI CCWGG 7 cut(s) 165, 182, 198, 372, 634, 660, 699
Bst4CI ACNGT 2 cut(s) 938, 1253
Bst6I CTCTTC 2 cut(s) 1545, 1771
BstACI GRCGYC 1 cut(s) 1832
BstAPI GCANNNNNTGC 2 cut(s) 458, 476
BstC8I GCNNGC 1 cut(s) 705
BstDEI CTNAG 4 cut(s) 386, 518, 1092, 1838
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 5 cut(s) 421, 556, 629, 1118, 1754
BstFNI CGCG 1 cut(s) 1301
BstH2I RGCGCY 2 cut(s) 644, 1835
BstHHI GCGC 2 cut(s) 643, 1834
BstMAI GTCTC 5 cut(s) 128, 758, 859, 1239, 1277
BstMWI GCNNNNNNNGC 4 cut(s) 458, 467, 476, 925
BstNI CCWGG 7 cut(s) 165, 182, 198, 372, 634, 660, 699
BstNSI RCATGY 4 cut(s) 1140, 1582, 1612, 1818
BstSCI CCNGG 8 cut(s) 163, 180, 196, 370, 632, 658, 697, 1454
BstSFI CTRYAG 1 cut(s) 1878
BstUI CGCG 1 cut(s) 1301
BstV1I GCAGC 5 cut(s) 650, 931, 1864, 1892, 1905
BstX2I RGATCY 1 cut(s) 895
BstYI RGATCY 1 cut(s) 895
BsuI GTATCC 2 cut(s) 170, 1856
BsuRI GGCC 2 cut(s) 612, 887
BtgI CCRYGG 1 cut(s) 137
BtgZI GCGATG 1 cut(s) 1367
BtsCI GGATG 5 cut(s) 421, 556, 629, 1118, 1754
BtsI GCAGTG 1 cut(s) 321
BtsIMutI CAGTG 5 cut(s) 138, 217, 321, 1130, 1926
BveI ACCTGC 2 cut(s) 54, 1969
Cac8I GCNNGC 1 cut(s) 705
CaiI CAGNNNCTG 1 cut(s) 126
CciI TCATGA 2 cut(s) 1476, 1825
CfoI GCGC 2 cut(s) 643, 1834
Cfr10I RCCGGY 1 cut(s) 967
Cfr13I GGNCC 2 cut(s) 885, 1001
CseI GACGC 1 cut(s) 833
Csp6I GTAC 6 cut(s) 168, 304, 431, 747, 1296, 1576
CspCI CAANNNNNGTGG 2 cut(s) 589, 624
CviQI GTAC 6 cut(s) 168, 304, 431, 747, 1296, 1576
DdeI CTNAG 4 cut(s) 386, 518, 1092, 1838
DinI GGCGCC 1 cut(s) 1833
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 2 cut(s) 1545, 1771
EarI CTCTTC 2 cut(s) 1545, 1771
Eco130I CCWWGG 1 cut(s) 292
Eco24I GRGCYC 2 cut(s) 1093, 1860
Eco47I GGWCC 1 cut(s) 1001
Eco47III AGCGCT 1 cut(s) 642
EcoO109I RGGNCCY 1 cut(s) 885
EcoRII CCWGG 7 cut(s) 163, 180, 196, 370, 632, 658, 697
EcoT14I CCWWGG 1 cut(s) 292
EcoT22I ATGCAT 3 cut(s) 454, 955, 1801
EcoT38I GRGCYC 2 cut(s) 1093, 1860
EgeI GGCGCC 1 cut(s) 1833
EheI GGCGCC 1 cut(s) 1833
ErhI CCWWGG 1 cut(s) 292
Esp3I CGTCTC 1 cut(s) 758
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 1015
FbaI TGATCA 2 cut(s) 117, 553
Fnu4HI GCNGC 5 cut(s) 639, 920, 1878, 1881, 1919
FokI GGATG 5 cut(s) 428, 543, 616, 1125, 1761
FriOI GRGCYC 2 cut(s) 1093, 1860
Fsp4HI GCNGC 5 cut(s) 639, 920, 1878, 1881, 1919
FspBI CTAG 5 cut(s) 33, 293, 929, 2007, 2042
GlaI GCGC 2 cut(s) 642, 1833
GluI GCNGC 5 cut(s) 639, 920, 1878, 1881, 1919
GsuI CTGGAG 4 cut(s) 354, 1490, 1703, 1950
HaeII RGCGCY 2 cut(s) 644, 1835
HaeIII GGCC 2 cut(s) 612, 887
HapII CCGG 2 cut(s) 968, 1455
HgaI GACGC 1 cut(s) 833
HhaI GCGC 2 cut(s) 643, 1834
Hin1I GRCGYC 1 cut(s) 1832
Hin6I GCGC 2 cut(s) 641, 1832
HinP1I GCGC 2 cut(s) 641, 1832
HindIII AAGCTT 1 cut(s) 1490
HinfI GANTC 5 cut(s) 353, 365, 400, 1028, 1232
HpaII CCGG 2 cut(s) 968, 1455
HphI GGTGA 4 cut(s) 404, 1334, 1605, 1801
Hpy166II GTNNAC 4 cut(s) 669, 934, 1733, 2039
Hpy188I TCNGA 7 cut(s) 389, 519, 565, 1080, 1153, 1248, 1645
Hpy188III TCNNGA 5 cut(s) 715, 1421, 1469, 1477, 1826
Hpy8I GTNNAC 4 cut(s) 669, 934, 1733, 2039
HpyCH4III ACNGT 2 cut(s) 938, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 458, 467, 476, 925
HpyF3I CTNAG 4 cut(s) 386, 518, 1092, 1838
Hsp92I GRCGYC 1 cut(s) 1832
HspAI GCGC 2 cut(s) 641, 1832
KasI GGCGCC 1 cut(s) 1831
KpnI GGTACC 1 cut(s) 750
Ksp22I TGATCA 2 cut(s) 117, 553
LmnI GCTCC 4 cut(s) 733, 964, 997, 1391
Lsp1109I GCAGC 5 cut(s) 650, 931, 1864, 1892, 1905
LweI GCATC 5 cut(s) 335, 461, 906, 1786, 1808
MaeI CTAG 5 cut(s) 33, 293, 929, 2007, 2042
MaeIII GTNAC 2 cut(s) 1322, 1611
MboII GAAGA 7 cut(s) 80, 225, 456, 1559, 1562, 1714, 1788
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 2 cut(s) 1093, 1860
MlsI TGGCCA 1 cut(s) 612
MluNI TGGCCA 1 cut(s) 612
Mly113I GGCGCC 1 cut(s) 1832
MlyI GAGTC 2 cut(s) 1037, 1241
MmeI TCCRAC 2 cut(s) 393, 923
Mox20I TGGCCA 1 cut(s) 612
Mph1103I ATGCAT 3 cut(s) 454, 955, 1801
MscI TGGCCA 1 cut(s) 612
MseI TTAA 4 cut(s) 113, 227, 1409, 1487
MslI CAYNNNNRTG 4 cut(s) 978, 1042, 1048, 1135
Msp20I TGGCCA 1 cut(s) 612
MspA1I CMGCKG 1 cut(s) 1883
MspI CCGG 2 cut(s) 968, 1455
MspR9I CCNGG 8 cut(s) 165, 182, 198, 372, 634, 660, 699, 1456
MunI CAATTG 1 cut(s) 330
Mva1269I GAATGC 2 cut(s) 481, 1711
MvaI CCWGG 7 cut(s) 165, 182, 198, 372, 634, 660, 699
MvnI CGCG 1 cut(s) 1301
MwoI GCNNNNNNNGC 4 cut(s) 458, 467, 476, 925
NarI GGCGCC 1 cut(s) 1832
NciI CCSGG 1 cut(s) 1456
NlaIV GGNNCC 8 cut(s) 11, 244, 623, 748, 897, 966, 1012, 1833
NmuCI GTSAC 2 cut(s) 1322, 1611
NsiI ATGCAT 3 cut(s) 454, 955, 1801
NspI RCATGY 4 cut(s) 1140, 1582, 1612, 1818
PagI TCATGA 2 cut(s) 1476, 1825
PciI ACATGT 1 cut(s) 1608
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PctI GAATGC 2 cut(s) 481, 1711
PfeI GAWTC 3 cut(s) 353, 365, 400
PkrI GCNGC 5 cut(s) 640, 921, 1879, 1882, 1920
PleI GAGTC 2 cut(s) 1036, 1240
PluTI GGCGCC 1 cut(s) 1835
PpsI GAGTC 2 cut(s) 1036, 1240
PscI ACATGT 1 cut(s) 1608
PshBI ATTAAT 1 cut(s) 227
Psp6I CCWGG 7 cut(s) 163, 180, 196, 370, 632, 658, 697
PspGI CCWGG 7 cut(s) 163, 180, 196, 370, 632, 658, 697
PspN4I GGNNCC 8 cut(s) 11, 244, 623, 748, 897, 966, 1012, 1833
PspPI GGNCC 2 cut(s) 885, 1001
PstI CTGCAG 1 cut(s) 1882
PstNI CAGNNNCTG 1 cut(s) 126
PsuI RGATCY 1 cut(s) 895
PvuII CAGCTG 1 cut(s) 1883
RsaI GTAC 6 cut(s) 169, 305, 432, 748, 1297, 1577
RsaNI GTAC 6 cut(s) 168, 304, 431, 747, 1296, 1576
RseI CAYNNNNRTG 4 cut(s) 978, 1042, 1048, 1135
SaqAI TTAA 4 cut(s) 113, 227, 1409, 1487
SatI GCNGC 5 cut(s) 639, 920, 1878, 1881, 1919
Sau96I GGNCC 2 cut(s) 885, 1001
ScaI AGTACT 1 cut(s) 432
SchI GAGTC 2 cut(s) 1037, 1241
ScrFI CCNGG 8 cut(s) 165, 182, 198, 372, 634, 660, 699, 1456
SduI GDGCHC 2 cut(s) 1093, 1860
SfaNI GCATC 5 cut(s) 335, 461, 906, 1786, 1808
SfcI CTRYAG 1 cut(s) 1878
SfoI GGCGCC 1 cut(s) 1833
SinI GGWCC 1 cut(s) 1001
SmiMI CAYNNNNRTG 4 cut(s) 978, 1042, 1048, 1135
SmlI CTYRAG 1 cut(s) 1976
SmoI CTYRAG 1 cut(s) 1976
SpeI ACTAGT 1 cut(s) 2006
SsiI CCGC 1 cut(s) 1299
SspDI GGCGCC 1 cut(s) 1831
SspI AATATT 1 cut(s) 1366
SspMI CTAG 5 cut(s) 33, 293, 929, 2007, 2042
StyD4I CCNGG 8 cut(s) 163, 180, 196, 370, 632, 658, 697, 1454
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 2 cut(s) 938, 1253
TaqI TCGA 3 cut(s) 1545, 1588, 1888
TatI WGTACW 2 cut(s) 430, 1575
TfiI GAWTC 3 cut(s) 353, 365, 400
Tru1I TTAA 4 cut(s) 113, 227, 1409, 1487
Tru9I TTAA 4 cut(s) 113, 227, 1409, 1487
TscAI CASTG 5 cut(s) 138, 217, 328, 1137, 1933
TseFI GTSAC 2 cut(s) 1322, 1611
TseI GCWGC 5 cut(s) 638, 919, 1877, 1880, 1918
Tsp45I GTSAC 2 cut(s) 1322, 1611
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 5 cut(s) 138, 217, 328, 1137, 1933
VpaK11BI GGWCC 1 cut(s) 1001
VspI ATTAAT 1 cut(s) 227
XapI RAATTY 5 cut(s) 83, 274, 284, 783, 1073
XceI RCATGY 4 cut(s) 1140, 1582, 1612, 1818
XmaJI CCTAGG 1 cut(s) 292
XspI CTAG 5 cut(s) 33, 293, 929, 2007, 2042
ZrmI AGTACT 1 cut(s) 432
Zsp2I ATGCAT 3 cut(s) 454, 955, 1801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.