RchiOBHm_Chr2g0166301

receptor-like protein kinase At1g67000

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000114
Physical Location & Seq
Reverse (-)
81016040 .. 81016693
654 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53418

Sequence Viewer

Length: 654 bp
ATGATGAACTCCCTTGTGCTTCAATCTTTGATTCCTTTCCTCGTGATCACTTCCTTTTTCTCAATCAATCTTCCATTCACTCTGTGTGCCGATGATCCTCAATATGCCTTCTGCAACCAGGCCATCAACTGTGGCAACATCCCTAACATCTCGTACCCCTTTTGGGGAGTGAATCGACCTAACTATTGTGGCCAGCCCGGGTTCGAAATCGAATGCCAAGACAATGTCCCAGTGATCAACATGAGAAATATCAACTTCAGAATTCTTGAGACGAACTCTAGTAGTACAGATCGGGCTGTTAAAGTTGCTAGGAACGATTATTGGAAAACTATCTGCCCCATTTCTTATGTTAACACCAGCATCGACTTTTCTCTGTTCAATTATGGTTCTGGGCTTCAGAACATGTCTTTTTACTACGGATGTAATACTACTTCAAATCCTACAGGGTTAAATGTTTCCCTTGTTTGCAACAGTAGTGTCACTGTCTCCTATCTCACACAATCACAAACTAGTGGACTCCCGTTTAGACCAGTTTCTACTGGGGCATGCGAAAATGAGGTTCTGGTTCCGGTTCCGAAACGGCTGTTTTGGCTCTTGATAACAATCAAACGACGATCCAAGAAGCCATCGATGGGGGTTTCGTACTGGGATTGA

Protein Analysis

217

Amino Acids

24.38

Weight (kDa)

8.36

Isoelectric Point (pI)

55.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 96 2.7e-16 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000114)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_4g01160 FvH4_6g34940 FvH4_6g44062 FvH4_6g44106 FvH4_6g44106 FvH4_6g44106 FvH4_6g44107 FvH4_6g44107 FvH4_6g44109 FvH4_6g44140 FvH4_6g44190 FvH4_6g44200 FvH4_6g44240 FvH4_6g44241 FvH4_6g44242
malus_domestica MD09G1094900.v1.1 MD09G1100300.v1.1 MD09G1100400.v1.1 MD09G1100600.v1.1 MD09G1100800.v1.1 MD09G1100900.v1.1 MD09G1101300.v1.1 MD09G1255200.v1.1 MD17G1088400.v1.1 MD17G1088600.v1.1 MD17G1247900.v1.1 MD17G1248300.v1.1
prunus_persica Prupe.1G497000_v2.0.a1 Prupe.2G033300_v2.0.a1 Prupe.3G145500_v2.0.a1 Prupe.3G225400_v2.0.a1 Prupe.3G225700_v2.0.a1 Prupe.3G225700_v2.0.a1
pyrus_communis pycom06g12720 pycom06g12740 pycom09g01960 pycom09g02450 pycom17g08520 pycom17g08530 pycom17g08560 pycom17g24950 pycom17g25030 pycom17g25110 pycom17g25260 pycom17g25290
rosa_chinensis RchiOBHm_Chr1g0326521 RchiOBHm_Chr1g0326551 RchiOBHm_Chr1g0326591 RchiOBHm_Chr1g0328741 RchiOBHm_Chr1g0329371 RchiOBHm_Chr2g0145911 RchiOBHm_Chr2g0160891 RchiOBHm_Chr2g0160921 RchiOBHm_Chr2g0161001 RchiOBHm_Chr2g0161011 RchiOBHm_Chr2g0161431 RchiOBHm_Chr2g0165121 RchiOBHm_Chr2g0166301 RchiOBHm_Chr4g0399551 RchiOBHm_Chr4g0405681 RchiOBHm_Chr5g0066741 RchiOBHm_Chr5g0066841 RchiOBHm_Chr6g0273021 RchiOBHm_Chr7g0225151
rosa_laevigata RLG00000001843 RLG00000008827 RLG00000014283 RLG00000014284 RLG00000020173 RLG00000021244 RLG00000021246 RLG00000021248 RLG00000021283 RLG00000023606 RLG00000029921 RLG00000030066 RLG00000030067 RLG00000033456
rosa_multiflora Rmu_co7958161.1_g000001 Rmu_co8440151.1_g000001 Rmu_co8454989.1_g000001 Rmu_sc0000129.1_g000018 Rmu_sc0000322.1_g000011 Rmu_sc0000322.1_g000045 Rmu_sc0000605.1_g000051 Rmu_sc0000955.1_g000015 Rmu_sc0000955.1_g000020 Rmu_sc0001188.1_g000017 Rmu_sc0001188.1_g000019 Rmu_sc0001355.1_g000015 Rmu_sc0001702.1_g000005 Rmu_sc0001702.1_g000006 Rmu_sc0001702.1_g000007 Rmu_sc0001702.1_g000008 Rmu_sc0001702.1_g000009 Rmu_sc0001702.1_g000010 Rmu_sc0001702.1_g000011 Rmu_sc0001702.1_g000012 Rmu_sc0001702.1_g000013 Rmu_sc0001702.1_g000014 Rmu_sc0001702.1_g000015 Rmu_sc0001702.1_g000016 Rmu_sc0002473.1_g000012 Rmu_sc0002473.1_g000015 Rmu_sc0002773.1_g000029 Rmu_sc0004178.1_g000002 Rmu_sc0005189.1_g000006 Rmu_sc0005860.1_g000004 Rmu_sc0009205.1_g000005 Rmu_sc0009205.1_g000007 Rmu_sc0009205.1_g000023 Rmu_sc0009472.1_g000005 Rmu_sc0009858.1_g000015 Rmu_sc0020187.1_g000001
rosa_roxburghii Rroxscaffold_176G00431460 Rroxscaffold_176G00431480 Rroxscaffold_1G00014330 Rroxscaffold_1G00031590 Rroxscaffold_2G00088820 Rroxscaffold_2G00089330 Rroxscaffold_2G00089360 Rroxscaffold_2G00089370 Rroxscaffold_2G00089380 Rroxscaffold_2G00100370 Rroxscaffold_2G00100380 Rroxscaffold_4G00321410 Rroxscaffold_4G00323700 Rroxscaffold_4G00327640 Rroxscaffold_4G00327650 Rroxscaffold_5G00344450 Rroxscaffold_7G00202770 Rroxscaffold_7G00203450 Rroxscaffold_7G00203540
rosa_rugosa Rorug01G0061100 Rorug02G0482100 Rorug02G0485900 Rorug02G0485900 Rorug02G0489500 Rorug02G0489600 Rorug02G0489700 Rorug04G0016600 Rorug04G0016700 Rorug05G0379000 Rorug05G0516700 Rorug06G0007900 Rorug06G0009300
rosa_samantha Rh1AG076700 Rh1AG076800 Rh1AG077000 Rh1AG077300 Rh1AG094400 Rh1AG097500 Rh1AG402000 Rh1BG062700 Rh1BG062900 Rh1CG075500 Rh1CG075600 Rh1CG075800 Rh1CG090900 Rh1CG091400 Rh1CG091600 Rh1CG094100 Rh1DG082300 Rh1DG082500 Rh1DG097400 Rh2AG448200 Rh2AG448300 Rh2AG511600 Rh2AG547500 Rh2AG551400 Rh2AG551700 Rh2AG555800 Rh2AG588000 Rh2BG565900 Rh2BG566000 Rh2BG566300 Rh2BG569000 Rh2BG600000 Rh2CG435200 Rh2CG435300 Rh2CG496600 Rh2CG535400 Rh2CG535600 Rh2CG539700 Rh2CG562700 Rh2CG570900 Rh2DG574600 Rh2DG574800 Rh2DG578600 Rh2DG610200 Rh4CG140900 Rh4DG127800 Rh5AG438500 Rh5BG436500 Rh5BG455500 Rh5CG477600 Rh5DG470300 Rh6AG129500 Rh6AG129900 Rh6BG125700 Rh6BG126000 Rh6BG126700 Rh6BG126800 Rh6CG124500 Rh6CG124800 Rh6CG125200 Rh6CG125300 Rh6DG111400 Rh6DG111700 Rh6DG111800 Rh7DG373300
rosa_wichuraiana Rw0G014010 Rw1G006140 Rw1G006160 Rw1G006180 Rw1G006190 Rw1G007340 Rw1G007360 Rw2G045660 Rw2G045670 Rw2G045970 Rw2G048220 Rw4G010820 Rw5G041010 Rw6G011220 Rw6G011230 Rw7G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 89, 609
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 261
AcuI CTGAAG 2 cut(s) 241, 380
AdeI CACNNNGTG 1 cut(s) 84
AfaI GTAC 3 cut(s) 155, 286, 644
AfiI CCNNNNNNNGG 3 cut(s) 163, 164, 632
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 3 cut(s) 23, 379, 435
AhlI ACTAGT 1 cut(s) 509
AjnI CCWGG 1 cut(s) 117
Alw26I GTCTC 2 cut(s) 263, 490
AlwI GGATC 2 cut(s) 89, 609
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 120, 190
ApoI RAATTY 1 cut(s) 261
AsuC2I CCSGG 2 cut(s) 198, 199
AsuII TTCGAA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 197
BalI TGGCCA 1 cut(s) 192
BarI GAAGNNNNNNTAC 2 cut(s) 415, 447
BauI CACGAG 1 cut(s) 41
BccI CCATC 3 cut(s) 131, 625, 634
BceAI ACGGC 1 cut(s) 596
BciT130I CCWGG 1 cut(s) 119
BclI TGATCA 2 cut(s) 45, 234
BcnI CCSGG 2 cut(s) 198, 199
BcoDI GTCTC 2 cut(s) 263, 490
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 3 cut(s) 279, 309, 510
BfmI CTRYAG 1 cut(s) 441
Bme1390I CCNGG 3 cut(s) 119, 198, 199
BmeT110I CYCGRG 1 cut(s) 197
BmiI GGNNCC 2 cut(s) 567, 573
BmrFI CCNGG 3 cut(s) 119, 198, 199
BmrI ACTGGG 2 cut(s) 224, 549
BmsI GCATC 1 cut(s) 369
BmuI ACTGGG 2 cut(s) 224, 549
BplI GAGNNNNNCTC 2 cut(s) 260, 292
Bpu14I TTCGAA 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 198, 199
Bsa29I ATCGAT 1 cut(s) 629
BsaBI GATNNNNATC 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 197
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 164, 632
Bse1I ACTGG 4 cut(s) 230, 530, 544, 650
Bse8I GATNNNNATC 1 cut(s) 602
BseBI CCWGG 1 cut(s) 119
BseCI ATCGAT 1 cut(s) 629
BseDI CCNNGG 1 cut(s) 197
BseGI GGATG 2 cut(s) 138, 425
BseJI GATNNNNATC 1 cut(s) 602
BseLI CCNNNNNNNGG 3 cut(s) 163, 164, 632
BseNI ACTGG 4 cut(s) 230, 530, 544, 650
BshFI GGCC 2 cut(s) 122, 192
BshVI ATCGAT 1 cut(s) 629
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 198, 569
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 163, 164, 632
BsmAI GTCTC 2 cut(s) 263, 490
BsmBI CGTCTC 1 cut(s) 263
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 218
BsnI GGCC 2 cut(s) 122, 192
BsoBI CYCGRG 1 cut(s) 197
Bsp119I TTCGAA 1 cut(s) 204
Bsp143I GATC 5 cut(s) 45, 94, 234, 289, 614
BspANI GGCC 2 cut(s) 122, 192
BspDI ATCGAT 1 cut(s) 629
BspLI GGNNCC 2 cut(s) 567, 573
BspPI GGATC 2 cut(s) 89, 609
BspT104I TTCGAA 1 cut(s) 204
BsrI ACTGG 4 cut(s) 230, 530, 544, 650
BssECI CCNNGG 1 cut(s) 197
BssMI GATC 5 cut(s) 45, 94, 234, 289, 614
BssSI CACGAG 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 119
Bst4CI ACNGT 3 cut(s) 131, 473, 484
BstBI TTCGAA 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 194, 547
BstF5I GGATG 2 cut(s) 138, 425
BstKTI GATC 5 cut(s) 48, 97, 237, 292, 617
BstMAI GTCTC 2 cut(s) 263, 490
BstMBI GATC 5 cut(s) 45, 94, 234, 289, 614
BstMWI GCNNNNNNNGC 1 cut(s) 589
BstNI CCWGG 1 cut(s) 119
BstNSI RCATGY 2 cut(s) 406, 549
BstSCI CCNGG 3 cut(s) 117, 196, 197
BstSFI CTRYAG 1 cut(s) 441
Bsu15I ATCGAT 1 cut(s) 629
BsuRI GGCC 2 cut(s) 122, 192
BsuTUI ATCGAT 1 cut(s) 629
BtsCI GGATG 2 cut(s) 138, 425
BtsIMutI CAGTG 2 cut(s) 237, 480
Cac8I GCNNGC 2 cut(s) 194, 547
Cfr9I CCCGGG 1 cut(s) 197
ClaI ATCGAT 1 cut(s) 629
Csp6I GTAC 3 cut(s) 154, 285, 643
CviAII CATG 3 cut(s) 241, 403, 546
CviJI RGCY 8 cut(s) 122, 192, 196, 296, 394, 583, 592, 625
CviKI_1 RGCY 8 cut(s) 122, 192, 196, 296, 394, 583, 592, 625
CviQI GTAC 3 cut(s) 154, 285, 643
DpnI GATC 5 cut(s) 47, 96, 236, 291, 616
DpnII GATC 5 cut(s) 45, 94, 234, 289, 614
DraIII CACNNNGTG 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 190
Eco57I CTGAAG 2 cut(s) 241, 380
Eco88I CYCGRG 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 117
Esp3I CGTCTC 1 cut(s) 263
FaeI CATG 3 cut(s) 244, 406, 549
FaiI YATR 6 cut(s) 105, 242, 348, 384, 404, 547
FaqI GGGAC 1 cut(s) 212
FatI CATG 3 cut(s) 240, 402, 545
FbaI TGATCA 2 cut(s) 45, 234
FokI GGATG 2 cut(s) 125, 432
FspBI CTAG 3 cut(s) 279, 309, 510
HaeIII GGCC 2 cut(s) 122, 192
HapII CCGG 2 cut(s) 198, 569
Hin1II CATG 3 cut(s) 244, 406, 549
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HinfI GANTC 3 cut(s) 31, 172, 516
HpaI GTTAAC 1 cut(s) 352
HpaII CCGG 2 cut(s) 198, 569
Hpy166II GTNNAC 2 cut(s) 352, 515
Hpy188I TCNGA 3 cut(s) 260, 399, 576
Hpy188III TCNNGA 3 cut(s) 43, 266, 595
Hpy8I GTNNAC 2 cut(s) 352, 515
Hpy99I CGWCG 1 cut(s) 615
HpyAV CCTTC 1 cut(s) 118
HpyCH4III ACNGT 3 cut(s) 131, 473, 484
HpyCH4V TGCA 2 cut(s) 114, 468
HpyF10VI GCNNNNNNNGC 1 cut(s) 589
Hsp92II CATG 3 cut(s) 244, 406, 549
Ksp22I TGATCA 2 cut(s) 45, 234
KspAI GTTAAC 1 cut(s) 352
Kzo9I GATC 5 cut(s) 45, 94, 234, 289, 614
LweI GCATC 1 cut(s) 369
MaeI CTAG 3 cut(s) 279, 309, 510
MaeIII GTNAC 1 cut(s) 478
MalI GATC 5 cut(s) 47, 96, 236, 291, 616
MboI GATC 5 cut(s) 45, 94, 234, 289, 614
MboII GAAGA 1 cut(s) 62
MlsI TGGCCA 1 cut(s) 192
MluCI AATT 2 cut(s) 261, 379
MluNI TGGCCA 1 cut(s) 192
MlyI GAGTC 1 cut(s) 510
MnlI CCTC 3 cut(s) 50, 108, 550
Mox20I TGGCCA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 192
MseI TTAA 3 cut(s) 300, 351, 449
Msp20I TGGCCA 1 cut(s) 192
MspI CCGG 2 cut(s) 198, 569
MspR9I CCNGG 3 cut(s) 119, 198, 199
Mva1269I GAATGC 1 cut(s) 218
MvaI CCWGG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 589
NciI CCSGG 2 cut(s) 198, 199
NdeII GATC 5 cut(s) 45, 94, 234, 289, 614
NlaIII CATG 3 cut(s) 244, 406, 549
NlaIV GGNNCC 2 cut(s) 567, 573
NmuCI GTSAC 1 cut(s) 478
NspI RCATGY 2 cut(s) 406, 549
NspV TTCGAA 1 cut(s) 204
PaeI GCATGC 1 cut(s) 549
PciI ACATGT 1 cut(s) 402
PctI GAATGC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 31, 172
PflFI GACNNNGTC 1 cut(s) 224
PleI GAGTC 1 cut(s) 510
PpsI GAGTC 1 cut(s) 510
PscI ACATGT 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 567, 573
PsyI GACNNNGTC 1 cut(s) 224
RsaI GTAC 3 cut(s) 155, 286, 644
RsaNI GTAC 3 cut(s) 154, 285, 643
SaqAI TTAA 3 cut(s) 300, 351, 449
Sau3AI GATC 5 cut(s) 45, 94, 234, 289, 614
SchI GAGTC 1 cut(s) 510
ScrFI CCNGG 3 cut(s) 119, 198, 199
SetI ASST 2 cut(s) 181, 561
SfaNI GCATC 1 cut(s) 369
SfcI CTRYAG 1 cut(s) 441
SfuI TTCGAA 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SpeI ACTAGT 1 cut(s) 509
SphI GCATGC 1 cut(s) 549
Sse9I AATT 2 cut(s) 261, 379
SspMI CTAG 3 cut(s) 279, 309, 510
StyD4I CCNGG 3 cut(s) 117, 196, 197
TaaI ACNGT 3 cut(s) 131, 473, 484
TaqI TCGA 5 cut(s) 175, 204, 210, 363, 629
TasI AATT 2 cut(s) 261, 379
TatI WGTACW 1 cut(s) 284
TfiI GAWTC 2 cut(s) 31, 172
Tru1I TTAA 3 cut(s) 300, 351, 449
Tru9I TTAA 3 cut(s) 300, 351, 449
TscAI CASTG 2 cut(s) 237, 487
TseFI GTSAC 1 cut(s) 478
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 1 cut(s) 20
TspGWI ACGGA 1 cut(s) 432
TspMI CCCGGG 1 cut(s) 197
TspRI CASTG 2 cut(s) 237, 487
Tth111I GACNNNGTC 1 cut(s) 224
XapI RAATTY 1 cut(s) 261
XceI RCATGY 2 cut(s) 406, 549
XmaI CCCGGG 1 cut(s) 197
XspI CTAG 3 cut(s) 279, 309, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.