MD17G1248300.v1.1

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000114
Physical Location & Seq
Reverse (-)
29707870 .. 29708742
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1248300.v1.1.491

Sequence Viewer

Length: 873 bp
ATGCATTTCTTTCTGCTCCAATCGTTGTTTCCATTTCTTTTGATCGCTTCCTTCCTTTCGATCAACGTCGAGTTAAGTCTATGCCAGAATCAACAATTCACAAACTGCAGCAGCAATATCACTTGTGGTAGCGTCGGAGGCATATCCTATCCCTTTTGGGGGGTAAACCGAGCTAGTTACTGTGGTCAACCCGGATTTGAGGTCCAATGCCTAAACAACTTCCCTGTGTTCAACATGAAGGGTGCGAGCTATAGAATTCTCCAAATGAACACTACAAATTCTCGGACCGTTAAGGTCGCTAGGAACGATTACTGGGGTACTATTTGTCCTCAGACTTTTGTCAACACCACCCTCAACTTCTCTCTGTTTGATTATGTTTCTGGTTATAGAAACATGACGTTTTACTACCAATGCTTTGCCGCCATAACGCTTCCTAGTCCGCAGTCCTGCAACATAAGCAATACAGTGAACCCTGTTTACTATGTGACACAACCGGTGACAGTTGGTCAGGCATATTCTGTGAGCTGTACACATGAGGTTATTGTTCCCGTTTATGAAGAAGCTGCTCTTGCTATAGAGGCCGCCACTGTGCGCAGTCAAACAATTGTCACCGATGCGGTAGAGGGGGGTTTCTCACTGCAATTGGAGATTGACAACGATGAATGCGACAAATGTTTGGGATCAGGTGGACAGTGTGGGCTTGACAATACTACTAATTCTGGATTCCGTTGCTTCTGCGCAGATCGAGCCTATGCATCCACATGTAATGGAACTAGTACTTCAAGTCAAGGCGGAGGTACGTCTGTCCCTTGTCATTCCCTTATAAATTACACATATGGAACTCCTAGCTTGCTCTTTGTTTTAGATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase WAK_assoc

Protein Analysis

291

Amino Acids

31.68

Weight (kDa)

4.74

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 36 - 101 7e-14 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 159 - 249 1.1e-14 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000114)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_4g01160 FvH4_6g34940 FvH4_6g44062 FvH4_6g44106 FvH4_6g44106 FvH4_6g44106 FvH4_6g44107 FvH4_6g44107 FvH4_6g44109 FvH4_6g44140 FvH4_6g44190 FvH4_6g44200 FvH4_6g44240 FvH4_6g44241 FvH4_6g44242
malus_domestica MD09G1094900.v1.1 MD09G1100300.v1.1 MD09G1100400.v1.1 MD09G1100600.v1.1 MD09G1100800.v1.1 MD09G1100900.v1.1 MD09G1101300.v1.1 MD09G1255200.v1.1 MD17G1088400.v1.1 MD17G1088600.v1.1 MD17G1247900.v1.1 MD17G1248300.v1.1
prunus_persica Prupe.1G497000_v2.0.a1 Prupe.2G033300_v2.0.a1 Prupe.3G145500_v2.0.a1 Prupe.3G225400_v2.0.a1 Prupe.3G225700_v2.0.a1 Prupe.3G225700_v2.0.a1
pyrus_communis pycom06g12720 pycom06g12740 pycom09g01960 pycom09g02450 pycom17g08520 pycom17g08530 pycom17g08560 pycom17g24950 pycom17g25030 pycom17g25110 pycom17g25260 pycom17g25290
rosa_chinensis RchiOBHm_Chr1g0326521 RchiOBHm_Chr1g0326551 RchiOBHm_Chr1g0326591 RchiOBHm_Chr1g0328741 RchiOBHm_Chr1g0329371 RchiOBHm_Chr2g0145911 RchiOBHm_Chr2g0160891 RchiOBHm_Chr2g0160921 RchiOBHm_Chr2g0161001 RchiOBHm_Chr2g0161011 RchiOBHm_Chr2g0161431 RchiOBHm_Chr2g0165121 RchiOBHm_Chr2g0166301 RchiOBHm_Chr4g0399551 RchiOBHm_Chr4g0405681 RchiOBHm_Chr5g0066741 RchiOBHm_Chr5g0066841 RchiOBHm_Chr6g0273021 RchiOBHm_Chr7g0225151
rosa_laevigata RLG00000001843 RLG00000008827 RLG00000014283 RLG00000014284 RLG00000020173 RLG00000021244 RLG00000021246 RLG00000021248 RLG00000021283 RLG00000023606 RLG00000029921 RLG00000030066 RLG00000030067 RLG00000033456
rosa_multiflora Rmu_co7958161.1_g000001 Rmu_co8440151.1_g000001 Rmu_co8454989.1_g000001 Rmu_sc0000129.1_g000018 Rmu_sc0000322.1_g000011 Rmu_sc0000322.1_g000045 Rmu_sc0000605.1_g000051 Rmu_sc0000955.1_g000015 Rmu_sc0000955.1_g000020 Rmu_sc0001188.1_g000017 Rmu_sc0001188.1_g000019 Rmu_sc0001355.1_g000015 Rmu_sc0001702.1_g000005 Rmu_sc0001702.1_g000006 Rmu_sc0001702.1_g000007 Rmu_sc0001702.1_g000008 Rmu_sc0001702.1_g000009 Rmu_sc0001702.1_g000010 Rmu_sc0001702.1_g000011 Rmu_sc0001702.1_g000012 Rmu_sc0001702.1_g000013 Rmu_sc0001702.1_g000014 Rmu_sc0001702.1_g000015 Rmu_sc0001702.1_g000016 Rmu_sc0002473.1_g000012 Rmu_sc0002473.1_g000015 Rmu_sc0002773.1_g000029 Rmu_sc0004178.1_g000002 Rmu_sc0005189.1_g000006 Rmu_sc0005860.1_g000004 Rmu_sc0009205.1_g000005 Rmu_sc0009205.1_g000007 Rmu_sc0009205.1_g000023 Rmu_sc0009472.1_g000005 Rmu_sc0009858.1_g000015 Rmu_sc0020187.1_g000001
rosa_roxburghii Rroxscaffold_176G00431460 Rroxscaffold_176G00431480 Rroxscaffold_1G00014330 Rroxscaffold_1G00031590 Rroxscaffold_2G00088820 Rroxscaffold_2G00089330 Rroxscaffold_2G00089360 Rroxscaffold_2G00089370 Rroxscaffold_2G00089380 Rroxscaffold_2G00100370 Rroxscaffold_2G00100380 Rroxscaffold_4G00321410 Rroxscaffold_4G00323700 Rroxscaffold_4G00327640 Rroxscaffold_4G00327650 Rroxscaffold_5G00344450 Rroxscaffold_7G00202770 Rroxscaffold_7G00203450 Rroxscaffold_7G00203540
rosa_rugosa Rorug01G0061100 Rorug02G0482100 Rorug02G0485900 Rorug02G0485900 Rorug02G0489500 Rorug02G0489600 Rorug02G0489700 Rorug04G0016600 Rorug04G0016700 Rorug05G0379000 Rorug05G0516700 Rorug06G0007900 Rorug06G0009300
rosa_samantha Rh1AG076700 Rh1AG076800 Rh1AG077000 Rh1AG077300 Rh1AG094400 Rh1AG097500 Rh1AG402000 Rh1BG062700 Rh1BG062900 Rh1CG075500 Rh1CG075600 Rh1CG075800 Rh1CG090900 Rh1CG091400 Rh1CG091600 Rh1CG094100 Rh1DG082300 Rh1DG082500 Rh1DG097400 Rh2AG448200 Rh2AG448300 Rh2AG511600 Rh2AG547500 Rh2AG551400 Rh2AG551700 Rh2AG555800 Rh2AG588000 Rh2BG565900 Rh2BG566000 Rh2BG566300 Rh2BG569000 Rh2BG600000 Rh2CG435200 Rh2CG435300 Rh2CG496600 Rh2CG535400 Rh2CG535600 Rh2CG539700 Rh2CG562700 Rh2CG570900 Rh2DG574600 Rh2DG574800 Rh2DG578600 Rh2DG610200 Rh4CG140900 Rh4DG127800 Rh5AG438500 Rh5BG436500 Rh5BG455500 Rh5CG477600 Rh5DG470300 Rh6AG129500 Rh6AG129900 Rh6BG125700 Rh6BG126000 Rh6BG126700 Rh6BG126800 Rh6CG124500 Rh6CG124800 Rh6CG125200 Rh6CG125300 Rh6DG111400 Rh6DG111700 Rh6DG111800 Rh7DG373300
rosa_wichuraiana Rw0G014010 Rw1G006140 Rw1G006160 Rw1G006180 Rw1G006190 Rw1G007340 Rw1G007360 Rw2G045660 Rw2G045670 Rw2G045970 Rw2G048220 Rw4G010820 Rw5G041010 Rw6G011220 Rw6G011230 Rw7G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
Acc16I TGCGCA 2 cut(s) 593, 739
AciI CCGC 5 cut(s) 420, 440, 582, 617, 792
AclWI GGATC 1 cut(s) 688
AcsI RAATTY 2 cut(s) 255, 277
AfaI GTAC 4 cut(s) 319, 529, 778, 799
AfiI CCNNNNNNNGG 2 cut(s) 158, 159
AflIII ACRYGT 1 cut(s) 761
AgeI ACCGGT 1 cut(s) 493
AgsI TTSAA 2 cut(s) 232, 783
AhdI GACNNNNNGTC 1 cut(s) 504
AhlI ACTAGT 1 cut(s) 773
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AluBI AGCT 5 cut(s) 173, 249, 525, 563, 849
AluI AGCT 5 cut(s) 173, 249, 525, 563, 849
AlwI GGATC 1 cut(s) 688
AoxI GGCC 1 cut(s) 579
ApeKI GCWGC 3 cut(s) 108, 111, 563
ApoI RAATTY 2 cut(s) 255, 277
AsiGI ACCGGT 1 cut(s) 493
AspLEI GCGC 2 cut(s) 594, 740
AspS9I GGNCC 2 cut(s) 202, 285
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 2 cut(s) 508, 601
AvaII GGWCC 2 cut(s) 202, 285
BaeI ACNNNNGTAYC 2 cut(s) 789, 822
BbvI GCAGC 3 cut(s) 120, 123, 550
BcnI CCSGG 1 cut(s) 192
BcuI ACTAGT 1 cut(s) 773
BfaI CTAG 5 cut(s) 174, 300, 435, 774, 846
BfmI CTRYAG 3 cut(s) 106, 250, 573
BisI GCNGC 5 cut(s) 109, 112, 420, 564, 582
BlsI GCNGC 5 cut(s) 110, 113, 421, 565, 583
BmcAI AGTACT 1 cut(s) 778
Bme1390I CCNGG 1 cut(s) 192
Bme18I GGWCC 2 cut(s) 202, 285
BmeRI GACNNNNNGTC 1 cut(s) 504
BmgT120I GGNCC 2 cut(s) 202, 285
BmrFI CCNGG 1 cut(s) 192
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 2 cut(s) 604, 764
BmuI ACTGGG 1 cut(s) 322
BoxI GACNNNNGTC 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 493
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 159
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 1 cut(s) 317
BseGI GGATG 1 cut(s) 755
BseLI CCNNNNNNNGG 2 cut(s) 158, 159
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 1 cut(s) 317
BseXI GCAGC 3 cut(s) 120, 123, 550
BshFI GGCC 1 cut(s) 581
BshTI ACCGGT 1 cut(s) 493
BsiSI CCGG 2 cut(s) 192, 494
BslFI GGGAC 1 cut(s) 791
BslI CCNNNNNNNGG 2 cut(s) 158, 159
BsmFI GGGAC 1 cut(s) 791
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 1 cut(s) 581
Bsp1407I TGTACA 1 cut(s) 527
Bsp143I GATC 4 cut(s) 42, 60, 680, 742
BspACI CCGC 5 cut(s) 420, 440, 582, 617, 792
BspANI GGCC 1 cut(s) 581
BspCNI CTCAG 1 cut(s) 343
BspMAI CTGCAG 1 cut(s) 110
BspPI GGATC 1 cut(s) 688
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 527
BsrI ACTGG 1 cut(s) 317
BssAI RCCGGY 1 cut(s) 493
BssMI GATC 4 cut(s) 42, 60, 680, 742
Bst4CI ACNGT 6 cut(s) 182, 289, 466, 502, 589, 693
BstAUI TGTACA 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 247, 851
BstDEI CTNAG 1 cut(s) 330
BstF5I GGATG 1 cut(s) 755
BstHHI GCGC 2 cut(s) 594, 740
BstKTI GATC 4 cut(s) 45, 63, 683, 745
BstMBI GATC 4 cut(s) 42, 60, 680, 742
BstMWI GCNNNNNNNGC 5 cut(s) 138, 456, 569, 578, 746
BstNSI RCATGY 1 cut(s) 765
BstPAI GACNNNNGTC 1 cut(s) 338
BstSCI CCNGG 1 cut(s) 190
BstSFI CTRYAG 3 cut(s) 106, 250, 573
BstV1I GCAGC 3 cut(s) 120, 123, 550
BsuRI GGCC 1 cut(s) 581
BtsCI GGATG 1 cut(s) 755
BtsI GCAGTG 1 cut(s) 635
BtsIMutI CAGTG 4 cut(s) 471, 585, 635, 698
Cac8I GCNNGC 2 cut(s) 247, 851
CfoI GCGC 2 cut(s) 594, 740
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 2 cut(s) 202, 285
CpoI CGGWCCG 1 cut(s) 285
CseI GACGC 1 cut(s) 121
Csp6I GTAC 4 cut(s) 318, 528, 777, 798
CspAI ACCGGT 1 cut(s) 493
CspI CGGWCCG 1 cut(s) 285
CviAII CATG 4 cut(s) 235, 394, 533, 762
CviJI RGCY 8 cut(s) 173, 249, 525, 563, 581, 700, 749, 849
CviKI_1 RGCY 8 cut(s) 173, 249, 525, 563, 581, 700, 749, 849
CviQI GTAC 4 cut(s) 318, 528, 777, 798
DdeI CTNAG 1 cut(s) 330
DpnI GATC 4 cut(s) 44, 62, 682, 744
DpnII GATC 4 cut(s) 42, 60, 680, 742
DriI GACNNNNNGTC 1 cut(s) 504
Eam1105I GACNNNNNGTC 1 cut(s) 504
EciI GGCGGA 1 cut(s) 807
Eco47I GGWCC 2 cut(s) 202, 285
EcoRI GAATTC 1 cut(s) 255
EcoT22I ATGCAT 2 cut(s) 6, 757
FaeI CATG 4 cut(s) 238, 397, 536, 765
FalI AAGNNNNNCTT 2 cut(s) 552, 584
FaqI GGGAC 1 cut(s) 791
FatI CATG 4 cut(s) 234, 393, 532, 761
FauNDI CATATG 1 cut(s) 835
Fnu4HI GCNGC 5 cut(s) 109, 112, 420, 564, 582
FokI GGATG 1 cut(s) 742
Fsp4HI GCNGC 5 cut(s) 109, 112, 420, 564, 582
FspBI CTAG 5 cut(s) 174, 300, 435, 774, 846
FspI TGCGCA 2 cut(s) 593, 739
GlaI GCGC 2 cut(s) 593, 739
GluI GCNGC 5 cut(s) 109, 112, 420, 564, 582
HaeIII GGCC 1 cut(s) 581
HapII CCGG 2 cut(s) 192, 494
HgaI GACGC 1 cut(s) 121
HhaI GCGC 2 cut(s) 594, 740
Hin1II CATG 4 cut(s) 238, 397, 536, 765
Hin6I GCGC 2 cut(s) 592, 738
HinP1I GCGC 2 cut(s) 592, 738
HincII GTYRAC 2 cut(s) 188, 343
HindII GTYRAC 2 cut(s) 188, 343
HinfI GANTC 2 cut(s) 88, 723
HpaII CCGG 2 cut(s) 192, 494
HphI GGTGA 2 cut(s) 508, 601
Hpy166II GTNNAC 7 cut(s) 166, 188, 343, 469, 478, 530, 689
Hpy188I TCNGA 3 cut(s) 137, 285, 333
Hpy188III TCNNGA 1 cut(s) 720
Hpy8I GTNNAC 7 cut(s) 166, 188, 343, 469, 478, 530, 689
Hpy99I CGWCG 2 cut(s) 71, 137
HpyAV CCTTC 2 cut(s) 61, 232
HpyCH4III ACNGT 6 cut(s) 182, 289, 466, 502, 589, 693
HpyCH4IV ACGT 3 cut(s) 66, 398, 800
HpyCH4V TGCA 5 cut(s) 4, 108, 450, 640, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 456, 569, 578, 746
HpyF3I CTNAG 1 cut(s) 330
HpySE526I ACGT 3 cut(s) 66, 398, 800
Hsp92II CATG 4 cut(s) 238, 397, 536, 765
HspAI GCGC 2 cut(s) 592, 738
Kzo9I GATC 4 cut(s) 42, 60, 680, 742
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 3 cut(s) 120, 123, 550
LweI GCATC 2 cut(s) 604, 764
MaeI CTAG 5 cut(s) 174, 300, 435, 774, 846
MaeII ACGT 3 cut(s) 66, 398, 800
MaeIII GTNAC 4 cut(s) 176, 484, 496, 607
MalI GATC 4 cut(s) 44, 62, 682, 744
MboI GATC 4 cut(s) 42, 60, 680, 742
MboII GAAGA 1 cut(s) 569
MfeI CAATTG 2 cut(s) 603, 641
MluCI AATT 7 cut(s) 95, 255, 277, 603, 641, 715, 826
MmeI TCCRAC 1 cut(s) 115
MnlI CCTC 8 cut(s) 131, 193, 339, 362, 529, 571, 616, 788
Mph1103I ATGCAT 2 cut(s) 6, 757
MseI TTAA 2 cut(s) 74, 291
MslI CAYNNNNRTG 1 cut(s) 760
MspI CCGG 2 cut(s) 192, 494
MspR9I CCNGG 1 cut(s) 192
MunI CAATTG 2 cut(s) 603, 641
Mva1269I GAATGC 1 cut(s) 668
MwoI GCNNNNNNNGC 5 cut(s) 138, 456, 569, 578, 746
NciI CCSGG 1 cut(s) 192
NdeI CATATG 1 cut(s) 835
NdeII GATC 4 cut(s) 42, 60, 680, 742
NlaIII CATG 4 cut(s) 238, 397, 536, 765
NmuCI GTSAC 3 cut(s) 484, 496, 607
NsbI TGCGCA 2 cut(s) 593, 739
NsiI ATGCAT 2 cut(s) 6, 757
NspI RCATGY 1 cut(s) 765
PciI ACATGT 1 cut(s) 761
PcsI WCGNNNNNNNCGW 2 cut(s) 303, 663
PctI GAATGC 1 cut(s) 668
PfeI GAWTC 2 cut(s) 88, 723
PinAI ACCGGT 1 cut(s) 493
PkrI GCNGC 5 cut(s) 110, 113, 421, 565, 583
PscI ACATGT 1 cut(s) 761
PshAI GACNNNNGTC 1 cut(s) 338
PsiI TTATAA 1 cut(s) 824
PspPI GGNCC 2 cut(s) 202, 285
PsrI GAACNNNNNNTAC 2 cut(s) 461, 493
PstI CTGCAG 1 cut(s) 110
RsaI GTAC 4 cut(s) 319, 529, 778, 799
RsaNI GTAC 4 cut(s) 318, 528, 777, 798
RseI CAYNNNNRTG 1 cut(s) 760
Rsr2I CGGWCCG 1 cut(s) 285
RsrII CGGWCCG 1 cut(s) 285
SaqAI TTAA 2 cut(s) 74, 291
SatI GCNGC 5 cut(s) 109, 112, 420, 564, 582
Sau3AI GATC 4 cut(s) 42, 60, 680, 742
Sau96I GGNCC 2 cut(s) 202, 285
ScaI AGTACT 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 192
SfaNI GCATC 2 cut(s) 604, 764
SfcI CTRYAG 3 cut(s) 106, 250, 573
SinI GGWCC 2 cut(s) 202, 285
SmiMI CAYNNNNRTG 1 cut(s) 760
SpeI ACTAGT 1 cut(s) 773
Sse9I AATT 7 cut(s) 95, 255, 277, 603, 641, 715, 826
SsiI CCGC 5 cut(s) 420, 440, 582, 617, 792
SspMI CTAG 5 cut(s) 174, 300, 435, 774, 846
StyD4I CCNGG 1 cut(s) 190
TaaI ACNGT 6 cut(s) 182, 289, 466, 502, 589, 693
TaiI ACGT 3 cut(s) 69, 401, 803
TaqI TCGA 3 cut(s) 59, 69, 745
TasI AATT 7 cut(s) 95, 255, 277, 603, 641, 715, 826
TatI WGTACW 2 cut(s) 527, 776
TauI GCSGC 2 cut(s) 422, 584
TfiI GAWTC 2 cut(s) 88, 723
Tru1I TTAA 2 cut(s) 74, 291
Tru9I TTAA 2 cut(s) 74, 291
TscAI CASTG 4 cut(s) 471, 592, 642, 698
TseFI GTSAC 3 cut(s) 484, 496, 607
TseI GCWGC 3 cut(s) 108, 111, 563
Tsp45I GTSAC 3 cut(s) 484, 496, 607
TspDTI ATGAA 4 cut(s) 251, 281, 570, 675
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 4 cut(s) 471, 592, 642, 698
VpaK11BI GGWCC 2 cut(s) 202, 285
XapI RAATTY 2 cut(s) 255, 277
XceI RCATGY 1 cut(s) 765
XspI CTAG 5 cut(s) 174, 300, 435, 774, 846
ZrmI AGTACT 1 cut(s) 778
Zsp2I ATGCAT 2 cut(s) 6, 757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.