MD17G1247900.v1.1

protein serine/threonine kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000114
Physical Location & Seq
Reverse (-)
29687834 .. 29688706
873 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1247900.v1.1.491

Sequence Viewer

Length: 873 bp
ATGCATTCCTTTCTGCTCCAATCGTTGTTTCCATTTCTTTTGATCGCTTCCTTCCTTTCGATCAACGTTGAGTTAAGTCTATGCCAGGATGAGGCACAATTCACAAACTGCAGCAGCGCTATCAATTGTGGTGGCATCGAAAACATCTCCTACCCCTTTTATGGGGTAAACCGAGCTAGTTACTGTGGTCAGCCAGGGTACGAGGTCCAATGTCTAAACGAAGTCCCTGTCATCAACGTAAGAGGTCTGAGCTTTAGAATTCTTGAAATGAATTCTACTACTACTCCTCGGACTGTTAAGGTTGCTAGGCAGGATTACTGGGGTACTATCTGTCCTCCGACTTTCGTCAACACCACCCTCAACTTCTCTCTGTTTGATTATGGCTCTGGTTATACAAACATGACGTTTTACTACCAATGCAATGTCAACAACATAGGGCTTCCTAGCACGCAGTGCAACATAAGCAATACATTGAGCCCTGTTTTCTCTGTGCCAAGTTGGATGGTTGAGCAGATATCTTCTGGCGGCTGTAGAGATGAGGTTATTGTTCCGGTTTATACAGCAGCTGCTCAGGCTTTTGTGGCCAGTCAAACAACCGTCAGCGATGCGGTAAAAGGGGGCTTCCCATTGGAATTGCAGATTGACACCGATCAATGCAACAAATGTGAGGAATCAGGAGGACGGTGTGGGCTTAATACTAATTCTGGATTCAGCTGCTTTTGCCGAGATCAGGCCTATGCAACCATATGTAATGGAACTAGTAATTCATCTCAACCTGGAGGCGGAGGCGGAGGCGGAGGCGGAAGCGGAAGCGGAGGTACGTCTGTCCCTTGTCATTCTCTAGTAAATTACACATTAGGAACTCCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase WAK_assoc

Protein Analysis

291

Amino Acids

30.89

Weight (kDa)

4.63

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 37 - 102 4.5e-12 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 172 - 244 6.4e-14 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000114)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_1g07120 FvH4_4g01160 FvH4_6g34940 FvH4_6g44062 FvH4_6g44106 FvH4_6g44106 FvH4_6g44106 FvH4_6g44107 FvH4_6g44107 FvH4_6g44109 FvH4_6g44140 FvH4_6g44190 FvH4_6g44200 FvH4_6g44240 FvH4_6g44241 FvH4_6g44242
malus_domestica MD09G1094900.v1.1 MD09G1100300.v1.1 MD09G1100400.v1.1 MD09G1100600.v1.1 MD09G1100800.v1.1 MD09G1100900.v1.1 MD09G1101300.v1.1 MD09G1255200.v1.1 MD17G1088400.v1.1 MD17G1088600.v1.1 MD17G1247900.v1.1 MD17G1248300.v1.1
prunus_persica Prupe.1G497000_v2.0.a1 Prupe.2G033300_v2.0.a1 Prupe.3G145500_v2.0.a1 Prupe.3G225400_v2.0.a1 Prupe.3G225700_v2.0.a1 Prupe.3G225700_v2.0.a1
pyrus_communis pycom06g12720 pycom06g12740 pycom09g01960 pycom09g02450 pycom17g08520 pycom17g08530 pycom17g08560 pycom17g24950 pycom17g25030 pycom17g25110 pycom17g25260 pycom17g25290
rosa_chinensis RchiOBHm_Chr1g0326521 RchiOBHm_Chr1g0326551 RchiOBHm_Chr1g0326591 RchiOBHm_Chr1g0328741 RchiOBHm_Chr1g0329371 RchiOBHm_Chr2g0145911 RchiOBHm_Chr2g0160891 RchiOBHm_Chr2g0160921 RchiOBHm_Chr2g0161001 RchiOBHm_Chr2g0161011 RchiOBHm_Chr2g0161431 RchiOBHm_Chr2g0165121 RchiOBHm_Chr2g0166301 RchiOBHm_Chr4g0399551 RchiOBHm_Chr4g0405681 RchiOBHm_Chr5g0066741 RchiOBHm_Chr5g0066841 RchiOBHm_Chr6g0273021 RchiOBHm_Chr7g0225151
rosa_laevigata RLG00000001843 RLG00000008827 RLG00000014283 RLG00000014284 RLG00000020173 RLG00000021244 RLG00000021246 RLG00000021248 RLG00000021283 RLG00000023606 RLG00000029921 RLG00000030066 RLG00000030067 RLG00000033456
rosa_multiflora Rmu_co7958161.1_g000001 Rmu_co8440151.1_g000001 Rmu_co8454989.1_g000001 Rmu_sc0000129.1_g000018 Rmu_sc0000322.1_g000011 Rmu_sc0000322.1_g000045 Rmu_sc0000605.1_g000051 Rmu_sc0000955.1_g000015 Rmu_sc0000955.1_g000020 Rmu_sc0001188.1_g000017 Rmu_sc0001188.1_g000019 Rmu_sc0001355.1_g000015 Rmu_sc0001702.1_g000005 Rmu_sc0001702.1_g000006 Rmu_sc0001702.1_g000007 Rmu_sc0001702.1_g000008 Rmu_sc0001702.1_g000009 Rmu_sc0001702.1_g000010 Rmu_sc0001702.1_g000011 Rmu_sc0001702.1_g000012 Rmu_sc0001702.1_g000013 Rmu_sc0001702.1_g000014 Rmu_sc0001702.1_g000015 Rmu_sc0001702.1_g000016 Rmu_sc0002473.1_g000012 Rmu_sc0002473.1_g000015 Rmu_sc0002773.1_g000029 Rmu_sc0004178.1_g000002 Rmu_sc0005189.1_g000006 Rmu_sc0005860.1_g000004 Rmu_sc0009205.1_g000005 Rmu_sc0009205.1_g000007 Rmu_sc0009205.1_g000023 Rmu_sc0009472.1_g000005 Rmu_sc0009858.1_g000015 Rmu_sc0020187.1_g000001
rosa_roxburghii Rroxscaffold_176G00431460 Rroxscaffold_176G00431480 Rroxscaffold_1G00014330 Rroxscaffold_1G00031590 Rroxscaffold_2G00088820 Rroxscaffold_2G00089330 Rroxscaffold_2G00089360 Rroxscaffold_2G00089370 Rroxscaffold_2G00089380 Rroxscaffold_2G00100370 Rroxscaffold_2G00100380 Rroxscaffold_4G00321410 Rroxscaffold_4G00323700 Rroxscaffold_4G00327640 Rroxscaffold_4G00327650 Rroxscaffold_5G00344450 Rroxscaffold_7G00202770 Rroxscaffold_7G00203450 Rroxscaffold_7G00203540
rosa_rugosa Rorug01G0061100 Rorug02G0482100 Rorug02G0485900 Rorug02G0485900 Rorug02G0489500 Rorug02G0489600 Rorug02G0489700 Rorug04G0016600 Rorug04G0016700 Rorug05G0379000 Rorug05G0516700 Rorug06G0007900 Rorug06G0009300
rosa_samantha Rh1AG076700 Rh1AG076800 Rh1AG077000 Rh1AG077300 Rh1AG094400 Rh1AG097500 Rh1AG402000 Rh1BG062700 Rh1BG062900 Rh1CG075500 Rh1CG075600 Rh1CG075800 Rh1CG090900 Rh1CG091400 Rh1CG091600 Rh1CG094100 Rh1DG082300 Rh1DG082500 Rh1DG097400 Rh2AG448200 Rh2AG448300 Rh2AG511600 Rh2AG547500 Rh2AG551400 Rh2AG551700 Rh2AG555800 Rh2AG588000 Rh2BG565900 Rh2BG566000 Rh2BG566300 Rh2BG569000 Rh2BG600000 Rh2CG435200 Rh2CG435300 Rh2CG496600 Rh2CG535400 Rh2CG535600 Rh2CG539700 Rh2CG562700 Rh2CG570900 Rh2DG574600 Rh2DG574800 Rh2DG578600 Rh2DG610200 Rh4CG140900 Rh4DG127800 Rh5AG438500 Rh5BG436500 Rh5BG455500 Rh5CG477600 Rh5DG470300 Rh6AG129500 Rh6AG129900 Rh6BG125700 Rh6BG126000 Rh6BG126700 Rh6BG126800 Rh6CG124500 Rh6CG124800 Rh6CG125200 Rh6CG125300 Rh6DG111400 Rh6DG111700 Rh6DG111800 Rh7DG373300
rosa_wichuraiana Rw0G014010 Rw1G006140 Rw1G006160 Rw1G006180 Rw1G006190 Rw1G007340 Rw1G007360 Rw2G045660 Rw2G045670 Rw2G045970 Rw2G048220 Rw4G010820 Rw5G041010 Rw6G011220 Rw6G011230 Rw7G031490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 525, 608, 783, 789, 795, 801, 807, 813
AclI AACGTT 1 cut(s) 66
AcoI YGGCCR 1 cut(s) 582
AcsI RAATTY 2 cut(s) 258, 271
AdeI CACNNNGTG 1 cut(s) 453
AfaI GTAC 3 cut(s) 200, 325, 820
AfeI AGCGCT 1 cut(s) 118
AfiI CCNNNNNNNGG 5 cut(s) 91, 161, 162, 730, 782
AgsI TTSAA 1 cut(s) 266
AhlI ACTAGT 1 cut(s) 758
AjnI CCWGG 3 cut(s) 84, 193, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AluBI AGCT 5 cut(s) 176, 252, 566, 714, 870
AluI AGCT 5 cut(s) 176, 252, 566, 714, 870
AlwNI CAGNNNCTG 1 cut(s) 566
Aor51HI AGCGCT 1 cut(s) 118
AoxI GGCC 2 cut(s) 582, 732
ApeKI GCWGC 5 cut(s) 111, 114, 563, 566, 714
ApoI RAATTY 2 cut(s) 258, 271
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 205
AvaII GGWCC 1 cut(s) 205
BaeI ACNNNNGTAYC 2 cut(s) 810, 843
BalI TGGCCA 1 cut(s) 584
BanII GRGCYC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 802, 834
BbvI GCAGC 5 cut(s) 123, 126, 553, 575, 701
BccI CCATC 1 cut(s) 496
BciT130I CCWGG 3 cut(s) 86, 195, 777
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 7 cut(s) 177, 306, 444, 759, 842, 867, 871
BfmI CTRYAG 2 cut(s) 109, 529
BfoI RGCGCY 1 cut(s) 120
BisI GCNGC 6 cut(s) 112, 115, 526, 564, 567, 715
BlsI GCNGC 6 cut(s) 113, 116, 527, 565, 568, 716
Bme1390I CCNGG 3 cut(s) 86, 195, 777
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmrFI CCNGG 3 cut(s) 86, 195, 777
BmrI ACTGGG 1 cut(s) 328
BmsI GCATC 2 cut(s) 144, 595
BmuI ACTGGG 1 cut(s) 328
BoxI GACNNNNGTC 1 cut(s) 344
BpmI CTGGAG 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 570
BsaJI CCNNGG 2 cut(s) 194, 287
BsaWI WCCGGW 1 cut(s) 550
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 5 cut(s) 91, 161, 162, 730, 782
Bse1I ACTGG 2 cut(s) 323, 585
Bse3DI GCAATG 1 cut(s) 427
BseBI CCWGG 3 cut(s) 86, 195, 777
BseDI CCNNGG 2 cut(s) 194, 287
BseGI GGATG 2 cut(s) 94, 507
BseLI CCNNNNNNNGG 5 cut(s) 91, 161, 162, 730, 782
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 2 cut(s) 239, 584
BseNI ACTGG 2 cut(s) 323, 585
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 5 cut(s) 123, 126, 553, 575, 701
BshFI GGCC 2 cut(s) 584, 734
BsiSI CCGG 1 cut(s) 551
BslFI GGGAC 2 cut(s) 209, 812
BslI CCNNNNNNNGG 5 cut(s) 91, 161, 162, 730, 782
BsmFI GGGAC 2 cut(s) 209, 812
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 2 cut(s) 584, 734
Bsp1286I GDGCHC 1 cut(s) 479
Bsp143I GATC 4 cut(s) 42, 60, 649, 727
BspACI CCGC 8 cut(s) 525, 608, 783, 789, 795, 801, 807, 813
BspANI GGCC 2 cut(s) 584, 734
BspCNI CTCAG 2 cut(s) 240, 583
BspMAI CTGCAG 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 427
BsrI ACTGG 2 cut(s) 323, 585
BssECI CCNNGG 2 cut(s) 194, 287
BssMI GATC 4 cut(s) 42, 60, 649, 727
Bst2UI CCWGG 3 cut(s) 86, 195, 777
Bst4CI ACNGT 4 cut(s) 185, 295, 598, 684
BstAPI GCANNNNNTGC 1 cut(s) 453
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 2 cut(s) 248, 570
BstF5I GGATG 2 cut(s) 94, 507
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 4 cut(s) 45, 63, 652, 730
BstMBI GATC 4 cut(s) 42, 60, 649, 727
BstMWI GCNNNNNNNGC 5 cut(s) 453, 462, 572, 581, 720
BstNI CCWGG 3 cut(s) 86, 195, 777
BstPAI GACNNNNGTC 1 cut(s) 344
BstSCI CCNGG 3 cut(s) 84, 193, 775
BstSFI CTRYAG 2 cut(s) 109, 529
BstV1I GCAGC 5 cut(s) 123, 126, 553, 575, 701
BsuRI GGCC 2 cut(s) 584, 734
BtgZI GCGATG 1 cut(s) 618
BtsCI GGATG 2 cut(s) 94, 507
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 1 cut(s) 458
Cac8I GCNNGC 1 cut(s) 449
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 205
Csp6I GTAC 3 cut(s) 199, 324, 819
CspCI CAANNNNNGTGG 2 cut(s) 112, 147
CviAII CATG 1 cut(s) 400
CviQI GTAC 3 cut(s) 199, 324, 819
DdeI CTNAG 2 cut(s) 248, 570
DpnI GATC 4 cut(s) 44, 62, 651, 729
DpnII GATC 4 cut(s) 42, 60, 649, 727
DraIII CACNNNGTG 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 582
EciI GGCGGA 4 cut(s) 798, 804, 810, 816
Eco147I AGGCCT 1 cut(s) 734
Eco24I GRGCYC 1 cut(s) 479
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 205
Eco47III AGCGCT 1 cut(s) 118
EcoRI GAATTC 2 cut(s) 258, 271
EcoRII CCWGG 3 cut(s) 84, 193, 775
EcoRV GATATC 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 479
FaeI CATG 1 cut(s) 403
FaqI GGGAC 2 cut(s) 209, 812
FatI CATG 1 cut(s) 399
FauNDI CATATG 1 cut(s) 746
Fnu4HI GCNGC 6 cut(s) 112, 115, 526, 564, 567, 715
FokI GGATG 2 cut(s) 101, 514
FriOI GRGCYC 1 cut(s) 479
Fsp4HI GCNGC 6 cut(s) 112, 115, 526, 564, 567, 715
FspBI CTAG 7 cut(s) 177, 306, 444, 759, 842, 867, 871
GlaI GCGC 1 cut(s) 118
GluI GCNGC 6 cut(s) 112, 115, 526, 564, 567, 715
GsuI CTGGAG 1 cut(s) 798
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 2 cut(s) 584, 734
HapII CCGG 1 cut(s) 551
HhaI GCGC 1 cut(s) 119
Hin1II CATG 1 cut(s) 403
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HincII GTYRAC 2 cut(s) 349, 427
HindII GTYRAC 2 cut(s) 349, 427
HinfI GANTC 2 cut(s) 671, 708
HpaII CCGG 1 cut(s) 551
Hpy166II GTNNAC 3 cut(s) 169, 349, 427
Hpy188I TCNGA 3 cut(s) 249, 291, 339
Hpy188III TCNNGA 3 cut(s) 263, 675, 705
Hpy8I GTNNAC 3 cut(s) 169, 349, 427
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 4 cut(s) 185, 295, 598, 684
HpyCH4IV ACGT 4 cut(s) 66, 237, 404, 821
HpyCH4V TGCA 7 cut(s) 4, 111, 420, 456, 637, 657, 740
HpyF10VI GCNNNNNNNGC 5 cut(s) 453, 462, 572, 581, 720
HpyF3I CTNAG 2 cut(s) 248, 570
HpySE526I ACGT 4 cut(s) 66, 237, 404, 821
Hsp92II CATG 1 cut(s) 403
HspAI GCGC 1 cut(s) 117
Kzo9I GATC 4 cut(s) 42, 60, 649, 727
LmnI GCTCC 1 cut(s) 21
Lsp1109I GCAGC 5 cut(s) 123, 126, 553, 575, 701
LweI GCATC 2 cut(s) 144, 595
MaeI CTAG 7 cut(s) 177, 306, 444, 759, 842, 867, 871
MaeII ACGT 4 cut(s) 66, 237, 404, 821
MaeIII GTNAC 1 cut(s) 179
MalI GATC 4 cut(s) 44, 62, 651, 729
MboI GATC 4 cut(s) 42, 60, 649, 727
MboII GAAGA 1 cut(s) 510
MfeI CAATTG 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 479
MlsI TGGCCA 1 cut(s) 584
MluCI AATT 8 cut(s) 98, 124, 258, 271, 632, 700, 763, 847
MluNI TGGCCA 1 cut(s) 584
MmeI TCCRAC 2 cut(s) 362, 479
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 584
MseI TTAA 3 cut(s) 74, 297, 693
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 2 cut(s) 566, 714
MspI CCGG 1 cut(s) 551
MspR9I CCNGG 3 cut(s) 86, 195, 777
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 4
MvaI CCWGG 3 cut(s) 86, 195, 777
MwoI GCNNNNNNNGC 5 cut(s) 453, 462, 572, 581, 720
NdeI CATATG 1 cut(s) 746
NdeII GATC 4 cut(s) 42, 60, 649, 727
NlaIII CATG 1 cut(s) 403
NmeAIII GCCGAG 1 cut(s) 749
NsiI ATGCAT 1 cut(s) 6
PceI AGGCCT 1 cut(s) 734
PctI GAATGC 1 cut(s) 4
PfeI GAWTC 2 cut(s) 671, 708
PkrI GCNGC 6 cut(s) 113, 116, 527, 565, 568, 716
PshAI GACNNNNGTC 1 cut(s) 344
Psp1406I AACGTT 1 cut(s) 66
Psp6I CCWGG 3 cut(s) 84, 193, 775
PspGI CCWGG 3 cut(s) 84, 193, 775
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 1 cut(s) 113
PstNI CAGNNNCTG 1 cut(s) 566
PvuII CAGCTG 2 cut(s) 566, 714
RsaI GTAC 3 cut(s) 200, 325, 820
RsaNI GTAC 3 cut(s) 199, 324, 819
SaqAI TTAA 3 cut(s) 74, 297, 693
SatI GCNGC 6 cut(s) 112, 115, 526, 564, 567, 715
Sau3AI GATC 4 cut(s) 42, 60, 649, 727
Sau96I GGNCC 1 cut(s) 205
ScrFI CCNGG 3 cut(s) 86, 195, 777
SduI GDGCHC 1 cut(s) 479
SfaNI GCATC 2 cut(s) 144, 595
SfcI CTRYAG 2 cut(s) 109, 529
SinI GGWCC 1 cut(s) 205
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 8 cut(s) 98, 124, 258, 271, 632, 700, 763, 847
SseBI AGGCCT 1 cut(s) 734
SsiI CCGC 8 cut(s) 525, 608, 783, 789, 795, 801, 807, 813
SspMI CTAG 7 cut(s) 177, 306, 444, 759, 842, 867, 871
StuI AGGCCT 1 cut(s) 734
StyD4I CCNGG 3 cut(s) 84, 193, 775
TaaI ACNGT 4 cut(s) 185, 295, 598, 684
TaiI ACGT 4 cut(s) 69, 240, 407, 824
TaqI TCGA 2 cut(s) 59, 138
TasI AATT 8 cut(s) 98, 124, 258, 271, 632, 700, 763, 847
TauI GCSGC 1 cut(s) 528
TfiI GAWTC 2 cut(s) 671, 708
Tru1I TTAA 3 cut(s) 74, 297, 693
Tru9I TTAA 3 cut(s) 74, 297, 693
TscAI CASTG 1 cut(s) 458
TseI GCWGC 5 cut(s) 111, 114, 563, 566, 714
TspDTI ATGAA 2 cut(s) 284, 756
TspRI CASTG 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 2 cut(s) 258, 271
XspI CTAG 7 cut(s) 177, 306, 444, 759, 842, 867, 871
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.