Rroxscaffold_1G00058590

RNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000213
Physical Location & Seq
Reverse (-)
80494929 .. 80496083
1155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00058590.1

Sequence Viewer

Length: 1155 bp
ATGAAGTTGGAGGAGGAGGACGCTAACAAGTCCCTGTACATATGTTCCCGCGATTTCAACGTCGGACAACGGATCTCTTATGATATCCGCGCCATTAAGTTGTCCAATTTGTTATCGTCGTCTTCCTCTTTCGATCATCCGTTGCAATTACAGCAAGTGGCTTACAAAGCCGGCTCGGATCTCCCCGGTTCTGTGGGGTGTGGTGTTTGGGGCTCCAAAATTGTTTTCGCCGGCGGCTTGAAACCCAGCGTCCCTTACGGCATAGGGGCATCCAGATTAGGCCCAGTTGTTTGGCACAGGGATGTTTATTGCTTCGAAACGCAGCAGCCAGAGGACGAGGACGAGGACAATTTAATCAGGAAGATGGATGCTAGTTTTACTCTACTGCAAGCCAAATTTAGCCCTTACATTGTGGAGGTGTGCGGGAAGCTATATGCTCTCTCTTATAAGCTGGTCGCCGACCCTCCCGCCTTTGAGGTATTTGATCCCAAAGTGGGGAGTTGGGCGGCTCTGCCCCAACCTCCATTTTTCATACGTTTCTCTCCTTATCACCGTTCTGCTAGCTTCTCTTATGCACTTGCTGGCACCAAGATGTTTGTCTCCCATGACAAATGTCCCGTCTTCTGCTTCGATGTAGCACACCCACACAGAGAATGGAGACCAGTCCCTACTATGTGCCGAGGCGGCCCTTTCCCTTTTGTTGACAGATCTTTAGTCCTCGACCTGCCGGATGACAAGAAGAAGATAATGTTTGCCTATAGTCATGATCGTTATTGGCGGCTAGGAGTTTATCTTATGTCCTTGGTAGAAAACGCGGAATCCATCACACGAGTTGGTAATGTGAGGCTGCCACCGTTGCCTCTTGAGTTGGGGTACGCAAAACGCTGTCTATTCGTCCACATAGGAGGTCGGAAAGCCTGCCTTGTTGTAACCCAATTGGGTTTACCCAATGAAGTCCCACAACCGTATAAGCCTGGAACTCATAAGACATGGGGAGTTGCCATACCATTTCAATTTGAAGTTGATATGACCAAGGTGGACAATGGCAAGGAAGATTGTTTCACACTCCAATTCATGCCTCCTTGCGTCCTCAACTACCATACCAACCCATTCACTTTTCCAAACCCTGAAACAGCTGGTTGCTTTGTGCTCTGA

Protein Analysis

384

Amino Acids

43.17

Weight (kDa)

7.53

Isoelectric Point (pI)

44.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1668 PF07893 128 - 222 3.1e-09 Protein of unknown function (DUF1668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000213)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03120 FvH4_1g03530 FvH4_1g03650 FvH4_1g03720 FvH4_1g03730 FvH4_1g03800 FvH4_1g03820 FvH4_1g03870 FvH4_1g04520 FvH4_1g04650 FvH4_1g04850 FvH4_1g04870 FvH4_1g04892 FvH4_1g04980 FvH4_1g04981 FvH4_1g04982 FvH4_1g04991 FvH4_1g05020 FvH4_1g07301 FvH4_1g07310 FvH4_1g07350 FvH4_1g07350 FvH4_1g07350 FvH4_4g06201 FvH4_4g06202 FvH4_5g09162 FvH4_5g09163 FvH4_5g09240 FvH4_5g33421 FvH4_6g38540 FvH4_7g04610
malus_domestica MD02G1004600.v1.1
prunus_persica Prupe.6G029800_v2.0.a1 Prupe.7G110000_v2.0.a1
pyrus_communis pycom08g14630
rosa_chinensis RchiOBHm_Chr1g0329851 RchiOBHm_Chr2g0088891 RchiOBHm_Chr2g0089871 RchiOBHm_Chr2g0090141 RchiOBHm_Chr2g0090221 RchiOBHm_Chr2g0090631 RchiOBHm_Chr2g0090681 RchiOBHm_Chr4g0406891 RchiOBHm_Chr7g0225411
rosa_laevigata RLG00000001820 RLG00000009686 RLG00000015981 RLG00000016002 RLG00000016071 RLG00000016075 RLG00000016114 RLG00000016124 RLG00000016128 RLG00000016216 RLG00000019065 RLG00000029766 RLG00000029767 RLG00000029858 RLG00000032448
rosa_multiflora Rmu_sc0000226.1_g000014 Rmu_sc0000236.1_g000026 Rmu_sc0000236.1_g000028 Rmu_sc0000236.1_g000029 Rmu_sc0000236.1_g000036 Rmu_sc0000259.1_g000016 Rmu_sc0000259.1_g000017 Rmu_sc0002735.1_g000001 Rmu_sc0002759.1_g000075 Rmu_sc0002986.1_g000070 Rmu_sc0003459.1_g000002 Rmu_sc0003781.1_g000013 Rmu_sc0003952.1_g000005 Rmu_sc0003952.1_g000007 Rmu_sc0004526.1_g000002 Rmu_sc0005230.1_g000003 Rmu_sc0005424.1_g000013 Rmu_sc0005689.1_g000013 Rmu_sc0005689.1_g000014 Rmu_sc0005689.1_g000019 Rmu_sc0006945.1_g000041 Rmu_sc0008896.1_g000004 Rmu_sc0008968.1_g000008 Rmu_sc0009263.1_g000002 Rmu_sc0009263.1_g000003 Rmu_sc0016852.1_g000010 Rmu_sc0016852.1_g000012 Rmu_sc0019583.1_g000001 Rmu_sc0020367.1_g000002 Rmu_sc0021487.1_g000001 Rmu_sc0040185.1_g000002
rosa_roxburghii Rroxscaffold_1G00058590 Rroxscaffold_2G00150920 Rroxscaffold_2G00150950 Rroxscaffold_2G00150990 Rroxscaffold_2G00152360 Rroxscaffold_3G00227400 Rroxscaffold_3G00233830 Rroxscaffold_4G00301200 Rroxscaffold_4G00320610 Rroxscaffold_4G00332610 Rroxscaffold_5G00339640 Rroxscaffold_5G00351660
rosa_rugosa Rorug01G0091000 Rorug01G0091100 Rorug01G0486600 Rorug01G0488200 Rorug01G0488200 Rorug02G0009400 Rorug02G0009500 Rorug02G0009800 Rorug02G0012100 Rorug02G0012200 Rorug02G0016900 Rorug03G0363800.1
rosa_samantha Rh1BG080900 Rh1BG086600 Rh1DG118000 Rh1DG251300 Rh1DG251400 Rh2AG051500 Rh2AG054700 Rh2AG054800 Rh2AG055200 Rh2AG055400 Rh2AG055700 Rh2AG061900 Rh2AG062000 Rh2CG056200 Rh2DG362400 Rh4AG053200 Rh4BG051100 Rh4DG050000 Rh5CG153500 Rh7DG375300
rosa_wichuraiana Rw1G008030 Rw1G009110 Rw1G009170 Rw1G015600 Rw2G003250 Rw2G004020 Rw2G004550 Rw2G004690 Rw2G004710 Rw2G023940 Rw3G028880 Rw4G004290 Rw5G012660 Rw5G035010 Rw7G031660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 447
Acc36I ACCTGC 1 cut(s) 732
AccB1I GGYRCC 1 cut(s) 584
AccII CGCG 3 cut(s) 51, 90, 815
AciI CCGC 9 cut(s) 49, 88, 234, 423, 468, 506, 684, 778, 815
AclWI GGATC 3 cut(s) 80, 186, 479
AcsI RAATTY 1 cut(s) 395
AfaI GTAC 2 cut(s) 38, 875
AfiI CCNNNNNNNGG 2 cut(s) 494, 495
AgsI TTSAA 4 cut(s) 58, 241, 1013, 1019
AjnI CCWGG 1 cut(s) 973
AjuI GAANNNNNNNTTGG 2 cut(s) 209, 241
AluBI AGCT 4 cut(s) 430, 451, 564, 1136
AluI AGCT 4 cut(s) 430, 451, 564, 1136
Alw21I GWGCWC 1 cut(s) 1152
Alw26I GTCTC 2 cut(s) 604, 652
AlwI GGATC 3 cut(s) 80, 186, 479
AoxI GGCC 2 cut(s) 280, 685
ApeKI GCWGC 3 cut(s) 322, 325, 847
ApoI RAATTY 1 cut(s) 395
ArsI GACNNNNNNTTYG 2 cut(s) 603, 635
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 2 cut(s) 281, 686
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 315
AsuNHI GCTAGC 1 cut(s) 560
BanI GGYRCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 215
BauI CACGAG 1 cut(s) 828
BbsI GAAGAC 2 cut(s) 114, 613
Bbv12I GWGCWC 1 cut(s) 1152
BbvI GCAGC 3 cut(s) 334, 337, 834
BccI CCATC 2 cut(s) 358, 830
BceAI ACGGC 1 cut(s) 274
BciT130I CCWGG 1 cut(s) 975
BcnI CCSGG 1 cut(s) 186
BcoDI GTCTC 2 cut(s) 604, 652
BfaI CTAG 3 cut(s) 372, 561, 782
BfmI CTRYAG 1 cut(s) 757
BfuAI ACCTGC 1 cut(s) 732
BglI GCCNNNNNGGC 1 cut(s) 684
BglII AGATCT 1 cut(s) 707
BisI GCNGC 7 cut(s) 235, 323, 326, 507, 685, 779, 848
BlsI GCNGC 7 cut(s) 236, 324, 327, 508, 686, 780, 849
Bme1390I CCNGG 2 cut(s) 186, 975
BmgT120I GGNCC 2 cut(s) 281, 686
BmiI GGNNCC 2 cut(s) 214, 586
BmrFI CCNGG 2 cut(s) 186, 975
BmrI ACTGGG 1 cut(s) 278
BmsI GCATC 2 cut(s) 278, 358
BmtI GCTAGC 1 cut(s) 564
BmuI ACTGGG 1 cut(s) 278
BoxI GACNNNNGTC 1 cut(s) 612
BpiI GAAGAC 2 cut(s) 114, 613
Bpu14I TTCGAA 1 cut(s) 315
BpuEI CTTGAG 1 cut(s) 884
BpuMI CCSGG 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 652
BsaJI CCNNGG 4 cut(s) 184, 679, 801, 1032
Bsc4I CCNNNNNNNGG 2 cut(s) 494, 495
Bse118I RCCGGY 2 cut(s) 170, 230
Bse1I ACTGG 2 cut(s) 284, 662
BseBI CCWGG 1 cut(s) 975
BseDI CCNNGG 4 cut(s) 184, 679, 801, 1032
BseGI GGATG 5 cut(s) 136, 269, 307, 373, 736
BseLI CCNNNNNNNGG 2 cut(s) 494, 495
BseNI ACTGG 2 cut(s) 284, 662
BseRI GAGGAG 2 cut(s) 26, 29
BseXI GCAGC 3 cut(s) 334, 337, 834
BseYI CCCAGC 1 cut(s) 245
Bsh1236I CGCG 3 cut(s) 51, 90, 815
BshFI GGCC 2 cut(s) 282, 687
BshNI GGYRCC 1 cut(s) 584
BsiHKAI GWGCWC 1 cut(s) 1152
BsiSI CCGG 4 cut(s) 171, 186, 231, 728
BslFI GGGAC 5 cut(s) 16, 236, 600, 650, 941
BslI CCNNNNNNNGG 2 cut(s) 494, 495
BsmAI GTCTC 2 cut(s) 604, 652
BsmFI GGGAC 5 cut(s) 16, 236, 600, 650, 941
BsnI GGCC 2 cut(s) 282, 687
Bso31I GGTCTC 1 cut(s) 652
Bsp119I TTCGAA 1 cut(s) 315
Bsp1286I GDGCHC 2 cut(s) 215, 1152
Bsp1407I TGTACA 1 cut(s) 36
Bsp143I GATC 6 cut(s) 72, 133, 178, 484, 707, 766
BspACI CCGC 9 cut(s) 49, 88, 234, 423, 468, 506, 684, 778, 815
BspANI GGCC 2 cut(s) 282, 687
BspFNI CGCG 3 cut(s) 51, 90, 815
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 2 cut(s) 214, 586
BspMI ACCTGC 1 cut(s) 732
BspOI GCTAGC 1 cut(s) 564
BspPI GGATC 3 cut(s) 80, 186, 479
BspT104I TTCGAA 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 584
BspTNI GGTCTC 1 cut(s) 652
BsrFI RCCGGY 2 cut(s) 170, 230
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 2 cut(s) 284, 662
BssAI RCCGGY 2 cut(s) 170, 230
BssECI CCNNGG 4 cut(s) 184, 679, 801, 1032
BssMI GATC 6 cut(s) 72, 133, 178, 484, 707, 766
BssSI CACGAG 1 cut(s) 828
BssT1I CCWWGG 2 cut(s) 801, 1032
Bst2BI CACGAG 1 cut(s) 828
Bst2UI CCWGG 1 cut(s) 975
Bst4CI ACNGT 3 cut(s) 554, 855, 966
BstAUI TGTACA 1 cut(s) 36
BstBI TTCGAA 1 cut(s) 315
BstC8I GCNNGC 6 cut(s) 172, 232, 390, 562, 583, 919
BstF5I GGATG 5 cut(s) 136, 269, 307, 373, 736
BstFNI CGCG 3 cut(s) 51, 90, 815
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 6 cut(s) 75, 136, 181, 487, 710, 769
BstMAI GTCTC 2 cut(s) 604, 652
BstMBI GATC 6 cut(s) 72, 133, 178, 484, 707, 766
BstMWI GCNNNNNNNGC 4 cut(s) 151, 167, 684, 856
BstNI CCWGG 1 cut(s) 975
BstPAI GACNNNNGTC 1 cut(s) 612
BstSCI CCNGG 2 cut(s) 184, 973
BstSFI CTRYAG 1 cut(s) 757
BstUI CGCG 3 cut(s) 51, 90, 815
BstV1I GCAGC 3 cut(s) 334, 337, 834
BstV2I GAAGAC 2 cut(s) 114, 613
BstX2I RGATCY 3 cut(s) 72, 178, 707
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 3 cut(s) 72, 178, 707
BsuRI GGCC 2 cut(s) 282, 687
BtsCI GGATG 5 cut(s) 136, 269, 307, 373, 736
BveI ACCTGC 1 cut(s) 732
Cac8I GCNNGC 6 cut(s) 172, 232, 390, 562, 583, 919
CciI TCATGA 1 cut(s) 763
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 2 cut(s) 170, 230
Cfr13I GGNCC 2 cut(s) 281, 686
CseI GACGC 3 cut(s) 29, 238, 1075
Csp6I GTAC 2 cut(s) 37, 874
CviAII CATG 4 cut(s) 605, 764, 990, 1075
CviQI GTAC 2 cut(s) 37, 874
DpnI GATC 6 cut(s) 74, 135, 180, 486, 709, 768
DpnII GATC 6 cut(s) 72, 133, 178, 484, 707, 766
Eco130I CCWWGG 2 cut(s) 801, 1032
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 652
Eco32I GATATC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 973
EcoRV GATATC 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 801, 1032
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 2 cut(s) 801, 1032
FaeI CATG 4 cut(s) 608, 767, 993, 1078
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 5 cut(s) 16, 236, 600, 650, 941
FatI CATG 4 cut(s) 604, 763, 989, 1074
FauI CCCGC 3 cut(s) 56, 416, 475
FauNDI CATATG 1 cut(s) 41
Fnu4HI GCNGC 7 cut(s) 235, 323, 326, 507, 685, 779, 848
FokI GGATG 5 cut(s) 123, 256, 314, 380, 743
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 7 cut(s) 235, 323, 326, 507, 685, 779, 848
FspBI CTAG 3 cut(s) 372, 561, 782
GlaI GCGC 1 cut(s) 91
GluI GCNGC 7 cut(s) 235, 323, 326, 507, 685, 779, 848
GsaI CCCAGC 1 cut(s) 249
HaeIII GGCC 2 cut(s) 282, 687
HapII CCGG 4 cut(s) 171, 186, 231, 728
HgaI GACGC 3 cut(s) 29, 238, 1075
HhaI GCGC 1 cut(s) 92
Hin1II CATG 4 cut(s) 608, 767, 993, 1078
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HinfI GANTC 1 cut(s) 818
HpaII CCGG 4 cut(s) 171, 186, 231, 728
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 4 cut(s) 703, 898, 944, 1039
Hpy188I TCNGA 4 cut(s) 65, 178, 912, 1154
Hpy188III TCNNGA 4 cut(s) 273, 358, 764, 863
Hpy8I GTNNAC 4 cut(s) 703, 898, 944, 1039
Hpy99I CGWCG 2 cut(s) 65, 121
HpyCH4III ACNGT 3 cut(s) 554, 855, 966
HpyCH4IV ACGT 2 cut(s) 60, 535
HpyCH4V TGCA 3 cut(s) 145, 388, 575
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 167, 684, 856
HpySE526I ACGT 2 cut(s) 60, 535
Hsp92II CATG 4 cut(s) 608, 767, 993, 1078
HspAI GCGC 1 cut(s) 90
KroI GCCGGC 2 cut(s) 170, 230
KroNI GCCGGC 2 cut(s) 172, 232
Kzo9I GATC 6 cut(s) 72, 133, 178, 484, 707, 766
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 3 cut(s) 334, 337, 834
LweI GCATC 2 cut(s) 278, 358
MaeI CTAG 3 cut(s) 372, 561, 782
MaeII ACGT 2 cut(s) 60, 535
MaeIII GTNAC 1 cut(s) 928
MalI GATC 6 cut(s) 74, 135, 180, 486, 709, 768
MboI GATC 6 cut(s) 72, 133, 178, 484, 707, 766
MboII GAAGA 6 cut(s) 114, 373, 613, 751, 754, 1064
MfeI CAATTG 1 cut(s) 935
MflI RGATCY 3 cut(s) 72, 178, 707
MhlI GDGCHC 2 cut(s) 215, 1152
MluCI AATT 8 cut(s) 106, 146, 219, 349, 395, 935, 1013, 1070
MmeI TCCRAC 2 cut(s) 43, 890
MreI CGCCGGCG 1 cut(s) 230
MroNI GCCGGC 2 cut(s) 170, 230
MseI TTAA 2 cut(s) 96, 353
MslI CAYNNNNRTG 2 cut(s) 300, 590
MspA1I CMGCKG 1 cut(s) 1136
MspI CCGG 4 cut(s) 171, 186, 231, 728
MspR9I CCNGG 2 cut(s) 186, 975
MunI CAATTG 1 cut(s) 935
MvaI CCWGG 1 cut(s) 975
MvnI CGCG 3 cut(s) 51, 90, 815
MwoI GCNNNNNNNGC 4 cut(s) 151, 167, 684, 856
NaeI GCCGGC 2 cut(s) 172, 232
NciI CCSGG 1 cut(s) 186
NdeI CATATG 1 cut(s) 41
NdeII GATC 6 cut(s) 72, 133, 178, 484, 707, 766
NgoMIV GCCGGC 2 cut(s) 170, 230
NheI GCTAGC 1 cut(s) 560
NlaIII CATG 4 cut(s) 608, 767, 993, 1078
NlaIV GGNNCC 2 cut(s) 214, 586
NmeAIII GCCGAG 1 cut(s) 704
NspV TTCGAA 1 cut(s) 315
PagI TCATGA 1 cut(s) 763
PdiI GCCGGC 2 cut(s) 172, 232
PfeI GAWTC 1 cut(s) 818
PkrI GCNGC 7 cut(s) 236, 324, 327, 508, 686, 780, 849
PshAI GACNNNNGTC 1 cut(s) 612
PsiI TTATAA 1 cut(s) 447
Psp6I CCWGG 1 cut(s) 973
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 1 cut(s) 973
PspN4I GGNNCC 2 cut(s) 214, 586
PspPI GGNCC 2 cut(s) 281, 686
PsuI RGATCY 3 cut(s) 72, 178, 707
PvuII CAGCTG 1 cut(s) 1136
RsaI GTAC 2 cut(s) 38, 875
RsaNI GTAC 2 cut(s) 37, 874
RseI CAYNNNNRTG 2 cut(s) 300, 590
SaqAI TTAA 2 cut(s) 96, 353
SatI GCNGC 7 cut(s) 235, 323, 326, 507, 685, 779, 848
Sau3AI GATC 6 cut(s) 72, 133, 178, 484, 707, 766
Sau96I GGNCC 2 cut(s) 281, 686
ScrFI CCNGG 2 cut(s) 186, 975
SduI GDGCHC 2 cut(s) 215, 1152
SfaNI GCATC 2 cut(s) 278, 358
SfcI CTRYAG 1 cut(s) 757
SfuI TTCGAA 1 cut(s) 315
SgrAI CRCCGGYG 1 cut(s) 230
SmiMI CAYNNNNRTG 2 cut(s) 300, 590
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 8 cut(s) 106, 146, 219, 349, 395, 935, 1013, 1070
SsiI CCGC 9 cut(s) 49, 88, 234, 423, 468, 506, 684, 778, 815
SspMI CTAG 3 cut(s) 372, 561, 782
StyD4I CCNGG 2 cut(s) 184, 973
StyI CCWWGG 2 cut(s) 801, 1032
TaaI ACNGT 3 cut(s) 554, 855, 966
TaiI ACGT 2 cut(s) 63, 538
TaqI TCGA 4 cut(s) 132, 315, 630, 720
TasI AATT 8 cut(s) 106, 146, 219, 349, 395, 935, 1013, 1070
TatI WGTACW 1 cut(s) 36
TauI GCSGC 4 cut(s) 237, 509, 687, 781
TfiI GAWTC 1 cut(s) 818
Tru1I TTAA 2 cut(s) 96, 353
Tru9I TTAA 2 cut(s) 96, 353
TseI GCWGC 3 cut(s) 322, 325, 847
TspDTI ATGAA 4 cut(s) 17, 520, 966, 1063
TspGWI ACGGA 2 cut(s) 85, 129
XapI RAATTY 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 651
XspI CTAG 3 cut(s) 372, 561, 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.