FvH4_1g03331

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000107
Physical Location & Seq
Reverse (-)
1848607 .. 1851078
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g03331.t2

Sequence Viewer

Length: 1737 bp
ATGCAGCAAACTCTCGTTTCTTCCTTTTGCTTCATCTTGCTGGCTTTCTCCTCCATAGTCCTTGGTAAAAAACCCAACTGTGGGAAACATGGCCCGGTTATCAAATTCCCATTCAGCTTCAAAGGTAGCCACCCAGAAAATCCTGGGTTTCTTGTGTCCTGCAATGAAAAGAATGAAACCATTCTTGAGCTTCCAATCCCAGTTAAATTTGCAATCAAAACCGTAGACTATAAGGCTCAGAAAATCCAGCTATATGACCCAGAAGGTTGCTTGCTTGCCAAGCTTTTGAAAGTCCACAACATGTCAATCTCTCCCTTCCACTACTCAGAAAACCAAATGATTGATATTACCTTATTCAATTGTTCTTCAGCTAAAAGGAAAAAGCCGTCCTCGTGGCTTTATTCAGTCCCCTGCTTTGGCTACCAAATCTATTGGGTTTATTCTTTCGATAACATTGAGTACTTGCCCCTCCTGTCTTGTACAAAGATGCGTAATCTTTCATCAGTACCATACAGGACTGCACCTCGAGAGCTTTATTTGGAATGGTCTGAACCAAATTGTGGACTATGTGAAGCACAGGGTAGCACGAAGAAAGGAGGTTCGGGGAAAACTTTAGTAGCTACTGGTACATCCCTGGGTTCATTTGTACTCGTACTACTTGCTGGTGCAGCTTATCATGCTTATAGTTCTGATAGAAAGGAGAAACAGAATCAATTAAAAATTGAAAGCTTTTTAGAGGATTACAGAGCTCTCAAACCAAGCAGATACTCTTATTCAGACATCAAGAGGATTACAGATCAGTTCAAGGACAAATTAGGCCAAGGGGCATATGGGACTGTTTATAAGGGAAAGCTTTCTTCTGAATGTTTTGTTGCCGTGAAAGTCCTCACTAGTACTAAAGGAGATGGGGAAGAGTTTGTTAATGAAGTGGGAACAATGGGTCATATCCATCATGTCAATGTGGTTCGCTTGGTCGGATTCTGTGCTGATGGGTTTAGAAGAGCTCTTGTTTATGACTTCTTACCTAATGGTTCACTGCAAGATTTCATTTCATCAGCAGACAATAACAATTCTTTCCTTGGTTGGGATAAGTTGCAAGATATTGCTCTAGGCATAGCCAAAGGAATTGAATATCTGCACCAGGGGTGCGATCAACGAATCCTCCATTTTGATATCAAACCCCACAATGTTCTGCTTGACCATAACTTCACCCCAAAGATTTCTGATTTTGGTTTGGCCAAGTTATGTTCCAAGGATCAGAGTATAGTGTCAATGACTACAGCTAGGGGAACAATGGGGTACATTGCACCTGAAGTGTTCTCCAGGAACTTCGGGAATGTGTCCTATAAGTCAGATGTGTATAGTTATGGAATGGTACTGCTCGAGATGGTAGGAGGAAGAAAGAACATCAGTTCAACCACAGCGAACACACCTGAAGTTTACTACCCAGAATGGATCTATAATCTACTAGAAGAAGGTGAAGACCTCCGAATCCATGTTGGGGAAGAAGCAGATGCTCAAATTGCGAAGAGACTTGCGATTGTGGGTCTCTGGTGCATCCAATGGCACCCGGTTGATCGTCCCTCTATGAAAGGGGTGGTTCAGATGTTGGAAGGAGGAGAAAACTTGACTATGCCTCCAAATCCTTTTGCCTCTCAAGGTCCTGCAGGAACAAGTGCAAGCACACCTGTCAAAAGATTAAACCTTCAACTGGAACCAATTGCTGAGTTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

579

Amino Acids

64.43

Weight (kDa)

7.83

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 86 3.2e-10 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 259 - 528 1.6e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 260 - 526 2.1e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 1566
AccB7I CCANNNNNTGG 1 cut(s) 560
AccI GTMKAC 1 cut(s) 225
AclWI GGATC 2 cut(s) 1263, 1463
AcoI YGGCCR 1 cut(s) 1236
AcsI RAATTY 2 cut(s) 104, 206
AcuI CTGAAG 3 cut(s) 351, 1332, 1455
AfaI GTAC 9 cut(s) 461, 481, 507, 628, 648, 654, 895, 1301, 1377
AfiI CCNNNNNNNGG 7 cut(s) 80, 81, 416, 560, 1084, 1501, 1711
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 8 cut(s) 121, 289, 358, 725, 805, 1130, 1416, 1709
AhlI ACTAGT 1 cut(s) 890
AjnI CCWGG 4 cut(s) 142, 633, 1140, 1322
Alw21I GWGCWC 2 cut(s) 751, 1006
Alw26I GTCTC 2 cut(s) 1525, 1553
AlwI GGATC 2 cut(s) 1263, 1463
Ama87I CYCGRG 2 cut(s) 525, 1382
AoxI GGCC 3 cut(s) 91, 817, 1236
ApeKI GCWGC 2 cut(s) 4, 668
ApoI RAATTY 2 cut(s) 104, 206
Asp700I GAANNNNTTC 2 cut(s) 180, 853
AspS9I GGNCC 2 cut(s) 92, 1661
AsuC2I CCSGG 2 cut(s) 95, 1571
AsuHPI GGTGA 2 cut(s) 1201, 1490
AvaI CYCGRG 2 cut(s) 525, 1382
AvaII GGWCC 1 cut(s) 1661
BalI TGGCCA 1 cut(s) 1238
BanI GGYRCC 1 cut(s) 1566
BanII GRGCYC 2 cut(s) 751, 1006
BauI CACGAG 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 1488
Bbv12I GWGCWC 2 cut(s) 751, 1006
BbvI GCAGC 2 cut(s) 16, 680
BccI CCATC 4 cut(s) 899, 957, 983, 1381
BceAI ACGGC 2 cut(s) 370, 860
BciT130I CCWGG 4 cut(s) 144, 635, 1142, 1324
BcnI CCSGG 2 cut(s) 95, 1571
BcoDI GTCTC 2 cut(s) 1525, 1553
BcuI ACTAGT 1 cut(s) 890
BfaI CTAG 4 cut(s) 891, 1109, 1284, 1469
BfmI CTRYAG 2 cut(s) 1278, 1665
BisI GCNGC 2 cut(s) 5, 669
BlsI GCNGC 2 cut(s) 6, 670
BmcAI AGTACT 2 cut(s) 461, 895
Bme1390I CCNGG 6 cut(s) 95, 144, 635, 1142, 1324, 1571
Bme18I GGWCC 1 cut(s) 1661
BmeT110I CYCGRG 2 cut(s) 525, 1382
BmgT120I GGNCC 2 cut(s) 92, 1661
BmiI GGNNCC 2 cut(s) 1568, 1716
BmrFI CCNGG 6 cut(s) 95, 144, 635, 1142, 1324, 1571
BmrI ACTGGG 1 cut(s) 194
BmsI GCATC 3 cut(s) 477, 1504, 1566
BmuI ACTGGG 1 cut(s) 194
BpiI GAAGAC 1 cut(s) 1488
BpmI CTGGAG 1 cut(s) 1306
BpuEI CTTGAG 2 cut(s) 206, 1641
BpuMI CCSGG 2 cut(s) 95, 1571
BsaI GGTCTC 1 cut(s) 1553
BsaJI CCNNGG 8 cut(s) 61, 143, 633, 634, 820, 1078, 1141, 1251
BsaXI ACNNNNNCTCC 4 cut(s) 1146, 1176, 1621, 1651
Bsc4I CCNNNNNNNGG 7 cut(s) 80, 81, 416, 560, 1084, 1501, 1711
Bse1I ACTGG 3 cut(s) 200, 628, 1716
Bse3DI GCAATG 2 cut(s) 169, 1302
BseBI CCWGG 4 cut(s) 144, 635, 1142, 1324
BseDI CCNNGG 8 cut(s) 61, 143, 633, 634, 820, 1078, 1141, 1251
BseGI GGATG 2 cut(s) 629, 1557
BseLI CCNNNNNNNGG 7 cut(s) 80, 81, 416, 560, 1084, 1501, 1711
BseMI GCAATG 2 cut(s) 169, 1302
BseMII CTCAG 3 cut(s) 251, 339, 1716
BseNI ACTGG 3 cut(s) 200, 628, 1716
BseRI GAGGAG 2 cut(s) 40, 1632
BseXI GCAGC 2 cut(s) 16, 680
BsgI GTGCAG 3 cut(s) 504, 687, 1121
BshFI GGCC 3 cut(s) 93, 819, 1238
BshNI GGYRCC 1 cut(s) 1566
BsiHKAI GWGCWC 2 cut(s) 751, 1006
BsiHKCI CYCGRG 2 cut(s) 525, 1382
BsiSI CCGG 2 cut(s) 95, 1571
BslFI GGGAC 3 cut(s) 392, 847, 1566
BslI CCNNNNNNNGG 7 cut(s) 80, 81, 416, 560, 1084, 1501, 1711
BsmAI GTCTC 2 cut(s) 1525, 1553
BsmFI GGGAC 3 cut(s) 392, 847, 1566
BsnI GGCC 3 cut(s) 93, 819, 1238
Bso31I GGTCTC 1 cut(s) 1553
BsoBI CYCGRG 2 cut(s) 525, 1382
Bsp1286I GDGCHC 2 cut(s) 751, 1006
Bsp1407I TGTACA 1 cut(s) 479
Bsp143I GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
BspANI GGCC 3 cut(s) 93, 819, 1238
BspCNI CTCAG 3 cut(s) 250, 338, 1717
BspLI GGNNCC 2 cut(s) 1568, 1716
BspMAI CTGCAG 1 cut(s) 1669
BspPI GGATC 2 cut(s) 1263, 1463
BspQI GCTCTTC 1 cut(s) 994
BspT107I GGYRCC 1 cut(s) 1566
BspTNI GGTCTC 1 cut(s) 1553
BsrDI GCAATG 2 cut(s) 169, 1302
BsrGI TGTACA 1 cut(s) 479
BsrI ACTGG 3 cut(s) 200, 628, 1716
BssECI CCNNGG 8 cut(s) 61, 143, 633, 634, 820, 1078, 1141, 1251
BssMI GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
BssSI CACGAG 1 cut(s) 391
BssT1I CCWWGG 4 cut(s) 61, 820, 1078, 1251
Bst2BI CACGAG 1 cut(s) 391
Bst2UI CCWGG 4 cut(s) 144, 635, 1142, 1324
Bst4CI ACNGT 3 cut(s) 80, 223, 838
Bst6I CTCTTC 3 cut(s) 906, 994, 1523
BstAUI TGTACA 1 cut(s) 479
BstC8I GCNNGC 4 cut(s) 42, 272, 276, 1681
BstDEI CTNAG 3 cut(s) 237, 325, 1725
BstF5I GGATG 2 cut(s) 629, 1557
BstKTI GATC 5 cut(s) 799, 1153, 1258, 1458, 1579
BstMAI GTCTC 2 cut(s) 1525, 1553
BstMBI GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
BstMWI GCNNNNNNNGC 4 cut(s) 280, 668, 677, 1523
BstNI CCWGG 4 cut(s) 144, 635, 1142, 1324
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 6 cut(s) 93, 142, 633, 1140, 1322, 1569
BstSFI CTRYAG 2 cut(s) 1278, 1665
BstV1I GCAGC 2 cut(s) 16, 680
BstV2I GAAGAC 1 cut(s) 1488
BstX2I RGATCY 1 cut(s) 1455
BstYI RGATCY 1 cut(s) 1455
BsuRI GGCC 3 cut(s) 93, 819, 1238
BtsCI GGATG 2 cut(s) 629, 1557
BtsI GCAGTG 1 cut(s) 1034
BtsIMutI CAGTG 1 cut(s) 1034
Cac8I GCNNGC 4 cut(s) 42, 272, 276, 1681
Cfr13I GGNCC 2 cut(s) 92, 1661
Csp6I GTAC 9 cut(s) 460, 480, 506, 627, 647, 653, 894, 1300, 1376
CviAII CATG 5 cut(s) 89, 301, 677, 953, 1496
CviQI GTAC 9 cut(s) 460, 480, 506, 627, 647, 653, 894, 1300, 1376
DdeI CTNAG 3 cut(s) 237, 325, 1725
DpnI GATC 5 cut(s) 798, 1152, 1257, 1457, 1578
DpnII GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
EaeI YGGCCR 1 cut(s) 1236
Eam1104I CTCTTC 3 cut(s) 906, 994, 1523
EarI CTCTTC 3 cut(s) 906, 994, 1523
Ecl136II GAGCTC 2 cut(s) 749, 1004
Eco130I CCWWGG 4 cut(s) 61, 820, 1078, 1251
Eco24I GRGCYC 2 cut(s) 751, 1006
Eco31I GGTCTC 1 cut(s) 1553
Eco32I GATATC 1 cut(s) 1174
Eco47I GGWCC 1 cut(s) 1661
Eco53kI GAGCTC 2 cut(s) 749, 1004
Eco57I CTGAAG 3 cut(s) 351, 1332, 1455
Eco88I CYCGRG 2 cut(s) 525, 1382
EcoICRI GAGCTC 2 cut(s) 749, 1004
EcoO109I RGGNCCY 1 cut(s) 1661
EcoRII CCWGG 4 cut(s) 142, 633, 1140, 1322
EcoRV GATATC 1 cut(s) 1174
EcoT14I CCWWGG 4 cut(s) 61, 820, 1078, 1251
EcoT38I GRGCYC 2 cut(s) 751, 1006
ErhI CCWWGG 4 cut(s) 61, 820, 1078, 1251
FaeI CATG 5 cut(s) 92, 304, 680, 956, 1499
FaqI GGGAC 3 cut(s) 392, 847, 1566
FatI CATG 5 cut(s) 88, 300, 676, 952, 1495
FauNDI CATATG 1 cut(s) 829
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 5, 669
FokI GGATG 2 cut(s) 616, 1544
FriOI GRGCYC 2 cut(s) 751, 1006
Fsp4HI GCNGC 2 cut(s) 5, 669
FspBI CTAG 4 cut(s) 891, 1109, 1284, 1469
GluI GCNGC 2 cut(s) 5, 669
GsuI CTGGAG 1 cut(s) 1306
HaeIII GGCC 3 cut(s) 93, 819, 1238
HapII CCGG 2 cut(s) 95, 1571
Hin1II CATG 5 cut(s) 92, 304, 680, 956, 1499
HindIII AAGCTT 3 cut(s) 281, 727, 851
HinfI GANTC 4 cut(s) 709, 978, 1158, 1491
HpaII CCGG 2 cut(s) 95, 1571
HphI GGTGA 2 cut(s) 1201, 1490
Hpy166II GTNNAC 5 cut(s) 226, 295, 563, 1034, 1441
Hpy188III TCNNGA 5 cut(s) 185, 527, 784, 1333, 1384
Hpy8I GTNNAC 5 cut(s) 226, 295, 563, 1034, 1441
HpyAV CCTTC 5 cut(s) 257, 325, 1469, 1607, 1715
HpyCH4III ACNGT 3 cut(s) 80, 223, 838
HpyF10VI GCNNNNNNNGC 4 cut(s) 280, 668, 677, 1523
HpyF3I CTNAG 3 cut(s) 237, 325, 1725
Hsp92II CATG 5 cut(s) 92, 304, 680, 956, 1499
Kzo9I GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
LguI GCTCTTC 1 cut(s) 994
Lsp1109I GCAGC 2 cut(s) 16, 680
LweI GCATC 3 cut(s) 477, 1504, 1566
MaeI CTAG 4 cut(s) 891, 1109, 1284, 1469
MalI GATC 5 cut(s) 798, 1152, 1257, 1457, 1578
MboI GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
MfeI CAATTG 2 cut(s) 358, 1719
MflI RGATCY 1 cut(s) 1455
MhlI GDGCHC 2 cut(s) 751, 1006
MlsI TGGCCA 1 cut(s) 1238
MluNI TGGCCA 1 cut(s) 1238
MmeI TCCRAC 2 cut(s) 955, 1590
Mox20I TGGCCA 1 cut(s) 1238
MroXI GAANNNNTTC 2 cut(s) 180, 853
MscI TGGCCA 1 cut(s) 1238
MseI TTAA 4 cut(s) 204, 716, 921, 1700
MslI CAYNNNNRTG 1 cut(s) 957
Msp20I TGGCCA 1 cut(s) 1238
MspI CCGG 2 cut(s) 95, 1571
MspR9I CCNGG 6 cut(s) 95, 144, 635, 1142, 1324, 1571
MunI CAATTG 2 cut(s) 358, 1719
MvaI CCWGG 4 cut(s) 144, 635, 1142, 1324
MwoI GCNNNNNNNGC 4 cut(s) 280, 668, 677, 1523
NciI CCSGG 2 cut(s) 95, 1571
NdeI CATATG 1 cut(s) 829
NdeII GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
NlaIII CATG 5 cut(s) 92, 304, 680, 956, 1499
NlaIV GGNNCC 2 cut(s) 1568, 1716
NspI RCATGY 1 cut(s) 304
PaeR7I CTCGAG 2 cut(s) 525, 1382
PasI CCCWGGG 1 cut(s) 634
PciI ACATGT 1 cut(s) 300
PciSI GCTCTTC 1 cut(s) 994
PdmI GAANNNNTTC 2 cut(s) 180, 853
PfeI GAWTC 4 cut(s) 709, 978, 1158, 1491
PflMI CCANNNNNTGG 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 1322
PkrI GCNGC 2 cut(s) 6, 670
PpuMI RGGWCCY 1 cut(s) 1661
PscI ACATGT 1 cut(s) 300
PsiI TTATAA 1 cut(s) 843
Psp124BI GAGCTC 2 cut(s) 751, 1006
Psp5II RGGWCCY 1 cut(s) 1661
Psp6I CCWGG 4 cut(s) 142, 633, 1140, 1322
PspGI CCWGG 4 cut(s) 142, 633, 1140, 1322
PspN4I GGNNCC 2 cut(s) 1568, 1716
PspPI GGNCC 2 cut(s) 92, 1661
PspPPI RGGWCCY 1 cut(s) 1661
PstI CTGCAG 1 cut(s) 1669
PsuI RGATCY 1 cut(s) 1455
RsaI GTAC 9 cut(s) 461, 481, 507, 628, 648, 654, 895, 1301, 1377
RsaNI GTAC 9 cut(s) 460, 480, 506, 627, 647, 653, 894, 1300, 1376
RseI CAYNNNNRTG 1 cut(s) 957
SacI GAGCTC 2 cut(s) 751, 1006
SapI GCTCTTC 1 cut(s) 994
SaqAI TTAA 4 cut(s) 204, 716, 921, 1700
SatI GCNGC 2 cut(s) 5, 669
Sau3AI GATC 5 cut(s) 796, 1150, 1255, 1455, 1576
Sau96I GGNCC 2 cut(s) 92, 1661
SbfI CCTGCAGG 1 cut(s) 1669
ScaI AGTACT 2 cut(s) 461, 895
ScrFI CCNGG 6 cut(s) 95, 144, 635, 1142, 1324, 1571
SdaI CCTGCAGG 1 cut(s) 1669
SduI GDGCHC 2 cut(s) 751, 1006
SfaNI GCATC 3 cut(s) 477, 1504, 1566
SfcI CTRYAG 2 cut(s) 1278, 1665
Sfr274I CTCGAG 2 cut(s) 525, 1382
SinI GGWCC 1 cut(s) 1661
SlaI CTCGAG 2 cut(s) 525, 1382
SmiMI CAYNNNNRTG 1 cut(s) 957
SmlI CTYRAG 4 cut(s) 185, 525, 1382, 1656
SmoI CTYRAG 4 cut(s) 185, 525, 1382, 1656
SpeI ACTAGT 1 cut(s) 890
Sse8387I CCTGCAGG 1 cut(s) 1669
SspMI CTAG 4 cut(s) 891, 1109, 1284, 1469
SstI GAGCTC 2 cut(s) 751, 1006
StyD4I CCNGG 6 cut(s) 93, 142, 633, 1140, 1322, 1569
StyI CCWWGG 4 cut(s) 61, 820, 1078, 1251
TaaI ACNGT 3 cut(s) 80, 223, 838
TaqI TCGA 3 cut(s) 447, 526, 1383
TatI WGTACW 4 cut(s) 459, 479, 646, 893
TfiI GAWTC 4 cut(s) 709, 978, 1158, 1491
Tru1I TTAA 4 cut(s) 204, 716, 921, 1700
Tru9I TTAA 4 cut(s) 204, 716, 921, 1700
TscAI CASTG 1 cut(s) 1041
TseI GCWGC 2 cut(s) 4, 668
TspDTI ATGAA 9 cut(s) 22, 180, 189, 489, 630, 939, 1036, 1041, 1604
TspRI CASTG 1 cut(s) 1041
Van91I CCANNNNNTGG 1 cut(s) 560
VpaK11BI GGWCC 1 cut(s) 1661
XapI RAATTY 2 cut(s) 104, 206
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XhoI CTCGAG 2 cut(s) 525, 1382
XmiI GTMKAC 1 cut(s) 225
XmnI GAANNNNTTC 2 cut(s) 180, 853
XspI CTAG 4 cut(s) 891, 1109, 1284, 1469
ZrmI AGTACT 2 cut(s) 461, 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.