MD02G1253800.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000107
Physical Location & Seq
Forward (+)
30539606 .. 30541549
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1253800.v1.1.491

Sequence Viewer

Length: 1797 bp
ATGCAGAAATTGATCATCTCTTACTTTTGCATATTGTTACTGGTTTTCTTCAAACTAGTTGGAGCAAGCCATGCGTGCCTGGAATCCAAGTGTGGCGATGATCAAGGCGCAGCTATCCATTTCCCATTGCGCATCAGCCGCAAACATCATTGTGGAAATCCTGGGCTTGAATGCAATGTAAGATATGAGCAGCAAATACTAGTAAAATTATTTGTCAAACACATAGATTACAAGAGTCAGGAAATCCAAGTGTATCCCCCAAGTAGCTGCCTGCTGCTAAAGTCTGTTGAAATCCCAAACATGCCACCCTCTCCCTTCAACGTGTCAGTCGATGACATGAGTAACTTGACCTTATTCAGTTGTACTACTTCTGTTGCAAGCGATCCATATCCGTATCAATTCCCCTGCCTCGGCGACCCTGGCCGCATAATTTATGCCGTTGATTCCAGTTCTACATATCTATTGGAAGATCTCTTCCAGAAAGTACAATCTTGTTCAAAGATGTATGATGTTTTATCAGTTCCATTCGGTTCTTGGGTAGGCGGTTATAATGTTCGTTTCAAATGGTCTGAACCAAATTGCACAGTATGTGAAGCACAGGGAAAGAGGTGTAGGTGGAAGAACGATGGCACAAATAGTGAAATTAAATGTCTTGACTGGAAGAAACCAAGCAACACATGGAAAAAAGTGGTTACAGGTGGAATCCTGGGTGCTTTGCTTCTTGTTCTACTGATGATTGCAGCATATCGGGTACATAGCGCTGATAGAAAGGAGAAAGAGAGTCGATTAAGAATTGAAAGATTCTTGGAGGATTACAAAGCACTCAAACCAACCAGGTATTCGTACGCAGATATTAAGAGGATTACAAATCAATTCAAGGACAAGTTGGGCGAAGGAGCCTATGGAACTGTCTTCAAAGGAATGCTCTCTTCCGAATTCTTTATTGCTGTCAAAGTCCTCAACAGTTCCAAGGGAGATGGGGAAGAGTTCGTAAATGAAGTGCGAACGATGGGTCATATCCACCACGTCAACGTGGCTCGCTTGGTTGGATTTTGTGCTGATGGATTTGCACGAGCTCTTGTTTACGAGTTCTTCCCCAATGGTTCCCTGCAGGATTTCATTTCATCTGCAGATAGTAAGAATTCATTTCTTGGTTGGGATAAGTTGCAAGATATTGCCCTAGGCATAGCCAAAGGAATTGAATATCTTCACCAGGGATGCGATCTACGAATCCTGCATTTCGACATCAAACCCCATAATGTTTTGCTAGACCAAAACTTCACTCCAAAAATTTCTGATTTTGGTATGGCCAAGTTATGTTCCAAGGATCAAAGCTTGGTGTCCATGACTACAGCTAGGGGGACCATGGGCTACATTGCACCTGAAGTGTTTTCCAGGAATTTCGGAAATGTGTCTTACAAGGCAGATGTCTATAGCTTTGGAATGCTGCTGCTTGAGATGGTAGGAGGAAGGAAGAATATTGGTTCAATCACGGATAACACTACAAATGAAATTTACTATCCACAGTGGATTTATAATCTTCTAGAGGAAGGGGATGACCTACAAATCCACATTGGGGAAGAAGGAGATGGTAAAATTCCAAAGAAACTTGCAATTATAGGGCTATGGTGTATCCAGTGGCACCCGTCGGATCGTCCTTCCATGAAAATAGCGGTTCAGATGTTAGAAGGAGGAGGAGAAAGCTTGAGTATGCCGCCTAATCCCTTTGTGCCTACTGGTCCTACAACTAGAAATGCAAGACGCCTAGAGCTAGAAGCTATTGCTGAATTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

599

Amino Acids

67.16

Weight (kDa)

7.17

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 26 - 85 8e-07 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 291 - 560 1.4e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 292 - 560 1.3e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 549, 1536
Acc16I TGCGCA 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 1641
AciI CCGC 5 cut(s) 139, 424, 543, 1673, 1715
AclWI GGATC 3 cut(s) 377, 1335, 1659
AcoI YGGCCR 2 cut(s) 421, 1308
AcsI RAATTY 6 cut(s) 935, 1141, 1290, 1399, 1512, 1596
AcuI CTGAAG 1 cut(s) 1404
AcyI GRCGYC 1 cut(s) 1762
AfaI GTAC 4 cut(s) 364, 486, 753, 845
AfeI AGCGCT 1 cut(s) 760
AfiI CCNNNNNNNGG 2 cut(s) 410, 1576
AflIII ACRYGT 1 cut(s) 321
AhlI ACTAGT 2 cut(s) 55, 199
AjiI CACGTC 1 cut(s) 1027
AjnI CCWGG 7 cut(s) 78, 160, 418, 705, 833, 1212, 1394
AjuI GAANNNNNNNTTGG 2 cut(s) 823, 855
AluBI AGCT 9 cut(s) 113, 267, 1076, 1335, 1355, 1437, 1704, 1771, 1778
AluI AGCT 9 cut(s) 113, 267, 1076, 1335, 1355, 1437, 1704, 1771, 1778
Alw21I GWGCWC 1 cut(s) 1078
AlwI GGATC 3 cut(s) 377, 1335, 1659
Aor51HI AGCGCT 1 cut(s) 760
AoxI GGCC 2 cut(s) 421, 1308
ApeKI GCWGC 7 cut(s) 110, 190, 267, 274, 740, 1447, 1450
ApoI RAATTY 6 cut(s) 935, 1141, 1290, 1399, 1512, 1596
ArsI GACNNNNNNTTYG 2 cut(s) 997, 1029
Asp700I GAANNNNTTC 1 cut(s) 1206
AspA2I CCTAGG 1 cut(s) 1180
AspLEI GCGC 3 cut(s) 110, 132, 761
AspS9I GGNCC 2 cut(s) 1362, 1739
AsuHPI GGTGA 1 cut(s) 1202
AvaII GGWCC 2 cut(s) 1362, 1739
AvrII CCTAGG 1 cut(s) 1180
BalI TGGCCA 1 cut(s) 1310
BanI GGYRCC 1 cut(s) 1641
BanII GRGCYC 1 cut(s) 1078
BauI CACGAG 1 cut(s) 1071
BbsI GAAGAC 1 cut(s) 904
Bbv12I GWGCWC 1 cut(s) 1078
BbvI GCAGC 7 cut(s) 122, 202, 254, 261, 752, 1434, 1437
BccI CCATC 6 cut(s) 620, 971, 1003, 1055, 1453, 1583
BceAI ACGGC 1 cut(s) 422
BciT130I CCWGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
BciVI GTATCC 2 cut(s) 264, 1643
BclI TGATCA 2 cut(s) 12, 100
BcuI ACTAGT 2 cut(s) 55, 199
BfaI CTAG 9 cut(s) 56, 200, 1181, 1268, 1356, 1544, 1749, 1766, 1772
BfmI CTRYAG 4 cut(s) 1109, 1128, 1350, 1432
BfoI RGCGCY 1 cut(s) 762
BfuI GTATCC 2 cut(s) 264, 1643
BglII AGATCT 1 cut(s) 469
BlnI CCTAGG 1 cut(s) 1180
Bme1390I CCNGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
Bme18I GGWCC 2 cut(s) 1362, 1739
BmgBI CACGTC 1 cut(s) 1027
BmgT120I GGNCC 2 cut(s) 1362, 1739
BmiI GGNNCC 4 cut(s) 898, 1105, 1363, 1643
BmrFI CCNGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
BmsI GCATC 2 cut(s) 141, 1208
BpiI GAAGAC 1 cut(s) 904
BpuEI CTTGAG 2 cut(s) 1475, 1726
BsaBI GATNNNNATC 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 1762
BsaJI CCNNGG 9 cut(s) 161, 409, 418, 706, 969, 1180, 1213, 1323, 1365
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 1576
Bse1I ACTGG 5 cut(s) 45, 447, 662, 1636, 1741
Bse3DI GCAATG 3 cut(s) 125, 181, 1374
Bse8I GATNNNNATC 1 cut(s) 387
BseBI CCWGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
BseDI CCNNGG 9 cut(s) 161, 409, 418, 706, 969, 1180, 1213, 1323, 1365
BseGI GGATG 2 cut(s) 1223, 1561
BseJI GATNNNNATC 1 cut(s) 387
BseLI CCNNNNNNNGG 2 cut(s) 410, 1576
BseMI GCAATG 3 cut(s) 125, 181, 1374
BseNI ACTGG 5 cut(s) 45, 447, 662, 1636, 1741
BseRI GAGGAG 2 cut(s) 1707, 1710
BseXI GCAGC 7 cut(s) 122, 202, 254, 261, 752, 1434, 1437
BshFI GGCC 2 cut(s) 423, 1310
BshNI GGYRCC 1 cut(s) 1641
BsiHKAI GWGCWC 1 cut(s) 1078
BsiWI CGTACG 1 cut(s) 843
BslFI GGGAC 1 cut(s) 1375
BslI CCNNNNNNNGG 2 cut(s) 410, 1576
BsmFI GGGAC 1 cut(s) 1375
BsmI GAATGC 3 cut(s) 176, 927, 1449
BsnI GGCC 2 cut(s) 423, 1310
Bsp1286I GDGCHC 1 cut(s) 1078
Bsp143I GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
Bsp19I CCATGG 1 cut(s) 1365
BspACI CCGC 5 cut(s) 139, 424, 543, 1673, 1715
BspANI GGCC 2 cut(s) 423, 1310
BspLI GGNNCC 4 cut(s) 898, 1105, 1363, 1643
BspMAI CTGCAG 2 cut(s) 1113, 1132
BspPI GGATC 3 cut(s) 377, 1335, 1659
BspT107I GGYRCC 1 cut(s) 1641
BsrDI GCAATG 3 cut(s) 125, 181, 1374
BsrI ACTGG 5 cut(s) 45, 447, 662, 1636, 1741
BssECI CCNNGG 9 cut(s) 161, 409, 418, 706, 969, 1180, 1213, 1323, 1365
BssMI GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
BssNI GRCGYC 1 cut(s) 1762
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 4 cut(s) 969, 1180, 1323, 1365
Bst2BI CACGAG 1 cut(s) 1071
Bst2UI CCWGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
Bst4CI ACNGT 4 cut(s) 586, 910, 965, 1527
Bst6I CTCTTC 3 cut(s) 479, 934, 978
BstACI GRCGYC 1 cut(s) 1762
BstAPI GCANNNNNTGC 1 cut(s) 71
BstC8I GCNNGC 5 cut(s) 67, 76, 272, 379, 1039
BstDSI CCRYGG 1 cut(s) 1365
BstF5I GGATG 2 cut(s) 1223, 1561
BstH2I RGCGCY 1 cut(s) 762
BstHHI GCGC 3 cut(s) 110, 132, 761
BstKTI GATC 7 cut(s) 15, 103, 385, 472, 1225, 1330, 1654
BstMBI GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
BstMWI GCNNNNNNNGC 4 cut(s) 71, 75, 138, 420
BstNI CCWGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
BstNSI RCATGY 1 cut(s) 304
BstSCI CCNGG 7 cut(s) 78, 160, 418, 705, 833, 1212, 1394
BstSFI CTRYAG 4 cut(s) 1109, 1128, 1350, 1432
BstV1I GCAGC 7 cut(s) 122, 202, 254, 261, 752, 1434, 1437
BstV2I GAAGAC 1 cut(s) 904
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BsuI GTATCC 2 cut(s) 264, 1643
BsuRI GGCC 2 cut(s) 423, 1310
BtgI CCRYGG 1 cut(s) 1365
BtgZI GCGATG 1 cut(s) 111
BtrI CACGTC 1 cut(s) 1027
BtsCI GGATG 2 cut(s) 1223, 1561
BtsIMutI CAGTG 2 cut(s) 1532, 1643
Cac8I GCNNGC 5 cut(s) 67, 76, 272, 379, 1039
CfoI GCGC 3 cut(s) 110, 132, 761
Cfr13I GGNCC 2 cut(s) 1362, 1739
CseI GACGC 1 cut(s) 1770
CsiI ACCWGGT 1 cut(s) 833
Csp6I GTAC 4 cut(s) 363, 485, 752, 844
CviAII CATG 7 cut(s) 71, 301, 337, 678, 1345, 1366, 1663
CviQI GTAC 4 cut(s) 363, 485, 752, 844
DpnI GATC 7 cut(s) 14, 102, 384, 471, 1224, 1329, 1653
DpnII GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
EaeI YGGCCR 2 cut(s) 421, 1308
Eam1104I CTCTTC 3 cut(s) 479, 934, 978
EarI CTCTTC 3 cut(s) 479, 934, 978
Ecl136II GAGCTC 1 cut(s) 1076
Eco130I CCWWGG 4 cut(s) 969, 1180, 1323, 1365
Eco24I GRGCYC 1 cut(s) 1078
Eco47I GGWCC 2 cut(s) 1362, 1739
Eco47III AGCGCT 1 cut(s) 760
Eco53kI GAGCTC 1 cut(s) 1076
Eco57I CTGAAG 1 cut(s) 1404
EcoICRI GAGCTC 1 cut(s) 1076
EcoRI GAATTC 2 cut(s) 935, 1141
EcoRII CCWGG 7 cut(s) 78, 160, 418, 705, 833, 1212, 1394
EcoT14I CCWWGG 4 cut(s) 969, 1180, 1323, 1365
EcoT38I GRGCYC 1 cut(s) 1078
ErhI CCWWGG 4 cut(s) 969, 1180, 1323, 1365
FaeI CATG 7 cut(s) 74, 304, 340, 681, 1348, 1369, 1666
FaqI GGGAC 1 cut(s) 1375
FatI CATG 7 cut(s) 70, 300, 336, 677, 1344, 1365, 1662
FbaI TGATCA 2 cut(s) 12, 100
FokI GGATG 2 cut(s) 1230, 1568
FriOI GRGCYC 1 cut(s) 1078
FspBI CTAG 9 cut(s) 56, 200, 1181, 1268, 1356, 1544, 1749, 1766, 1772
FspI TGCGCA 1 cut(s) 131
GlaI GCGC 3 cut(s) 109, 131, 760
HaeII RGCGCY 1 cut(s) 762
HaeIII GGCC 2 cut(s) 423, 1310
HgaI GACGC 1 cut(s) 1770
HhaI GCGC 3 cut(s) 110, 132, 761
Hin1I GRCGYC 1 cut(s) 1762
Hin1II CATG 7 cut(s) 74, 304, 340, 681, 1348, 1369, 1666
Hin6I GCGC 3 cut(s) 108, 130, 759
HinP1I GCGC 3 cut(s) 108, 130, 759
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HindIII AAGCTT 2 cut(s) 1333, 1702
HinfI GANTC 7 cut(s) 83, 235, 443, 702, 781, 801, 1230
HphI GGTGA 1 cut(s) 1202
Hpy166II GTNNAC 2 cut(s) 1030, 1084
Hpy188I TCNGA 6 cut(s) 571, 934, 1297, 1406, 1651, 1680
Hpy188III TCNNGA 4 cut(s) 239, 478, 653, 1544
Hpy8I GTNNAC 2 cut(s) 1030, 1084
Hpy99I CGWCG 1 cut(s) 1651
HpyAV CCTTC 7 cut(s) 325, 887, 1464, 1544, 1577, 1668, 1682
HpyCH4III ACNGT 4 cut(s) 586, 910, 965, 1527
HpyCH4IV ACGT 3 cut(s) 321, 1026, 1032
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 75, 138, 420
HpySE526I ACGT 3 cut(s) 321, 1026, 1032
Hsp92I GRCGYC 1 cut(s) 1762
Hsp92II CATG 7 cut(s) 74, 304, 340, 681, 1348, 1369, 1666
HspAI GCGC 3 cut(s) 108, 130, 759
Ksp22I TGATCA 2 cut(s) 12, 100
Kzo9I GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
LmnI GCTCC 2 cut(s) 62, 896
Lsp1109I GCAGC 7 cut(s) 122, 202, 254, 261, 752, 1434, 1437
LweI GCATC 2 cut(s) 141, 1208
MabI ACCWGGT 1 cut(s) 833
MaeI CTAG 9 cut(s) 56, 200, 1181, 1268, 1356, 1544, 1749, 1766, 1772
MaeII ACGT 3 cut(s) 321, 1026, 1032
MaeIII GTNAC 3 cut(s) 36, 341, 691
MalI GATC 7 cut(s) 14, 102, 384, 471, 1224, 1329, 1653
MboI GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 1078
MlsI TGGCCA 1 cut(s) 1310
MluNI TGGCCA 1 cut(s) 1310
MlyI GAGTC 2 cut(s) 244, 790
MmeI TCCRAC 3 cut(s) 40, 1027, 1629
Mox20I TGGCCA 1 cut(s) 1310
MroXI GAANNNNTTC 1 cut(s) 1206
MscI TGGCCA 1 cut(s) 1310
MseI TTAA 3 cut(s) 645, 788, 855
MslI CAYNNNNRTG 1 cut(s) 150
Msp20I TGGCCA 1 cut(s) 1310
MspR9I CCNGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
Mva1269I GAATGC 3 cut(s) 176, 927, 1449
MvaI CCWGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
MwoI GCNNNNNNNGC 4 cut(s) 71, 75, 138, 420
NcoI CCATGG 1 cut(s) 1365
NdeII GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
NlaIII CATG 7 cut(s) 74, 304, 340, 681, 1348, 1369, 1666
NlaIV GGNNCC 4 cut(s) 898, 1105, 1363, 1643
NmeAIII GCCGAG 1 cut(s) 390
NsbI TGCGCA 1 cut(s) 131
NspI RCATGY 1 cut(s) 304
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PctI GAATGC 3 cut(s) 176, 927, 1449
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 5 cut(s) 83, 443, 702, 801, 1230
Pfl23II CGTACG 1 cut(s) 843
PfoI TCCNGGA 1 cut(s) 1394
PleI GAGTC 2 cut(s) 243, 789
PpsI GAGTC 2 cut(s) 243, 789
PsiI TTATAA 2 cut(s) 549, 1536
Psp124BI GAGCTC 1 cut(s) 1078
Psp6I CCWGG 7 cut(s) 78, 160, 418, 705, 833, 1212, 1394
PspGI CCWGG 7 cut(s) 78, 160, 418, 705, 833, 1212, 1394
PspLI CGTACG 1 cut(s) 843
PspN4I GGNNCC 4 cut(s) 898, 1105, 1363, 1643
PspPI GGNCC 2 cut(s) 1362, 1739
PstI CTGCAG 2 cut(s) 1113, 1132
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 4 cut(s) 364, 486, 753, 845
RsaNI GTAC 4 cut(s) 363, 485, 752, 844
RseI CAYNNNNRTG 1 cut(s) 150
SacI GAGCTC 1 cut(s) 1078
SaqAI TTAA 3 cut(s) 645, 788, 855
Sau3AI GATC 7 cut(s) 12, 100, 382, 469, 1222, 1327, 1651
Sau96I GGNCC 2 cut(s) 1362, 1739
SbfI CCTGCAGG 1 cut(s) 1113
SchI GAGTC 2 cut(s) 244, 790
ScrFI CCNGG 7 cut(s) 80, 162, 420, 707, 835, 1214, 1396
SdaI CCTGCAGG 1 cut(s) 1113
SduI GDGCHC 1 cut(s) 1078
SexAI ACCWGGT 1 cut(s) 833
SfaNI GCATC 2 cut(s) 141, 1208
SfcI CTRYAG 4 cut(s) 1109, 1128, 1350, 1432
SinI GGWCC 2 cut(s) 1362, 1739
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 2 cut(s) 1454, 1705
SmoI CTYRAG 2 cut(s) 1454, 1705
SpeI ACTAGT 2 cut(s) 55, 199
Sse8387I CCTGCAGG 1 cut(s) 1113
SsiI CCGC 5 cut(s) 139, 424, 543, 1673, 1715
SspI AATATT 1 cut(s) 1480
SspMI CTAG 9 cut(s) 56, 200, 1181, 1268, 1356, 1544, 1749, 1766, 1772
SstI GAGCTC 1 cut(s) 1078
StyD4I CCNGG 7 cut(s) 78, 160, 418, 705, 833, 1212, 1394
StyI CCWWGG 4 cut(s) 969, 1180, 1323, 1365
TaaI ACNGT 4 cut(s) 586, 910, 965, 1527
TaiI ACGT 3 cut(s) 324, 1029, 1035
TaqI TCGA 3 cut(s) 330, 784, 1242
TatI WGTACW 2 cut(s) 362, 484
TauI GCSGC 3 cut(s) 141, 426, 1717
TfiI GAWTC 5 cut(s) 83, 443, 702, 801, 1230
Tru1I TTAA 3 cut(s) 645, 788, 855
Tru9I TTAA 3 cut(s) 645, 788, 855
TscAI CASTG 2 cut(s) 1532, 1643
TseI GCWGC 7 cut(s) 110, 190, 267, 274, 740, 1447, 1450
TspDTI ATGAA 6 cut(s) 1011, 1108, 1113, 1134, 1524, 1679
TspGWI ACGGA 2 cut(s) 381, 1508
TspRI CASTG 2 cut(s) 1532, 1643
VpaK11BI GGWCC 2 cut(s) 1362, 1739
XapI RAATTY 6 cut(s) 935, 1141, 1290, 1399, 1512, 1596
XbaI TCTAGA 1 cut(s) 1543
XceI RCATGY 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 2 cut(s) 531, 675
XmaJI CCTAGG 1 cut(s) 1180
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 9 cut(s) 56, 200, 1181, 1268, 1356, 1544, 1749, 1766, 1772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.