Rw0G022840

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01059
OG0000107
Physical Location & Seq
Reverse (-)
26954 .. 28961
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022840.1

Sequence Viewer

Length: 1587 bp
ATGACTTGCTTCGAAGTGATAGAAGCGTGTTCCAAATCCAAGTGTGGGGATAGTGGTCTGGCTATCCATCTCCCTCTCCACCGCGGACACCATTGTGGACCGCAATACAGTGAGTGCAATGAAGCCACAGACGGTGATGACCAGCAAATCTTACTAGTGAAATTCTTTGTCACACACATAGATTATATCAATCAGGAAATCCAAGTACATAACTCAGATAATTGCCTACTGCTAAATCCTTTAGACATCCCATACGCCAACATGCCAAAGTCTCCCTTCGAGACCTTGGAATCTGAAAGGGGTAACATTACCTTATTCCGTTGTCCTGCTAAAACAGTTGTAACTTATTATCATGATTATGCACCACTCGTCCCCTGCCTAGGTGACACTGACTACAATATTTATGCTCTTTTTTCTGACACTGAAATTGGGGATTTGCTCGGCATAGAGTCTTGCACAAAGATGTATGATGTTTTATCACTTCCATATAATACTGCATATGATTCGACTCAAACTCTTCGTTTCAAATGGTCTACACCAAATTGTTCAGAATGTGAAGCATTGGGCAACATGTGTAGATTGAAGAACAGCCGCACCCAAAGTAAAATTGAATGTGTTCAGTTGAGGAAACCAAATTTGAAATCGAAATTAGTGGCAGCAGGTTCAACTCTGGGTTCATTTTTGTCCTACTACTTGCCATTGCACATTGCAATCCATCGTTTCTATACATGGAATAGAAATGAAAATGAACATCAATTCAAGGTTATAAGATTTTTAGAGGATTACAAAGCTCTCAAGCCAAGCAGATATTCTTATGCAGATATTAAGAGGATTACAAATGAATTCAAGGAAAAGCTGGGTGAAGGAGCCTATGGAACTGTTTTTAAAGGAAGTCTATCTTCTGAATTCTTTGTTGCTGTCAAAGTCCTAAATAATTCTAAGGGAAATGGGGAAGAGTTTGTCAATGAAGTGGGAGCAATGGGTCATATCCACCATGTCAACGTGGTTCGAATGGTTGGCTTTTGTGCTGATGGATACAGAAGAGCTCTTGTTTATGAGTACTTCCCCAATGGTTCATTACAAGATTTCATTTCCTCAGCAGACAATAAGAATGGTTTCCTTGGTTGGGATAAGTTGAAAGATATTTCTCTAGGAATAGCCAAAGGAATTGAATATCTCCACCAAGGATGTGATCAACGAATCCTCCATTTTGATATCAAACCTCATAATGTTTTGCTAGACCACAACTTCACTCCAAAAATTTCTGATTTTGGTTTGGCCAAGTTATGTTCCAAGGATCAAAGTATTGTGTCAATGACAACGGCTAGGGGCACCATGGGCTACATTGCACCTGAAGTTTTCTCCAGAAATTTTGGAAATGTGTCTTACAAGTCTGATGTCTATAGCTTCGGAATGTTGTTGCTGGAGATCGTTGGAGGTAGGAAGAATATTGGTCCCACCATTAATGATGCTAGTGACATTACTATCAATCCTTTTGGCTCTACAGGTCCGACTAGAACAAATGCAAGTCTCCCAGCTTTACGACGCTTGAACCTTGAGTTAGAAGCAATTGCTGAATTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

528

Amino Acids

59.27

Weight (kDa)

6.2

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 281 - 479 2.4e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 282 - 482 8.8e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 1331
AccI GTMKAC 1 cut(s) 533
AccII CGCG 1 cut(s) 84
AciI CCGC 4 cut(s) 82, 84, 101, 592
AclWI GGATC 1 cut(s) 1305
AcoI YGGCCR 1 cut(s) 1278
AcsI RAATTY 6 cut(s) 161, 634, 842, 905, 1260, 1369
AcuI CTGAAG 1 cut(s) 1374
AfaI GTAC 2 cut(s) 207, 1061
AfiI CCNNNNNNNGG 3 cut(s) 45, 380, 1126
AflIII ACRYGT 1 cut(s) 570
AhlI ACTAGT 1 cut(s) 154
AjuI GAANNNNNNNTTGG 2 cut(s) 793, 825
AleI CACNNNNGTG 1 cut(s) 93
AluBI AGCT 5 cut(s) 791, 856, 1046, 1407, 1538
AluI AGCT 5 cut(s) 791, 856, 1046, 1407, 1538
Alw21I GWGCWC 1 cut(s) 1048
Alw26I GTCTC 3 cut(s) 275, 276, 1535
AlwI GGATC 1 cut(s) 1305
AoxI GGCC 1 cut(s) 1278
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 6 cut(s) 161, 634, 842, 905, 1260, 1369
AseI ATTAAT 1 cut(s) 1464
Asp700I GAANNNNTTC 2 cut(s) 615, 1115
AspA2I CCTAGG 1 cut(s) 379
AspS9I GGNCC 3 cut(s) 98, 1454, 1508
AsuHPI GGTGA 3 cut(s) 146, 395, 872
AsuII TTCGAA 2 cut(s) 12, 1009
AvaII GGWCC 3 cut(s) 98, 1454, 1508
AvrII CCTAGG 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 1334
BalI TGGCCA 1 cut(s) 1280
BanI GGYRCC 1 cut(s) 1331
BanII GRGCYC 1 cut(s) 1048
Bbv12I GWGCWC 1 cut(s) 1048
BbvCI CCTCAGC 1 cut(s) 1096
BbvI GCAGC 1 cut(s) 668
BccI CCATC 3 cut(s) 75, 723, 1025
BceAI ACGGC 1 cut(s) 1338
BcgI CGANNNNNNTGC 2 cut(s) 486, 520
BciVI GTATCC 1 cut(s) 1028
BclI TGATCA 1 cut(s) 1192
BcoDI GTCTC 3 cut(s) 275, 276, 1535
BcuI ACTAGT 1 cut(s) 154
BfaI CTAG 7 cut(s) 155, 380, 1151, 1238, 1326, 1473, 1515
BfmI CTRYAG 2 cut(s) 1402, 1503
BfuAI ACCTGC 1 cut(s) 650
BfuI GTATCC 1 cut(s) 1028
BisI GCNGC 2 cut(s) 592, 657
BlnI CCTAGG 1 cut(s) 379
BlsI GCNGC 2 cut(s) 593, 658
BmcAI AGTACT 1 cut(s) 1061
Bme18I GGWCC 3 cut(s) 98, 1454, 1508
BmgT120I GGNCC 3 cut(s) 98, 1454, 1508
BmiI GGNNCC 3 cut(s) 868, 1333, 1456
BmsI GCATC 1 cut(s) 1459
BpmI CTGGAG 2 cut(s) 1348, 1445
Bpu10I CCTNAGC 1 cut(s) 1096
Bpu14I TTCGAA 2 cut(s) 12, 1009
BpuEI CTTGAG 2 cut(s) 779, 1577
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 7 cut(s) 82, 285, 379, 1120, 1183, 1293, 1335
BsaXI ACNNNNNCTCC 2 cut(s) 1188, 1218
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 380, 1126
Bse3DI GCAATG 5 cut(s) 124, 698, 705, 984, 1344
BseDI CCNNGG 7 cut(s) 82, 285, 379, 1120, 1183, 1293, 1335
BseGI GGATG 2 cut(s) 246, 1193
BseLI CCNNNNNNNGG 3 cut(s) 45, 380, 1126
BseMI GCAATG 5 cut(s) 124, 698, 705, 984, 1344
BseMII CTCAG 2 cut(s) 228, 1110
BseSI GKGCMC 1 cut(s) 1334
BseXI GCAGC 1 cut(s) 668
BseYI CCCAGC 2 cut(s) 856, 1534
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 1 cut(s) 1280
BshNI GGYRCC 1 cut(s) 1331
BsiHKAI GWGCWC 1 cut(s) 1048
BslFI GGGAC 2 cut(s) 356, 1440
BslI CCNNNNNNNGG 3 cut(s) 45, 380, 1126
BsmAI GTCTC 3 cut(s) 275, 276, 1535
BsmFI GGGAC 2 cut(s) 356, 1440
BsnI GGCC 1 cut(s) 1280
Bso31I GGTCTC 1 cut(s) 275
Bsp119I TTCGAA 2 cut(s) 12, 1009
Bsp1286I GDGCHC 2 cut(s) 1048, 1334
Bsp143I GATC 3 cut(s) 1192, 1297, 1428
Bsp19I CCATGG 1 cut(s) 1335
BspACI CCGC 4 cut(s) 82, 84, 101, 592
BspANI GGCC 1 cut(s) 1280
BspCNI CTCAG 2 cut(s) 227, 1109
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 3 cut(s) 868, 1333, 1456
BspMI ACCTGC 1 cut(s) 650
BspPI GGATC 1 cut(s) 1305
BspQI GCTCTTC 1 cut(s) 1036
BspT104I TTCGAA 2 cut(s) 12, 1009
BspT107I GGYRCC 1 cut(s) 1331
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 5 cut(s) 124, 698, 705, 984, 1344
BssECI CCNNGG 7 cut(s) 82, 285, 379, 1120, 1183, 1293, 1335
BssMI GATC 3 cut(s) 1192, 1297, 1428
BssT1I CCWWGG 6 cut(s) 285, 379, 1120, 1183, 1293, 1335
Bst4CI ACNGT 4 cut(s) 110, 134, 337, 880
Bst6I CTCTTC 3 cut(s) 522, 948, 1036
BstBI TTCGAA 2 cut(s) 12, 1009
BstDEI CTNAG 3 cut(s) 214, 939, 1096
BstDSI CCRYGG 2 cut(s) 82, 1335
BstF5I GGATG 2 cut(s) 246, 1193
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 3 cut(s) 1195, 1300, 1431
BstMAI GTCTC 3 cut(s) 275, 276, 1535
BstMBI GATC 3 cut(s) 1192, 1297, 1428
BstMWI GCNNNNNNNGC 1 cut(s) 1338
BstNSI RCATGY 2 cut(s) 265, 574
BstSFI CTRYAG 2 cut(s) 1402, 1503
BstSLI GKGCMC 1 cut(s) 1334
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 1 cut(s) 668
BsuI GTATCC 1 cut(s) 1028
BsuRI GGCC 1 cut(s) 1280
BtgI CCRYGG 2 cut(s) 82, 1335
BtsCI GGATG 2 cut(s) 246, 1193
BtsIMutI CAGTG 3 cut(s) 115, 387, 420
BveI ACCTGC 1 cut(s) 650
CciI TCATGA 1 cut(s) 352
Cfr13I GGNCC 3 cut(s) 98, 1454, 1508
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 1 cut(s) 1554
Csp6I GTAC 2 cut(s) 206, 1060
CviAII CATG 6 cut(s) 262, 353, 571, 729, 995, 1336
CviQI GTAC 2 cut(s) 206, 1060
DdeI CTNAG 3 cut(s) 214, 939, 1096
DpnI GATC 3 cut(s) 1194, 1299, 1430
DpnII GATC 3 cut(s) 1192, 1297, 1428
DraI TTTAAA 1 cut(s) 886
EaeI YGGCCR 1 cut(s) 1278
Eam1104I CTCTTC 3 cut(s) 522, 948, 1036
EarI CTCTTC 3 cut(s) 522, 948, 1036
Ecl136II GAGCTC 1 cut(s) 1046
Eco130I CCWWGG 6 cut(s) 285, 379, 1120, 1183, 1293, 1335
Eco24I GRGCYC 1 cut(s) 1048
Eco31I GGTCTC 1 cut(s) 275
Eco32I GATATC 1 cut(s) 1216
Eco47I GGWCC 3 cut(s) 98, 1454, 1508
Eco53kI GAGCTC 1 cut(s) 1046
Eco57I CTGAAG 1 cut(s) 1374
EcoICRI GAGCTC 1 cut(s) 1046
EcoRI GAATTC 2 cut(s) 842, 905
EcoRV GATATC 1 cut(s) 1216
EcoT14I CCWWGG 6 cut(s) 285, 379, 1120, 1183, 1293, 1335
EcoT38I GRGCYC 1 cut(s) 1048
ErhI CCWWGG 6 cut(s) 285, 379, 1120, 1183, 1293, 1335
FaeI CATG 6 cut(s) 265, 356, 574, 732, 998, 1339
FalI AAGNNNNNCTT 4 cut(s) 260, 292, 883, 915
FaqI GGGAC 2 cut(s) 356, 1440
FatI CATG 6 cut(s) 261, 352, 570, 728, 994, 1335
FauNDI CATATG 1 cut(s) 499
FbaI TGATCA 1 cut(s) 1192
FblI GTMKAC 1 cut(s) 533
Fnu4HI GCNGC 2 cut(s) 592, 657
FokI GGATG 2 cut(s) 233, 1200
FriOI GRGCYC 1 cut(s) 1048
Fsp4HI GCNGC 2 cut(s) 592, 657
FspBI CTAG 7 cut(s) 155, 380, 1151, 1238, 1326, 1473, 1515
GluI GCNGC 2 cut(s) 592, 657
GsaI CCCAGC 2 cut(s) 860, 1538
GsuI CTGGAG 2 cut(s) 1348, 1445
HaeIII GGCC 1 cut(s) 1280
HgaI GACGC 1 cut(s) 1554
Hin1II CATG 6 cut(s) 265, 356, 574, 732, 998, 1339
HincII GTYRAC 1 cut(s) 1000
HindII GTYRAC 1 cut(s) 1000
HinfI GANTC 5 cut(s) 290, 449, 503, 508, 1200
HphI GGTGA 3 cut(s) 146, 395, 872
Hpy166II GTNNAC 3 cut(s) 98, 534, 1000
Hpy188I TCNGA 9 cut(s) 217, 295, 418, 550, 904, 1267, 1396, 1412, 1512
Hpy188III TCNNGA 4 cut(s) 194, 280, 353, 1365
Hpy8I GTNNAC 3 cut(s) 98, 534, 1000
Hpy99I CGWCG 1 cut(s) 1548
HpyAV CCTTC 2 cut(s) 286, 857
HpyCH4III ACNGT 4 cut(s) 110, 134, 337, 880
HpyCH4IV ACGT 1 cut(s) 1002
HpyCH4V TGCA 9 cut(s) 117, 362, 456, 497, 703, 710, 818, 1349, 1526
HpyF10VI GCNNNNNNNGC 1 cut(s) 1338
HpyF3I CTNAG 3 cut(s) 214, 939, 1096
HpySE526I ACGT 1 cut(s) 1002
Hsp92II CATG 6 cut(s) 265, 356, 574, 732, 998, 1339
Ksp22I TGATCA 1 cut(s) 1192
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 3 cut(s) 1192, 1297, 1428
LguI GCTCTTC 1 cut(s) 1036
LmnI GCTCC 2 cut(s) 866, 974
Lsp1109I GCAGC 1 cut(s) 668
LweI GCATC 1 cut(s) 1459
MaeI CTAG 7 cut(s) 155, 380, 1151, 1238, 1326, 1473, 1515
MaeII ACGT 1 cut(s) 1002
MaeIII GTNAC 5 cut(s) 169, 302, 340, 383, 1475
MalI GATC 3 cut(s) 1194, 1299, 1430
MboI GATC 3 cut(s) 1192, 1297, 1428
MboII GAAGA 6 cut(s) 509, 595, 891, 965, 1053, 1456
MfeI CAATTG 1 cut(s) 1569
MhlI GDGCHC 2 cut(s) 1048, 1334
MlsI TGGCCA 1 cut(s) 1280
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 2 cut(s) 458, 502
MmeI TCCRAC 2 cut(s) 1414, 1535
MnlI CCTC 8 cut(s) 84, 618, 772, 822, 1105, 1214, 1233, 1430
Mox20I TGGCCA 1 cut(s) 1280
MroXI GAANNNNTTC 2 cut(s) 615, 1115
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 3 cut(s) 825, 885, 1464
MslI CAYNNNNRTG 3 cut(s) 93, 357, 461
Msp20I TGGCCA 1 cut(s) 1280
MspA1I CMGCKG 1 cut(s) 84
MunI CAATTG 1 cut(s) 1569
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 1338
NcoI CCATGG 1 cut(s) 1335
NdeI CATATG 1 cut(s) 499
NdeII GATC 3 cut(s) 1192, 1297, 1428
NlaIII CATG 6 cut(s) 265, 356, 574, 732, 998, 1339
NlaIV GGNNCC 3 cut(s) 868, 1333, 1456
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 3 cut(s) 169, 383, 1475
NspI RCATGY 2 cut(s) 265, 574
NspV TTCGAA 2 cut(s) 12, 1009
OliI CACNNNNGTG 1 cut(s) 93
PagI TCATGA 1 cut(s) 352
PciI ACATGT 1 cut(s) 570
PciSI GCTCTTC 1 cut(s) 1036
PdmI GAANNNNTTC 2 cut(s) 615, 1115
PfeI GAWTC 3 cut(s) 290, 503, 1200
PkrI GCNGC 2 cut(s) 593, 658
PleI GAGTC 2 cut(s) 457, 502
PpsI GAGTC 2 cut(s) 457, 502
PscI ACATGT 1 cut(s) 570
PshBI ATTAAT 1 cut(s) 1464
PsiI TTATAA 1 cut(s) 767
Psp124BI GAGCTC 1 cut(s) 1048
PspFI CCCAGC 2 cut(s) 856, 1534
PspN4I GGNNCC 3 cut(s) 868, 1333, 1456
PspPI GGNCC 3 cut(s) 98, 1454, 1508
RsaI GTAC 2 cut(s) 207, 1061
RsaNI GTAC 2 cut(s) 206, 1060
RseI CAYNNNNRTG 3 cut(s) 93, 357, 461
SacI GAGCTC 1 cut(s) 1048
SacII CCGCGG 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 1036
SaqAI TTAA 3 cut(s) 825, 885, 1464
SatI GCNGC 2 cut(s) 592, 657
Sau3AI GATC 3 cut(s) 1192, 1297, 1428
Sau96I GGNCC 3 cut(s) 98, 1454, 1508
ScaI AGTACT 1 cut(s) 1061
SchI GAGTC 2 cut(s) 458, 502
SduI GDGCHC 2 cut(s) 1048, 1334
SfaNI GCATC 1 cut(s) 1459
SfcI CTRYAG 2 cut(s) 1402, 1503
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 12, 1009
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 3 cut(s) 98, 1454, 1508
SmiMI CAYNNNNRTG 3 cut(s) 93, 357, 461
SmlI CTYRAG 2 cut(s) 794, 1556
SmoI CTYRAG 2 cut(s) 794, 1556
SpeI ACTAGT 1 cut(s) 154
SsiI CCGC 4 cut(s) 82, 84, 101, 592
SspI AATATT 2 cut(s) 400, 1450
SspMI CTAG 7 cut(s) 155, 380, 1151, 1238, 1326, 1473, 1515
SstI GAGCTC 1 cut(s) 1048
StyI CCWWGG 6 cut(s) 285, 379, 1120, 1183, 1293, 1335
TaaI ACNGT 4 cut(s) 110, 134, 337, 880
TaiI ACGT 1 cut(s) 1005
TaqI TCGA 5 cut(s) 12, 279, 506, 644, 1009
TatI WGTACW 2 cut(s) 205, 1059
TauI GCSGC 1 cut(s) 594
TfiI GAWTC 3 cut(s) 290, 503, 1200
Tru1I TTAA 3 cut(s) 825, 885, 1464
Tru9I TTAA 3 cut(s) 825, 885, 1464
TscAI CASTG 3 cut(s) 115, 394, 427
TseFI GTSAC 3 cut(s) 169, 383, 1475
TseI GCWGC 1 cut(s) 656
Tsp45I GTSAC 3 cut(s) 169, 383, 1475
TspDTI ATGAA 8 cut(s) 135, 666, 756, 762, 855, 981, 1065, 1078
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 3 cut(s) 115, 394, 427
VpaK11BI GGWCC 3 cut(s) 98, 1454, 1508
VspI ATTAAT 1 cut(s) 1464
XapI RAATTY 6 cut(s) 161, 634, 842, 905, 1260, 1369
XceI RCATGY 2 cut(s) 265, 574
XmaJI CCTAGG 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 533
XmnI GAANNNNTTC 2 cut(s) 615, 1115
XspI CTAG 7 cut(s) 155, 380, 1151, 1238, 1326, 1473, 1515
ZrmI AGTACT 1 cut(s) 1061
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.