Rw5G029440

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000107
Physical Location & Seq
Reverse (-)
45242443 .. 45244480
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029440.1

Sequence Viewer

Length: 1785 bp
ATGCTTCTAACTATGAGTTTCTTCTTCTGGTTGCTCCTGATATTTGTAGATGTAGATGTTGTGCATGGTGGAGGGGTAGGTCGTAAAAATTGCACAGAAACAAGATGTAGCAGCGATGGCCCAGCTATCCGGTTCCCATTTCGACTGAAAGGTAGGCAACCGGTCCAATGTGGTTCCCCGGGCTTTGATCTTGCATGCACCAACGACAACCAGACACTACTTCAGATGCCATCATCATCTAAGCTCTTTGTTACAGAGATTAACTACAAATCACAGACAATTGAAGCAGAACTTGCACCTGATTGCTTGGACGGAGACATTTTCGACCTTGGTTCTTCTTCTAGCTTCAAATATGTAGGCAACGCAAGCTTGTTCAGTTGCCCACCATCAACTGTGAGAGATCAATATATTACAGCATATCCCTACAAGTACAATTCTCGTTGTCTAGCAAGATTGAGCCCTTGCCATAGTAATCCAGGCAACCAAATTTTTGCTTTTAGGACAGGTTGTTATTCTCTTGCATACACAGATGACTATATATTTGACGATATATATTCACGCGTGTCTCTCTACTGGTCCATACCATCTTGTAAACATTGTAAAGAAAAGGGCGAGCTATGTCAATTCACAAATCAGACAGATCCTCAAACTCAATGCTTGGATAGACCCAAAGCCAGAGCAGGGAACAAACGACTTGAATTTTCAATTACATTAGATCATGTCATAGCATGTATCGGACTTTTTATCACAGTTCCTTATCTTCTTATAGTGACTGGAACAATAATCTACTATCCCTTTTTCACTAAAAGAATAGAAAGTCAGCTGAGAATTAAAAGCTTTTTGGATGACTACAGAGCTCTTAAGCCAAGCAGATATTCTTATGCAGATATTAAGAGGATAACAGAGCAGTTCAAGGAGAAGCTGGGTCAAGGGGCCTATGGAACTGTCTTTAAAGGTAAACTTTCCTCTAAATTACTTGTTGCTGTTAAAATCCTCAACAATTCAAATGAGAAAGGGGATGATTTTATTAATGAAGTGGGCACAATGAGTCAAATCCACCATGTCAATGTGGTACGCTTGGTTGGTTACTGTGCTGATGGCTTTATACGAGCTCTTGTTTATGAATTCTTACCAAACGGTCCACTCCAGAATTTCTTATCATCAGCAGATAATGAGAGTTCGTTCCTTGGTTGGGATAAGTTGCAACATATTGCTTTAGGTATAGCCAAAGGAATTGAATATATTCACCAAGGTTGTGAACAACAAATCCTCCACTTTGACATCAAACCCCATAATGTGTTGCTAGACCATGATTTCACTCCAAAAGTTTCTGATTTTGGTTTAGCCAAGTTGTGCTCTAAGGATCAAAGTGTGGTATCCATGACTGCAGCGAGGGGAACCATGGGCTACATTGCACCAGAAGTCTTCTCTAGGAACTTCGGTAAGGTGTCATATAAGGCAGATGTCTATAGTTTTGGAACATTGCTTCTTGAAATGGTTGGGGGTAGAAAGAATTTTAAAGTTATGGAAGACTCCACCAGCCAGGTCTACTTCCCAGAATGGATCTATAACCTCTTAGAACAAGGGAACGACCTTCGCATCCATATTGGAGACGAAGGAAATGTTGAAATTGCTAAGAAACTTGCGGTTGTGGGTCTATGGTGCATCCAATGGTATCCAATAGACCGTCCTTCCATGAAAACAGTAGTTCAAATGTTGGAAAGGGAAGGTGACAATCTGACCATGCCTCCTAATCCTTTTGCCTCTACTTCATCTTCGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

594

Amino Acids

66.83

Weight (kDa)

7.12

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 95 1.4e-17 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 303 - 573 5.6e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 304 - 571 1e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1548
AccII CGCG 1 cut(s) 561
AciI CCGC 1 cut(s) 1646
AclWI GGATC 3 cut(s) 635, 1371, 1571
AcsI RAATTY 5 cut(s) 486, 698, 1124, 1150, 1513
AcuI CTGAAG 1 cut(s) 206
AfaI GTAC 2 cut(s) 431, 1074
AfiI CCNNNNNNNGG 2 cut(s) 681, 1192
AflII CTTAAG 1 cut(s) 860
AflIII ACRYGT 1 cut(s) 559
AgeI ACCGGT 1 cut(s) 160
AjnI CCWGG 2 cut(s) 475, 1542
AjuI GAANNNNNNNTTGG 2 cut(s) 859, 891
AloI GAACNNNNNNTCC 2 cut(s) 1251, 1283
Alw21I GWGCWC 3 cut(s) 859, 1114, 1358
Alw26I GTCTC 3 cut(s) 309, 570, 1605
AlwI GGATC 3 cut(s) 635, 1371, 1571
Ama87I CYCGRG 1 cut(s) 178
AoxI GGCC 2 cut(s) 118, 933
ApeKI GCWGC 2 cut(s) 111, 1388
ApoI RAATTY 5 cut(s) 486, 698, 1124, 1150, 1513
ArsI GACNNNNNNTTYG 2 cut(s) 524, 556
AseI ATTAAT 1 cut(s) 1029
AsiGI ACCGGT 1 cut(s) 160
Asp700I GAANNNNTTC 1 cut(s) 1242
AspS9I GGNCC 5 cut(s) 119, 163, 576, 933, 1139
AsuC2I CCSGG 2 cut(s) 179, 180
AsuHPI GGTGA 2 cut(s) 1238, 1742
AvaI CYCGRG 1 cut(s) 178
AvaII GGWCC 3 cut(s) 163, 576, 1139
BaeGI GKGCMC 1 cut(s) 1043
BanII GRGCYC 3 cut(s) 461, 859, 1114
BbsI GAAGAC 2 cut(s) 1417, 1536
Bbv12I GWGCWC 3 cut(s) 859, 1114, 1358
BbvI GCAGC 2 cut(s) 123, 1400
BccI CCATC 5 cut(s) 110, 238, 394, 592, 1091
BciT130I CCWGG 2 cut(s) 477, 1544
BciVI GTATCC 2 cut(s) 1387, 1686
BcnI CCSGG 2 cut(s) 179, 180
BcoDI GTCTC 3 cut(s) 309, 570, 1605
BfaI CTAG 4 cut(s) 342, 446, 1304, 1431
BfmI CTRYAG 3 cut(s) 850, 1386, 1468
BfrI CTTAAG 1 cut(s) 860
BfuI GTATCC 2 cut(s) 1387, 1686
BisI GCNGC 2 cut(s) 112, 1389
BlsI GCNGC 2 cut(s) 113, 1390
Bme1390I CCNGG 4 cut(s) 179, 180, 477, 1544
Bme18I GGWCC 3 cut(s) 163, 576, 1139
BmeT110I CYCGRG 1 cut(s) 178
BmgT120I GGNCC 5 cut(s) 119, 163, 576, 933, 1139
BmiI GGNNCC 4 cut(s) 134, 175, 934, 1399
BmrFI CCNGG 4 cut(s) 179, 180, 477, 1544
BmsI GCATC 3 cut(s) 216, 1608, 1674
BpiI GAAGAC 2 cut(s) 1417, 1536
BpmI CTGGAG 1 cut(s) 1130
BpuMI CCSGG 2 cut(s) 179, 180
BsaJI CCNNGG 6 cut(s) 177, 178, 328, 1186, 1249, 1401
BsaWI WCCGGW 2 cut(s) 129, 160
BsaXI ACNNNNNCTCC 4 cut(s) 1254, 1284, 1732, 1762
Bsc4I CCNNNNNNNGG 2 cut(s) 681, 1192
Bse118I RCCGGY 1 cut(s) 160
Bse1I ACTGG 2 cut(s) 578, 778
Bse3DI GCAATG 2 cut(s) 1410, 1481
BseBI CCWGG 2 cut(s) 477, 1544
BseDI CCNNGG 6 cut(s) 177, 178, 328, 1186, 1249, 1401
BseGI GGATG 4 cut(s) 850, 1024, 1599, 1665
BseLI CCNNNNNNNGG 2 cut(s) 681, 1192
BseMI GCAATG 2 cut(s) 1410, 1481
BseMII CTCAG 1 cut(s) 815
BseNI ACTGG 2 cut(s) 578, 778
BseSI GKGCMC 1 cut(s) 1043
BseXI GCAGC 2 cut(s) 123, 1400
BseYI CCCAGC 2 cut(s) 121, 922
Bsh1236I CGCG 1 cut(s) 561
BshFI GGCC 2 cut(s) 120, 935
BshTI ACCGGT 1 cut(s) 160
BsiHKAI GWGCWC 3 cut(s) 859, 1114, 1358
BsiHKCI CYCGRG 1 cut(s) 178
BsiSI CCGG 3 cut(s) 130, 161, 179
BslI CCNNNNNNNGG 2 cut(s) 681, 1192
BsmAI GTCTC 3 cut(s) 309, 570, 1605
BsmBI CGTCTC 1 cut(s) 1605
BsnI GGCC 2 cut(s) 120, 935
BsoBI CYCGRG 1 cut(s) 178
Bsp1286I GDGCHC 5 cut(s) 461, 859, 1043, 1114, 1358
Bsp143I GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 1 cut(s) 1646
BspANI GGCC 2 cut(s) 120, 935
BspCNI CTCAG 1 cut(s) 816
BspFNI CGCG 1 cut(s) 561
BspLI GGNNCC 4 cut(s) 134, 175, 934, 1399
BspMAI CTGCAG 1 cut(s) 1390
BspPI GGATC 3 cut(s) 635, 1371, 1571
BspTI CTTAAG 1 cut(s) 860
BsrDI GCAATG 2 cut(s) 1410, 1481
BsrFI RCCGGY 1 cut(s) 160
BsrI ACTGG 2 cut(s) 578, 778
BssAI RCCGGY 1 cut(s) 160
BssECI CCNNGG 6 cut(s) 177, 178, 328, 1186, 1249, 1401
BssMI GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
BssT1I CCWWGG 4 cut(s) 328, 1186, 1249, 1401
Bst2UI CCWGG 2 cut(s) 477, 1544
Bst4CI ACNGT 7 cut(s) 394, 751, 946, 1091, 1139, 1688, 1705
BstAFI CTTAAG 1 cut(s) 860
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 3 cut(s) 196, 367, 614
BstDEI CTNAG 5 cut(s) 240, 824, 1359, 1576, 1635
BstDSI CCRYGG 1 cut(s) 1401
BstF5I GGATG 4 cut(s) 850, 1024, 1599, 1665
BstFNI CGCG 1 cut(s) 561
BstKTI GATC 6 cut(s) 190, 403, 643, 718, 1366, 1566
BstMAI GTCTC 3 cut(s) 309, 570, 1605
BstMBI GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
BstMWI GCNNNNNNNGC 3 cut(s) 117, 293, 366
BstNI CCWGG 2 cut(s) 477, 1544
BstNSI RCATGY 2 cut(s) 198, 732
BstSCI CCNGG 4 cut(s) 177, 178, 475, 1542
BstSFI CTRYAG 3 cut(s) 850, 1386, 1468
BstSLI GKGCMC 1 cut(s) 1043
BstUI CGCG 1 cut(s) 561
BstV1I GCAGC 2 cut(s) 123, 1400
BstV2I GAAGAC 2 cut(s) 1417, 1536
BstX2I RGATCY 2 cut(s) 640, 1563
BstYI RGATCY 2 cut(s) 640, 1563
BsuI GTATCC 2 cut(s) 1387, 1686
BsuRI GGCC 2 cut(s) 120, 935
BtgI CCRYGG 1 cut(s) 1401
BtgZI GCGATG 1 cut(s) 129
BtsCI GGATG 4 cut(s) 850, 1024, 1599, 1665
Cac8I GCNNGC 3 cut(s) 196, 367, 614
Cfr10I RCCGGY 1 cut(s) 160
Cfr13I GGNCC 5 cut(s) 119, 163, 576, 933, 1139
Cfr9I CCCGGG 1 cut(s) 178
Csp6I GTAC 2 cut(s) 430, 1073
CspAI ACCGGT 1 cut(s) 160
CviQI GTAC 2 cut(s) 430, 1073
DdeI CTNAG 5 cut(s) 240, 824, 1359, 1576, 1635
DpnI GATC 6 cut(s) 189, 402, 642, 717, 1365, 1565
DpnII GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
DraI TTTAAA 2 cut(s) 952, 1519
Ecl136II GAGCTC 2 cut(s) 857, 1112
Eco130I CCWWGG 4 cut(s) 328, 1186, 1249, 1401
Eco24I GRGCYC 3 cut(s) 461, 859, 1114
Eco47I GGWCC 3 cut(s) 163, 576, 1139
Eco53kI GAGCTC 2 cut(s) 857, 1112
Eco57I CTGAAG 1 cut(s) 206
Eco88I CYCGRG 1 cut(s) 178
EcoICRI GAGCTC 2 cut(s) 857, 1112
EcoO109I RGGNCCY 1 cut(s) 933
EcoRI GAATTC 1 cut(s) 1124
EcoRII CCWGG 2 cut(s) 475, 1542
EcoT14I CCWWGG 4 cut(s) 328, 1186, 1249, 1401
EcoT38I GRGCYC 3 cut(s) 461, 859, 1114
ErhI CCWWGG 4 cut(s) 328, 1186, 1249, 1401
Esp3I CGTCTC 1 cut(s) 1605
FalI AAGNNNNNCTT 4 cut(s) 276, 308, 945, 977
FblI GTMKAC 1 cut(s) 1548
Fnu4HI GCNGC 2 cut(s) 112, 1389
FokI GGATG 4 cut(s) 857, 1031, 1586, 1652
FriOI GRGCYC 3 cut(s) 461, 859, 1114
Fsp4HI GCNGC 2 cut(s) 112, 1389
FspBI CTAG 4 cut(s) 342, 446, 1304, 1431
GluI GCNGC 2 cut(s) 112, 1389
GsaI CCCAGC 2 cut(s) 125, 926
GsuI CTGGAG 1 cut(s) 1130
HaeIII GGCC 2 cut(s) 120, 935
HapII CCGG 3 cut(s) 130, 161, 179
HindIII AAGCTT 2 cut(s) 367, 835
HinfI GANTC 2 cut(s) 1048, 1532
HpaII CCGG 3 cut(s) 130, 161, 179
HphI GGTGA 2 cut(s) 1238, 1742
Hpy166II GTNNAC 5 cut(s) 593, 959, 1142, 1259, 1549
Hpy188I TCNGA 5 cut(s) 225, 636, 737, 1333, 1740
Hpy188III TCNNGA 3 cut(s) 37, 1147, 1490
Hpy8I GTNNAC 5 cut(s) 593, 959, 1142, 1259, 1549
HpyAV CCTTC 4 cut(s) 1604, 1610, 1701, 1721
HpyCH4III ACNGT 7 cut(s) 394, 751, 946, 1091, 1139, 1688, 1705
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 293, 366
HpyF3I CTNAG 5 cut(s) 240, 824, 1359, 1576, 1635
Kzo9I GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
LmnI GCTCC 1 cut(s) 39
Lsp1109I GCAGC 2 cut(s) 123, 1400
LweI GCATC 3 cut(s) 216, 1608, 1674
MaeI CTAG 4 cut(s) 342, 446, 1304, 1431
MaeIII GTNAC 4 cut(s) 250, 769, 1085, 1730
MalI GATC 6 cut(s) 189, 402, 642, 717, 1365, 1565
MboI GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
MboII GAAGA 8 cut(s) 13, 16, 327, 330, 752, 1417, 1541, 1767
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 2 cut(s) 640, 1563
MhlI GDGCHC 5 cut(s) 461, 859, 1043, 1114, 1358
MluI ACGCGT 1 cut(s) 559
MlyI GAGTC 2 cut(s) 1057, 1526
MmeI TCCRAC 1 cut(s) 1698
MroXI GAANNNNTTC 1 cut(s) 1242
MseI TTAA 8 cut(s) 261, 831, 861, 891, 951, 987, 1029, 1518
MslI CAYNNNNRTG 1 cut(s) 1065
MspA1I CMGCKG 1 cut(s) 823
MspCI CTTAAG 1 cut(s) 860
MspI CCGG 3 cut(s) 130, 161, 179
MspR9I CCNGG 4 cut(s) 179, 180, 477, 1544
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 2 cut(s) 477, 1544
MvnI CGCG 1 cut(s) 561
MwoI GCNNNNNNNGC 3 cut(s) 117, 293, 366
NciI CCSGG 2 cut(s) 179, 180
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
NlaIV GGNNCC 4 cut(s) 134, 175, 934, 1399
NmuCI GTSAC 2 cut(s) 769, 1730
NspI RCATGY 2 cut(s) 198, 732
PaeI GCATGC 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 1242
PinAI ACCGGT 1 cut(s) 160
PkrI GCNGC 2 cut(s) 113, 1390
PleI GAGTC 2 cut(s) 1056, 1526
PpsI GAGTC 2 cut(s) 1056, 1526
PshBI ATTAAT 1 cut(s) 1029
Psp124BI GAGCTC 2 cut(s) 859, 1114
Psp6I CCWGG 2 cut(s) 475, 1542
PspFI CCCAGC 2 cut(s) 121, 922
PspGI CCWGG 2 cut(s) 475, 1542
PspN4I GGNNCC 4 cut(s) 134, 175, 934, 1399
PspPI GGNCC 5 cut(s) 119, 163, 576, 933, 1139
PstI CTGCAG 1 cut(s) 1390
PsuI RGATCY 2 cut(s) 640, 1563
PvuII CAGCTG 1 cut(s) 823
RsaI GTAC 2 cut(s) 431, 1074
RsaNI GTAC 2 cut(s) 430, 1073
RseI CAYNNNNRTG 1 cut(s) 1065
SacI GAGCTC 2 cut(s) 859, 1114
SaqAI TTAA 8 cut(s) 261, 831, 861, 891, 951, 987, 1029, 1518
SatI GCNGC 2 cut(s) 112, 1389
Sau3AI GATC 6 cut(s) 187, 400, 640, 715, 1363, 1563
Sau96I GGNCC 5 cut(s) 119, 163, 576, 933, 1139
SchI GAGTC 2 cut(s) 1057, 1526
ScrFI CCNGG 4 cut(s) 179, 180, 477, 1544
SduI GDGCHC 5 cut(s) 461, 859, 1043, 1114, 1358
SfaNI GCATC 3 cut(s) 216, 1608, 1674
SfcI CTRYAG 3 cut(s) 850, 1386, 1468
SinI GGWCC 3 cut(s) 163, 576, 1139
SmaI CCCGGG 1 cut(s) 180
SmiMI CAYNNNNRTG 1 cut(s) 1065
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SphI GCATGC 1 cut(s) 198
SsiI CCGC 1 cut(s) 1646
SspMI CTAG 4 cut(s) 342, 446, 1304, 1431
SstI GAGCTC 2 cut(s) 859, 1114
StyD4I CCNGG 4 cut(s) 177, 178, 475, 1542
StyI CCWWGG 4 cut(s) 328, 1186, 1249, 1401
TaaI ACNGT 7 cut(s) 394, 751, 946, 1091, 1139, 1688, 1705
TaqI TCGA 3 cut(s) 142, 324, 1778
TatI WGTACW 1 cut(s) 429
Tru1I TTAA 8 cut(s) 261, 831, 861, 891, 951, 987, 1029, 1518
Tru9I TTAA 8 cut(s) 261, 831, 861, 891, 951, 987, 1029, 1518
TseFI GTSAC 2 cut(s) 769, 1730
TseI GCWGC 2 cut(s) 111, 1388
Tsp45I GTSAC 2 cut(s) 769, 1730
TspDTI ATGAA 4 cut(s) 1047, 1137, 1712, 1761
TspGWI ACGGA 1 cut(s) 327
TspMI CCCGGG 1 cut(s) 178
Vha464I CTTAAG 1 cut(s) 860
VpaK11BI GGWCC 3 cut(s) 163, 576, 1139
VspI ATTAAT 1 cut(s) 1029
XapI RAATTY 5 cut(s) 486, 698, 1124, 1150, 1513
XceI RCATGY 2 cut(s) 198, 732
XmaI CCCGGG 1 cut(s) 178
XmiI GTMKAC 1 cut(s) 1548
XmnI GAANNNNTTC 1 cut(s) 1242
XspI CTAG 4 cut(s) 342, 446, 1304, 1431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.