receptor-like protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1608 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 13 |
| Acc36I | ACCTGC | 1 cut(s) | 1796 |
| AccB1I | GGYRCC | 3 cut(s) | 695, 1713, 1845 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 262 |
| AccII | CGCG | 2 cut(s) | 195, 822 |
| AciI | CCGC | 7 cut(s) | 153, 190, 193, 195, 234, 245, 1130 |
| AclWI | GGATC | 2 cut(s) | 1407, 1731 |
| AcoI | YGGCCR | 3 cut(s) | 484, 756, 1380 |
| AcsI | RAATTY | 4 cut(s) | 26, 275, 1471, 1584 |
| AcyI | GRCGYC | 2 cut(s) | 507, 1846 |
| AdeI | CACNNNGTG | 1 cut(s) | 1720 |
| AfaI | GTAC | 5 cut(s) | 267, 408, 559, 953, 1029 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 262, 479, 1228, 1618, 1648, 1858 |
| AhlI | ACTAGT | 1 cut(s) | 268 |
| AjiI | CACGTC | 1 cut(s) | 1099 |
| AjnI | CCWGG | 4 cut(s) | 168, 777, 1182, 1466 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 895, 927 |
| AluBI | AGCT | 3 cut(s) | 48, 893, 1148 |
| AluI | AGCT | 3 cut(s) | 48, 893, 1148 |
| Alw21I | GWGCWC | 2 cut(s) | 140, 1150 |
| Alw26I | GTCTC | 1 cut(s) | 514 |
| Alw44I | GTGCAC | 1 cut(s) | 136 |
| AlwI | GGATC | 2 cut(s) | 1407, 1731 |
| AoxI | GGCC | 6 cut(s) | 99, 166, 484, 756, 961, 1380 |
| ApaLI | GTGCAC | 1 cut(s) | 136 |
| ApeKI | GCWGC | 3 cut(s) | 58, 1519, 1522 |
| ApoI | RAATTY | 4 cut(s) | 26, 275, 1471, 1584 |
| Asp700I | GAANNNNTTC | 4 cut(s) | 717, 997, 1217, 1278 |
| AspLEI | GCGC | 1 cut(s) | 1848 |
| AspS9I | GGNCC | 3 cut(s) | 633, 1434, 1808 |
| AsuC2I | CCSGG | 1 cut(s) | 208 |
| AsuHPI | GGTGA | 4 cut(s) | 317, 355, 713, 1085 |
| AvaII | GGWCC | 3 cut(s) | 633, 1434, 1808 |
| BaeGI | GKGCMC | 1 cut(s) | 140 |
| BalI | TGGCCA | 3 cut(s) | 486, 758, 1382 |
| BanI | GGYRCC | 3 cut(s) | 695, 1713, 1845 |
| BanII | GRGCYC | 2 cut(s) | 1150, 1698 |
| Bbv12I | GWGCWC | 2 cut(s) | 140, 1150 |
| BbvI | GCAGC | 3 cut(s) | 70, 1506, 1509 |
| BccI | CCATC | 3 cut(s) | 1043, 1127, 1655 |
| BciT130I | CCWGG | 4 cut(s) | 170, 779, 1184, 1468 |
| BciVI | GTATCC | 1 cut(s) | 485 |
| BclI | TGATCA | 3 cut(s) | 66, 160, 835 |
| BcnI | CCSGG | 1 cut(s) | 208 |
| BcoDI | GTCTC | 1 cut(s) | 514 |
| BcuI | ACTAGT | 1 cut(s) | 268 |
| BfaI | CTAG | 8 cut(s) | 9, 35, 269, 800, 1340, 1616, 1757, 1853 |
| BfmI | CTRYAG | 4 cut(s) | 548, 826, 1422, 1803 |
| BfoI | RGCGCY | 1 cut(s) | 1849 |
| BfuAI | ACCTGC | 1 cut(s) | 1796 |
| BfuI | GTATCC | 1 cut(s) | 485 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 483 |
| BisI | GCNGC | 5 cut(s) | 59, 154, 193, 1520, 1523 |
| BlsI | GCNGC | 5 cut(s) | 60, 155, 194, 1521, 1524 |
| Bme1390I | CCNGG | 5 cut(s) | 170, 208, 779, 1184, 1468 |
| Bme18I | GGWCC | 3 cut(s) | 633, 1434, 1808 |
| BmgBI | CACGTC | 1 cut(s) | 1099 |
| BmgT120I | GGNCC | 3 cut(s) | 633, 1434, 1808 |
| BmiI | GGNNCC | 6 cut(s) | 635, 697, 970, 1435, 1715, 1847 |
| BmrFI | CCNGG | 5 cut(s) | 170, 208, 779, 1184, 1468 |
| BmrI | ACTGGG | 1 cut(s) | 1712 |
| BmsI | GCATC | 8 cut(s) | 45, 208, 313, 1280, 1713, 1743, 1770, 1842 |
| BmuI | ACTGGG | 1 cut(s) | 1712 |
| BpmI | CTGGAG | 1 cut(s) | 1166 |
| BpuEI | CTTGAG | 2 cut(s) | 881, 1547 |
| BpuMI | CCSGG | 1 cut(s) | 208 |
| Bsa29I | ATCGAT | 1 cut(s) | 856 |
| BsaAI | YACGTR | 1 cut(s) | 410 |
| BsaHI | GRCGYC | 2 cut(s) | 507, 1846 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1290, 1320, 1768, 1798 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 262, 479, 1228, 1618, 1648, 1858 |
| Bse1I | ACTGG | 2 cut(s) | 1708, 1718 |
| Bse3DI | GCAATG | 4 cut(s) | 235, 1086, 1245, 1446 |
| BseBI | CCWGG | 4 cut(s) | 170, 779, 1184, 1468 |
| BseCI | ATCGAT | 1 cut(s) | 856 |
| BseGI | GGATG | 6 cut(s) | 570, 1138, 1295, 1666, 1704, 1833 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 262, 479, 1228, 1618, 1648, 1858 |
| BseMI | GCAATG | 4 cut(s) | 235, 1086, 1245, 1446 |
| BseNI | ACTGG | 2 cut(s) | 1708, 1718 |
| BseRI | GAGGAG | 1 cut(s) | 1779 |
| BseSI | GKGCMC | 1 cut(s) | 140 |
| BseXI | GCAGC | 3 cut(s) | 70, 1506, 1509 |
| Bsh1236I | CGCG | 2 cut(s) | 195, 822 |
| BshFI | GGCC | 6 cut(s) | 101, 168, 486, 758, 963, 1382 |
| BshNI | GGYRCC | 3 cut(s) | 695, 1713, 1845 |
| BshVI | ATCGAT | 1 cut(s) | 856 |
| BsiHKAI | GWGCWC | 2 cut(s) | 140, 1150 |
| BsiSI | CCGG | 1 cut(s) | 207 |
| BslFI | GGGAC | 4 cut(s) | 455, 619, 1244, 1447 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 262, 479, 1228, 1618, 1648, 1858 |
| BsmAI | GTCTC | 1 cut(s) | 514 |
| BsmBI | CGTCTC | 1 cut(s) | 514 |
| BsmFI | GGGAC | 4 cut(s) | 455, 619, 1244, 1447 |
| BsmI | GAATGC | 2 cut(s) | 1013, 1521 |
| BsnI | GGCC | 6 cut(s) | 101, 168, 486, 758, 963, 1382 |
| Bsp1286I | GDGCHC | 3 cut(s) | 140, 1150, 1698 |
| Bsp1407I | TGTACA | 1 cut(s) | 557 |
| Bsp143I | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| Bsp19I | CCATGG | 2 cut(s) | 487, 1437 |
| BspACI | CCGC | 7 cut(s) | 153, 190, 193, 195, 234, 245, 1130 |
| BspANI | GGCC | 6 cut(s) | 101, 168, 486, 758, 963, 1382 |
| BspDI | ATCGAT | 1 cut(s) | 856 |
| BspFNI | CGCG | 2 cut(s) | 195, 822 |
| BspLI | GGNNCC | 6 cut(s) | 635, 697, 970, 1435, 1715, 1847 |
| BspMAI | CTGCAG | 1 cut(s) | 1807 |
| BspMI | ACCTGC | 1 cut(s) | 1796 |
| BspPI | GGATC | 2 cut(s) | 1407, 1731 |
| BspT107I | GGYRCC | 3 cut(s) | 695, 1713, 1845 |
| BsrDI | GCAATG | 4 cut(s) | 235, 1086, 1245, 1446 |
| BsrGI | TGTACA | 1 cut(s) | 557 |
| BsrI | ACTGG | 2 cut(s) | 1708, 1718 |
| BssMI | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| BssNI | GRCGYC | 2 cut(s) | 507, 1846 |
| BssT1I | CCWWGG | 6 cut(s) | 478, 487, 964, 1222, 1395, 1437 |
| Bst2UI | CCWGG | 4 cut(s) | 170, 779, 1184, 1468 |
| Bst4CI | ACNGT | 5 cut(s) | 373, 552, 763, 982, 1744 |
| Bst6I | CTCTTC | 3 cut(s) | 79, 517, 1050 |
| BstACI | GRCGYC | 2 cut(s) | 507, 1846 |
| BstAUI | TGTACA | 1 cut(s) | 557 |
| BstBAI | YACGTR | 1 cut(s) | 410 |
| BstC8I | GCNNGC | 4 cut(s) | 136, 197, 341, 1831 |
| BstDEI | CTNAG | 1 cut(s) | 1041 |
| BstDSI | CCRYGG | 3 cut(s) | 193, 487, 1437 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1616 |
| BstF5I | GGATG | 6 cut(s) | 570, 1138, 1295, 1666, 1704, 1833 |
| BstFNI | CGCG | 2 cut(s) | 195, 822 |
| BstH2I | RGCGCY | 1 cut(s) | 1849 |
| BstHHI | GCGC | 1 cut(s) | 1848 |
| BstKTI | GATC | 6 cut(s) | 69, 163, 838, 1297, 1402, 1726 |
| BstMAI | GTCTC | 1 cut(s) | 514 |
| BstMBI | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 45, 349, 483, 702, 764 |
| BstNI | CCWGG | 4 cut(s) | 170, 779, 1184, 1468 |
| BstSCI | CCNGG | 5 cut(s) | 168, 206, 777, 1182, 1466 |
| BstSFI | CTRYAG | 4 cut(s) | 548, 826, 1422, 1803 |
| BstSLI | GKGCMC | 1 cut(s) | 140 |
| BstSNI | TACGTA | 1 cut(s) | 410 |
| BstUI | CGCG | 2 cut(s) | 195, 822 |
| BstV1I | GCAGC | 3 cut(s) | 70, 1506, 1509 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1270 |
| Bsu15I | ATCGAT | 1 cut(s) | 856 |
| BsuI | GTATCC | 1 cut(s) | 485 |
| BsuRI | GGCC | 6 cut(s) | 101, 168, 486, 758, 963, 1382 |
| BsuTUI | ATCGAT | 1 cut(s) | 856 |
| BtgI | CCRYGG | 3 cut(s) | 193, 487, 1437 |
| BtgZI | GCGATG | 2 cut(s) | 171, 1062 |
| BtrI | CACGTC | 1 cut(s) | 1099 |
| BtsCI | GGATG | 6 cut(s) | 570, 1138, 1295, 1666, 1704, 1833 |
| BtsIMutI | CAGTG | 2 cut(s) | 1715, 1725 |
| BveI | ACCTGC | 1 cut(s) | 1796 |
| Cac8I | GCNNGC | 4 cut(s) | 136, 197, 341, 1831 |
| CfoI | GCGC | 1 cut(s) | 1848 |
| Cfr13I | GGNCC | 3 cut(s) | 633, 1434, 1808 |
| Cfr42I | CCGCGG | 1 cut(s) | 196 |
| ClaI | ATCGAT | 1 cut(s) | 856 |
| CseI | GACGC | 2 cut(s) | 496, 811 |
| Csp6I | GTAC | 5 cut(s) | 266, 407, 558, 952, 1028 |
| CviAII | CATG | 4 cut(s) | 164, 488, 1438, 1735 |
| CviQI | GTAC | 5 cut(s) | 266, 407, 558, 952, 1028 |
| DdeI | CTNAG | 1 cut(s) | 1041 |
| DinI | GGCGCC | 1 cut(s) | 1847 |
| DpnI | GATC | 6 cut(s) | 68, 162, 837, 1296, 1401, 1725 |
| DpnII | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| DraI | TTTAAA | 1 cut(s) | 988 |
| DraIII | CACNNNGTG | 1 cut(s) | 1720 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 13 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 13 |
| EaeI | YGGCCR | 3 cut(s) | 484, 756, 1380 |
| Eam1104I | CTCTTC | 3 cut(s) | 79, 517, 1050 |
| EarI | CTCTTC | 3 cut(s) | 79, 517, 1050 |
| EciI | GGCGGA | 2 cut(s) | 179, 249 |
| Ecl136II | GAGCTC | 1 cut(s) | 1148 |
| Eco105I | TACGTA | 1 cut(s) | 410 |
| Eco130I | CCWWGG | 6 cut(s) | 478, 487, 964, 1222, 1395, 1437 |
| Eco24I | GRGCYC | 2 cut(s) | 1150, 1698 |
| Eco32I | GATATC | 1 cut(s) | 1318 |
| Eco47I | GGWCC | 3 cut(s) | 633, 1434, 1808 |
| Eco53kI | GAGCTC | 1 cut(s) | 1148 |
| EcoICRI | GAGCTC | 1 cut(s) | 1148 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1616 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1808 |
| EcoRII | CCWGG | 4 cut(s) | 168, 777, 1182, 1466 |
| EcoRV | GATATC | 1 cut(s) | 1318 |
| EcoT14I | CCWWGG | 6 cut(s) | 478, 487, 964, 1222, 1395, 1437 |
| EcoT38I | GRGCYC | 2 cut(s) | 1150, 1698 |
| EgeI | GGCGCC | 1 cut(s) | 1847 |
| EheI | GGCGCC | 1 cut(s) | 1847 |
| ErhI | CCWWGG | 6 cut(s) | 478, 487, 964, 1222, 1395, 1437 |
| Esp3I | CGTCTC | 1 cut(s) | 514 |
| FaeI | CATG | 4 cut(s) | 167, 491, 1441, 1738 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 981, 1013 |
| FaqI | GGGAC | 4 cut(s) | 455, 619, 1244, 1447 |
| FatI | CATG | 4 cut(s) | 163, 487, 1437, 1734 |
| FauI | CCCGC | 1 cut(s) | 188 |
| FauNDI | CATATG | 1 cut(s) | 916 |
| FbaI | TGATCA | 3 cut(s) | 66, 160, 835 |
| Fnu4HI | GCNGC | 5 cut(s) | 59, 154, 193, 1520, 1523 |
| FokI | GGATG | 6 cut(s) | 577, 1145, 1302, 1673, 1691, 1820 |
| FriOI | GRGCYC | 2 cut(s) | 1150, 1698 |
| Fsp4HI | GCNGC | 5 cut(s) | 59, 154, 193, 1520, 1523 |
| FspBI | CTAG | 8 cut(s) | 9, 35, 269, 800, 1340, 1616, 1757, 1853 |
| GlaI | GCGC | 1 cut(s) | 1847 |
| GluI | GCNGC | 5 cut(s) | 59, 154, 193, 1520, 1523 |
| GsuI | CTGGAG | 1 cut(s) | 1166 |
| HaeII | RGCGCY | 1 cut(s) | 1849 |
| HaeIII | GGCC | 6 cut(s) | 101, 168, 486, 758, 963, 1382 |
| HapII | CCGG | 1 cut(s) | 207 |
| HgaI | GACGC | 2 cut(s) | 496, 811 |
| HhaI | GCGC | 1 cut(s) | 1848 |
| Hin1I | GRCGYC | 2 cut(s) | 507, 1846 |
| Hin1II | CATG | 4 cut(s) | 167, 491, 1441, 1738 |
| Hin6I | GCGC | 1 cut(s) | 1846 |
| HinP1I | GCGC | 1 cut(s) | 1846 |
| HinfI | GANTC | 6 cut(s) | 14, 143, 853, 1302, 1638, 1669 |
| HpaII | CCGG | 1 cut(s) | 207 |
| HphI | GGTGA | 4 cut(s) | 317, 355, 713, 1085 |
| Hpy166II | GTNNAC | 2 cut(s) | 138, 721 |
| Hpy188I | TCNGA | 3 cut(s) | 1006, 1369, 1752 |
| Hpy188III | TCNNGA | 5 cut(s) | 18, 251, 308, 1595, 1616 |
| Hpy8I | GTNNAC | 2 cut(s) | 138, 721 |
| HpyCH4III | ACNGT | 5 cut(s) | 373, 552, 763, 982, 1744 |
| HpyCH4IV | ACGT | 3 cut(s) | 409, 749, 1098 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 45, 349, 483, 702, 764 |
| HpyF3I | CTNAG | 1 cut(s) | 1041 |
| HpySE526I | ACGT | 3 cut(s) | 409, 749, 1098 |
| Hsp92I | GRCGYC | 2 cut(s) | 507, 1846 |
| Hsp92II | CATG | 4 cut(s) | 167, 491, 1441, 1738 |
| HspAI | GCGC | 1 cut(s) | 1846 |
| KasI | GGCGCC | 1 cut(s) | 1845 |
| Ksp22I | TGATCA | 3 cut(s) | 66, 160, 835 |
| KspI | CCGCGG | 1 cut(s) | 196 |
| Kzo9I | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| LmnI | GCTCC | 4 cut(s) | 770, 968, 1076, 1185 |
| Lsp1109I | GCAGC | 3 cut(s) | 70, 1506, 1509 |
| LweI | GCATC | 8 cut(s) | 45, 208, 313, 1280, 1713, 1743, 1770, 1842 |
| MaeI | CTAG | 8 cut(s) | 9, 35, 269, 800, 1340, 1616, 1757, 1853 |
| MaeII | ACGT | 3 cut(s) | 409, 749, 1098 |
| MaeIII | GTNAC | 2 cut(s) | 415, 795 |
| MalI | GATC | 6 cut(s) | 68, 162, 837, 1296, 1401, 1725 |
| MboI | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| MboII | GAAGA | 9 cut(s) | 64, 66, 504, 611, 993, 1067, 1271, 1558, 1664 |
| MfeI | CAATTG | 3 cut(s) | 62, 1686, 1866 |
| MhlI | GDGCHC | 3 cut(s) | 140, 1150, 1698 |
| MlsI | TGGCCA | 3 cut(s) | 486, 758, 1382 |
| MluNI | TGGCCA | 3 cut(s) | 486, 758, 1382 |
| Mly113I | GGCGCC | 1 cut(s) | 1846 |
| MlyI | GAGTC | 2 cut(s) | 23, 152 |
| MmeI | TCCRAC | 1 cut(s) | 1839 |
| Mox20I | TGGCCA | 3 cut(s) | 486, 758, 1382 |
| MroXI | GAANNNNTTC | 4 cut(s) | 717, 997, 1217, 1278 |
| MscI | TGGCCA | 3 cut(s) | 486, 758, 1382 |
| MseI | TTAA | 4 cut(s) | 860, 927, 987, 1032 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1101 |
| Msp20I | TGGCCA | 3 cut(s) | 486, 758, 1382 |
| MspA1I | CMGCKG | 1 cut(s) | 195 |
| MspI | CCGG | 1 cut(s) | 207 |
| MspR9I | CCNGG | 5 cut(s) | 170, 208, 779, 1184, 1468 |
| MunI | CAATTG | 3 cut(s) | 62, 1686, 1866 |
| Mva1269I | GAATGC | 2 cut(s) | 1013, 1521 |
| MvaI | CCWGG | 4 cut(s) | 170, 779, 1184, 1468 |
| MvnI | CGCG | 2 cut(s) | 195, 822 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 45, 349, 483, 702, 764 |
| NarI | GGCGCC | 1 cut(s) | 1846 |
| NciI | CCSGG | 1 cut(s) | 208 |
| NcoI | CCATGG | 2 cut(s) | 487, 1437 |
| NdeI | CATATG | 1 cut(s) | 916 |
| NdeII | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| NlaIII | CATG | 4 cut(s) | 167, 491, 1441, 1738 |
| NlaIV | GGNNCC | 6 cut(s) | 635, 697, 970, 1435, 1715, 1847 |
| PasI | CCCWGGG | 1 cut(s) | 778 |
| PctI | GAATGC | 2 cut(s) | 1013, 1521 |
| PdmI | GAANNNNTTC | 4 cut(s) | 717, 997, 1217, 1278 |
| PfeI | GAWTC | 4 cut(s) | 853, 1302, 1638, 1669 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 262 |
| PfoI | TCCNGGA | 2 cut(s) | 1182, 1466 |
| PkrI | GCNGC | 5 cut(s) | 60, 155, 194, 1521, 1524 |
| PleI | GAGTC | 2 cut(s) | 22, 151 |
| PluTI | GGCGCC | 1 cut(s) | 1849 |
| PpsI | GAGTC | 2 cut(s) | 22, 151 |
| Ppu21I | YACGTR | 1 cut(s) | 410 |
| PpuMI | RGGWCCY | 1 cut(s) | 1808 |
| PsiI | TTATAA | 1 cut(s) | 1608 |
| Psp124BI | GAGCTC | 1 cut(s) | 1150 |
| Psp5II | RGGWCCY | 1 cut(s) | 1808 |
| Psp6I | CCWGG | 4 cut(s) | 168, 777, 1182, 1466 |
| PspGI | CCWGG | 4 cut(s) | 168, 777, 1182, 1466 |
| PspN4I | GGNNCC | 6 cut(s) | 635, 697, 970, 1435, 1715, 1847 |
| PspPI | GGNCC | 3 cut(s) | 633, 1434, 1808 |
| PspPPI | RGGWCCY | 1 cut(s) | 1808 |
| PstI | CTGCAG | 1 cut(s) | 1807 |
| RsaI | GTAC | 5 cut(s) | 267, 408, 559, 953, 1029 |
| RsaNI | GTAC | 5 cut(s) | 266, 407, 558, 952, 1028 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1101 |
| SacI | GAGCTC | 1 cut(s) | 1150 |
| SacII | CCGCGG | 1 cut(s) | 196 |
| SaqAI | TTAA | 4 cut(s) | 860, 927, 987, 1032 |
| SatI | GCNGC | 5 cut(s) | 59, 154, 193, 1520, 1523 |
| Sau3AI | GATC | 6 cut(s) | 66, 160, 835, 1294, 1399, 1723 |
| Sau96I | GGNCC | 3 cut(s) | 633, 1434, 1808 |
| SchI | GAGTC | 2 cut(s) | 23, 152 |
| ScrFI | CCNGG | 5 cut(s) | 170, 208, 779, 1184, 1468 |
| SduI | GDGCHC | 3 cut(s) | 140, 1150, 1698 |
| SfaNI | GCATC | 8 cut(s) | 45, 208, 313, 1280, 1713, 1743, 1770, 1842 |
| SfcI | CTRYAG | 4 cut(s) | 548, 826, 1422, 1803 |
| SfoI | GGCGCC | 1 cut(s) | 1847 |
| Sfr303I | CCGCGG | 1 cut(s) | 196 |
| SgrBI | CCGCGG | 1 cut(s) | 196 |
| SinI | GGWCC | 3 cut(s) | 633, 1434, 1808 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1101 |
| SmlI | CTYRAG | 2 cut(s) | 896, 1526 |
| SmoI | CTYRAG | 2 cut(s) | 896, 1526 |
| SnaBI | TACGTA | 1 cut(s) | 410 |
| SpeI | ACTAGT | 1 cut(s) | 268 |
| SsiI | CCGC | 7 cut(s) | 153, 190, 193, 195, 234, 245, 1130 |
| SspDI | GGCGCC | 1 cut(s) | 1845 |
| SspI | AATATT | 2 cut(s) | 1333, 1552 |
| SspMI | CTAG | 8 cut(s) | 9, 35, 269, 800, 1340, 1616, 1757, 1853 |
| SstI | GAGCTC | 1 cut(s) | 1150 |
| StyD4I | CCNGG | 5 cut(s) | 168, 206, 777, 1182, 1466 |
| StyI | CCWWGG | 6 cut(s) | 478, 487, 964, 1222, 1395, 1437 |
| TaaI | ACNGT | 5 cut(s) | 373, 552, 763, 982, 1744 |
| TaiI | ACGT | 3 cut(s) | 412, 752, 1101 |
| TaqI | TCGA | 5 cut(s) | 50, 108, 856, 1314, 1647 |
| TatI | WGTACW | 2 cut(s) | 557, 951 |
| TauI | GCSGC | 2 cut(s) | 156, 195 |
| TfiI | GAWTC | 4 cut(s) | 853, 1302, 1638, 1669 |
| Tru1I | TTAA | 4 cut(s) | 860, 927, 987, 1032 |
| Tru9I | TTAA | 4 cut(s) | 860, 927, 987, 1032 |
| TscAI | CASTG | 2 cut(s) | 1715, 1725 |
| TseI | GCWGC | 3 cut(s) | 58, 1519, 1522 |
| TspDTI | ATGAA | 9 cut(s) | 94, 417, 774, 903, 1083, 1185, 1271, 1596, 1751 |
| TspGWI | ACGGA | 2 cut(s) | 416, 990 |
| TspRI | CASTG | 2 cut(s) | 1715, 1725 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 262 |
| VneI | GTGCAC | 1 cut(s) | 136 |
| VpaK11BI | GGWCC | 3 cut(s) | 633, 1434, 1808 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1616 |
| XapI | RAATTY | 4 cut(s) | 26, 275, 1471, 1584 |
| XbaI | TCTAGA | 1 cut(s) | 1615 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 594 |
| XmnI | GAANNNNTTC | 4 cut(s) | 717, 997, 1217, 1278 |
| XspI | CTAG | 8 cut(s) | 9, 35, 269, 800, 1340, 1616, 1757, 1853 |