Rroxscaffold_4G00320890

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000107
Physical Location & Seq
Forward (+)
49918984 .. 49937057
18074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320890.1

Sequence Viewer

Length: 2097 bp
ATGCAGCGACTAATCCTCATATCAGCCTATTGCATATTAATGTCAATGGCTTTCTTCAAGGTGTGCCATAATGCATGCACAGAATCAGAGTGTGGTGACCATGATGATCATCGCCTTGCTATTCATGTCCCGGTCAAGCGCGTCCGTTGTGGTCGTTTTCAGCAGCAGAGCGACAAGACTGTTGTTGAGCAGAAGCAAATGGTAGTAAAATTCTTTGTGAAACACATAGATTATAAGCACCAGAAAATTCAAGTACATATTCCAGATGATTGCTTACTCCTAAAGCAATTAGAAACCCCAAACATGAAGCCAATGTTCTTTCCTCTCAACTTCCCATCTGCAGGCTACGATGAGGATTGCATCGGCTTATTCAACTGTCCCACAGCTATTTATCCGCACAAACAACTGAAACAAGTCCCCTGCCTCAGTGACCTGGTATACAAAAATATCTATGCCGTTTCTTCATTCGCTGAAGCCCCACAGTACTGTACAAAGATGTATGATTTAATAAATCCATACGGACATGTGTATCCAGAGTATCTTTGGTGGTCTACACCAGATTGTGCAGAATGTGAAGCGAAGCGTAAGAAGTGTCAATTGAAGATCAACGGCACCAAAAGTGAAATTGAATGTGTTCATTTTAGCAAACCAAGTGCAACTGTGACTACAATCATTTTACTACTATCGGTGACTGTAGTCTATAGATCTCATAAAAAGGAAAAGGAGAATCAATTGAAAATTGAAACATTCCTAGAGGATTACAAAGCGCTCAAGCCAAGCAGATATTCCTACGCAGATATCAAAAGAATTACAAATCAATTTGAGGACAAGTTGGGCCAAGGAGCGTATGGAACTGTTTTCAAAGGGAAGCTTTCATCTGAAGTTTCTGTTGCTGTTAAAGTCCTCAACAATTCCGAAAACGGAGATGGGGAAGAATTCATCAATGAGGTGGGAACAATGGGTCATATCCACCATGTCAACGTGGTTCGCATGGTTGGTTTCTGTGCAGAAGGATTTAGACGAGCTCTTGTTTATGAGTTTTTCCCCAATGGTTCACTACAGGATTTCATTTCATCAGCAGACAATAAGAATTCTTTCCTTGGTTGGGATAAGTTAAAAGATATTTCTCTAGGCATTGCCAAAGGAATTGAATATCTACACCAAGGATGCGATCAACGAATTCTTCATTTTGATATCAAACCTCAAAATGTTTTGCTAGATCAAAACTTCACTCCAAAAATTTCTGATTTTGGGATGTCCAAGTTGTGTGCCAAGGATCAAAGTATAGTGTCAATGACTATGGCTAGGGGGACTATAGGCTATATTGCGCCTGAAGTGTTCTCCCGGAATTTTGGAAATGTGTCATATAAGTCAGATGTATATAGTTTTGGAATGTTGTTGCTCGAGATTGTAGGAGGGAGGAAGAATATTGGTGAAACCATGGATAACTCTGGGGAAGTATACTACCCAGAATGGATACATAATCTTTTAGATGAAGGGGAAGACCTTCGAGTCCATATTGGGGAAGAAGGATCGAGAATAGATTCTCGAATTCCAAAAGAACTAGCAATTGTAGGCCTTTGGTGTATCCAATGGCACCCAATGAATCGTCCATCCATGAAATCAGTGGTTCAGATGTTGGGAGGAAGAGAAAACTTAACCATGCCACCTAATCCTTTTGCCTCTACAGGTCCTACAAGAACAAATGCAACAAGTCGCCCGGCTGCAAGACGCTTCAACCTCGACTTAGAAGCAATTGTTGAATTAGAGGGCCTCCCACGCATCCTAGGCGGGGCCATGGCCTATAACGCTAGAGTGCCACTCGCTTTGGCGTTGGCACCATGCCTACCTCCGTGTAGGTTGACGAGCGTTTGGAATATTGTTATATTTGACTTTTTAGAAGACGCAGTTGGATTAGAGAAAAGACTGCTTCAAAGCCGTGACGGAGGCGCGGGGCTTGGTGGACTGAGGCCGGAGGCATCCCGGATGGCTTCGGGCAGCGATCGAGGTCGGAGGAGGTCGGGGTTGCAGGTTTCGGGCAGCGACCTCACCTACAACTTCCGGTTCTGGGCAGTGCTGCAAATCGGTCGCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

698

Amino Acids

78.74

Weight (kDa)

8.35

Isoelectric Point (pI)

48.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 273 - 547 1.2e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 274 - 545 1.4e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 234
AasI GACNNNNNNGTC 1 cut(s) 1511
Acc36I ACCTGC 1 cut(s) 2020
AccB1I GGYRCC 3 cut(s) 611, 1596, 1837
AccI GTMKAC 3 cut(s) 438, 551, 1461
AccII CGCG 2 cut(s) 141, 1952
AciI CCGC 3 cut(s) 395, 1791, 1952
AclWI GGATC 2 cut(s) 1284, 1540
AcsI RAATTY 8 cut(s) 209, 246, 935, 1090, 1179, 1239, 1348, 1551
AcuI CTGAAG 3 cut(s) 492, 900, 1353
AfaI GTAC 3 cut(s) 255, 485, 490
AfeI AGCGCT 1 cut(s) 768
AfiI CCNNNNNNNGG 5 cut(s) 341, 1105, 1522, 1792, 2068
AflIII ACRYGT 1 cut(s) 523
AjnI CCWGG 1 cut(s) 432
AjuI GAANNNNNNNTTGG 4 cut(s) 769, 801, 1893, 1925
AluBI AGCT 3 cut(s) 386, 871, 1025
AluI AGCT 3 cut(s) 386, 871, 1025
Alw21I GWGCWC 1 cut(s) 1027
AlwI GGATC 2 cut(s) 1284, 1540
Ama87I CYCGRG 1 cut(s) 1403
Aor51HI AGCGCT 1 cut(s) 768
AoxI GGCC 6 cut(s) 835, 1576, 1771, 1794, 1800, 1970
ApeKI GCWGC 6 cut(s) 4, 163, 1724, 1998, 2040, 2077
ApoI RAATTY 8 cut(s) 209, 246, 935, 1090, 1179, 1239, 1348, 1551
AseI ATTAAT 1 cut(s) 38
Asp700I GAANNNNTTC 4 cut(s) 633, 1094, 1506, 1543
AspA2I CCTAGG 1 cut(s) 1786
AspLEI GCGC 4 cut(s) 141, 769, 1330, 1952
AspS9I GGNCC 4 cut(s) 835, 1691, 1771, 1794
AsuC2I CCSGG 4 cut(s) 131, 1345, 1721, 1984
AsuHPI GGTGA 4 cut(s) 107, 700, 1445, 2041
AvaI CYCGRG 1 cut(s) 1403
AvaII GGWCC 1 cut(s) 1691
AvrII CCTAGG 1 cut(s) 1786
BaeI ACNNNNGTAYC 2 cut(s) 512, 545
BanI GGYRCC 3 cut(s) 611, 1596, 1837
BanII GRGCYC 1 cut(s) 1027
BbsI GAAGAC 2 cut(s) 1509, 1908
Bbv12I GWGCWC 1 cut(s) 1027
BbvI GCAGC 6 cut(s) 16, 175, 1711, 2010, 2052, 2064
BccI CCATC 4 cut(s) 343, 920, 1621, 1981
BceAI ACGGC 3 cut(s) 440, 625, 1923
BcgI CGANNNNNNTGC 1 cut(s) 2069
BciT130I CCWGG 1 cut(s) 434
BciVI GTATCC 3 cut(s) 540, 1470, 1598
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 4 cut(s) 131, 1345, 1721, 1984
BfaI CTAG 7 cut(s) 752, 1130, 1217, 1305, 1565, 1787, 1812
BfmI CTRYAG 6 cut(s) 339, 693, 700, 1058, 1314, 1686
BfoI RGCGCY 1 cut(s) 770
BfuAI ACCTGC 1 cut(s) 2020
BfuI GTATCC 3 cut(s) 540, 1470, 1598
BglII AGATCT 1 cut(s) 704
BisI GCNGC 6 cut(s) 5, 164, 1725, 1999, 2041, 2078
BlnI CCTAGG 1 cut(s) 1786
BlsI GCNGC 6 cut(s) 6, 165, 1726, 2000, 2042, 2079
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 5 cut(s) 131, 434, 1345, 1721, 1984
Bme18I GGWCC 1 cut(s) 1691
BmeT110I CYCGRG 1 cut(s) 1403
BmgT120I GGNCC 4 cut(s) 835, 1691, 1771, 1794
BmiI GGNNCC 4 cut(s) 613, 1598, 1795, 1839
BmrFI CCNGG 5 cut(s) 131, 434, 1345, 1721, 1984
BmsI GCATC 4 cut(s) 369, 1157, 1791, 1988
BoxI GACNNNNGTC 1 cut(s) 695
BpiI GAAGAC 2 cut(s) 1509, 1908
BplI GAGNNNNNCTC 2 cut(s) 1806, 1838
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 4 cut(s) 131, 1345, 1721, 1984
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
BsaWI WCCGGW 1 cut(s) 2061
Bsc4I CCNNNNNNNGG 5 cut(s) 341, 1105, 1522, 1792, 2068
Bse118I RCCGGY 1 cut(s) 2091
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
BseGI GGATG 6 cut(s) 1172, 1260, 1613, 1782, 1979, 1992
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 5 cut(s) 341, 1105, 1522, 1792, 2068
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 2 cut(s) 439, 1958
BseRI GAGGAG 1 cut(s) 2029
BseXI GCAGC 6 cut(s) 16, 175, 1711, 2010, 2052, 2064
BsgI GTGCAG 2 cut(s) 585, 1026
Bsh1236I CGCG 2 cut(s) 141, 1952
Bsh1285I CGRYCG 2 cut(s) 2005, 2089
BshFI GGCC 6 cut(s) 837, 1578, 1773, 1796, 1802, 1972
BshNI GGYRCC 3 cut(s) 611, 1596, 1837
BsiEI CGRYCG 2 cut(s) 2005, 2089
BsiHKAI GWGCWC 1 cut(s) 1027
BsiHKCI CYCGRG 1 cut(s) 1403
BsiSI CCGG 7 cut(s) 131, 1345, 1721, 1973, 1984, 2062, 2092
BslFI GGGAC 4 cut(s) 113, 363, 401, 1324
BslI CCNNNNNNNGG 5 cut(s) 341, 1105, 1522, 1792, 2068
BsmFI GGGAC 4 cut(s) 113, 363, 401, 1324
BsnI GGCC 6 cut(s) 837, 1578, 1773, 1796, 1802, 1972
BsoBI CYCGRG 1 cut(s) 1403
Bsp1286I GDGCHC 1 cut(s) 1027
Bsp1407I TGTACA 1 cut(s) 488
Bsp143I GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
Bsp19I CCATGG 2 cut(s) 1440, 1797
BspACI CCGC 3 cut(s) 395, 1791, 1952
BspANI GGCC 6 cut(s) 837, 1578, 1773, 1796, 1802, 1972
BspCNI CTCAG 2 cut(s) 438, 1959
BspFNI CGCG 2 cut(s) 141, 1952
BspLI GGNNCC 4 cut(s) 613, 1598, 1795, 1839
BspMAI CTGCAG 1 cut(s) 343
BspMI ACCTGC 1 cut(s) 2020
BspPI GGATC 2 cut(s) 1284, 1540
BspT107I GGYRCC 3 cut(s) 611, 1596, 1837
BsrDI GCAATG 1 cut(s) 1134
BsrFI RCCGGY 1 cut(s) 2091
BsrGI TGTACA 1 cut(s) 488
BssAI RCCGGY 1 cut(s) 2091
BssECI CCNNGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
BssMI GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
BssNAI GTATAC 2 cut(s) 439, 1462
BssT1I CCWWGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
Bst1107I GTATAC 2 cut(s) 439, 1462
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 7 cut(s) 181, 377, 483, 488, 661, 694, 856
Bst6I CTCTTC 1 cut(s) 1642
BstAUI TGTACA 1 cut(s) 488
BstC8I GCNNGC 2 cut(s) 76, 343
BstDEI CTNAG 3 cut(s) 425, 1747, 1967
BstDSI CCRYGG 2 cut(s) 1440, 1797
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 6 cut(s) 1172, 1260, 1613, 1782, 1979, 1992
BstFNI CGCG 2 cut(s) 141, 1952
BstH2I RGCGCY 1 cut(s) 770
BstHHI GCGC 4 cut(s) 141, 769, 1330, 1952
BstKTI GATC 8 cut(s) 109, 606, 707, 1174, 1222, 1279, 1535, 2005
BstMBI GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
BstMCI CGRYCG 2 cut(s) 2005, 2089
BstMWI GCNNNNNNNGC 3 cut(s) 1779, 1788, 1808
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 2 cut(s) 78, 527
BstPAI GACNNNNGTC 1 cut(s) 695
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 5 cut(s) 129, 432, 1343, 1719, 1982
BstSFI CTRYAG 6 cut(s) 339, 693, 700, 1058, 1314, 1686
BstUI CGCG 2 cut(s) 141, 1952
BstV1I GCAGC 6 cut(s) 16, 175, 1711, 2010, 2052, 2064
BstV2I GAAGAC 2 cut(s) 1509, 1908
BstX2I RGATCY 1 cut(s) 704
BstYI RGATCY 1 cut(s) 704
BstZ17I GTATAC 2 cut(s) 439, 1462
BsuI GTATCC 3 cut(s) 540, 1470, 1598
BsuRI GGCC 6 cut(s) 837, 1578, 1773, 1796, 1802, 1972
BtgI CCRYGG 2 cut(s) 1440, 1797
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 6 cut(s) 1172, 1260, 1613, 1782, 1979, 1992
BtsI GCAGTG 1 cut(s) 2079
BtsIMutI CAGTG 3 cut(s) 433, 1632, 2079
BveI ACCTGC 1 cut(s) 2020
Cac8I GCNNGC 2 cut(s) 76, 343
CfoI GCGC 4 cut(s) 141, 769, 1330, 1952
Cfr10I RCCGGY 1 cut(s) 2091
Cfr13I GGNCC 4 cut(s) 835, 1691, 1771, 1794
CseI GACGC 3 cut(s) 130, 1740, 1913
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 3 cut(s) 254, 484, 489
CspCI CAANNNNNGTGG 2 cut(s) 1809, 1844
CviQI GTAC 3 cut(s) 254, 484, 489
DdeI CTNAG 3 cut(s) 425, 1747, 1967
DpnI GATC 8 cut(s) 108, 605, 706, 1173, 1221, 1278, 1534, 2004
DpnII GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
DrdI GACNNNNNNGTC 1 cut(s) 1511
DseDI GACNNNNNNGTC 1 cut(s) 1511
Eam1104I CTCTTC 1 cut(s) 1642
EarI CTCTTC 1 cut(s) 1642
Ecl136II GAGCTC 1 cut(s) 1025
Eco130I CCWWGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
Eco147I AGGCCT 1 cut(s) 1578
Eco24I GRGCYC 1 cut(s) 1027
Eco32I GATATC 2 cut(s) 799, 1195
Eco47I GGWCC 1 cut(s) 1691
Eco47III AGCGCT 1 cut(s) 768
Eco53kI GAGCTC 1 cut(s) 1025
Eco57I CTGAAG 3 cut(s) 492, 900, 1353
Eco88I CYCGRG 1 cut(s) 1403
Eco91I GGTNACC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 1025
EcoO109I RGGNCCY 2 cut(s) 1691, 1771
EcoO65I GGTNACC 1 cut(s) 95
EcoRI GAATTC 4 cut(s) 935, 1090, 1179, 1551
EcoRII CCWGG 1 cut(s) 432
EcoRV GATATC 2 cut(s) 799, 1195
EcoT14I CCWWGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
EcoT22I ATGCAT 1 cut(s) 76
EcoT38I GRGCYC 1 cut(s) 1027
ErhI CCWWGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
FalI AAGNNNNNCTT 2 cut(s) 855, 887
FaqI GGGAC 4 cut(s) 113, 363, 401, 1324
FauI CCCGC 2 cut(s) 1784, 1945
FbaI TGATCA 1 cut(s) 106
FblI GTMKAC 3 cut(s) 438, 551, 1461
Fnu4HI GCNGC 6 cut(s) 5, 164, 1725, 1999, 2041, 2078
FokI GGATG 6 cut(s) 1179, 1267, 1600, 1769, 1966, 1999
FriOI GRGCYC 1 cut(s) 1027
Fsp4HI GCNGC 6 cut(s) 5, 164, 1725, 1999, 2041, 2078
FspBI CTAG 7 cut(s) 752, 1130, 1217, 1305, 1565, 1787, 1812
GlaI GCGC 4 cut(s) 140, 768, 1329, 1951
GluI GCNGC 6 cut(s) 5, 164, 1725, 1999, 2041, 2078
HaeII RGCGCY 1 cut(s) 770
HaeIII GGCC 6 cut(s) 837, 1578, 1773, 1796, 1802, 1972
HapII CCGG 7 cut(s) 131, 1345, 1721, 1973, 1984, 2062, 2092
HgaI GACGC 3 cut(s) 130, 1740, 1913
HhaI GCGC 4 cut(s) 141, 769, 1330, 1952
Hin6I GCGC 4 cut(s) 139, 767, 1328, 1950
HinP1I GCGC 4 cut(s) 139, 767, 1328, 1950
HincII GTYRAC 2 cut(s) 979, 1863
HindII GTYRAC 2 cut(s) 979, 1863
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 5 cut(s) 83, 727, 1512, 1544, 1606
HpaII CCGG 7 cut(s) 131, 1345, 1721, 1973, 1984, 2062, 2092
HphI GGTGA 4 cut(s) 107, 700, 1445, 2041
Hpy166II GTNNAC 7 cut(s) 439, 552, 979, 1055, 1462, 1863, 1964
Hpy188I TCNGA 7 cut(s) 88, 880, 916, 1246, 1375, 1635, 2013
Hpy188III TCNNGA 5 cut(s) 263, 533, 1405, 1536, 1548
Hpy8I GTNNAC 7 cut(s) 439, 552, 979, 1055, 1462, 1863, 1964
HpyAV CCTTC 4 cut(s) 1004, 1490, 1517, 1523
HpyCH4III ACNGT 7 cut(s) 181, 377, 483, 488, 661, 694, 856
HpyCH4IV ACGT 1 cut(s) 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 1779, 1788, 1808
HpyF3I CTNAG 3 cut(s) 425, 1747, 1967
HpySE526I ACGT 1 cut(s) 981
HspAI GCGC 4 cut(s) 139, 767, 1328, 1950
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
LmnI GCTCC 1 cut(s) 842
Lsp1109I GCAGC 6 cut(s) 16, 175, 1711, 2010, 2052, 2064
LweI GCATC 4 cut(s) 369, 1157, 1791, 1988
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 7 cut(s) 752, 1130, 1217, 1305, 1565, 1787, 1812
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 5 cut(s) 95, 428, 661, 688, 1940
MalI GATC 8 cut(s) 108, 605, 706, 1173, 1221, 1278, 1534, 2004
MboI GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
MfeI CAATTG 4 cut(s) 596, 731, 1569, 1755
MflI RGATCY 1 cut(s) 704
MhlI GDGCHC 1 cut(s) 1027
MlyI GAGTC 1 cut(s) 1521
MmeI TCCRAC 2 cut(s) 1891, 1991
Mph1103I ATGCAT 1 cut(s) 76
MroXI GAANNNNTTC 4 cut(s) 633, 1094, 1506, 1543
MseI TTAA 5 cut(s) 38, 506, 897, 1115, 1658
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 7 cut(s) 131, 1345, 1721, 1973, 1984, 2062, 2092
MspR9I CCNGG 5 cut(s) 131, 434, 1345, 1721, 1984
MunI CAATTG 4 cut(s) 596, 731, 1569, 1755
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 2 cut(s) 141, 1952
MwoI GCNNNNNNNGC 3 cut(s) 1779, 1788, 1808
NciI CCSGG 4 cut(s) 131, 1345, 1721, 1984
NcoI CCATGG 2 cut(s) 1440, 1797
NdeII GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
NlaIV GGNNCC 4 cut(s) 613, 1598, 1795, 1839
NmuCI GTSAC 5 cut(s) 95, 428, 661, 688, 1940
NsiI ATGCAT 1 cut(s) 76
NspI RCATGY 2 cut(s) 78, 527
PaeI GCATGC 1 cut(s) 78
PaeR7I CTCGAG 1 cut(s) 1403
PceI AGGCCT 1 cut(s) 1578
PciI ACATGT 1 cut(s) 523
PdmI GAANNNNTTC 4 cut(s) 633, 1094, 1506, 1543
PfeI GAWTC 4 cut(s) 83, 727, 1544, 1606
PfoI TCCNGGA 2 cut(s) 1343, 1982
PkrI GCNGC 6 cut(s) 6, 165, 1726, 2000, 2042, 2079
Ple19I CGATCG 1 cut(s) 2005
PleI GAGTC 1 cut(s) 1520
PpsI GAGTC 1 cut(s) 1520
PpuMI RGGWCCY 1 cut(s) 1691
PscI ACATGT 1 cut(s) 523
PshAI GACNNNNGTC 1 cut(s) 695
PshBI ATTAAT 1 cut(s) 38
PsiI TTATAA 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 1027
Psp5II RGGWCCY 1 cut(s) 1691
Psp6I CCWGG 1 cut(s) 432
PspEI GGTNACC 1 cut(s) 95
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 4 cut(s) 613, 1598, 1795, 1839
PspPI GGNCC 4 cut(s) 835, 1691, 1771, 1794
PspPPI RGGWCCY 1 cut(s) 1691
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 1 cut(s) 704
PvuI CGATCG 1 cut(s) 2005
RsaI GTAC 3 cut(s) 255, 485, 490
RsaNI GTAC 3 cut(s) 254, 484, 489
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 1027
SaqAI TTAA 5 cut(s) 38, 506, 897, 1115, 1658
SatI GCNGC 6 cut(s) 5, 164, 1725, 1999, 2041, 2078
Sau3AI GATC 8 cut(s) 106, 603, 704, 1171, 1219, 1276, 1532, 2002
Sau96I GGNCC 4 cut(s) 835, 1691, 1771, 1794
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 1 cut(s) 1521
ScrFI CCNGG 5 cut(s) 131, 434, 1345, 1721, 1984
SduI GDGCHC 1 cut(s) 1027
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 4 cut(s) 369, 1157, 1791, 1988
SfcI CTRYAG 6 cut(s) 339, 693, 700, 1058, 1314, 1686
Sfr274I CTCGAG 1 cut(s) 1403
SgrAI CRCCGGYG 1 cut(s) 2091
SinI GGWCC 1 cut(s) 1691
SlaI CTCGAG 1 cut(s) 1403
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 770, 1403
SmoI CTYRAG 2 cut(s) 770, 1403
SphI GCATGC 1 cut(s) 78
SseBI AGGCCT 1 cut(s) 1578
SsiI CCGC 3 cut(s) 395, 1791, 1952
SspI AATATT 2 cut(s) 1429, 1879
SspMI CTAG 7 cut(s) 752, 1130, 1217, 1305, 1565, 1787, 1812
SstI GAGCTC 1 cut(s) 1027
StuI AGGCCT 1 cut(s) 1578
StyD4I CCNGG 5 cut(s) 129, 432, 1343, 1719, 1982
StyI CCWWGG 7 cut(s) 838, 1099, 1162, 1272, 1440, 1786, 1797
TaaI ACNGT 7 cut(s) 181, 377, 483, 488, 661, 694, 856
TaiI ACGT 1 cut(s) 984
TaqI TCGA 6 cut(s) 1404, 1510, 1535, 1549, 1743, 2005
TaqII GACCGA 1 cut(s) 2075
TatI WGTACW 3 cut(s) 253, 483, 488
TfiI GAWTC 4 cut(s) 83, 727, 1544, 1606
Tru1I TTAA 5 cut(s) 38, 506, 897, 1115, 1658
Tru9I TTAA 5 cut(s) 38, 506, 897, 1115, 1658
TscAI CASTG 3 cut(s) 433, 1632, 2079
TseFI GTSAC 5 cut(s) 95, 428, 661, 688, 1940
TseI GCWGC 6 cut(s) 4, 163, 1724, 1998, 2040, 2077
Tsp45I GTSAC 5 cut(s) 95, 428, 661, 688, 1940
TspGWI ACGGA 5 cut(s) 134, 534, 936, 1842, 1959
TspRI CASTG 3 cut(s) 433, 1632, 2079
VpaK11BI GGWCC 1 cut(s) 1691
VspI ATTAAT 1 cut(s) 38
XapI RAATTY 8 cut(s) 209, 246, 935, 1090, 1179, 1239, 1348, 1551
XceI RCATGY 2 cut(s) 78, 527
XcmI CCANNNNNNNNNTGG 3 cut(s) 540, 845, 1624
XhoI CTCGAG 1 cut(s) 1403
XmaJI CCTAGG 1 cut(s) 1786
XmiI GTMKAC 3 cut(s) 438, 551, 1461
XmnI GAANNNNTTC 4 cut(s) 633, 1094, 1506, 1543
XspI CTAG 7 cut(s) 752, 1130, 1217, 1305, 1565, 1787, 1812
ZrmI AGTACT 1 cut(s) 485
Zsp2I ATGCAT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.