FvH4_4g34420

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000107
Physical Location & Seq
Forward (+)
32216774 .. 32220519
3746 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34420.t3

Sequence Viewer

Length: 1296 bp
ATGCGTACTCTTTCATCAGTACCATACTGGTCTGAGCTTTATTTGGAATGGTCTGAACCAAATTGTACACTATGTGAAGCACAGGGAAATATCTGTGGATTGAAGAACAATGGCACCACTAGTACTGAGATTGAATGCCTTCCCATGAAGAAAGGAGGTTCGGGGAAAACTTTATTAGCTACGGGTACATCCCTGGGTTCATTTGTACTCGTACTACTTGCTGGTGCAGCTTTTCATGCTTATAGTTCTGATAGAAAGGAGAAACAGAATCAATTAAAAATTGAAAGATTTTTAGAGGATTACAGAGCTCTCAAACCAAGCAGATACTCTTATTCAGACATCAAGAGGATTACAGATCAGTTCAAGGACAAATTAGGCCAAGGGGCATATGGGACCGTTTATAAGGGAGAGCTTTCTTCTGAATGTTTTGTTGCCGTGAAAGTCCTCACTAGTACTAAAGGAGATGGGGAAGAGTTTGTTAATGAAGTGGGAACAATGGGTCATATCCATCATGTCAATGTGGTTCGCTTGGTTGGATTCTGCGCTGATGGGTTTAGAAGAGCTCTTGTTTATGACTTCTTACCTAATGGTTCACTGCAAGATTTCATTTCATCAGCAGACAATAACAATTCTTTCCTTGGTTGGGATAAATTGCAAGATATTGCTCTAGGCATAGCCAAAGGAATTGAATATCTGCACCAGGGGTGCGATCAACGAATCCTCCATTTTGATATCAAACCCCACAATGTTCTGCTTGACCATAACTTCACCCCAAAGATTTCTGATTTTGGTTTGGCCAAGTTATGTTCCAAGGATCAGAGTATAGTGTCAATGACTACAGCTAGGGGAACAATGGGGTACATTGCACCTGAAGTGTTCTCCAGGAACTTCGGGAATGTGTCCTATAAGTCAGATGTGTATAGTTATGGAATGGTACTGCTCGAGATGGTAGGAGGAAGAAAGAACATCAGTTCAACCACAGAGAACGCACCTGAAGTTTACTACCCAGAATGGATCTATAATCTACTAGAAGAAGGTGAAGACCTCCGAATCCATGTTGGGGAAGAAGCAGATGCTAAAATTGCAAAGAGACTTGCGATTGTGGGTCTCTGGTGCATCCAATGGCACCCGGTTGATCGTCCCTCTATGAAAGGGGTGGTTCAGATGTTGGAAGGAGGAGAAAACTTGACTATGCCTCCAAATCCTTTTGCCTCTCAAGGTCCTGCAGGAACAAGTGCAAGCACACCTGCCAGAAGATTAAACCTTCAACTGGAACCAATTGCTGAGTTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

432

Amino Acids

47.74

Weight (kDa)

5.77

Isoelectric Point (pI)

36.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 80 - 349 7e-45 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 81 - 347 2.4e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AarI CACCTGC 1 cut(s) 1255
Acc36I ACCTGC 1 cut(s) 1255
AccB1I GGYRCC 2 cut(s) 113, 1125
AclWI GGATC 2 cut(s) 822, 1022
AcoI YGGCCR 1 cut(s) 795
AcuI CTGAAG 2 cut(s) 891, 1014
AdeI CACNNNGTG 1 cut(s) 74
AfiI CCNNNNNNNGG 3 cut(s) 643, 1060, 1270
AgsI TTSAA 7 cut(s) 103, 134, 284, 364, 689, 975, 1268
AhlI ACTAGT 2 cut(s) 119, 449
AjnI CCWGG 3 cut(s) 192, 699, 881
AluBI AGCT 7 cut(s) 37, 179, 230, 308, 412, 563, 842
AluI AGCT 7 cut(s) 37, 179, 230, 308, 412, 563, 842
Alw21I GWGCWC 2 cut(s) 310, 565
Alw26I GTCTC 2 cut(s) 1084, 1112
AlwI GGATC 2 cut(s) 822, 1022
Ama87I CYCGRG 1 cut(s) 941
AoxI GGCC 2 cut(s) 376, 795
ApeKI GCWGC 1 cut(s) 227
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 545
AspS9I GGNCC 2 cut(s) 393, 1220
AsuC2I CCSGG 1 cut(s) 1130
AsuHPI GGTGA 2 cut(s) 760, 1049
AvaI CYCGRG 1 cut(s) 941
AvaII GGWCC 2 cut(s) 393, 1220
BalI TGGCCA 1 cut(s) 797
BanI GGYRCC 2 cut(s) 113, 1125
BanII GRGCYC 2 cut(s) 310, 565
BbsI GAAGAC 1 cut(s) 1047
Bbv12I GWGCWC 2 cut(s) 310, 565
BbvI GCAGC 1 cut(s) 239
BccI CCATC 4 cut(s) 458, 516, 542, 940
BceAI ACGGC 1 cut(s) 419
BciT130I CCWGG 3 cut(s) 194, 701, 883
BcnI CCSGG 1 cut(s) 1130
BcoDI GTCTC 2 cut(s) 1084, 1112
BcuI ACTAGT 2 cut(s) 119, 449
BfaI CTAG 5 cut(s) 120, 450, 668, 843, 1028
BfmI CTRYAG 2 cut(s) 837, 1224
BfuAI ACCTGC 1 cut(s) 1255
BisI GCNGC 1 cut(s) 228
BlsI GCNGC 1 cut(s) 229
BmcAI AGTACT 2 cut(s) 124, 454
Bme1390I CCNGG 4 cut(s) 194, 701, 883, 1130
Bme18I GGWCC 2 cut(s) 393, 1220
BmeT110I CYCGRG 1 cut(s) 941
BmgT120I GGNCC 2 cut(s) 393, 1220
BmiI GGNNCC 4 cut(s) 115, 394, 1127, 1275
BmrFI CCNGG 4 cut(s) 194, 701, 883, 1130
BmsI GCATC 2 cut(s) 1063, 1125
BpiI GAAGAC 1 cut(s) 1047
BpmI CTGGAG 1 cut(s) 865
BpuEI CTTGAG 1 cut(s) 1200
BpuMI CCSGG 1 cut(s) 1130
BsaI GGTCTC 1 cut(s) 1112
BsaJI CCNNGG 6 cut(s) 192, 193, 379, 637, 700, 810
BsaXI ACNNNNNCTCC 4 cut(s) 705, 735, 1180, 1210
Bsc4I CCNNNNNNNGG 3 cut(s) 643, 1060, 1270
Bse1I ACTGG 2 cut(s) 32, 1275
Bse3DI GCAATG 1 cut(s) 861
BseBI CCWGG 3 cut(s) 194, 701, 883
BseDI CCNNGG 6 cut(s) 192, 193, 379, 637, 700, 810
BseGI GGATG 2 cut(s) 188, 1116
BseLI CCNNNNNNNGG 3 cut(s) 643, 1060, 1270
BseMI GCAATG 1 cut(s) 861
BseMII CTCAG 3 cut(s) 24, 117, 1275
BseNI ACTGG 2 cut(s) 32, 1275
BseRI GAGGAG 1 cut(s) 1191
BseXI GCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 246, 680
BshFI GGCC 2 cut(s) 378, 797
BshNI GGYRCC 2 cut(s) 113, 1125
BsiHKAI GWGCWC 2 cut(s) 310, 565
BsiHKCI CYCGRG 1 cut(s) 941
BsiSI CCGG 1 cut(s) 1130
BslFI GGGAC 2 cut(s) 406, 1125
BslI CCNNNNNNNGG 3 cut(s) 643, 1060, 1270
BsmAI GTCTC 2 cut(s) 1084, 1112
BsmFI GGGAC 2 cut(s) 406, 1125
BsmI GAATGC 1 cut(s) 140
BsnI GGCC 2 cut(s) 378, 797
Bso31I GGTCTC 1 cut(s) 1112
BsoBI CYCGRG 1 cut(s) 941
Bsp1286I GDGCHC 2 cut(s) 310, 565
Bsp1407I TGTACA 1 cut(s) 65
Bsp143I GATC 5 cut(s) 355, 709, 814, 1014, 1135
BspANI GGCC 2 cut(s) 378, 797
BspCNI CTCAG 3 cut(s) 25, 118, 1276
BspLI GGNNCC 4 cut(s) 115, 394, 1127, 1275
BspMAI CTGCAG 1 cut(s) 1228
BspMI ACCTGC 1 cut(s) 1255
BspPI GGATC 2 cut(s) 822, 1022
BspQI GCTCTTC 1 cut(s) 553
BspT107I GGYRCC 2 cut(s) 113, 1125
BspTNI GGTCTC 1 cut(s) 1112
BsrDI GCAATG 1 cut(s) 861
BsrGI TGTACA 1 cut(s) 65
BsrI ACTGG 2 cut(s) 32, 1275
BssECI CCNNGG 6 cut(s) 192, 193, 379, 637, 700, 810
BssMI GATC 5 cut(s) 355, 709, 814, 1014, 1135
BssT1I CCWWGG 3 cut(s) 379, 637, 810
Bst2UI CCWGG 3 cut(s) 194, 701, 883
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 2 cut(s) 465, 553
BstAUI TGTACA 1 cut(s) 65
BstC8I GCNNGC 1 cut(s) 1240
BstDEI CTNAG 3 cut(s) 33, 126, 1284
BstF5I GGATG 2 cut(s) 188, 1116
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 5 cut(s) 358, 712, 817, 1017, 1138
BstMAI GTCTC 2 cut(s) 1084, 1112
BstMBI GATC 5 cut(s) 355, 709, 814, 1014, 1135
BstMWI GCNNNNNNNGC 3 cut(s) 227, 236, 1082
BstNI CCWGG 3 cut(s) 194, 701, 883
BstSCI CCNGG 4 cut(s) 192, 699, 881, 1128
BstSFI CTRYAG 2 cut(s) 837, 1224
BstV1I GCAGC 1 cut(s) 239
BstV2I GAAGAC 1 cut(s) 1047
BstX2I RGATCY 1 cut(s) 1014
BstYI RGATCY 1 cut(s) 1014
BsuRI GGCC 2 cut(s) 378, 797
BtsCI GGATG 2 cut(s) 188, 1116
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 1 cut(s) 593
BveI ACCTGC 1 cut(s) 1255
Cac8I GCNNGC 1 cut(s) 1240
CfoI GCGC 1 cut(s) 545
Cfr13I GGNCC 2 cut(s) 393, 1220
CviAII CATG 4 cut(s) 145, 236, 512, 1055
DdeI CTNAG 3 cut(s) 33, 126, 1284
DpnI GATC 5 cut(s) 357, 711, 816, 1016, 1137
DpnII GATC 5 cut(s) 355, 709, 814, 1014, 1135
DraIII CACNNNGTG 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 795
Eam1104I CTCTTC 2 cut(s) 465, 553
EarI CTCTTC 2 cut(s) 465, 553
Ecl136II GAGCTC 2 cut(s) 308, 563
Eco130I CCWWGG 3 cut(s) 379, 637, 810
Eco24I GRGCYC 2 cut(s) 310, 565
Eco31I GGTCTC 1 cut(s) 1112
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 393, 1220
Eco53kI GAGCTC 2 cut(s) 308, 563
Eco57I CTGAAG 2 cut(s) 891, 1014
Eco88I CYCGRG 1 cut(s) 941
EcoICRI GAGCTC 2 cut(s) 308, 563
EcoO109I RGGNCCY 1 cut(s) 1220
EcoRII CCWGG 3 cut(s) 192, 699, 881
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 3 cut(s) 379, 637, 810
EcoT38I GRGCYC 2 cut(s) 310, 565
ErhI CCWWGG 3 cut(s) 379, 637, 810
FaeI CATG 4 cut(s) 148, 239, 515, 1058
FaqI GGGAC 2 cut(s) 406, 1125
FatI CATG 4 cut(s) 144, 235, 511, 1054
FauNDI CATATG 1 cut(s) 388
Fnu4HI GCNGC 1 cut(s) 228
FokI GGATG 2 cut(s) 175, 1103
FriOI GRGCYC 2 cut(s) 310, 565
Fsp4HI GCNGC 1 cut(s) 228
FspBI CTAG 5 cut(s) 120, 450, 668, 843, 1028
GlaI GCGC 1 cut(s) 544
GluI GCNGC 1 cut(s) 228
GsuI CTGGAG 1 cut(s) 865
HaeIII GGCC 2 cut(s) 378, 797
HapII CCGG 1 cut(s) 1130
HhaI GCGC 1 cut(s) 545
Hin1II CATG 4 cut(s) 148, 239, 515, 1058
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HinfI GANTC 4 cut(s) 268, 537, 717, 1050
HpaII CCGG 1 cut(s) 1130
HphI GGTGA 2 cut(s) 760, 1049
Hpy166II GTNNAC 3 cut(s) 68, 593, 1000
Hpy188III TCNNGA 3 cut(s) 343, 892, 943
Hpy8I GTNNAC 3 cut(s) 68, 593, 1000
HpyAV CCTTC 4 cut(s) 149, 1028, 1166, 1274
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4V TGCA 9 cut(s) 227, 598, 655, 697, 866, 1085, 1116, 1226, 1238
HpyF10VI GCNNNNNNNGC 3 cut(s) 227, 236, 1082
HpyF3I CTNAG 3 cut(s) 33, 126, 1284
Hsp92II CATG 4 cut(s) 148, 239, 515, 1058
HspAI GCGC 1 cut(s) 543
Kzo9I GATC 5 cut(s) 355, 709, 814, 1014, 1135
LguI GCTCTTC 1 cut(s) 553
Lsp1109I GCAGC 1 cut(s) 239
LweI GCATC 2 cut(s) 1063, 1125
MaeI CTAG 5 cut(s) 120, 450, 668, 843, 1028
MalI GATC 5 cut(s) 357, 711, 816, 1016, 1137
MboI GATC 5 cut(s) 355, 709, 814, 1014, 1135
MfeI CAATTG 1 cut(s) 1278
MflI RGATCY 1 cut(s) 1014
MhlI GDGCHC 2 cut(s) 310, 565
MlsI TGGCCA 1 cut(s) 797
MluCI AATT 9 cut(s) 61, 272, 279, 371, 628, 650, 684, 1080, 1278
MluNI TGGCCA 1 cut(s) 797
MmeI TCCRAC 2 cut(s) 514, 1149
Mox20I TGGCCA 1 cut(s) 797
MroXI GAANNNNTTC 1 cut(s) 138
MscI TGGCCA 1 cut(s) 797
MseI TTAA 3 cut(s) 275, 480, 1259
MslI CAYNNNNRTG 1 cut(s) 516
Msp20I TGGCCA 1 cut(s) 797
MspI CCGG 1 cut(s) 1130
MspR9I CCNGG 4 cut(s) 194, 701, 883, 1130
MunI CAATTG 1 cut(s) 1278
Mva1269I GAATGC 1 cut(s) 140
MvaI CCWGG 3 cut(s) 194, 701, 883
MwoI GCNNNNNNNGC 3 cut(s) 227, 236, 1082
NciI CCSGG 1 cut(s) 1130
NdeI CATATG 1 cut(s) 388
NdeII GATC 5 cut(s) 355, 709, 814, 1014, 1135
NlaIII CATG 4 cut(s) 148, 239, 515, 1058
NlaIV GGNNCC 4 cut(s) 115, 394, 1127, 1275
PaeR7I CTCGAG 1 cut(s) 941
PaqCI CACCTGC 1 cut(s) 1255
PasI CCCWGGG 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 553
PctI GAATGC 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 4 cut(s) 268, 537, 717, 1050
PfoI TCCNGGA 1 cut(s) 881
PkrI GCNGC 1 cut(s) 229
PpuMI RGGWCCY 1 cut(s) 1220
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 2 cut(s) 310, 565
Psp5II RGGWCCY 1 cut(s) 1220
Psp6I CCWGG 3 cut(s) 192, 699, 881
PspGI CCWGG 3 cut(s) 192, 699, 881
PspN4I GGNNCC 4 cut(s) 115, 394, 1127, 1275
PspPI GGNCC 2 cut(s) 393, 1220
PspPPI RGGWCCY 1 cut(s) 1220
PstI CTGCAG 1 cut(s) 1228
PsuI RGATCY 1 cut(s) 1014
RseI CAYNNNNRTG 1 cut(s) 516
SacI GAGCTC 2 cut(s) 310, 565
SapI GCTCTTC 1 cut(s) 553
SaqAI TTAA 3 cut(s) 275, 480, 1259
SatI GCNGC 1 cut(s) 228
Sau3AI GATC 5 cut(s) 355, 709, 814, 1014, 1135
Sau96I GGNCC 2 cut(s) 393, 1220
SbfI CCTGCAGG 1 cut(s) 1228
ScaI AGTACT 2 cut(s) 124, 454
ScrFI CCNGG 4 cut(s) 194, 701, 883, 1130
SdaI CCTGCAGG 1 cut(s) 1228
SduI GDGCHC 2 cut(s) 310, 565
SfaNI GCATC 2 cut(s) 1063, 1125
SfcI CTRYAG 2 cut(s) 837, 1224
Sfr274I CTCGAG 1 cut(s) 941
SinI GGWCC 2 cut(s) 393, 1220
SlaI CTCGAG 1 cut(s) 941
SmiMI CAYNNNNRTG 1 cut(s) 516
SmlI CTYRAG 2 cut(s) 941, 1215
SmoI CTYRAG 2 cut(s) 941, 1215
SpeI ACTAGT 2 cut(s) 119, 449
Sse8387I CCTGCAGG 1 cut(s) 1228
Sse9I AATT 9 cut(s) 61, 272, 279, 371, 628, 650, 684, 1080, 1278
SspMI CTAG 5 cut(s) 120, 450, 668, 843, 1028
SstI GAGCTC 2 cut(s) 310, 565
StyD4I CCNGG 4 cut(s) 192, 699, 881, 1128
StyI CCWWGG 3 cut(s) 379, 637, 810
TaaI ACNGT 1 cut(s) 397
TaqI TCGA 1 cut(s) 942
TasI AATT 9 cut(s) 61, 272, 279, 371, 628, 650, 684, 1080, 1278
TatI WGTACW 4 cut(s) 65, 122, 205, 452
TfiI GAWTC 4 cut(s) 268, 537, 717, 1050
Tru1I TTAA 3 cut(s) 275, 480, 1259
Tru9I TTAA 3 cut(s) 275, 480, 1259
TscAI CASTG 1 cut(s) 600
TseI GCWGC 1 cut(s) 227
TspDTI ATGAA 7 cut(s) 161, 189, 224, 498, 595, 600, 1163
TspRI CASTG 1 cut(s) 600
VpaK11BI GGWCC 2 cut(s) 393, 1220
XcmI CCANNNNNNNNNTGG 1 cut(s) 386
XhoI CTCGAG 1 cut(s) 941
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 5 cut(s) 120, 450, 668, 843, 1028
ZrmI AGTACT 2 cut(s) 124, 454
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.