MD02G1245600.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000107
Physical Location & Seq
Reverse (-)
29609922 .. 29612107
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1245600.v1.1.491

Sequence Viewer

Length: 1668 bp
ATGCAGCAGCAAGAGCTCGATATCTCTTGGCCTCGCATATTCTTAATTGCTTTGTTTACAATTCTCAGAGCAACCCAAAATGCGTATACGTGCACGGAGTCCGAATGCGGCAATCATGGCAAGGCTATCCATTTCCCGTTTCACCGCAGACATCACTGTGGTCGGCCAACTGATTGCCATGCGAATGAGGGTGACGGCGGACAGGAGCACCGAGTGGTAGTGATGAAATTCTTGGTGAAACGCATAGATTACAAAAATCAGGAAGTCCAAGTCCATAAACCGTATAATTGCCTGCTACTAAAGCCTTTAGAAGTTGACAACATCAACATGCCATTGACAGCCTCTCAATTCTACTTCCTCCCCTTTTTTACCAGAAACTATACCTTATTCCACTGTCCTGTTGAAAGAGAATTAGACACACCAGTTCCCTGCATAGGTGGCCCTGGCTACAGAGTGTATGCCCTTTCATCTGACTATTTTCTTTGGGACTCGCTGGCCCTCGAGTCCTGTACAAAGATGTACGATGTTTTGTCGGTTCCAAGTGCGGGCGCGAATGGTGCATCCCTGGGATCATTTGCTGTATTAGTACTGGTTGTTGCAGCCTATCGTGTCTATAGTTCTAATCGAAAAGAAAAGGAGAGCCAATTAAAGATTGACAAGTTTTTAGAAGATTACAAAGCTCACAAACCAAGCAGGTATTCCTACGCGGACATTAAGAGGATCACAAACCAATTCCAGGACAAGTTAGGCCAAGGAGCCTATGGAACTGTTTTCAAAGGAAAGCTTTCTTCTGAATGCTTTGTCGCTGTAAAAGTTCTCAATAGTTCCAACAGAAACGGGGAAGATTTCATAAAGGAAGTTGGAATGATGGGACGTATCCACCATGTCAACGTGGTTCGTTTGGTTAGTTTCTGTGCTGATCGATTTAACGGAGCTCTCGTTTATGAGTTCTTCGCCAATGGTTCACTGCACTATTTCATTTCATCGGCAGACAATAACAATCGTTTTCTTGGTTGGGACAAGTTGCAAGATATTGCTCTTGCTGTAGCCAAGGGAATTTATTATCTTCATCAGGAATGTGATCAACGAATCCTCCATTTCGACATCAAGCCTCATAATGTTTTATTAGACGACAATTTCACTCCAAAAATTTCTGATTTTGGTTTGGCCAAGTTGTGTTCTAAGGATCAAAGCATAGTGTCCATGACTATAGCTAGAGGGACTATGGGCTACATTGCACCTGAAGTGTTCTCCAGGAACTTCGGAAATGTGTCCTATAAGTCGGACGTCTATAGTTTTGGAATGCTGCTGCTTGAGATGGTAGGGGGAAGGAAGAATATTGATCCAACCTCAGCAATCCCAACTGAAATTTACTATCCAGAATGGATCTATAATCTTCTAGAAGAAGGAGAGGACCTAAGAATCCATCTCGGGGAGGATGGAGATGGTAAAACTCCAAAGAAACTCGCAATTGTAGGGCTCTGGTGCATCCAGTGGCAGCCAGCGGGTCGTCCTTCCATGAAAGAAGTTGTTCAAATGCTGGAAGGAGGAGAAATCTTAACCATGCCTCCCAATCCTTTTGCATCTACAGGTCCTGCACGAACAAATGCAACTACACCTGCAAGAAATCTAAAAATTCAGTTAGAAGCTATTGCTGAATTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

556

Amino Acids

62.69

Weight (kDa)

6.79

Isoelectric Point (pI)

37.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 244 - 514 7.2e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 246 - 512 9.1e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1627
AatII GACGTC 1 cut(s) 1290
Acc36I ACCTGC 2 cut(s) 684, 1627
AccI GTMKAC 1 cut(s) 86
AccII CGCG 2 cut(s) 551, 707
AciI CCGC 6 cut(s) 108, 145, 198, 545, 707, 1505
AclWI GGATC 5 cut(s) 577, 728, 1194, 1337, 1394
AcoI YGGCCR 2 cut(s) 164, 1167
AcsI RAATTY 5 cut(s) 227, 1056, 1149, 1368, 1635
AcuI CTGAAG 1 cut(s) 1263
AcyI GRCGYC 1 cut(s) 1287
AdeI CACNNNGTG 1 cut(s) 214
AfaI GTAC 3 cut(s) 511, 521, 588
AfiI CCNNNNNNNGG 4 cut(s) 434, 545, 736, 1432
AgsI TTSAA 3 cut(s) 404, 775, 1535
AjnI CCWGG 4 cut(s) 442, 564, 735, 1253
AjuI GAANNNNNNNTTGG 2 cut(s) 682, 714
AluBI AGCT 6 cut(s) 16, 680, 784, 935, 1214, 1649
AluI AGCT 6 cut(s) 16, 680, 784, 935, 1214, 1649
Alw21I GWGCWC 4 cut(s) 18, 95, 210, 937
Alw44I GTGCAC 1 cut(s) 91
AlwI GGATC 5 cut(s) 577, 728, 1194, 1337, 1394
AlwNI CAGNNNCTG 1 cut(s) 1595
Ama87I CYCGRG 2 cut(s) 500, 1430
AoxI GGCC 6 cut(s) 29, 164, 439, 495, 748, 1167
ApaLI GTGCAC 1 cut(s) 91
ApeKI GCWGC 6 cut(s) 4, 7, 599, 1306, 1309, 1498
ApoI RAATTY 5 cut(s) 227, 1056, 1149, 1368, 1635
Asp700I GAANNNNTTC 2 cut(s) 784, 1530
AspLEI GCGC 1 cut(s) 551
AspS9I GGNCC 4 cut(s) 440, 496, 1414, 1592
AsuHPI GGTGA 3 cut(s) 134, 203, 247
AvaI CYCGRG 2 cut(s) 500, 1430
AvaII GGWCC 2 cut(s) 1414, 1592
BaeGI GKGCMC 1 cut(s) 95
BalI TGGCCA 1 cut(s) 1169
BanII GRGCYC 3 cut(s) 18, 937, 1482
Bbv12I GWGCWC 4 cut(s) 18, 95, 210, 937
BbvCI CCTCAGC 1 cut(s) 1351
BbvI GCAGC 6 cut(s) 16, 19, 611, 1293, 1296, 1510
BccI CCATC 5 cut(s) 862, 1312, 1433, 1434, 1439
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 4 cut(s) 444, 566, 737, 1255
BciVI GTATCC 1 cut(s) 887
BclI TGATCA 1 cut(s) 1081
BfaI CTAG 2 cut(s) 1215, 1400
BfmI CTRYAG 6 cut(s) 448, 613, 1044, 1209, 1291, 1587
BfuAI ACCTGC 2 cut(s) 684, 1627
BfuI GTATCC 1 cut(s) 887
BisI GCNGC 7 cut(s) 5, 8, 109, 600, 1307, 1310, 1499
BlsI GCNGC 7 cut(s) 6, 9, 110, 601, 1308, 1311, 1500
BmcAI AGTACT 1 cut(s) 588
Bme1390I CCNGG 4 cut(s) 444, 566, 737, 1255
Bme18I GGWCC 2 cut(s) 1414, 1592
BmeT110I CYCGRG 2 cut(s) 500, 1430
BmgT120I GGNCC 4 cut(s) 440, 496, 1414, 1592
BmiI GGNNCC 2 cut(s) 537, 757
BmrFI CCNGG 4 cut(s) 444, 566, 737, 1255
BmsI GCATC 3 cut(s) 569, 1497, 1592
BpmI CTGGAG 1 cut(s) 1237
Bpu10I CCTNAGC 1 cut(s) 1351
BpuEI CTTGAG 1 cut(s) 1334
Bsa29I ATCGAT 1 cut(s) 922
BsaAI YACGTR 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 1287
BsaJI CCNNGG 5 cut(s) 442, 564, 565, 751, 1050
BsaXI ACNNNNNCTCC 6 cut(s) 924, 954, 1077, 1107, 1552, 1582
Bsc4I CCNNNNNNNGG 4 cut(s) 434, 545, 736, 1432
Bse1I ACTGG 3 cut(s) 422, 594, 1492
Bse3DI GCAATG 1 cut(s) 1233
BseBI CCWGG 4 cut(s) 444, 566, 737, 1255
BseCI ATCGAT 1 cut(s) 922
BseDI CCNNGG 5 cut(s) 442, 564, 565, 751, 1050
BseGI GGATG 3 cut(s) 560, 1444, 1488
BseLI CCNNNNNNNGG 4 cut(s) 434, 545, 736, 1432
BseMI GCAATG 1 cut(s) 1233
BseMII CTCAG 2 cut(s) 79, 1365
BseNI ACTGG 3 cut(s) 422, 594, 1492
BseRI GAGGAG 1 cut(s) 1563
BseSI GKGCMC 1 cut(s) 95
BseXI GCAGC 6 cut(s) 16, 19, 611, 1293, 1296, 1510
BsgI GTGCAG 2 cut(s) 953, 1581
Bsh1236I CGCG 2 cut(s) 551, 707
BshFI GGCC 6 cut(s) 31, 166, 441, 497, 750, 1169
BshVI ATCGAT 1 cut(s) 922
BsiHKAI GWGCWC 4 cut(s) 18, 95, 210, 937
BsiHKCI CYCGRG 2 cut(s) 500, 1430
BslFI GGGAC 4 cut(s) 500, 885, 1031, 1234
BslI CCNNNNNNNGG 4 cut(s) 434, 545, 736, 1432
BsmFI GGGAC 4 cut(s) 500, 885, 1031, 1234
BsmI GAATGC 3 cut(s) 110, 800, 1308
BsnI GGCC 6 cut(s) 31, 166, 441, 497, 750, 1169
BsoBI CYCGRG 2 cut(s) 500, 1430
Bsp1286I GDGCHC 5 cut(s) 18, 95, 210, 937, 1482
Bsp1407I TGTACA 1 cut(s) 509
Bsp143I GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
BspACI CCGC 6 cut(s) 108, 145, 198, 545, 707, 1505
BspANI GGCC 6 cut(s) 31, 166, 441, 497, 750, 1169
BspCNI CTCAG 2 cut(s) 78, 1364
BspDI ATCGAT 1 cut(s) 922
BspFNI CGCG 2 cut(s) 551, 707
BspLI GGNNCC 2 cut(s) 537, 757
BspMI ACCTGC 2 cut(s) 684, 1627
BspPI GGATC 5 cut(s) 577, 728, 1194, 1337, 1394
BsrDI GCAATG 1 cut(s) 1233
BsrGI TGTACA 1 cut(s) 509
BsrI ACTGG 3 cut(s) 422, 594, 1492
BssECI CCNNGG 5 cut(s) 442, 564, 565, 751, 1050
BssMI GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
BssNAI GTATAC 1 cut(s) 87
BssNI GRCGYC 1 cut(s) 1287
BssT1I CCWWGG 2 cut(s) 751, 1050
Bst1107I GTATAC 1 cut(s) 87
Bst2UI CCWGG 4 cut(s) 444, 566, 737, 1255
Bst4CI ACNGT 4 cut(s) 158, 282, 395, 769
BstACI GRCGYC 1 cut(s) 1287
BstAUI TGTACA 1 cut(s) 509
BstBAI YACGTR 1 cut(s) 90
BstC8I GCNNGC 4 cut(s) 293, 495, 547, 1503
BstDEI CTNAG 4 cut(s) 65, 1182, 1351, 1418
BstF5I GGATG 3 cut(s) 560, 1444, 1488
BstFNI CGCG 2 cut(s) 551, 707
BstHHI GCGC 1 cut(s) 551
BstKTI GATC 7 cut(s) 572, 723, 922, 1084, 1189, 1345, 1389
BstMBI GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
BstMWI GCNNNNNNNGC 5 cut(s) 13, 117, 301, 438, 557
BstNI CCWGG 4 cut(s) 444, 566, 737, 1255
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 4 cut(s) 442, 564, 735, 1253
BstSFI CTRYAG 6 cut(s) 448, 613, 1044, 1209, 1291, 1587
BstSLI GKGCMC 1 cut(s) 95
BstUI CGCG 2 cut(s) 551, 707
BstV1I GCAGC 6 cut(s) 16, 19, 611, 1293, 1296, 1510
BstX2I RGATCY 1 cut(s) 1386
BstYI RGATCY 1 cut(s) 1386
BstZ17I GTATAC 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 922
BsuI GTATCC 1 cut(s) 887
BsuRI GGCC 6 cut(s) 31, 166, 441, 497, 750, 1169
BsuTUI ATCGAT 1 cut(s) 922
BtsCI GGATG 3 cut(s) 560, 1444, 1488
BtsI GCAGTG 1 cut(s) 965
BtsIMutI CAGTG 4 cut(s) 154, 391, 965, 1499
BveI ACCTGC 2 cut(s) 684, 1627
Cac8I GCNNGC 4 cut(s) 293, 495, 547, 1503
CaiI CAGNNNCTG 1 cut(s) 1595
CfoI GCGC 1 cut(s) 551
Cfr13I GGNCC 4 cut(s) 440, 496, 1414, 1592
ClaI ATCGAT 1 cut(s) 922
Csp6I GTAC 3 cut(s) 510, 520, 587
CviAII CATG 7 cut(s) 116, 179, 328, 884, 1204, 1519, 1564
CviQI GTAC 3 cut(s) 510, 520, 587
DdeI CTNAG 4 cut(s) 65, 1182, 1351, 1418
DpnI GATC 7 cut(s) 571, 722, 921, 1083, 1188, 1344, 1388
DpnII GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
DraIII CACNNNGTG 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 164, 1167
EciI GGCGGA 1 cut(s) 213
Ecl136II GAGCTC 2 cut(s) 16, 935
Eco130I CCWWGG 2 cut(s) 751, 1050
Eco24I GRGCYC 3 cut(s) 18, 937, 1482
Eco32I GATATC 1 cut(s) 22
Eco47I GGWCC 2 cut(s) 1414, 1592
Eco53kI GAGCTC 2 cut(s) 16, 935
Eco57I CTGAAG 1 cut(s) 1263
Eco88I CYCGRG 2 cut(s) 500, 1430
EcoICRI GAGCTC 2 cut(s) 16, 935
EcoO109I RGGNCCY 2 cut(s) 1414, 1592
EcoRII CCWGG 4 cut(s) 442, 564, 735, 1253
EcoRV GATATC 1 cut(s) 22
EcoT14I CCWWGG 2 cut(s) 751, 1050
EcoT38I GRGCYC 3 cut(s) 18, 937, 1482
ErhI CCWWGG 2 cut(s) 751, 1050
FaeI CATG 7 cut(s) 119, 182, 331, 887, 1207, 1522, 1567
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FaqI GGGAC 4 cut(s) 500, 885, 1031, 1234
FatI CATG 7 cut(s) 115, 178, 327, 883, 1203, 1518, 1563
FauI CCCGC 2 cut(s) 538, 1498
FbaI TGATCA 1 cut(s) 1081
FblI GTMKAC 1 cut(s) 86
Fnu4HI GCNGC 7 cut(s) 5, 8, 109, 600, 1307, 1310, 1499
FokI GGATG 3 cut(s) 547, 1451, 1475
FriOI GRGCYC 3 cut(s) 18, 937, 1482
Fsp4HI GCNGC 7 cut(s) 5, 8, 109, 600, 1307, 1310, 1499
FspBI CTAG 2 cut(s) 1215, 1400
GlaI GCGC 1 cut(s) 550
GluI GCNGC 7 cut(s) 5, 8, 109, 600, 1307, 1310, 1499
GsuI CTGGAG 1 cut(s) 1237
HaeIII GGCC 6 cut(s) 31, 166, 441, 497, 750, 1169
HhaI GCGC 1 cut(s) 551
Hin1I GRCGYC 1 cut(s) 1287
Hin1II CATG 7 cut(s) 119, 182, 331, 887, 1207, 1522, 1567
Hin6I GCGC 1 cut(s) 549
HinP1I GCGC 1 cut(s) 549
HincII GTYRAC 2 cut(s) 316, 889
HindII GTYRAC 2 cut(s) 316, 889
HindIII AAGCTT 1 cut(s) 782
HinfI GANTC 5 cut(s) 98, 488, 503, 1089, 1422
HphI GGTGA 3 cut(s) 134, 203, 247
Hpy166II GTNNAC 6 cut(s) 57, 87, 93, 316, 889, 965
Hpy188I TCNGA 7 cut(s) 68, 103, 472, 793, 1156, 1265, 1285
Hpy188III TCNNGA 4 cut(s) 260, 1073, 1379, 1400
Hpy8I GTNNAC 6 cut(s) 57, 87, 93, 316, 889, 965
HpyAV CCTTC 4 cut(s) 1323, 1400, 1524, 1538
HpyCH4III ACNGT 4 cut(s) 158, 282, 395, 769
HpyCH4IV ACGT 4 cut(s) 89, 874, 891, 1287
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 117, 301, 438, 557
HpyF3I CTNAG 4 cut(s) 65, 1182, 1351, 1418
HpySE526I ACGT 4 cut(s) 89, 874, 891, 1287
Hsp92I GRCGYC 1 cut(s) 1287
Hsp92II CATG 7 cut(s) 119, 182, 331, 887, 1207, 1522, 1567
HspAI GCGC 1 cut(s) 549
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
LmnI GCTCC 3 cut(s) 205, 755, 932
Lsp1109I GCAGC 6 cut(s) 16, 19, 611, 1293, 1296, 1510
LweI GCATC 3 cut(s) 569, 1497, 1592
MaeI CTAG 2 cut(s) 1215, 1400
MaeII ACGT 4 cut(s) 89, 874, 891, 1287
MaeIII GTNAC 1 cut(s) 191
MalI GATC 7 cut(s) 571, 722, 921, 1083, 1188, 1344, 1388
MboI GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
MboII GAAGA 8 cut(s) 680, 780, 854, 943, 1058, 1345, 1388, 1415
MfeI CAATTG 1 cut(s) 1470
MflI RGATCY 1 cut(s) 1386
MhlI GDGCHC 5 cut(s) 18, 95, 210, 937, 1482
MlsI TGGCCA 1 cut(s) 1169
MluNI TGGCCA 1 cut(s) 1169
MlyI GAGTC 3 cut(s) 107, 482, 512
MmeI TCCRAC 4 cut(s) 841, 852, 1263, 1370
Mox20I TGGCCA 1 cut(s) 1169
MroXI GAANNNNTTC 2 cut(s) 784, 1530
MscI TGGCCA 1 cut(s) 1169
MseI TTAA 5 cut(s) 44, 647, 714, 927, 1559
MslI CAYNNNNRTG 3 cut(s) 156, 183, 326
Msp20I TGGCCA 1 cut(s) 1169
MspA1I CMGCKG 1 cut(s) 1505
MspR9I CCNGG 4 cut(s) 444, 566, 737, 1255
MunI CAATTG 1 cut(s) 1470
Mva1269I GAATGC 3 cut(s) 110, 800, 1308
MvaI CCWGG 4 cut(s) 444, 566, 737, 1255
MvnI CGCG 2 cut(s) 551, 707
MwoI GCNNNNNNNGC 5 cut(s) 13, 117, 301, 438, 557
NdeII GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
NlaIII CATG 7 cut(s) 119, 182, 331, 887, 1207, 1522, 1567
NlaIV GGNNCC 2 cut(s) 537, 757
NmuCI GTSAC 1 cut(s) 191
NspI RCATGY 1 cut(s) 331
PaeR7I CTCGAG 1 cut(s) 500
PaqCI CACCTGC 1 cut(s) 1627
PasI CCCWGGG 1 cut(s) 565
PcsI WCGNNNNNNNCGW 1 cut(s) 936
PctI GAATGC 3 cut(s) 110, 800, 1308
PdmI GAANNNNTTC 2 cut(s) 784, 1530
PfeI GAWTC 2 cut(s) 1089, 1422
PfoI TCCNGGA 2 cut(s) 735, 1253
PkrI GCNGC 7 cut(s) 6, 9, 110, 601, 1308, 1311, 1500
PleI GAGTC 3 cut(s) 106, 482, 511
PpsI GAGTC 3 cut(s) 106, 482, 511
Ppu21I YACGTR 1 cut(s) 90
PpuMI RGGWCCY 2 cut(s) 1414, 1592
Psp124BI GAGCTC 2 cut(s) 18, 937
Psp5II RGGWCCY 2 cut(s) 1414, 1592
Psp6I CCWGG 4 cut(s) 442, 564, 735, 1253
PspGI CCWGG 4 cut(s) 442, 564, 735, 1253
PspN4I GGNNCC 2 cut(s) 537, 757
PspPI GGNCC 4 cut(s) 440, 496, 1414, 1592
PspPPI RGGWCCY 2 cut(s) 1414, 1592
PspXI VCTCGAGB 1 cut(s) 500
PstNI CAGNNNCTG 1 cut(s) 1595
PsuI RGATCY 1 cut(s) 1386
RsaI GTAC 3 cut(s) 511, 521, 588
RsaNI GTAC 3 cut(s) 510, 520, 587
RseI CAYNNNNRTG 3 cut(s) 156, 183, 326
SacI GAGCTC 2 cut(s) 18, 937
SaqAI TTAA 5 cut(s) 44, 647, 714, 927, 1559
SatI GCNGC 7 cut(s) 5, 8, 109, 600, 1307, 1310, 1499
Sau3AI GATC 7 cut(s) 569, 720, 919, 1081, 1186, 1342, 1386
Sau96I GGNCC 4 cut(s) 440, 496, 1414, 1592
ScaI AGTACT 1 cut(s) 588
SchI GAGTC 3 cut(s) 107, 482, 512
ScrFI CCNGG 4 cut(s) 444, 566, 737, 1255
SduI GDGCHC 5 cut(s) 18, 95, 210, 937, 1482
SfaNI GCATC 3 cut(s) 569, 1497, 1592
SfcI CTRYAG 6 cut(s) 448, 613, 1044, 1209, 1291, 1587
Sfr274I CTCGAG 1 cut(s) 500
SinI GGWCC 2 cut(s) 1414, 1592
SlaI CTCGAG 1 cut(s) 500
SmiMI CAYNNNNRTG 3 cut(s) 156, 183, 326
SmlI CTYRAG 2 cut(s) 500, 1313
SmoI CTYRAG 2 cut(s) 500, 1313
SsiI CCGC 6 cut(s) 108, 145, 198, 545, 707, 1505
SspI AATATT 1 cut(s) 1339
SspMI CTAG 2 cut(s) 1215, 1400
SstI GAGCTC 2 cut(s) 18, 937
StyD4I CCNGG 4 cut(s) 442, 564, 735, 1253
StyI CCWWGG 2 cut(s) 751, 1050
TaaI ACNGT 4 cut(s) 158, 282, 395, 769
TaiI ACGT 4 cut(s) 92, 877, 894, 1290
TaqI TCGA 5 cut(s) 18, 501, 625, 922, 1101
TatI WGTACW 2 cut(s) 509, 586
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 2 cut(s) 1089, 1422
Tru1I TTAA 5 cut(s) 44, 647, 714, 927, 1559
Tru9I TTAA 5 cut(s) 44, 647, 714, 927, 1559
TscAI CASTG 4 cut(s) 161, 398, 972, 1499
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 6 cut(s) 4, 7, 599, 1306, 1309, 1498
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 7 cut(s) 239, 456, 838, 967, 972, 1058, 1535
TspGWI ACGGA 2 cut(s) 110, 945
TspRI CASTG 4 cut(s) 161, 398, 972, 1499
VneI GTGCAC 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 1414, 1592
XapI RAATTY 5 cut(s) 227, 1056, 1149, 1368, 1635
XbaI TCTAGA 1 cut(s) 1399
XceI RCATGY 1 cut(s) 331
XcmI CCANNNNNNNNNTGG 1 cut(s) 758
XhoI CTCGAG 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 86
XmnI GAANNNNTTC 2 cut(s) 784, 1530
XspI CTAG 2 cut(s) 1215, 1400
ZraI GACGTC 1 cut(s) 1288
ZrmI AGTACT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.