MD02G1250400.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000107
Physical Location & Seq
Forward (+)
30200636 .. 30202930
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1250400.v1.1.491

Sequence Viewer

Length: 1794 bp
ATGCAGAAATTGATCATCTCTTACTTTTGCATATTGTTACTGGTTTTCTTCAAACTAGTTGGAGCAAGCCATGCGTGCCTGGAATCCAAGTGTAGCGATGATCAAGGCGCAGCTATCCATTTCCCATTGCGCATCAGCCGCAAACATCATTGTGGAAATCCTGGGCTTGAATGCAATGTAAGATATGAGCAGCAAATACTAGTAAAATTATTTGTCAAACACATAGATTACAAGCGTCAGGAAATCCAAGTGTATCCCCCAAGTGATTGCCTGCTGCTGAAGCCTGTCGAAGTCACAAACATAGATTACAAGCGTCAGGAAATCCTAGTGTCTCCCCCAAGTAACAACTTGTCATATTCTTTCATCAGAAACTTAACCTTATTCAGTTGTCCTACTTCTGTTGCAAGAGATACATATGCGTATCAAGTCCACTGCCTCGGTGACCCTGGCAGCAGAATTTATGCCCTTGATTCTACATATCAATTAGAAAATCTCTTCGAGAATCTACAATCTTGTTCAAAGATGTATGATGTTTTATCTGGCAATTGGGAGGGTAATGGTTCTGTTCTTCAATTCAAATGGTCAAAACCAAATTGTACAGAATGTGAAGCGGAGGGAAAGATGTGTAGATGGAAGAACAATGGCACCAACAGTGAAATTGAATGTCAACGCGTGAGGAAACCAAGCAACACATGGAAATTAGTGGTTACAGGTGGAATTCTGGGTGCTTTGCTTCTTGTTCTACTGATGATTGCAGCATATCGGGTATATAGCACTGATAGGAAGGAAAAAGAGAGTCGATTAAGAATTGAAAGATTCTTGGAGGATTATAAAGCACTCAAACCAAGCAGGTATTCGTACGCAGATATTAAGAGGATTACAAATCAATTCAAGGACAAGTTGGGCGAAGGAGCCTATGGAACTGTCTTTAAAGGAATGCTCTCTTCTGAATTCTTTATTGCTGTCAAAGTCCTCAACAGTTCAAAGGGAGATGGGGAAGAGTTCGTAAATGAAGTGCGAACGATGGGTCATATCCACCATGTCAACGTGGCTCGCTTGGTTGGATTTTGTGCTGATGGATTTGTACGAGCTCTTGTTTCCCTGCAGGATTTTATTTCATCTGCAGATAGTAAGAATTCACTTCTTGGTTGGGATAAGCTGCAAGATATTGCCCTAGGCATAGCCAAAGGAATTGAATATCTTCACCAAGGATGCGATCTACGAATCCTGCATTTCGATATCAAGCCCCATAATGTGTTGCTAGACCAAAACTTCACTCCAAAAATTTCTGATTTTGGTTTGGCCAAGTTATGTTCCAAGGATCAAAGCTTGGTGTCCATAACTACAGCTAGGGGGACCATGGGCTATATTGCACCTGAAGTGTTCTCCAGGAATTTCGGAAATGTGTCTTACAAGGCAGATGTCTACAGCTTTGGAATGCTGCTGCTTGAGATGGTAGGAGGAAGGAAGAATATTGGTTCAACCACGGAGAACACTACAAATGAAATCTACTATCCACAGTGGATTTACAATCTTCTAGAGGAAGGGGATGACCTACGAATCCGTATTGGGCAAGAAGAAGATGGTAAAATTCCAAAGAAACTTGCTATTATAGGGTTATGGTGTATCCAATGGCACCCGTCGGATCGTCTTTCCATGAAAACAGCCGTTCAGATGTTAGAAGGAGGAGGAGGAGAAAGCTTGACCATGCCGCCTAATCCCTTCATGCCTACAGGTCCTACAACTAGAAATGCAAGACGCCTAGAGCGAGAAGCAATTGCTGAATTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

598

Amino Acids

67.49

Weight (kDa)

8.18

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 296 - 559 1.8e-35 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 297 - 558 3.1e-40 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 831
Acc16I TGCGCA 1 cut(s) 131
Acc36I ACCTGC 1 cut(s) 840
AccB1I GGYRCC 2 cut(s) 644, 1635
AccI GTMKAC 1 cut(s) 1425
AccII CGCG 1 cut(s) 672
AciI CCGC 3 cut(s) 139, 611, 1712
AclWI GGATC 2 cut(s) 1329, 1653
AcoI YGGCCR 1 cut(s) 1302
AcsI RAATTY 7 cut(s) 456, 717, 950, 1135, 1284, 1393, 1590
AcuI CTGAAG 2 cut(s) 299, 1398
AcyI GRCGYC 1 cut(s) 1759
AfaI GTAC 3 cut(s) 598, 860, 1086
AflIII ACRYGT 1 cut(s) 670
AhlI ACTAGT 2 cut(s) 55, 199
AjnI CCWGG 4 cut(s) 78, 160, 445, 1388
AjuI GAANNNNNNNTTGG 2 cut(s) 838, 870
AluBI AGCT 7 cut(s) 113, 1091, 1159, 1329, 1349, 1431, 1701
AluI AGCT 7 cut(s) 113, 1091, 1159, 1329, 1349, 1431, 1701
Alw21I GWGCWC 1 cut(s) 1093
Alw26I GTCTC 1 cut(s) 336
AlwI GGATC 2 cut(s) 1329, 1653
AoxI GGCC 1 cut(s) 1302
ApeKI GCWGC 8 cut(s) 110, 190, 274, 450, 755, 1159, 1441, 1444
ApoI RAATTY 7 cut(s) 456, 717, 950, 1135, 1284, 1393, 1590
ArsI GACNNNNNNTTYG 2 cut(s) 1012, 1044
Asp700I GAANNNNTTC 1 cut(s) 1200
AspA2I CCTAGG 1 cut(s) 1174
AspLEI GCGC 2 cut(s) 110, 132
AspS9I GGNCC 2 cut(s) 1356, 1736
AsuHPI GGTGA 2 cut(s) 452, 1196
AvaII GGWCC 2 cut(s) 1356, 1736
AvrII CCTAGG 1 cut(s) 1174
BalI TGGCCA 1 cut(s) 1304
BanI GGYRCC 2 cut(s) 644, 1635
BanII GRGCYC 1 cut(s) 1093
Bbv12I GWGCWC 1 cut(s) 1093
BbvI GCAGC 8 cut(s) 122, 202, 261, 462, 767, 1146, 1428, 1431
BccI CCATC 6 cut(s) 624, 986, 1018, 1070, 1447, 1577
BceAI ACGGC 1 cut(s) 1652
BciT130I CCWGG 4 cut(s) 80, 162, 447, 1390
BciVI GTATCC 2 cut(s) 264, 1637
BclI TGATCA 2 cut(s) 12, 100
BcoDI GTCTC 1 cut(s) 336
BcuI ACTAGT 2 cut(s) 55, 199
BfaI CTAG 9 cut(s) 56, 200, 326, 1175, 1262, 1350, 1538, 1746, 1763
BfmI CTRYAG 5 cut(s) 1103, 1122, 1344, 1426, 1731
BfuAI ACCTGC 1 cut(s) 840
BfuI GTATCC 2 cut(s) 264, 1637
BlnI CCTAGG 1 cut(s) 1174
Bme1390I CCNGG 4 cut(s) 80, 162, 447, 1390
Bme18I GGWCC 2 cut(s) 1356, 1736
BmgT120I GGNCC 2 cut(s) 1356, 1736
BmiI GGNNCC 4 cut(s) 646, 913, 1357, 1637
BmrFI CCNGG 4 cut(s) 80, 162, 447, 1390
BmsI GCATC 2 cut(s) 141, 1202
BpmI CTGGAG 1 cut(s) 1372
BpuEI CTTGAG 1 cut(s) 1469
BsaHI GRCGYC 1 cut(s) 1759
BsaJI CCNNGG 8 cut(s) 161, 436, 445, 1174, 1207, 1317, 1359, 1485
Bse1I ACTGG 1 cut(s) 45
Bse3DI GCAATG 2 cut(s) 125, 181
BseBI CCWGG 4 cut(s) 80, 162, 447, 1390
BseDI CCNNGG 8 cut(s) 161, 436, 445, 1174, 1207, 1317, 1359, 1485
BseGI GGATG 2 cut(s) 1217, 1555
BseMI GCAATG 2 cut(s) 125, 181
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 3 cut(s) 1701, 1704, 1707
BseXI GCAGC 8 cut(s) 122, 202, 261, 462, 767, 1146, 1428, 1431
Bsh1236I CGCG 1 cut(s) 672
BshFI GGCC 1 cut(s) 1304
BshNI GGYRCC 2 cut(s) 644, 1635
BsiHKAI GWGCWC 1 cut(s) 1093
BsiWI CGTACG 1 cut(s) 858
BslFI GGGAC 1 cut(s) 1369
BsmAI GTCTC 1 cut(s) 336
BsmFI GGGAC 1 cut(s) 1369
BsmI GAATGC 3 cut(s) 176, 942, 1443
BsnI GGCC 1 cut(s) 1304
Bsp1286I GDGCHC 1 cut(s) 1093
Bsp1407I TGTACA 1 cut(s) 596
Bsp143I GATC 5 cut(s) 12, 100, 1216, 1321, 1645
Bsp19I CCATGG 1 cut(s) 1359
BspACI CCGC 3 cut(s) 139, 611, 1712
BspANI GGCC 1 cut(s) 1304
BspFNI CGCG 1 cut(s) 672
BspLI GGNNCC 4 cut(s) 646, 913, 1357, 1637
BspMAI CTGCAG 2 cut(s) 1107, 1126
BspMI ACCTGC 1 cut(s) 840
BspPI GGATC 2 cut(s) 1329, 1653
BspT107I GGYRCC 2 cut(s) 644, 1635
BsrDI GCAATG 2 cut(s) 125, 181
BsrGI TGTACA 1 cut(s) 596
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 8 cut(s) 161, 436, 445, 1174, 1207, 1317, 1359, 1485
BssMI GATC 5 cut(s) 12, 100, 1216, 1321, 1645
BssNI GRCGYC 1 cut(s) 1759
BssT1I CCWWGG 4 cut(s) 1174, 1207, 1317, 1359
Bst2UI CCWGG 4 cut(s) 80, 162, 447, 1390
Bst4CI ACNGT 4 cut(s) 653, 925, 980, 1521
Bst6I CTCTTC 3 cut(s) 500, 949, 993
BstACI GRCGYC 1 cut(s) 1759
BstAPI GCANNNNNTGC 1 cut(s) 71
BstAUI TGTACA 1 cut(s) 596
BstC8I GCNNGC 4 cut(s) 67, 76, 272, 1054
BstDSI CCRYGG 2 cut(s) 1359, 1485
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 2 cut(s) 1217, 1555
BstFNI CGCG 1 cut(s) 672
BstHHI GCGC 2 cut(s) 110, 132
BstKTI GATC 5 cut(s) 15, 103, 1219, 1324, 1648
BstMAI GTCTC 1 cut(s) 336
BstMBI GATC 5 cut(s) 12, 100, 1216, 1321, 1645
BstMWI GCNNNNNNNGC 4 cut(s) 71, 75, 138, 280
BstNI CCWGG 4 cut(s) 80, 162, 447, 1390
BstPI GGTNACC 1 cut(s) 440
BstSCI CCNGG 4 cut(s) 78, 160, 445, 1388
BstSFI CTRYAG 5 cut(s) 1103, 1122, 1344, 1426, 1731
BstUI CGCG 1 cut(s) 672
BstV1I GCAGC 8 cut(s) 122, 202, 261, 462, 767, 1146, 1428, 1431
BsuI GTATCC 2 cut(s) 264, 1637
BsuRI GGCC 1 cut(s) 1304
BtgI CCRYGG 2 cut(s) 1359, 1485
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 1217, 1555
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 4 cut(s) 430, 658, 774, 1526
BveI ACCTGC 1 cut(s) 840
Cac8I GCNNGC 4 cut(s) 67, 76, 272, 1054
CfoI GCGC 2 cut(s) 110, 132
Cfr13I GGNCC 2 cut(s) 1356, 1736
CseI GACGC 3 cut(s) 224, 302, 1767
Csp6I GTAC 3 cut(s) 597, 859, 1085
CviAII CATG 7 cut(s) 71, 693, 1040, 1360, 1657, 1708, 1726
CviQI GTAC 3 cut(s) 597, 859, 1085
DpnI GATC 5 cut(s) 14, 102, 1218, 1323, 1647
DpnII GATC 5 cut(s) 12, 100, 1216, 1321, 1645
DraI TTTAAA 1 cut(s) 931
EaeI YGGCCR 1 cut(s) 1302
Eam1104I CTCTTC 3 cut(s) 500, 949, 993
EarI CTCTTC 3 cut(s) 500, 949, 993
Ecl136II GAGCTC 1 cut(s) 1091
Eco130I CCWWGG 4 cut(s) 1174, 1207, 1317, 1359
Eco24I GRGCYC 1 cut(s) 1093
Eco32I GATATC 1 cut(s) 1240
Eco47I GGWCC 2 cut(s) 1356, 1736
Eco53kI GAGCTC 1 cut(s) 1091
Eco57I CTGAAG 2 cut(s) 299, 1398
Eco91I GGTNACC 1 cut(s) 440
EcoICRI GAGCTC 1 cut(s) 1091
EcoO109I RGGNCCY 1 cut(s) 1736
EcoO65I GGTNACC 1 cut(s) 440
EcoRI GAATTC 3 cut(s) 717, 950, 1135
EcoRII CCWGG 4 cut(s) 78, 160, 445, 1388
EcoRV GATATC 1 cut(s) 1240
EcoT14I CCWWGG 4 cut(s) 1174, 1207, 1317, 1359
EcoT38I GRGCYC 1 cut(s) 1093
ErhI CCWWGG 4 cut(s) 1174, 1207, 1317, 1359
FaeI CATG 7 cut(s) 74, 696, 1043, 1363, 1660, 1711, 1729
FaqI GGGAC 1 cut(s) 1369
FatI CATG 7 cut(s) 70, 692, 1039, 1359, 1656, 1707, 1725
FauNDI CATATG 1 cut(s) 415
FbaI TGATCA 2 cut(s) 12, 100
FblI GTMKAC 1 cut(s) 1425
FokI GGATG 2 cut(s) 1224, 1562
FriOI GRGCYC 1 cut(s) 1093
FspBI CTAG 9 cut(s) 56, 200, 326, 1175, 1262, 1350, 1538, 1746, 1763
FspI TGCGCA 1 cut(s) 131
GlaI GCGC 2 cut(s) 109, 131
GsuI CTGGAG 1 cut(s) 1372
HaeIII GGCC 1 cut(s) 1304
HgaI GACGC 3 cut(s) 224, 302, 1767
HhaI GCGC 2 cut(s) 110, 132
Hin1I GRCGYC 1 cut(s) 1759
Hin1II CATG 7 cut(s) 74, 696, 1043, 1363, 1660, 1711, 1729
Hin6I GCGC 2 cut(s) 108, 130
HinP1I GCGC 2 cut(s) 108, 130
HincII GTYRAC 2 cut(s) 668, 1045
HindII GTYRAC 2 cut(s) 668, 1045
HindIII AAGCTT 2 cut(s) 1327, 1699
HinfI GANTC 7 cut(s) 83, 470, 502, 796, 816, 1224, 1560
HphI GGTGA 2 cut(s) 452, 1196
Hpy166II GTNNAC 4 cut(s) 430, 668, 1045, 1426
Hpy188I TCNGA 6 cut(s) 368, 949, 1291, 1400, 1645, 1674
Hpy188III TCNNGA 4 cut(s) 239, 317, 499, 1538
Hpy8I GTNNAC 4 cut(s) 430, 668, 1045, 1426
Hpy99I CGWCG 1 cut(s) 1645
HpyAV CCTTC 6 cut(s) 778, 902, 1458, 1538, 1676, 1732
HpyCH4III ACNGT 4 cut(s) 653, 925, 980, 1521
HpyCH4IV ACGT 1 cut(s) 1047
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 75, 138, 280
HpySE526I ACGT 1 cut(s) 1047
Hsp92I GRCGYC 1 cut(s) 1759
Hsp92II CATG 7 cut(s) 74, 696, 1043, 1363, 1660, 1711, 1729
HspAI GCGC 2 cut(s) 108, 130
Ksp22I TGATCA 2 cut(s) 12, 100
Kzo9I GATC 5 cut(s) 12, 100, 1216, 1321, 1645
LmnI GCTCC 2 cut(s) 62, 911
Lsp1109I GCAGC 8 cut(s) 122, 202, 261, 462, 767, 1146, 1428, 1431
LweI GCATC 2 cut(s) 141, 1202
MaeI CTAG 9 cut(s) 56, 200, 326, 1175, 1262, 1350, 1538, 1746, 1763
MaeII ACGT 1 cut(s) 1047
MaeIII GTNAC 5 cut(s) 36, 292, 341, 440, 706
MalI GATC 5 cut(s) 14, 102, 1218, 1323, 1647
MboI GATC 5 cut(s) 12, 100, 1216, 1321, 1645
MfeI CAATTG 2 cut(s) 544, 1776
MhlI GDGCHC 1 cut(s) 1093
MlsI TGGCCA 1 cut(s) 1304
MluI ACGCGT 1 cut(s) 670
MluNI TGGCCA 1 cut(s) 1304
MlyI GAGTC 1 cut(s) 805
MmeI TCCRAC 3 cut(s) 40, 1042, 1623
Mox20I TGGCCA 1 cut(s) 1304
MroXI GAANNNNTTC 1 cut(s) 1200
MscI TGGCCA 1 cut(s) 1304
MseI TTAA 4 cut(s) 374, 803, 870, 930
MslI CAYNNNNRTG 1 cut(s) 150
Msp20I TGGCCA 1 cut(s) 1304
MspR9I CCNGG 4 cut(s) 80, 162, 447, 1390
MunI CAATTG 2 cut(s) 544, 1776
Mva1269I GAATGC 3 cut(s) 176, 942, 1443
MvaI CCWGG 4 cut(s) 80, 162, 447, 1390
MvnI CGCG 1 cut(s) 672
MwoI GCNNNNNNNGC 4 cut(s) 71, 75, 138, 280
NcoI CCATGG 1 cut(s) 1359
NdeI CATATG 1 cut(s) 415
NdeII GATC 5 cut(s) 12, 100, 1216, 1321, 1645
NlaIII CATG 7 cut(s) 74, 696, 1043, 1363, 1660, 1711, 1729
NlaIV GGNNCC 4 cut(s) 646, 913, 1357, 1637
NmuCI GTSAC 2 cut(s) 292, 440
NsbI TGCGCA 1 cut(s) 131
PcsI WCGNNNNNNNCGW 1 cut(s) 1765
PctI GAATGC 3 cut(s) 176, 942, 1443
PdmI GAANNNNTTC 1 cut(s) 1200
PfeI GAWTC 6 cut(s) 83, 470, 502, 816, 1224, 1560
Pfl23II CGTACG 1 cut(s) 858
PfoI TCCNGGA 1 cut(s) 1388
PleI GAGTC 1 cut(s) 804
PpsI GAGTC 1 cut(s) 804
PpuMI RGGWCCY 1 cut(s) 1736
PsiI TTATAA 1 cut(s) 831
Psp124BI GAGCTC 1 cut(s) 1093
Psp5II RGGWCCY 1 cut(s) 1736
Psp6I CCWGG 4 cut(s) 78, 160, 445, 1388
PspEI GGTNACC 1 cut(s) 440
PspGI CCWGG 4 cut(s) 78, 160, 445, 1388
PspLI CGTACG 1 cut(s) 858
PspN4I GGNNCC 4 cut(s) 646, 913, 1357, 1637
PspPI GGNCC 2 cut(s) 1356, 1736
PspPPI RGGWCCY 1 cut(s) 1736
PstI CTGCAG 2 cut(s) 1107, 1126
RsaI GTAC 3 cut(s) 598, 860, 1086
RsaNI GTAC 3 cut(s) 597, 859, 1085
RseI CAYNNNNRTG 1 cut(s) 150
SacI GAGCTC 1 cut(s) 1093
SaqAI TTAA 4 cut(s) 374, 803, 870, 930
Sau3AI GATC 5 cut(s) 12, 100, 1216, 1321, 1645
Sau96I GGNCC 2 cut(s) 1356, 1736
SbfI CCTGCAGG 1 cut(s) 1107
SchI GAGTC 1 cut(s) 805
ScrFI CCNGG 4 cut(s) 80, 162, 447, 1390
SdaI CCTGCAGG 1 cut(s) 1107
SduI GDGCHC 1 cut(s) 1093
SfaNI GCATC 2 cut(s) 141, 1202
SfcI CTRYAG 5 cut(s) 1103, 1122, 1344, 1426, 1731
SinI GGWCC 2 cut(s) 1356, 1736
SmiMI CAYNNNNRTG 1 cut(s) 150
SmlI CTYRAG 1 cut(s) 1448
SmoI CTYRAG 1 cut(s) 1448
SpeI ACTAGT 2 cut(s) 55, 199
Sse8387I CCTGCAGG 1 cut(s) 1107
SsiI CCGC 3 cut(s) 139, 611, 1712
SspI AATATT 1 cut(s) 1474
SspMI CTAG 9 cut(s) 56, 200, 326, 1175, 1262, 1350, 1538, 1746, 1763
SstI GAGCTC 1 cut(s) 1093
StyD4I CCNGG 4 cut(s) 78, 160, 445, 1388
StyI CCWWGG 4 cut(s) 1174, 1207, 1317, 1359
TaaI ACNGT 4 cut(s) 653, 925, 980, 1521
TaiI ACGT 1 cut(s) 1050
TaqI TCGA 4 cut(s) 288, 498, 799, 1236
TatI WGTACW 1 cut(s) 596
TauI GCSGC 2 cut(s) 141, 1714
TfiI GAWTC 6 cut(s) 83, 470, 502, 816, 1224, 1560
Tru1I TTAA 4 cut(s) 374, 803, 870, 930
Tru9I TTAA 4 cut(s) 374, 803, 870, 930
TscAI CASTG 4 cut(s) 437, 658, 781, 1526
TseFI GTSAC 2 cut(s) 292, 440
TseI GCWGC 8 cut(s) 110, 190, 274, 450, 755, 1159, 1441, 1444
Tsp45I GTSAC 2 cut(s) 292, 440
TspDTI ATGAA 6 cut(s) 352, 1026, 1107, 1518, 1673, 1714
TspGWI ACGGA 2 cut(s) 1502, 1553
TspRI CASTG 4 cut(s) 437, 658, 781, 1526
VpaK11BI GGWCC 2 cut(s) 1356, 1736
XapI RAATTY 7 cut(s) 456, 717, 950, 1135, 1284, 1393, 1590
XbaI TCTAGA 1 cut(s) 1537
XcmI CCANNNNNNNNNTGG 1 cut(s) 690
XmaJI CCTAGG 1 cut(s) 1174
XmiI GTMKAC 1 cut(s) 1425
XmnI GAANNNNTTC 1 cut(s) 1200
XspI CTAG 9 cut(s) 56, 200, 326, 1175, 1262, 1350, 1538, 1746, 1763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.