MD02G1249800.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000107
Physical Location & Seq
Forward (+)
30072889 .. 30075771
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1249800.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGCCACCCTCTCCCTTCTACTTGACAAATTTTAGAAACCTTAACCTTACCTTATTCAGTTGTCCTACTTCTGTTGCAAGAGATATGGATTTGTATCAAGTCCCCTGCCTCGGCGACCCTAGCAGCAGAATTTATGCCATTGATTCTACATACGAATTAGAAGATTTCTTCCAGAATCTACAATCTTGTTCAAAGATGTATGATGTTTTATCAGTTCCATTTGGCGATTGGGCGGGTAATGGCCGTGTTCTTCAATTCAAATGGTCGAAACCAAATTGTACAGAATGTGAAGCAGAGGGAAAGAGGTGTAAATGGAAGAACAATGGCACCAACAGTGAAATTGAATGTCAACACGTGAGCAAACCAAGCAACACATGGAAATTAGTGGTTACAGGTGGAGTCCTGGGTTCTTTGCTTCTTGTTCTACTGATGATTGCAGCATATCGGGTATATAGAGCTGATAGAAAGGAAAAAGAGAGCCAATTAAGAATTGAAAGATTCTTGGAGGATTACAAAGCACTCAAACCAAGCAGGTATTCGTATGCAGATATTAAGAGGATTACAAATCAATTCAAGGACAAGTTGGGCGAAGGAGCCTATGGAACTGTCTTCAAAGGAATGCTCTCTTCTGAATTCTTTATTGCTGTCAAAGTCCTCAACAGTTCCAAGGGAGATGGGGAAGAGTTCGTAAATGAAGTGCGAACGATGGGTCATATCCACCACGTCAACGTGGCTCGCTTGGTTGGATTTTGTGCTGATGGATTTATACGAGCTCTTGTTTACGAGTTCTTCCCCAACGGTTCCCTGCAGGATTTCATTTCATCAGCAGATAGTAAGAATTCATTTCTTGGTTGGGATAAGCTGCAAGATATTGCCCTAGGCATAGCCAAAGGAATTGAATATCTTCACCAGGGATGCGATCTACGAATCCTGCATTTCGACATCAAACCCCATAATGTTTTGCTAGACCAAAACTTCACTCCAAAAATTTCTGATTTTGGTTTGGCCAAGTTATGTTCCAAGGATCAAAGCTTGGTGTCCATGACTACAGCTAGGGGGACCATGGGCTACATTGCACCTGAAGTGTTCTCCAGGAATTTTGGAAATGTGTCTTACAAGGCAGATGTCTATAGCTTTGGAATGCTATTGCTTGAGATGGTAGGAGGAAGGAAAAATATTGGTTCAACCACGGAGAACACTACAAATGAAATTTACTATCCACAGTGGATTTATAATCTTCTAGAGGAAGGGGATGACCTACGAATCCATACTGGGGAAGAAGAAGATGGTAAAATTCCAAGGAAACTTGCAATTATAGGGCTATGGTGTATCCAATGGCACCCGTCAGATCGTCCTTCCATGGAAACAGCGGTTCAGATATTAGAAGGAGGAGGAGAAAGCTTGACAGTGCCGCCTAATCCCTTCATGCCTACGGGTCCTACAACTAGAAATGCAAGACGCCTAGAGCTAGAAGCAATTGCTGAATTAGATTTGAGGATGGAAGAAACTTCATGGATACCGGGTAATCAACACAATATTGGGTGGGGTGTAGAGGTGACGCGGAAGGAATTGGCGAGCTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

528

Amino Acids

59.54

Weight (kDa)

5.8

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 190 - 460 1.1e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 191 - 459 3.4e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1233
Acc36I ACCTGC 1 cut(s) 522
AccB1I GGYRCC 2 cut(s) 326, 1338
AccII CGCG 1 cut(s) 1561
AciI CCGC 4 cut(s) 233, 1370, 1412, 1561
AclWI GGATC 1 cut(s) 1032
AcoI YGGCCR 2 cut(s) 241, 1005
AcsI RAATTY 8 cut(s) 28, 129, 632, 838, 987, 1096, 1209, 1293
AcuI CTGAAG 1 cut(s) 1101
AcvI CACGTG 1 cut(s) 355
AcyI GRCGYC 1 cut(s) 1459
AfaI GTAC 1 cut(s) 280
AfiI CCNNNNNNNGG 2 cut(s) 110, 1273
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 9 cut(s) 192, 254, 259, 344, 494, 574, 613, 899, 1185
AjiI CACGTC 1 cut(s) 724
AjnI CCWGG 3 cut(s) 402, 909, 1091
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AluBI AGCT 9 cut(s) 458, 773, 862, 1032, 1052, 1134, 1401, 1468, 1578
AluI AGCT 9 cut(s) 458, 773, 862, 1032, 1052, 1134, 1401, 1468, 1578
Alw21I GWGCWC 1 cut(s) 775
AlwI GGATC 1 cut(s) 1032
AoxI GGCC 2 cut(s) 241, 1005
ApeKI GCWGC 3 cut(s) 123, 437, 862
ApoI RAATTY 8 cut(s) 28, 129, 632, 838, 987, 1096, 1209, 1293
ArsI GACNNNNNNTTYG 2 cut(s) 694, 726
Asp700I GAANNNNTTC 1 cut(s) 903
AspA2I CCTAGG 1 cut(s) 877
AspS9I GGNCC 2 cut(s) 1059, 1436
AsuC2I CCSGG 1 cut(s) 1521
AsuHPI GGTGA 2 cut(s) 899, 1567
AvaII GGWCC 2 cut(s) 1059, 1436
AvrII CCTAGG 1 cut(s) 877
BalI TGGCCA 1 cut(s) 1007
BanI GGYRCC 2 cut(s) 326, 1338
BanII GRGCYC 1 cut(s) 775
BbrPI CACGTG 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 601
Bbv12I GWGCWC 1 cut(s) 775
BbvI GCAGC 3 cut(s) 135, 449, 849
BccI CCATC 6 cut(s) 668, 700, 752, 1150, 1280, 1492
BceAI ACGGC 1 cut(s) 228
BciT130I CCWGG 3 cut(s) 404, 911, 1093
BciVI GTATCC 2 cut(s) 1340, 1509
BcnI CCSGG 1 cut(s) 1521
BfaI CTAG 8 cut(s) 120, 878, 965, 1053, 1241, 1446, 1463, 1469
BfmI CTRYAG 3 cut(s) 806, 1047, 1129
BfuAI ACCTGC 1 cut(s) 522
BfuI GTATCC 2 cut(s) 1340, 1509
BisI GCNGC 4 cut(s) 124, 438, 863, 1412
BlnI CCTAGG 1 cut(s) 877
BlsI GCNGC 4 cut(s) 125, 439, 864, 1413
Bme1390I CCNGG 4 cut(s) 404, 911, 1093, 1521
Bme18I GGWCC 2 cut(s) 1059, 1436
BmgBI CACGTC 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 1059, 1436
BmiI GGNNCC 6 cut(s) 328, 595, 802, 1060, 1340, 1437
BmrFI CCNGG 4 cut(s) 404, 911, 1093, 1521
BmrI ACTGGG 1 cut(s) 1281
BmsI GCATC 1 cut(s) 905
BmuI ACTGGG 1 cut(s) 1281
BpiI GAAGAC 1 cut(s) 601
BpmI CTGGAG 1 cut(s) 1075
BpuEI CTTGAG 1 cut(s) 1172
BpuMI CCSGG 1 cut(s) 1521
BsaAI YACGTR 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 93
BsaHI GRCGYC 1 cut(s) 1459
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 1273
Bse1I ACTGG 1 cut(s) 1276
Bse3DI GCAATG 1 cut(s) 1071
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 3 cut(s) 404, 911, 1093
BseGI GGATG 3 cut(s) 920, 1258, 1503
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 2 cut(s) 110, 1273
BseMI GCAATG 1 cut(s) 1071
BseNI ACTGG 1 cut(s) 1276
BseRI GAGGAG 2 cut(s) 1404, 1407
BseXI GCAGC 3 cut(s) 135, 449, 849
Bsh1236I CGCG 1 cut(s) 1561
BshFI GGCC 2 cut(s) 243, 1007
BshNI GGYRCC 2 cut(s) 326, 1338
BsiHKAI GWGCWC 1 cut(s) 775
BsiSI CCGG 1 cut(s) 1520
BslFI GGGAC 2 cut(s) 86, 1072
BslI CCNNNNNNNGG 2 cut(s) 110, 1273
BsmFI GGGAC 2 cut(s) 86, 1072
BsmI GAATGC 2 cut(s) 624, 1146
BsnI GGCC 2 cut(s) 243, 1007
Bsp1286I GDGCHC 1 cut(s) 775
Bsp1407I TGTACA 1 cut(s) 278
Bsp143I GATC 3 cut(s) 919, 1024, 1348
Bsp19I CCATGG 2 cut(s) 1062, 1359
BspACI CCGC 4 cut(s) 233, 1370, 1412, 1561
BspANI GGCC 2 cut(s) 243, 1007
BspFNI CGCG 1 cut(s) 1561
BspLI GGNNCC 6 cut(s) 328, 595, 802, 1060, 1340, 1437
BspMAI CTGCAG 1 cut(s) 810
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 1 cut(s) 1032
BspT107I GGYRCC 2 cut(s) 326, 1338
BsrDI GCAATG 1 cut(s) 1071
BsrGI TGTACA 1 cut(s) 278
BsrI ACTGG 1 cut(s) 1276
BssMI GATC 3 cut(s) 919, 1024, 1348
BssNI GRCGYC 1 cut(s) 1459
BssT1I CCWWGG 6 cut(s) 666, 877, 1020, 1062, 1298, 1359
Bst2UI CCWGG 3 cut(s) 404, 911, 1093
Bst4CI ACNGT 6 cut(s) 335, 607, 662, 800, 1224, 1408
Bst6I CTCTTC 2 cut(s) 631, 675
BstACI GRCGYC 1 cut(s) 1459
BstAUI TGTACA 1 cut(s) 278
BstBAI YACGTR 1 cut(s) 355
BstC8I GCNNGC 2 cut(s) 736, 1576
BstDSI CCRYGG 3 cut(s) 1062, 1188, 1359
BstF5I GGATG 3 cut(s) 920, 1258, 1503
BstFNI CGCG 1 cut(s) 1561
BstKTI GATC 3 cut(s) 922, 1027, 1351
BstMBI GATC 3 cut(s) 919, 1024, 1348
BstMWI GCNNNNNNNGC 2 cut(s) 120, 366
BstNI CCWGG 3 cut(s) 404, 911, 1093
BstSCI CCNGG 4 cut(s) 402, 909, 1091, 1519
BstSFI CTRYAG 3 cut(s) 806, 1047, 1129
BstUI CGCG 1 cut(s) 1561
BstV1I GCAGC 3 cut(s) 135, 449, 849
BstV2I GAAGAC 1 cut(s) 601
BsuI GTATCC 2 cut(s) 1340, 1509
BsuRI GGCC 2 cut(s) 243, 1007
BtgI CCRYGG 3 cut(s) 1062, 1188, 1359
BtrI CACGTC 1 cut(s) 724
BtsCI GGATG 3 cut(s) 920, 1258, 1503
BtsIMutI CAGTG 3 cut(s) 340, 1229, 1413
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 2 cut(s) 736, 1576
Cfr13I GGNCC 2 cut(s) 1059, 1436
CseI GACGC 2 cut(s) 1467, 1567
Csp6I GTAC 1 cut(s) 279
CviAII CATG 6 cut(s) 375, 1042, 1063, 1360, 1426, 1512
CviQI GTAC 1 cut(s) 279
DpnI GATC 3 cut(s) 921, 1026, 1350
DpnII GATC 3 cut(s) 919, 1024, 1348
EaeI YGGCCR 2 cut(s) 241, 1005
Eam1104I CTCTTC 2 cut(s) 631, 675
EarI CTCTTC 2 cut(s) 631, 675
Ecl136II GAGCTC 1 cut(s) 773
Eco130I CCWWGG 6 cut(s) 666, 877, 1020, 1062, 1298, 1359
Eco24I GRGCYC 1 cut(s) 775
Eco47I GGWCC 2 cut(s) 1059, 1436
Eco53kI GAGCTC 1 cut(s) 773
Eco57I CTGAAG 1 cut(s) 1101
Eco72I CACGTG 1 cut(s) 355
EcoICRI GAGCTC 1 cut(s) 773
EcoO109I RGGNCCY 1 cut(s) 1436
EcoRI GAATTC 2 cut(s) 632, 838
EcoRII CCWGG 3 cut(s) 402, 909, 1091
EcoT14I CCWWGG 6 cut(s) 666, 877, 1020, 1062, 1298, 1359
EcoT38I GRGCYC 1 cut(s) 775
ErhI CCWWGG 6 cut(s) 666, 877, 1020, 1062, 1298, 1359
FaeI CATG 6 cut(s) 378, 1045, 1066, 1363, 1429, 1515
FaqI GGGAC 2 cut(s) 86, 1072
FatI CATG 6 cut(s) 374, 1041, 1062, 1359, 1425, 1511
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 4 cut(s) 124, 438, 863, 1412
FokI GGATG 3 cut(s) 927, 1265, 1510
FriOI GRGCYC 1 cut(s) 775
Fsp4HI GCNGC 4 cut(s) 124, 438, 863, 1412
FspBI CTAG 8 cut(s) 120, 878, 965, 1053, 1241, 1446, 1463, 1469
GluI GCNGC 4 cut(s) 124, 438, 863, 1412
GsuI CTGGAG 1 cut(s) 1075
HaeIII GGCC 2 cut(s) 243, 1007
HapII CCGG 1 cut(s) 1520
HgaI GACGC 2 cut(s) 1467, 1567
Hin1I GRCGYC 1 cut(s) 1459
Hin1II CATG 6 cut(s) 378, 1045, 1066, 1363, 1429, 1515
HincII GTYRAC 2 cut(s) 350, 727
HindII GTYRAC 2 cut(s) 350, 727
HindIII AAGCTT 2 cut(s) 1030, 1399
HinfI GANTC 6 cut(s) 143, 175, 399, 498, 927, 1263
HpaII CCGG 1 cut(s) 1520
HphI GGTGA 2 cut(s) 899, 1567
Hpy166II GTNNAC 3 cut(s) 350, 727, 781
Hpy188I TCNGA 4 cut(s) 631, 994, 1348, 1377
Hpy188III TCNNGA 2 cut(s) 172, 1241
Hpy8I GTNNAC 3 cut(s) 350, 727, 781
HpyAV CCTTC 8 cut(s) 25, 584, 1161, 1241, 1365, 1379, 1432, 1558
HpyCH4III ACNGT 6 cut(s) 335, 607, 662, 800, 1224, 1408
HpyCH4IV ACGT 3 cut(s) 354, 723, 729
HpyCH4V TGCA 9 cut(s) 77, 437, 545, 808, 865, 934, 1076, 1310, 1454
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 366
HpySE526I ACGT 3 cut(s) 354, 723, 729
Hsp92I GRCGYC 1 cut(s) 1459
Hsp92II CATG 6 cut(s) 378, 1045, 1066, 1363, 1429, 1515
Kzo9I GATC 3 cut(s) 919, 1024, 1348
LmnI GCTCC 1 cut(s) 593
Lsp1109I GCAGC 3 cut(s) 135, 449, 849
LweI GCATC 1 cut(s) 905
MaeI CTAG 8 cut(s) 120, 878, 965, 1053, 1241, 1446, 1463, 1469
MaeII ACGT 3 cut(s) 354, 723, 729
MaeIII GTNAC 2 cut(s) 388, 1555
MalI GATC 3 cut(s) 921, 1026, 1350
MboI GATC 3 cut(s) 919, 1024, 1348
MfeI CAATTG 1 cut(s) 1476
MhlI GDGCHC 1 cut(s) 775
MlsI TGGCCA 1 cut(s) 1007
MluNI TGGCCA 1 cut(s) 1007
MlyI GAGTC 1 cut(s) 408
MmeI TCCRAC 1 cut(s) 724
Mox20I TGGCCA 1 cut(s) 1007
MroXI GAANNNNTTC 1 cut(s) 903
MscI TGGCCA 1 cut(s) 1007
MseI TTAA 3 cut(s) 42, 485, 552
Msp20I TGGCCA 1 cut(s) 1007
MspA1I CMGCKG 1 cut(s) 1370
MspI CCGG 1 cut(s) 1520
MspR9I CCNGG 4 cut(s) 404, 911, 1093, 1521
MunI CAATTG 1 cut(s) 1476
Mva1269I GAATGC 2 cut(s) 624, 1146
MvaI CCWGG 3 cut(s) 404, 911, 1093
MvnI CGCG 1 cut(s) 1561
MwoI GCNNNNNNNGC 2 cut(s) 120, 366
NciI CCSGG 1 cut(s) 1521
NcoI CCATGG 2 cut(s) 1062, 1359
NdeII GATC 3 cut(s) 919, 1024, 1348
NlaIII CATG 6 cut(s) 378, 1045, 1066, 1363, 1429, 1515
NlaIV GGNNCC 6 cut(s) 328, 595, 802, 1060, 1340, 1437
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 1555
PctI GAATGC 2 cut(s) 624, 1146
PdmI GAANNNNTTC 1 cut(s) 903
PfeI GAWTC 5 cut(s) 143, 175, 498, 927, 1263
PfoI TCCNGGA 1 cut(s) 1091
PkrI GCNGC 4 cut(s) 125, 439, 864, 1413
PleI GAGTC 1 cut(s) 407
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 1 cut(s) 407
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 1436
PsiI TTATAA 1 cut(s) 1233
Psp124BI GAGCTC 1 cut(s) 775
Psp5II RGGWCCY 1 cut(s) 1436
Psp6I CCWGG 3 cut(s) 402, 909, 1091
PspCI CACGTG 1 cut(s) 355
PspGI CCWGG 3 cut(s) 402, 909, 1091
PspN4I GGNNCC 6 cut(s) 328, 595, 802, 1060, 1340, 1437
PspPI GGNCC 2 cut(s) 1059, 1436
PspPPI RGGWCCY 1 cut(s) 1436
PstI CTGCAG 1 cut(s) 810
RsaI GTAC 1 cut(s) 280
RsaNI GTAC 1 cut(s) 279
SacI GAGCTC 1 cut(s) 775
SaqAI TTAA 3 cut(s) 42, 485, 552
SatI GCNGC 4 cut(s) 124, 438, 863, 1412
Sau3AI GATC 3 cut(s) 919, 1024, 1348
Sau96I GGNCC 2 cut(s) 1059, 1436
SbfI CCTGCAGG 1 cut(s) 810
SchI GAGTC 1 cut(s) 408
ScrFI CCNGG 4 cut(s) 404, 911, 1093, 1521
SdaI CCTGCAGG 1 cut(s) 810
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 1 cut(s) 905
SfcI CTRYAG 3 cut(s) 806, 1047, 1129
SinI GGWCC 2 cut(s) 1059, 1436
SmlI CTYRAG 1 cut(s) 1151
SmoI CTYRAG 1 cut(s) 1151
Sse8387I CCTGCAGG 1 cut(s) 810
SsiI CCGC 4 cut(s) 233, 1370, 1412, 1561
SspI AATATT 2 cut(s) 1177, 1537
SspMI CTAG 8 cut(s) 120, 878, 965, 1053, 1241, 1446, 1463, 1469
SstI GAGCTC 1 cut(s) 775
StyD4I CCNGG 4 cut(s) 402, 909, 1091, 1519
StyI CCWWGG 6 cut(s) 666, 877, 1020, 1062, 1298, 1359
TaaI ACNGT 6 cut(s) 335, 607, 662, 800, 1224, 1408
TaiI ACGT 3 cut(s) 357, 726, 732
TaqI TCGA 2 cut(s) 266, 939
TatI WGTACW 1 cut(s) 278
TauI GCSGC 1 cut(s) 1414
TfiI GAWTC 5 cut(s) 143, 175, 498, 927, 1263
Tru1I TTAA 3 cut(s) 42, 485, 552
Tru9I TTAA 3 cut(s) 42, 485, 552
TscAI CASTG 3 cut(s) 340, 1229, 1413
TseFI GTSAC 1 cut(s) 1555
TseI GCWGC 3 cut(s) 123, 437, 862
Tsp45I GTSAC 1 cut(s) 1555
TspDTI ATGAA 7 cut(s) 708, 805, 810, 831, 1221, 1414, 1500
TspGWI ACGGA 1 cut(s) 1205
TspRI CASTG 3 cut(s) 340, 1229, 1413
VpaK11BI GGWCC 2 cut(s) 1059, 1436
XapI RAATTY 8 cut(s) 28, 129, 632, 838, 987, 1096, 1209, 1293
XbaI TCTAGA 1 cut(s) 1240
XcmI CCANNNNNNNNNTGG 2 cut(s) 225, 372
XmaJI CCTAGG 1 cut(s) 877
XmnI GAANNNNTTC 1 cut(s) 903
XspI CTAG 8 cut(s) 120, 878, 965, 1053, 1241, 1446, 1463, 1469
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.