Rw1G007760

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000107
Physical Location & Seq
Reverse (-)
16449744 .. 16451811
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G007760.1

Sequence Viewer

Length: 1848 bp
ATGCAGCGACTAATCCTCATATCAGCCTATTGCATGCTAATGTCAATGGCTTTCTTCAAGGTGTGCCATAATGCATGCACAGAATCAGAGTGTGGTGACCATGATGATCATCGCCTTGCTATTCATGTCCCGGTCAAGCGCGTCCGTTGTCGTGGTCGTTATTTTCATGAGCAGCAGAGCGACAAGACGATTGTTGAGCAGAAGCAAATGGTAGTAAAATTCTTTGTGAAACACATAGATTATAAGCACCAGAAAATTCAAGTACATATTCCAGATGATTGCTTTCTCCTAAAGCAATTAGAAACCCCAAGCATGAAACCAATGTTCTCTCCTTTGAACTTGTCAGAATATTCCAAATGGTATCCTTGCATCAGCTTATTCAACTGTCCCACAGCTAATTATCCGCACATGCAAGTCCCCTGCCTCAGTGACCTGGTATACAAAAATATCTATGCCGTTTCTTCGGAGGTTGAAGCCCCACAGTACTGTACAAAGATGTATGATTTAATAAATCCATATGGACCTATGCATCTGCCGGGTAACGATTTTTGGTATCCAGATAACTATTTTTTGTATCCAGATTACTATTTTCGGTGGTCTACACCAAATTGTACAGAATGTGAAGCGAAGCGTAAGAAGTGTCAATTGAAGATCAACGGCACCAAAAGTGAAATTGAATGTGTTCATTTTAGCAAACCAAGTGCAACAAAAACATTAGTGTCTACAGGTGCAACTGTGACTACAATCATTTTACTAATATCGGTGACTGTAGTCTATAGATCTCATAAAAAGGAGAAGGAGAATCAATTGAAAATTGAAACATTCCTAGAGGATTACAAAGCGCTCAAGCCAAGCAGATATTCCTACGCAGATATCAAAAGAATTACAAATCAATTTGAGGACAAGTTGGGCCAAGGAGCGTATGGAACTGTTTTCAAAGGGAAGCTTTCATCTGAAGTTTTTGTTGCCGTTAAAGTCCTCAACAATTCCGAAAAAGGAGATGGCGAAGAATTCATCAATGAGGTGGGAACAATGGGTCATATCCACCATGTCAACGTGGTTCGCATGGTTGGCTTCTGTGCAGAAGGATTTAGACGAGCTCTTGTTTATGAGTTTTTCCCCAATGGTTCACTACAGGATTTCATTTCATCAGCAGACAATAAGAATTCTTTCCTTGGTTGGGATAAGTTAAAAGATATTTCTCTAGGCATTGCCAAAGGAATTGAATATCTACACCAAGGATGCGATCAACGAATTCTTCATTTTGATATCAAACCTCAAAATGTTTTGCTAGATCAAAACTTCACTCCAAAAATTTCTGATTTTGGGATGTCCAAGTTGTGTGCCAAGGATCAAAGTATAGTGTCAATGACTATGGCTAGGGGGACTATAGGCTACATTGCACCTGAAGTGTTCTCCCGGAATTTTGGAAATGTGTCATATAAGTCAGATGTCTATAGTTTTGGAATGTTGTTGCTCGAGATTGTAGGAGGGAGGAAGAATATTGGTGAAACCATGGATAACACTGGGGAAGTATACTACCCAGAATGGATACATAATCTTTTAGATGAAGGGGAAGACCTTCGAGTCCATATTGGGGAAGAAGGATCGAGAATAGATTCTCGAATTCCAAAAGAACTAGCAATTGTAGGCCTTTGGTGTATCCAATGGCACCCAATGAATCGTCCATCCATGAAATCAGTGGTTCAGATGTTGGGAGGAAGAGAAAACTTAACCATGCCACCTAATCCTTTTGCCCCTACAGGTCCTACAAGAACAAATGCAACAAGTCGCCCGGCTGCAAGACGCTTCAACCTCGACTTAGAAGCAATTGTTGAATTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase

Protein Analysis

615

Amino Acids

70.36

Weight (kDa)

7.53

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 298 - 572 3.4e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 299 - 570 6.2e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 243
AasI GACNNNNNNGTC 1 cut(s) 1586
AccB1I GGYRCC 2 cut(s) 659, 1671
AccI GTMKAC 4 cut(s) 438, 599, 722, 1536
AccII CGCG 1 cut(s) 141
AciI CCGC 1 cut(s) 404
AclWI GGATC 2 cut(s) 1359, 1615
AcsI RAATTY 8 cut(s) 218, 255, 1010, 1165, 1254, 1314, 1423, 1626
AcuI CTGAAG 2 cut(s) 975, 1428
AfaI GTAC 4 cut(s) 264, 485, 490, 613
AfeI AGCGCT 1 cut(s) 843
AfiI CCNNNNNNNGG 2 cut(s) 1180, 1597
AjnI CCWGG 1 cut(s) 432
AjuI GAANNNNNNNTTGG 2 cut(s) 844, 876
AluBI AGCT 4 cut(s) 375, 395, 946, 1100
AluI AGCT 4 cut(s) 375, 395, 946, 1100
Alw21I GWGCWC 1 cut(s) 1102
AlwI GGATC 2 cut(s) 1359, 1615
Ama87I CYCGRG 1 cut(s) 1478
Aor51HI AGCGCT 1 cut(s) 843
AoxI GGCC 2 cut(s) 910, 1651
ApeKI GCWGC 3 cut(s) 4, 172, 1799
ApoI RAATTY 8 cut(s) 218, 255, 1010, 1165, 1254, 1314, 1423, 1626
Asp700I GAANNNNTTC 4 cut(s) 681, 1169, 1581, 1618
AspLEI GCGC 2 cut(s) 141, 844
AspS9I GGNCC 3 cut(s) 521, 910, 1766
AsuC2I CCSGG 4 cut(s) 131, 537, 1420, 1796
AsuHPI GGTGA 3 cut(s) 107, 775, 1520
AvaI CYCGRG 1 cut(s) 1478
AvaII GGWCC 2 cut(s) 521, 1766
BanI GGYRCC 2 cut(s) 659, 1671
BanII GRGCYC 1 cut(s) 1102
BbsI GAAGAC 1 cut(s) 1584
Bbv12I GWGCWC 1 cut(s) 1102
BbvI GCAGC 3 cut(s) 16, 184, 1786
BccI CCATC 2 cut(s) 995, 1696
BceAI ACGGC 3 cut(s) 440, 673, 953
BciT130I CCWGG 1 cut(s) 434
BciVI GTATCC 5 cut(s) 372, 564, 585, 1545, 1673
BclI TGATCA 1 cut(s) 106
BcnI CCSGG 4 cut(s) 131, 537, 1420, 1796
BfaI CTAG 5 cut(s) 827, 1205, 1292, 1380, 1640
BfmI CTRYAG 7 cut(s) 723, 768, 775, 1133, 1389, 1456, 1761
BfoI RGCGCY 1 cut(s) 845
BfuI GTATCC 5 cut(s) 372, 564, 585, 1545, 1673
BglII AGATCT 1 cut(s) 779
BisI GCNGC 3 cut(s) 5, 173, 1800
BlsI GCNGC 3 cut(s) 6, 174, 1801
BmcAI AGTACT 1 cut(s) 485
Bme1390I CCNGG 5 cut(s) 131, 434, 537, 1420, 1796
Bme18I GGWCC 2 cut(s) 521, 1766
BmeT110I CYCGRG 1 cut(s) 1478
BmgT120I GGNCC 3 cut(s) 521, 910, 1766
BmiI GGNNCC 2 cut(s) 661, 1673
BmrFI CCNGG 5 cut(s) 131, 434, 537, 1420, 1796
BmrI ACTGGG 1 cut(s) 1536
BmsI GCATC 3 cut(s) 378, 538, 1232
BmuI ACTGGG 1 cut(s) 1536
BoxI GACNNNNGTC 1 cut(s) 770
BpiI GAAGAC 1 cut(s) 1584
BpuEI CTTGAG 1 cut(s) 830
BpuMI CCSGG 4 cut(s) 131, 537, 1420, 1796
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 5 cut(s) 913, 1174, 1237, 1347, 1515
Bsc4I CCNNNNNNNGG 2 cut(s) 1180, 1597
Bse1I ACTGG 1 cut(s) 1531
Bse3DI GCAATG 2 cut(s) 1209, 1398
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 5 cut(s) 913, 1174, 1237, 1347, 1515
BseGI GGATG 3 cut(s) 1247, 1335, 1688
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 1180, 1597
BseMI GCAATG 2 cut(s) 1209, 1398
BseMII CTCAG 1 cut(s) 439
BseNI ACTGG 1 cut(s) 1531
BseXI GCAGC 3 cut(s) 16, 184, 1786
BsgI GTGCAG 1 cut(s) 1101
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 2 cut(s) 912, 1653
BshNI GGYRCC 2 cut(s) 659, 1671
BsiHKAI GWGCWC 1 cut(s) 1102
BsiHKCI CYCGRG 1 cut(s) 1478
BsiSI CCGG 4 cut(s) 131, 536, 1420, 1796
BslFI GGGAC 4 cut(s) 113, 372, 401, 1399
BslI CCNNNNNNNGG 2 cut(s) 1180, 1597
BsmFI GGGAC 4 cut(s) 113, 372, 401, 1399
BsnI GGCC 2 cut(s) 912, 1653
BsoBI CYCGRG 1 cut(s) 1478
Bsp1286I GDGCHC 1 cut(s) 1102
Bsp1407I TGTACA 2 cut(s) 488, 611
Bsp143I GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
Bsp19I CCATGG 1 cut(s) 1515
BspACI CCGC 1 cut(s) 404
BspANI GGCC 2 cut(s) 912, 1653
BspCNI CTCAG 1 cut(s) 438
BspFNI CGCG 1 cut(s) 141
BspHI TCATGA 1 cut(s) 166
BspLI GGNNCC 2 cut(s) 661, 1673
BspPI GGATC 2 cut(s) 1359, 1615
BspT107I GGYRCC 2 cut(s) 659, 1671
BsrDI GCAATG 2 cut(s) 1209, 1398
BsrGI TGTACA 2 cut(s) 488, 611
BsrI ACTGG 1 cut(s) 1531
BssECI CCNNGG 5 cut(s) 913, 1174, 1237, 1347, 1515
BssMI GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
BssNAI GTATAC 2 cut(s) 439, 1537
BssT1I CCWWGG 5 cut(s) 913, 1174, 1237, 1347, 1515
Bst1107I GTATAC 2 cut(s) 439, 1537
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 6 cut(s) 386, 483, 488, 736, 769, 931
Bst6I CTCTTC 1 cut(s) 1717
BstAUI TGTACA 2 cut(s) 488, 611
BstC8I GCNNGC 2 cut(s) 35, 76
BstDEI CTNAG 2 cut(s) 425, 1822
BstDSI CCRYGG 1 cut(s) 1515
BstEII GGTNACC 1 cut(s) 95
BstF5I GGATG 3 cut(s) 1247, 1335, 1688
BstFNI CGCG 1 cut(s) 141
BstH2I RGCGCY 1 cut(s) 845
BstHHI GCGC 2 cut(s) 141, 844
BstKTI GATC 7 cut(s) 109, 654, 782, 1249, 1297, 1354, 1610
BstMBI GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
BstMWI GCNNNNNNNGC 1 cut(s) 1071
BstNI CCWGG 1 cut(s) 434
BstNSI RCATGY 3 cut(s) 37, 78, 412
BstPAI GACNNNNGTC 1 cut(s) 770
BstPI GGTNACC 1 cut(s) 95
BstSCI CCNGG 5 cut(s) 129, 432, 535, 1418, 1794
BstSFI CTRYAG 7 cut(s) 723, 768, 775, 1133, 1389, 1456, 1761
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 16, 184, 1786
BstV2I GAAGAC 1 cut(s) 1584
BstX2I RGATCY 1 cut(s) 779
BstYI RGATCY 1 cut(s) 779
BstZ17I GTATAC 2 cut(s) 439, 1537
BsuI GTATCC 5 cut(s) 372, 564, 585, 1545, 1673
BsuRI GGCC 2 cut(s) 912, 1653
BtgI CCRYGG 1 cut(s) 1515
BtgZI GCGATG 1 cut(s) 95
BtsCI GGATG 3 cut(s) 1247, 1335, 1688
BtsIMutI CAGTG 3 cut(s) 433, 1524, 1707
Cac8I GCNNGC 2 cut(s) 35, 76
CciI TCATGA 1 cut(s) 166
CfoI GCGC 2 cut(s) 141, 844
Cfr13I GGNCC 3 cut(s) 521, 910, 1766
CseI GACGC 2 cut(s) 130, 1815
CsiI ACCWGGT 1 cut(s) 432
Csp6I GTAC 4 cut(s) 263, 484, 489, 612
CviQI GTAC 4 cut(s) 263, 484, 489, 612
DdeI CTNAG 2 cut(s) 425, 1822
DpnI GATC 7 cut(s) 108, 653, 781, 1248, 1296, 1353, 1609
DpnII GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
DrdI GACNNNNNNGTC 1 cut(s) 1586
DseDI GACNNNNNNGTC 1 cut(s) 1586
Eam1104I CTCTTC 1 cut(s) 1717
EarI CTCTTC 1 cut(s) 1717
Ecl136II GAGCTC 1 cut(s) 1100
Eco130I CCWWGG 5 cut(s) 913, 1174, 1237, 1347, 1515
Eco147I AGGCCT 1 cut(s) 1653
Eco24I GRGCYC 1 cut(s) 1102
Eco32I GATATC 2 cut(s) 874, 1270
Eco47I GGWCC 2 cut(s) 521, 1766
Eco47III AGCGCT 1 cut(s) 843
Eco53kI GAGCTC 1 cut(s) 1100
Eco57I CTGAAG 2 cut(s) 975, 1428
Eco88I CYCGRG 1 cut(s) 1478
Eco91I GGTNACC 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 1100
EcoO109I RGGNCCY 1 cut(s) 1766
EcoO65I GGTNACC 1 cut(s) 95
EcoRI GAATTC 4 cut(s) 1010, 1165, 1254, 1626
EcoRII CCWGG 1 cut(s) 432
EcoRV GATATC 2 cut(s) 874, 1270
EcoT14I CCWWGG 5 cut(s) 913, 1174, 1237, 1347, 1515
EcoT22I ATGCAT 2 cut(s) 76, 531
EcoT38I GRGCYC 1 cut(s) 1102
ErhI CCWWGG 5 cut(s) 913, 1174, 1237, 1347, 1515
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FaqI GGGAC 4 cut(s) 113, 372, 401, 1399
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 106
FblI GTMKAC 4 cut(s) 438, 599, 722, 1536
Fnu4HI GCNGC 3 cut(s) 5, 173, 1800
FokI GGATG 3 cut(s) 1254, 1342, 1675
FriOI GRGCYC 1 cut(s) 1102
Fsp4HI GCNGC 3 cut(s) 5, 173, 1800
FspBI CTAG 5 cut(s) 827, 1205, 1292, 1380, 1640
GlaI GCGC 2 cut(s) 140, 843
GluI GCNGC 3 cut(s) 5, 173, 1800
HaeII RGCGCY 1 cut(s) 845
HaeIII GGCC 2 cut(s) 912, 1653
HapII CCGG 4 cut(s) 131, 536, 1420, 1796
HgaI GACGC 2 cut(s) 130, 1815
HhaI GCGC 2 cut(s) 141, 844
Hin6I GCGC 2 cut(s) 139, 842
HinP1I GCGC 2 cut(s) 139, 842
HincII GTYRAC 1 cut(s) 1054
HindII GTYRAC 1 cut(s) 1054
HindIII AAGCTT 1 cut(s) 944
HinfI GANTC 5 cut(s) 83, 802, 1587, 1619, 1681
HpaII CCGG 4 cut(s) 131, 536, 1420, 1796
HphI GGTGA 3 cut(s) 107, 775, 1520
Hpy166II GTNNAC 6 cut(s) 439, 600, 723, 1054, 1130, 1537
Hpy188I TCNGA 8 cut(s) 88, 346, 466, 955, 991, 1321, 1450, 1710
Hpy188III TCNNGA 7 cut(s) 167, 272, 557, 578, 1480, 1611, 1623
Hpy8I GTNNAC 6 cut(s) 439, 600, 723, 1054, 1130, 1537
HpyAV CCTTC 5 cut(s) 790, 1079, 1565, 1592, 1598
HpyCH4III ACNGT 6 cut(s) 386, 483, 488, 736, 769, 931
HpyCH4IV ACGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 1 cut(s) 1071
HpyF3I CTNAG 2 cut(s) 425, 1822
HpySE526I ACGT 1 cut(s) 1056
HspAI GCGC 2 cut(s) 139, 842
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
LmnI GCTCC 1 cut(s) 917
Lsp1109I GCAGC 3 cut(s) 16, 184, 1786
LweI GCATC 3 cut(s) 378, 538, 1232
MabI ACCWGGT 1 cut(s) 432
MaeI CTAG 5 cut(s) 827, 1205, 1292, 1380, 1640
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 5 cut(s) 95, 428, 539, 736, 763
MalI GATC 7 cut(s) 108, 653, 781, 1248, 1296, 1353, 1609
MboI GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
MboII GAAGA 9 cut(s) 46, 453, 661, 1019, 1250, 1510, 1589, 1613, 1734
MfeI CAATTG 4 cut(s) 644, 806, 1644, 1830
MflI RGATCY 1 cut(s) 779
MhlI GDGCHC 1 cut(s) 1102
MlyI GAGTC 1 cut(s) 1596
Mph1103I ATGCAT 2 cut(s) 76, 531
MroXI GAANNNNTTC 4 cut(s) 681, 1169, 1581, 1618
MseI TTAA 4 cut(s) 506, 972, 1190, 1733
MslI CAYNNNNRTG 1 cut(s) 38
MspI CCGG 4 cut(s) 131, 536, 1420, 1796
MspR9I CCNGG 5 cut(s) 131, 434, 537, 1420, 1796
MunI CAATTG 4 cut(s) 644, 806, 1644, 1830
MvaI CCWGG 1 cut(s) 434
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 1 cut(s) 1071
NciI CCSGG 4 cut(s) 131, 537, 1420, 1796
NcoI CCATGG 1 cut(s) 1515
NdeI CATATG 1 cut(s) 517
NdeII GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
NlaIV GGNNCC 2 cut(s) 661, 1673
NmuCI GTSAC 4 cut(s) 95, 428, 736, 763
NsiI ATGCAT 2 cut(s) 76, 531
NspI RCATGY 3 cut(s) 37, 78, 412
PaeI GCATGC 2 cut(s) 37, 78
PaeR7I CTCGAG 1 cut(s) 1478
PagI TCATGA 1 cut(s) 166
PceI AGGCCT 1 cut(s) 1653
PdmI GAANNNNTTC 4 cut(s) 681, 1169, 1581, 1618
PfeI GAWTC 4 cut(s) 83, 802, 1619, 1681
PfoI TCCNGGA 1 cut(s) 1418
PkrI GCNGC 3 cut(s) 6, 174, 1801
PleI GAGTC 1 cut(s) 1595
PpsI GAGTC 1 cut(s) 1595
PpuMI RGGWCCY 1 cut(s) 1766
PshAI GACNNNNGTC 1 cut(s) 770
PsiI TTATAA 1 cut(s) 243
Psp124BI GAGCTC 1 cut(s) 1102
Psp5II RGGWCCY 1 cut(s) 1766
Psp6I CCWGG 1 cut(s) 432
PspEI GGTNACC 1 cut(s) 95
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 2 cut(s) 661, 1673
PspPI GGNCC 3 cut(s) 521, 910, 1766
PspPPI RGGWCCY 1 cut(s) 1766
PsuI RGATCY 1 cut(s) 779
RsaI GTAC 4 cut(s) 264, 485, 490, 613
RsaNI GTAC 4 cut(s) 263, 484, 489, 612
RseI CAYNNNNRTG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 1102
SaqAI TTAA 4 cut(s) 506, 972, 1190, 1733
SatI GCNGC 3 cut(s) 5, 173, 1800
Sau3AI GATC 7 cut(s) 106, 651, 779, 1246, 1294, 1351, 1607
Sau96I GGNCC 3 cut(s) 521, 910, 1766
ScaI AGTACT 1 cut(s) 485
SchI GAGTC 1 cut(s) 1596
ScrFI CCNGG 5 cut(s) 131, 434, 537, 1420, 1796
SduI GDGCHC 1 cut(s) 1102
SexAI ACCWGGT 1 cut(s) 432
SfaNI GCATC 3 cut(s) 378, 538, 1232
SfcI CTRYAG 7 cut(s) 723, 768, 775, 1133, 1389, 1456, 1761
Sfr274I CTCGAG 1 cut(s) 1478
SinI GGWCC 2 cut(s) 521, 1766
SlaI CTCGAG 1 cut(s) 1478
SmiMI CAYNNNNRTG 1 cut(s) 38
SmlI CTYRAG 2 cut(s) 845, 1478
SmoI CTYRAG 2 cut(s) 845, 1478
SphI GCATGC 2 cut(s) 37, 78
SseBI AGGCCT 1 cut(s) 1653
SsiI CCGC 1 cut(s) 404
SspI AATATT 2 cut(s) 350, 1504
SspMI CTAG 5 cut(s) 827, 1205, 1292, 1380, 1640
SstI GAGCTC 1 cut(s) 1102
StuI AGGCCT 1 cut(s) 1653
StyD4I CCNGG 5 cut(s) 129, 432, 535, 1418, 1794
StyI CCWWGG 5 cut(s) 913, 1174, 1237, 1347, 1515
TaaI ACNGT 6 cut(s) 386, 483, 488, 736, 769, 931
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 5 cut(s) 1479, 1585, 1610, 1624, 1818
TatI WGTACW 4 cut(s) 262, 483, 488, 611
TfiI GAWTC 4 cut(s) 83, 802, 1619, 1681
Tru1I TTAA 4 cut(s) 506, 972, 1190, 1733
Tru9I TTAA 4 cut(s) 506, 972, 1190, 1733
TscAI CASTG 3 cut(s) 433, 1531, 1707
TseFI GTSAC 4 cut(s) 95, 428, 736, 763
TseI GCWGC 3 cut(s) 4, 172, 1799
Tsp45I GTSAC 4 cut(s) 95, 428, 736, 763
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 3 cut(s) 433, 1531, 1707
VpaK11BI GGWCC 2 cut(s) 521, 1766
XapI RAATTY 8 cut(s) 218, 255, 1010, 1165, 1254, 1314, 1423, 1626
XceI RCATGY 3 cut(s) 37, 78, 412
XcmI CCANNNNNNNNNTGG 2 cut(s) 920, 1699
XhoI CTCGAG 1 cut(s) 1478
XmiI GTMKAC 4 cut(s) 438, 599, 722, 1536
XmnI GAANNNNTTC 4 cut(s) 681, 1169, 1581, 1618
XspI CTAG 5 cut(s) 827, 1205, 1292, 1380, 1640
ZrmI AGTACT 1 cut(s) 485
Zsp2I ATGCAT 2 cut(s) 76, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.