Rw3G022330

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000107
Physical Location & Seq
Reverse (-)
27316851 .. 27319019
2169 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022330.1

Sequence Viewer

Length: 1821 bp
ATGCTTCTCAATTTCCTATGGTGGTTGCTCATAGTAATAGAACTGGTACATGGTGGAGAAGGCCTTGAAAATTGCATCGAAAAGAGGTGTAAACACCATGGCCCAACTATCCGATTTCCCTTTCGAATTAAAGGTAGGCAGCCAAATCACTGTTGTTATGAAGGCTTTGACATATCATGCACCAATGACAAGCAGACTGTTCTGGAGGTCATGCCATTGTTGGAGAGCAAGTTCTTTGTCAAAAACATCAACTATGCATCTCAGGAAATTGAAATATATTACCAGATTGATTGCCCTCCTCAACAGGTTTTTGACCTCATCAGTTTATTGTCTTCTTCCCCATTCAAATTTGTAGAGGGCGGACGGAACTATACATTATTCAGCTGTCCATCATCGAAAGAACTAGACGAATATAGACGATCTCAGGGTCCTTATTGTGTGGCGAAACTGGGGCCTTGCAACCATGGCAGCAATCCTATAGGCAACCAGTTTTACACTGTTCGTGATGATTATTGCAAGGTTGACAGTCTGCCCCTAGTGTCTTGTACTAAGGTGCATGACTACTACACATCATCATCAGTACCAGACATGTCCACAGGAAGCAGGCACAATGTTCTTAAGCTGCAATGGTCCAAGCCATCATGTCAACATTGCGAAGAGATGGGAAAGACATGTAGACTGAAGAGTGAGCAATTCACACCTATCCAGACTGAAACTACTGAATGTTTAGATGTGCCCAAAGGTCATCGTAGCCGTGCAACCCTGAAGATATTAGGTACTTTCTCACCTTCTGTGATTCTCATAGTCACAGGAGCTGTCATTTACTATATCTATAGCTCTAAGAAAACAGAAGATGAAAATCAGCTGAGAATTGAAAGATTTTTGGATGACTACAGAGCTCTCAAGCCAAGTAGATACTCCTATGCTGACATTAAGAGGATAACAAATAAATTTGAGGACAAGTTGGGCCAAGGGGCCTATGGAACAGTGTACAAAGGAAAGCTTTCTTCTGAATTATTTGTTGCTGTGAAAATCCTCAACAATTCAAATGAAAAGGGTGGAGATTTTATAAATGAGGTAGGAACAATGGGTCGAATCCACCATGTCAATGTGGTTTGCTTGGTTGGCTTTTGTGCCGATGGATATATACGAGCTCTCATTTATGAATTCTTACCAAATGGTTCATTGCAGAATTTCTTATCACCAGCAGATCGTAAGAACTCTTTCCTTGGTTGGGATAGGTTGCAAGATATTGCTTTAGGTGTAGCCAAGGGAATTGAATATCTTCACCAAGGGTGTGATCAAAGAATCCTCCATTTTGATATCAAACCCCATAATGTTTTGCTAGAGGAGGACTTCACCCCAAAAGTTTCTGATTTTGGTCTAGCCAAGTTATGTTCTAAGGATCAAAGCGCAATATCCATGACTACAGCCAGAGGGACCATGGGCTACATAGCGCCTGAAGTGTTCTCTAGGAACTTTGGTAACGTGTCCTATAAGTCAGATGTCTATAGTTTTGGAACATTGTTGCTGGAAATGGTTGGGGGTAGAAAAAATTTTAAAGTCACTGAGGACTCCACCAGCGAAGTCTACTTCCCAGAGTGGATCTATAACCTCTTAGAAGAAGGGAACGACCTACGAATCCATATTGAGGACGAAGGAGACAGTAAAATTGCTAGGAAGCTTGCAATCGTGGGTCTATGGTGCATCCAATGGCACCCAGTAGATCGTCCGCCCATGAAACTCGTAGTTCAAATGTTGGAAAGAGAAGGTGACAATCTAACCATGCCTCCTAATCCTTTTGCCTCTACTTCAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

606

Amino Acids

68.83

Weight (kDa)

6.48

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 93 4.7e-15 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 317 - 585 2.5e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 319 - 585 1.7e-45 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1070
AccB1I GGYRCC 1 cut(s) 1716
AccI GTMKAC 2 cut(s) 676, 1590
AciI CCGC 2 cut(s) 360, 1733
AclWI GGATC 2 cut(s) 1413, 1613
AcsI RAATTY 5 cut(s) 347, 950, 1166, 1192, 1555
AcuI CTGAAG 3 cut(s) 701, 785, 1482
AfaI GTAC 5 cut(s) 48, 547, 582, 778, 992
AfiI CCNNNNNNNGG 2 cut(s) 1234, 1651
AflII CTTAAG 1 cut(s) 617
AflIII ACRYGT 3 cut(s) 588, 671, 1488
AgsI TTSAA 8 cut(s) 68, 272, 346, 875, 1047, 1280, 1754, 1815
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 9 cut(s) 384, 622, 815, 837, 865, 899, 1003, 1154, 1684
AluI AGCT 9 cut(s) 384, 622, 815, 837, 865, 899, 1003, 1154, 1684
Alw21I GWGCWC 2 cut(s) 901, 1156
Alw26I GTCTC 1 cut(s) 1656
AlwI GGATC 2 cut(s) 1413, 1613
AlwNI CAGNNNCTG 1 cut(s) 815
AoxI GGCC 5 cut(s) 61, 100, 452, 967, 975
ApeKI GCWGC 3 cut(s) 139, 468, 622
ApoI RAATTY 5 cut(s) 347, 950, 1166, 1192, 1555
Asp700I GAANNNNTTC 3 cut(s) 1003, 1223, 1284
AspLEI GCGC 2 cut(s) 1415, 1459
AspS9I GGNCC 7 cut(s) 101, 428, 452, 630, 967, 975, 1440
AsuHPI GGTGA 5 cut(s) 777, 1194, 1280, 1351, 1784
AsuII TTCGAA 1 cut(s) 124
AvaII GGWCC 3 cut(s) 428, 630, 1440
BaeGI GKGCMC 1 cut(s) 738
BanI GGYRCC 1 cut(s) 1716
BanII GRGCYC 2 cut(s) 901, 1156
BbsI GAAGAC 1 cut(s) 324
Bbv12I GWGCWC 2 cut(s) 901, 1156
BbvI GCAGC 3 cut(s) 151, 480, 609
BccI CCATC 4 cut(s) 397, 646, 655, 1133
BceAI ACGGC 1 cut(s) 738
BclI TGATCA 1 cut(s) 1300
BcoDI GTCTC 1 cut(s) 1656
BfaI CTAG 6 cut(s) 404, 536, 1346, 1385, 1473, 1677
BfmI CTRYAG 5 cut(s) 477, 832, 892, 1428, 1510
BfoI RGCGCY 1 cut(s) 1460
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 3 cut(s) 140, 469, 623
BlsI GCNGC 3 cut(s) 141, 470, 624
Bme18I GGWCC 3 cut(s) 428, 630, 1440
BmgT120I GGNCC 7 cut(s) 101, 428, 452, 630, 967, 975, 1440
BmiI GGNNCC 5 cut(s) 429, 453, 976, 1441, 1718
BmrI ACTGGG 2 cut(s) 458, 1715
BmsI GCATC 3 cut(s) 84, 266, 1716
BmuI ACTGGG 2 cut(s) 458, 1715
BpiI GAAGAC 1 cut(s) 324
BpmI CTGGAG 1 cut(s) 224
Bpu14I TTCGAA 1 cut(s) 124
BpuEI CTTGAG 1 cut(s) 887
BsaBI GATNNNNATC 1 cut(s) 858
BsaJI CCNNGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 1774, 1804
Bsc4I CCNNNNNNNGG 2 cut(s) 1234, 1651
Bse1I ACTGG 4 cut(s) 48, 453, 487, 1721
Bse3DI GCAATG 3 cut(s) 632, 649, 1184
Bse8I GATNNNNATC 1 cut(s) 858
BseDI CCNNGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
BseGI GGATG 2 cut(s) 892, 1707
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 2 cut(s) 1234, 1651
BseMI GCAATG 3 cut(s) 632, 649, 1184
BseMII CTCAG 4 cut(s) 275, 437, 857, 1560
BseNI ACTGG 4 cut(s) 48, 453, 487, 1721
BseRI GAGGAG 2 cut(s) 288, 1364
BseSI GKGCMC 1 cut(s) 738
BseXI GCAGC 3 cut(s) 151, 480, 609
BshFI GGCC 5 cut(s) 63, 102, 454, 969, 977
BshNI GGYRCC 1 cut(s) 1716
BsiHKAI GWGCWC 2 cut(s) 901, 1156
BslFI GGGAC 1 cut(s) 1453
BslI CCNNNNNNNGG 2 cut(s) 1234, 1651
BsmAI GTCTC 1 cut(s) 1656
BsmFI GGGAC 1 cut(s) 1453
BsnI GGCC 5 cut(s) 63, 102, 454, 969, 977
Bsp119I TTCGAA 1 cut(s) 124
Bsp1286I GDGCHC 3 cut(s) 738, 901, 1156
Bsp1407I TGTACA 1 cut(s) 990
Bsp143I GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
Bsp19I CCATGG 3 cut(s) 97, 463, 1443
BspACI CCGC 2 cut(s) 360, 1733
BspANI GGCC 5 cut(s) 63, 102, 454, 969, 977
BspCNI CTCAG 4 cut(s) 274, 436, 858, 1561
BspLI GGNNCC 5 cut(s) 429, 453, 976, 1441, 1718
BspPI GGATC 2 cut(s) 1413, 1613
BspT104I TTCGAA 1 cut(s) 124
BspT107I GGYRCC 1 cut(s) 1716
BspTI CTTAAG 1 cut(s) 617
BsrDI GCAATG 3 cut(s) 632, 649, 1184
BsrGI TGTACA 1 cut(s) 990
BsrI ACTGG 4 cut(s) 48, 453, 487, 1721
BssECI CCNNGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
BssMI GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
BssT1I CCWWGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
Bst4CI ACNGT 6 cut(s) 152, 199, 499, 527, 988, 1667
Bst6I CTCTTC 2 cut(s) 651, 677
BstAFI CTTAAG 1 cut(s) 617
BstAUI TGTACA 1 cut(s) 990
BstBI TTCGAA 1 cut(s) 124
BstC8I GCNNGC 2 cut(s) 605, 1686
BstDEI CTNAG 8 cut(s) 261, 423, 549, 840, 866, 1401, 1569, 1618
BstDSI CCRYGG 3 cut(s) 97, 463, 1443
BstF5I GGATG 2 cut(s) 892, 1707
BstH2I RGCGCY 1 cut(s) 1460
BstHHI GCGC 2 cut(s) 1415, 1459
BstKTI GATC 6 cut(s) 422, 1213, 1303, 1408, 1608, 1729
BstMAI GTCTC 1 cut(s) 1656
BstMBI GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
BstMWI GCNNNNNNNGC 2 cut(s) 465, 1125
BstNSI RCATGY 2 cut(s) 592, 675
BstSFI CTRYAG 5 cut(s) 477, 832, 892, 1428, 1510
BstSLI GKGCMC 1 cut(s) 738
BstV1I GCAGC 3 cut(s) 151, 480, 609
BstV2I GAAGAC 1 cut(s) 324
BstX2I RGATCY 1 cut(s) 1605
BstYI RGATCY 1 cut(s) 1605
BsuRI GGCC 5 cut(s) 63, 102, 454, 969, 977
BtgI CCRYGG 3 cut(s) 97, 463, 1443
BtsCI GGATG 2 cut(s) 892, 1707
BtsIMutI CAGTG 4 cut(s) 148, 495, 993, 1566
Cac8I GCNNGC 2 cut(s) 605, 1686
CaiI CAGNNNCTG 1 cut(s) 815
CfoI GCGC 2 cut(s) 1415, 1459
Cfr13I GGNCC 7 cut(s) 101, 428, 452, 630, 967, 975, 1440
Csp6I GTAC 5 cut(s) 47, 546, 581, 777, 991
CviQI GTAC 5 cut(s) 47, 546, 581, 777, 991
DdeI CTNAG 8 cut(s) 261, 423, 549, 840, 866, 1401, 1569, 1618
DpnI GATC 6 cut(s) 421, 1212, 1302, 1407, 1607, 1728
DpnII GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
DraI TTTAAA 1 cut(s) 1561
Eam1104I CTCTTC 2 cut(s) 651, 677
EarI CTCTTC 2 cut(s) 651, 677
EciI GGCGGA 2 cut(s) 375, 1722
Ecl136II GAGCTC 2 cut(s) 899, 1154
Eco130I CCWWGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
Eco147I AGGCCT 1 cut(s) 63
Eco24I GRGCYC 2 cut(s) 901, 1156
Eco32I GATATC 1 cut(s) 1324
Eco47I GGWCC 3 cut(s) 428, 630, 1440
Eco53kI GAGCTC 2 cut(s) 899, 1154
Eco57I CTGAAG 3 cut(s) 701, 785, 1482
EcoICRI GAGCTC 2 cut(s) 899, 1154
EcoO109I RGGNCCY 3 cut(s) 428, 452, 975
EcoRI GAATTC 1 cut(s) 1166
EcoRV GATATC 1 cut(s) 1324
EcoT14I CCWWGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
EcoT22I ATGCAT 1 cut(s) 259
EcoT38I GRGCYC 2 cut(s) 901, 1156
ErhI CCWWGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
FalI AAGNNNNNCTT 2 cut(s) 987, 1019
FaqI GGGAC 1 cut(s) 1453
FbaI TGATCA 1 cut(s) 1300
FblI GTMKAC 2 cut(s) 676, 1590
Fnu4HI GCNGC 3 cut(s) 140, 469, 623
FokI GGATG 2 cut(s) 899, 1694
FriOI GRGCYC 2 cut(s) 901, 1156
Fsp4HI GCNGC 3 cut(s) 140, 469, 623
FspBI CTAG 6 cut(s) 404, 536, 1346, 1385, 1473, 1677
GlaI GCGC 2 cut(s) 1414, 1458
GluI GCNGC 3 cut(s) 140, 469, 623
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 1460
HaeIII GGCC 5 cut(s) 63, 102, 454, 969, 977
HhaI GCGC 2 cut(s) 1415, 1459
Hin6I GCGC 2 cut(s) 1413, 1457
HinP1I GCGC 2 cut(s) 1413, 1457
HincII GTYRAC 2 cut(s) 523, 647
HindII GTYRAC 2 cut(s) 523, 647
HindIII AAGCTT 2 cut(s) 1001, 1682
HinfI GANTC 5 cut(s) 796, 1095, 1308, 1574, 1641
HphI GGTGA 5 cut(s) 777, 1194, 1280, 1351, 1784
Hpy166II GTNNAC 7 cut(s) 92, 523, 594, 647, 677, 991, 1591
Hpy188I TCNGA 4 cut(s) 113, 1012, 1375, 1504
Hpy188III TCNNGA 4 cut(s) 203, 263, 503, 706
Hpy8I GTNNAC 7 cut(s) 92, 523, 594, 647, 677, 991, 1591
HpyAV CCTTC 6 cut(s) 53, 155, 798, 1619, 1652, 1763
HpyCH4III ACNGT 6 cut(s) 152, 199, 499, 527, 988, 1667
HpyCH4IV ACGT 1 cut(s) 1488
HpyF10VI GCNNNNNNNGC 2 cut(s) 465, 1125
HpyF3I CTNAG 8 cut(s) 261, 423, 549, 840, 866, 1401, 1569, 1618
HpySE526I ACGT 1 cut(s) 1488
HspAI GCGC 2 cut(s) 1413, 1457
Ksp22I TGATCA 1 cut(s) 1300
Kzo9I GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
LmnI GCTCC 1 cut(s) 812
Lsp1109I GCAGC 3 cut(s) 151, 480, 609
LweI GCATC 3 cut(s) 84, 266, 1716
MaeI CTAG 6 cut(s) 404, 536, 1346, 1385, 1473, 1677
MaeII ACGT 1 cut(s) 1488
MaeIII GTNAC 4 cut(s) 805, 1484, 1564, 1772
MalI GATC 6 cut(s) 421, 1212, 1302, 1407, 1607, 1728
MboI GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
MboII GAAGA 9 cut(s) 324, 327, 668, 694, 778, 863, 999, 1277, 1634
MflI RGATCY 1 cut(s) 1605
MhlI GDGCHC 3 cut(s) 738, 901, 1156
MlyI GAGTC 1 cut(s) 1568
MmeI TCCRAC 2 cut(s) 201, 1740
Mph1103I ATGCAT 1 cut(s) 259
MroXI GAANNNNTTC 3 cut(s) 1003, 1223, 1284
MseI TTAA 4 cut(s) 129, 618, 933, 1560
MslI CAYNNNNRTG 1 cut(s) 1107
MspA1I CMGCKG 2 cut(s) 384, 865
MspCI CTTAAG 1 cut(s) 617
MwoI GCNNNNNNNGC 2 cut(s) 465, 1125
NcoI CCATGG 3 cut(s) 97, 463, 1443
NdeII GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
NlaIV GGNNCC 5 cut(s) 429, 453, 976, 1441, 1718
NmuCI GTSAC 3 cut(s) 805, 1564, 1772
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 2 cut(s) 592, 675
NspV TTCGAA 1 cut(s) 124
PceI AGGCCT 1 cut(s) 63
PciI ACATGT 2 cut(s) 588, 671
PdmI GAANNNNTTC 3 cut(s) 1003, 1223, 1284
PfeI GAWTC 4 cut(s) 796, 1095, 1308, 1641
PkrI GCNGC 3 cut(s) 141, 470, 624
PleI GAGTC 1 cut(s) 1568
PpsI GAGTC 1 cut(s) 1568
PpuMI RGGWCCY 1 cut(s) 428
PscI ACATGT 2 cut(s) 588, 671
PsiI TTATAA 1 cut(s) 1070
Psp124BI GAGCTC 2 cut(s) 901, 1156
Psp5II RGGWCCY 1 cut(s) 428
PspN4I GGNNCC 5 cut(s) 429, 453, 976, 1441, 1718
PspPI GGNCC 7 cut(s) 101, 428, 452, 630, 967, 975, 1440
PspPPI RGGWCCY 1 cut(s) 428
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 1 cut(s) 1605
PvuII CAGCTG 2 cut(s) 384, 865
RsaI GTAC 5 cut(s) 48, 547, 582, 778, 992
RsaNI GTAC 5 cut(s) 47, 546, 581, 777, 991
RseI CAYNNNNRTG 1 cut(s) 1107
SacI GAGCTC 2 cut(s) 901, 1156
SaqAI TTAA 4 cut(s) 129, 618, 933, 1560
SatI GCNGC 3 cut(s) 140, 469, 623
Sau3AI GATC 6 cut(s) 419, 1210, 1300, 1405, 1605, 1726
Sau96I GGNCC 7 cut(s) 101, 428, 452, 630, 967, 975, 1440
SchI GAGTC 1 cut(s) 1568
SduI GDGCHC 3 cut(s) 738, 901, 1156
SfaNI GCATC 3 cut(s) 84, 266, 1716
SfcI CTRYAG 5 cut(s) 477, 832, 892, 1428, 1510
SfuI TTCGAA 1 cut(s) 124
SinI GGWCC 3 cut(s) 428, 630, 1440
SmiMI CAYNNNNRTG 1 cut(s) 1107
SmlI CTYRAG 2 cut(s) 617, 902
SmoI CTYRAG 2 cut(s) 617, 902
SseBI AGGCCT 1 cut(s) 63
SsiI CCGC 2 cut(s) 360, 1733
SspMI CTAG 6 cut(s) 404, 536, 1346, 1385, 1473, 1677
SstI GAGCTC 2 cut(s) 901, 1156
StuI AGGCCT 1 cut(s) 63
StyI CCWWGG 7 cut(s) 97, 463, 970, 1228, 1269, 1291, 1443
TaaI ACNGT 6 cut(s) 152, 199, 499, 527, 988, 1667
TaiI ACGT 1 cut(s) 1491
TaqI TCGA 4 cut(s) 78, 124, 395, 1093
TatI WGTACW 2 cut(s) 545, 990
TfiI GAWTC 4 cut(s) 796, 1095, 1308, 1641
Tru1I TTAA 4 cut(s) 129, 618, 933, 1560
Tru9I TTAA 4 cut(s) 129, 618, 933, 1560
TscAI CASTG 4 cut(s) 155, 502, 993, 1573
TseFI GTSAC 3 cut(s) 805, 1564, 1772
TseI GCWGC 3 cut(s) 139, 468, 622
Tsp45I GTSAC 3 cut(s) 805, 1564, 1772
TspDTI ATGAA 6 cut(s) 174, 870, 1065, 1173, 1179, 1754
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 4 cut(s) 155, 502, 993, 1573
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 3 cut(s) 428, 630, 1440
XapI RAATTY 5 cut(s) 347, 950, 1166, 1192, 1555
XceI RCATGY 2 cut(s) 592, 675
XcmI CCANNNNNNNNNTGG 2 cut(s) 977, 1441
XmiI GTMKAC 2 cut(s) 676, 1590
XmnI GAANNNNTTC 3 cut(s) 1003, 1223, 1284
XspI CTAG 6 cut(s) 404, 536, 1346, 1385, 1473, 1677
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.