MD02G1274600.v1.1

receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000107
Physical Location & Seq
Forward (+)
32912603 .. 32914929
2327 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1274600.v1.1.491

Sequence Viewer

Length: 1842 bp
ATGAGAAATTCTGGTGTAAACTCAAACATGACAATCTCTTTCTTCATCCTGTTGTTCACGGTAGGTCTCATTATTAACCTTGGAGCATGCCAAAATGAGTGCACAGAATCACACTGCGGAGACTATGGCCCGCCTATCCGATTTCCATTTCGACTCAAAGACAAACAACCAAGCCACTGTGGCTATCCTGGATTTGATTTATCCTGCGATCGAAGCAACAACACGGTGCTTGAGCTGCCAGTTTCCATTACGCTCTTTGTCACTAGTATTAACTACAAATCTCAGGTGATTCAAGTATTTGATCCAGATCATTGTGTTCCTAGACACCTTCCGAAACTTAATTTATCTTCCTCTGCTTTCATATTCCAAGAGCCGTACCAGTGTGACTATACCTTATTTAATTGTTCACCAGCAAAAAGAGATCTATATTATGATTCAATCCCTTGCCTTAGTGGGACTAGCCATCACATATTTTCTATACCTTCAGATAGTTTCGTTGACAGTCTTCCCCTTACATCTTGTACCAAGTTGTACAACCTTCCATTCAGTGTTCCATATGATATATTCGAACCAAATTATTTTCTTCGTTTGGAATGGTCCAAACCAACATGTAACCGATGTGAAAGGAGAGGTAAAGGATGTAGACTGAAGAACAACAATCGCACCAACTCCGAAACTGAATGCTTCAATGTAAAGCGAAACGGTACTGTGAAAAAACTAGTTGTTACAGGTGCAGTCCTTGGTTCATTTTCACTTGTGCTGATACTTGTCGGACTCCATCGTGTATATACTTTTGACCGAAAAGAAAAGGAGCGGCAAACCATGATCGAAGAATTTTTGGAAAACTACAGAGCTCTCAGGCCTACAAGGTACTCATATGCAGATATCAAGCGGATTACAAATAAATTTAGAGACAAGTTGGGACAGGGAACATTTGGAACAGTGTTCAAAGGAAAGCTTTCGAGTGAGATCTTTGTTGCTGTAAAGATGCTCAACAATTCCAAAGGAAATGGAGGAGAGTTTATCAATGAAGTGGGAACATTAGGTCGGATCCACCATGTTAACGTGGTTCGCTTGGTTGGATATTGCGCTGATGGATTTAGGCGAGCCTTAGTTTATGAATTTGCACCAAACGGTTCGCTGCAAAATTTCATATCGTCAGCAGACAGCAAGAACCATTTCCTTGGTTGGGAGAAGCTGCAACATATTACTATCAGAATAGCCAAGGGAATTGAGTATCTTCACCACGGATGTGACCAACGAATCCTTCATTTCGATGTCAAACCTCACAATGTTCTATTAGATGACCACTTCAACCCAAAAATCTCGGATTTTGGTCTTGCCAAGTTGTGCTCCAAGGACCAAAGCGCAGTTTCCATGACAACTGCTAGGGGTACCATGGGATACATTGCACCGGAAGTGTTCTCAAGGAACTTTGGTACCGTGTCTTACAAGTCGGATGTTTACAGTTTCGGAATGTTGTTACTTGAAATGGTAGGGGGCAAGAAAAATGTTCAAGTCACAGAGAAGAATACCGGGCAAGTTTATTTCCCCGAATGGATCTATAATCTTCTGGAACAAGGGGACGACATACGCATCCATGTTGAGGGAGAGAATGATGCTAAAATTGCCACAAAACTTGCAATTGTGGGGCTATGGTGCATCCAGTGGTATCCAGTAGACCGTCCTTCAATGAAAGCTGTCGTTCAAATGTTGGAAGGAGAAGATAACGTAAAATTGCCTCCTAATCCTTTCTCCCCCGCTGGTTCAGTAAGGAACGGTATCACTATGCTTGCAAGACGCCATCGCCAAGAGTTACCAGTCATCTCCGAATTAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

614

Amino Acids

69.46

Weight (kDa)

8.71

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 34 - 101 9.3e-19 Wall-associated receptor kinase galacturonan-binding
PK_Tyr_Ser-Thr PF07714 303 - 572 8e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 305 - 570 1.5e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000107)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03331 FvH4_1g03331 FvH4_4g22040 FvH4_4g34420 FvH4_4g34420 FvH4_4g34420 FvH4_6g23840 FvH4_6g28420 FvH4_6g52680 FvH4_7g06000 FvH4_7g06000 FvH4_7g06020 FvH4_7g06020 FvH4_7g06020 FvH4_7g06040
malus_domestica MD01G1059600.v1.1 MD02G1234300.v1.1 MD02G1234800.v1.1 MD02G1235400.v1.1 MD02G1245600.v1.1 MD02G1246100.v1.1 MD02G1246600.v1.1 MD02G1247200.v1.1 MD02G1247600.v1.1 MD02G1249400.v1.1 MD02G1249800.v1.1 MD02G1250400.v1.1 MD02G1251000.v1.1 MD02G1252000.v1.1 MD02G1252900.v1.1 MD02G1253800.v1.1 MD02G1254300.v1.1 MD02G1273700.v1.1 MD02G1274600.v1.1 MD07G1070200.v1.1 MD07G1070500.v1.1 MD07G1070800.v1.1 MD07G1071300.v1.1 MD07G1138100.v1.1 MD12G1080000.v1.1 MD12G1251300.v1.1 MD12G1251700.v1.1
prunus_persica Prupe.2G047400_v2.0.a1 Prupe.2G047400_v2.0.a1 Prupe.2G067000_v2.0.a1 Prupe.2G087200_v2.0.a1 Prupe.2G087300_v2.0.a1 Prupe.2G087600_v2.0.a1 Prupe.2G087900_v2.0.a1 Prupe.2G088200_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088400_v2.0.a1 Prupe.2G088900_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089000_v2.0.a1 Prupe.2G089100_v2.0.a1 Prupe.2G103400_v2.0.a1 Prupe.2G104000_v2.0.a1 Prupe.2G312100_v2.0.a1 Prupe.6G137900_v2.0.a1 Prupe.6G142200_v2.0.a1
pyrus_communis pycom01g01220 pycom01g08850 pycom01g08880 pycom01g08910 pycom01g08950 pycom02g20220 pycom02g21130 pycom02g21370 pycom02g21390 pycom02g21410 pycom02g21530 pycom02g21570 pycom02g23470 pycom02g23500 pycom07g05390
rosa_chinensis RchiOBHm_Chr1g0324371 RchiOBHm_Chr1g0324431 RchiOBHm_Chr1g0325121 RchiOBHm_Chr1g0328351 RchiOBHm_Chr1g0328391 RchiOBHm_Chr1g0329541 RchiOBHm_Chr1g0333301 RchiOBHm_Chr1g0333311 RchiOBHm_Chr1g0333361 RchiOBHm_Chr1g0333431 RchiOBHm_Chr1g0333461 RchiOBHm_Chr1g0333471 RchiOBHm_Chr1g0333541 RchiOBHm_Chr1g0333621 RchiOBHm_Chr1g0333631 RchiOBHm_Chr1g0334291 RchiOBHm_Chr1g0334781 RchiOBHm_Chr1g0334821 RchiOBHm_Chr3g0482471 RchiOBHm_Chr5g0047431 RchiOBHm_Chr5g0047561 RchiOBHm_Chr5g0047571 RchiOBHm_Chr5g0047611 RchiOBHm_Chr5g0047651 RchiOBHm_Chr5g0047751 RchiOBHm_Chr5g0047821 RchiOBHm_Chr5g0047961 RchiOBHm_Chr5g0048061 RchiOBHm_Chr6g0283301
rosa_laevigata RLG00000019267 RLG00000023349 RLG00000029423 RLG00000029545 RLG00000029554 RLG00000029607 RLG00000029610 RLG00000029611 RLG00000029614 RLG00000029616 RLG00000029931 RLG00000029932 RLG00000034493
rosa_multiflora Rmu_co8235835.1_g000001 Rmu_sc0000148.1_g000011 Rmu_sc0000148.1_g000032 Rmu_sc0000148.1_g000074 Rmu_sc0000215.1_g000031 Rmu_sc0000494.1_g000013 Rmu_sc0000574.1_g000036 Rmu_sc0000580.1_g000030 Rmu_sc0000580.1_g000031 Rmu_sc0000725.1_g000007 Rmu_sc0000725.1_g000008 Rmu_sc0000725.1_g000009 Rmu_sc0000982.1_g000049 Rmu_sc0001009.1_g000037 Rmu_sc0002955.1_g000008 Rmu_sc0002955.1_g000024 Rmu_sc0002955.1_g000028 Rmu_sc0002965.1_g000023 Rmu_sc0002965.1_g000024 Rmu_sc0002969.1_g000008 Rmu_sc0003435.1_g000013 Rmu_sc0003441.1_g000002 Rmu_sc0003441.1_g000012 Rmu_sc0003441.1_g000034 Rmu_sc0004646.1_g000044 Rmu_sc0004736.1_g000015 Rmu_sc0005613.1_g000001 Rmu_sc0006031.1_g000009 Rmu_sc0007681.1_g000010 Rmu_sc0013030.1_g000001 Rmu_sc0013038.1_g000011 Rmu_sc0016170.1_g000008 Rmu_sc0019659.1_g000001 Rmu_sc0021068.1_g000002 Rmu_ssc0000388.1_g000040
rosa_roxburghii Rroxscaffold_159G00432810 Rroxscaffold_1G00033860 Rroxscaffold_1G00033950 Rroxscaffold_1G00033960 Rroxscaffold_4G00316500 Rroxscaffold_4G00316570 Rroxscaffold_4G00317370 Rroxscaffold_4G00317440 Rroxscaffold_4G00317510 Rroxscaffold_4G00317530 Rroxscaffold_4G00317540 Rroxscaffold_4G00317620 Rroxscaffold_4G00317630 Rroxscaffold_4G00320890 Rroxscaffold_4G00321680 Rroxscaffold_4G00324900 Rroxscaffold_6G00399330
rosa_rugosa Rorug01G0074700 Rorug01G0081200 Rorug01G0103300 Rorug01G0108500 Rorug01G0108700 Rorug01G0108800 Rorug01G0109100 Rorug01G0109200 Rorug01G0109200 Rorug01G0109800 Rorug01G0109800 Rorug01G0110500 Rorug01G0116300 Rorug01G0116800 Rorug02G0305500 Rorug02G0305600 Rorug03G0071700 Rorug05G0163600 Rorug05G0236200 Rorug06G0030200
rosa_samantha Rh1AG092400 Rh1AG099600 Rh1AG131700 Rh1AG131900 Rh1AG132100 Rh1AG132900 Rh1AG140800 Rh1BG028200 Rh1BG074500 Rh1BG079300 Rh1BG101100 Rh1CG067200 Rh1CG125300 Rh1CG125600 Rh1CG125700 Rh1CG126000 Rh1CG127100 Rh1CG127200 Rh1CG132700 Rh1DG144900 Rh2CG455700 Rh3BG018200 Rh3DG018400 Rh3DG275900 Rh4AG065500 Rh4BG330300 Rh4CG345700 Rh5AG315100 Rh5AG315500 Rh5BG324300 Rh5BG324700 Rh5CG351000 Rh5CG351200 Rh5CG351600 Rh5CG351800 Rh5CG352100 Rh6AG265600 Rh6CG267700 Rh6DG261200
rosa_wichuraiana Rw0G001010 Rw0G003190 Rw0G013160 Rw0G022840 Rw1G007100 Rw1G007220 Rw1G007760 Rw1G010730 Rw1G010790 Rw1G010870 Rw1G010910 Rw1G010940 Rw1G011000 Rw1G011010 Rw1G011040 Rw1G011620 Rw2G029190 Rw3G022330 Rw5G029440 Rw5G029470 Rw5G029560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1394, 1439
AccB1I GGYRCC 2 cut(s) 1394, 1439
AccBSI CCGCTC 1 cut(s) 814
AccI GTMKAC 2 cut(s) 643, 1680
AciI CCGC 5 cut(s) 117, 131, 814, 892, 1761
AclWI GGATC 4 cut(s) 296, 1045, 1058, 1568
AcsI RAATTY 5 cut(s) 7, 833, 905, 1121, 1147
AcuI CTGAAG 2 cut(s) 468, 668
AcyI GRCGYC 1 cut(s) 1801
AfaI GTAC 7 cut(s) 377, 523, 533, 706, 872, 1396, 1441
AfiI CCNNNNNNNGG 2 cut(s) 1189, 1606
AflIII ACRYGT 1 cut(s) 608
AgsI TTSAA 9 cut(s) 293, 438, 688, 949, 1315, 1490, 1517, 1692, 1709
AhlI ACTAGT 2 cut(s) 263, 718
AjnI CCWGG 1 cut(s) 187
AjuI GAANNNNNNNTTGG 2 cut(s) 1251, 1283
AleI CACNNNNGTG 1 cut(s) 1251
AluBI AGCT 5 cut(s) 235, 854, 958, 1198, 1700
AluI AGCT 5 cut(s) 235, 854, 958, 1198, 1700
Alw21I GWGCWC 3 cut(s) 104, 856, 1355
Alw26I GTCTC 3 cut(s) 71, 114, 906
Alw44I GTGCAC 1 cut(s) 100
AlwI GGATC 4 cut(s) 296, 1045, 1058, 1568
AoxI GGCC 2 cut(s) 127, 860
ApaLI GTGCAC 1 cut(s) 100
ApeKI GCWGC 3 cut(s) 235, 1141, 1198
ApoI RAATTY 5 cut(s) 7, 833, 905, 1121, 1147
Asp700I GAANNNNTTC 2 cut(s) 958, 1178
Asp718I GGTACC 2 cut(s) 1394, 1439
AspLEI GCGC 2 cut(s) 1091, 1370
AspS9I GGNCC 3 cut(s) 128, 597, 1360
AsuC2I CCSGG 1 cut(s) 1537
AsuHPI GGTGA 3 cut(s) 298, 399, 1235
AsuII TTCGAA 1 cut(s) 567
AvaII GGWCC 2 cut(s) 597, 1360
BaeGI GKGCMC 1 cut(s) 104
BaeI ACNNNNGTAYC 2 cut(s) 1423, 1456
BamHI GGATCC 1 cut(s) 1050
BanI GGYRCC 2 cut(s) 1394, 1439
BanII GRGCYC 1 cut(s) 856
BbsI GAAGAC 1 cut(s) 497
Bbv12I GWGCWC 3 cut(s) 104, 856, 1355
BbvI GCAGC 3 cut(s) 222, 1128, 1185
BccI CCATC 4 cut(s) 471, 786, 1088, 1812
BceAI ACGGC 1 cut(s) 358
BciT130I CCWGG 1 cut(s) 189
BciVI GTATCC 2 cut(s) 1397, 1683
BcnI CCSGG 1 cut(s) 1537
BcoDI GTCTC 3 cut(s) 71, 114, 906
BcuI ACTAGT 2 cut(s) 263, 718
BfaI CTAG 5 cut(s) 264, 321, 459, 719, 1389
BfmI CTRYAG 1 cut(s) 847
BfuI GTATCC 2 cut(s) 1397, 1683
BglI GCCNNNNNGGC 1 cut(s) 180
BglII AGATCT 2 cut(s) 421, 969
BisI GCNGC 4 cut(s) 236, 815, 1142, 1199
BlsI GCNGC 4 cut(s) 237, 816, 1143, 1200
Bme1390I CCNGG 2 cut(s) 189, 1537
Bme18I GGWCC 2 cut(s) 597, 1360
BmgT120I GGNCC 3 cut(s) 128, 597, 1360
BmiI GGNNCC 3 cut(s) 1052, 1396, 1441
BmrFI CCNGG 2 cut(s) 189, 1537
BmsI GCATC 4 cut(s) 978, 1605, 1609, 1671
BpiI GAAGAC 1 cut(s) 497
Bpu14I TTCGAA 1 cut(s) 567
BpuEI CTTGAG 2 cut(s) 251, 1411
BpuMI CCSGG 1 cut(s) 1537
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 1801
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 7 cut(s) 79, 739, 1183, 1224, 1246, 1356, 1398
BsaWI WCCGGW 1 cut(s) 1414
Bsc4I CCNNNNNNNGG 2 cut(s) 1189, 1606
Bse1I ACTGG 5 cut(s) 239, 379, 1666, 1676, 1820
Bse3DI GCAATG 1 cut(s) 1407
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 7 cut(s) 79, 739, 1183, 1224, 1246, 1356, 1398
BseGI GGATG 6 cut(s) 45, 644, 1256, 1465, 1596, 1662
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 1189, 1606
BseMI GCAATG 1 cut(s) 1407
BseMII CTCAG 2 cut(s) 296, 871
BseNI ACTGG 5 cut(s) 239, 379, 1666, 1676, 1820
BseRI GAGGAG 1 cut(s) 1029
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 3 cut(s) 222, 1128, 1185
BsgI GTGCAG 1 cut(s) 753
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 2 cut(s) 129, 862
BshNI GGYRCC 2 cut(s) 1394, 1439
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 3 cut(s) 104, 856, 1355
BsiSI CCGG 2 cut(s) 1415, 1536
BslFI GGGAC 3 cut(s) 469, 936, 1598
BslI CCNNNNNNNGG 2 cut(s) 1189, 1606
BsmAI GTCTC 3 cut(s) 71, 114, 906
BsmFI GGGAC 3 cut(s) 469, 936, 1598
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 2 cut(s) 129, 862
Bso31I GGTCTC 1 cut(s) 71
Bsp119I TTCGAA 1 cut(s) 567
Bsp1286I GDGCHC 3 cut(s) 104, 856, 1355
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
Bsp19I CCATGG 1 cut(s) 1398
BspACI CCGC 5 cut(s) 117, 131, 814, 892, 1761
BspANI GGCC 2 cut(s) 129, 862
BspCNI CTCAG 2 cut(s) 295, 870
BspLI GGNNCC 3 cut(s) 1052, 1396, 1441
BspPI GGATC 4 cut(s) 296, 1045, 1058, 1568
BspT104I TTCGAA 1 cut(s) 567
BspT107I GGYRCC 2 cut(s) 1394, 1439
BspTNI GGTCTC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 814
BsrDI GCAATG 1 cut(s) 1407
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 5 cut(s) 239, 379, 1666, 1676, 1820
BssECI CCNNGG 7 cut(s) 79, 739, 1183, 1224, 1246, 1356, 1398
BssMI GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
BssNI GRCGYC 1 cut(s) 1801
BssT1I CCWWGG 6 cut(s) 79, 739, 1183, 1224, 1356, 1398
Bst2UI CCWGG 1 cut(s) 189
BstACI GRCGYC 1 cut(s) 1801
BstAUI TGTACA 1 cut(s) 531
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 4 cut(s) 88, 131, 1107, 1794
BstDEI CTNAG 4 cut(s) 282, 449, 857, 1111
BstDSI CCRYGG 2 cut(s) 1246, 1398
BstF5I GGATG 6 cut(s) 45, 644, 1256, 1465, 1596, 1662
BstHHI GCGC 2 cut(s) 1091, 1370
BstKTI GATC 8 cut(s) 211, 304, 310, 424, 828, 972, 1053, 1563
BstMAI GTCTC 3 cut(s) 71, 114, 906
BstMBI GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 4 cut(s) 180, 213, 235, 1628
BstNI CCWGG 1 cut(s) 189
BstNSI RCATGY 2 cut(s) 90, 612
BstSCI CCNGG 2 cut(s) 187, 1535
BstSFI CTRYAG 1 cut(s) 847
BstSLI GKGCMC 1 cut(s) 104
BstV1I GCAGC 3 cut(s) 222, 1128, 1185
BstV2I GAAGAC 1 cut(s) 497
BstX2I RGATCY 4 cut(s) 421, 969, 1050, 1560
BstXI CCANNNNNNTGG 1 cut(s) 1184
BstYI RGATCY 4 cut(s) 421, 969, 1050, 1560
BsuI GTATCC 2 cut(s) 1397, 1683
BsuRI GGCC 2 cut(s) 129, 862
BtgI CCRYGG 2 cut(s) 1246, 1398
BtgZI GCGATG 1 cut(s) 1790
BtsCI GGATG 6 cut(s) 45, 644, 1256, 1465, 1596, 1662
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 6 cut(s) 112, 175, 386, 553, 948, 1673
Cac8I GCNNGC 4 cut(s) 88, 131, 1107, 1794
CfoI GCGC 2 cut(s) 1091, 1370
Cfr13I GGNCC 3 cut(s) 128, 597, 1360
CseI GACGC 1 cut(s) 1809
Csp6I GTAC 7 cut(s) 376, 522, 532, 705, 871, 1395, 1440
CspCI CAANNNNNGTGG 2 cut(s) 1621, 1656
CviAII CATG 8 cut(s) 28, 87, 609, 823, 1058, 1378, 1399, 1601
CviQI GTAC 7 cut(s) 376, 522, 532, 705, 871, 1395, 1440
DdeI CTNAG 4 cut(s) 282, 449, 857, 1111
DpnI GATC 8 cut(s) 210, 303, 309, 423, 827, 971, 1052, 1562
DpnII GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
Ecl136II GAGCTC 1 cut(s) 854
Eco130I CCWWGG 6 cut(s) 79, 739, 1183, 1224, 1356, 1398
Eco147I AGGCCT 1 cut(s) 862
Eco24I GRGCYC 1 cut(s) 856
Eco31I GGTCTC 1 cut(s) 71
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 2 cut(s) 597, 1360
Eco53kI GAGCTC 1 cut(s) 854
Eco57I CTGAAG 2 cut(s) 468, 668
EcoICRI GAGCTC 1 cut(s) 854
EcoRII CCWGG 1 cut(s) 187
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 6 cut(s) 79, 739, 1183, 1224, 1356, 1398
EcoT38I GRGCYC 1 cut(s) 856
ErhI CCWWGG 6 cut(s) 79, 739, 1183, 1224, 1356, 1398
FaeI CATG 8 cut(s) 31, 90, 612, 826, 1061, 1381, 1402, 1604
FalI AAGNNNNNCTT 2 cut(s) 942, 974
FaqI GGGAC 3 cut(s) 469, 936, 1598
FatI CATG 8 cut(s) 27, 86, 608, 822, 1057, 1377, 1398, 1600
FauI CCCGC 2 cut(s) 138, 1768
FauNDI CATATG 2 cut(s) 556, 877
FblI GTMKAC 2 cut(s) 643, 1680
Fnu4HI GCNGC 4 cut(s) 236, 815, 1142, 1199
FokI GGATG 6 cut(s) 32, 651, 1263, 1472, 1583, 1649
FriOI GRGCYC 1 cut(s) 856
Fsp4HI GCNGC 4 cut(s) 236, 815, 1142, 1199
FspBI CTAG 5 cut(s) 264, 321, 459, 719, 1389
GlaI GCGC 2 cut(s) 1090, 1369
GluI GCNGC 4 cut(s) 236, 815, 1142, 1199
HaeIII GGCC 2 cut(s) 129, 862
HapII CCGG 2 cut(s) 1415, 1536
HgaI GACGC 1 cut(s) 1809
HhaI GCGC 2 cut(s) 1091, 1370
Hin1I GRCGYC 1 cut(s) 1801
Hin1II CATG 8 cut(s) 31, 90, 612, 826, 1061, 1381, 1402, 1604
Hin6I GCGC 2 cut(s) 1089, 1368
HinP1I GCGC 2 cut(s) 1089, 1368
HincII GTYRAC 2 cut(s) 499, 1063
HindII GTYRAC 2 cut(s) 499, 1063
HindIII AAGCTT 1 cut(s) 956
HinfI GANTC 6 cut(s) 107, 153, 289, 434, 774, 1263
HpaI GTTAAC 1 cut(s) 1063
HpaII CCGG 2 cut(s) 1415, 1536
HphI GGTGA 3 cut(s) 298, 399, 1235
Hpy166II GTNNAC 9 cut(s) 19, 57, 102, 407, 499, 644, 1063, 1465, 1681
Hpy188III TCNNGA 2 cut(s) 305, 1574
Hpy8I GTNNAC 9 cut(s) 19, 57, 102, 407, 499, 644, 1063, 1465, 1681
HpyAV CCTTC 6 cut(s) 338, 492, 548, 1277, 1698, 1712
HpyCH4IV ACGT 2 cut(s) 1065, 1731
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 213, 235, 1628
HpyF3I CTNAG 4 cut(s) 282, 449, 857, 1111
HpySE526I ACGT 2 cut(s) 1065, 1731
Hsp92I GRCGYC 1 cut(s) 1801
Hsp92II CATG 8 cut(s) 31, 90, 612, 826, 1061, 1381, 1402, 1604
HspAI GCGC 2 cut(s) 1089, 1368
KpnI GGTACC 2 cut(s) 1398, 1443
KspAI GTTAAC 1 cut(s) 1063
Kzo9I GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
LmnI GCTCC 3 cut(s) 83, 811, 1358
Lsp1109I GCAGC 3 cut(s) 222, 1128, 1185
LweI GCATC 4 cut(s) 978, 1605, 1609, 1671
MaeI CTAG 5 cut(s) 264, 321, 459, 719, 1389
MaeII ACGT 2 cut(s) 1065, 1731
MaeIII GTNAC 8 cut(s) 259, 383, 611, 724, 1253, 1482, 1519, 1815
MalI GATC 8 cut(s) 210, 303, 309, 423, 827, 971, 1052, 1562
MbiI CCGCTC 1 cut(s) 814
MboI GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
MfeI CAATTG 1 cut(s) 1644
MflI RGATCY 4 cut(s) 421, 969, 1050, 1560
MhlI GDGCHC 3 cut(s) 104, 856, 1355
MlyI GAGTC 2 cut(s) 147, 768
MmeI TCCRAC 5 cut(s) 751, 1028, 1060, 1437, 1695
MnlI CCTC 6 cut(s) 361, 623, 1007, 1296, 1600, 1752
MroXI GAANNNNTTC 2 cut(s) 958, 1178
MseI TTAA 5 cut(s) 75, 270, 339, 399, 1062
MslI CAYNNNNRTG 2 cut(s) 1251, 1275
MspA1I CMGCKG 1 cut(s) 1763
MspI CCGG 2 cut(s) 1415, 1536
MspR9I CCNGG 2 cut(s) 189, 1537
MunI CAATTG 1 cut(s) 1644
Mva1269I GAATGC 1 cut(s) 686
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 4 cut(s) 180, 213, 235, 1628
NciI CCSGG 1 cut(s) 1537
NcoI CCATGG 1 cut(s) 1398
NdeI CATATG 2 cut(s) 556, 877
NdeII GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
NlaIII CATG 8 cut(s) 31, 90, 612, 826, 1061, 1381, 1402, 1604
NlaIV GGNNCC 3 cut(s) 1052, 1396, 1441
NmuCI GTSAC 4 cut(s) 259, 383, 1253, 1519
NspI RCATGY 2 cut(s) 90, 612
NspV TTCGAA 1 cut(s) 567
OliI CACNNNNGTG 1 cut(s) 1251
PaeI GCATGC 1 cut(s) 90
PceI AGGCCT 1 cut(s) 862
PciI ACATGT 1 cut(s) 608
PctI GAATGC 1 cut(s) 686
PdmI GAANNNNTTC 2 cut(s) 958, 1178
PfeI GAWTC 4 cut(s) 107, 289, 434, 1263
PfoI TCCNGGA 1 cut(s) 187
PkrI GCNGC 4 cut(s) 237, 816, 1143, 1200
Ple19I CGATCG 1 cut(s) 211
PleI GAGTC 2 cut(s) 147, 768
PpsI GAGTC 2 cut(s) 147, 768
PscI ACATGT 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 856
Psp6I CCWGG 1 cut(s) 187
PspGI CCWGG 1 cut(s) 187
PspN4I GGNNCC 3 cut(s) 1052, 1396, 1441
PspPI GGNCC 3 cut(s) 128, 597, 1360
PsuI RGATCY 4 cut(s) 421, 969, 1050, 1560
PvuI CGATCG 1 cut(s) 211
RsaI GTAC 7 cut(s) 377, 523, 533, 706, 872, 1396, 1441
RsaNI GTAC 7 cut(s) 376, 522, 532, 705, 871, 1395, 1440
RseI CAYNNNNRTG 2 cut(s) 1251, 1275
SacI GAGCTC 1 cut(s) 856
SaqAI TTAA 5 cut(s) 75, 270, 339, 399, 1062
SatI GCNGC 4 cut(s) 236, 815, 1142, 1199
Sau3AI GATC 8 cut(s) 208, 301, 307, 421, 825, 969, 1050, 1560
Sau96I GGNCC 3 cut(s) 128, 597, 1360
SchI GAGTC 2 cut(s) 147, 768
ScrFI CCNGG 2 cut(s) 189, 1537
SduI GDGCHC 3 cut(s) 104, 856, 1355
SfaNI GCATC 4 cut(s) 978, 1605, 1609, 1671
SfcI CTRYAG 1 cut(s) 847
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 2 cut(s) 597, 1360
SmiMI CAYNNNNRTG 2 cut(s) 1251, 1275
SmlI CTYRAG 2 cut(s) 230, 1426
SmoI CTYRAG 2 cut(s) 230, 1426
SpeI ACTAGT 2 cut(s) 263, 718
SphI GCATGC 1 cut(s) 90
SseBI AGGCCT 1 cut(s) 862
SsiI CCGC 5 cut(s) 117, 131, 814, 892, 1761
SspMI CTAG 5 cut(s) 264, 321, 459, 719, 1389
SstI GAGCTC 1 cut(s) 856
StuI AGGCCT 1 cut(s) 862
StyD4I CCNGG 2 cut(s) 187, 1535
StyI CCWWGG 6 cut(s) 79, 739, 1183, 1224, 1356, 1398
TaiI ACGT 2 cut(s) 1068, 1734
TaqI TCGA 6 cut(s) 151, 211, 567, 828, 962, 1275
TaqII GACCGA 1 cut(s) 813
TatI WGTACW 1 cut(s) 531
TauI GCSGC 1 cut(s) 817
TfiI GAWTC 4 cut(s) 107, 289, 434, 1263
Tru1I TTAA 5 cut(s) 75, 270, 339, 399, 1062
Tru9I TTAA 5 cut(s) 75, 270, 339, 399, 1062
TscAI CASTG 6 cut(s) 119, 182, 386, 553, 948, 1673
TseFI GTSAC 4 cut(s) 259, 383, 1253, 1519
TseI GCWGC 3 cut(s) 235, 1141, 1198
Tsp45I GTSAC 4 cut(s) 259, 383, 1253, 1519
TspDTI ATGAA 8 cut(s) 34, 349, 735, 1044, 1134, 1141, 1259, 1709
TspGWI ACGGA 1 cut(s) 1263
TspRI CASTG 6 cut(s) 119, 182, 386, 553, 948, 1673
VneI GTGCAC 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 597, 1360
XapI RAATTY 5 cut(s) 7, 833, 905, 1121, 1147
XceI RCATGY 2 cut(s) 90, 612
XmiI GTMKAC 2 cut(s) 643, 1680
XmnI GAANNNNTTC 2 cut(s) 958, 1178
XspI CTAG 5 cut(s) 264, 321, 459, 719, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.