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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 44 |
| AluBI | AGCT | 1 cut(s) | 28 |
| AluI | AGCT | 1 cut(s) | 28 |
| Alw21I | GWGCWC | 1 cut(s) | 81 |
| AsuHPI | GGTGA | 2 cut(s) | 59, 86 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| Bbv12I | GWGCWC | 1 cut(s) | 81 |
| BmiI | GGNNCC | 1 cut(s) | 6 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 44 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 44 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BsiHKAI | GWGCWC | 1 cut(s) | 81 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 44 |
| Bsp1286I | GDGCHC | 1 cut(s) | 81 |
| BspLI | GGNNCC | 1 cut(s) | 6 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| CviJI | RGCY | 1 cut(s) | 28 |
| CviKI_1 | RGCY | 1 cut(s) | 28 |
| DraI | TTTAAA | 1 cut(s) | 66 |
| HphI | GGTGA | 2 cut(s) | 59, 86 |
| Hpy188III | TCNNGA | 1 cut(s) | 38 |
| LmnI | GCTCC | 1 cut(s) | 33 |
| LpnPI | CCDG | 2 cut(s) | 38, 51 |
| MhlI | GDGCHC | 1 cut(s) | 81 |
| MseI | TTAA | 1 cut(s) | 65 |
| MspJI | CNNR | 8 cut(s) | 20, 31, 37, 45, 50, 51, 58, 62 |
| NlaIV | GGNNCC | 1 cut(s) | 6 |
| PspN4I | GGNNCC | 1 cut(s) | 6 |
| SaqAI | TTAA | 1 cut(s) | 65 |
| SduI | GDGCHC | 1 cut(s) | 81 |
| SetI | ASST | 3 cut(s) | 10, 30, 72 |
| SgeI | CNNG | 3 cut(s) | 37, 45, 50 |
| Tru1I | TTAA | 1 cut(s) | 65 |
| Tru9I | TTAA | 1 cut(s) | 65 |