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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 116 |
| AcoI | YGGCCR | 1 cut(s) | 136 |
| AfaI | GTAC | 2 cut(s) | 53, 157 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 55, 271 |
| AgsI | TTSAA | 1 cut(s) | 19 |
| AjiI | CACGTC | 1 cut(s) | 206 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 72, 104 |
| AluBI | AGCT | 1 cut(s) | 353 |
| AluI | AGCT | 1 cut(s) | 353 |
| AoxI | GGCC | 1 cut(s) | 136 |
| ApeKI | GCWGC | 2 cut(s) | 311, 353 |
| AspLEI | GCGC | 2 cut(s) | 116, 118 |
| BaeGI | GKGCMC | 1 cut(s) | 266 |
| BalI | TGGCCA | 1 cut(s) | 138 |
| BbvI | GCAGC | 2 cut(s) | 298, 340 |
| BciVI | GTATCC | 1 cut(s) | 74 |
| BfmI | CTRYAG | 1 cut(s) | 255 |
| BfuI | GTATCC | 1 cut(s) | 74 |
| BisI | GCNGC | 2 cut(s) | 312, 354 |
| BlsI | GCNGC | 2 cut(s) | 313, 355 |
| BmgBI | CACGTC | 1 cut(s) | 206 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 308, 340 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 55, 271 |
| Bse1I | ACTGG | 2 cut(s) | 38, 254 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 55, 271 |
| BseMII | CTCAG | 1 cut(s) | 306 |
| BseNI | ACTGG | 2 cut(s) | 38, 254 |
| BsePI | GCGCGC | 1 cut(s) | 114 |
| BseSI | GKGCMC | 1 cut(s) | 266 |
| BseXI | GCAGC | 2 cut(s) | 298, 340 |
| Bsh1236I | CGCG | 1 cut(s) | 116 |
| BshFI | GGCC | 1 cut(s) | 138 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 55, 271 |
| BsmI | GAATGC | 1 cut(s) | 50 |
| BsnI | GGCC | 1 cut(s) | 138 |
| Bsp1286I | GDGCHC | 1 cut(s) | 266 |
| BspANI | GGCC | 1 cut(s) | 138 |
| BspCNI | CTCAG | 1 cut(s) | 307 |
| BspFNI | CGCG | 1 cut(s) | 116 |
| BspMAI | CTGCAG | 1 cut(s) | 259 |
| BsrI | ACTGG | 2 cut(s) | 38, 254 |
| BssHII | GCGCGC | 1 cut(s) | 114 |
| Bst4CI | ACNGT | 2 cut(s) | 122, 185 |
| BstC8I | GCNNGC | 1 cut(s) | 116 |
| BstDEI | CTNAG | 1 cut(s) | 315 |
| BstFNI | CGCG | 1 cut(s) | 116 |
| BstHHI | GCGC | 2 cut(s) | 116, 118 |
| BstSFI | CTRYAG | 1 cut(s) | 255 |
| BstSLI | GKGCMC | 1 cut(s) | 266 |
| BstUI | CGCG | 1 cut(s) | 116 |
| BstV1I | GCAGC | 2 cut(s) | 298, 340 |
| BsuI | GTATCC | 1 cut(s) | 74 |
| BsuRI | GGCC | 1 cut(s) | 138 |
| BtrI | CACGTC | 1 cut(s) | 206 |
| BtsIMutI | CAGTG | 3 cut(s) | 31, 118, 247 |
| Cac8I | GCNNGC | 1 cut(s) | 116 |
| CfoI | GCGC | 2 cut(s) | 116, 118 |
| Csp6I | GTAC | 2 cut(s) | 52, 156 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 205, 240 |
| CviJI | RGCY | 5 cut(s) | 99, 138, 289, 335, 353 |
| CviKI_1 | RGCY | 5 cut(s) | 99, 138, 289, 335, 353 |
| CviQI | GTAC | 2 cut(s) | 52, 156 |
| DdeI | CTNAG | 1 cut(s) | 315 |
| EaeI | YGGCCR | 1 cut(s) | 136 |
| FaiI | YATR | 2 cut(s) | 102, 149 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 72, 104, 260, 292 |
| Fnu4HI | GCNGC | 2 cut(s) | 312, 354 |
| Fsp4HI | GCNGC | 2 cut(s) | 312, 354 |
| GlaI | GCGC | 2 cut(s) | 115, 117 |
| GluI | GCNGC | 2 cut(s) | 312, 354 |
| HaeIII | GGCC | 1 cut(s) | 138 |
| HhaI | GCGC | 2 cut(s) | 116, 118 |
| Hin6I | GCGC | 2 cut(s) | 114, 116 |
| HinP1I | GCGC | 2 cut(s) | 114, 116 |
| HinfI | GANTC | 1 cut(s) | 295 |
| Hpy166II | GTNNAC | 1 cut(s) | 158 |
| Hpy188I | TCNGA | 1 cut(s) | 145 |
| Hpy8I | GTNNAC | 1 cut(s) | 158 |
| HpyAV | CCTTC | 4 cut(s) | 13, 124, 194, 265 |
| HpyCH4III | ACNGT | 2 cut(s) | 122, 185 |
| HpyCH4IV | ACGT | 1 cut(s) | 205 |
| HpyCH4V | TGCA | 1 cut(s) | 257 |
| HpyF3I | CTNAG | 1 cut(s) | 315 |
| HpySE526I | ACGT | 1 cut(s) | 205 |
| HspAI | GCGC | 2 cut(s) | 114, 116 |
| LpnPI | CCDG | 2 cut(s) | 19, 235 |
| Lsp1109I | GCAGC | 2 cut(s) | 298, 340 |
| MaeII | ACGT | 1 cut(s) | 205 |
| MaeIII | GTNAC | 1 cut(s) | 82 |
| MhlI | GDGCHC | 1 cut(s) | 266 |
| MlsI | TGGCCA | 1 cut(s) | 138 |
| MluCI | AATT | 1 cut(s) | 166 |
| MluNI | TGGCCA | 1 cut(s) | 138 |
| MlyI | GAGTC | 1 cut(s) | 304 |
| MmeI | TCCRAC | 1 cut(s) | 282 |
| MnlI | CCTC | 2 cut(s) | 186, 229 |
| Mox20I | TGGCCA | 1 cut(s) | 138 |
| MscI | TGGCCA | 1 cut(s) | 138 |
| MslI | CAYNNNNRTG | 1 cut(s) | 123 |
| Msp20I | TGGCCA | 1 cut(s) | 138 |
| Mva1269I | GAATGC | 1 cut(s) | 50 |
| MvnI | CGCG | 1 cut(s) | 116 |
| PauI | GCGCGC | 1 cut(s) | 114 |
| PctI | GAATGC | 1 cut(s) | 50 |
| PkrI | GCNGC | 2 cut(s) | 313, 355 |
| PleI | GAGTC | 1 cut(s) | 303 |
| PpsI | GAGTC | 1 cut(s) | 303 |
| PstI | CTGCAG | 1 cut(s) | 259 |
| PteI | GCGCGC | 1 cut(s) | 114 |
| RsaI | GTAC | 2 cut(s) | 53, 157 |
| RsaNI | GTAC | 2 cut(s) | 52, 156 |
| RseI | CAYNNNNRTG | 1 cut(s) | 123 |
| SatI | GCNGC | 2 cut(s) | 312, 354 |
| SchI | GAGTC | 1 cut(s) | 304 |
| SduI | GDGCHC | 1 cut(s) | 266 |
| SetI | ASST | 4 cut(s) | 197, 208, 222, 355 |
| SfcI | CTRYAG | 1 cut(s) | 255 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 123 |
| Sse9I | AATT | 1 cut(s) | 166 |
| TaaI | ACNGT | 2 cut(s) | 122, 185 |
| TaiI | ACGT | 1 cut(s) | 208 |
| TasI | AATT | 1 cut(s) | 166 |
| TscAI | CASTG | 3 cut(s) | 38, 125, 254 |
| TseI | GCWGC | 2 cut(s) | 311, 353 |
| TspDTI | ATGAA | 1 cut(s) | 57 |
| TspRI | CASTG | 3 cut(s) | 38, 125, 254 |