RLG00000020541

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000181
Physical Location & Seq
Forward (+)
66100378 .. 66104003
3626 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020541

Sequence Viewer

Length: 588 bp
ATGCGCGACGAGCATATAAAACAGCAGAGACCCAAATTTACACAACAATTGACGGCCATCTCTGCAATTCTGTTTCTCTTTCATCCAGACCCAGACGACGCCATGTCTCTTGTCGAATCAGAATTCTCCAAGGATCCGAACCCGAACCAGGTCCCGCCGGACGAGGACGACAAGATCCAGACTCATGGAAAAAACAAAGCTGCTGAAGACTCTCTGTCTCAACTTGAAGTAGAGGAGGAAGAGGAAGAGGAAGAGGAAGAGGAAGAGGAAGAAGAAGATGATGATGATGATGATGAAGAAGAAGATGAAGATGAAGAAGAAGCAGAAGCAGCAGAAGAAGAATTTGAGATCTCCTGGGAAACCTGCTTCATGGTTGGCCGAGATTGCGGATTGGTTTGCAAGCATGAAAATGCAAGCTTCACAATAAACCCCAGTTTCTCTGCGATTGGACCTCCAGGAGCTGAAGGCGTTGATCCAAAATGTCCCACAGTACTTGATGTCAATAATGAGGATGAGAGAACTGAGATCATGAGGTCCCCCGGCTGCTTTTCCATGATCACTTCTAGAGTTAACACTCCAAAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.11

Weight (kDa)

4.05

Isoelectric Point (pI)

68.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000181)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02310 FvH4_1g02340 FvH4_1g02340 FvH4_1g02340 FvH4_3g13110 FvH4_4g04530 FvH4_5g26960 FvH4_5g26990 FvH4_6g01091 FvH4_6g08071 FvH4_6g19630 FvH4_6g38111 FvH4_6g38112 FvH4_6g48801 FvH4_6g48810 FvH4_6g48861 FvH4_6g49020 FvH4_6g49040 FvH4_6g49040 FvH4_6g49060 FvH4_6g49070 FvH4_6g49070 FvH4_6g49870 FvH4_6g49880
malus_domestica MD05G1007300.v1.1 MD05G1007600.v1.1 MD08G1121900.v1.1 MD08G1122000.v1.1 MD08G1122200.v1.1 MD08G1122700.v1.1
prunus_persica Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G187100_v2.0.a1 Prupe.2G227700_v2.0.a1 Prupe.2G227800_v2.0.a1 Prupe.2G227900_v2.0.a1 Prupe.4G043000_v2.0.a1 Prupe.4G046400_v2.0.a1 Prupe.4G077400_v2.0.a1 Prupe.4G077500_v2.0.a1 Prupe.4G159200_v2.0.a1 Prupe.4G159300_v2.0.a1 Prupe.4G159500_v2.0.a1 Prupe.6G311400_v2.0.a1 Prupe.6G311400_v2.0.a1
pyrus_communis pycom05g00390 pycom07g27750 pycom08g10150 pycom08g10170
rosa_chinensis RchiOBHm_Chr1g0328161 RchiOBHm_Chr1g0372961 RchiOBHm_Chr2g0131001 RchiOBHm_Chr2g0151211 RchiOBHm_Chr2g0151231 RchiOBHm_Chr2g0151281 RchiOBHm_Chr2g0151321 RchiOBHm_Chr2g0170041 RchiOBHm_Chr3g0457761 RchiOBHm_Chr3g0457871 RchiOBHm_Chr5g0014841 RchiOBHm_Chr5g0021371 RchiOBHm_Chr5g0038581 RchiOBHm_Chr5g0038691 RchiOBHm_Chr5g0038761 RchiOBHm_Chr6g0246131 RchiOBHm_Chr6g0246181
rosa_laevigata RLG00000015270 RLG00000020541 RLG00000021919 RLG00000025188 RLG00000032146 RLG00000032147 RLG00000032607 RLG00000033860
rosa_multiflora Rmu_sc0001102.1_g000003 Rmu_sc0002298.1_g000007 Rmu_sc0002516.1_g000005 Rmu_sc0002516.1_g000013 Rmu_sc0003629.1_g000050 Rmu_sc0023055.1_g000001
rosa_roxburghii Rroxscaffold_1G00042380 Rroxscaffold_1G00042450 Rroxscaffold_1G00042460 Rroxscaffold_1G00042550 Rroxscaffold_1G00061700 Rroxscaffold_1G00061730 Rroxscaffold_1G00063310 Rroxscaffold_1G00065700 Rroxscaffold_2G00096530 Rroxscaffold_2G00096560 Rroxscaffold_2G00096590 Rroxscaffold_2G00096660 Rroxscaffold_2G00101110 Rroxscaffold_2G00113390 Rroxscaffold_5G00339030 Rroxscaffold_5G00351680 Rroxscaffold_6G00421510 Rroxscaffold_7G00168250 Rroxscaffold_7G00214760 Rroxscaffold_7G00214840
rosa_rugosa Rorug02G0294400 Rorug02G0545100 Rorug05G0008000 Rorug05G0066600 Rorug05G0173700 Rorug05G0173800 Rorug05G0290700 Rorug05G0290800 Rorug05G0290800 Rorug05G0519600 Rorug05G0519700
rosa_samantha Rh1CG087500 Rh1DG094400 Rh2AG570900 Rh2AG612800 Rh2BG354000 Rh2BG498100 Rh2BG498500 Rh2BG498600 Rh2BG628900 Rh2DG372100 Rh2DG509000 Rh2DG509300 Rh2DG509400 Rh2DG509600 Rh2DG640700 Rh3AG083900 Rh3BG086400 Rh3CG086700 Rh5AG102700 Rh5AG259700 Rh5AG261200 Rh5BG098700 Rh5BG098800 Rh5BG099500 Rh5BG113500 Rh5BG113900 Rh5BG155300 Rh5BG264600 Rh5CG111100 Rh5CG125200 Rh5CG169000 Rh5CG297600 Rh5DG098300 Rh5DG272900 Rh6AG033700 Rh6BG028900 Rh6CG029100 Rh6CG029200 Rh6DG028200 Rh6DG028300 Rh7CG429700
rosa_wichuraiana Rw0G009330 Rw0G012230 Rw0G018980 Rw1G008830 Rw2G035660 Rw2G039830 Rw2G040230 Rw2G040240 Rw5G013700 Rw5G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 371
AccII CGCG 1 cut(s) 6
AciI CCGC 2 cut(s) 155, 387
AclWI GGATC 4 cut(s) 128, 141, 169, 467
AcoI YGGCCR 2 cut(s) 54, 376
AcsI RAATTY 3 cut(s) 35, 122, 341
AcuI CTGAAG 2 cut(s) 225, 483
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 1 cut(s) 492
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 1 cut(s) 227
AhdI GACNNNNNGTC 2 cut(s) 103, 214
AjnI CCWGG 3 cut(s) 147, 353, 454
AluBI AGCT 3 cut(s) 200, 417, 461
AluI AGCT 3 cut(s) 200, 417, 461
Alw26I GTCTC 3 cut(s) 22, 111, 222
AlwI GGATC 4 cut(s) 128, 141, 169, 467
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 2 cut(s) 54, 376
ApeKI GCWGC 3 cut(s) 200, 329, 543
ApoI RAATTY 3 cut(s) 35, 122, 341
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 3 cut(s) 151, 449, 534
AsuC2I CCSGG 1 cut(s) 540
AvaII GGWCC 3 cut(s) 151, 449, 534
BamHI GGATCC 1 cut(s) 133
BbsI GAAGAC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 187, 341, 530
BccI CCATC 1 cut(s) 65
BceAI ACGGC 1 cut(s) 69
BciT130I CCWGG 3 cut(s) 149, 355, 456
BclI TGATCA 1 cut(s) 555
BcnI CCSGG 1 cut(s) 540
BcoDI GTCTC 3 cut(s) 22, 111, 222
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 348
BisI GCNGC 3 cut(s) 201, 330, 544
BlsI GCNGC 3 cut(s) 202, 331, 545
BmcAI AGTACT 1 cut(s) 492
Bme1390I CCNGG 4 cut(s) 149, 355, 456, 540
Bme18I GGWCC 3 cut(s) 151, 449, 534
BmeRI GACNNNNNGTC 2 cut(s) 103, 214
BmgT120I GGNCC 3 cut(s) 151, 449, 534
BmiI GGNNCC 3 cut(s) 135, 153, 536
BmrFI CCNGG 4 cut(s) 149, 355, 456, 540
BmrI ACTGGG 1 cut(s) 426
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 1 cut(s) 213
BpmI CTGGAG 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 540
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 22
BsaJI CCNNGG 3 cut(s) 129, 354, 538
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse1I ACTGG 1 cut(s) 432
BseBI CCWGG 3 cut(s) 149, 355, 456
BseDI CCNNGG 3 cut(s) 129, 354, 538
BseGI GGATG 2 cut(s) 82, 517
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 513
BseNI ACTGG 1 cut(s) 432
BseRI GAGGAG 1 cut(s) 248
BseXI GCAGC 3 cut(s) 187, 341, 530
Bsh1236I CGCG 1 cut(s) 6
BshFI GGCC 2 cut(s) 56, 378
BsiSI CCGG 2 cut(s) 158, 540
BslFI GGGAC 3 cut(s) 137, 468, 520
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 3 cut(s) 22, 111, 222
BsmFI GGGAC 3 cut(s) 137, 468, 520
BsnI GGCC 2 cut(s) 56, 378
Bso31I GGTCTC 1 cut(s) 22
Bsp143I GATC 6 cut(s) 133, 174, 348, 472, 525, 555
BspACI CCGC 2 cut(s) 155, 387
BspANI GGCC 2 cut(s) 56, 378
BspCNI CTCAG 1 cut(s) 514
BspFNI CGCG 1 cut(s) 6
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 3 cut(s) 135, 153, 536
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 4 cut(s) 128, 141, 169, 467
BspTNI GGTCTC 1 cut(s) 22
BsrI ACTGG 1 cut(s) 432
BssECI CCNNGG 3 cut(s) 129, 354, 538
BssMI GATC 6 cut(s) 133, 174, 348, 472, 525, 555
BssNI GRCGYC 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 129
Bst2UI CCWGG 3 cut(s) 149, 355, 456
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 5 cut(s) 234, 240, 246, 252, 258
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 401, 415
BstDEI CTNAG 1 cut(s) 522
BstF5I GGATG 2 cut(s) 82, 517
BstFNI CGCG 1 cut(s) 6
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 6 cut(s) 136, 177, 351, 475, 528, 558
BstMAI GTCTC 3 cut(s) 22, 111, 222
BstMBI GATC 6 cut(s) 133, 174, 348, 472, 525, 555
BstMWI GCNNNNNNNGC 4 cut(s) 10, 62, 329, 384
BstNI CCWGG 3 cut(s) 149, 355, 456
BstSCI CCNGG 4 cut(s) 147, 353, 454, 538
BstSFI CTRYAG 1 cut(s) 584
BstUI CGCG 1 cut(s) 6
BstV1I GCAGC 3 cut(s) 187, 341, 530
BstV2I GAAGAC 1 cut(s) 213
BstX2I RGATCY 3 cut(s) 133, 174, 348
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 3 cut(s) 133, 174, 348
BsuRI GGCC 2 cut(s) 56, 378
BtsCI GGATG 2 cut(s) 82, 517
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 401, 415
CaiI CAGNNNCTG 1 cut(s) 461
CciI TCATGA 1 cut(s) 528
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 3 cut(s) 151, 449, 534
CseI GACGC 1 cut(s) 107
CsiI ACCWGGT 1 cut(s) 147
Csp6I GTAC 1 cut(s) 491
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 6 cut(s) 103, 185, 370, 404, 529, 553
CviJI RGCY 6 cut(s) 56, 200, 378, 417, 461, 543
CviKI_1 RGCY 6 cut(s) 56, 200, 378, 417, 461, 543
CviQI GTAC 1 cut(s) 491
DdeI CTNAG 1 cut(s) 522
DpnI GATC 6 cut(s) 135, 176, 350, 474, 527, 557
DpnII GATC 6 cut(s) 133, 174, 348, 472, 525, 555
DriI GACNNNNNGTC 2 cut(s) 103, 214
EaeI YGGCCR 2 cut(s) 54, 376
Eam1104I CTCTTC 5 cut(s) 234, 240, 246, 252, 258
Eam1105I GACNNNNNGTC 2 cut(s) 103, 214
EarI CTCTTC 5 cut(s) 234, 240, 246, 252, 258
Eco130I CCWWGG 1 cut(s) 129
Eco31I GGTCTC 1 cut(s) 22
Eco47I GGWCC 3 cut(s) 151, 449, 534
Eco57I CTGAAG 2 cut(s) 225, 483
EcoO109I RGGNCCY 2 cut(s) 151, 534
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 3 cut(s) 147, 353, 454
EcoT14I CCWWGG 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 129
FaeI CATG 6 cut(s) 106, 188, 373, 407, 532, 556
FaiI YATR 9 cut(s) 15, 17, 104, 186, 371, 405, 530, 554, 586
FaqI GGGAC 3 cut(s) 137, 468, 520
FatI CATG 6 cut(s) 102, 184, 369, 403, 528, 552
FauI CCCGC 1 cut(s) 162
FbaI TGATCA 1 cut(s) 555
Fnu4HI GCNGC 3 cut(s) 201, 330, 544
FokI GGATG 2 cut(s) 69, 524
Fsp4HI GCNGC 3 cut(s) 201, 330, 544
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 201, 330, 544
GsuI CTGGAG 1 cut(s) 438
HaeIII GGCC 2 cut(s) 56, 378
HapII CCGG 2 cut(s) 158, 540
HgaI GACGC 1 cut(s) 107
HhaI GCGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 6 cut(s) 106, 188, 373, 407, 532, 556
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 3 cut(s) 116, 181, 209
HpaI GTTAAC 1 cut(s) 571
HpaII CCGG 2 cut(s) 158, 540
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 2 cut(s) 121, 138
Hpy188III TCNNGA 4 cut(s) 86, 178, 529, 564
Hpy8I GTNNAC 1 cut(s) 571
Hpy99I CGWCG 2 cut(s) 11, 101
HpyAV CCTTC 1 cut(s) 458
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4V TGCA 3 cut(s) 65, 399, 413
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 62, 329, 384
HpyF3I CTNAG 1 cut(s) 522
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 6 cut(s) 106, 188, 373, 407, 532, 556
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 555
KspAI GTTAAC 1 cut(s) 571
Kzo9I GATC 6 cut(s) 133, 174, 348, 472, 525, 555
LmnI GCTCC 1 cut(s) 458
Lsp1109I GCAGC 3 cut(s) 187, 341, 530
MabI ACCWGGT 1 cut(s) 147
MaeI CTAG 1 cut(s) 564
MalI GATC 6 cut(s) 135, 176, 350, 474, 527, 557
MboI GATC 6 cut(s) 133, 174, 348, 472, 525, 555
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 3 cut(s) 133, 174, 348
MluCI AATT 5 cut(s) 35, 47, 66, 122, 341
MlyI GAGTC 2 cut(s) 175, 203
MseI TTAA 1 cut(s) 570
MslI CAYNNNNRTG 1 cut(s) 408
MspI CCGG 2 cut(s) 158, 540
MspR9I CCNGG 4 cut(s) 149, 355, 456, 540
MunI CAATTG 1 cut(s) 47
MvaI CCWGG 3 cut(s) 149, 355, 456
MvnI CGCG 1 cut(s) 6
MwoI GCNNNNNNNGC 4 cut(s) 10, 62, 329, 384
NciI CCSGG 1 cut(s) 540
NdeII GATC 6 cut(s) 133, 174, 348, 472, 525, 555
NlaIII CATG 6 cut(s) 106, 188, 373, 407, 532, 556
NlaIV GGNNCC 3 cut(s) 135, 153, 536
NmeAIII GCCGAG 1 cut(s) 404
PagI TCATGA 1 cut(s) 528
PfeI GAWTC 1 cut(s) 116
PfoI TCCNGGA 1 cut(s) 454
PkrI GCNGC 3 cut(s) 202, 331, 545
PleI GAGTC 2 cut(s) 175, 203
PpsI GAGTC 2 cut(s) 175, 203
PpuMI RGGWCCY 2 cut(s) 151, 534
Psp5II RGGWCCY 2 cut(s) 151, 534
Psp6I CCWGG 3 cut(s) 147, 353, 454
PspGI CCWGG 3 cut(s) 147, 353, 454
PspN4I GGNNCC 3 cut(s) 135, 153, 536
PspPI GGNCC 3 cut(s) 151, 449, 534
PspPPI RGGWCCY 2 cut(s) 151, 534
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 3 cut(s) 133, 174, 348
RsaI GTAC 1 cut(s) 492
RsaNI GTAC 1 cut(s) 491
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 1 cut(s) 570
SatI GCNGC 3 cut(s) 201, 330, 544
Sau3AI GATC 6 cut(s) 133, 174, 348, 472, 525, 555
Sau96I GGNCC 3 cut(s) 151, 449, 534
ScaI AGTACT 1 cut(s) 492
SchI GAGTC 2 cut(s) 175, 203
ScrFI CCNGG 4 cut(s) 149, 355, 456, 540
SetI ASST 7 cut(s) 153, 202, 365, 419, 454, 463, 536
SexAI ACCWGGT 1 cut(s) 147
SfcI CTRYAG 1 cut(s) 584
SinI GGWCC 3 cut(s) 151, 449, 534
SmiMI CAYNNNNRTG 1 cut(s) 408
Sse9I AATT 5 cut(s) 35, 47, 66, 122, 341
SsiI CCGC 2 cut(s) 155, 387
SspMI CTAG 1 cut(s) 564
StyD4I CCNGG 4 cut(s) 147, 353, 454, 538
StyI CCWWGG 1 cut(s) 129
TaaI ACNGT 1 cut(s) 490
TaqI TCGA 1 cut(s) 114
TasI AATT 5 cut(s) 35, 47, 66, 122, 341
TatI WGTACW 1 cut(s) 490
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 570
Tru9I TTAA 1 cut(s) 570
TseI GCWGC 3 cut(s) 200, 329, 543
TspDTI ATGAA 6 cut(s) 71, 309, 321, 327, 358, 420
VpaK11BI GGWCC 3 cut(s) 151, 449, 534
XapI RAATTY 3 cut(s) 35, 122, 341
XbaI TCTAGA 1 cut(s) 563
XspI CTAG 1 cut(s) 564
ZrmI AGTACT 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.