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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 198 |
| AcoI | YGGCCR | 2 cut(s) | 144, 169 |
| AgsI | TTSAA | 1 cut(s) | 123 |
| AluBI | AGCT | 1 cut(s) | 98 |
| AluI | AGCT | 1 cut(s) | 98 |
| AoxI | GGCC | 2 cut(s) | 144, 169 |
| BalI | TGGCCA | 2 cut(s) | 146, 171 |
| BsaJI | CCNNGG | 1 cut(s) | 60 |
| BseDI | CCNNGG | 1 cut(s) | 60 |
| BshFI | GGCC | 2 cut(s) | 146, 171 |
| BsmI | GAATGC | 1 cut(s) | 24 |
| BsnI | GGCC | 2 cut(s) | 146, 171 |
| Bsp19I | CCATGG | 1 cut(s) | 60 |
| BspANI | GGCC | 2 cut(s) | 146, 171 |
| BssECI | CCNNGG | 1 cut(s) | 60 |
| BssT1I | CCWWGG | 1 cut(s) | 60 |
| Bst4CI | ACNGT | 1 cut(s) | 196 |
| BstDSI | CCRYGG | 1 cut(s) | 60 |
| BsuRI | GGCC | 2 cut(s) | 146, 171 |
| BtgI | CCRYGG | 1 cut(s) | 60 |
| CviAII | CATG | 2 cut(s) | 43, 61 |
| CviJI | RGCY | 4 cut(s) | 41, 98, 146, 171 |
| CviKI_1 | RGCY | 4 cut(s) | 41, 98, 146, 171 |
| EaeI | YGGCCR | 2 cut(s) | 144, 169 |
| Eco130I | CCWWGG | 1 cut(s) | 60 |
| EcoT14I | CCWWGG | 1 cut(s) | 60 |
| ErhI | CCWWGG | 1 cut(s) | 60 |
| FaeI | CATG | 2 cut(s) | 46, 64 |
| FaiI | YATR | 2 cut(s) | 44, 62 |
| FatI | CATG | 2 cut(s) | 42, 60 |
| FblI | GTMKAC | 1 cut(s) | 198 |
| HaeIII | GGCC | 2 cut(s) | 146, 171 |
| Hin1II | CATG | 2 cut(s) | 46, 64 |
| HinfI | GANTC | 1 cut(s) | 175 |
| Hpy166II | GTNNAC | 1 cut(s) | 199 |
| Hpy188III | TCNNGA | 1 cut(s) | 12 |
| Hpy8I | GTNNAC | 1 cut(s) | 199 |
| HpyCH4III | ACNGT | 1 cut(s) | 196 |
| HpyCH4IV | ACGT | 1 cut(s) | 8 |
| HpyCH4V | TGCA | 2 cut(s) | 140, 165 |
| HpySE526I | ACGT | 1 cut(s) | 8 |
| Hsp92II | CATG | 2 cut(s) | 46, 64 |
| LpnPI | CCDG | 2 cut(s) | 25, 185 |
| MaeII | ACGT | 1 cut(s) | 8 |
| MlsI | TGGCCA | 2 cut(s) | 146, 171 |
| MluNI | TGGCCA | 2 cut(s) | 146, 171 |
| MmeI | TCCRAC | 1 cut(s) | 27 |
| MnlI | CCTC | 1 cut(s) | 113 |
| Mox20I | TGGCCA | 2 cut(s) | 146, 171 |
| MscI | TGGCCA | 2 cut(s) | 146, 171 |
| Msp20I | TGGCCA | 2 cut(s) | 146, 171 |
| Mva1269I | GAATGC | 1 cut(s) | 24 |
| NcoI | CCATGG | 1 cut(s) | 60 |
| NlaIII | CATG | 2 cut(s) | 46, 64 |
| PctI | GAATGC | 1 cut(s) | 24 |
| PfeI | GAWTC | 1 cut(s) | 175 |
| SetI | ASST | 2 cut(s) | 11, 100 |
| SgeI | CNNG | 5 cut(s) | 24, 55, 73, 125, 184 |
| StyI | CCWWGG | 1 cut(s) | 60 |
| TaaI | ACNGT | 1 cut(s) | 196 |
| TaiI | ACGT | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 66 |
| TfiI | GAWTC | 1 cut(s) | 175 |
| TspDTI | ATGAA | 1 cut(s) | 90 |
| TspGWI | ACGGA | 1 cut(s) | 194 |
| XmiI | GTMKAC | 1 cut(s) | 198 |