RLG00000032607

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000181
Physical Location & Seq
Forward (+)
16938737 .. 16944165
5429 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032607

Sequence Viewer

Length: 828 bp
ATGCTTTGGGTCGTTGGAGGACGACCGCTTGAGTCACAGCTGCACGGTGGAGGAGAAGTAGTAGTTGGCGAAGGGCGACGACCCGACCCGTTTGAATCATTGGGATCCCGTTCTCCACCAGCTGCCGTTCTCCGCCGCCTCGCCCGGGATTATCCTGATACCGTGAAGATTCCAGATCCATTGGCGGACACTACTACTTCTACATCTGCCTCTCCCTACCCTGAAGCACCCCTGGCTGCTCCCCTGACTCCTCGCCCTTTAAGTTTTTTTGACGATGCATATACCATACAGAAGCTCCAAACAGAAAAATGTATGTATTGTACAAAGGTACACGGTATTTCATCTTGCTCCTACACGAGACGTGTCCCAAAGAATGCACCTGTTGGCCCTGGCTGTGAACTAGTTTGTAGGATCTGTGAAAGTCCGTTTTCAGGCACCTGTTGTGGTCTAGATGAAGGCCGTGCTATTCTCAAAGAGTGCATAATTTGTGGAGCGTACGGTGACCACTGGCCTAGATGGTGCCCACGCAACAATCGAGTCGACAAGAAACTTTTAGACCTTCCTCGTGGTGTCTTTTACGTGGATTTCTCACGCGGTGCTAGTGCTCCTGAAAAAGTTGAACAGAAGGAGCCTGCAAAGCCAGCTTCAGTTGAGAGCAATGCTGTCAAAGATGATGACCCAGAGGATGATCATGACAGTGACGATGAAGATGATGAAACCCCTACTCCTAAGAAGCCTGGATCTAAGAGAAGAGCCCAAGCCTCTGCATCAGCTAAAACTCCAGTTTCTGCTCAGAAGGCAAATATAGATACTGGGTGCCCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

276

Amino Acids

29.84

Weight (kDa)

5.56

Isoelectric Point (pI)

54.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000181)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02310 FvH4_1g02340 FvH4_1g02340 FvH4_1g02340 FvH4_3g13110 FvH4_4g04530 FvH4_5g26960 FvH4_5g26990 FvH4_6g01091 FvH4_6g08071 FvH4_6g19630 FvH4_6g38111 FvH4_6g38112 FvH4_6g48801 FvH4_6g48810 FvH4_6g48861 FvH4_6g49020 FvH4_6g49040 FvH4_6g49040 FvH4_6g49060 FvH4_6g49070 FvH4_6g49070 FvH4_6g49870 FvH4_6g49880
malus_domestica MD05G1007300.v1.1 MD05G1007600.v1.1 MD08G1121900.v1.1 MD08G1122000.v1.1 MD08G1122200.v1.1 MD08G1122700.v1.1
prunus_persica Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G187100_v2.0.a1 Prupe.2G227700_v2.0.a1 Prupe.2G227800_v2.0.a1 Prupe.2G227900_v2.0.a1 Prupe.4G043000_v2.0.a1 Prupe.4G046400_v2.0.a1 Prupe.4G077400_v2.0.a1 Prupe.4G077500_v2.0.a1 Prupe.4G159200_v2.0.a1 Prupe.4G159300_v2.0.a1 Prupe.4G159500_v2.0.a1 Prupe.6G311400_v2.0.a1 Prupe.6G311400_v2.0.a1
pyrus_communis pycom05g00390 pycom07g27750 pycom08g10150 pycom08g10170
rosa_chinensis RchiOBHm_Chr1g0328161 RchiOBHm_Chr1g0372961 RchiOBHm_Chr2g0131001 RchiOBHm_Chr2g0151211 RchiOBHm_Chr2g0151231 RchiOBHm_Chr2g0151281 RchiOBHm_Chr2g0151321 RchiOBHm_Chr2g0170041 RchiOBHm_Chr3g0457761 RchiOBHm_Chr3g0457871 RchiOBHm_Chr5g0014841 RchiOBHm_Chr5g0021371 RchiOBHm_Chr5g0038581 RchiOBHm_Chr5g0038691 RchiOBHm_Chr5g0038761 RchiOBHm_Chr6g0246131 RchiOBHm_Chr6g0246181
rosa_laevigata RLG00000015270 RLG00000020541 RLG00000021919 RLG00000025188 RLG00000032146 RLG00000032147 RLG00000032607 RLG00000033860
rosa_multiflora Rmu_sc0001102.1_g000003 Rmu_sc0002298.1_g000007 Rmu_sc0002516.1_g000005 Rmu_sc0002516.1_g000013 Rmu_sc0003629.1_g000050 Rmu_sc0023055.1_g000001
rosa_roxburghii Rroxscaffold_1G00042380 Rroxscaffold_1G00042450 Rroxscaffold_1G00042460 Rroxscaffold_1G00042550 Rroxscaffold_1G00061700 Rroxscaffold_1G00061730 Rroxscaffold_1G00063310 Rroxscaffold_1G00065700 Rroxscaffold_2G00096530 Rroxscaffold_2G00096560 Rroxscaffold_2G00096590 Rroxscaffold_2G00096660 Rroxscaffold_2G00101110 Rroxscaffold_2G00113390 Rroxscaffold_5G00339030 Rroxscaffold_5G00351680 Rroxscaffold_6G00421510 Rroxscaffold_7G00168250 Rroxscaffold_7G00214760 Rroxscaffold_7G00214840
rosa_rugosa Rorug02G0294400 Rorug02G0545100 Rorug05G0008000 Rorug05G0066600 Rorug05G0173700 Rorug05G0173800 Rorug05G0290700 Rorug05G0290800 Rorug05G0290800 Rorug05G0519600 Rorug05G0519700
rosa_samantha Rh1CG087500 Rh1DG094400 Rh2AG570900 Rh2AG612800 Rh2BG354000 Rh2BG498100 Rh2BG498500 Rh2BG498600 Rh2BG628900 Rh2DG372100 Rh2DG509000 Rh2DG509300 Rh2DG509400 Rh2DG509600 Rh2DG640700 Rh3AG083900 Rh3BG086400 Rh3CG086700 Rh5AG102700 Rh5AG259700 Rh5AG261200 Rh5BG098700 Rh5BG098800 Rh5BG099500 Rh5BG113500 Rh5BG113900 Rh5BG155300 Rh5BG264600 Rh5CG111100 Rh5CG125200 Rh5CG169000 Rh5CG297600 Rh5DG098300 Rh5DG272900 Rh6AG033700 Rh6BG028900 Rh6CG029100 Rh6CG029200 Rh6DG028200 Rh6DG028300 Rh7CG429700
rosa_wichuraiana Rw0G009330 Rw0G012230 Rw0G018980 Rw1G008830 Rw2G035660 Rw2G039830 Rw2G040230 Rw2G040240 Rw5G013700 Rw5G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 434, 519, 816
AccI GTMKAC 1 cut(s) 540
AccII CGCG 1 cut(s) 594
AciI CCGC 5 cut(s) 26, 133, 136, 185, 594
AclWI GGATC 5 cut(s) 99, 112, 170, 419, 748
AcuI CTGAAG 2 cut(s) 243, 630
AdeI CACNNNGTG 1 cut(s) 596
AfaI GTAC 3 cut(s) 322, 330, 497
AfiI CCNNNNNNNGG 2 cut(s) 145, 431
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 95, 620
AhlI ACTAGT 1 cut(s) 400
AjiI CACGTC 1 cut(s) 362
AjnI CCWGG 3 cut(s) 231, 388, 736
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
AluBI AGCT 5 cut(s) 40, 122, 295, 644, 773
AluI AGCT 5 cut(s) 40, 122, 295, 644, 773
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 1 cut(s) 352
AlwI GGATC 5 cut(s) 99, 112, 170, 419, 748
AlwNI CAGNNNCTG 1 cut(s) 788
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 3 cut(s) 385, 457, 509
ApeKI GCWGC 3 cut(s) 40, 122, 236
AspS9I GGNCC 1 cut(s) 386
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 144
BaeGI GKGCMC 2 cut(s) 524, 821
BamHI GGATCC 1 cut(s) 104
BanI GGYRCC 3 cut(s) 434, 519, 816
BanII GRGCYC 1 cut(s) 757
BauI CACGAG 2 cut(s) 355, 564
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 3 cut(s) 27, 109, 223
BccI CCATC 1 cut(s) 510
BceAI ACGGC 2 cut(s) 110, 444
BciT130I CCWGG 3 cut(s) 233, 390, 738
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 1 cut(s) 352
BcuI ACTAGT 1 cut(s) 400
BfaI CTAG 4 cut(s) 401, 449, 513, 600
BisI GCNGC 4 cut(s) 41, 123, 136, 237
BlsI GCNGC 4 cut(s) 42, 124, 137, 238
Bme1390I CCNGG 5 cut(s) 145, 146, 233, 390, 738
BmeT110I CYCGRG 1 cut(s) 144
BmgBI CACGTC 1 cut(s) 362
BmgT120I GGNCC 1 cut(s) 386
BmiI GGNNCC 5 cut(s) 106, 436, 521, 630, 818
BmrFI CCNGG 5 cut(s) 145, 146, 233, 390, 738
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 2 cut(s) 265, 776
BmuI ACTGGG 1 cut(s) 822
BpmI CTGGAG 1 cut(s) 765
BpuEI CTTGAG 1 cut(s) 50
BpuMI CCSGG 2 cut(s) 145, 146
BsaAI YACGTR 1 cut(s) 580
BsaJI CCNNGG 3 cut(s) 144, 231, 388
BsaXI ACNNNNNCTCC 8 cut(s) 42, 45, 72, 75, 279, 309, 709, 739
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 431
Bse1I ACTGG 3 cut(s) 512, 782, 817
Bse3DI GCAATG 1 cut(s) 664
BseBI CCWGG 3 cut(s) 233, 390, 738
BseDI CCNNGG 3 cut(s) 144, 231, 388
BseGI GGATG 1 cut(s) 691
BseLI CCNNNNNNNGG 2 cut(s) 145, 431
BseMI GCAATG 1 cut(s) 664
BseMII CTCAG 1 cut(s) 806
BseNI ACTGG 3 cut(s) 512, 782, 817
BseRI GAGGAG 2 cut(s) 66, 240
BseSI GKGCMC 2 cut(s) 524, 821
BseXI GCAGC 3 cut(s) 27, 109, 223
BsgI GTGCAG 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 594
Bsh1285I CGRYCG 1 cut(s) 26
BshFI GGCC 3 cut(s) 387, 459, 511
BshNI GGYRCC 3 cut(s) 434, 519, 816
BsiEI CGRYCG 1 cut(s) 26
BsiHKAI GWGCWC 1 cut(s) 607
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 1 cut(s) 145
BsiWI CGTACG 1 cut(s) 495
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 2 cut(s) 145, 431
BsmAI GTCTC 1 cut(s) 352
BsmBI CGTCTC 1 cut(s) 352
BsmFI GGGAC 1 cut(s) 350
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 3 cut(s) 387, 459, 511
BsoBI CYCGRG 1 cut(s) 144
Bsp1286I GDGCHC 4 cut(s) 524, 607, 757, 821
Bsp1407I TGTACA 1 cut(s) 320
Bsp143I GATC 5 cut(s) 104, 175, 411, 688, 740
BspACI CCGC 5 cut(s) 26, 133, 136, 185, 594
BspANI GGCC 3 cut(s) 387, 459, 511
BspCNI CTCAG 1 cut(s) 805
BspFNI CGCG 1 cut(s) 594
BspHI TCATGA 1 cut(s) 691
BspLI GGNNCC 5 cut(s) 106, 436, 521, 630, 818
BspPI GGATC 5 cut(s) 99, 112, 170, 419, 748
BspQI GCTCTTC 1 cut(s) 745
BspT107I GGYRCC 3 cut(s) 434, 519, 816
BsrDI GCAATG 1 cut(s) 664
BsrGI TGTACA 1 cut(s) 320
BsrI ACTGG 3 cut(s) 512, 782, 817
BssECI CCNNGG 3 cut(s) 144, 231, 388
BssMI GATC 5 cut(s) 104, 175, 411, 688, 740
BssSI CACGAG 2 cut(s) 355, 564
Bst2BI CACGAG 2 cut(s) 355, 564
Bst2UI CCWGG 3 cut(s) 233, 390, 738
Bst4CI ACNGT 5 cut(s) 47, 163, 335, 500, 698
Bst6I CTCTTC 1 cut(s) 745
BstAUI TGTACA 1 cut(s) 320
BstBAI YACGTR 1 cut(s) 580
BstC8I GCNNGC 2 cut(s) 633, 642
BstDEI CTNAG 3 cut(s) 729, 744, 792
BstEII GGTNACC 1 cut(s) 500
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 1 cut(s) 594
BstKTI GATC 5 cut(s) 107, 178, 414, 691, 743
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 5 cut(s) 104, 175, 411, 688, 740
BstMCI CGRYCG 1 cut(s) 26
BstMWI GCNNNNNNNGC 4 cut(s) 233, 637, 641, 797
BstNI CCWGG 3 cut(s) 233, 390, 738
BstPI GGTNACC 1 cut(s) 500
BstSCI CCNGG 5 cut(s) 143, 144, 231, 388, 736
BstSLI GKGCMC 2 cut(s) 524, 821
BstUI CGCG 1 cut(s) 594
BstV1I GCAGC 3 cut(s) 27, 109, 223
BstX2I RGATCY 4 cut(s) 104, 175, 411, 740
BstYI RGATCY 4 cut(s) 104, 175, 411, 740
BsuRI GGCC 3 cut(s) 387, 459, 511
BtrI CACGTC 1 cut(s) 362
BtsCI GGATG 1 cut(s) 691
BtsIMutI CAGTG 2 cut(s) 505, 703
Cac8I GCNNGC 2 cut(s) 633, 642
CaiI CAGNNNCTG 1 cut(s) 788
CciI TCATGA 1 cut(s) 691
Cfr13I GGNCC 1 cut(s) 386
Cfr9I CCCGGG 1 cut(s) 144
Csp6I GTAC 3 cut(s) 321, 329, 496
CviAII CATG 1 cut(s) 692
CviQI GTAC 3 cut(s) 321, 329, 496
DdeI CTNAG 3 cut(s) 729, 744, 792
DpnI GATC 5 cut(s) 106, 177, 413, 690, 742
DpnII GATC 5 cut(s) 104, 175, 411, 688, 740
DraIII CACNNNGTG 1 cut(s) 596
Eam1104I CTCTTC 1 cut(s) 745
EarI CTCTTC 1 cut(s) 745
EciI GGCGGA 2 cut(s) 122, 200
Eco24I GRGCYC 1 cut(s) 757
Eco57I CTGAAG 2 cut(s) 243, 630
Eco88I CYCGRG 1 cut(s) 144
Eco91I GGTNACC 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 500
EcoRII CCWGG 3 cut(s) 231, 388, 736
EcoT22I ATGCAT 1 cut(s) 280
EcoT38I GRGCYC 1 cut(s) 757
Esp3I CGTCTC 1 cut(s) 352
FaeI CATG 1 cut(s) 695
FaiI YATR 7 cut(s) 280, 282, 287, 314, 482, 693, 806
FaqI GGGAC 1 cut(s) 350
FatI CATG 1 cut(s) 691
FbaI TGATCA 1 cut(s) 688
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 4 cut(s) 41, 123, 136, 237
FokI GGATG 1 cut(s) 698
FriOI GRGCYC 1 cut(s) 757
Fsp4HI GCNGC 4 cut(s) 41, 123, 136, 237
FspBI CTAG 4 cut(s) 401, 449, 513, 600
GluI GCNGC 4 cut(s) 41, 123, 136, 237
GsuI CTGGAG 1 cut(s) 765
HaeIII GGCC 3 cut(s) 387, 459, 511
HapII CCGG 1 cut(s) 145
Hin1II CATG 1 cut(s) 695
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 5 cut(s) 32, 95, 169, 247, 537
HpaII CCGG 1 cut(s) 145
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 3 cut(s) 331, 398, 541
Hpy188I TCNGA 1 cut(s) 795
Hpy188III TCNNGA 5 cut(s) 155, 173, 449, 608, 692
Hpy8I GTNNAC 3 cut(s) 331, 398, 541
Hpy99I CGWCG 1 cut(s) 81
HpyAV CCTTC 5 cut(s) 65, 449, 569, 619, 790
HpyCH4III ACNGT 5 cut(s) 47, 163, 335, 500, 698
HpyCH4IV ACGT 2 cut(s) 361, 579
HpyCH4V TGCA 6 cut(s) 43, 278, 377, 480, 635, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 637, 641, 797
HpyF3I CTNAG 3 cut(s) 729, 744, 792
HpySE526I ACGT 2 cut(s) 361, 579
Hsp92II CATG 1 cut(s) 695
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 5 cut(s) 104, 175, 411, 688, 740
LguI GCTCTTC 1 cut(s) 745
LmnI GCTCC 6 cut(s) 244, 300, 353, 491, 610, 628
Lsp1109I GCAGC 3 cut(s) 27, 109, 223
LweI GCATC 2 cut(s) 265, 776
MaeI CTAG 4 cut(s) 401, 449, 513, 600
MaeII ACGT 2 cut(s) 361, 579
MaeIII GTNAC 3 cut(s) 33, 500, 698
MalI GATC 5 cut(s) 106, 177, 413, 690, 742
MboI GATC 5 cut(s) 104, 175, 411, 688, 740
MboII GAAGA 3 cut(s) 178, 719, 762
MflI RGATCY 4 cut(s) 104, 175, 411, 740
MhlI GDGCHC 4 cut(s) 524, 607, 757, 821
MluCI AATT 1 cut(s) 483
MlyI GAGTC 3 cut(s) 41, 241, 546
MnlI CCTC 8 cut(s) 11, 44, 149, 220, 261, 573, 676, 772
Mph1103I ATGCAT 1 cut(s) 280
MseI TTAA 1 cut(s) 260
MslI CAYNNNNRTG 1 cut(s) 696
MspA1I CMGCKG 2 cut(s) 40, 122
MspI CCGG 1 cut(s) 145
MspR9I CCNGG 5 cut(s) 145, 146, 233, 390, 738
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 3 cut(s) 233, 390, 738
MvnI CGCG 1 cut(s) 594
MwoI GCNNNNNNNGC 4 cut(s) 233, 637, 641, 797
NciI CCSGG 2 cut(s) 145, 146
NdeII GATC 5 cut(s) 104, 175, 411, 688, 740
NlaIII CATG 1 cut(s) 695
NlaIV GGNNCC 5 cut(s) 106, 436, 521, 630, 818
NmuCI GTSAC 3 cut(s) 33, 500, 698
NsiI ATGCAT 1 cut(s) 280
PagI TCATGA 1 cut(s) 691
PciSI GCTCTTC 1 cut(s) 745
PcsI WCGNNNNNNNCGW 1 cut(s) 532
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 95, 169
Pfl23II CGTACG 1 cut(s) 495
PkrI GCNGC 4 cut(s) 42, 124, 137, 238
PleI GAGTC 3 cut(s) 40, 241, 545
PpsI GAGTC 3 cut(s) 40, 241, 545
Ppu21I YACGTR 1 cut(s) 580
Psp6I CCWGG 3 cut(s) 231, 388, 736
PspEI GGTNACC 1 cut(s) 500
PspGI CCWGG 3 cut(s) 231, 388, 736
PspLI CGTACG 1 cut(s) 495
PspN4I GGNNCC 5 cut(s) 106, 436, 521, 630, 818
PspPI GGNCC 1 cut(s) 386
PstNI CAGNNNCTG 1 cut(s) 788
PsuI RGATCY 4 cut(s) 104, 175, 411, 740
PvuII CAGCTG 2 cut(s) 40, 122
RsaI GTAC 3 cut(s) 322, 330, 497
RsaNI GTAC 3 cut(s) 321, 329, 496
RseI CAYNNNNRTG 1 cut(s) 696
SalI GTCGAC 1 cut(s) 539
SapI GCTCTTC 1 cut(s) 745
SaqAI TTAA 1 cut(s) 260
SatI GCNGC 4 cut(s) 41, 123, 136, 237
Sau3AI GATC 5 cut(s) 104, 175, 411, 688, 740
Sau96I GGNCC 1 cut(s) 386
SchI GAGTC 3 cut(s) 41, 241, 546
ScrFI CCNGG 5 cut(s) 145, 146, 233, 390, 738
SduI GDGCHC 4 cut(s) 524, 607, 757, 821
SfaNI GCATC 2 cut(s) 265, 776
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SpeI ACTAGT 1 cut(s) 400
Sse9I AATT 1 cut(s) 483
SsiI CCGC 5 cut(s) 26, 133, 136, 185, 594
SspMI CTAG 4 cut(s) 401, 449, 513, 600
StyD4I CCNGG 5 cut(s) 143, 144, 231, 388, 736
TaaI ACNGT 5 cut(s) 47, 163, 335, 500, 698
TaiI ACGT 2 cut(s) 364, 582
TaqI TCGA 2 cut(s) 535, 540
TasI AATT 1 cut(s) 483
TatI WGTACW 1 cut(s) 320
TauI GCSGC 1 cut(s) 138
TfiI GAWTC 2 cut(s) 95, 169
Tru1I TTAA 1 cut(s) 260
Tru9I TTAA 1 cut(s) 260
TscAI CASTG 2 cut(s) 512, 703
TseFI GTSAC 3 cut(s) 33, 500, 698
TseI GCWGC 3 cut(s) 40, 122, 236
Tsp45I GTSAC 3 cut(s) 33, 500, 698
TspDTI ATGAA 4 cut(s) 330, 468, 720, 729
TspGWI ACGGA 1 cut(s) 414
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 2 cut(s) 512, 703
XbaI TCTAGA 1 cut(s) 448
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 4 cut(s) 401, 449, 513, 600
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.