Rmu_sc0002516.1_g000013

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002516.1
OG0000181
Physical Location & Seq
Forward (+)
67621 .. 71042
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002516.1_g000013.1.cds

Sequence Viewer

Length: 1029 bp
atggagggtggtctgaaatccattcctcctactgaagatgtgcatatggaacctaaggtttacaagaagagaagcaggaatggaggctatgctttgggtttggtggaaactgacaaagacattgagcaaggcatcctgaagaagaaggttggcaggcctctggggagtaagaataagaagcctcgtcctcaaaagatggtgagtaggacttctatgtgtcttacttaccctactattgaaattgttcaagaatttagccctatagagaagggcaaagatgacgccatgtctcttgtcgaatcagaattctccaaggatccgaacccgaaccaggtcccgccggaggtggacgacaagatccagactcatggaaaaaacaaagctgctgaagactctctgtctcaacttgaagtagaggaggaggaggaagaggaagaggaagaagatgatgatgatgatgaagaagaagaagaagaatttgagatgtgtgatgtttgtttgaaggacaaggaacactggacggatgaatgcccatacctggctcttgaagtagaggaggaggaggaagaggaagaggaagaggaagaagatgatgatgatgatgatgaagaagaagcagaagaagaagaagaagaagaagaagaagaatttgagatgtgtgatgtttgtttgaaggacaaggaacactggacggatgaatgcccatacctggttcgtatcccaaacccaatggaagtaactcttggcaaaggttattatatggtttgtgtgcactgtaatctgaggggtggccactctgatatagttggtacacaatgtaaagtgtggggtgaccaccctgataggaggtggaaaggacgtgctattgtcaaggtctgtggtatttgtgagggcgtacgccactggtctgcagagtgcccaaaaaagcacttattacaaaagcaaaagagtctgttggagtgtgctgctgaatatcggtcgtggttggctaccaaaaaatttcaagctgctttatttgttgaatcataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

39.5

Weight (kDa)

4.42

Isoelectric Point (pI)

76.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000181)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02310 FvH4_1g02340 FvH4_1g02340 FvH4_1g02340 FvH4_3g13110 FvH4_4g04530 FvH4_5g26960 FvH4_5g26990 FvH4_6g01091 FvH4_6g08071 FvH4_6g19630 FvH4_6g38111 FvH4_6g38112 FvH4_6g48801 FvH4_6g48810 FvH4_6g48861 FvH4_6g49020 FvH4_6g49040 FvH4_6g49040 FvH4_6g49060 FvH4_6g49070 FvH4_6g49070 FvH4_6g49870 FvH4_6g49880
malus_domestica MD05G1007300.v1.1 MD05G1007600.v1.1 MD08G1121900.v1.1 MD08G1122000.v1.1 MD08G1122200.v1.1 MD08G1122700.v1.1
prunus_persica Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G019700_v2.0.a1 Prupe.1G187100_v2.0.a1 Prupe.2G227700_v2.0.a1 Prupe.2G227800_v2.0.a1 Prupe.2G227900_v2.0.a1 Prupe.4G043000_v2.0.a1 Prupe.4G046400_v2.0.a1 Prupe.4G077400_v2.0.a1 Prupe.4G077500_v2.0.a1 Prupe.4G159200_v2.0.a1 Prupe.4G159300_v2.0.a1 Prupe.4G159500_v2.0.a1 Prupe.6G311400_v2.0.a1 Prupe.6G311400_v2.0.a1
pyrus_communis pycom05g00390 pycom07g27750 pycom08g10150 pycom08g10170
rosa_chinensis RchiOBHm_Chr1g0328161 RchiOBHm_Chr1g0372961 RchiOBHm_Chr2g0131001 RchiOBHm_Chr2g0151211 RchiOBHm_Chr2g0151231 RchiOBHm_Chr2g0151281 RchiOBHm_Chr2g0151321 RchiOBHm_Chr2g0170041 RchiOBHm_Chr3g0457761 RchiOBHm_Chr3g0457871 RchiOBHm_Chr5g0014841 RchiOBHm_Chr5g0021371 RchiOBHm_Chr5g0038581 RchiOBHm_Chr5g0038691 RchiOBHm_Chr5g0038761 RchiOBHm_Chr6g0246131 RchiOBHm_Chr6g0246181
rosa_laevigata RLG00000015270 RLG00000020541 RLG00000021919 RLG00000025188 RLG00000032146 RLG00000032147 RLG00000032607 RLG00000033860
rosa_multiflora Rmu_sc0001102.1_g000003 Rmu_sc0002298.1_g000007 Rmu_sc0002516.1_g000005 Rmu_sc0002516.1_g000013 Rmu_sc0003629.1_g000050 Rmu_sc0023055.1_g000001
rosa_roxburghii Rroxscaffold_1G00042380 Rroxscaffold_1G00042450 Rroxscaffold_1G00042460 Rroxscaffold_1G00042550 Rroxscaffold_1G00061700 Rroxscaffold_1G00061730 Rroxscaffold_1G00063310 Rroxscaffold_1G00065700 Rroxscaffold_2G00096530 Rroxscaffold_2G00096560 Rroxscaffold_2G00096590 Rroxscaffold_2G00096660 Rroxscaffold_2G00101110 Rroxscaffold_2G00113390 Rroxscaffold_5G00339030 Rroxscaffold_5G00351680 Rroxscaffold_6G00421510 Rroxscaffold_7G00168250 Rroxscaffold_7G00214760 Rroxscaffold_7G00214840
rosa_rugosa Rorug02G0294400 Rorug02G0545100 Rorug05G0008000 Rorug05G0066600 Rorug05G0173700 Rorug05G0173800 Rorug05G0290700 Rorug05G0290800 Rorug05G0290800 Rorug05G0519600 Rorug05G0519700
rosa_samantha Rh1CG087500 Rh1DG094400 Rh2AG570900 Rh2AG612800 Rh2BG354000 Rh2BG498100 Rh2BG498500 Rh2BG498600 Rh2BG628900 Rh2DG372100 Rh2DG509000 Rh2DG509300 Rh2DG509400 Rh2DG509600 Rh2DG640700 Rh3AG083900 Rh3BG086400 Rh3CG086700 Rh5AG102700 Rh5AG259700 Rh5AG261200 Rh5BG098700 Rh5BG098800 Rh5BG099500 Rh5BG113500 Rh5BG113900 Rh5BG155300 Rh5BG264600 Rh5CG111100 Rh5CG125200 Rh5CG169000 Rh5CG297600 Rh5DG098300 Rh5DG272900 Rh6AG033700 Rh6BG028900 Rh6CG029100 Rh6CG029200 Rh6DG028200 Rh6DG028300 Rh7CG429700
rosa_wichuraiana Rw0G009330 Rw0G012230 Rw0G018980 Rw1G008830 Rw2G035660 Rw2G039830 Rw2G040230 Rw2G040240 Rw5G013700 Rw5G024330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 338
AclWI GGATC 3 cut(s) 311, 324, 352
AcoI YGGCCR 1 cut(s) 790
AcsI RAATTY 5 cut(s) 251, 305, 476, 647, 998
AcuI CTGAAG 3 cut(s) 54, 158, 408
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 811, 897
AfiI CCNNNNNNNGG 4 cut(s) 331, 343, 538, 709
AgsI TTSAA 8 cut(s) 239, 248, 410, 502, 548, 673, 1004, 1022
AhdI GACNNNNNGTC 2 cut(s) 286, 397
AjiI CACGTC 1 cut(s) 860
AjnI CCWGG 3 cut(s) 330, 537, 708
AjuI GAANNNNNNNTTGG 2 cut(s) 726, 758
AluBI AGCT 2 cut(s) 383, 1007
AluI AGCT 2 cut(s) 383, 1007
Alw21I GWGCWC 1 cut(s) 774
Alw26I GTCTC 2 cut(s) 294, 405
Alw44I GTGCAC 1 cut(s) 770
AlwI GGATC 3 cut(s) 311, 324, 352
AoxI GGCC 2 cut(s) 155, 790
ApaLI GTGCAC 1 cut(s) 770
ApeKI GCWGC 3 cut(s) 383, 965, 1007
ApoI RAATTY 5 cut(s) 251, 305, 476, 647, 998
Asp700I GAANNNNTTC 1 cut(s) 243
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 211, 842
AvaII GGWCC 1 cut(s) 334
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 2 cut(s) 774, 920
BalI TGGCCA 1 cut(s) 792
BamHI GGATCC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 774
BbvI GCAGC 3 cut(s) 370, 952, 994
BccI CCATC 1 cut(s) 190
BciT130I CCWGG 3 cut(s) 332, 539, 710
BciVI GTATCC 1 cut(s) 728
BcoDI GTCTC 2 cut(s) 294, 405
BfmI CTRYAG 2 cut(s) 261, 909
BfuI GTATCC 1 cut(s) 728
BisI GCNGC 3 cut(s) 384, 966, 1008
BlsI GCNGC 3 cut(s) 385, 967, 1009
Bme1390I CCNGG 3 cut(s) 332, 539, 710
Bme18I GGWCC 1 cut(s) 334
BmeRI GACNNNNNGTC 2 cut(s) 286, 397
BmgBI CACGTC 1 cut(s) 860
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 3 cut(s) 51, 318, 336
BmrFI CCNGG 3 cut(s) 332, 539, 710
BmsI GCATC 1 cut(s) 141
BpiI GAAGAC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 1 cut(s) 312
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 343, 538, 709
Bse1I ACTGG 3 cut(s) 521, 692, 908
Bse21I CCTNAGG 1 cut(s) 54
BseBI CCWGG 3 cut(s) 332, 539, 710
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 3 cut(s) 132, 529, 700
BseLI CCNNNNNNNGG 4 cut(s) 331, 343, 538, 709
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 3 cut(s) 521, 692, 908
BseRI GAGGAG 6 cut(s) 431, 434, 437, 569, 572, 575
BseSI GKGCMC 2 cut(s) 774, 920
BseXI GCAGC 3 cut(s) 370, 952, 994
Bsh1285I CGRYCG 1 cut(s) 980
BshFI GGCC 2 cut(s) 157, 792
BsiEI CGRYCG 1 cut(s) 980
BsiHKAI GWGCWC 1 cut(s) 774
BsiSI CCGG 1 cut(s) 341
BsiWI CGTACG 1 cut(s) 895
BslFI GGGAC 1 cut(s) 320
BslI CCNNNNNNNGG 4 cut(s) 331, 343, 538, 709
BsmAI GTCTC 2 cut(s) 294, 405
BsmFI GGGAC 1 cut(s) 320
BsmI GAATGC 2 cut(s) 533, 704
BsnI GGCC 2 cut(s) 157, 792
Bsp1286I GDGCHC 2 cut(s) 774, 920
Bsp143I GATC 2 cut(s) 316, 357
BspACI CCGC 1 cut(s) 338
BspANI GGCC 2 cut(s) 157, 792
BspCNI CTCAG 1 cut(s) 774
BspLI GGNNCC 3 cut(s) 51, 318, 336
BspMAI CTGCAG 1 cut(s) 913
BspPI GGATC 3 cut(s) 311, 324, 352
BsrI ACTGG 3 cut(s) 521, 692, 908
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 2 cut(s) 316, 357
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 312
Bst2UI CCWGG 3 cut(s) 332, 539, 710
Bst4CI ACNGT 1 cut(s) 776
Bst6I CTCTTC 6 cut(s) 62, 423, 429, 561, 567, 573
BstACI GRCGYC 1 cut(s) 282
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 2 cut(s) 54, 782
BstEII GGTNACC 1 cut(s) 830
BstF5I GGATG 3 cut(s) 132, 529, 700
BstKTI GATC 2 cut(s) 319, 360
BstMAI GTCTC 2 cut(s) 294, 405
BstMBI GATC 2 cut(s) 316, 357
BstMCI CGRYCG 1 cut(s) 980
BstNI CCWGG 3 cut(s) 332, 539, 710
BstPI GGTNACC 1 cut(s) 830
BstSCI CCNGG 3 cut(s) 330, 537, 708
BstSFI CTRYAG 2 cut(s) 261, 909
BstSLI GKGCMC 2 cut(s) 774, 920
BstV1I GCAGC 3 cut(s) 370, 952, 994
BstV2I GAAGAC 1 cut(s) 396
BstX2I RGATCY 2 cut(s) 316, 357
BstXI CCANNNNNNTGG 1 cut(s) 368
BstYI RGATCY 2 cut(s) 316, 357
Bsu36I CCTNAGG 1 cut(s) 54
BsuI GTATCC 1 cut(s) 728
BsuRI GGCC 2 cut(s) 157, 792
BtrI CACGTC 1 cut(s) 860
BtsCI GGATG 3 cut(s) 132, 529, 700
BtsIMutI CAGTG 4 cut(s) 514, 685, 772, 901
Cac8I GCNNGC 1 cut(s) 155
Cfr13I GGNCC 1 cut(s) 334
CseI GACGC 1 cut(s) 290
CsiI ACCWGGT 2 cut(s) 330, 708
Csp6I GTAC 2 cut(s) 810, 896
CspCI CAANNNNNGTGG 2 cut(s) 859, 894
CviAII CATG 2 cut(s) 286, 368
CviJI RGCY 9 cut(s) 87, 157, 181, 258, 383, 542, 792, 989, 1007
CviKI_1 RGCY 9 cut(s) 87, 157, 181, 258, 383, 542, 792, 989, 1007
CviQI GTAC 2 cut(s) 810, 896
DdeI CTNAG 2 cut(s) 54, 782
DpnI GATC 2 cut(s) 318, 359
DpnII GATC 2 cut(s) 316, 357
DriI GACNNNNNGTC 2 cut(s) 286, 397
EaeI YGGCCR 1 cut(s) 790
Eam1104I CTCTTC 6 cut(s) 62, 423, 429, 561, 567, 573
Eam1105I GACNNNNNGTC 2 cut(s) 286, 397
EarI CTCTTC 6 cut(s) 62, 423, 429, 561, 567, 573
Eco130I CCWWGG 1 cut(s) 312
Eco147I AGGCCT 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 334
Eco57I CTGAAG 3 cut(s) 54, 158, 408
Eco81I CCTNAGG 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 830
EcoO109I RGGNCCY 1 cut(s) 334
EcoO65I GGTNACC 1 cut(s) 830
EcoRI GAATTC 1 cut(s) 305
EcoRII CCWGG 3 cut(s) 330, 537, 708
EcoT14I CCWWGG 1 cut(s) 312
ErhI CCWWGG 1 cut(s) 312
FaeI CATG 2 cut(s) 289, 371
FalI AAGNNNNNCTT 2 cut(s) 726, 758
FaqI GGGAC 1 cut(s) 320
FatI CATG 2 cut(s) 285, 367
FauI CCCGC 1 cut(s) 345
FauNDI CATATG 1 cut(s) 45
Fnu4HI GCNGC 3 cut(s) 384, 966, 1008
FokI GGATG 3 cut(s) 119, 536, 707
Fsp4HI GCNGC 3 cut(s) 384, 966, 1008
GluI GCNGC 3 cut(s) 384, 966, 1008
HaeIII GGCC 2 cut(s) 157, 792
HapII CCGG 1 cut(s) 341
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 2 cut(s) 289, 371
HinfI GANTC 5 cut(s) 299, 364, 392, 949, 1022
HpaII CCGG 1 cut(s) 341
HphI GGTGA 2 cut(s) 211, 842
Hpy166II GTNNAC 4 cut(s) 61, 349, 772, 812
Hpy188I TCNGA 5 cut(s) 15, 304, 321, 783, 799
Hpy188III TCNNGA 4 cut(s) 136, 248, 361, 545
Hpy8I GTNNAC 4 cut(s) 61, 349, 772, 812
HpyAV CCTTC 4 cut(s) 139, 262, 496, 667
HpyCH4III ACNGT 1 cut(s) 776
HpyCH4IV ACGT 1 cut(s) 859
HpyCH4V TGCA 3 cut(s) 43, 772, 911
HpyF3I CTNAG 2 cut(s) 54, 782
HpySE526I ACGT 1 cut(s) 859
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 2 cut(s) 289, 371
Kzo9I GATC 2 cut(s) 316, 357
Lsp1109I GCAGC 3 cut(s) 370, 952, 994
LweI GCATC 1 cut(s) 141
MabI ACCWGGT 2 cut(s) 330, 708
MaeII ACGT 1 cut(s) 859
MaeIII GTNAC 2 cut(s) 736, 830
MalI GATC 2 cut(s) 318, 359
MboI GATC 2 cut(s) 316, 357
MflI RGATCY 2 cut(s) 316, 357
MhlI GDGCHC 2 cut(s) 774, 920
MlsI TGGCCA 1 cut(s) 792
MluCI AATT 6 cut(s) 240, 251, 305, 476, 647, 998
MluNI TGGCCA 1 cut(s) 792
MlyI GAGTC 3 cut(s) 358, 386, 958
MmeI TCCRAC 1 cut(s) 936
Mox20I TGGCCA 1 cut(s) 792
MroXI GAANNNNTTC 1 cut(s) 243
MscI TGGCCA 1 cut(s) 792
Msp20I TGGCCA 1 cut(s) 792
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 3 cut(s) 332, 539, 710
Mva1269I GAATGC 2 cut(s) 533, 704
MvaI CCWGG 3 cut(s) 332, 539, 710
NdeI CATATG 1 cut(s) 45
NdeII GATC 2 cut(s) 316, 357
NlaIII CATG 2 cut(s) 289, 371
NlaIV GGNNCC 3 cut(s) 51, 318, 336
NmuCI GTSAC 1 cut(s) 830
PceI AGGCCT 1 cut(s) 157
PctI GAATGC 2 cut(s) 533, 704
PdmI GAANNNNTTC 1 cut(s) 243
PfeI GAWTC 2 cut(s) 299, 1022
Pfl23II CGTACG 1 cut(s) 895
PkrI GCNGC 3 cut(s) 385, 967, 1009
PleI GAGTC 3 cut(s) 358, 386, 957
PpsI GAGTC 3 cut(s) 358, 386, 957
PpuMI RGGWCCY 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 334
Psp6I CCWGG 3 cut(s) 330, 537, 708
PspEI GGTNACC 1 cut(s) 830
PspGI CCWGG 3 cut(s) 330, 537, 708
PspLI CGTACG 1 cut(s) 895
PspN4I GGNNCC 3 cut(s) 51, 318, 336
PspPI GGNCC 1 cut(s) 334
PspPPI RGGWCCY 1 cut(s) 334
PstI CTGCAG 1 cut(s) 913
PsuI RGATCY 2 cut(s) 316, 357
RsaI GTAC 2 cut(s) 811, 897
RsaNI GTAC 2 cut(s) 810, 896
SatI GCNGC 3 cut(s) 384, 966, 1008
Sau3AI GATC 2 cut(s) 316, 357
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 3 cut(s) 358, 386, 958
ScrFI CCNGG 3 cut(s) 332, 539, 710
SduI GDGCHC 2 cut(s) 774, 920
SexAI ACCWGGT 2 cut(s) 330, 708
SfaNI GCATC 1 cut(s) 141
SfcI CTRYAG 2 cut(s) 261, 909
SinI GGWCC 1 cut(s) 334
Sse9I AATT 6 cut(s) 240, 251, 305, 476, 647, 998
SseBI AGGCCT 1 cut(s) 157
SsiI CCGC 1 cut(s) 338
StuI AGGCCT 1 cut(s) 157
StyD4I CCNGG 3 cut(s) 330, 537, 708
StyI CCWWGG 1 cut(s) 312
TaaI ACNGT 1 cut(s) 776
TaiI ACGT 1 cut(s) 862
TaqI TCGA 1 cut(s) 297
TaqII GACCGA 1 cut(s) 966
TasI AATT 6 cut(s) 240, 251, 305, 476, 647, 998
TfiI GAWTC 2 cut(s) 299, 1022
TscAI CASTG 4 cut(s) 521, 692, 779, 908
TseFI GTSAC 1 cut(s) 830
TseI GCWGC 3 cut(s) 383, 965, 1007
Tsp45I GTSAC 1 cut(s) 830
TspDTI ATGAA 4 cut(s) 474, 540, 621, 711
TspGWI ACGGA 2 cut(s) 536, 707
TspRI CASTG 4 cut(s) 521, 692, 779, 908
VneI GTGCAC 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 5 cut(s) 251, 305, 476, 647, 998
XmnI GAANNNNTTC 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.