RchiOBHm_Chr2g0109251

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000169
Physical Location & Seq
Reverse (-)
20673195 .. 20674276
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48309

Sequence Viewer

Length: 921 bp
ATGAAACATGTTCATATCTGGGTTAGGGTTTCGGGCATTCCGCCTCTATATGAGGAACCTGATAATTTTACTCTCATTGGAAACCTTTTGGGAGGTTTTCTAGATTATGACAAGAAAGAATTATGGAAAGGAGTTATTCGCATATTCTTTTTGCATGATATCTCCAAACCCATTCTCTTTGAACGCATAATTTTCTTGGTGATAGGAGTTGAGCCTTTGTTGAAATTTCAGTTTGAATACCTCAAGGGTAAATGCTCTCAATGTGGCTTGATTACTCATTCTGGGGCCAAGTGTGAGGACCCTAATGCGGCAATCTCTACTCCTAGGGTATTGCACTTCAGCGGCTCCTCTACTACTGGTGGCTCTTTCTCCTTCTCAGCCCAAGCTAGTCGTGATTTACCTTTATCTTCTCCTGCTTTAATAAAGAAAAAGAAGCATGTCATTAGAAGAAAGGAAGGATCGGTTTCTGGGTTTGATTCTTCTGCTGGTACCGTGCAAAGTATGGCTGAGGAGGAGATATTACAGCCAATGAAGAATATGGATGAGGTGGTGGTCTCGAGATCGGCCTTCATGGAGGGTGTTCTGAAATCCATTCCTCCTACTGAAGATGTGCAGGTGGAACCTAAGGTGTACAGGAAGAAAGGCAGGAATGGAGGTTGTACCCCCTCCCCAAAGAACTTTAAAACTATCCTGGGGGGTAAGCTTCTTACTCTTCAAGCAGATACTTTGGGTTTGGTGGAAACTGACAAAGAGAGTGAGCAAGGCATCTTGAAGAAGAAGGTTGGCAGGCCTCTAGGGCGTAAGAATAAGAATCCTTGTCCTCAGAAGATGGTGAGTAGGATGCATCTGCGTCTTACTTACCCTATTATGGAAACTGGTCAAGAGAGTAGCCCTATAGAGAAGGGCAAAGGTAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

306

Amino Acids

33.91

Weight (kDa)

9.52

Isoelectric Point (pI)

44.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 53 - 99 1.3e-08 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000169)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13681 FvH4_3g25832 FvH4_3g26062 FvH4_4g08041 FvH4_4g13361 FvH4_4g16721 FvH4_4g21881 FvH4_6g07632 FvH4_6g17062 FvH4_6g26370 FvH4_7g07641
rosa_chinensis RchiOBHm_Chr1g0320861 RchiOBHm_Chr2g0109251 RchiOBHm_Chr3g0472781 RchiOBHm_Chr6g0288921 RchiOBHm_Chr7g0191711 RchiOBHm_Chr7g0238801
rosa_laevigata RLG00000007550 RLG00000036731
rosa_multiflora Rmu_co8018808.1_g000001 Rmu_co8401665.1_g000001 Rmu_co8461051.1_g000001 Rmu_sc0000144.1_g000042 Rmu_sc0000144.1_g000051 Rmu_sc0000160.1_g000018 Rmu_sc0000245.1_g000011 Rmu_sc0000299.1_g000021 Rmu_sc0000348.1_g000003 Rmu_sc0000367.1_g000120 Rmu_sc0000424.1_g000037 Rmu_sc0000427.1_g000006 Rmu_sc0000427.1_g000023 Rmu_sc0000427.1_g000037 Rmu_sc0000604.1_g000005 Rmu_sc0000605.1_g000060 Rmu_sc0000621.1_g000030 Rmu_sc0000693.1_g000017 Rmu_sc0000708.1_g000012 Rmu_sc0000918.1_g000031 Rmu_sc0000953.1_g000027 Rmu_sc0001030.1_g000008 Rmu_sc0001030.1_g000011 Rmu_sc0001051.1_g000026 Rmu_sc0001055.1_g000001 Rmu_sc0001081.1_g000001 Rmu_sc0001175.1_g000002 Rmu_sc0001176.1_g000026 Rmu_sc0001181.1_g000010 Rmu_sc0001225.1_g000003 Rmu_sc0001371.1_g000008 Rmu_sc0001433.1_g000012 Rmu_sc0001507.1_g000009 Rmu_sc0001643.1_g000014 Rmu_sc0001879.1_g000002 Rmu_sc0001890.1_g000006 Rmu_sc0002129.1_g000039 Rmu_sc0002356.1_g000008 Rmu_sc0002358.1_g000003 Rmu_sc0002553.1_g000024 Rmu_sc0002826.1_g000007 Rmu_sc0002872.1_g000007 Rmu_sc0003323.1_g000026 Rmu_sc0003410.1_g000001 Rmu_sc0003511.1_g000042 Rmu_sc0003541.1_g000036 Rmu_sc0003605.1_g000048 Rmu_sc0003689.1_g000014 Rmu_sc0003692.1_g000010 Rmu_sc0003720.1_g000001 Rmu_sc0003813.1_g000003 Rmu_sc0003872.1_g000005 Rmu_sc0004010.1_g000012 Rmu_sc0004165.1_g000092 Rmu_sc0004230.1_g000013 Rmu_sc0004318.1_g000020 Rmu_sc0004353.1_g000004 Rmu_sc0004617.1_g000005 Rmu_sc0004617.1_g000006 Rmu_sc0004658.1_g000005 Rmu_sc0005356.1_g000008 Rmu_sc0005994.1_g000014 Rmu_sc0006388.1_g000004 Rmu_sc0006674.1_g000020 Rmu_sc0006898.1_g000008 Rmu_sc0007286.1_g000005 Rmu_sc0007476.1_g000002 Rmu_sc0007671.1_g000005 Rmu_sc0007991.1_g000008 Rmu_sc0008131.1_g000008 Rmu_sc0008163.1_g000011 Rmu_sc0008322.1_g000004 Rmu_sc0008334.1_g000017 Rmu_sc0008835.1_g000009 Rmu_sc0008835.1_g000011 Rmu_sc0009954.1_g000006 Rmu_sc0010050.1_g000001 Rmu_sc0010317.1_g000011 Rmu_sc0010426.1_g000002 Rmu_sc0011111.1_g000005 Rmu_sc0014614.1_g000003 Rmu_sc0014688.1_g000002 Rmu_sc0015167.1_g000003 Rmu_sc0015936.1_g000003 Rmu_sc0017323.1_g000003 Rmu_sc0018541.1_g000001 Rmu_sc0019370.1_g000003 Rmu_sc0020444.1_g000001 Rmu_sc0020600.1_g000003 Rmu_sc0021238.1_g000001 Rmu_sc0022909.1_g000001 Rmu_sc0030397.1_g000001 Rmu_sc0030484.1_g000002 Rmu_sc0035139.1_g000001 Rmu_sc0035719.1_g000001 Rmu_ssc0000287.1_g000010 Rmu_ssc0000451.1_g000005
rosa_roxburghii Rroxscaffold_1G00046590 Rroxscaffold_1G00050100 Rroxscaffold_2G00079390 Rroxscaffold_2G00088790 Rroxscaffold_2G00097170 Rroxscaffold_2G00126350 Rroxscaffold_3G00221550 Rroxscaffold_3G00222700 Rroxscaffold_3G00236390 Rroxscaffold_3G00254000 Rroxscaffold_3G00260090 Rroxscaffold_3G00263600 Rroxscaffold_5G00336900
rosa_rugosa Rorug01G0048200 Rorug01G0172000 Rorug01G0294000 Rorug01G0308200 Rorug01G0355300 Rorug02G0000900 Rorug02G0129600 Rorug02G0136800 Rorug02G0179700 Rorug02G0251500 Rorug02G0305800 Rorug02G0305900 Rorug02G0373800 Rorug02G0396100 Rorug02G0411300 Rorug02G0411400 Rorug02G0411500 Rorug02G0460200 Rorug03G0187400 Rorug03G0299000 Rorug04G0041700 Rorug04G0130800 Rorug04G0168300 Rorug04G0198700 Rorug05G0095100 Rorug05G0194100 Rorug05G0205300 Rorug05G0235300 Rorug06G0088100.1 Rorug06G0120100 Rorug06G0164300 Rorug07G0163600 Rorug07G0163700 Rorug07G0236200 Rorug07G0307200 RorugPtG0006100
rosa_samantha Rh1DG036900 Rh3BG176200 Rh4CG455100 Rh6AG142400 Rh6AG263000 Rh6CG138900 Rh6DG126200 Rh7CG393300 Rh7CG447500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 604
Acc36I ACCTGC 1 cut(s) 604
Acc65I GGTACC 1 cut(s) 488
AccB1I GGYRCC 1 cut(s) 488
AciI CCGC 3 cut(s) 41, 308, 342
AclWI GGATC 1 cut(s) 466
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 2 cut(s) 322, 624
AfaI GTAC 3 cut(s) 490, 632, 661
AfiI CCNNNNNNNGG 2 cut(s) 307, 868
AflIII ACRYGT 1 cut(s) 7
AgsI TTSAA 5 cut(s) 182, 223, 236, 716, 772
AjnI CCWGG 1 cut(s) 690
AluBI AGCT 3 cut(s) 386, 703, 916
AluI AGCT 3 cut(s) 386, 703, 916
Alw26I GTCTC 1 cut(s) 559
AlwI GGATC 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 556
AoxI GGCC 3 cut(s) 285, 564, 788
ApoI RAATTY 1 cut(s) 224
Asp718I GGTACC 1 cut(s) 488
AspA2I CCTAGG 1 cut(s) 323
AspS9I GGNCC 2 cut(s) 285, 298
AsuHPI GGTGA 2 cut(s) 211, 844
AvaI CYCGRG 1 cut(s) 556
AvaII GGWCC 1 cut(s) 298
AvrII CCTAGG 1 cut(s) 323
AxyI CCTNAGG 1 cut(s) 624
BanI GGYRCC 1 cut(s) 488
BbvCI CCTCAGC 1 cut(s) 507
BccI CCATC 1 cut(s) 823
BciT130I CCWGG 1 cut(s) 692
BcoDI GTCTC 1 cut(s) 559
BfaI CTAG 4 cut(s) 101, 324, 387, 794
BfmI CTRYAG 1 cut(s) 894
BfuAI ACCTGC 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 796
BisI GCNGC 2 cut(s) 309, 343
BlnI CCTAGG 1 cut(s) 323
BlsI GCNGC 2 cut(s) 310, 344
Bme1390I CCNGG 1 cut(s) 692
Bme18I GGWCC 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 556
BmgT120I GGNCC 2 cut(s) 285, 298
BmiI GGNNCC 6 cut(s) 57, 286, 300, 346, 490, 621
BmrFI CCNGG 1 cut(s) 692
BmsI GCATC 3 cut(s) 774, 831, 853
Bpu10I CCTNAGC 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 227
BsaI GGTCTC 1 cut(s) 559
BsaJI CCNNGG 2 cut(s) 323, 691
Bsc4I CCNNNNNNNGG 2 cut(s) 307, 868
Bse1I ACTGG 2 cut(s) 361, 880
Bse21I CCTNAGG 1 cut(s) 624
BseBI CCWGG 1 cut(s) 692
BseDI CCNNGG 2 cut(s) 323, 691
BseGI GGATG 2 cut(s) 547, 846
BseLI CCNNNNNNNGG 2 cut(s) 307, 868
BseMII CTCAG 3 cut(s) 390, 498, 836
BseNI ACTGG 2 cut(s) 361, 880
BseRI GAGGAG 3 cut(s) 337, 524, 527
BsgI GTGCAG 1 cut(s) 632
BshFI GGCC 3 cut(s) 287, 566, 790
BshNI GGYRCC 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 556
BslI CCNNNNNNNGG 2 cut(s) 307, 868
BsmAI GTCTC 1 cut(s) 559
BsmI GAATGC 1 cut(s) 36
BsnI GGCC 3 cut(s) 287, 566, 790
Bso31I GGTCTC 1 cut(s) 559
BsoBI CYCGRG 1 cut(s) 556
Bsp1407I TGTACA 1 cut(s) 630
Bsp143I GATC 2 cut(s) 458, 560
BspACI CCGC 3 cut(s) 41, 308, 342
BspANI GGCC 3 cut(s) 287, 566, 790
BspCNI CTCAG 3 cut(s) 389, 499, 835
BspLI GGNNCC 6 cut(s) 57, 286, 300, 346, 490, 621
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 1 cut(s) 466
BspT107I GGYRCC 1 cut(s) 488
BspTNI GGTCTC 1 cut(s) 559
BsrGI TGTACA 1 cut(s) 630
BsrI ACTGG 2 cut(s) 361, 880
BssECI CCNNGG 2 cut(s) 323, 691
BssMI GATC 2 cut(s) 458, 560
BssT1I CCWWGG 1 cut(s) 323
Bst2UI CCWGG 1 cut(s) 692
Bst4CI ACNGT 1 cut(s) 493
Bst6I CTCTTC 1 cut(s) 717
BstAUI TGTACA 1 cut(s) 630
BstC8I GCNNGC 1 cut(s) 788
BstDEI CTNAG 4 cut(s) 376, 507, 624, 822
BstF5I GGATG 2 cut(s) 547, 846
BstKTI GATC 2 cut(s) 461, 563
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 2 cut(s) 458, 560
BstMWI GCNNNNNNNGC 1 cut(s) 796
BstNI CCWGG 1 cut(s) 692
BstNSI RCATGY 2 cut(s) 11, 440
BstSCI CCNGG 1 cut(s) 690
BstSFI CTRYAG 1 cut(s) 894
Bsu36I CCTNAGG 1 cut(s) 624
BsuRI GGCC 3 cut(s) 287, 566, 790
BtsCI GGATG 2 cut(s) 547, 846
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 1 cut(s) 788
Cfr13I GGNCC 2 cut(s) 285, 298
CseI GACGC 1 cut(s) 839
Csp6I GTAC 3 cut(s) 489, 631, 660
CviAII CATG 4 cut(s) 8, 155, 437, 571
CviQI GTAC 3 cut(s) 489, 631, 660
DdeI CTNAG 4 cut(s) 376, 507, 624, 822
DpnI GATC 2 cut(s) 460, 562
DpnII GATC 2 cut(s) 458, 560
DraI TTTAAA 1 cut(s) 682
Eam1104I CTCTTC 1 cut(s) 717
EarI CTCTTC 1 cut(s) 717
EciI GGCGGA 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 323
Eco147I AGGCCT 1 cut(s) 790
Eco31I GGTCTC 1 cut(s) 559
Eco32I GATATC 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 298
Eco57I CTGAAG 2 cut(s) 322, 624
Eco81I CCTNAGG 1 cut(s) 624
Eco88I CYCGRG 1 cut(s) 556
EcoO109I RGGNCCY 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 690
EcoRV GATATC 1 cut(s) 160
EcoT14I CCWWGG 1 cut(s) 323
EcoT22I ATGCAT 1 cut(s) 846
ErhI CCWWGG 1 cut(s) 323
FaeI CATG 4 cut(s) 11, 158, 440, 574
FatI CATG 4 cut(s) 7, 154, 436, 570
Fnu4HI GCNGC 2 cut(s) 309, 343
FokI GGATG 2 cut(s) 554, 853
Fsp4HI GCNGC 2 cut(s) 309, 343
FspBI CTAG 4 cut(s) 101, 324, 387, 794
GluI GCNGC 2 cut(s) 309, 343
HaeIII GGCC 3 cut(s) 287, 566, 790
HgaI GACGC 1 cut(s) 839
Hin1II CATG 4 cut(s) 11, 158, 440, 574
HindIII AAGCTT 1 cut(s) 701
HinfI GANTC 2 cut(s) 476, 811
HphI GGTGA 2 cut(s) 211, 844
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 2 cut(s) 585, 825
Hpy188III TCNNGA 6 cut(s) 101, 392, 556, 558, 769, 881
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 5 cut(s) 382, 449, 577, 772, 895
HpyCH4III ACNGT 1 cut(s) 493
HpyCH4V TGCA 5 cut(s) 154, 334, 496, 613, 844
HpyF10VI GCNNNNNNNGC 1 cut(s) 796
HpyF3I CTNAG 4 cut(s) 376, 507, 624, 822
Hsp92II CATG 4 cut(s) 11, 158, 440, 574
KpnI GGTACC 1 cut(s) 492
Kzo9I GATC 2 cut(s) 458, 560
LmnI GCTCC 1 cut(s) 350
LweI GCATC 3 cut(s) 774, 831, 853
MaeI CTAG 4 cut(s) 101, 324, 387, 794
MalI GATC 2 cut(s) 460, 562
MboI GATC 2 cut(s) 458, 560
MluCI AATT 4 cut(s) 64, 119, 189, 224
Mph1103I ATGCAT 1 cut(s) 846
MseI TTAA 2 cut(s) 419, 681
MspA1I CMGCKG 1 cut(s) 342
MspR9I CCNGG 1 cut(s) 692
Mva1269I GAATGC 1 cut(s) 36
MvaI CCWGG 1 cut(s) 692
MwoI GCNNNNNNNGC 1 cut(s) 796
NdeII GATC 2 cut(s) 458, 560
NlaIII CATG 4 cut(s) 11, 158, 440, 574
NlaIV GGNNCC 6 cut(s) 57, 286, 300, 346, 490, 621
NsiI ATGCAT 1 cut(s) 846
NspI RCATGY 2 cut(s) 11, 440
PaeR7I CTCGAG 1 cut(s) 556
PaqCI CACCTGC 1 cut(s) 604
PceI AGGCCT 1 cut(s) 790
PciI ACATGT 1 cut(s) 7
PctI GAATGC 1 cut(s) 36
PfeI GAWTC 2 cut(s) 476, 811
PkrI GCNGC 2 cut(s) 310, 344
PpuMI RGGWCCY 1 cut(s) 298
PscI ACATGT 1 cut(s) 7
Psp5II RGGWCCY 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 690
PspGI CCWGG 1 cut(s) 690
PspN4I GGNNCC 6 cut(s) 57, 286, 300, 346, 490, 621
PspPI GGNCC 2 cut(s) 285, 298
PspPPI RGGWCCY 1 cut(s) 298
RsaI GTAC 3 cut(s) 490, 632, 661
RsaNI GTAC 3 cut(s) 489, 631, 660
SaqAI TTAA 2 cut(s) 419, 681
SatI GCNGC 2 cut(s) 309, 343
Sau3AI GATC 2 cut(s) 458, 560
Sau96I GGNCC 2 cut(s) 285, 298
ScrFI CCNGG 1 cut(s) 692
SfaNI GCATC 3 cut(s) 774, 831, 853
SfcI CTRYAG 1 cut(s) 894
Sfr274I CTCGAG 1 cut(s) 556
SinI GGWCC 1 cut(s) 298
SlaI CTCGAG 1 cut(s) 556
SmlI CTYRAG 2 cut(s) 242, 556
SmoI CTYRAG 2 cut(s) 242, 556
Sse9I AATT 4 cut(s) 64, 119, 189, 224
SseBI AGGCCT 1 cut(s) 790
SsiI CCGC 3 cut(s) 41, 308, 342
SspMI CTAG 4 cut(s) 101, 324, 387, 794
StuI AGGCCT 1 cut(s) 790
StyD4I CCNGG 1 cut(s) 690
StyI CCWWGG 1 cut(s) 323
TaaI ACNGT 1 cut(s) 493
TaqI TCGA 1 cut(s) 557
TasI AATT 4 cut(s) 64, 119, 189, 224
TatI WGTACW 1 cut(s) 630
TauI GCSGC 2 cut(s) 311, 345
TfiI GAWTC 2 cut(s) 476, 811
Tru1I TTAA 2 cut(s) 419, 681
Tru9I TTAA 2 cut(s) 419, 681
TspDTI ATGAA 3 cut(s) 17, 545, 559
VpaK11BI GGWCC 1 cut(s) 298
XapI RAATTY 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 100
XceI RCATGY 2 cut(s) 11, 440
XhoI CTCGAG 1 cut(s) 556
XmaJI CCTAGG 1 cut(s) 323
XspI CTAG 4 cut(s) 101, 324, 387, 794
Zsp2I ATGCAT 1 cut(s) 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.