RchiOBHm_Chr3g0472781

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000169
Physical Location & Seq
Forward (+)
18766379 .. 18768515
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43847

Sequence Viewer

Length: 1260 bp
ATGGTGGATCGCGAGTTTAGGGTTCAGGATAAACCCGGAGATATTTTCCTGTTCTCTTTTGACTCGATGCATGACCGCAACAAGATACTCCGAGGTGGGGTTTGGTGCTTTGATCGAGCCCCAGTTTTCTTTGTAGAATATGATGGTGGCAAGCCTCTCCAGGAGATCCAGTTCCAACATATCCGTATTTGGGTTAGGGTTTCTGGAATTCCCCCTTTGTATGAAGAACCAGAAAATTTTACTCTCGTTGGTAATCTCTTAGGCGGCTTTTTGGATTTTGATAAGAAGGAGTTTAAGAAAGGAGTCATCCGGATCTTGATCTCCCATGACATCTCCAAGCCAGTCCTGCTAGAGCGACAAGTTTTTTTGGATAAGGGCGTGGAACCGACACTACACTTTCAGTTTGAACATCTCAAAGGCAGGTGCTCACAGTGTGGGCTCTTCACACACGCTGGGATCTCCTGTGAGATGCCGAATGTTGCTGTCATAACGCCTAGGGCTTCTTACTTTCCGGGCTCATCATCTTCGGGTGGTTCCTTTTCTTTTACAGACCAGAACAAGAGGGACTTCTTCACCGCGGCCTCCACTATCCCGAAGAAAAAGAAGCCCACGATCAGAAGACTCGACAGCTCTGTTAATATCTCCCCCACGCCGGCTGAATCACTGCTAGTTTCGGACTCAGTTGATGGAGTTCCTGGTGCTTCGGCGATGGCGCATGAACTGGAACAGAATAAAGTTTTAGATGGGAATGAGCATACTGGTGCTGGTTTGGGACAAGCTAATGGTGGGGATATTGACCGTGTGCAGGTGGTCCAGGAGGATATCTTGGGGTCTAAGAAAAGGGAGCGCGCTGATGTTTGCAATCCCTCCCCGAAAAAGTTTAAGACCATACTCGGCCGCAAAATTCCAACGTTGCAGGCTAATGCTCTGGGATTGAGGGATATGGACAGTGATGTTCCACTGGTTACTCTGAAGAAGAAGTTGGGTCGTCCCTTGGGCAGTAGGAACAAGCAGCACCGGTCTGTTTGGCCACAGCTTGCTTCAAGAAGAGGTTATGGGACCAGGACTCTTTTGAGGAGAGTGAAAGTCACACTTTCTATTTTAAAGTTCACACTTCATATTCTTTTCTTTTTTAATCAACAAAGTAAATTCTCATTCAACTCAAGCGACAGTGGATACATACCTTCCCCACTGCCAACAATATGGGCAAATAAGAAAAGTGGCATGCTCATTTCAAAAAAAAGAAAAGTGGCATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00670 ko01100 ko01110 ko01130 ko01523 map00230 map00670 map01100 map01110 map01130 map01523
Pfam Domains
Protein Families
DDE_Tnp_1_7 DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

419

Amino Acids

46.86

Weight (kDa)

9.74

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 110 - 156 1.3e-07 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000169)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13681 FvH4_3g25832 FvH4_3g26062 FvH4_4g08041 FvH4_4g13361 FvH4_4g16721 FvH4_4g21881 FvH4_6g07632 FvH4_6g17062 FvH4_6g26370 FvH4_7g07641
rosa_chinensis RchiOBHm_Chr1g0320861 RchiOBHm_Chr2g0109251 RchiOBHm_Chr3g0472781 RchiOBHm_Chr6g0288921 RchiOBHm_Chr7g0191711 RchiOBHm_Chr7g0238801
rosa_laevigata RLG00000007550 RLG00000036731
rosa_multiflora Rmu_co8018808.1_g000001 Rmu_co8401665.1_g000001 Rmu_co8461051.1_g000001 Rmu_sc0000144.1_g000042 Rmu_sc0000144.1_g000051 Rmu_sc0000160.1_g000018 Rmu_sc0000245.1_g000011 Rmu_sc0000299.1_g000021 Rmu_sc0000348.1_g000003 Rmu_sc0000367.1_g000120 Rmu_sc0000424.1_g000037 Rmu_sc0000427.1_g000006 Rmu_sc0000427.1_g000023 Rmu_sc0000427.1_g000037 Rmu_sc0000604.1_g000005 Rmu_sc0000605.1_g000060 Rmu_sc0000621.1_g000030 Rmu_sc0000693.1_g000017 Rmu_sc0000708.1_g000012 Rmu_sc0000918.1_g000031 Rmu_sc0000953.1_g000027 Rmu_sc0001030.1_g000008 Rmu_sc0001030.1_g000011 Rmu_sc0001051.1_g000026 Rmu_sc0001055.1_g000001 Rmu_sc0001081.1_g000001 Rmu_sc0001175.1_g000002 Rmu_sc0001176.1_g000026 Rmu_sc0001181.1_g000010 Rmu_sc0001225.1_g000003 Rmu_sc0001371.1_g000008 Rmu_sc0001433.1_g000012 Rmu_sc0001507.1_g000009 Rmu_sc0001643.1_g000014 Rmu_sc0001879.1_g000002 Rmu_sc0001890.1_g000006 Rmu_sc0002129.1_g000039 Rmu_sc0002356.1_g000008 Rmu_sc0002358.1_g000003 Rmu_sc0002553.1_g000024 Rmu_sc0002826.1_g000007 Rmu_sc0002872.1_g000007 Rmu_sc0003323.1_g000026 Rmu_sc0003410.1_g000001 Rmu_sc0003511.1_g000042 Rmu_sc0003541.1_g000036 Rmu_sc0003605.1_g000048 Rmu_sc0003689.1_g000014 Rmu_sc0003692.1_g000010 Rmu_sc0003720.1_g000001 Rmu_sc0003813.1_g000003 Rmu_sc0003872.1_g000005 Rmu_sc0004010.1_g000012 Rmu_sc0004165.1_g000092 Rmu_sc0004230.1_g000013 Rmu_sc0004318.1_g000020 Rmu_sc0004353.1_g000004 Rmu_sc0004617.1_g000005 Rmu_sc0004617.1_g000006 Rmu_sc0004658.1_g000005 Rmu_sc0005356.1_g000008 Rmu_sc0005994.1_g000014 Rmu_sc0006388.1_g000004 Rmu_sc0006674.1_g000020 Rmu_sc0006898.1_g000008 Rmu_sc0007286.1_g000005 Rmu_sc0007476.1_g000002 Rmu_sc0007671.1_g000005 Rmu_sc0007991.1_g000008 Rmu_sc0008131.1_g000008 Rmu_sc0008163.1_g000011 Rmu_sc0008322.1_g000004 Rmu_sc0008334.1_g000017 Rmu_sc0008835.1_g000009 Rmu_sc0008835.1_g000011 Rmu_sc0009954.1_g000006 Rmu_sc0010050.1_g000001 Rmu_sc0010317.1_g000011 Rmu_sc0010426.1_g000002 Rmu_sc0011111.1_g000005 Rmu_sc0014614.1_g000003 Rmu_sc0014688.1_g000002 Rmu_sc0015167.1_g000003 Rmu_sc0015936.1_g000003 Rmu_sc0017323.1_g000003 Rmu_sc0018541.1_g000001 Rmu_sc0019370.1_g000003 Rmu_sc0020444.1_g000001 Rmu_sc0020600.1_g000003 Rmu_sc0021238.1_g000001 Rmu_sc0022909.1_g000001 Rmu_sc0030397.1_g000001 Rmu_sc0030484.1_g000002 Rmu_sc0035139.1_g000001 Rmu_sc0035719.1_g000001 Rmu_ssc0000287.1_g000010 Rmu_ssc0000451.1_g000005
rosa_roxburghii Rroxscaffold_1G00046590 Rroxscaffold_1G00050100 Rroxscaffold_2G00079390 Rroxscaffold_2G00088790 Rroxscaffold_2G00097170 Rroxscaffold_2G00126350 Rroxscaffold_3G00221550 Rroxscaffold_3G00222700 Rroxscaffold_3G00236390 Rroxscaffold_3G00254000 Rroxscaffold_3G00260090 Rroxscaffold_3G00263600 Rroxscaffold_5G00336900
rosa_rugosa Rorug01G0048200 Rorug01G0172000 Rorug01G0294000 Rorug01G0308200 Rorug01G0355300 Rorug02G0000900 Rorug02G0129600 Rorug02G0136800 Rorug02G0179700 Rorug02G0251500 Rorug02G0305800 Rorug02G0305900 Rorug02G0373800 Rorug02G0396100 Rorug02G0411300 Rorug02G0411400 Rorug02G0411500 Rorug02G0460200 Rorug03G0187400 Rorug03G0299000 Rorug04G0041700 Rorug04G0130800 Rorug04G0168300 Rorug04G0198700 Rorug05G0095100 Rorug05G0194100 Rorug05G0205300 Rorug05G0235300 Rorug06G0088100.1 Rorug06G0120100 Rorug06G0164300 Rorug07G0163600 Rorug07G0163700 Rorug07G0236200 Rorug07G0307200 RorugPtG0006100
rosa_samantha Rh1DG036900 Rh3BG176200 Rh4CG455100 Rh6AG142400 Rh6AG263000 Rh6CG138900 Rh6DG126200 Rh7CG393300 Rh7CG447500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 411, 796
Acc36I ACCTGC 2 cut(s) 411, 796
AccII CGCG 3 cut(s) 12, 578, 849
AccIII TCCGGA 1 cut(s) 309
AciI CCGC 5 cut(s) 76, 264, 576, 578, 898
AclI AACGTT 1 cut(s) 911
AclWI GGATC 4 cut(s) 15, 160, 320, 464
AcoI YGGCCR 2 cut(s) 895, 1028
AcsI RAATTY 4 cut(s) 207, 235, 903, 1148
AcuI CTGAAG 1 cut(s) 992
AdeI CACNNNGTG 1 cut(s) 434
AfiI CCNNNNNNNGG 4 cut(s) 97, 190, 652, 805
AgeI ACCGGT 1 cut(s) 1017
AgsI TTSAA 4 cut(s) 407, 1044, 1159, 1236
AjnI CCWGG 4 cut(s) 159, 694, 813, 1061
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AloI GAACNNNNNNTCC 2 cut(s) 155, 187
AluBI AGCT 3 cut(s) 630, 779, 1036
AluI AGCT 3 cut(s) 630, 779, 1036
Alw21I GWGCWC 1 cut(s) 428
AlwI GGATC 4 cut(s) 15, 160, 320, 464
Aor13HI TCCGGA 1 cut(s) 309
AoxI GGCC 3 cut(s) 579, 895, 1028
ApeKI GCWGC 1 cut(s) 1012
ApoI RAATTY 4 cut(s) 207, 235, 903, 1148
AsiGI ACCGGT 1 cut(s) 1017
AspA2I CCTAGG 1 cut(s) 494
AspLEI GCGC 3 cut(s) 715, 849, 851
AspS9I GGNCC 2 cut(s) 811, 1059
AsuC2I CCSGG 2 cut(s) 36, 513
AsuHPI GGTGA 1 cut(s) 565
AvaII GGWCC 2 cut(s) 811, 1059
AvrII CCTAGG 1 cut(s) 494
BalI TGGCCA 1 cut(s) 1030
BanII GRGCYC 3 cut(s) 121, 441, 518
BbsI GAAGAC 1 cut(s) 625
Bbv12I GWGCWC 1 cut(s) 428
BbvI GCAGC 1 cut(s) 1024
BccI CCATC 4 cut(s) 137, 680, 703, 737
BciT130I CCWGG 4 cut(s) 161, 696, 815, 1063
BciVI GTATCC 1 cut(s) 1169
BcnI CCSGG 2 cut(s) 36, 513
BfaI CTAG 4 cut(s) 350, 495, 668, 1258
BfuAI ACCTGC 2 cut(s) 411, 796
BfuI GTATCC 1 cut(s) 1169
BisI GCNGC 4 cut(s) 265, 579, 898, 1013
BlnI CCTAGG 1 cut(s) 494
BlsI GCNGC 4 cut(s) 266, 580, 899, 1014
Bme1390I CCNGG 6 cut(s) 36, 161, 513, 696, 815, 1063
Bme18I GGWCC 2 cut(s) 811, 1059
BmgT120I GGNCC 2 cut(s) 811, 1059
BmiI GGNNCC 3 cut(s) 384, 535, 1060
BmrFI CCNGG 6 cut(s) 36, 161, 513, 696, 815, 1063
BmrI ACTGGG 1 cut(s) 116
BmsI GCATC 2 cut(s) 57, 459
BmuI ACTGGG 1 cut(s) 116
BpiI GAAGAC 1 cut(s) 625
BpmI CTGGAG 1 cut(s) 143
BpuEI CTTGAG 1 cut(s) 1147
BpuMI CCSGG 2 cut(s) 36, 513
BsaBI GATNNNNATC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 91, 494, 576, 993
BsaWI WCCGGW 2 cut(s) 309, 1017
BsaXI ACNNNNNCTCC 2 cut(s) 155, 185
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 190, 652, 805
Bse118I RCCGGY 2 cut(s) 652, 1017
Bse1I ACTGG 6 cut(s) 122, 169, 341, 726, 763, 966
Bse8I GATNNNNATC 1 cut(s) 317
BseAI TCCGGA 1 cut(s) 309
BseBI CCWGG 4 cut(s) 161, 696, 815, 1063
BseDI CCNNGG 4 cut(s) 91, 494, 576, 993
BseGI GGATG 1 cut(s) 306
BseJI GATNNNNATC 1 cut(s) 317
BseLI CCNNNNNNNGG 4 cut(s) 97, 190, 652, 805
BseMII CTCAG 1 cut(s) 693
BseNI ACTGG 6 cut(s) 122, 169, 341, 726, 763, 966
BsePI GCGCGC 1 cut(s) 847
BseRI GAGGAG 1 cut(s) 1090
BseX3I CGGCCG 1 cut(s) 895
BseXI GCAGC 1 cut(s) 1024
BseYI CCCAGC 1 cut(s) 452
BsgI GTGCAG 1 cut(s) 824
Bsh1236I CGCG 3 cut(s) 12, 578, 849
Bsh1285I CGRYCG 1 cut(s) 898
BshFI GGCC 3 cut(s) 581, 897, 1030
BshTI ACCGGT 1 cut(s) 1017
BsiEI CGRYCG 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 428
BsiSI CCGG 5 cut(s) 36, 310, 512, 653, 1018
BslFI GGGAC 4 cut(s) 578, 786, 975, 1072
BslI CCNNNNNNNGG 4 cut(s) 97, 190, 652, 805
BsmFI GGGAC 4 cut(s) 578, 786, 975, 1072
BsnI GGCC 3 cut(s) 581, 897, 1030
Bsp1286I GDGCHC 4 cut(s) 121, 428, 441, 518
Bsp13I TCCGGA 1 cut(s) 309
Bsp143I GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
Bsp68I TCGCGA 1 cut(s) 12
BspACI CCGC 5 cut(s) 76, 264, 576, 578, 898
BspANI GGCC 3 cut(s) 581, 897, 1030
BspCNI CTCAG 1 cut(s) 692
BspEI TCCGGA 1 cut(s) 309
BspFNI CGCG 3 cut(s) 12, 578, 849
BspLI GGNNCC 3 cut(s) 384, 535, 1060
BspMI ACCTGC 2 cut(s) 411, 796
BspPI GGATC 4 cut(s) 15, 160, 320, 464
BspQI GCTCTTC 1 cut(s) 446
BsrFI RCCGGY 2 cut(s) 652, 1017
BsrI ACTGG 6 cut(s) 122, 169, 341, 726, 763, 966
BssAI RCCGGY 2 cut(s) 652, 1017
BssECI CCNNGG 4 cut(s) 91, 494, 576, 993
BssHII GCGCGC 1 cut(s) 847
BssMI GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
BssT1I CCWWGG 2 cut(s) 494, 993
Bst2UI CCWGG 4 cut(s) 161, 696, 815, 1063
Bst4CI ACNGT 4 cut(s) 432, 800, 950, 1172
Bst6I CTCTTC 2 cut(s) 446, 1042
BstC8I GCNNGC 7 cut(s) 152, 654, 849, 918, 1038, 1226, 1255
BstDEI CTNAG 3 cut(s) 259, 679, 834
BstDSI CCRYGG 1 cut(s) 576
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 3 cut(s) 12, 578, 849
BstHHI GCGC 3 cut(s) 715, 849, 851
BstKTI GATC 7 cut(s) 10, 115, 168, 315, 321, 459, 615
BstMBI GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
BstMCI CGRYCG 1 cut(s) 898
BstMWI GCNNNNNNNGC 1 cut(s) 346
BstNI CCWGG 4 cut(s) 161, 696, 815, 1063
BstNSI RCATGY 2 cut(s) 1228, 1257
BstSCI CCNGG 6 cut(s) 34, 159, 511, 694, 813, 1061
BstUI CGCG 3 cut(s) 12, 578, 849
BstV1I GCAGC 1 cut(s) 1024
BstV2I GAAGAC 1 cut(s) 625
BstX2I RGATCY 3 cut(s) 165, 312, 456
BstXI CCANNNNNNTGG 1 cut(s) 1203
BstYI RGATCY 3 cut(s) 165, 312, 456
BstZI CGGCCG 1 cut(s) 895
BsuI GTATCC 1 cut(s) 1169
BsuRI GGCC 3 cut(s) 581, 897, 1030
BtgI CCRYGG 1 cut(s) 576
BtgZI GCGATG 1 cut(s) 722
BtsCI GGATG 1 cut(s) 306
BtsI GCAGTG 2 cut(s) 662, 1190
BtsIMutI CAGTG 6 cut(s) 437, 662, 955, 959, 1177, 1190
BtuMI TCGCGA 1 cut(s) 12
BveI ACCTGC 2 cut(s) 411, 796
Cac8I GCNNGC 7 cut(s) 152, 654, 849, 918, 1038, 1226, 1255
CfoI GCGC 3 cut(s) 715, 849, 851
Cfr10I RCCGGY 2 cut(s) 652, 1017
Cfr13I GGNCC 2 cut(s) 811, 1059
Cfr42I CCGCGG 1 cut(s) 579
CspAI ACCGGT 1 cut(s) 1017
CviAII CATG 5 cut(s) 71, 326, 716, 1225, 1254
DdeI CTNAG 3 cut(s) 259, 679, 834
DpnI GATC 7 cut(s) 9, 114, 167, 314, 320, 458, 614
DpnII GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
DraI TTTAAA 1 cut(s) 1104
DraIII CACNNNGTG 1 cut(s) 434
EaeI YGGCCR 2 cut(s) 895, 1028
EagI CGGCCG 1 cut(s) 895
Eam1104I CTCTTC 2 cut(s) 446, 1042
EarI CTCTTC 2 cut(s) 446, 1042
EclXI CGGCCG 1 cut(s) 895
Eco130I CCWWGG 2 cut(s) 494, 993
Eco24I GRGCYC 3 cut(s) 121, 441, 518
Eco32I GATATC 1 cut(s) 823
Eco47I GGWCC 2 cut(s) 811, 1059
Eco52I CGGCCG 1 cut(s) 895
Eco57I CTGAAG 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 207
EcoRII CCWGG 4 cut(s) 159, 694, 813, 1061
EcoRV GATATC 1 cut(s) 823
EcoT14I CCWWGG 2 cut(s) 494, 993
EcoT22I ATGCAT 1 cut(s) 72
EcoT38I GRGCYC 3 cut(s) 121, 441, 518
ErhI CCWWGG 2 cut(s) 494, 993
FaeI CATG 5 cut(s) 74, 329, 719, 1228, 1257
FalI AAGNNNNNCTT 4 cut(s) 551, 583, 1077, 1109
FaqI GGGAC 4 cut(s) 578, 786, 975, 1072
FatI CATG 5 cut(s) 70, 325, 715, 1224, 1253
Fnu4HI GCNGC 4 cut(s) 265, 579, 898, 1013
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 3 cut(s) 121, 441, 518
Fsp4HI GCNGC 4 cut(s) 265, 579, 898, 1013
FspBI CTAG 4 cut(s) 350, 495, 668, 1258
GlaI GCGC 3 cut(s) 714, 848, 850
GluI GCNGC 4 cut(s) 265, 579, 898, 1013
GsaI CCCAGC 1 cut(s) 456
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 3 cut(s) 581, 897, 1030
HapII CCGG 5 cut(s) 36, 310, 512, 653, 1018
HhaI GCGC 3 cut(s) 715, 849, 851
Hin1II CATG 5 cut(s) 74, 329, 719, 1228, 1257
Hin6I GCGC 3 cut(s) 713, 847, 849
HinP1I GCGC 3 cut(s) 713, 847, 849
HinfI GANTC 6 cut(s) 62, 303, 621, 659, 677, 1066
HpaII CCGG 5 cut(s) 36, 310, 512, 653, 1018
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 1 cut(s) 1110
Hpy188I TCNGA 4 cut(s) 92, 617, 676, 972
Hpy188III TCNNGA 7 cut(s) 11, 26, 204, 310, 316, 592, 1044
Hpy8I GTNNAC 1 cut(s) 1110
HpyAV CCTTC 2 cut(s) 280, 1194
HpyCH4III ACNGT 4 cut(s) 432, 800, 950, 1172
HpyCH4IV ACGT 1 cut(s) 911
HpyCH4V TGCA 4 cut(s) 70, 805, 861, 916
HpyF10VI GCNNNNNNNGC 1 cut(s) 346
HpyF3I CTNAG 3 cut(s) 259, 679, 834
HpySE526I ACGT 1 cut(s) 911
Hsp92II CATG 5 cut(s) 74, 329, 719, 1228, 1257
HspAI GCGC 3 cut(s) 713, 847, 849
Kpn2I TCCGGA 1 cut(s) 309
KroI GCCGGC 1 cut(s) 652
KroNI GCCGGC 1 cut(s) 654
KspI CCGCGG 1 cut(s) 579
Kzo9I GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
LguI GCTCTTC 1 cut(s) 446
LmnI GCTCC 1 cut(s) 844
Lsp1109I GCAGC 1 cut(s) 1024
LweI GCATC 2 cut(s) 57, 459
MaeI CTAG 4 cut(s) 350, 495, 668, 1258
MaeII ACGT 1 cut(s) 911
MaeIII GTNAC 2 cut(s) 964, 1087
MalI GATC 7 cut(s) 9, 114, 167, 314, 320, 458, 614
MboI GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
MboII GAAGA 9 cut(s) 236, 433, 516, 562, 607, 630, 985, 988, 1059
MflI RGATCY 3 cut(s) 165, 312, 456
MhlI GDGCHC 4 cut(s) 121, 428, 441, 518
MlsI TGGCCA 1 cut(s) 1030
MluCI AATT 4 cut(s) 207, 235, 903, 1148
MluNI TGGCCA 1 cut(s) 1030
MlyI GAGTC 5 cut(s) 56, 312, 615, 671, 1060
MmeI TCCRAC 2 cut(s) 199, 932
MnlI CCTC 9 cut(s) 86, 165, 555, 592, 811, 877, 930, 1043, 1068
Mox20I TGGCCA 1 cut(s) 1030
Mph1103I ATGCAT 1 cut(s) 72
MroI TCCGGA 1 cut(s) 309
MroNI GCCGGC 1 cut(s) 652
MscI TGGCCA 1 cut(s) 1030
MseI TTAA 5 cut(s) 294, 636, 882, 1103, 1134
MslI CAYNNNNRTG 1 cut(s) 759
Msp20I TGGCCA 1 cut(s) 1030
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 5 cut(s) 36, 310, 512, 653, 1018
MspR9I CCNGG 6 cut(s) 36, 161, 513, 696, 815, 1063
MvaI CCWGG 4 cut(s) 161, 696, 815, 1063
MvnI CGCG 3 cut(s) 12, 578, 849
MwoI GCNNNNNNNGC 1 cut(s) 346
NaeI GCCGGC 1 cut(s) 654
NciI CCSGG 2 cut(s) 36, 513
NdeII GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
NgoMIV GCCGGC 1 cut(s) 652
NlaIII CATG 5 cut(s) 74, 329, 719, 1228, 1257
NlaIV GGNNCC 3 cut(s) 384, 535, 1060
NmeAIII GCCGAG 1 cut(s) 873
NmuCI GTSAC 1 cut(s) 1087
NruI TCGCGA 1 cut(s) 12
NsiI ATGCAT 1 cut(s) 72
NspI RCATGY 2 cut(s) 1228, 1257
PaeI GCATGC 2 cut(s) 1228, 1257
PaqCI CACCTGC 2 cut(s) 411, 796
PauI GCGCGC 1 cut(s) 847
PciSI GCTCTTC 1 cut(s) 446
PdiI GCCGGC 1 cut(s) 654
PfeI GAWTC 1 cut(s) 659
PfoI TCCNGGA 2 cut(s) 159, 813
PinAI ACCGGT 1 cut(s) 1017
PkrI GCNGC 4 cut(s) 266, 580, 899, 1014
PleI GAGTC 5 cut(s) 56, 311, 615, 671, 1060
PpsI GAGTC 5 cut(s) 56, 311, 615, 671, 1060
Psp1406I AACGTT 1 cut(s) 911
Psp6I CCWGG 4 cut(s) 159, 694, 813, 1061
PspFI CCCAGC 1 cut(s) 452
PspGI CCWGG 4 cut(s) 159, 694, 813, 1061
PspN4I GGNNCC 3 cut(s) 384, 535, 1060
PspPI GGNCC 2 cut(s) 811, 1059
PsrI GAACNNNNNNTAC 2 cut(s) 375, 407
PsuI RGATCY 3 cut(s) 165, 312, 456
PteI GCGCGC 1 cut(s) 847
RruI TCGCGA 1 cut(s) 12
RseI CAYNNNNRTG 1 cut(s) 759
SacII CCGCGG 1 cut(s) 579
SapI GCTCTTC 1 cut(s) 446
SaqAI TTAA 5 cut(s) 294, 636, 882, 1103, 1134
SatI GCNGC 4 cut(s) 265, 579, 898, 1013
Sau3AI GATC 7 cut(s) 7, 112, 165, 312, 318, 456, 612
Sau96I GGNCC 2 cut(s) 811, 1059
SchI GAGTC 5 cut(s) 56, 312, 615, 671, 1060
ScrFI CCNGG 6 cut(s) 36, 161, 513, 696, 815, 1063
SduI GDGCHC 4 cut(s) 121, 428, 441, 518
SetI ASST 9 cut(s) 97, 425, 632, 781, 810, 914, 1038, 1054, 1186
SfaNI GCATC 2 cut(s) 57, 459
Sfr303I CCGCGG 1 cut(s) 579
SgrBI CCGCGG 1 cut(s) 579
SinI GGWCC 2 cut(s) 811, 1059
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 1 cut(s) 1162
SmoI CTYRAG 1 cut(s) 1162
SphI GCATGC 2 cut(s) 1228, 1257
Sse9I AATT 4 cut(s) 207, 235, 903, 1148
SsiI CCGC 5 cut(s) 76, 264, 576, 578, 898
SspMI CTAG 4 cut(s) 350, 495, 668, 1258
StyD4I CCNGG 6 cut(s) 34, 159, 511, 694, 813, 1061
StyI CCWWGG 2 cut(s) 494, 993
TaaI ACNGT 4 cut(s) 432, 800, 950, 1172
TaiI ACGT 1 cut(s) 914
TaqI TCGA 3 cut(s) 65, 115, 624
TasI AATT 4 cut(s) 207, 235, 903, 1148
TauI GCSGC 3 cut(s) 267, 581, 900
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 5 cut(s) 294, 636, 882, 1103, 1134
Tru9I TTAA 5 cut(s) 294, 636, 882, 1103, 1134
TscAI CASTG 6 cut(s) 437, 669, 955, 966, 1177, 1197
TseFI GTSAC 1 cut(s) 1087
TseI GCWGC 1 cut(s) 1012
Tsp45I GTSAC 1 cut(s) 1087
TspDTI ATGAA 3 cut(s) 237, 732, 1106
TspGWI ACGGA 1 cut(s) 173
TspRI CASTG 6 cut(s) 437, 669, 955, 966, 1177, 1197
VpaK11BI GGWCC 2 cut(s) 811, 1059
XapI RAATTY 4 cut(s) 207, 235, 903, 1148
XceI RCATGY 2 cut(s) 1228, 1257
XmaJI CCTAGG 1 cut(s) 494
XspI CTAG 4 cut(s) 350, 495, 668, 1258
Zsp2I ATGCAT 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.