Rmu_sc0001181.1_g000010

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001181.1
OG0000169
Physical Location & Seq
Forward (+)
47355 .. 48455
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001181.1_g000010.1.cds

Sequence Viewer

Length: 1101 bp
atggcttctattgatgctgtgaccacccaatcttttctaattggcaaacctcggacccataagctggttgatccaaaggatctcaaagcccacttctatcgtacttggatggtggataaagatttcaggattcaggaacgggctgggaatttttcttgttctcctttgaatcagtacatgatcgtaacaaggtactctaagggggggggggtctggtgctttgatcaagccccggtttactttgaagattatgatggcgttaagctactgtatgaagttcagttcaagcatgttcgagtttggggtagggtttctggaatctcacccttgtatgaagaacctgagaacttcactctgatcggtaatctcttgggtggtttcttggattatgataagaaggaattcaagaagggatcaattaaggttttatttactcatgatatctctaaaccggtcttacttgaacgcaaagtttttctggatgttggtgttgaacccgttctgcagtttcaatttgagcatctaaaagggagatgctcacaatgtggtctcatcacgcatgttgaaatttcttgtgaggagcccaacacgattgtctctacacttagggttttgaactttcccgaaccttcggcctctgaaggctccttctccttctctgctaagaataagtgtgatctcttcactgcttcctcctccatcccaaagaagaagaagcctgttatcctcctagtggagcgccccattcccaatcctgttttccctactggttcgtcacctatagcagtggctgttgattctgcgatggagcatgggctggaatgtgatcgggttccagatttggagcctaccattgacactgtttgtgatgaatctacaagtatgatgcaggtggtgcagcaggagggtttgcattccaagaaacgggaccgcaatggtggttgcaatccttctcccaagaagttcaaaaccatcttgggtggcaaggttccaactttacagactgatgctttggggttgattgaagatgacaatgatgttactcttgtaacttcgaagaagaggactggcatgcctcttggaagcaagaataagatctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

366

Amino Acids

40.87

Weight (kDa)

7.99

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000169)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g13681 FvH4_3g25832 FvH4_3g26062 FvH4_4g08041 FvH4_4g13361 FvH4_4g16721 FvH4_4g21881 FvH4_6g07632 FvH4_6g17062 FvH4_6g26370 FvH4_7g07641
rosa_chinensis RchiOBHm_Chr1g0320861 RchiOBHm_Chr2g0109251 RchiOBHm_Chr3g0472781 RchiOBHm_Chr6g0288921 RchiOBHm_Chr7g0191711 RchiOBHm_Chr7g0238801
rosa_laevigata RLG00000007550 RLG00000036731
rosa_multiflora Rmu_co8018808.1_g000001 Rmu_co8401665.1_g000001 Rmu_co8461051.1_g000001 Rmu_sc0000144.1_g000042 Rmu_sc0000144.1_g000051 Rmu_sc0000160.1_g000018 Rmu_sc0000245.1_g000011 Rmu_sc0000299.1_g000021 Rmu_sc0000348.1_g000003 Rmu_sc0000367.1_g000120 Rmu_sc0000424.1_g000037 Rmu_sc0000427.1_g000006 Rmu_sc0000427.1_g000023 Rmu_sc0000427.1_g000037 Rmu_sc0000604.1_g000005 Rmu_sc0000605.1_g000060 Rmu_sc0000621.1_g000030 Rmu_sc0000693.1_g000017 Rmu_sc0000708.1_g000012 Rmu_sc0000918.1_g000031 Rmu_sc0000953.1_g000027 Rmu_sc0001030.1_g000008 Rmu_sc0001030.1_g000011 Rmu_sc0001051.1_g000026 Rmu_sc0001055.1_g000001 Rmu_sc0001081.1_g000001 Rmu_sc0001175.1_g000002 Rmu_sc0001176.1_g000026 Rmu_sc0001181.1_g000010 Rmu_sc0001225.1_g000003 Rmu_sc0001371.1_g000008 Rmu_sc0001433.1_g000012 Rmu_sc0001507.1_g000009 Rmu_sc0001643.1_g000014 Rmu_sc0001879.1_g000002 Rmu_sc0001890.1_g000006 Rmu_sc0002129.1_g000039 Rmu_sc0002356.1_g000008 Rmu_sc0002358.1_g000003 Rmu_sc0002553.1_g000024 Rmu_sc0002826.1_g000007 Rmu_sc0002872.1_g000007 Rmu_sc0003323.1_g000026 Rmu_sc0003410.1_g000001 Rmu_sc0003511.1_g000042 Rmu_sc0003541.1_g000036 Rmu_sc0003605.1_g000048 Rmu_sc0003689.1_g000014 Rmu_sc0003692.1_g000010 Rmu_sc0003720.1_g000001 Rmu_sc0003813.1_g000003 Rmu_sc0003872.1_g000005 Rmu_sc0004010.1_g000012 Rmu_sc0004165.1_g000092 Rmu_sc0004230.1_g000013 Rmu_sc0004318.1_g000020 Rmu_sc0004353.1_g000004 Rmu_sc0004617.1_g000005 Rmu_sc0004617.1_g000006 Rmu_sc0004658.1_g000005 Rmu_sc0005356.1_g000008 Rmu_sc0005994.1_g000014 Rmu_sc0006388.1_g000004 Rmu_sc0006674.1_g000020 Rmu_sc0006898.1_g000008 Rmu_sc0007286.1_g000005 Rmu_sc0007476.1_g000002 Rmu_sc0007671.1_g000005 Rmu_sc0007991.1_g000008 Rmu_sc0008131.1_g000008 Rmu_sc0008163.1_g000011 Rmu_sc0008322.1_g000004 Rmu_sc0008334.1_g000017 Rmu_sc0008835.1_g000009 Rmu_sc0008835.1_g000011 Rmu_sc0009954.1_g000006 Rmu_sc0010050.1_g000001 Rmu_sc0010317.1_g000011 Rmu_sc0010426.1_g000002 Rmu_sc0011111.1_g000005 Rmu_sc0014614.1_g000003 Rmu_sc0014688.1_g000002 Rmu_sc0015167.1_g000003 Rmu_sc0015936.1_g000003 Rmu_sc0017323.1_g000003 Rmu_sc0018541.1_g000001 Rmu_sc0019370.1_g000003 Rmu_sc0020444.1_g000001 Rmu_sc0020600.1_g000003 Rmu_sc0021238.1_g000001 Rmu_sc0022909.1_g000001 Rmu_sc0030397.1_g000001 Rmu_sc0030484.1_g000002 Rmu_sc0035139.1_g000001 Rmu_sc0035719.1_g000001 Rmu_ssc0000287.1_g000010 Rmu_ssc0000451.1_g000005
rosa_roxburghii Rroxscaffold_1G00046590 Rroxscaffold_1G00050100 Rroxscaffold_2G00079390 Rroxscaffold_2G00088790 Rroxscaffold_2G00097170 Rroxscaffold_2G00126350 Rroxscaffold_3G00221550 Rroxscaffold_3G00222700 Rroxscaffold_3G00236390 Rroxscaffold_3G00254000 Rroxscaffold_3G00260090 Rroxscaffold_3G00263600 Rroxscaffold_5G00336900
rosa_rugosa Rorug01G0048200 Rorug01G0172000 Rorug01G0294000 Rorug01G0308200 Rorug01G0355300 Rorug02G0000900 Rorug02G0129600 Rorug02G0136800 Rorug02G0179700 Rorug02G0251500 Rorug02G0305800 Rorug02G0305900 Rorug02G0373800 Rorug02G0396100 Rorug02G0411300 Rorug02G0411400 Rorug02G0411500 Rorug02G0460200 Rorug03G0187400 Rorug03G0299000 Rorug04G0041700 Rorug04G0130800 Rorug04G0168300 Rorug04G0198700 Rorug05G0095100 Rorug05G0194100 Rorug05G0205300 Rorug05G0235300 Rorug06G0088100.1 Rorug06G0120100 Rorug06G0164300 Rorug07G0163600 Rorug07G0163700 Rorug07G0236200 Rorug07G0307200 RorugPtG0006100
rosa_samantha Rh1DG036900 Rh3BG176200 Rh4CG455100 Rh6AG142400 Rh6AG263000 Rh6CG138900 Rh6DG126200 Rh7CG393300 Rh7CG447500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 880
Acc36I ACCTGC 1 cut(s) 880
AccB7I CCANNNNNTGG 1 cut(s) 64
AciI CCGC 1 cut(s) 931
AclWI GGATC 3 cut(s) 65, 87, 421
AcsI RAATTY 3 cut(s) 148, 401, 567
AcuI CTGAAG 1 cut(s) 660
AdeI CACNNNGTG 1 cut(s) 545
AfaI GTAC 3 cut(s) 103, 176, 194
AfiI CCNNNNNNNGG 3 cut(s) 64, 733, 924
AgeI ACCGGT 1 cut(s) 451
AjuI GAANNNNNNNTTGG 2 cut(s) 743, 775
AluBI AGCT 2 cut(s) 64, 265
AluI AGCT 2 cut(s) 64, 265
Alw26I GTCTC 2 cut(s) 554, 601
AlwI GGATC 3 cut(s) 65, 87, 421
AlwNI CAGNNNCTG 1 cut(s) 791
AoxI GGCC 1 cut(s) 633
ApeKI GCWGC 1 cut(s) 898
ApoI RAATTY 3 cut(s) 148, 401, 567
ArsI GACNNNNNNTTYG 2 cut(s) 994, 1026
AsiGI ACCGGT 1 cut(s) 451
Asp700I GAANNNNTTC 2 cut(s) 401, 498
AspLEI GCGC 1 cut(s) 741
AspS9I GGNCC 2 cut(s) 54, 928
AsuC2I CCSGG 1 cut(s) 233
AsuHPI GGTGA 2 cut(s) 315, 768
AsuII TTCGAA 1 cut(s) 1055
AvaII GGWCC 2 cut(s) 54, 928
BanII GRGCYC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 910
BccI CCATC 5 cut(s) 103, 248, 707, 799, 980
BclI TGATCA 1 cut(s) 223
BcnI CCSGG 1 cut(s) 233
BcoDI GTCTC 2 cut(s) 554, 601
BfaI CTAG 2 cut(s) 731, 1099
BfmI CTRYAG 2 cut(s) 503, 780
BfoI RGCGCY 1 cut(s) 742
BfuAI ACCTGC 1 cut(s) 880
BglII AGATCT 1 cut(s) 1095
BisI GCNGC 1 cut(s) 899
BlsI GCNGC 1 cut(s) 900
Bme1390I CCNGG 1 cut(s) 233
Bme18I GGWCC 2 cut(s) 54, 928
BmgT120I GGNCC 2 cut(s) 54, 928
BmiI GGNNCC 7 cut(s) 56, 582, 646, 834, 846, 929, 990
BmrFI CCNGG 1 cut(s) 233
BmsI GCATC 5 cut(s) 4, 524, 529, 876, 997
Bpu14I TTCGAA 1 cut(s) 1055
BpuMI CCSGG 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 554
BsaJI CCNNGG 2 cut(s) 50, 231
BsaWI WCCGGW 1 cut(s) 451
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 733, 924
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 772, 1072
Bse3DI GCAATG 1 cut(s) 940
BseDI CCNNGG 2 cut(s) 50, 231
BseGI GGATG 3 cut(s) 114, 487, 699
BseLI CCNNNNNNNGG 3 cut(s) 64, 733, 924
BseMI GCAATG 1 cut(s) 940
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 2 cut(s) 772, 1072
BseRI GAGGAG 2 cut(s) 593, 685
BseXI GCAGC 1 cut(s) 910
BseYI CCCAGC 1 cut(s) 143
BsgI GTGCAG 1 cut(s) 917
BshFI GGCC 1 cut(s) 635
BshTI ACCGGT 1 cut(s) 451
BsiSI CCGG 2 cut(s) 233, 452
BslFI GGGAC 1 cut(s) 941
BslI CCNNNNNNNGG 3 cut(s) 64, 733, 924
BsmAI GTCTC 2 cut(s) 554, 601
BsmFI GGGAC 1 cut(s) 941
BsmI GAATGC 1 cut(s) 913
BsnI GGCC 1 cut(s) 635
Bso31I GGTCTC 1 cut(s) 554
Bsp119I TTCGAA 1 cut(s) 1055
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
BspACI CCGC 1 cut(s) 931
BspANI GGCC 1 cut(s) 635
BspCNI CTCAG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 7 cut(s) 56, 582, 646, 834, 846, 929, 990
BspMAI CTGCAG 1 cut(s) 507
BspMI ACCTGC 1 cut(s) 880
BspPI GGATC 3 cut(s) 65, 87, 421
BspT104I TTCGAA 1 cut(s) 1055
BspTNI GGTCTC 1 cut(s) 554
BsrDI GCAATG 1 cut(s) 940
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 2 cut(s) 772, 1072
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 2 cut(s) 50, 231
BssMI GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
Bst4CI ACNGT 2 cut(s) 270, 862
Bst6I CTCTTC 2 cut(s) 686, 1055
BstAPI GCANNNNNTGC 1 cut(s) 895
BstBI TTCGAA 1 cut(s) 1055
BstC8I GCNNGC 1 cut(s) 1073
BstDEI CTNAG 4 cut(s) 198, 342, 605, 663
BstF5I GGATG 3 cut(s) 114, 487, 699
BstH2I RGCGCY 1 cut(s) 742
BstHHI GCGC 1 cut(s) 741
BstKTI GATC 9 cut(s) 73, 82, 183, 226, 360, 416, 679, 829, 1098
BstMAI GTCTC 2 cut(s) 554, 601
BstMBI GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
BstMWI GCNNNNNNNGC 1 cut(s) 895
BstNSI RCATGY 3 cut(s) 293, 563, 1075
BstSCI CCNGG 1 cut(s) 231
BstSFI CTRYAG 2 cut(s) 503, 780
BstV1I GCAGC 1 cut(s) 910
BstX2I RGATCY 2 cut(s) 79, 1095
BstYI RGATCY 2 cut(s) 79, 1095
BsuRI GGCC 1 cut(s) 635
BtgZI GCGATG 1 cut(s) 818
BtsCI GGATG 3 cut(s) 114, 487, 699
BtsI GCAGTG 2 cut(s) 684, 792
BtsIMutI CAGTG 3 cut(s) 684, 792, 858
BveI ACCTGC 1 cut(s) 880
Cac8I GCNNGC 1 cut(s) 1073
CaiI CAGNNNCTG 1 cut(s) 791
CciI TCATGA 1 cut(s) 436
CfoI GCGC 1 cut(s) 741
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 2 cut(s) 54, 928
Csp6I GTAC 3 cut(s) 102, 175, 193
CspAI ACCGGT 1 cut(s) 451
CviAII CATG 6 cut(s) 178, 290, 437, 560, 812, 1072
CviQI GTAC 3 cut(s) 102, 175, 193
DdeI CTNAG 4 cut(s) 198, 342, 605, 663
DpnI GATC 9 cut(s) 72, 81, 182, 225, 359, 415, 678, 828, 1097
DpnII GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
DraIII CACNNNGTG 1 cut(s) 545
Eam1104I CTCTTC 2 cut(s) 686, 1055
EarI CTCTTC 2 cut(s) 686, 1055
Eco24I GRGCYC 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 554
Eco32I GATATC 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 54, 928
Eco57I CTGAAG 1 cut(s) 660
EcoRI GAATTC 1 cut(s) 401
EcoRV GATATC 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 585
FaeI CATG 6 cut(s) 181, 293, 440, 563, 815, 1075
FaqI GGGAC 1 cut(s) 941
FatI CATG 6 cut(s) 177, 289, 436, 559, 811, 1071
FbaI TGATCA 1 cut(s) 223
Fnu4HI GCNGC 1 cut(s) 899
FokI GGATG 3 cut(s) 121, 494, 686
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 899
FspBI CTAG 2 cut(s) 731, 1099
GlaI GCGC 1 cut(s) 740
GluI GCNGC 1 cut(s) 899
GsaI CCCAGC 1 cut(s) 147
HaeII RGCGCY 1 cut(s) 742
HaeIII GGCC 1 cut(s) 635
HapII CCGG 2 cut(s) 233, 452
HhaI GCGC 1 cut(s) 741
Hin1II CATG 6 cut(s) 181, 293, 440, 563, 815, 1075
Hin6I GCGC 1 cut(s) 739
HinP1I GCGC 1 cut(s) 739
HinfI GANTC 5 cut(s) 130, 169, 318, 797, 872
HpaII CCGG 2 cut(s) 233, 452
HphI GGTGA 2 cut(s) 315, 768
Hpy166II GTNNAC 1 cut(s) 238
Hpy188I TCNGA 3 cut(s) 54, 357, 640
Hpy188III TCNNGA 8 cut(s) 127, 134, 315, 406, 437, 479, 623, 836
Hpy8I GTNNAC 1 cut(s) 238
HpyAV CCTTC 7 cut(s) 391, 403, 635, 639, 658, 664, 960
HpyCH4III ACNGT 2 cut(s) 270, 862
HpyCH4V TGCA 5 cut(s) 505, 889, 898, 913, 945
HpyF10VI GCNNNNNNNGC 1 cut(s) 895
HpyF3I CTNAG 4 cut(s) 198, 342, 605, 663
Hsp92II CATG 6 cut(s) 181, 293, 440, 563, 815, 1075
HspAI GCGC 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
LmnI GCTCC 5 cut(s) 580, 650, 736, 808, 844
Lsp1109I GCAGC 1 cut(s) 910
LweI GCATC 5 cut(s) 4, 524, 529, 876, 997
MaeI CTAG 2 cut(s) 731, 1099
MaeIII GTNAC 5 cut(s) 19, 184, 774, 1039, 1048
MalI GATC 9 cut(s) 72, 81, 182, 225, 359, 415, 678, 828, 1097
MboI GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
MboII GAAGA 8 cut(s) 257, 347, 673, 721, 724, 1037, 1069, 1072
MflI RGATCY 2 cut(s) 79, 1095
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 6 cut(s) 39, 148, 401, 417, 512, 567
MmeI TCCRAC 1 cut(s) 1016
MnlI CCTC 9 cut(s) 60, 571, 646, 703, 706, 737, 898, 1056, 1086
MroXI GAANNNNTTC 2 cut(s) 401, 498
MseI TTAA 2 cut(s) 261, 420
MspI CCGG 2 cut(s) 233, 452
MspR9I CCNGG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 913
MwoI GCNNNNNNNGC 1 cut(s) 895
NciI CCSGG 1 cut(s) 233
NdeII GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
NlaIII CATG 6 cut(s) 181, 293, 440, 563, 815, 1075
NlaIV GGNNCC 7 cut(s) 56, 582, 646, 834, 846, 929, 990
NmuCI GTSAC 2 cut(s) 19, 774
NspI RCATGY 3 cut(s) 293, 563, 1075
NspV TTCGAA 1 cut(s) 1055
PaeI GCATGC 1 cut(s) 1075
PagI TCATGA 1 cut(s) 436
PaqCI CACCTGC 1 cut(s) 880
PctI GAATGC 1 cut(s) 913
PdmI GAANNNNTTC 2 cut(s) 401, 498
PfeI GAWTC 5 cut(s) 130, 169, 318, 797, 872
PflMI CCANNNNNTGG 1 cut(s) 64
PinAI ACCGGT 1 cut(s) 451
PkrI GCNGC 1 cut(s) 900
PspFI CCCAGC 1 cut(s) 143
PspN4I GGNNCC 7 cut(s) 56, 582, 646, 834, 846, 929, 990
PspPI GGNCC 2 cut(s) 54, 928
PstI CTGCAG 1 cut(s) 507
PstNI CAGNNNCTG 1 cut(s) 791
PsuI RGATCY 2 cut(s) 79, 1095
RsaI GTAC 3 cut(s) 103, 176, 194
RsaNI GTAC 3 cut(s) 102, 175, 193
SaqAI TTAA 2 cut(s) 261, 420
SatI GCNGC 1 cut(s) 899
Sau3AI GATC 9 cut(s) 70, 79, 180, 223, 357, 413, 676, 826, 1095
Sau96I GGNCC 2 cut(s) 54, 928
ScrFI CCNGG 1 cut(s) 233
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 5 cut(s) 4, 524, 529, 876, 997
SfcI CTRYAG 2 cut(s) 503, 780
SfuI TTCGAA 1 cut(s) 1055
SinI GGWCC 2 cut(s) 54, 928
SphI GCATGC 1 cut(s) 1075
Sse9I AATT 6 cut(s) 39, 148, 401, 417, 512, 567
SsiI CCGC 1 cut(s) 931
SspMI CTAG 2 cut(s) 731, 1099
StyD4I CCNGG 1 cut(s) 231
TaaI ACNGT 2 cut(s) 270, 862
TaqI TCGA 2 cut(s) 295, 1055
TasI AATT 6 cut(s) 39, 148, 401, 417, 512, 567
TatI WGTACW 1 cut(s) 174
TfiI GAWTC 5 cut(s) 130, 169, 318, 797, 872
Tru1I TTAA 2 cut(s) 261, 420
Tru9I TTAA 2 cut(s) 261, 420
TscAI CASTG 3 cut(s) 691, 792, 865
TseFI GTSAC 2 cut(s) 19, 774
TseI GCWGC 1 cut(s) 898
Tsp45I GTSAC 2 cut(s) 19, 774
TspDTI ATGAA 3 cut(s) 288, 348, 885
TspRI CASTG 3 cut(s) 691, 792, 865
Van91I CCANNNNNTGG 1 cut(s) 64
VpaK11BI GGWCC 2 cut(s) 54, 928
XapI RAATTY 3 cut(s) 148, 401, 567
XceI RCATGY 3 cut(s) 293, 563, 1075
XmnI GAANNNNTTC 2 cut(s) 401, 498
XspI CTAG 2 cut(s) 731, 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.