Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 147 |
| AcuI | CTGAAG | 2 cut(s) | 75, 260 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 130 |
| AgsI | TTSAA | 3 cut(s) | 9, 93, 230 |
| AjiI | CACGTC | 1 cut(s) | 64 |
| Alw26I | GTCTC | 1 cut(s) | 238 |
| AlwI | GGATC | 1 cut(s) | 147 |
| ApeKI | GCWGC | 1 cut(s) | 35 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 81 |
| AspS9I | GGNCC | 1 cut(s) | 133 |
| AsuC2I | CCSGG | 1 cut(s) | 137 |
| AvaII | GGWCC | 1 cut(s) | 133 |
| BbsI | GAAGAC | 1 cut(s) | 105 |
| BbvI | GCAGC | 1 cut(s) | 47 |
| BccI | CCATC | 4 cut(s) | 62, 203, 230, 319 |
| BcnI | CCSGG | 1 cut(s) | 137 |
| BcoDI | GTCTC | 1 cut(s) | 238 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 44 |
| BisI | GCNGC | 1 cut(s) | 36 |
| BlsI | GCNGC | 1 cut(s) | 37 |
| Bme1390I | CCNGG | 1 cut(s) | 137 |
| Bme18I | GGWCC | 1 cut(s) | 133 |
| BmgBI | CACGTC | 1 cut(s) | 64 |
| BmgT120I | GGNCC | 1 cut(s) | 133 |
| BmiI | GGNNCC | 1 cut(s) | 294 |
| BmrFI | CCNGG | 1 cut(s) | 137 |
| BpiI | GAAGAC | 1 cut(s) | 105 |
| BpmI | CTGGAG | 1 cut(s) | 218 |
| BpuMI | CCSGG | 1 cut(s) | 137 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 130 |
| Bse1I | ACTGG | 2 cut(s) | 27, 107 |
| BseGI | GGATG | 4 cut(s) | 73, 258, 273, 306 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 130 |
| BseNI | ACTGG | 2 cut(s) | 27, 107 |
| BseRI | GAGGAG | 2 cut(s) | 215, 270 |
| BseXI | GCAGC | 1 cut(s) | 47 |
| BsiSI | CCGG | 1 cut(s) | 136 |
| BslFI | GGGAC | 1 cut(s) | 74 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 130 |
| BsmAI | GTCTC | 1 cut(s) | 238 |
| BsmFI | GGGAC | 1 cut(s) | 74 |
| Bsp143I | GATC | 1 cut(s) | 139 |
| BspLI | GGNNCC | 1 cut(s) | 294 |
| BspPI | GGATC | 1 cut(s) | 147 |
| BsrI | ACTGG | 2 cut(s) | 27, 107 |
| BssMI | GATC | 1 cut(s) | 139 |
| Bst4CI | ACNGT | 1 cut(s) | 19 |
| BstF5I | GGATG | 4 cut(s) | 73, 258, 273, 306 |
| BstKTI | GATC | 1 cut(s) | 142 |
| BstMAI | GTCTC | 1 cut(s) | 238 |
| BstMBI | GATC | 1 cut(s) | 139 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 32, 44 |
| BstNSI | RCATGY | 1 cut(s) | 248 |
| BstSCI | CCNGG | 1 cut(s) | 135 |
| BstV1I | GCAGC | 1 cut(s) | 47 |
| BstV2I | GAAGAC | 1 cut(s) | 105 |
| BtrI | CACGTC | 1 cut(s) | 64 |
| BtsCI | GGATG | 4 cut(s) | 73, 258, 273, 306 |
| BtsI | GCAGTG | 1 cut(s) | 21 |
| BtsIMutI | CAGTG | 1 cut(s) | 21 |
| Cfr13I | GGNCC | 1 cut(s) | 133 |
| CpoI | CGGWCCG | 1 cut(s) | 133 |
| CspI | CGGWCCG | 1 cut(s) | 133 |
| CviAII | CATG | 1 cut(s) | 245 |
| CviJI | RGCY | 4 cut(s) | 5, 38, 47, 185 |
| CviKI_1 | RGCY | 4 cut(s) | 5, 38, 47, 185 |
| DpnI | GATC | 1 cut(s) | 141 |
| DpnII | GATC | 1 cut(s) | 139 |
| Eco47I | GGWCC | 1 cut(s) | 133 |
| Eco57I | CTGAAG | 2 cut(s) | 75, 260 |
| FaeI | CATG | 1 cut(s) | 248 |
| FaiI | YATR | 3 cut(s) | 193, 207, 246 |
| FaqI | GGGAC | 1 cut(s) | 74 |
| FatI | CATG | 1 cut(s) | 244 |
| Fnu4HI | GCNGC | 1 cut(s) | 36 |
| FokI | GGATG | 4 cut(s) | 80, 260, 265, 293 |
| Fsp4HI | GCNGC | 1 cut(s) | 36 |
| GluI | GCNGC | 1 cut(s) | 36 |
| GsuI | CTGGAG | 1 cut(s) | 218 |
| HapII | CCGG | 1 cut(s) | 136 |
| Hin1II | CATG | 1 cut(s) | 248 |
| HinfI | GANTC | 2 cut(s) | 89, 128 |
| HpaII | CCGG | 1 cut(s) | 136 |
| Hpy188I | TCNGA | 2 cut(s) | 144, 240 |
| Hpy188III | TCNNGA | 4 cut(s) | 86, 93, 197, 338 |
| HpyAV | CCTTC | 2 cut(s) | 50, 71 |
| HpyCH4III | ACNGT | 1 cut(s) | 19 |
| HpyCH4IV | ACGT | 1 cut(s) | 63 |
| HpyCH4V | TGCA | 1 cut(s) | 35 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 32, 44 |
| HpySE526I | ACGT | 1 cut(s) | 63 |
| Hsp92II | CATG | 1 cut(s) | 248 |
| Kzo9I | GATC | 1 cut(s) | 139 |
| Lsp1109I | GCAGC | 1 cut(s) | 47 |
| MaeII | ACGT | 1 cut(s) | 63 |
| MaeIII | GTNAC | 1 cut(s) | 19 |
| MalI | GATC | 1 cut(s) | 141 |
| MboI | GATC | 1 cut(s) | 139 |
| MboII | GAAGA | 2 cut(s) | 110, 209 |
| MluCI | AATT | 1 cut(s) | 301 |
| MnlI | CCTC | 6 cut(s) | 49, 193, 283, 288, 291, 320 |
| MroXI | GAANNNNTTC | 1 cut(s) | 81 |
| MspI | CCGG | 1 cut(s) | 136 |
| MspR9I | CCNGG | 1 cut(s) | 137 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 32, 44 |
| NciI | CCSGG | 1 cut(s) | 137 |
| NdeII | GATC | 1 cut(s) | 139 |
| NlaIII | CATG | 1 cut(s) | 248 |
| NlaIV | GGNNCC | 1 cut(s) | 294 |
| NmuCI | GTSAC | 1 cut(s) | 19 |
| NspI | RCATGY | 1 cut(s) | 248 |
| PdmI | GAANNNNTTC | 1 cut(s) | 81 |
| PfeI | GAWTC | 2 cut(s) | 89, 128 |
| PflFI | GACNNNGTC | 1 cut(s) | 17 |
| PfoI | TCCNGGA | 1 cut(s) | 135 |
| PkrI | GCNGC | 1 cut(s) | 37 |
| PspN4I | GGNNCC | 1 cut(s) | 294 |
| PspPI | GGNCC | 1 cut(s) | 133 |
| PsyI | GACNNNGTC | 1 cut(s) | 17 |
| Rsr2I | CGGWCCG | 1 cut(s) | 133 |
| RsrII | CGGWCCG | 1 cut(s) | 133 |
| SatI | GCNGC | 1 cut(s) | 36 |
| Sau3AI | GATC | 1 cut(s) | 139 |
| Sau96I | GGNCC | 1 cut(s) | 133 |
| ScrFI | CCNGG | 1 cut(s) | 137 |
| SetI | ASST | 5 cut(s) | 66, 112, 153, 298, 324 |
| SinI | GGWCC | 1 cut(s) | 133 |
| Sse9I | AATT | 1 cut(s) | 301 |
| StyD4I | CCNGG | 1 cut(s) | 135 |
| TaaI | ACNGT | 1 cut(s) | 19 |
| TaiI | ACGT | 1 cut(s) | 66 |
| TaqI | TCGA | 1 cut(s) | 12 |
| TaqII | GACCGA | 1 cut(s) | 121 |
| TasI | AATT | 1 cut(s) | 301 |
| TfiI | GAWTC | 2 cut(s) | 89, 128 |
| TscAI | CASTG | 1 cut(s) | 28 |
| TseFI | GTSAC | 1 cut(s) | 19 |
| TseI | GCWGC | 1 cut(s) | 35 |
| Tsp45I | GTSAC | 1 cut(s) | 19 |
| TspDTI | ATGAA | 1 cut(s) | 295 |
| TspRI | CASTG | 1 cut(s) | 28 |
| Tth111I | GACNNNGTC | 1 cut(s) | 17 |
| VpaK11BI | GGWCC | 1 cut(s) | 133 |
| XceI | RCATGY | 1 cut(s) | 248 |
| XmnI | GAANNNNTTC | 1 cut(s) | 81 |