No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 495 |
| Acc36I | ACCTGC | 1 cut(s) | 2259 |
| AccB1I | GGYRCC | 1 cut(s) | 2307 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 908 |
| AccBSI | CCGCTC | 1 cut(s) | 1412 |
| AciI | CCGC | 8 cut(s) | 327, 336, 363, 829, 1410, 2392, 2525, 2555 |
| AcoI | YGGCCR | 1 cut(s) | 1123 |
| AcsI | RAATTY | 4 cut(s) | 250, 620, 669, 2585 |
| AcyI | GRCGYC | 1 cut(s) | 2308 |
| AfaI | GTAC | 6 cut(s) | 118, 1578, 1767, 1847, 1856, 2456 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 368, 369, 879, 908, 948, 949, 1138, 2130 |
| AgsI | TTSAA | 9 cut(s) | 78, 406, 734, 805, 1220, 2216, 2278, 2423, 2548 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1942 |
| AjnI | CCWGG | 2 cut(s) | 1056, 1334 |
| AjuI | GAANNNNNNNTTGG | 3 cut(s) | 2622, 2654, 2868 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 96, 128, 1316, 1348 |
| AluBI | AGCT | 4 cut(s) | 1144, 1150, 1421, 2800 |
| AluI | AGCT | 4 cut(s) | 1144, 1150, 1421, 2800 |
| Alw26I | GTCTC | 4 cut(s) | 481, 589, 1248, 1950 |
| Ama87I | CYCGRG | 1 cut(s) | 138 |
| AoxI | GGCC | 6 cut(s) | 1123, 1605, 1749, 2020, 2120, 2342 |
| ApoI | RAATTY | 4 cut(s) | 250, 620, 669, 2585 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1226, 1258 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 254, 1052, 1532 |
| AspLEI | GCGC | 3 cut(s) | 1171, 2048, 2310 |
| AspS9I | GGNCC | 8 cut(s) | 359, 1554, 1606, 1749, 1955, 2121, 2343, 2518 |
| AsuC2I | CCSGG | 4 cut(s) | 741, 1953, 2065, 2870 |
| AsuHPI | GGTGA | 4 cut(s) | 15, 563, 617, 1204 |
| AsuII | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| AvaI | CYCGRG | 1 cut(s) | 138 |
| AvaII | GGWCC | 4 cut(s) | 359, 1554, 1955, 2518 |
| BamHI | GGATCC | 4 cut(s) | 1405, 1725, 2057, 2527 |
| BanI | GGYRCC | 1 cut(s) | 2307 |
| BanII | GRGCYC | 1 cut(s) | 2296 |
| BauI | CACGAG | 1 cut(s) | 2463 |
| BccI | CCATC | 7 cut(s) | 247, 464, 910, 2408, 2712, 2779, 2853 |
| BceAI | ACGGC | 2 cut(s) | 1110, 1736 |
| BciT130I | CCWGG | 2 cut(s) | 1058, 1336 |
| BciVI | GTATCC | 1 cut(s) | 243 |
| BclI | TGATCA | 3 cut(s) | 664, 694, 2176 |
| BcnI | CCSGG | 4 cut(s) | 741, 1953, 2065, 2870 |
| BcoDI | GTCTC | 4 cut(s) | 481, 589, 1248, 1950 |
| BfaI | CTAG | 8 cut(s) | 278, 308, 948, 1007, 1359, 1506, 2012, 2298 |
| BfmI | CTRYAG | 2 cut(s) | 145, 972 |
| BfoI | RGCGCY | 2 cut(s) | 2049, 2311 |
| BfuAI | ACCTGC | 1 cut(s) | 2259 |
| BfuI | GTATCC | 1 cut(s) | 243 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 2316, 2614 |
| BglII | AGATCT | 5 cut(s) | 513, 1582, 1661, 2471, 2635 |
| BisI | GCNGC | 1 cut(s) | 337 |
| BlsI | GCNGC | 1 cut(s) | 338 |
| Bme1390I | CCNGG | 6 cut(s) | 741, 1058, 1336, 1953, 2065, 2870 |
| Bme18I | GGWCC | 4 cut(s) | 359, 1554, 1955, 2518 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1942 |
| BmeT110I | CYCGRG | 1 cut(s) | 138 |
| BmgT120I | GGNCC | 8 cut(s) | 359, 1554, 1606, 1749, 1955, 2121, 2343, 2518 |
| BmrFI | CCNGG | 6 cut(s) | 741, 1058, 1336, 1953, 2065, 2870 |
| BmsI | GCATC | 3 cut(s) | 1176, 2139, 2595 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 670, 702 |
| BpmI | CTGGAG | 2 cut(s) | 1166, 1318 |
| Bpu14I | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| BpuEI | CTTGAG | 1 cut(s) | 1917 |
| BpuMI | CCSGG | 4 cut(s) | 741, 1953, 2065, 2870 |
| Bsa29I | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| BsaBI | GATNNNNATC | 3 cut(s) | 231, 2007, 2097 |
| BsaHI | GRCGYC | 1 cut(s) | 2308 |
| BsaI | GGTCTC | 2 cut(s) | 589, 1950 |
| BsaJI | CCNNGG | 6 cut(s) | 1071, 1309, 1335, 1563, 2129, 2312 |
| BsaWI | WCCGGW | 1 cut(s) | 2050 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1316, 1346, 2226, 2256 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 368, 369, 879, 908, 948, 949, 1138, 2130 |
| Bse1I | ACTGG | 1 cut(s) | 985 |
| Bse3DI | GCAATG | 2 cut(s) | 1245, 2108 |
| Bse8I | GATNNNNATC | 3 cut(s) | 231, 2007, 2097 |
| BseBI | CCWGG | 2 cut(s) | 1058, 1336 |
| BseCI | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| BseDI | CCNNGG | 6 cut(s) | 1071, 1309, 1335, 1563, 2129, 2312 |
| BseGI | GGATG | 5 cut(s) | 809, 1288, 2419, 2771, 2878 |
| BseJI | GATNNNNATC | 3 cut(s) | 231, 2007, 2097 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 368, 369, 879, 908, 948, 949, 1138, 2130 |
| BseMI | GCAATG | 2 cut(s) | 1245, 2108 |
| BseMII | CTCAG | 1 cut(s) | 35 |
| BseNI | ACTGG | 1 cut(s) | 985 |
| BseRI | GAGGAG | 1 cut(s) | 2279 |
| BseX3I | CGGCCG | 1 cut(s) | 1123 |
| Bsh1285I | CGRYCG | 2 cut(s) | 1126, 1929 |
| BshFI | GGCC | 6 cut(s) | 1125, 1607, 1751, 2022, 2122, 2344 |
| BshNI | GGYRCC | 1 cut(s) | 2307 |
| BshVI | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| BsiEI | CGRYCG | 2 cut(s) | 1126, 1929 |
| BsiHKCI | CYCGRG | 1 cut(s) | 138 |
| BsiSI | CCGG | 7 cut(s) | 740, 1190, 1920, 1953, 2051, 2065, 2870 |
| BslFI | GGGAC | 2 cut(s) | 345, 2531 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 368, 369, 879, 908, 948, 949, 1138, 2130 |
| BsmAI | GTCTC | 4 cut(s) | 481, 589, 1248, 1950 |
| BsmFI | GGGAC | 2 cut(s) | 345, 2531 |
| BsmI | GAATGC | 1 cut(s) | 1171 |
| BsnI | GGCC | 6 cut(s) | 1125, 1607, 1751, 2022, 2122, 2344 |
| Bso31I | GGTCTC | 2 cut(s) | 589, 1950 |
| BsoBI | CYCGRG | 1 cut(s) | 138 |
| Bsp119I | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2296 |
| Bsp1407I | TGTACA | 1 cut(s) | 1845 |
| Bsp19I | CCATGG | 1 cut(s) | 1563 |
| BspACI | CCGC | 8 cut(s) | 327, 336, 363, 829, 1410, 2392, 2525, 2555 |
| BspANI | GGCC | 6 cut(s) | 1125, 1607, 1751, 2022, 2122, 2344 |
| BspCNI | CTCAG | 1 cut(s) | 36 |
| BspDI | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| BspHI | TCATGA | 1 cut(s) | 1273 |
| BspMI | ACCTGC | 1 cut(s) | 2259 |
| BspQI | GCTCTTC | 1 cut(s) | 1674 |
| BspT104I | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| BspT107I | GGYRCC | 1 cut(s) | 2307 |
| BspTNI | GGTCTC | 2 cut(s) | 589, 1950 |
| BsrBI | CCGCTC | 1 cut(s) | 1412 |
| BsrDI | GCAATG | 2 cut(s) | 1245, 2108 |
| BsrGI | TGTACA | 1 cut(s) | 1845 |
| BsrI | ACTGG | 1 cut(s) | 985 |
| BssECI | CCNNGG | 6 cut(s) | 1071, 1309, 1335, 1563, 2129, 2312 |
| BssNI | GRCGYC | 1 cut(s) | 2308 |
| BssSI | CACGAG | 1 cut(s) | 2463 |
| BssT1I | CCWWGG | 4 cut(s) | 1071, 1563, 2129, 2312 |
| Bst2BI | CACGAG | 1 cut(s) | 2463 |
| Bst2UI | CCWGG | 2 cut(s) | 1058, 1336 |
| Bst4CI | ACNGT | 4 cut(s) | 551, 593, 1039, 2735 |
| Bst6I | CTCTTC | 2 cut(s) | 1674, 2595 |
| BstACI | GRCGYC | 1 cut(s) | 2308 |
| BstAUI | TGTACA | 1 cut(s) | 1845 |
| BstBI | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| BstC8I | GCNNGC | 1 cut(s) | 2390 |
| BstDEI | CTNAG | 7 cut(s) | 44, 390, 494, 763, 1772, 2140, 2723 |
| BstDSI | CCRYGG | 1 cut(s) | 1563 |
| BstF5I | GGATG | 5 cut(s) | 809, 1288, 2419, 2771, 2878 |
| BstH2I | RGCGCY | 2 cut(s) | 2049, 2311 |
| BstHHI | GCGC | 3 cut(s) | 1171, 2048, 2310 |
| BstMAI | GTCTC | 4 cut(s) | 481, 589, 1248, 1950 |
| BstMCI | CGRYCG | 2 cut(s) | 1126, 1929 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 1418, 2119, 2316, 2614, 2686 |
| BstNI | CCWGG | 2 cut(s) | 1058, 1336 |
| BstNSI | RCATGY | 2 cut(s) | 346, 1443 |
| BstSCI | CCNGG | 6 cut(s) | 739, 1056, 1334, 1951, 2063, 2868 |
| BstSFI | CTRYAG | 2 cut(s) | 145, 972 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 1402, 1564, 2353 |
| BstZI | CGGCCG | 1 cut(s) | 1123 |
| Bsu15I | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| BsuI | GTATCC | 1 cut(s) | 243 |
| BsuRI | GGCC | 6 cut(s) | 1125, 1607, 1751, 2022, 2122, 2344 |
| BsuTUI | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| BtgI | CCRYGG | 1 cut(s) | 1563 |
| BtgZI | GCGATG | 1 cut(s) | 2693 |
| BtsCI | GGATG | 5 cut(s) | 809, 1288, 2419, 2771, 2878 |
| BtsIMutI | CAGTG | 1 cut(s) | 598 |
| BveI | ACCTGC | 1 cut(s) | 2259 |
| Cac8I | GCNNGC | 1 cut(s) | 2390 |
| CciI | TCATGA | 1 cut(s) | 1273 |
| CfoI | GCGC | 3 cut(s) | 1171, 2048, 2310 |
| Cfr13I | GGNCC | 8 cut(s) | 359, 1554, 1606, 1749, 1955, 2121, 2343, 2518 |
| ClaI | ATCGAT | 3 cut(s) | 420, 429, 1613 |
| Csp6I | GTAC | 6 cut(s) | 117, 1577, 1766, 1846, 1855, 2455 |
| CviQI | GTAC | 6 cut(s) | 117, 1577, 1766, 1846, 1855, 2455 |
| DdeI | CTNAG | 7 cut(s) | 44, 390, 494, 763, 1772, 2140, 2723 |
| DinI | GGCGCC | 1 cut(s) | 2309 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 495 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1942 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 495 |
| EaeI | YGGCCR | 1 cut(s) | 1123 |
| EagI | CGGCCG | 1 cut(s) | 1123 |
| Eam1104I | CTCTTC | 2 cut(s) | 1674, 2595 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1942 |
| EarI | CTCTTC | 2 cut(s) | 1674, 2595 |
| EciI | GGCGGA | 2 cut(s) | 844, 2544 |
| EclXI | CGGCCG | 1 cut(s) | 1123 |
| Eco130I | CCWWGG | 4 cut(s) | 1071, 1563, 2129, 2312 |
| Eco24I | GRGCYC | 1 cut(s) | 2296 |
| Eco31I | GGTCTC | 2 cut(s) | 589, 1950 |
| Eco32I | GATATC | 4 cut(s) | 33, 107, 424, 1120 |
| Eco47I | GGWCC | 4 cut(s) | 359, 1554, 1955, 2518 |
| Eco52I | CGGCCG | 1 cut(s) | 1123 |
| Eco88I | CYCGRG | 1 cut(s) | 138 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1606, 2518 |
| EcoRI | GAATTC | 3 cut(s) | 250, 620, 2585 |
| EcoRII | CCWGG | 2 cut(s) | 1056, 1334 |
| EcoRV | GATATC | 4 cut(s) | 33, 107, 424, 1120 |
| EcoT14I | CCWWGG | 4 cut(s) | 1071, 1563, 2129, 2312 |
| EcoT22I | ATGCAT | 1 cut(s) | 1445 |
| EcoT38I | GRGCYC | 1 cut(s) | 2296 |
| EgeI | GGCGCC | 1 cut(s) | 2309 |
| EheI | GGCGCC | 1 cut(s) | 2309 |
| ErhI | CCWWGG | 4 cut(s) | 1071, 1563, 2129, 2312 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 257, 289 |
| FaqI | GGGAC | 2 cut(s) | 345, 2531 |
| FauI | CCCGC | 2 cut(s) | 370, 1417 |
| FbaI | TGATCA | 3 cut(s) | 664, 694, 2176 |
| Fnu4HI | GCNGC | 1 cut(s) | 337 |
| FokI | GGATG | 5 cut(s) | 796, 1295, 2426, 2758, 2885 |
| FriOI | GRGCYC | 1 cut(s) | 2296 |
| Fsp4HI | GCNGC | 1 cut(s) | 337 |
| FspBI | CTAG | 8 cut(s) | 278, 308, 948, 1007, 1359, 1506, 2012, 2298 |
| GlaI | GCGC | 3 cut(s) | 1170, 2047, 2309 |
| GluI | GCNGC | 1 cut(s) | 337 |
| GsuI | CTGGAG | 2 cut(s) | 1166, 1318 |
| HaeII | RGCGCY | 2 cut(s) | 2049, 2311 |
| HaeIII | GGCC | 6 cut(s) | 1125, 1607, 1751, 2022, 2122, 2344 |
| HapII | CCGG | 7 cut(s) | 740, 1190, 1920, 1953, 2051, 2065, 2870 |
| HhaI | GCGC | 3 cut(s) | 1171, 2048, 2310 |
| Hin1I | GRCGYC | 1 cut(s) | 2308 |
| Hin6I | GCGC | 3 cut(s) | 1169, 2046, 2308 |
| HinP1I | GCGC | 3 cut(s) | 1169, 2046, 2308 |
| HincII | GTYRAC | 1 cut(s) | 769 |
| HindII | GTYRAC | 1 cut(s) | 769 |
| HpaII | CCGG | 7 cut(s) | 740, 1190, 1920, 1953, 2051, 2065, 2870 |
| HphI | GGTGA | 4 cut(s) | 15, 563, 617, 1204 |
| Hpy166II | GTNNAC | 4 cut(s) | 119, 547, 769, 1324 |
| Hpy8I | GTNNAC | 4 cut(s) | 119, 547, 769, 1324 |
| HpyCH4III | ACNGT | 4 cut(s) | 551, 593, 1039, 2735 |
| HpyCH4IV | ACGT | 2 cut(s) | 385, 543 |
| HpyCH4V | TGCA | 8 cut(s) | 1251, 1443, 1757, 2082, 2152, 2254, 2446, 2594 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 1418, 2119, 2316, 2614, 2686 |
| HpyF3I | CTNAG | 7 cut(s) | 44, 390, 494, 763, 1772, 2140, 2723 |
| HpySE526I | ACGT | 2 cut(s) | 385, 543 |
| Hsp92I | GRCGYC | 1 cut(s) | 2308 |
| HspAI | GCGC | 3 cut(s) | 1169, 2046, 2308 |
| KasI | GGCGCC | 1 cut(s) | 2307 |
| KflI | GGGWCCC | 1 cut(s) | 2518 |
| Ksp22I | TGATCA | 3 cut(s) | 664, 694, 2176 |
| LguI | GCTCTTC | 1 cut(s) | 1674 |
| LmnI | GCTCC | 8 cut(s) | 743, 930, 1141, 1147, 1426, 1787, 2053, 2299 |
| LweI | GCATC | 3 cut(s) | 1176, 2139, 2595 |
| MaeI | CTAG | 8 cut(s) | 278, 308, 948, 1007, 1359, 1506, 2012, 2298 |
| MaeII | ACGT | 2 cut(s) | 385, 543 |
| MaeIII | GTNAC | 7 cut(s) | 21, 153, 1094, 1112, 1127, 1823, 2104 |
| MbiI | CCGCTC | 1 cut(s) | 1412 |
| MhlI | GDGCHC | 1 cut(s) | 2296 |
| Mly113I | GGCGCC | 1 cut(s) | 2308 |
| MlyI | GAGTC | 9 cut(s) | 29, 679, 688, 707, 1301, 1378, 1983, 2250, 2458 |
| Mph1103I | ATGCAT | 1 cut(s) | 1445 |
| MroXI | GAANNNNTTC | 3 cut(s) | 254, 1052, 1532 |
| MseI | TTAA | 5 cut(s) | 263, 644, 1433, 1446, 1886 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1562 |
| MspI | CCGG | 7 cut(s) | 740, 1190, 1920, 1953, 2051, 2065, 2870 |
| MspR9I | CCNGG | 6 cut(s) | 741, 1058, 1336, 1953, 2065, 2870 |
| Mva1269I | GAATGC | 1 cut(s) | 1171 |
| MvaI | CCWGG | 2 cut(s) | 1058, 1336 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 1418, 2119, 2316, 2614, 2686 |
| NarI | GGCGCC | 1 cut(s) | 2308 |
| NciI | CCSGG | 4 cut(s) | 741, 1953, 2065, 2870 |
| NcoI | CCATGG | 1 cut(s) | 1563 |
| NmuCI | GTSAC | 5 cut(s) | 21, 1094, 1112, 1127, 2104 |
| NsiI | ATGCAT | 1 cut(s) | 1445 |
| NspI | RCATGY | 2 cut(s) | 346, 1443 |
| NspV | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| PaeR7I | CTCGAG | 1 cut(s) | 138 |
| PagI | TCATGA | 1 cut(s) | 1273 |
| PciSI | GCTCTTC | 1 cut(s) | 1674 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 426, 482, 584 |
| PctI | GAATGC | 1 cut(s) | 1171 |
| PdmI | GAANNNNTTC | 3 cut(s) | 254, 1052, 1532 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 908 |
| PfoI | TCCNGGA | 3 cut(s) | 1056, 2063, 2868 |
| PkrI | GCNGC | 1 cut(s) | 338 |
| PleI | GAGTC | 9 cut(s) | 28, 679, 687, 707, 1300, 1378, 1983, 2249, 2457 |
| PluTI | GGCGCC | 1 cut(s) | 2311 |
| PpsI | GAGTC | 9 cut(s) | 28, 679, 687, 707, 1300, 1378, 1983, 2249, 2457 |
| PpuMI | RGGWCCY | 1 cut(s) | 2518 |
| Psp5II | RGGWCCY | 1 cut(s) | 2518 |
| Psp6I | CCWGG | 2 cut(s) | 1056, 1334 |
| PspGI | CCWGG | 2 cut(s) | 1056, 1334 |
| PspPI | GGNCC | 8 cut(s) | 359, 1554, 1606, 1749, 1955, 2121, 2343, 2518 |
| PspPPI | RGGWCCY | 1 cut(s) | 2518 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1695, 1727 |
| RsaI | GTAC | 6 cut(s) | 118, 1578, 1767, 1847, 1856, 2456 |
| RsaNI | GTAC | 6 cut(s) | 117, 1577, 1766, 1846, 1855, 2455 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1562 |
| SapI | GCTCTTC | 1 cut(s) | 1674 |
| SaqAI | TTAA | 5 cut(s) | 263, 644, 1433, 1446, 1886 |
| SatI | GCNGC | 1 cut(s) | 337 |
| Sau96I | GGNCC | 8 cut(s) | 359, 1554, 1606, 1749, 1955, 2121, 2343, 2518 |
| SchI | GAGTC | 9 cut(s) | 29, 679, 688, 707, 1301, 1378, 1983, 2250, 2458 |
| ScrFI | CCNGG | 6 cut(s) | 741, 1058, 1336, 1953, 2065, 2870 |
| SduI | GDGCHC | 1 cut(s) | 2296 |
| SfaNI | GCATC | 3 cut(s) | 1176, 2139, 2595 |
| SfcI | CTRYAG | 2 cut(s) | 145, 972 |
| SfoI | GGCGCC | 1 cut(s) | 2309 |
| Sfr274I | CTCGAG | 1 cut(s) | 138 |
| SfuI | TTCGAA | 5 cut(s) | 168, 1224, 1860, 2497, 2694 |
| SinI | GGWCC | 4 cut(s) | 359, 1554, 1955, 2518 |
| SlaI | CTCGAG | 1 cut(s) | 138 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1562 |
| SmlI | CTYRAG | 2 cut(s) | 138, 1932 |
| SmoI | CTYRAG | 2 cut(s) | 138, 1932 |
| SsiI | CCGC | 8 cut(s) | 327, 336, 363, 829, 1410, 2392, 2525, 2555 |
| SspDI | GGCGCC | 1 cut(s) | 2307 |
| SspI | AATATT | 2 cut(s) | 401, 1366 |
| SspMI | CTAG | 8 cut(s) | 278, 308, 948, 1007, 1359, 1506, 2012, 2298 |
| StyD4I | CCNGG | 6 cut(s) | 739, 1056, 1334, 1951, 2063, 2868 |
| StyI | CCWWGG | 4 cut(s) | 1071, 1563, 2129, 2312 |
| TaaI | ACNGT | 4 cut(s) | 551, 593, 1039, 2735 |
| TaiI | ACGT | 2 cut(s) | 388, 546 |
| TaqII | GACCGA | 2 cut(s) | 1215, 1915 |
| TatI | WGTACW | 2 cut(s) | 1765, 1845 |
| TauI | GCSGC | 1 cut(s) | 339 |
| Tru1I | TTAA | 5 cut(s) | 263, 644, 1433, 1446, 1886 |
| Tru9I | TTAA | 5 cut(s) | 263, 644, 1433, 1446, 1886 |
| TscAI | CASTG | 1 cut(s) | 598 |
| TseFI | GTSAC | 5 cut(s) | 21, 1094, 1112, 1127, 2104 |
| Tsp45I | GTSAC | 5 cut(s) | 21, 1094, 1112, 1127, 2104 |
| TspGWI | ACGGA | 5 cut(s) | 183, 917, 1154, 2272, 2747 |
| TspRI | CASTG | 1 cut(s) | 598 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 908 |
| VpaK11BI | GGWCC | 4 cut(s) | 359, 1554, 1955, 2518 |
| XapI | RAATTY | 4 cut(s) | 250, 620, 669, 2585 |
| XbaI | TCTAGA | 1 cut(s) | 307 |
| XceI | RCATGY | 2 cut(s) | 346, 1443 |
| XhoI | CTCGAG | 1 cut(s) | 138 |
| XmnI | GAANNNNTTC | 3 cut(s) | 254, 1052, 1532 |
| XspI | CTAG | 8 cut(s) | 278, 308, 948, 1007, 1359, 1506, 2012, 2298 |
| Zsp2I | ATGCAT | 1 cut(s) | 1445 |