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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 84 |
| AclWI | GGATC | 3 cut(s) | 4, 32, 202 |
| AcoI | YGGCCR | 1 cut(s) | 57 |
| AcsI | RAATTY | 2 cut(s) | 22, 175 |
| AgsI | TTSAA | 1 cut(s) | 116 |
| AlwI | GGATC | 3 cut(s) | 4, 32, 202 |
| AoxI | GGCC | 2 cut(s) | 57, 154 |
| ApoI | RAATTY | 2 cut(s) | 22, 175 |
| AspS9I | GGNCC | 1 cut(s) | 155 |
| BalI | TGGCCA | 1 cut(s) | 59 |
| BmgT120I | GGNCC | 1 cut(s) | 155 |
| BmiI | GGNNCC | 1 cut(s) | 157 |
| Bsa29I | ATCGAT | 1 cut(s) | 88 |
| BsaBI | GATNNNNATC | 1 cut(s) | 8 |
| Bse8I | GATNNNNATC | 1 cut(s) | 8 |
| BseCI | ATCGAT | 1 cut(s) | 88 |
| BseJI | GATNNNNATC | 1 cut(s) | 8 |
| Bsh1236I | CGCG | 1 cut(s) | 84 |
| Bsh1285I | CGRYCG | 1 cut(s) | 88 |
| BshFI | GGCC | 2 cut(s) | 59, 156 |
| BshVI | ATCGAT | 1 cut(s) | 88 |
| BsiEI | CGRYCG | 1 cut(s) | 88 |
| BsiSI | CCGG | 1 cut(s) | 153 |
| BsmI | GAATGC | 1 cut(s) | 141 |
| BsnI | GGCC | 2 cut(s) | 59, 156 |
| Bsp143I | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| BspANI | GGCC | 2 cut(s) | 59, 156 |
| BspDI | ATCGAT | 1 cut(s) | 88 |
| BspFNI | CGCG | 1 cut(s) | 84 |
| BspLI | GGNNCC | 1 cut(s) | 157 |
| BspPI | GGATC | 3 cut(s) | 4, 32, 202 |
| BssMI | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| Bst4CI | ACNGT | 1 cut(s) | 64 |
| Bst6I | CTCTTC | 1 cut(s) | 99 |
| BstC8I | GCNNGC | 1 cut(s) | 76 |
| BstFNI | CGCG | 1 cut(s) | 84 |
| BstKTI | GATC | 5 cut(s) | 12, 40, 88, 92, 197 |
| BstMBI | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| BstMCI | CGRYCG | 1 cut(s) | 88 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 56 |
| BstUI | CGCG | 1 cut(s) | 84 |
| BstX2I | RGATCY | 2 cut(s) | 9, 194 |
| BstYI | RGATCY | 2 cut(s) | 9, 194 |
| Bsu15I | ATCGAT | 1 cut(s) | 88 |
| BsuRI | GGCC | 2 cut(s) | 59, 156 |
| BsuTUI | ATCGAT | 1 cut(s) | 88 |
| BtsIMutI | CAGTG | 1 cut(s) | 60 |
| Cac8I | GCNNGC | 1 cut(s) | 76 |
| Cfr13I | GGNCC | 1 cut(s) | 155 |
| ClaI | ATCGAT | 1 cut(s) | 88 |
| CviJI | RGCY | 2 cut(s) | 59, 156 |
| CviKI_1 | RGCY | 2 cut(s) | 59, 156 |
| DpnI | GATC | 5 cut(s) | 11, 39, 87, 91, 196 |
| DpnII | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| DraI | TTTAAA | 1 cut(s) | 174 |
| EaeI | YGGCCR | 1 cut(s) | 57 |
| Eam1104I | CTCTTC | 1 cut(s) | 99 |
| EarI | CTCTTC | 1 cut(s) | 99 |
| FaiI | YATR | 4 cut(s) | 52, 187, 205, 209 |
| HaeIII | GGCC | 2 cut(s) | 59, 156 |
| HapII | CCGG | 1 cut(s) | 153 |
| HpaII | CCGG | 1 cut(s) | 153 |
| Hpy99I | CGWCG | 1 cut(s) | 104 |
| HpyCH4III | ACNGT | 1 cut(s) | 64 |
| HpyCH4V | TGCA | 2 cut(s) | 50, 78 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 56 |
| Kzo9I | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| LpnPI | CCDG | 4 cut(s) | 26, 116, 166, 177 |
| MalI | GATC | 5 cut(s) | 11, 39, 87, 91, 196 |
| MboI | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| MboII | GAAGA | 4 cut(s) | 19, 116, 128, 189 |
| MflI | RGATCY | 2 cut(s) | 9, 194 |
| MlsI | TGGCCA | 1 cut(s) | 59 |
| MluCI | AATT | 3 cut(s) | 22, 95, 175 |
| MluNI | TGGCCA | 1 cut(s) | 59 |
| MnlI | CCTC | 2 cut(s) | 12, 138 |
| Mox20I | TGGCCA | 1 cut(s) | 59 |
| MscI | TGGCCA | 1 cut(s) | 59 |
| MseI | TTAA | 2 cut(s) | 30, 173 |
| Msp20I | TGGCCA | 1 cut(s) | 59 |
| MspI | CCGG | 1 cut(s) | 153 |
| Mva1269I | GAATGC | 1 cut(s) | 141 |
| MvnI | CGCG | 1 cut(s) | 84 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 56 |
| NdeII | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| NlaIV | GGNNCC | 1 cut(s) | 157 |
| PctI | GAATGC | 1 cut(s) | 141 |
| Ple19I | CGATCG | 1 cut(s) | 88 |
| PspN4I | GGNNCC | 1 cut(s) | 157 |
| PspPI | GGNCC | 1 cut(s) | 155 |
| PsuI | RGATCY | 2 cut(s) | 9, 194 |
| PvuI | CGATCG | 1 cut(s) | 88 |
| SaqAI | TTAA | 2 cut(s) | 30, 173 |
| Sau3AI | GATC | 5 cut(s) | 9, 37, 85, 89, 194 |
| Sau96I | GGNCC | 1 cut(s) | 155 |
| SetI | ASST | 1 cut(s) | 130 |
| SgeI | CNNG | 8 cut(s) | 25, 59, 87, 95, 143, 165, 170, 204 |
| SmiI | ATTTAAAT | 1 cut(s) | 174 |
| Sse9I | AATT | 3 cut(s) | 22, 95, 175 |
| SwaI | ATTTAAAT | 1 cut(s) | 174 |
| TaaI | ACNGT | 1 cut(s) | 64 |
| TaqI | TCGA | 2 cut(s) | 88, 102 |
| TasI | AATT | 3 cut(s) | 22, 95, 175 |
| Tru1I | TTAA | 2 cut(s) | 30, 173 |
| Tru9I | TTAA | 2 cut(s) | 30, 173 |
| TscAI | CASTG | 1 cut(s) | 67 |
| TspDTI | ATGAA | 2 cut(s) | 20, 189 |
| TspRI | CASTG | 1 cut(s) | 67 |
| XapI | RAATTY | 2 cut(s) | 22, 175 |