Rmu_sc0002489.1_g000039

Plant mobile domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002489.1
OG0000214
Physical Location & Seq
Forward (+)
135824 .. 136729
906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002489.1_g000039.1.cds

Sequence Viewer

Length: 819 bp
atgagtgttcggtgtttgagtcacaccggagaagagagagagagactgatggggtttggtgaaggtgataatcaagctagcctgggcccttcccctcgctacccattgccagatgatgccatggattactatgcgcttccgatcagtcgacccatcgctgctcttcgatggacgcctggtgattttagcagttgggggccaagaaggaaagtgggtcactggcctaccgccacccccgaggagagaacttggtatcaggaggttcgagcgagagacctggctcgttgggaggcggtgggtataacccaaaccatcgatctgtgctttcgccttcccaaaaacaccaatcctgcaccgctagccgccgctttttgcttctggaatacttccagcaacaccttcgatttcaaattcgggcaaatgggcatcactctactggatattctcgctatcacaagactacccatcgactttgagccttatgtgcatggtcaatttgatagcattcaattccctctgcgaatggaacaggttgaccggacacacctcagcaaatcttatgtatcgtggctcgaatttttctgcaattggcatgaacagacaggcggaatcgctttccttgagttctggctctgccttggccaacctatgttgggtgcattataccgctccctttatcaagttgtaatgcgcccgtttgacacctacattactggccccttctgggtcctcgccttctggctacagatctatttcccttgctttcgtcgcgatgacattccaaaggatctacccggtgattcccttttgggggattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

272

Amino Acids

31.46

Weight (kDa)

5.42

Isoelectric Point (pI)

39.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000214)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0079611
rosa_multiflora Rmu_co8150358.1_g000001 Rmu_co8306601.1_g000001 Rmu_sc0000032.1_g000018 Rmu_sc0000110.1_g000025 Rmu_sc0000110.1_g000026 Rmu_sc0000126.1_g000018 Rmu_sc0000147.1_g000058 Rmu_sc0000187.1_g000010 Rmu_sc0000240.1_g000061 Rmu_sc0000240.1_g000062 Rmu_sc0000258.1_g000018 Rmu_sc0000258.1_g000020 Rmu_sc0000276.1_g000075 Rmu_sc0000276.1_g000076 Rmu_sc0000356.1_g000007 Rmu_sc0000360.1_g000033 Rmu_sc0000368.1_g000014 Rmu_sc0000368.1_g000026 Rmu_sc0000389.1_g000011 Rmu_sc0000588.1_g000024 Rmu_sc0000595.1_g000060 Rmu_sc0000744.1_g000144 Rmu_sc0000744.1_g000145 Rmu_sc0000770.1_g000053 Rmu_sc0000795.1_g000096 Rmu_sc0000810.1_g000011 Rmu_sc0000935.1_g000066 Rmu_sc0000945.1_g000014 Rmu_sc0000962.1_g000011 Rmu_sc0000962.1_g000023 Rmu_sc0000962.1_g000024 Rmu_sc0001047.1_g000012 Rmu_sc0001094.1_g000015 Rmu_sc0001097.1_g000032 Rmu_sc0001254.1_g000006 Rmu_sc0001264.1_g000026 Rmu_sc0001322.1_g000013 Rmu_sc0001373.1_g000001 Rmu_sc0001424.1_g000002 Rmu_sc0001455.1_g000009 Rmu_sc0001461.1_g000008 Rmu_sc0001461.1_g000058 Rmu_sc0001563.1_g000014 Rmu_sc0001793.1_g000012 Rmu_sc0001793.1_g000013 Rmu_sc0001796.1_g000010 Rmu_sc0001817.1_g000004 Rmu_sc0002141.1_g000011 Rmu_sc0002141.1_g000012 Rmu_sc0002183.1_g000010 Rmu_sc0002183.1_g000015 Rmu_sc0002212.1_g000033 Rmu_sc0002212.1_g000034 Rmu_sc0002263.1_g000049 Rmu_sc0002263.1_g000050 Rmu_sc0002263.1_g000055 Rmu_sc0002263.1_g000056 Rmu_sc0002489.1_g000039 Rmu_sc0002654.1_g000003 Rmu_sc0002680.1_g000036 Rmu_sc0002745.1_g000013 Rmu_sc0002751.1_g000001 Rmu_sc0002769.1_g000013 Rmu_sc0002972.1_g000004 Rmu_sc0002985.1_g000011 Rmu_sc0003199.1_g000002 Rmu_sc0003207.1_g000020 Rmu_sc0003402.1_g000005 Rmu_sc0003500.1_g000009 Rmu_sc0003500.1_g000010 Rmu_sc0003600.1_g000051 Rmu_sc0003809.1_g000025 Rmu_sc0003859.1_g000029 Rmu_sc0003859.1_g000030 Rmu_sc0003994.1_g000025 Rmu_sc0004085.1_g000010 Rmu_sc0004085.1_g000015 Rmu_sc0004165.1_g000061 Rmu_sc0004525.1_g000006 Rmu_sc0004549.1_g000012 Rmu_sc0004598.1_g000035 Rmu_sc0004835.1_g000007 Rmu_sc0004881.1_g000008 Rmu_sc0005325.1_g000004 Rmu_sc0005325.1_g000006 Rmu_sc0005608.1_g000003 Rmu_sc0005747.1_g000026 Rmu_sc0006153.1_g000059 Rmu_sc0006273.1_g000010 Rmu_sc0006737.1_g000002 Rmu_sc0006737.1_g000003 Rmu_sc0006984.1_g000006 Rmu_sc0006984.1_g000007 Rmu_sc0007170.1_g000001 Rmu_sc0007801.1_g000002 Rmu_sc0007992.1_g000008 Rmu_sc0008698.1_g000003 Rmu_sc0010817.1_g000001 Rmu_sc0010817.1_g000021 Rmu_sc0011207.1_g000001 Rmu_sc0012533.1_g000007 Rmu_sc0012742.1_g000008 Rmu_sc0014075.1_g000001 Rmu_sc0017009.1_g000004 Rmu_sc0018430.1_g000002 Rmu_sc0020146.1_g000003 Rmu_sc0021186.1_g000001 Rmu_sc0023004.1_g000001 Rmu_sc0024933.1_g000001 Rmu_sc0026002.1_g000001 Rmu_sc0026989.1_g000005 Rmu_sc0028107.1_g000001 Rmu_sc0028108.1_g000001 Rmu_sc0032899.1_g000001 Rmu_sc0039686.1_g000001 Rmu_ssc0000134.1_g000054 Rmu_ssc0000213.1_g000089 Rmu_ssc0000295.1_g000043 Rmu_ssc0000295.1_g000045 Rmu_ssc0000309.1_g000024 Rmu_ssc0000309.1_g000025 Rmu_ssc0000438.1_g000003 Rmu_ssc0000438.1_g000017 Rmu_ssc0000438.1_g000018
rosa_roxburghii Rroxscaffold_1G00020710 Rroxscaffold_2G00077350 Rroxscaffold_2G00077360 Rroxscaffold_2G00137810 Rroxscaffold_2G00142810 Rroxscaffold_2G00143580 Rroxscaffold_2G00152890 Rroxscaffold_3G00248850 Rroxscaffold_4G00324400 Rroxscaffold_4G00329960 Rroxscaffold_4G00329970 Rroxscaffold_5G00339350 Rroxscaffold_5G00343640 Rroxscaffold_6G00393540 Rroxscaffold_6G00400820 Rroxscaffold_6G00408530 Rroxscaffold_6G00408540 Rroxscaffold_6G00414590 Rroxscaffold_6G00414600 Rroxscaffold_7G00180540 Rroxscaffold_7G00199530 Rroxscaffold_7G00203170 Rroxscaffold_7G00217520
rosa_samantha Rh3DG208400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 669
AccI GTMKAC 1 cut(s) 148
AccII CGCG 1 cut(s) 771
AciI CCGC 7 cut(s) 228, 293, 356, 363, 366, 606, 667
AclWI GGATC 1 cut(s) 795
AcoI YGGCCR 1 cut(s) 640
AcsI RAATTY 2 cut(s) 410, 575
AcyI GRCGYC 1 cut(s) 173
AfiI CCNNNNNNNGG 4 cut(s) 653, 724, 810, 811
AgsI TTSAA 2 cut(s) 409, 509
AjnI CCWGG 3 cut(s) 81, 175, 276
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
Alw26I GTCTC 2 cut(s) 37, 267
AlwI GGATC 1 cut(s) 795
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 5 cut(s) 85, 197, 221, 640, 715
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 2 cut(s) 410, 575
AspLEI GCGC 2 cut(s) 136, 693
AspS9I GGNCC 5 cut(s) 85, 86, 197, 716, 727
AsuC2I CCSGG 1 cut(s) 795
AsuHPI GGTGA 4 cut(s) 71, 77, 191, 809
AsuNHI GCTAGC 2 cut(s) 77, 358
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 1 cut(s) 727
BaeGI GKGCMC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 642
BanII GRGCYC 1 cut(s) 89
BbvCI CCTCAGC 1 cut(s) 548
BbvI GCAGC 1 cut(s) 145
BccI CCATC 5 cut(s) 43, 161, 162, 320, 473
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 3 cut(s) 83, 177, 278
BcnI CCSGG 1 cut(s) 795
BcoDI GTCTC 2 cut(s) 37, 267
BfaI CTAG 2 cut(s) 78, 359
BfmI CTRYAG 1 cut(s) 743
BglII AGATCT 1 cut(s) 747
BisI GCNGC 3 cut(s) 159, 363, 366
BlsI GCNGC 3 cut(s) 160, 364, 367
Bme1390I CCNGG 4 cut(s) 83, 177, 278, 795
Bme18I GGWCC 1 cut(s) 727
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 5 cut(s) 85, 86, 197, 716, 727
BmiI GGNNCC 4 cut(s) 87, 198, 718, 728
BmrFI CCNGG 4 cut(s) 83, 177, 278, 795
BmsI GCATC 2 cut(s) 106, 435
BmtI GCTAGC 2 cut(s) 81, 362
Bpu10I CCTNAGC 1 cut(s) 548
BpuEI CTTGAG 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 795
Bsa29I ATCGAT 1 cut(s) 315
BsaHI GRCGYC 1 cut(s) 173
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 82, 120, 237, 637
BsaWI WCCGGW 2 cut(s) 26, 537
Bsc4I CCNNNNNNNGG 4 cut(s) 653, 724, 810, 811
Bse1I ACTGG 3 cut(s) 224, 441, 718
Bse3DI GCAATG 1 cut(s) 104
BseBI CCWGG 3 cut(s) 83, 177, 278
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 4 cut(s) 82, 120, 237, 637
BseLI CCNNNNNNNGG 4 cut(s) 653, 724, 810, 811
BseMI GCAATG 1 cut(s) 104
BseMII CTCAG 1 cut(s) 562
BseNI ACTGG 3 cut(s) 224, 441, 718
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 1 cut(s) 89
BseXI GCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 336
Bsh1236I CGCG 1 cut(s) 771
BshFI GGCC 5 cut(s) 87, 199, 223, 642, 717
BshVI ATCGAT 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 3 cut(s) 27, 538, 795
BslI CCNNNNNNNGG 4 cut(s) 653, 724, 810, 811
BsmAI GTCTC 2 cut(s) 37, 267
BsmI GAATGC 1 cut(s) 504
BsnI GGCC 5 cut(s) 87, 199, 223, 642, 717
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 1 cut(s) 236
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 89
Bsp143I GATC 4 cut(s) 141, 316, 747, 787
Bsp19I CCATGG 1 cut(s) 120
Bsp68I TCGCGA 1 cut(s) 771
BspACI CCGC 7 cut(s) 228, 293, 356, 363, 366, 606, 667
BspANI GGCC 5 cut(s) 87, 199, 223, 642, 717
BspCNI CTCAG 1 cut(s) 561
BspDI ATCGAT 1 cut(s) 315
BspFNI CGCG 1 cut(s) 771
BspLI GGNNCC 4 cut(s) 87, 198, 718, 728
BspOI GCTAGC 2 cut(s) 81, 362
BspPI GGATC 1 cut(s) 795
BspQI GCTCTTC 1 cut(s) 168
BspTNI GGTCTC 1 cut(s) 267
BsrBI CCGCTC 1 cut(s) 669
BsrDI GCAATG 1 cut(s) 104
BsrI ACTGG 3 cut(s) 224, 441, 718
BssECI CCNNGG 4 cut(s) 82, 120, 237, 637
BssMI GATC 4 cut(s) 141, 316, 747, 787
BssNI GRCGYC 1 cut(s) 173
BssT1I CCWWGG 2 cut(s) 120, 637
Bst2UI CCWGG 3 cut(s) 83, 177, 278
Bst6I CTCTTC 2 cut(s) 27, 168
BstACI GRCGYC 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 79, 360
BstDEI CTNAG 1 cut(s) 548
BstDSI CCRYGG 1 cut(s) 120
BstFNI CGCG 1 cut(s) 771
BstHHI GCGC 2 cut(s) 136, 693
BstKTI GATC 4 cut(s) 144, 319, 750, 790
BstMAI GTCTC 2 cut(s) 37, 267
BstMBI GATC 4 cut(s) 141, 316, 747, 787
BstMWI GCNNNNNNNGC 3 cut(s) 359, 484, 768
BstNI CCWGG 3 cut(s) 83, 177, 278
BstSCI CCNGG 4 cut(s) 81, 175, 276, 793
BstSFI CTRYAG 1 cut(s) 743
BstSLI GKGCMC 1 cut(s) 89
BstUI CGCG 1 cut(s) 771
BstV1I GCAGC 1 cut(s) 145
BstX2I RGATCY 2 cut(s) 747, 787
BstYI RGATCY 2 cut(s) 747, 787
Bsu15I ATCGAT 1 cut(s) 315
BsuRI GGCC 5 cut(s) 87, 199, 223, 642, 717
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 1 cut(s) 120
BtgZI GCGATG 2 cut(s) 139, 786
BtsIMutI CAGTG 1 cut(s) 217
BtuMI TCGCGA 1 cut(s) 771
Cac8I GCNNGC 2 cut(s) 79, 360
CfoI GCGC 2 cut(s) 136, 693
Cfr13I GGNCC 5 cut(s) 85, 86, 197, 716, 727
ClaI ATCGAT 1 cut(s) 315
CseI GACGC 1 cut(s) 181
CviAII CATG 3 cut(s) 121, 488, 593
DdeI CTNAG 1 cut(s) 548
DpnI GATC 4 cut(s) 143, 318, 749, 789
DpnII GATC 4 cut(s) 141, 316, 747, 787
EaeI YGGCCR 1 cut(s) 640
Eam1104I CTCTTC 2 cut(s) 27, 168
EarI CTCTTC 2 cut(s) 27, 168
EciI GGCGGA 1 cut(s) 621
Eco130I CCWWGG 2 cut(s) 120, 637
Eco24I GRGCYC 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 727
Eco88I CYCGRG 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 86, 727
EcoRII CCWGG 3 cut(s) 81, 175, 276
EcoT14I CCWWGG 2 cut(s) 120, 637
EcoT38I GRGCYC 1 cut(s) 89
ErhI CCWWGG 2 cut(s) 120, 637
FaeI CATG 3 cut(s) 124, 491, 596
FaiI YATR 9 cut(s) 122, 132, 302, 483, 489, 561, 594, 650, 664
FatI CATG 3 cut(s) 120, 487, 592
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 159, 363, 366
FriOI GRGCYC 1 cut(s) 89
Fsp4HI GCNGC 3 cut(s) 159, 363, 366
FspBI CTAG 2 cut(s) 78, 359
GlaI GCGC 2 cut(s) 135, 692
GluI GCNGC 3 cut(s) 159, 363, 366
HaeIII GGCC 5 cut(s) 87, 199, 223, 642, 717
HapII CCGG 3 cut(s) 27, 538, 795
HgaI GACGC 1 cut(s) 181
HhaI GCGC 2 cut(s) 136, 693
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 3 cut(s) 124, 491, 596
Hin6I GCGC 2 cut(s) 134, 691
HinP1I GCGC 2 cut(s) 134, 691
HincII GTYRAC 2 cut(s) 149, 535
HindII GTYRAC 2 cut(s) 149, 535
HinfI GANTC 3 cut(s) 19, 609, 800
HpaII CCGG 3 cut(s) 27, 538, 795
HphI GGTGA 4 cut(s) 71, 77, 191, 809
Hpy166II GTNNAC 2 cut(s) 149, 535
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 3 cut(s) 257, 379, 770
Hpy8I GTNNAC 2 cut(s) 149, 535
Hpy99I CGWCG 1 cut(s) 771
HpyAV CCTTC 7 cut(s) 56, 99, 198, 341, 409, 730, 745
HpyCH4V TGCA 4 cut(s) 353, 487, 585, 659
HpyF10VI GCNNNNNNNGC 3 cut(s) 359, 484, 768
HpyF3I CTNAG 1 cut(s) 548
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 3 cut(s) 124, 491, 596
HspAI GCGC 2 cut(s) 134, 691
Kzo9I GATC 4 cut(s) 141, 316, 747, 787
LguI GCTCTTC 1 cut(s) 168
LmnI GCTCC 1 cut(s) 674
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 2 cut(s) 106, 435
MaeI CTAG 2 cut(s) 78, 359
MaeIII GTNAC 2 cut(s) 20, 215
MalI GATC 4 cut(s) 143, 318, 749, 789
MbiI CCGCTC 1 cut(s) 669
MboI GATC 4 cut(s) 141, 316, 747, 787
MboII GAAGA 2 cut(s) 44, 155
MfeI CAATTG 1 cut(s) 586
MflI RGATCY 2 cut(s) 747, 787
MhlI GDGCHC 1 cut(s) 89
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 5 cut(s) 410, 494, 509, 575, 586
MluNI TGGCCA 1 cut(s) 642
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 7 cut(s) 105, 232, 253, 283, 525, 557, 740
Mox20I TGGCCA 1 cut(s) 642
MscI TGGCCA 1 cut(s) 642
Msp20I TGGCCA 1 cut(s) 642
MspI CCGG 3 cut(s) 27, 538, 795
MspR9I CCNGG 4 cut(s) 83, 177, 278, 795
MunI CAATTG 1 cut(s) 586
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 3 cut(s) 83, 177, 278
MvnI CGCG 1 cut(s) 771
MwoI GCNNNNNNNGC 3 cut(s) 359, 484, 768
NciI CCSGG 1 cut(s) 795
NcoI CCATGG 1 cut(s) 120
NdeII GATC 4 cut(s) 141, 316, 747, 787
NheI GCTAGC 2 cut(s) 77, 358
NlaIII CATG 3 cut(s) 124, 491, 596
NlaIV GGNNCC 4 cut(s) 87, 198, 718, 728
NmuCI GTSAC 2 cut(s) 20, 215
NruI TCGCGA 1 cut(s) 771
PciSI GCTCTTC 1 cut(s) 168
PctI GAATGC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 609, 800
PkrI GCNGC 3 cut(s) 160, 364, 367
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PpuMI RGGWCCY 1 cut(s) 727
Psp5II RGGWCCY 1 cut(s) 727
Psp6I CCWGG 3 cut(s) 81, 175, 276
PspGI CCWGG 3 cut(s) 81, 175, 276
PspN4I GGNNCC 4 cut(s) 87, 198, 718, 728
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 5 cut(s) 85, 86, 197, 716, 727
PspPPI RGGWCCY 1 cut(s) 727
PsuI RGATCY 2 cut(s) 747, 787
RruI TCGCGA 1 cut(s) 771
SalI GTCGAC 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 168
SatI GCNGC 3 cut(s) 159, 363, 366
Sau3AI GATC 4 cut(s) 141, 316, 747, 787
Sau96I GGNCC 5 cut(s) 85, 86, 197, 716, 727
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 4 cut(s) 83, 177, 278, 795
SduI GDGCHC 1 cut(s) 89
SetI ASST 9 cut(s) 67, 79, 264, 279, 401, 534, 549, 649, 707
SfaNI GCATC 2 cut(s) 106, 435
SfcI CTRYAG 1 cut(s) 743
SinI GGWCC 1 cut(s) 727
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 5 cut(s) 410, 494, 509, 575, 586
SsiI CCGC 7 cut(s) 228, 293, 356, 363, 366, 606, 667
SspMI CTAG 2 cut(s) 78, 359
StyD4I CCNGG 4 cut(s) 81, 175, 276, 793
StyI CCWWGG 2 cut(s) 120, 637
TaqI TCGA 7 cut(s) 148, 166, 265, 315, 402, 468, 573
TasI AATT 5 cut(s) 410, 494, 509, 575, 586
TauI GCSGC 2 cut(s) 365, 368
TfiI GAWTC 2 cut(s) 609, 800
TscAI CASTG 1 cut(s) 224
TseFI GTSAC 2 cut(s) 20, 215
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 2 cut(s) 20, 215
TspDTI ATGAA 1 cut(s) 609
TspRI CASTG 1 cut(s) 224
VpaK11BI GGWCC 1 cut(s) 727
XapI RAATTY 2 cut(s) 410, 575
XmiI GTMKAC 1 cut(s) 148
XspI CTAG 2 cut(s) 78, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.