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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 112 |
| Acc36I | ACCTGC | 1 cut(s) | 112 |
| AccB1I | GGYRCC | 1 cut(s) | 124 |
| AccBSI | CCGCTC | 1 cut(s) | 320 |
| AccII | CGCG | 1 cut(s) | 51 |
| AciI | CCGC | 4 cut(s) | 97, 308, 315, 318 |
| AcsI | RAATTY | 1 cut(s) | 461 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 84, 408 |
| AjnI | CCWGG | 1 cut(s) | 127 |
| Alw26I | GTCTC | 1 cut(s) | 107 |
| AoxI | GGCC | 3 cut(s) | 75, 173, 231 |
| ApoI | RAATTY | 1 cut(s) | 461 |
| AspS9I | GGNCC | 3 cut(s) | 38, 149, 232 |
| AsuC2I | CCSGG | 1 cut(s) | 409 |
| AsuHPI | GGTGA | 4 cut(s) | 29, 143, 247, 397 |
| AsuNHI | GCTAGC | 1 cut(s) | 310 |
| AvaII | GGWCC | 2 cut(s) | 38, 149 |
| BanI | GGYRCC | 1 cut(s) | 124 |
| BbsI | GAAGAC | 1 cut(s) | 275 |
| BccI | CCATC | 5 cut(s) | 101, 188, 231, 272, 410 |
| BceAI | ACGGC | 1 cut(s) | 84 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 334, 368 |
| BciT130I | CCWGG | 1 cut(s) | 129 |
| BcnI | CCSGG | 1 cut(s) | 409 |
| BcoDI | GTCTC | 1 cut(s) | 107 |
| BfaI | CTAG | 3 cut(s) | 297, 311, 541 |
| BfuAI | ACCTGC | 1 cut(s) | 112 |
| BisI | GCNGC | 2 cut(s) | 315, 318 |
| BlsI | GCNGC | 2 cut(s) | 316, 319 |
| Bme1390I | CCNGG | 2 cut(s) | 129, 409 |
| Bme18I | GGWCC | 2 cut(s) | 38, 149 |
| BmgT120I | GGNCC | 3 cut(s) | 38, 149, 232 |
| BmiI | GGNNCC | 4 cut(s) | 39, 40, 126, 150 |
| BmrFI | CCNGG | 2 cut(s) | 129, 409 |
| BmsI | GCATC | 3 cut(s) | 58, 442, 505 |
| BmtI | GCTAGC | 1 cut(s) | 314 |
| BpiI | GAAGAC | 1 cut(s) | 275 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 367, 399 |
| BpmI | CTGGAG | 1 cut(s) | 325 |
| BpuMI | CCSGG | 1 cut(s) | 409 |
| Bsa29I | ATCGAT | 1 cut(s) | 267 |
| BsaJI | CCNNGG | 2 cut(s) | 152, 189 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 169, 199 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 84, 408 |
| BseBI | CCWGG | 1 cut(s) | 129 |
| BseCI | ATCGAT | 1 cut(s) | 267 |
| BseDI | CCNNGG | 2 cut(s) | 152, 189 |
| BseGI | GGATG | 3 cut(s) | 93, 247, 397 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 84, 408 |
| BsgI | GTGCAG | 2 cut(s) | 288, 520 |
| Bsh1236I | CGCG | 1 cut(s) | 51 |
| BshFI | GGCC | 3 cut(s) | 77, 175, 233 |
| BshNI | GGYRCC | 1 cut(s) | 124 |
| BshVI | ATCGAT | 1 cut(s) | 267 |
| BsiSI | CCGG | 1 cut(s) | 408 |
| BslFI | GGGAC | 2 cut(s) | 24, 162 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 84, 408 |
| BsmAI | GTCTC | 1 cut(s) | 107 |
| BsmBI | CGTCTC | 1 cut(s) | 107 |
| BsmFI | GGGAC | 2 cut(s) | 24, 162 |
| BsnI | GGCC | 3 cut(s) | 77, 175, 233 |
| Bsp143I | GATC | 1 cut(s) | 268 |
| Bsp68I | TCGCGA | 1 cut(s) | 51 |
| BspACI | CCGC | 4 cut(s) | 97, 308, 315, 318 |
| BspANI | GGCC | 3 cut(s) | 77, 175, 233 |
| BspDI | ATCGAT | 1 cut(s) | 267 |
| BspFNI | CGCG | 1 cut(s) | 51 |
| BspLI | GGNNCC | 4 cut(s) | 39, 40, 126, 150 |
| BspMI | ACCTGC | 1 cut(s) | 112 |
| BspOI | GCTAGC | 1 cut(s) | 314 |
| BspQI | GCTCTTC | 1 cut(s) | 120 |
| BspT107I | GGYRCC | 1 cut(s) | 124 |
| BsrBI | CCGCTC | 1 cut(s) | 320 |
| BssECI | CCNNGG | 2 cut(s) | 152, 189 |
| BssMI | GATC | 1 cut(s) | 268 |
| BssT1I | CCWWGG | 2 cut(s) | 152, 189 |
| Bst2UI | CCWGG | 1 cut(s) | 129 |
| Bst4CI | ACNGT | 1 cut(s) | 530 |
| Bst6I | CTCTTC | 1 cut(s) | 120 |
| BstC8I | GCNNGC | 1 cut(s) | 312 |
| BstF5I | GGATG | 3 cut(s) | 93, 247, 397 |
| BstFNI | CGCG | 1 cut(s) | 51 |
| BstKTI | GATC | 1 cut(s) | 271 |
| BstMAI | GTCTC | 1 cut(s) | 107 |
| BstMBI | GATC | 1 cut(s) | 268 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 311 |
| BstNI | CCWGG | 1 cut(s) | 129 |
| BstSCI | CCNGG | 2 cut(s) | 127, 407 |
| BstUI | CGCG | 1 cut(s) | 51 |
| BstV2I | GAAGAC | 1 cut(s) | 275 |
| Bsu15I | ATCGAT | 1 cut(s) | 267 |
| BsuRI | GGCC | 3 cut(s) | 77, 175, 233 |
| BsuTUI | ATCGAT | 1 cut(s) | 267 |
| BtgZI | GCGATG | 2 cut(s) | 166, 482 |
| BtsCI | GGATG | 3 cut(s) | 93, 247, 397 |
| BtuMI | TCGCGA | 1 cut(s) | 51 |
| BveI | ACCTGC | 1 cut(s) | 112 |
| Cac8I | GCNNGC | 1 cut(s) | 312 |
| Cfr13I | GGNCC | 3 cut(s) | 38, 149, 232 |
| ClaI | ATCGAT | 1 cut(s) | 267 |
| DpnI | GATC | 1 cut(s) | 270 |
| DpnII | GATC | 1 cut(s) | 268 |
| Eam1104I | CTCTTC | 1 cut(s) | 120 |
| EarI | CTCTTC | 1 cut(s) | 120 |
| EciI | GGCGGA | 1 cut(s) | 86 |
| Eco130I | CCWWGG | 2 cut(s) | 152, 189 |
| Eco47I | GGWCC | 2 cut(s) | 38, 149 |
| EcoO109I | RGGNCCY | 1 cut(s) | 38 |
| EcoRII | CCWGG | 1 cut(s) | 127 |
| EcoT14I | CCWWGG | 2 cut(s) | 152, 189 |
| ErhI | CCWWGG | 2 cut(s) | 152, 189 |
| Esp3I | CGTCTC | 1 cut(s) | 107 |
| FaiI | YATR | 9 cut(s) | 74, 84, 107, 274, 291, 380, 435, 441, 482 |
| FaqI | GGGAC | 2 cut(s) | 24, 162 |
| Fnu4HI | GCNGC | 2 cut(s) | 315, 318 |
| FokI | GGATG | 3 cut(s) | 80, 254, 404 |
| Fsp4HI | GCNGC | 2 cut(s) | 315, 318 |
| FspBI | CTAG | 3 cut(s) | 297, 311, 541 |
| GluI | GCNGC | 2 cut(s) | 315, 318 |
| GsuI | CTGGAG | 1 cut(s) | 325 |
| HaeIII | GGCC | 3 cut(s) | 77, 175, 233 |
| HapII | CCGG | 1 cut(s) | 408 |
| HinfI | GANTC | 1 cut(s) | 31 |
| HpaII | CCGG | 1 cut(s) | 408 |
| HphI | GGTGA | 4 cut(s) | 29, 143, 247, 397 |
| Hpy188III | TCNNGA | 3 cut(s) | 50, 209, 331 |
| HpyCH4III | ACNGT | 1 cut(s) | 530 |
| HpyCH4V | TGCA | 3 cut(s) | 305, 439, 537 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 311 |
| KflI | GGGWCCC | 1 cut(s) | 38 |
| Kzo9I | GATC | 1 cut(s) | 268 |
| LguI | GCTCTTC | 1 cut(s) | 120 |
| LmnI | GCTCC | 1 cut(s) | 190 |
| LweI | GCATC | 3 cut(s) | 58, 442, 505 |
| MaeI | CTAG | 3 cut(s) | 297, 311, 541 |
| MaeIII | GTNAC | 1 cut(s) | 530 |
| MalI | GATC | 1 cut(s) | 270 |
| MbiI | CCGCTC | 1 cut(s) | 320 |
| MboI | GATC | 1 cut(s) | 268 |
| MboII | GAAGA | 2 cut(s) | 107, 275 |
| MluCI | AATT | 1 cut(s) | 461 |
| MnlI | CCTC | 6 cut(s) | 8, 57, 103, 150, 205, 349 |
| MseI | TTAA | 1 cut(s) | 459 |
| MspI | CCGG | 1 cut(s) | 408 |
| MspR9I | CCNGG | 2 cut(s) | 129, 409 |
| MvaI | CCWGG | 1 cut(s) | 129 |
| MvnI | CGCG | 1 cut(s) | 51 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 311 |
| NciI | CCSGG | 1 cut(s) | 409 |
| NdeII | GATC | 1 cut(s) | 268 |
| NheI | GCTAGC | 1 cut(s) | 310 |
| NlaIV | GGNNCC | 4 cut(s) | 39, 40, 126, 150 |
| NruI | TCGCGA | 1 cut(s) | 51 |
| PaqCI | CACCTGC | 1 cut(s) | 112 |
| PciSI | GCTCTTC | 1 cut(s) | 120 |
| PfeI | GAWTC | 1 cut(s) | 31 |
| PkrI | GCNGC | 2 cut(s) | 316, 319 |
| PpuMI | RGGWCCY | 1 cut(s) | 38 |
| Psp5II | RGGWCCY | 1 cut(s) | 38 |
| Psp6I | CCWGG | 1 cut(s) | 127 |
| PspGI | CCWGG | 1 cut(s) | 127 |
| PspN4I | GGNNCC | 4 cut(s) | 39, 40, 126, 150 |
| PspPI | GGNCC | 3 cut(s) | 38, 149, 232 |
| PspPPI | RGGWCCY | 1 cut(s) | 38 |
| RruI | TCGCGA | 1 cut(s) | 51 |
| SapI | GCTCTTC | 1 cut(s) | 120 |
| SaqAI | TTAA | 1 cut(s) | 459 |
| SatI | GCNGC | 2 cut(s) | 315, 318 |
| Sau3AI | GATC | 1 cut(s) | 268 |
| Sau96I | GGNCC | 3 cut(s) | 38, 149, 232 |
| ScrFI | CCNGG | 2 cut(s) | 129, 409 |
| SetI | ASST | 6 cut(s) | 19, 49, 126, 216, 341, 542 |
| SfaNI | GCATC | 3 cut(s) | 58, 442, 505 |
| SinI | GGWCC | 2 cut(s) | 38, 149 |
| Sse9I | AATT | 1 cut(s) | 461 |
| SsiI | CCGC | 4 cut(s) | 97, 308, 315, 318 |
| SspMI | CTAG | 3 cut(s) | 297, 311, 541 |
| StyD4I | CCNGG | 2 cut(s) | 127, 407 |
| StyI | CCWWGG | 2 cut(s) | 152, 189 |
| TaaI | ACNGT | 1 cut(s) | 530 |
| TaqI | TCGA | 3 cut(s) | 217, 267, 354 |
| TasI | AATT | 1 cut(s) | 461 |
| TauI | GCSGC | 2 cut(s) | 317, 320 |
| TfiI | GAWTC | 1 cut(s) | 31 |
| Tru1I | TTAA | 1 cut(s) | 459 |
| Tru9I | TTAA | 1 cut(s) | 459 |
| VpaK11BI | GGWCC | 2 cut(s) | 38, 149 |
| XapI | RAATTY | 1 cut(s) | 461 |
| XspI | CTAG | 3 cut(s) | 297, 311, 541 |