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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 100 |
| AciI | CCGC | 2 cut(s) | 95, 98 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 188 |
| AoxI | GGCC | 1 cut(s) | 21 |
| AspS9I | GGNCC | 2 cut(s) | 4, 22 |
| AvaII | GGWCC | 1 cut(s) | 4 |
| BbsI | GAAGAC | 1 cut(s) | 55 |
| BccI | CCATC | 3 cut(s) | 21, 52, 190 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 114, 148 |
| BfaI | CTAG | 2 cut(s) | 77, 91 |
| BisI | GCNGC | 2 cut(s) | 95, 98 |
| BlsI | GCNGC | 2 cut(s) | 96, 99 |
| Bme18I | GGWCC | 1 cut(s) | 4 |
| BmgT120I | GGNCC | 2 cut(s) | 4, 22 |
| BpiI | GAAGAC | 1 cut(s) | 55 |
| BpmI | CTGGAG | 1 cut(s) | 105 |
| Bsa29I | ATCGAT | 1 cut(s) | 47 |
| BsaWI | WCCGGW | 1 cut(s) | 140 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 188 |
| BseCI | ATCGAT | 1 cut(s) | 47 |
| BseGI | GGATG | 2 cut(s) | 37, 177 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 188 |
| BshFI | GGCC | 1 cut(s) | 23 |
| BshVI | ATCGAT | 1 cut(s) | 47 |
| BsiSI | CCGG | 1 cut(s) | 141 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 188 |
| BsnI | GGCC | 1 cut(s) | 23 |
| Bsp143I | GATC | 1 cut(s) | 48 |
| BspACI | CCGC | 2 cut(s) | 95, 98 |
| BspANI | GGCC | 1 cut(s) | 23 |
| BspDI | ATCGAT | 1 cut(s) | 47 |
| BsrBI | CCGCTC | 1 cut(s) | 100 |
| BssMI | GATC | 1 cut(s) | 48 |
| BstF5I | GGATG | 2 cut(s) | 37, 177 |
| BstKTI | GATC | 1 cut(s) | 51 |
| BstMBI | GATC | 1 cut(s) | 48 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 91 |
| BstV2I | GAAGAC | 1 cut(s) | 55 |
| Bsu15I | ATCGAT | 1 cut(s) | 47 |
| BsuRI | GGCC | 1 cut(s) | 23 |
| BsuTUI | ATCGAT | 1 cut(s) | 47 |
| BtsCI | GGATG | 2 cut(s) | 37, 177 |
| Cfr13I | GGNCC | 2 cut(s) | 4, 22 |
| ClaI | ATCGAT | 1 cut(s) | 47 |
| CviAII | CATG | 1 cut(s) | 220 |
| CviJI | RGCY | 3 cut(s) | 23, 80, 94 |
| CviKI_1 | RGCY | 3 cut(s) | 23, 80, 94 |
| DpnI | GATC | 1 cut(s) | 50 |
| DpnII | GATC | 1 cut(s) | 48 |
| Eco47I | GGWCC | 1 cut(s) | 4 |
| FaeI | CATG | 1 cut(s) | 223 |
| FaiI | YATR | 5 cut(s) | 54, 71, 160, 215, 221 |
| FatI | CATG | 1 cut(s) | 219 |
| Fnu4HI | GCNGC | 2 cut(s) | 95, 98 |
| FokI | GGATG | 2 cut(s) | 44, 184 |
| Fsp4HI | GCNGC | 2 cut(s) | 95, 98 |
| FspBI | CTAG | 2 cut(s) | 77, 91 |
| GluI | GCNGC | 2 cut(s) | 95, 98 |
| GsuI | CTGGAG | 1 cut(s) | 105 |
| HaeIII | GGCC | 1 cut(s) | 23 |
| HapII | CCGG | 1 cut(s) | 141 |
| Hin1II | CATG | 1 cut(s) | 223 |
| HpaII | CCGG | 1 cut(s) | 141 |
| Hpy188I | TCNGA | 1 cut(s) | 8 |
| Hpy188III | TCNNGA | 1 cut(s) | 111 |
| HpyCH4V | TGCA | 2 cut(s) | 85, 219 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 91 |
| Hsp92II | CATG | 1 cut(s) | 223 |
| Kzo9I | GATC | 1 cut(s) | 48 |
| LpnPI | CCDG | 4 cut(s) | 96, 135, 154, 154 |
| MaeI | CTAG | 2 cut(s) | 77, 91 |
| MalI | GATC | 1 cut(s) | 50 |
| MbiI | CCGCTC | 1 cut(s) | 100 |
| MboI | GATC | 1 cut(s) | 48 |
| MboII | GAAGA | 1 cut(s) | 55 |
| MnlI | CCTC | 2 cut(s) | 98, 129 |
| MspI | CCGG | 1 cut(s) | 141 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 91 |
| NdeII | GATC | 1 cut(s) | 48 |
| NlaIII | CATG | 1 cut(s) | 223 |
| PkrI | GCNGC | 2 cut(s) | 96, 99 |
| PspPI | GGNCC | 2 cut(s) | 4, 22 |
| SatI | GCNGC | 2 cut(s) | 95, 98 |
| Sau3AI | GATC | 1 cut(s) | 48 |
| Sau96I | GGNCC | 2 cut(s) | 4, 22 |
| SetI | ASST | 3 cut(s) | 6, 90, 121 |
| SinI | GGWCC | 1 cut(s) | 4 |
| SsiI | CCGC | 2 cut(s) | 95, 98 |
| SspMI | CTAG | 2 cut(s) | 77, 91 |
| TaqI | TCGA | 2 cut(s) | 47, 134 |
| TauI | GCSGC | 2 cut(s) | 97, 100 |
| VpaK11BI | GGWCC | 1 cut(s) | 4 |
| XspI | CTAG | 2 cut(s) | 77, 91 |