Rmu_sc0002183.1_g000015

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002183.1
OG0000214
Physical Location & Seq
Forward (+)
67744 .. 68682
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002183.1_g000015.1.cds

Sequence Viewer

Length: 876 bp
atgacaacagagattctcgcggcactagaggtccaagaggtgaatgaggtgaggcctcttcctcttggtgaccacgtaccagatattgacccgacaactcgagaggtaagccattgtttggccaatactttccatttccctttagatccaaccccgctctctgatatttctgaacctgaccaggattctgtttgtaccgaggaggtggctgcaaacgctgctggtcttatcggcgggacagtcgccccagagatggaaaatgatactgacggtggtagtgcccctcaagtctcgcgaacaaatgagacaacctttctagattctcctgcggctgaagggacagaccaacctgttgaagatgaagaaactgaggaagctgtccacctttttgcattggtggttcatgatcctgtggcgcctccgccgcctcctcctcctaccagattcgagagattgatgagggtgttggaggtaactcctccttctgctgtggatgaggctaagatggcgcttcacgaacatctgggtcctcgggtattggagactgacattctctcgcgagtcttggaagctctaaggtatcttctccaggagaaggctttgactcagcggcaatttagtgctatcgactctctgctgaatgaacttggtaggggtcgccaacgtcaatcggcctcgaacgcccaagaccaagacgctcaagaggctttgaatagtgtctctcgagaaatggacatctcttgcgatattttaaatgagcgagccatcctcctgcgggaactacaaatccaaaggtccgacttcaaacttcagatcgaggacctccaagcccatttagcctttgttgagaatgcgattgttgccgaagaagcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

31.95

Weight (kDa)

4.31

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000214)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0079611
rosa_multiflora Rmu_co8150358.1_g000001 Rmu_co8306601.1_g000001 Rmu_sc0000032.1_g000018 Rmu_sc0000110.1_g000025 Rmu_sc0000110.1_g000026 Rmu_sc0000126.1_g000018 Rmu_sc0000147.1_g000058 Rmu_sc0000187.1_g000010 Rmu_sc0000240.1_g000061 Rmu_sc0000240.1_g000062 Rmu_sc0000258.1_g000018 Rmu_sc0000258.1_g000020 Rmu_sc0000276.1_g000075 Rmu_sc0000276.1_g000076 Rmu_sc0000356.1_g000007 Rmu_sc0000360.1_g000033 Rmu_sc0000368.1_g000014 Rmu_sc0000368.1_g000026 Rmu_sc0000389.1_g000011 Rmu_sc0000588.1_g000024 Rmu_sc0000595.1_g000060 Rmu_sc0000744.1_g000144 Rmu_sc0000744.1_g000145 Rmu_sc0000770.1_g000053 Rmu_sc0000795.1_g000096 Rmu_sc0000810.1_g000011 Rmu_sc0000935.1_g000066 Rmu_sc0000945.1_g000014 Rmu_sc0000962.1_g000011 Rmu_sc0000962.1_g000023 Rmu_sc0000962.1_g000024 Rmu_sc0001047.1_g000012 Rmu_sc0001094.1_g000015 Rmu_sc0001097.1_g000032 Rmu_sc0001254.1_g000006 Rmu_sc0001264.1_g000026 Rmu_sc0001322.1_g000013 Rmu_sc0001373.1_g000001 Rmu_sc0001424.1_g000002 Rmu_sc0001455.1_g000009 Rmu_sc0001461.1_g000008 Rmu_sc0001461.1_g000058 Rmu_sc0001563.1_g000014 Rmu_sc0001793.1_g000012 Rmu_sc0001793.1_g000013 Rmu_sc0001796.1_g000010 Rmu_sc0001817.1_g000004 Rmu_sc0002141.1_g000011 Rmu_sc0002141.1_g000012 Rmu_sc0002183.1_g000010 Rmu_sc0002183.1_g000015 Rmu_sc0002212.1_g000033 Rmu_sc0002212.1_g000034 Rmu_sc0002263.1_g000049 Rmu_sc0002263.1_g000050 Rmu_sc0002263.1_g000055 Rmu_sc0002263.1_g000056 Rmu_sc0002489.1_g000039 Rmu_sc0002654.1_g000003 Rmu_sc0002680.1_g000036 Rmu_sc0002745.1_g000013 Rmu_sc0002751.1_g000001 Rmu_sc0002769.1_g000013 Rmu_sc0002972.1_g000004 Rmu_sc0002985.1_g000011 Rmu_sc0003199.1_g000002 Rmu_sc0003207.1_g000020 Rmu_sc0003402.1_g000005 Rmu_sc0003500.1_g000009 Rmu_sc0003500.1_g000010 Rmu_sc0003600.1_g000051 Rmu_sc0003809.1_g000025 Rmu_sc0003859.1_g000029 Rmu_sc0003859.1_g000030 Rmu_sc0003994.1_g000025 Rmu_sc0004085.1_g000010 Rmu_sc0004085.1_g000015 Rmu_sc0004165.1_g000061 Rmu_sc0004525.1_g000006 Rmu_sc0004549.1_g000012 Rmu_sc0004598.1_g000035 Rmu_sc0004835.1_g000007 Rmu_sc0004881.1_g000008 Rmu_sc0005325.1_g000004 Rmu_sc0005325.1_g000006 Rmu_sc0005608.1_g000003 Rmu_sc0005747.1_g000026 Rmu_sc0006153.1_g000059 Rmu_sc0006273.1_g000010 Rmu_sc0006737.1_g000002 Rmu_sc0006737.1_g000003 Rmu_sc0006984.1_g000006 Rmu_sc0006984.1_g000007 Rmu_sc0007170.1_g000001 Rmu_sc0007801.1_g000002 Rmu_sc0007992.1_g000008 Rmu_sc0008698.1_g000003 Rmu_sc0010817.1_g000001 Rmu_sc0010817.1_g000021 Rmu_sc0011207.1_g000001 Rmu_sc0012533.1_g000007 Rmu_sc0012742.1_g000008 Rmu_sc0014075.1_g000001 Rmu_sc0017009.1_g000004 Rmu_sc0018430.1_g000002 Rmu_sc0020146.1_g000003 Rmu_sc0021186.1_g000001 Rmu_sc0023004.1_g000001 Rmu_sc0024933.1_g000001 Rmu_sc0026002.1_g000001 Rmu_sc0026989.1_g000005 Rmu_sc0028107.1_g000001 Rmu_sc0028108.1_g000001 Rmu_sc0032899.1_g000001 Rmu_sc0039686.1_g000001 Rmu_ssc0000134.1_g000054 Rmu_ssc0000213.1_g000089 Rmu_ssc0000295.1_g000043 Rmu_ssc0000295.1_g000045 Rmu_ssc0000309.1_g000024 Rmu_ssc0000309.1_g000025 Rmu_ssc0000438.1_g000003 Rmu_ssc0000438.1_g000017 Rmu_ssc0000438.1_g000018
rosa_roxburghii Rroxscaffold_1G00020710 Rroxscaffold_2G00077350 Rroxscaffold_2G00077360 Rroxscaffold_2G00137810 Rroxscaffold_2G00142810 Rroxscaffold_2G00143580 Rroxscaffold_2G00152890 Rroxscaffold_3G00248850 Rroxscaffold_4G00324400 Rroxscaffold_4G00329960 Rroxscaffold_4G00329970 Rroxscaffold_5G00339350 Rroxscaffold_5G00343640 Rroxscaffold_6G00393540 Rroxscaffold_6G00400820 Rroxscaffold_6G00408530 Rroxscaffold_6G00408540 Rroxscaffold_6G00414590 Rroxscaffold_6G00414600 Rroxscaffold_7G00180540 Rroxscaffold_7G00199530 Rroxscaffold_7G00203170 Rroxscaffold_7G00217520
rosa_samantha Rh3DG208400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 415
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 157
AccII CGCG 3 cut(s) 20, 295, 559
AciI CCGC 8 cut(s) 20, 155, 234, 329, 422, 425, 610, 775
AclWI GGATC 2 cut(s) 140, 401
AcoI YGGCCR 1 cut(s) 120
AcuI CTGAAG 2 cut(s) 354, 794
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 2 cut(s) 78, 196
AfiI CCNNNNNNNGG 4 cut(s) 118, 253, 595, 775
AgsI TTSAA 3 cut(s) 356, 712, 805
AjnI CCWGG 2 cut(s) 180, 588
AloI GAACNNNNNNTCC 2 cut(s) 771, 803
AluBI AGCT 3 cut(s) 377, 572, 872
AluI AGCT 3 cut(s) 377, 572, 872
Alw26I GTCTC 4 cut(s) 295, 299, 536, 724
AlwI GGATC 2 cut(s) 140, 401
Ama87I CYCGRG 3 cut(s) 99, 531, 723
AoxI GGCC 3 cut(s) 53, 120, 672
ApeKI GCWGC 2 cut(s) 209, 218
AspLEI GCGC 2 cut(s) 418, 511
AspS9I GGNCC 4 cut(s) 31, 527, 795, 820
AsuHPI GGTGA 3 cut(s) 52, 61, 80
AvaI CYCGRG 3 cut(s) 99, 531, 723
AvaII GGWCC 4 cut(s) 31, 527, 795, 820
BaeGI GKGCMC 1 cut(s) 283
BalI TGGCCA 1 cut(s) 122
BanI GGYRCC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 196, 205
BccI CCATC 3 cut(s) 247, 499, 773
BciT130I CCWGG 2 cut(s) 182, 590
BcoDI GTCTC 4 cut(s) 295, 299, 536, 724
BfaI CTAG 2 cut(s) 26, 317
BfoI RGCGCY 2 cut(s) 419, 512
BisI GCNGC 6 cut(s) 21, 210, 219, 330, 425, 611
BlsI GCNGC 6 cut(s) 22, 211, 220, 331, 426, 612
Bme1390I CCNGG 2 cut(s) 182, 590
Bme18I GGWCC 4 cut(s) 31, 527, 795, 820
BmeT110I CYCGRG 3 cut(s) 99, 531, 723
BmgT120I GGNCC 4 cut(s) 31, 527, 795, 820
BmiI GGNNCC 2 cut(s) 417, 528
BmrFI CCNGG 2 cut(s) 182, 590
BplI GAGNNNNNCTC 2 cut(s) 753, 785
BpmI CTGGAG 1 cut(s) 572
BpuEI CTTGAG 2 cut(s) 270, 684
BsaAI YACGTR 1 cut(s) 76
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 2 cut(s) 198, 530
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 253, 595, 775
BseBI CCWGG 2 cut(s) 182, 590
BseDI CCNNGG 2 cut(s) 198, 530
BseGI GGATG 2 cut(s) 499, 765
BseLI CCNNNNNNNGG 4 cut(s) 118, 253, 595, 775
BseMII CTCAG 2 cut(s) 360, 620
BseRI GAGGAG 4 cut(s) 215, 420, 423, 468
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 2 cut(s) 196, 205
Bsh1236I CGCG 3 cut(s) 20, 295, 559
BshFI GGCC 3 cut(s) 55, 122, 674
BshNI GGYRCC 1 cut(s) 415
BsiHKCI CYCGRG 3 cut(s) 99, 531, 723
BslFI GGGAC 2 cut(s) 250, 352
BslI CCNNNNNNNGG 4 cut(s) 118, 253, 595, 775
BsmAI GTCTC 4 cut(s) 295, 299, 536, 724
BsmFI GGGAC 2 cut(s) 250, 352
BsmI GAATGC 1 cut(s) 856
BsnI GGCC 3 cut(s) 55, 122, 674
BsoBI CYCGRG 3 cut(s) 99, 531, 723
Bsp1286I GDGCHC 1 cut(s) 283
Bsp143I GATC 3 cut(s) 145, 406, 813
Bsp68I TCGCGA 2 cut(s) 295, 559
BspACI CCGC 8 cut(s) 20, 155, 234, 329, 422, 425, 610, 775
BspANI GGCC 3 cut(s) 55, 122, 674
BspCNI CTCAG 2 cut(s) 361, 619
BspFNI CGCG 3 cut(s) 20, 295, 559
BspHI TCATGA 1 cut(s) 403
BspLI GGNNCC 2 cut(s) 417, 528
BspPI GGATC 2 cut(s) 140, 401
BspT107I GGYRCC 1 cut(s) 415
BsrBI CCGCTC 1 cut(s) 157
BssECI CCNNGG 2 cut(s) 198, 530
BssMI GATC 3 cut(s) 145, 406, 813
BssNI GRCGYC 1 cut(s) 416
Bst2UI CCWGG 2 cut(s) 182, 590
Bst4CI ACNGT 2 cut(s) 241, 272
Bst6I CTCTTC 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 1 cut(s) 762
BstDEI CTNAG 4 cut(s) 369, 501, 575, 606
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 2 cut(s) 499, 765
BstFNI CGCG 3 cut(s) 20, 295, 559
BstH2I RGCGCY 2 cut(s) 419, 512
BstHHI GCGC 2 cut(s) 418, 511
BstKTI GATC 3 cut(s) 148, 409, 816
BstMAI GTCTC 4 cut(s) 295, 299, 536, 724
BstMBI GATC 3 cut(s) 145, 406, 813
BstMWI GCNNNNNNNGC 9 cut(s) 215, 218, 424, 506, 680, 704, 836, 860, 869
BstNI CCWGG 2 cut(s) 182, 590
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 180, 588
BstSLI GKGCMC 1 cut(s) 283
BstUI CGCG 3 cut(s) 20, 295, 559
BstV1I GCAGC 2 cut(s) 196, 205
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BsuRI GGCC 3 cut(s) 55, 122, 674
BtsCI GGATG 2 cut(s) 499, 765
BtuMI TCGCGA 2 cut(s) 295, 559
Cac8I GCNNGC 1 cut(s) 762
CciI TCATGA 1 cut(s) 403
CfoI GCGC 2 cut(s) 418, 511
Cfr13I GGNCC 4 cut(s) 31, 527, 795, 820
CseI GACGC 1 cut(s) 704
Csp6I GTAC 2 cut(s) 77, 195
CviAII CATG 1 cut(s) 404
CviQI GTAC 2 cut(s) 77, 195
DdeI CTNAG 4 cut(s) 369, 501, 575, 606
DinI GGCGCC 1 cut(s) 417
DpnI GATC 3 cut(s) 147, 408, 815
DpnII GATC 3 cut(s) 145, 406, 813
DraI TTTAAA 1 cut(s) 753
EaeI YGGCCR 1 cut(s) 120
Eam1104I CTCTTC 1 cut(s) 63
EarI CTCTTC 1 cut(s) 63
EciI GGCGGA 1 cut(s) 411
Eco147I AGGCCT 1 cut(s) 55
Eco47I GGWCC 4 cut(s) 31, 527, 795, 820
Eco57I CTGAAG 2 cut(s) 354, 794
Eco88I CYCGRG 3 cut(s) 99, 531, 723
Eco91I GGTNACC 1 cut(s) 68
EcoO109I RGGNCCY 2 cut(s) 527, 820
EcoO65I GGTNACC 1 cut(s) 68
EcoRII CCWGG 2 cut(s) 180, 588
EgeI GGCGCC 1 cut(s) 417
EheI GGCGCC 1 cut(s) 417
FaeI CATG 1 cut(s) 407
FaiI YATR 1 cut(s) 405
FaqI GGGAC 2 cut(s) 250, 352
FatI CATG 1 cut(s) 403
FauI CCCGC 3 cut(s) 162, 227, 768
Fnu4HI GCNGC 6 cut(s) 21, 210, 219, 330, 425, 611
FokI GGATG 2 cut(s) 506, 752
Fsp4HI GCNGC 6 cut(s) 21, 210, 219, 330, 425, 611
FspBI CTAG 2 cut(s) 26, 317
GlaI GCGC 2 cut(s) 417, 510
GluI GCNGC 6 cut(s) 21, 210, 219, 330, 425, 611
GsuI CTGGAG 1 cut(s) 572
HaeII RGCGCY 2 cut(s) 419, 512
HaeIII GGCC 3 cut(s) 55, 122, 674
HgaI GACGC 1 cut(s) 704
HhaI GCGC 2 cut(s) 418, 511
Hin1I GRCGYC 1 cut(s) 416
Hin1II CATG 1 cut(s) 407
Hin6I GCGC 2 cut(s) 416, 509
HinP1I GCGC 2 cut(s) 416, 509
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 7 cut(s) 13, 185, 320, 444, 561, 604, 629
HphI GGTGA 3 cut(s) 52, 61, 80
Hpy166II GTNNAC 1 cut(s) 382
Hpy188I TCNGA 4 cut(s) 163, 172, 799, 813
Hpy8I GTNNAC 1 cut(s) 382
HpyAV CCTTC 3 cut(s) 329, 492, 589
HpyCH4III ACNGT 2 cut(s) 241, 272
HpyCH4IV ACGT 2 cut(s) 75, 664
HpyCH4V TGCA 2 cut(s) 212, 392
HpyF10VI GCNNNNNNNGC 9 cut(s) 215, 218, 424, 506, 680, 704, 836, 860, 869
HpyF3I CTNAG 4 cut(s) 369, 501, 575, 606
HpySE526I ACGT 2 cut(s) 75, 664
Hsp92I GRCGYC 1 cut(s) 416
Hsp92II CATG 1 cut(s) 407
HspAI GCGC 2 cut(s) 416, 509
KasI GGCGCC 1 cut(s) 415
Kzo9I GATC 3 cut(s) 145, 406, 813
Lsp1109I GCAGC 2 cut(s) 196, 205
MaeI CTAG 2 cut(s) 26, 317
MaeII ACGT 2 cut(s) 75, 664
MaeIII GTNAC 2 cut(s) 68, 472
MalI GATC 3 cut(s) 147, 408, 815
MbiI CCGCTC 1 cut(s) 157
MboI GATC 3 cut(s) 145, 406, 813
MboII GAAGA 4 cut(s) 50, 368, 374, 575
MflI RGATCY 1 cut(s) 145
MhlI GDGCHC 1 cut(s) 283
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 1 cut(s) 614
MluNI TGGCCA 1 cut(s) 122
Mly113I GGCGCC 1 cut(s) 416
MlyI GAGTC 3 cut(s) 570, 598, 623
MmeI TCCRAC 3 cut(s) 173, 447, 822
Mox20I TGGCCA 1 cut(s) 122
MscI TGGCCA 1 cut(s) 122
MseI TTAA 2 cut(s) 752, 874
Msp20I TGGCCA 1 cut(s) 122
MspA1I CMGCKG 1 cut(s) 610
MspR9I CCNGG 2 cut(s) 182, 590
Mva1269I GAATGC 1 cut(s) 856
MvaI CCWGG 2 cut(s) 182, 590
MvnI CGCG 3 cut(s) 20, 295, 559
MwoI GCNNNNNNNGC 9 cut(s) 215, 218, 424, 506, 680, 704, 836, 860, 869
NarI GGCGCC 1 cut(s) 416
NdeII GATC 3 cut(s) 145, 406, 813
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 2 cut(s) 417, 528
NmuCI GTSAC 1 cut(s) 68
NruI TCGCGA 2 cut(s) 295, 559
PaeR7I CTCGAG 2 cut(s) 99, 723
PagI TCATGA 1 cut(s) 403
PceI AGGCCT 1 cut(s) 55
PctI GAATGC 1 cut(s) 856
PfeI GAWTC 4 cut(s) 13, 185, 320, 444
PflMI CCANNNNNTGG 1 cut(s) 118
PfoI TCCNGGA 1 cut(s) 588
PkrI GCNGC 6 cut(s) 22, 211, 220, 331, 426, 612
PleI GAGTC 3 cut(s) 569, 598, 623
PluTI GGCGCC 1 cut(s) 419
PpsI GAGTC 3 cut(s) 569, 598, 623
Ppu21I YACGTR 1 cut(s) 76
PpuMI RGGWCCY 2 cut(s) 527, 820
Psp5II RGGWCCY 2 cut(s) 527, 820
Psp6I CCWGG 2 cut(s) 180, 588
PspEI GGTNACC 1 cut(s) 68
PspGI CCWGG 2 cut(s) 180, 588
PspN4I GGNNCC 2 cut(s) 417, 528
PspPI GGNCC 4 cut(s) 31, 527, 795, 820
PspPPI RGGWCCY 2 cut(s) 527, 820
PsuI RGATCY 1 cut(s) 145
RruI TCGCGA 2 cut(s) 295, 559
RsaI GTAC 2 cut(s) 78, 196
RsaNI GTAC 2 cut(s) 77, 195
SaqAI TTAA 2 cut(s) 752, 874
SatI GCNGC 6 cut(s) 21, 210, 219, 330, 425, 611
Sau3AI GATC 3 cut(s) 145, 406, 813
Sau96I GGNCC 4 cut(s) 31, 527, 795, 820
SchI GAGTC 3 cut(s) 570, 598, 623
ScrFI CCNGG 2 cut(s) 182, 590
SduI GDGCHC 1 cut(s) 283
SfoI GGCGCC 1 cut(s) 417
Sfr274I CTCGAG 2 cut(s) 99, 723
SinI GGWCC 4 cut(s) 31, 527, 795, 820
SlaI CTCGAG 2 cut(s) 99, 723
SmlI CTYRAG 4 cut(s) 99, 285, 699, 723
SmoI CTYRAG 4 cut(s) 99, 285, 699, 723
Sse9I AATT 1 cut(s) 614
SseBI AGGCCT 1 cut(s) 55
SsiI CCGC 8 cut(s) 20, 155, 234, 329, 422, 425, 610, 775
SspDI GGCGCC 1 cut(s) 415
SspMI CTAG 2 cut(s) 26, 317
StuI AGGCCT 1 cut(s) 55
StyD4I CCNGG 2 cut(s) 180, 588
TaaI ACNGT 2 cut(s) 241, 272
TaiI ACGT 2 cut(s) 78, 667
TaqI TCGA 6 cut(s) 100, 447, 627, 677, 724, 816
TasI AATT 1 cut(s) 614
TauI GCSGC 4 cut(s) 23, 332, 427, 613
TfiI GAWTC 4 cut(s) 13, 185, 320, 444
Tru1I TTAA 2 cut(s) 752, 874
Tru9I TTAA 2 cut(s) 752, 874
TseFI GTSAC 1 cut(s) 68
TseI GCWGC 2 cut(s) 209, 218
Tsp45I GTSAC 1 cut(s) 68
TspDTI ATGAA 3 cut(s) 375, 392, 657
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 4 cut(s) 31, 527, 795, 820
XbaI TCTAGA 1 cut(s) 316
XhoI CTCGAG 2 cut(s) 99, 723
XspI CTAG 2 cut(s) 26, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.