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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 238 |
| AatII | GACGTC | 2 cut(s) | 100, 2002 |
| Acc36I | ACCTGC | 1 cut(s) | 238 |
| Acc65I | GGTACC | 1 cut(s) | 913 |
| AccB1I | GGYRCC | 2 cut(s) | 250, 913 |
| AccI | GTMKAC | 2 cut(s) | 94, 980 |
| AclWI | GGATC | 2 cut(s) | 786, 1322 |
| AcsI | RAATTY | 1 cut(s) | 1838 |
| AcuI | CTGAAG | 3 cut(s) | 1704, 1881, 2129 |
| AcyI | GRCGYC | 2 cut(s) | 97, 1999 |
| AfaI | GTAC | 5 cut(s) | 689, 915, 1040, 1620, 1660 |
| AfeI | AGCGCT | 1 cut(s) | 1187 |
| AjnI | CCWGG | 6 cut(s) | 253, 798, 917, 973, 1156, 2019 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 2085, 2106, 2117, 2138 |
| AluBI | AGCT | 7 cut(s) | 656, 865, 1582, 1640, 1808, 1859, 1904 |
| AluI | AGCT | 7 cut(s) | 656, 865, 1582, 1640, 1808, 1859, 1904 |
| Alw21I | GWGCWC | 3 cut(s) | 658, 867, 1584 |
| Alw26I | GTCTC | 5 cut(s) | 1289, 1377, 1759, 1763, 2059 |
| AlwI | GGATC | 2 cut(s) | 786, 1322 |
| Ama87I | CYCGRG | 2 cut(s) | 314, 2058 |
| Aor51HI | AGCGCT | 1 cut(s) | 1187 |
| ApaI | GGGCCC | 1 cut(s) | 1793 |
| ApeKI | GCWGC | 2 cut(s) | 1202, 1856 |
| ApoI | RAATTY | 1 cut(s) | 1838 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 17, 1648 |
| Asp718I | GGTACC | 1 cut(s) | 913 |
| AspA2I | CCTAGG | 1 cut(s) | 1268 |
| AspLEI | GCGC | 1 cut(s) | 1188 |
| AsuHPI | GGTGA | 7 cut(s) | 149, 155, 269, 568, 1603, 1622, 1835 |
| AvaI | CYCGRG | 2 cut(s) | 314, 2058 |
| AvrII | CCTAGG | 1 cut(s) | 1268 |
| BaeGI | GKGCMC | 1 cut(s) | 1793 |
| BanI | GGYRCC | 2 cut(s) | 250, 913 |
| BanII | GRGCYC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| BauI | CACGAG | 1 cut(s) | 1262 |
| BbsI | GAAGAC | 1 cut(s) | 1305 |
| Bbv12I | GWGCWC | 3 cut(s) | 658, 867, 1584 |
| BbvCI | CCTCAGC | 1 cut(s) | 1335 |
| BbvI | GCAGC | 2 cut(s) | 1214, 1868 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 334, 368 |
| BciT130I | CCWGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| BciVI | GTATCC | 1 cut(s) | 1245 |
| BcoDI | GTCTC | 5 cut(s) | 1289, 1377, 1759, 1763, 2059 |
| BfaI | CTAG | 4 cut(s) | 729, 1269, 1568, 1712 |
| BfmI | CTRYAG | 2 cut(s) | 1200, 1854 |
| BfoI | RGCGCY | 1 cut(s) | 1189 |
| BfuAI | ACCTGC | 1 cut(s) | 238 |
| BfuI | GTATCC | 1 cut(s) | 1245 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 800 |
| BisI | GCNGC | 9 cut(s) | 57, 237, 583, 637, 1203, 1563, 1829, 1857, 2010 |
| BlnI | CCTAGG | 1 cut(s) | 1268 |
| BlsI | GCNGC | 9 cut(s) | 58, 238, 584, 638, 1204, 1564, 1830, 1858, 2011 |
| Bme1390I | CCNGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| BmeT110I | CYCGRG | 2 cut(s) | 314, 2058 |
| BmrFI | CCNGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| BmrI | ACTGGG | 1 cut(s) | 901 |
| BmsI | GCATC | 5 cut(s) | 14, 326, 505, 693, 1447 |
| BmuI | ACTGGG | 1 cut(s) | 901 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1775 |
| BpiI | GAAGAC | 1 cut(s) | 1305 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 367, 399, 1175, 1207 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1335 |
| BpuEI | CTTGAG | 2 cut(s) | 1202, 2019 |
| Bsa29I | ATCGAT | 2 cut(s) | 27, 1056 |
| BsaAI | YACGTR | 1 cut(s) | 1618 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1256, 1326 |
| BsaHI | GRCGYC | 2 cut(s) | 97, 1999 |
| BsaI | GGTCTC | 1 cut(s) | 1377 |
| BsaWI | WCCGGW | 3 cut(s) | 104, 360, 1632 |
| Bse1I | ACTGG | 3 cut(s) | 71, 632, 907 |
| Bse3DI | GCAATG | 1 cut(s) | 1341 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1256, 1326 |
| BseBI | CCWGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| BseCI | ATCGAT | 2 cut(s) | 27, 1056 |
| BseGI | GGATG | 3 cut(s) | 219, 226, 341 |
| BseJI | GATNNNNATC | 2 cut(s) | 1256, 1326 |
| BseMI | GCAATG | 1 cut(s) | 1341 |
| BseMII | CTCAG | 3 cut(s) | 1326, 1425, 1506 |
| BseNI | ACTGG | 3 cut(s) | 71, 632, 907 |
| BseRI | GAGGAG | 5 cut(s) | 125, 732, 1307, 1474, 1679 |
| BseSI | GKGCMC | 1 cut(s) | 1793 |
| BseXI | GCAGC | 2 cut(s) | 1214, 1868 |
| BseYI | CCCAGC | 2 cut(s) | 540, 694 |
| Bsh1285I | CGRYCG | 1 cut(s) | 181 |
| BshNI | GGYRCC | 2 cut(s) | 250, 913 |
| BshVI | ATCGAT | 2 cut(s) | 27, 1056 |
| BsiEI | CGRYCG | 1 cut(s) | 181 |
| BsiHKAI | GWGCWC | 3 cut(s) | 658, 867, 1584 |
| BsiHKCI | CYCGRG | 2 cut(s) | 314, 2058 |
| BsiSI | CCGG | 3 cut(s) | 105, 361, 1633 |
| BslFI | GGGAC | 4 cut(s) | 150, 288, 1118, 1714 |
| BsmAI | GTCTC | 5 cut(s) | 1289, 1377, 1759, 1763, 2059 |
| BsmFI | GGGAC | 4 cut(s) | 150, 288, 1118, 1714 |
| BsmI | GAATGC | 3 cut(s) | 59, 456, 1422 |
| Bso31I | GGTCTC | 1 cut(s) | 1377 |
| BsoBI | CYCGRG | 2 cut(s) | 314, 2058 |
| Bsp120I | GGGCCC | 1 cut(s) | 1789 |
| Bsp1286I | GDGCHC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| Bsp143I | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| Bsp68I | TCGCGA | 4 cut(s) | 177, 1759, 2028, 2079 |
| BspCNI | CTCAG | 3 cut(s) | 1327, 1426, 1507 |
| BspDI | ATCGAT | 2 cut(s) | 27, 1056 |
| BspHI | TCATGA | 1 cut(s) | 1318 |
| BspMAI | CTGCAG | 2 cut(s) | 1204, 1858 |
| BspMI | ACCTGC | 1 cut(s) | 238 |
| BspPI | GGATC | 2 cut(s) | 786, 1322 |
| BspQI | GCTCTTC | 1 cut(s) | 246 |
| BspT107I | GGYRCC | 2 cut(s) | 250, 913 |
| BspTNI | GGTCTC | 1 cut(s) | 1377 |
| BsrDI | GCAATG | 1 cut(s) | 1341 |
| BsrI | ACTGG | 3 cut(s) | 71, 632, 907 |
| BssMI | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| BssNI | GRCGYC | 2 cut(s) | 97, 1999 |
| BssSI | CACGAG | 1 cut(s) | 1262 |
| BssT1I | CCWWGG | 4 cut(s) | 278, 1022, 1247, 1268 |
| Bst2BI | CACGAG | 1 cut(s) | 1262 |
| Bst2UI | CCWGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| Bst4CI | ACNGT | 6 cut(s) | 143, 307, 1100, 1705, 1736, 1777 |
| Bst6I | CTCTTC | 4 cut(s) | 246, 1130, 1380, 1605 |
| BstACI | GRCGYC | 2 cut(s) | 97, 1999 |
| BstBAI | YACGTR | 1 cut(s) | 1618 |
| BstC8I | GCNNGC | 5 cut(s) | 889, 1357, 1993, 2026, 2108 |
| BstDEI | CTNAG | 3 cut(s) | 1335, 1434, 1515 |
| BstEII | GGTNACC | 1 cut(s) | 1610 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1112 |
| BstF5I | GGATG | 3 cut(s) | 219, 226, 341 |
| BstH2I | RGCGCY | 1 cut(s) | 1189 |
| BstHHI | GCGC | 1 cut(s) | 1188 |
| BstKTI | GATC | 9 cut(s) | 31, 181, 781, 850, 1137, 1330, 1470, 2099, 2151 |
| BstMAI | GTCTC | 5 cut(s) | 1289, 1377, 1759, 1763, 2059 |
| BstMBI | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| BstMCI | CGRYCG | 1 cut(s) | 181 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 800, 1352, 1506, 2030, 2039 |
| BstNI | CCWGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1775 |
| BstPI | GGTNACC | 1 cut(s) | 1610 |
| BstSCI | CCNGG | 6 cut(s) | 253, 798, 917, 973, 1156, 2019 |
| BstSFI | CTRYAG | 2 cut(s) | 1200, 1854 |
| BstSLI | GKGCMC | 1 cut(s) | 1793 |
| BstV1I | GCAGC | 2 cut(s) | 1214, 1868 |
| BstV2I | GAAGAC | 1 cut(s) | 1305 |
| BstX2I | RGATCY | 1 cut(s) | 1327 |
| BstYI | RGATCY | 1 cut(s) | 1327 |
| Bsu15I | ATCGAT | 2 cut(s) | 27, 1056 |
| BsuI | GTATCC | 1 cut(s) | 1245 |
| BsuTUI | ATCGAT | 2 cut(s) | 27, 1056 |
| BtsCI | GGATG | 3 cut(s) | 219, 226, 341 |
| BtsIMutI | CAGTG | 2 cut(s) | 843, 1680 |
| BtuMI | TCGCGA | 4 cut(s) | 177, 1759, 2028, 2079 |
| BveI | ACCTGC | 1 cut(s) | 238 |
| Cac8I | GCNNGC | 5 cut(s) | 889, 1357, 1993, 2026, 2108 |
| CciI | TCATGA | 1 cut(s) | 1318 |
| CfoI | GCGC | 1 cut(s) | 1188 |
| ClaI | ATCGAT | 2 cut(s) | 27, 1056 |
| CseI | GACGC | 2 cut(s) | 1232, 2039 |
| Csp6I | GTAC | 5 cut(s) | 688, 914, 1039, 1619, 1659 |
| CviAII | CATG | 6 cut(s) | 79, 440, 1319, 1400, 1493, 1820 |
| CviQI | GTAC | 5 cut(s) | 688, 914, 1039, 1619, 1659 |
| DdeI | CTNAG | 3 cut(s) | 1335, 1434, 1515 |
| DpnI | GATC | 9 cut(s) | 30, 180, 780, 849, 1136, 1329, 1469, 2098, 2150 |
| DpnII | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| DraI | TTTAAA | 1 cut(s) | 2088 |
| Eam1104I | CTCTTC | 4 cut(s) | 246, 1130, 1380, 1605 |
| EarI | CTCTTC | 4 cut(s) | 246, 1130, 1380, 1605 |
| EciI | GGCGGA | 3 cut(s) | 85, 212, 2093 |
| Ecl136II | GAGCTC | 3 cut(s) | 656, 865, 1582 |
| Eco130I | CCWWGG | 4 cut(s) | 278, 1022, 1247, 1268 |
| Eco147I | AGGCCT | 1 cut(s) | 1597 |
| Eco24I | GRGCYC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| Eco31I | GGTCTC | 1 cut(s) | 1377 |
| Eco32I | GATATC | 1 cut(s) | 1054 |
| Eco47III | AGCGCT | 1 cut(s) | 1187 |
| Eco53kI | GAGCTC | 3 cut(s) | 656, 865, 1582 |
| Eco57I | CTGAAG | 3 cut(s) | 1704, 1881, 2129 |
| Eco88I | CYCGRG | 2 cut(s) | 314, 2058 |
| Eco91I | GGTNACC | 1 cut(s) | 1610 |
| EcoICRI | GAGCTC | 3 cut(s) | 656, 865, 1582 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1112 |
| EcoO109I | RGGNCCY | 7 cut(s) | 164, 1105, 1448, 1688, 1789, 1790, 2155 |
| EcoO65I | GGTNACC | 1 cut(s) | 1610 |
| EcoRII | CCWGG | 6 cut(s) | 253, 798, 917, 973, 1156, 2019 |
| EcoRV | GATATC | 1 cut(s) | 1054 |
| EcoT14I | CCWWGG | 4 cut(s) | 278, 1022, 1247, 1268 |
| EcoT38I | GRGCYC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| ErhI | CCWWGG | 4 cut(s) | 278, 1022, 1247, 1268 |
| FaeI | CATG | 6 cut(s) | 82, 443, 1322, 1403, 1496, 1823 |
| FaqI | GGGAC | 4 cut(s) | 150, 288, 1118, 1714 |
| FatI | CATG | 6 cut(s) | 78, 439, 1318, 1399, 1492, 1819 |
| FauI | CCCGC | 3 cut(s) | 1691, 2000, 2103 |
| FblI | GTMKAC | 2 cut(s) | 94, 980 |
| Fnu4HI | GCNGC | 9 cut(s) | 57, 237, 583, 637, 1203, 1563, 1829, 1857, 2010 |
| FokI | GGATG | 3 cut(s) | 206, 213, 348 |
| FriOI | GRGCYC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| Fsp4HI | GCNGC | 9 cut(s) | 57, 237, 583, 637, 1203, 1563, 1829, 1857, 2010 |
| FspBI | CTAG | 4 cut(s) | 729, 1269, 1568, 1712 |
| GlaI | GCGC | 1 cut(s) | 1187 |
| GluI | GCNGC | 9 cut(s) | 57, 237, 583, 637, 1203, 1563, 1829, 1857, 2010 |
| GsaI | CCCAGC | 2 cut(s) | 544, 698 |
| HaeII | RGCGCY | 1 cut(s) | 1189 |
| HapII | CCGG | 3 cut(s) | 105, 361, 1633 |
| HgaI | GACGC | 2 cut(s) | 1232, 2039 |
| HhaI | GCGC | 1 cut(s) | 1188 |
| Hin1I | GRCGYC | 2 cut(s) | 97, 1999 |
| Hin1II | CATG | 6 cut(s) | 82, 443, 1322, 1403, 1496, 1823 |
| Hin6I | GCGC | 1 cut(s) | 1186 |
| HinP1I | GCGC | 1 cut(s) | 1186 |
| HpaII | CCGG | 3 cut(s) | 105, 361, 1633 |
| HphI | GGTGA | 7 cut(s) | 149, 155, 269, 568, 1603, 1622, 1835 |
| Hpy166II | GTNNAC | 5 cut(s) | 95, 981, 1002, 1096, 1429 |
| Hpy8I | GTNNAC | 5 cut(s) | 95, 981, 1002, 1096, 1429 |
| Hpy99I | CGWCG | 5 cut(s) | 582, 1385, 1781, 2001, 2033 |
| HpyAV | CCTTC | 5 cut(s) | 1159, 1239, 1679, 1856, 1924 |
| HpyCH4III | ACNGT | 6 cut(s) | 143, 307, 1100, 1705, 1736, 1777 |
| HpyCH4IV | ACGT | 4 cut(s) | 97, 642, 1617, 1999 |
| HpyCH4V | TGCA | 6 cut(s) | 439, 1202, 1359, 1444, 1856, 1919 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 800, 1352, 1506, 2030, 2039 |
| HpyF3I | CTNAG | 3 cut(s) | 1335, 1434, 1515 |
| HpySE526I | ACGT | 4 cut(s) | 97, 642, 1617, 1999 |
| Hsp92I | GRCGYC | 2 cut(s) | 97, 1999 |
| Hsp92II | CATG | 6 cut(s) | 82, 443, 1322, 1403, 1496, 1823 |
| HspAI | GCGC | 1 cut(s) | 1186 |
| KflI | GGGWCCC | 1 cut(s) | 164 |
| KpnI | GGTACC | 1 cut(s) | 917 |
| Kzo9I | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| LguI | GCTCTTC | 1 cut(s) | 246 |
| LmnI | GCTCC | 4 cut(s) | 661, 818, 1489, 1813 |
| Lsp1109I | GCAGC | 2 cut(s) | 1214, 1868 |
| LweI | GCATC | 5 cut(s) | 14, 326, 505, 693, 1447 |
| MaeI | CTAG | 4 cut(s) | 729, 1269, 1568, 1712 |
| MaeII | ACGT | 4 cut(s) | 97, 642, 1617, 1999 |
| MaeIII | GTNAC | 4 cut(s) | 137, 545, 857, 1610 |
| MalI | GATC | 9 cut(s) | 30, 180, 780, 849, 1136, 1329, 1469, 2098, 2150 |
| MboI | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| MboII | GAAGA | 9 cut(s) | 233, 1117, 1217, 1305, 1337, 1397, 1592, 1895, 1901 |
| MfeI | CAATTG | 1 cut(s) | 606 |
| MflI | RGATCY | 1 cut(s) | 1327 |
| MhlI | GDGCHC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| MluCI | AATT | 6 cut(s) | 461, 606, 893, 940, 1838, 1949 |
| MlyI | GAGTC | 7 cut(s) | 166, 1003, 1291, 1319, 1475, 1933, 1958 |
| MmeI | TCCRAC | 2 cut(s) | 1218, 2157 |
| MroXI | GAANNNNTTC | 2 cut(s) | 17, 1648 |
| MseI | TTAA | 1 cut(s) | 2087 |
| MslI | CAYNNNNRTG | 1 cut(s) | 554 |
| MspA1I | CMGCKG | 1 cut(s) | 1859 |
| MspI | CCGG | 3 cut(s) | 105, 361, 1633 |
| MspR9I | CCNGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| MunI | CAATTG | 1 cut(s) | 606 |
| Mva1269I | GAATGC | 3 cut(s) | 59, 456, 1422 |
| MvaI | CCWGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 800, 1352, 1506, 2030, 2039 |
| NdeII | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| NlaIII | CATG | 6 cut(s) | 82, 443, 1322, 1403, 1496, 1823 |
| NmeAIII | GCCGAG | 1 cut(s) | 1810 |
| NmuCI | GTSAC | 3 cut(s) | 137, 545, 1610 |
| NruI | TCGCGA | 4 cut(s) | 177, 1759, 2028, 2079 |
| PaeR7I | CTCGAG | 1 cut(s) | 2058 |
| PagI | TCATGA | 1 cut(s) | 1318 |
| PaqCI | CACCTGC | 1 cut(s) | 238 |
| PceI | AGGCCT | 1 cut(s) | 1597 |
| PciSI | GCTCTTC | 1 cut(s) | 246 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1240 |
| PctI | GAATGC | 3 cut(s) | 59, 456, 1422 |
| PdmI | GAANNNNTTC | 2 cut(s) | 17, 1648 |
| PfeI | GAWTC | 8 cut(s) | 611, 735, 932, 1015, 1177, 1649, 1802, 1847 |
| PkrI | GCNGC | 9 cut(s) | 58, 238, 584, 638, 1204, 1564, 1830, 1858, 2011 |
| Ple19I | CGATCG | 1 cut(s) | 181 |
| PleI | GAGTC | 7 cut(s) | 165, 1002, 1291, 1318, 1475, 1933, 1958 |
| PpsI | GAGTC | 7 cut(s) | 165, 1002, 1291, 1318, 1475, 1933, 1958 |
| Ppu21I | YACGTR | 1 cut(s) | 1618 |
| PpuMI | RGGWCCY | 5 cut(s) | 164, 1105, 1448, 1688, 2155 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1775 |
| Psp124BI | GAGCTC | 3 cut(s) | 658, 867, 1584 |
| Psp5II | RGGWCCY | 5 cut(s) | 164, 1105, 1448, 1688, 2155 |
| Psp6I | CCWGG | 6 cut(s) | 253, 798, 917, 973, 1156, 2019 |
| PspEI | GGTNACC | 1 cut(s) | 1610 |
| PspFI | CCCAGC | 2 cut(s) | 540, 694 |
| PspGI | CCWGG | 6 cut(s) | 253, 798, 917, 973, 1156, 2019 |
| PspOMI | GGGCCC | 1 cut(s) | 1789 |
| PspPPI | RGGWCCY | 5 cut(s) | 164, 1105, 1448, 1688, 2155 |
| PstI | CTGCAG | 2 cut(s) | 1204, 1858 |
| PsuI | RGATCY | 1 cut(s) | 1327 |
| PvuI | CGATCG | 1 cut(s) | 181 |
| PvuII | CAGCTG | 1 cut(s) | 1859 |
| RruI | TCGCGA | 4 cut(s) | 177, 1759, 2028, 2079 |
| RsaI | GTAC | 5 cut(s) | 689, 915, 1040, 1620, 1660 |
| RsaNI | GTAC | 5 cut(s) | 688, 914, 1039, 1619, 1659 |
| RseI | CAYNNNNRTG | 1 cut(s) | 554 |
| SacI | GAGCTC | 3 cut(s) | 658, 867, 1584 |
| SapI | GCTCTTC | 1 cut(s) | 246 |
| SaqAI | TTAA | 1 cut(s) | 2087 |
| SatI | GCNGC | 9 cut(s) | 57, 237, 583, 637, 1203, 1563, 1829, 1857, 2010 |
| Sau3AI | GATC | 9 cut(s) | 28, 178, 778, 847, 1134, 1327, 1467, 2096, 2148 |
| SchI | GAGTC | 7 cut(s) | 166, 1003, 1291, 1319, 1475, 1933, 1958 |
| ScrFI | CCNGG | 6 cut(s) | 255, 800, 919, 975, 1158, 2021 |
| SduI | GDGCHC | 5 cut(s) | 658, 796, 867, 1584, 1793 |
| SfaNI | GCATC | 5 cut(s) | 14, 326, 505, 693, 1447 |
| SfcI | CTRYAG | 2 cut(s) | 1200, 1854 |
| Sfr274I | CTCGAG | 1 cut(s) | 2058 |
| SlaI | CTCGAG | 1 cut(s) | 2058 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 554 |
| SmlI | CTYRAG | 3 cut(s) | 1181, 2034, 2058 |
| SmoI | CTYRAG | 3 cut(s) | 1181, 2034, 2058 |
| Sse9I | AATT | 6 cut(s) | 461, 606, 893, 940, 1838, 1949 |
| SseBI | AGGCCT | 1 cut(s) | 1597 |
| SspMI | CTAG | 4 cut(s) | 729, 1269, 1568, 1712 |
| SstI | GAGCTC | 3 cut(s) | 658, 867, 1584 |
| StuI | AGGCCT | 1 cut(s) | 1597 |
| StyD4I | CCNGG | 6 cut(s) | 253, 798, 917, 973, 1156, 2019 |
| StyI | CCWWGG | 4 cut(s) | 278, 1022, 1247, 1268 |
| TaaI | ACNGT | 6 cut(s) | 143, 307, 1100, 1705, 1736, 1777 |
| TaiI | ACGT | 4 cut(s) | 100, 645, 1620, 2002 |
| TasI | AATT | 6 cut(s) | 461, 606, 893, 940, 1838, 1949 |
| TatI | WGTACW | 1 cut(s) | 687 |
| TauI | GCSGC | 7 cut(s) | 59, 239, 585, 639, 1565, 1831, 2012 |
| TfiI | GAWTC | 8 cut(s) | 611, 735, 932, 1015, 1177, 1649, 1802, 1847 |
| Tru1I | TTAA | 1 cut(s) | 2087 |
| Tru9I | TTAA | 1 cut(s) | 2087 |
| TscAI | CASTG | 2 cut(s) | 850, 1687 |
| TseFI | GTSAC | 3 cut(s) | 137, 545, 1610 |
| TseI | GCWGC | 2 cut(s) | 1202, 1856 |
| Tsp45I | GTSAC | 3 cut(s) | 137, 545, 1610 |
| TspDTI | ATGAA | 7 cut(s) | 54, 1117, 1338, 1509, 1902, 1992, 2106 |
| TspGWI | ACGGA | 3 cut(s) | 566, 1081, 1369 |
| TspRI | CASTG | 2 cut(s) | 850, 1687 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1112 |
| XapI | RAATTY | 1 cut(s) | 1838 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 823 |
| XhoI | CTCGAG | 1 cut(s) | 2058 |
| XmaJI | CCTAGG | 1 cut(s) | 1268 |
| XmiI | GTMKAC | 2 cut(s) | 94, 980 |
| XmnI | GAANNNNTTC | 2 cut(s) | 17, 1648 |
| XspI | CTAG | 4 cut(s) | 729, 1269, 1568, 1712 |
| ZraI | GACGTC | 2 cut(s) | 98, 2000 |