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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 160 |
| Acc36I | ACCTGC | 1 cut(s) | 160 |
| AccB1I | GGYRCC | 1 cut(s) | 172 |
| AccBSI | CCGCTC | 1 cut(s) | 368 |
| AccII | CGCG | 2 cut(s) | 99, 573 |
| AciI | CCGC | 4 cut(s) | 283, 293, 356, 366 |
| AclWI | GGATC | 1 cut(s) | 110 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 132, 456 |
| AgsI | TTSAA | 1 cut(s) | 509 |
| AluBI | AGCT | 1 cut(s) | 592 |
| AluI | AGCT | 1 cut(s) | 592 |
| AlwI | GGATC | 1 cut(s) | 110 |
| Ama87I | CYCGRG | 1 cut(s) | 236 |
| AoxI | GGCC | 4 cut(s) | 123, 197, 221, 279 |
| ApeKI | GCWGC | 1 cut(s) | 549 |
| AspLEI | GCGC | 1 cut(s) | 573 |
| AspS9I | GGNCC | 4 cut(s) | 86, 197, 262, 280 |
| AsuHPI | GGTGA | 3 cut(s) | 77, 295, 439 |
| AsuNHI | GCTAGC | 1 cut(s) | 358 |
| AvaI | CYCGRG | 1 cut(s) | 236 |
| AvaII | GGWCC | 2 cut(s) | 86, 262 |
| BaeGI | GKGCMC | 1 cut(s) | 177 |
| BanI | GGYRCC | 1 cut(s) | 172 |
| BbvI | GCAGC | 1 cut(s) | 561 |
| BccI | CCATC | 6 cut(s) | 43, 149, 236, 320, 458, 494 |
| BceAI | ACGGC | 1 cut(s) | 132 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 382, 416 |
| BfaI | CTAG | 1 cut(s) | 359 |
| BfuAI | ACCTGC | 1 cut(s) | 160 |
| BisI | GCNGC | 1 cut(s) | 550 |
| BlsI | GCNGC | 1 cut(s) | 551 |
| Bme18I | GGWCC | 2 cut(s) | 86, 262 |
| BmeT110I | CYCGRG | 1 cut(s) | 236 |
| BmgT120I | GGNCC | 4 cut(s) | 86, 197, 262, 280 |
| BmiI | GGNNCC | 4 cut(s) | 87, 88, 174, 198 |
| BmsI | GCATC | 3 cut(s) | 2, 280, 553 |
| BmtI | GCTAGC | 1 cut(s) | 362 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 415, 447 |
| BpmI | CTGGAG | 1 cut(s) | 373 |
| BsaJI | CCNNGG | 2 cut(s) | 200, 237 |
| BsaWI | WCCGGW | 1 cut(s) | 408 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 217, 247 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 132, 456 |
| BseDI | CCNNGG | 2 cut(s) | 200, 237 |
| BseGI | GGATG | 2 cut(s) | 295, 445 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 132, 456 |
| BseRI | GAGGAG | 1 cut(s) | 47 |
| BseSI | GKGCMC | 1 cut(s) | 177 |
| BseXI | GCAGC | 1 cut(s) | 561 |
| BseYI | CCCAGC | 2 cut(s) | 285, 588 |
| BsgI | GTGCAG | 2 cut(s) | 336, 568 |
| Bsh1236I | CGCG | 2 cut(s) | 99, 573 |
| Bsh1285I | CGRYCG | 1 cut(s) | 103 |
| BshFI | GGCC | 4 cut(s) | 125, 199, 223, 281 |
| BshNI | GGYRCC | 1 cut(s) | 172 |
| BsiEI | CGRYCG | 1 cut(s) | 103 |
| BsiHKCI | CYCGRG | 1 cut(s) | 236 |
| BsiSI | CCGG | 1 cut(s) | 409 |
| BslFI | GGGAC | 2 cut(s) | 72, 135 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 132, 456 |
| BsmFI | GGGAC | 2 cut(s) | 72, 135 |
| BsmI | GAATGC | 1 cut(s) | 504 |
| BsnI | GGCC | 4 cut(s) | 125, 199, 223, 281 |
| BsoBI | CYCGRG | 1 cut(s) | 236 |
| Bsp1286I | GDGCHC | 1 cut(s) | 177 |
| Bsp143I | GATC | 2 cut(s) | 100, 115 |
| Bsp68I | TCGCGA | 1 cut(s) | 99 |
| BspACI | CCGC | 4 cut(s) | 283, 293, 356, 366 |
| BspANI | GGCC | 4 cut(s) | 125, 199, 223, 281 |
| BspFNI | CGCG | 2 cut(s) | 99, 573 |
| BspLI | GGNNCC | 4 cut(s) | 87, 88, 174, 198 |
| BspMI | ACCTGC | 1 cut(s) | 160 |
| BspOI | GCTAGC | 1 cut(s) | 362 |
| BspPI | GGATC | 1 cut(s) | 110 |
| BspQI | GCTCTTC | 1 cut(s) | 168 |
| BspT107I | GGYRCC | 1 cut(s) | 172 |
| BsrBI | CCGCTC | 1 cut(s) | 368 |
| BssECI | CCNNGG | 2 cut(s) | 200, 237 |
| BssMI | GATC | 2 cut(s) | 100, 115 |
| BssT1I | CCWWGG | 1 cut(s) | 200 |
| Bst6I | CTCTTC | 1 cut(s) | 168 |
| BstC8I | GCNNGC | 2 cut(s) | 283, 360 |
| BstF5I | GGATG | 2 cut(s) | 295, 445 |
| BstFNI | CGCG | 2 cut(s) | 99, 573 |
| BstHHI | GCGC | 1 cut(s) | 573 |
| BstKTI | GATC | 2 cut(s) | 103, 118 |
| BstMBI | GATC | 2 cut(s) | 100, 115 |
| BstMCI | CGRYCG | 1 cut(s) | 103 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 359 |
| BstSLI | GKGCMC | 1 cut(s) | 177 |
| BstUI | CGCG | 2 cut(s) | 99, 573 |
| BstV1I | GCAGC | 1 cut(s) | 561 |
| BsuRI | GGCC | 4 cut(s) | 125, 199, 223, 281 |
| BtgZI | GCGATG | 2 cut(s) | 214, 530 |
| BtsCI | GGATG | 2 cut(s) | 295, 445 |
| BtuMI | TCGCGA | 1 cut(s) | 99 |
| BveI | ACCTGC | 1 cut(s) | 160 |
| Cac8I | GCNNGC | 2 cut(s) | 283, 360 |
| CfoI | GCGC | 1 cut(s) | 573 |
| Cfr13I | GGNCC | 4 cut(s) | 86, 197, 262, 280 |
| CviAII | CATG | 3 cut(s) | 454, 488, 603 |
| DpnI | GATC | 2 cut(s) | 102, 117 |
| DpnII | GATC | 2 cut(s) | 100, 115 |
| Eam1104I | CTCTTC | 1 cut(s) | 168 |
| EarI | CTCTTC | 1 cut(s) | 168 |
| Eco130I | CCWWGG | 1 cut(s) | 200 |
| Eco47I | GGWCC | 2 cut(s) | 86, 262 |
| Eco88I | CYCGRG | 1 cut(s) | 236 |
| EcoO109I | RGGNCCY | 1 cut(s) | 86 |
| EcoT14I | CCWWGG | 1 cut(s) | 200 |
| ErhI | CCWWGG | 1 cut(s) | 200 |
| FaeI | CATG | 3 cut(s) | 457, 491, 606 |
| FaqI | GGGAC | 2 cut(s) | 72, 135 |
| FatI | CATG | 3 cut(s) | 453, 487, 602 |
| FauI | CCCGC | 1 cut(s) | 290 |
| Fnu4HI | GCNGC | 1 cut(s) | 550 |
| FokI | GGATG | 2 cut(s) | 302, 452 |
| Fsp4HI | GCNGC | 1 cut(s) | 550 |
| FspBI | CTAG | 1 cut(s) | 359 |
| GlaI | GCGC | 1 cut(s) | 572 |
| GluI | GCNGC | 1 cut(s) | 550 |
| GsaI | CCCAGC | 2 cut(s) | 289, 592 |
| GsuI | CTGGAG | 1 cut(s) | 373 |
| HaeIII | GGCC | 4 cut(s) | 125, 199, 223, 281 |
| HapII | CCGG | 1 cut(s) | 409 |
| HhaI | GCGC | 1 cut(s) | 573 |
| Hin1II | CATG | 3 cut(s) | 457, 491, 606 |
| Hin6I | GCGC | 1 cut(s) | 571 |
| HinP1I | GCGC | 1 cut(s) | 571 |
| HinfI | GANTC | 1 cut(s) | 79 |
| HpaII | CCGG | 1 cut(s) | 409 |
| HphI | GGTGA | 3 cut(s) | 77, 295, 439 |
| Hpy188I | TCNGA | 2 cut(s) | 24, 266 |
| Hpy188III | TCNNGA | 4 cut(s) | 98, 236, 257, 379 |
| HpyCH4V | TGCA | 4 cut(s) | 15, 353, 487, 585 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 359 |
| Hsp92II | CATG | 3 cut(s) | 457, 491, 606 |
| HspAI | GCGC | 1 cut(s) | 571 |
| KflI | GGGWCCC | 1 cut(s) | 86 |
| Kzo9I | GATC | 2 cut(s) | 100, 115 |
| LguI | GCTCTTC | 1 cut(s) | 168 |
| LmnI | GCTCC | 1 cut(s) | 238 |
| Lsp1109I | GCAGC | 1 cut(s) | 561 |
| LweI | GCATC | 3 cut(s) | 2, 280, 553 |
| MaeI | CTAG | 1 cut(s) | 359 |
| MalI | GATC | 2 cut(s) | 102, 117 |
| MbiI | CCGCTC | 1 cut(s) | 368 |
| MboI | GATC | 2 cut(s) | 100, 115 |
| MboII | GAAGA | 1 cut(s) | 155 |
| MhlI | GDGCHC | 1 cut(s) | 177 |
| MluCI | AATT | 1 cut(s) | 509 |
| MlyI | GAGTC | 1 cut(s) | 88 |
| MnlI | CCTC | 7 cut(s) | 25, 56, 105, 198, 232, 253, 397 |
| MslI | CAYNNNNRTG | 1 cut(s) | 452 |
| MspA1I | CMGCKG | 1 cut(s) | 285 |
| MspI | CCGG | 1 cut(s) | 409 |
| Mva1269I | GAATGC | 1 cut(s) | 504 |
| MvnI | CGCG | 2 cut(s) | 99, 573 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 359 |
| NdeII | GATC | 2 cut(s) | 100, 115 |
| NheI | GCTAGC | 1 cut(s) | 358 |
| NlaIII | CATG | 3 cut(s) | 457, 491, 606 |
| NlaIV | GGNNCC | 4 cut(s) | 87, 88, 174, 198 |
| NruI | TCGCGA | 1 cut(s) | 99 |
| PaqCI | CACCTGC | 1 cut(s) | 160 |
| PciSI | GCTCTTC | 1 cut(s) | 168 |
| PctI | GAATGC | 1 cut(s) | 504 |
| PkrI | GCNGC | 1 cut(s) | 551 |
| Ple19I | CGATCG | 1 cut(s) | 103 |
| PleI | GAGTC | 1 cut(s) | 87 |
| PpsI | GAGTC | 1 cut(s) | 87 |
| PpuMI | RGGWCCY | 1 cut(s) | 86 |
| Psp5II | RGGWCCY | 1 cut(s) | 86 |
| PspFI | CCCAGC | 2 cut(s) | 285, 588 |
| PspN4I | GGNNCC | 4 cut(s) | 87, 88, 174, 198 |
| PspPI | GGNCC | 4 cut(s) | 86, 197, 262, 280 |
| PspPPI | RGGWCCY | 1 cut(s) | 86 |
| PvuI | CGATCG | 1 cut(s) | 103 |
| RruI | TCGCGA | 1 cut(s) | 99 |
| RseI | CAYNNNNRTG | 1 cut(s) | 452 |
| SapI | GCTCTTC | 1 cut(s) | 168 |
| SatI | GCNGC | 1 cut(s) | 550 |
| Sau3AI | GATC | 2 cut(s) | 100, 115 |
| Sau96I | GGNCC | 4 cut(s) | 86, 197, 262, 280 |
| SchI | GAGTC | 1 cut(s) | 88 |
| SduI | GDGCHC | 1 cut(s) | 177 |
| SetI | ASST | 6 cut(s) | 67, 97, 174, 264, 389, 594 |
| SfaNI | GCATC | 3 cut(s) | 2, 280, 553 |
| SinI | GGWCC | 2 cut(s) | 86, 262 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 452 |
| Sse9I | AATT | 1 cut(s) | 509 |
| SsiI | CCGC | 4 cut(s) | 283, 293, 356, 366 |
| SspMI | CTAG | 1 cut(s) | 359 |
| StyI | CCWWGG | 1 cut(s) | 200 |
| TaqI | TCGA | 2 cut(s) | 10, 402 |
| TasI | AATT | 1 cut(s) | 509 |
| TseI | GCWGC | 1 cut(s) | 549 |
| VpaK11BI | GGWCC | 2 cut(s) | 86, 262 |
| XspI | CTAG | 1 cut(s) | 359 |