No description available
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 84 |
| AciI | CCGC | 3 cut(s) | 84, 139, 266 |
| AclWI | GGATC | 5 cut(s) | 34, 47, 95, 108, 288 |
| AcoI | YGGCCR | 1 cut(s) | 28 |
| AfaI | GTAC | 1 cut(s) | 191 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 123, 272 |
| AluBI | AGCT | 1 cut(s) | 158 |
| AluI | AGCT | 1 cut(s) | 158 |
| Alw21I | GWGCWC | 1 cut(s) | 160 |
| AlwI | GGATC | 5 cut(s) | 34, 47, 95, 108, 288 |
| AoxI | GGCC | 1 cut(s) | 28 |
| ApeKI | GCWGC | 1 cut(s) | 55 |
| AspA2I | CCTAGG | 1 cut(s) | 230 |
| AspLEI | GCGC | 1 cut(s) | 6 |
| AspS9I | GGNCC | 1 cut(s) | 211 |
| AsuHPI | GGTGA | 1 cut(s) | 98 |
| AvaII | GGWCC | 1 cut(s) | 211 |
| AvrII | CCTAGG | 1 cut(s) | 230 |
| BalI | TGGCCA | 1 cut(s) | 30 |
| BamHI | GGATCC | 2 cut(s) | 39, 100 |
| BanII | GRGCYC | 1 cut(s) | 160 |
| Bbv12I | GWGCWC | 1 cut(s) | 160 |
| BbvI | GCAGC | 1 cut(s) | 42 |
| BccI | CCATC | 3 cut(s) | 87, 251, 270 |
| BfaI | CTAG | 2 cut(s) | 231, 270 |
| BfmI | CTRYAG | 1 cut(s) | 56 |
| BisI | GCNGC | 2 cut(s) | 56, 139 |
| BlnI | CCTAGG | 1 cut(s) | 230 |
| BlsI | GCNGC | 2 cut(s) | 57, 140 |
| Bme18I | GGWCC | 1 cut(s) | 211 |
| BmgT120I | GGNCC | 1 cut(s) | 211 |
| BmiI | GGNNCC | 3 cut(s) | 41, 102, 213 |
| BmsI | GCATC | 1 cut(s) | 195 |
| BsaJI | CCNNGG | 1 cut(s) | 230 |
| BsaWI | WCCGGW | 1 cut(s) | 106 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 123, 272 |
| BseDI | CCNNGG | 1 cut(s) | 230 |
| BseGI | GGATG | 1 cut(s) | 281 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 123, 272 |
| BseXI | GCAGC | 1 cut(s) | 42 |
| Bsh1236I | CGCG | 1 cut(s) | 84 |
| Bsh1285I | CGRYCG | 1 cut(s) | 111 |
| BshFI | GGCC | 1 cut(s) | 30 |
| BsiEI | CGRYCG | 1 cut(s) | 111 |
| BsiHKAI | GWGCWC | 1 cut(s) | 160 |
| BsiSI | CCGG | 2 cut(s) | 107, 209 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 123, 272 |
| BsnI | GGCC | 1 cut(s) | 30 |
| Bsp1286I | GDGCHC | 1 cut(s) | 160 |
| Bsp143I | GATC | 3 cut(s) | 39, 100, 280 |
| BspACI | CCGC | 3 cut(s) | 84, 139, 266 |
| BspANI | GGCC | 1 cut(s) | 30 |
| BspFNI | CGCG | 1 cut(s) | 84 |
| BspLI | GGNNCC | 3 cut(s) | 41, 102, 213 |
| BspMAI | CTGCAG | 1 cut(s) | 60 |
| BspPI | GGATC | 5 cut(s) | 34, 47, 95, 108, 288 |
| BssECI | CCNNGG | 1 cut(s) | 230 |
| BssMI | GATC | 3 cut(s) | 39, 100, 280 |
| BssT1I | CCWWGG | 1 cut(s) | 230 |
| Bst6I | CTCTTC | 1 cut(s) | 195 |
| BstF5I | GGATG | 1 cut(s) | 281 |
| BstFNI | CGCG | 1 cut(s) | 84 |
| BstHHI | GCGC | 1 cut(s) | 6 |
| BstKTI | GATC | 3 cut(s) | 42, 103, 283 |
| BstMBI | GATC | 3 cut(s) | 39, 100, 280 |
| BstMCI | CGRYCG | 1 cut(s) | 111 |
| BstSFI | CTRYAG | 1 cut(s) | 56 |
| BstUI | CGCG | 1 cut(s) | 84 |
| BstV1I | GCAGC | 1 cut(s) | 42 |
| BstX2I | RGATCY | 2 cut(s) | 39, 100 |
| BstYI | RGATCY | 2 cut(s) | 39, 100 |
| BsuRI | GGCC | 1 cut(s) | 30 |
| BtgZI | GCGATG | 1 cut(s) | 65 |
| BtsCI | GGATG | 1 cut(s) | 281 |
| CfoI | GCGC | 1 cut(s) | 6 |
| Cfr13I | GGNCC | 1 cut(s) | 211 |
| CseI | GACGC | 1 cut(s) | 338 |
| Csp6I | GTAC | 1 cut(s) | 190 |
| CviJI | RGCY | 5 cut(s) | 30, 55, 133, 158, 261 |
| CviKI_1 | RGCY | 5 cut(s) | 30, 55, 133, 158, 261 |
| CviQI | GTAC | 1 cut(s) | 190 |
| DpnI | GATC | 3 cut(s) | 41, 102, 282 |
| DpnII | GATC | 3 cut(s) | 39, 100, 280 |
| EaeI | YGGCCR | 1 cut(s) | 28 |
| Eam1104I | CTCTTC | 1 cut(s) | 195 |
| EarI | CTCTTC | 1 cut(s) | 195 |
| EciI | GGCGGA | 1 cut(s) | 255 |
| Ecl136II | GAGCTC | 1 cut(s) | 158 |
| Eco130I | CCWWGG | 1 cut(s) | 230 |
| Eco24I | GRGCYC | 1 cut(s) | 160 |
| Eco47I | GGWCC | 1 cut(s) | 211 |
| Eco53kI | GAGCTC | 1 cut(s) | 158 |
| EcoICRI | GAGCTC | 1 cut(s) | 158 |
| EcoT14I | CCWWGG | 1 cut(s) | 230 |
| EcoT38I | GRGCYC | 1 cut(s) | 160 |
| ErhI | CCWWGG | 1 cut(s) | 230 |
| FaiI | YATR | 2 cut(s) | 285, 323 |
| Fnu4HI | GCNGC | 2 cut(s) | 56, 139 |
| FokI | GGATG | 1 cut(s) | 288 |
| FriOI | GRGCYC | 1 cut(s) | 160 |
| Fsp4HI | GCNGC | 2 cut(s) | 56, 139 |
| FspBI | CTAG | 2 cut(s) | 231, 270 |
| GlaI | GCGC | 1 cut(s) | 5 |
| GluI | GCNGC | 2 cut(s) | 56, 139 |
| HaeIII | GGCC | 1 cut(s) | 30 |
| HapII | CCGG | 2 cut(s) | 107, 209 |
| HgaI | GACGC | 1 cut(s) | 338 |
| HhaI | GCGC | 1 cut(s) | 6 |
| Hin6I | GCGC | 1 cut(s) | 4 |
| HinP1I | GCGC | 1 cut(s) | 4 |
| HinfI | GANTC | 3 cut(s) | 113, 237, 309 |
| HpaII | CCGG | 2 cut(s) | 107, 209 |
| HphI | GGTGA | 1 cut(s) | 98 |
| Hpy188I | TCNGA | 5 cut(s) | 172, 308, 314, 328, 357 |
| Hpy99I | CGWCG | 1 cut(s) | 335 |
| HpyAV | CCTTC | 2 cut(s) | 236, 254 |
| HpyCH4IV | ACGT | 1 cut(s) | 144 |
| HpyCH4V | TGCA | 1 cut(s) | 58 |
| HpySE526I | ACGT | 1 cut(s) | 144 |
| HspAI | GCGC | 1 cut(s) | 4 |
| Kzo9I | GATC | 3 cut(s) | 39, 100, 280 |
| LmnI | GCTCC | 1 cut(s) | 163 |
| LpnPI | CCDG | 3 cut(s) | 120, 182, 222 |
| Lsp1109I | GCAGC | 1 cut(s) | 42 |
| LweI | GCATC | 1 cut(s) | 195 |
| MaeI | CTAG | 2 cut(s) | 231, 270 |
| MaeII | ACGT | 1 cut(s) | 144 |
| MalI | GATC | 3 cut(s) | 41, 102, 282 |
| MboI | GATC | 3 cut(s) | 39, 100, 280 |
| MboII | GAAGA | 1 cut(s) | 212 |
| MflI | RGATCY | 2 cut(s) | 39, 100 |
| MhlI | GDGCHC | 1 cut(s) | 160 |
| MlsI | TGGCCA | 1 cut(s) | 30 |
| MluCI | AATT | 2 cut(s) | 127, 301 |
| MluNI | TGGCCA | 1 cut(s) | 30 |
| MnlI | CCTC | 3 cut(s) | 53, 81, 114 |
| Mox20I | TGGCCA | 1 cut(s) | 30 |
| MscI | TGGCCA | 1 cut(s) | 30 |
| MseI | TTAA | 2 cut(s) | 345, 361 |
| MslI | CAYNNNNRTG | 1 cut(s) | 84 |
| Msp20I | TGGCCA | 1 cut(s) | 30 |
| MspI | CCGG | 2 cut(s) | 107, 209 |
| MvnI | CGCG | 1 cut(s) | 84 |
| NdeII | GATC | 3 cut(s) | 39, 100, 280 |
| NlaIV | GGNNCC | 3 cut(s) | 41, 102, 213 |
| PfeI | GAWTC | 3 cut(s) | 113, 237, 309 |
| PkrI | GCNGC | 2 cut(s) | 57, 140 |
| Psp124BI | GAGCTC | 1 cut(s) | 160 |
| PspN4I | GGNNCC | 3 cut(s) | 41, 102, 213 |
| PspPI | GGNCC | 1 cut(s) | 211 |
| PstI | CTGCAG | 1 cut(s) | 60 |
| PsuI | RGATCY | 2 cut(s) | 39, 100 |
| RsaI | GTAC | 1 cut(s) | 191 |
| RsaNI | GTAC | 1 cut(s) | 190 |
| RseI | CAYNNNNRTG | 1 cut(s) | 84 |
| SacI | GAGCTC | 1 cut(s) | 160 |
| SaqAI | TTAA | 2 cut(s) | 345, 361 |
| SatI | GCNGC | 2 cut(s) | 56, 139 |
| Sau3AI | GATC | 3 cut(s) | 39, 100, 280 |
| Sau96I | GGNCC | 1 cut(s) | 211 |
| SduI | GDGCHC | 1 cut(s) | 160 |
| SetI | ASST | 3 cut(s) | 147, 152, 160 |
| SfaNI | GCATC | 1 cut(s) | 195 |
| SfcI | CTRYAG | 1 cut(s) | 56 |
| SinI | GGWCC | 1 cut(s) | 211 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 84 |
| Sse9I | AATT | 2 cut(s) | 127, 301 |
| SsiI | CCGC | 3 cut(s) | 84, 139, 266 |
| SspMI | CTAG | 2 cut(s) | 231, 270 |
| SstI | GAGCTC | 1 cut(s) | 160 |
| StyI | CCWWGG | 1 cut(s) | 230 |
| TaiI | ACGT | 1 cut(s) | 147 |
| TaqI | TCGA | 2 cut(s) | 111, 154 |
| TasI | AATT | 2 cut(s) | 127, 301 |
| TatI | WGTACW | 1 cut(s) | 189 |
| TauI | GCSGC | 1 cut(s) | 141 |
| TfiI | GAWTC | 3 cut(s) | 113, 237, 309 |
| Tru1I | TTAA | 2 cut(s) | 345, 361 |
| Tru9I | TTAA | 2 cut(s) | 345, 361 |
| TseI | GCWGC | 1 cut(s) | 55 |
| TspGWI | ACGGA | 1 cut(s) | 52 |
| VpaK11BI | GGWCC | 1 cut(s) | 211 |
| XmaJI | CCTAGG | 1 cut(s) | 230 |
| XspI | CTAG | 2 cut(s) | 231, 270 |