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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 68 |
| AccII | CGCG | 1 cut(s) | 467 |
| AciI | CCGC | 4 cut(s) | 9, 66, 467, 522 |
| AcoI | YGGCCR | 1 cut(s) | 391 |
| AcsI | RAATTY | 3 cut(s) | 325, 340, 383 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 156, 506 |
| AgsI | TTSAA | 1 cut(s) | 209 |
| AjnI | CCWGG | 2 cut(s) | 320, 378 |
| AluBI | AGCT | 1 cut(s) | 62 |
| AluI | AGCT | 1 cut(s) | 62 |
| AoxI | GGCC | 3 cut(s) | 375, 391, 422 |
| ApaI | GGGCCC | 1 cut(s) | 379 |
| ApeKI | GCWGC | 1 cut(s) | 62 |
| ApoI | RAATTY | 3 cut(s) | 325, 340, 383 |
| AspLEI | GCGC | 1 cut(s) | 273 |
| AspS9I | GGNCC | 2 cut(s) | 375, 376 |
| AsuHPI | GGTGA | 2 cut(s) | 11, 481 |
| AsuNHI | GCTAGC | 1 cut(s) | 58 |
| BaeGI | GKGCMC | 1 cut(s) | 379 |
| BalI | TGGCCA | 1 cut(s) | 393 |
| BanII | GRGCYC | 1 cut(s) | 379 |
| BbsI | GAAGAC | 1 cut(s) | 23 |
| BbvI | GCAGC | 1 cut(s) | 49 |
| BccI | CCATC | 2 cut(s) | 158, 194 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 82, 116 |
| BciT130I | CCWGG | 2 cut(s) | 322, 380 |
| BfaI | CTAG | 2 cut(s) | 45, 59 |
| BisI | GCNGC | 3 cut(s) | 63, 66, 522 |
| BlsI | GCNGC | 3 cut(s) | 64, 67, 523 |
| Bme1390I | CCNGG | 2 cut(s) | 322, 380 |
| BmgT120I | GGNCC | 2 cut(s) | 375, 376 |
| BmiI | GGNNCC | 2 cut(s) | 377, 485 |
| BmrFI | CCNGG | 2 cut(s) | 322, 380 |
| BmrI | ACTGGG | 1 cut(s) | 382 |
| BmtI | GCTAGC | 1 cut(s) | 62 |
| BmuI | ACTGGG | 1 cut(s) | 382 |
| BpiI | GAAGAC | 1 cut(s) | 23 |
| BpmI | CTGGAG | 2 cut(s) | 73, 473 |
| BsaJI | CCNNGG | 2 cut(s) | 378, 425 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 156, 506 |
| Bse1I | ACTGG | 4 cut(s) | 109, 377, 490, 517 |
| Bse3DI | GCAATG | 1 cut(s) | 531 |
| BseBI | CCWGG | 2 cut(s) | 322, 380 |
| BseDI | CCNNGG | 2 cut(s) | 378, 425 |
| BseGI | GGATG | 2 cut(s) | 11, 145 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 156, 506 |
| BseMI | GCAATG | 1 cut(s) | 531 |
| BseNI | ACTGG | 4 cut(s) | 109, 377, 490, 517 |
| BseSI | GKGCMC | 1 cut(s) | 379 |
| BseXI | GCAGC | 1 cut(s) | 49 |
| BseYI | CCCAGC | 1 cut(s) | 288 |
| BsgI | GTGCAG | 1 cut(s) | 36 |
| Bsh1236I | CGCG | 1 cut(s) | 467 |
| BshFI | GGCC | 3 cut(s) | 377, 393, 424 |
| BslFI | GGGAC | 1 cut(s) | 255 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 156, 506 |
| BsmFI | GGGAC | 1 cut(s) | 255 |
| BsmI | GAATGC | 1 cut(s) | 204 |
| BsnI | GGCC | 3 cut(s) | 377, 393, 424 |
| Bsp120I | GGGCCC | 1 cut(s) | 375 |
| Bsp1286I | GDGCHC | 1 cut(s) | 379 |
| BspACI | CCGC | 4 cut(s) | 9, 66, 467, 522 |
| BspANI | GGCC | 3 cut(s) | 377, 393, 424 |
| BspFNI | CGCG | 1 cut(s) | 467 |
| BspLI | GGNNCC | 2 cut(s) | 377, 485 |
| BspOI | GCTAGC | 1 cut(s) | 62 |
| BsrBI | CCGCTC | 1 cut(s) | 68 |
| BsrDI | GCAATG | 1 cut(s) | 531 |
| BsrI | ACTGG | 4 cut(s) | 109, 377, 490, 517 |
| BssECI | CCNNGG | 2 cut(s) | 378, 425 |
| BssT1I | CCWWGG | 1 cut(s) | 425 |
| Bst2UI | CCWGG | 2 cut(s) | 322, 380 |
| Bst4CI | ACNGT | 2 cut(s) | 29, 278 |
| Bst6I | CTCTTC | 1 cut(s) | 463 |
| BstC8I | GCNNGC | 1 cut(s) | 60 |
| BstF5I | GGATG | 2 cut(s) | 11, 145 |
| BstFNI | CGCG | 1 cut(s) | 467 |
| BstHHI | GCGC | 1 cut(s) | 273 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 59 |
| BstNI | CCWGG | 2 cut(s) | 322, 380 |
| BstSCI | CCNGG | 2 cut(s) | 320, 378 |
| BstSLI | GKGCMC | 1 cut(s) | 379 |
| BstUI | CGCG | 1 cut(s) | 467 |
| BstV1I | GCAGC | 1 cut(s) | 49 |
| BstV2I | GAAGAC | 1 cut(s) | 23 |
| BsuRI | GGCC | 3 cut(s) | 377, 393, 424 |
| BtsCI | GGATG | 2 cut(s) | 11, 145 |
| BtsI | GCAGTG | 2 cut(s) | 53, 394 |
| BtsIMutI | CAGTG | 2 cut(s) | 53, 394 |
| Cac8I | GCNNGC | 1 cut(s) | 60 |
| CfoI | GCGC | 1 cut(s) | 273 |
| Cfr13I | GGNCC | 2 cut(s) | 375, 376 |
| CviAII | CATG | 1 cut(s) | 188 |
| CviJI | RGCY | 8 cut(s) | 48, 62, 233, 333, 377, 393, 424, 516 |
| CviKI_1 | RGCY | 8 cut(s) | 48, 62, 233, 333, 377, 393, 424, 516 |
| EaeI | YGGCCR | 1 cut(s) | 391 |
| Eam1104I | CTCTTC | 1 cut(s) | 463 |
| EarI | CTCTTC | 1 cut(s) | 463 |
| Eco130I | CCWWGG | 1 cut(s) | 425 |
| Eco24I | GRGCYC | 1 cut(s) | 379 |
| EcoO109I | RGGNCCY | 1 cut(s) | 376 |
| EcoRI | GAATTC | 2 cut(s) | 325, 383 |
| EcoRII | CCWGG | 2 cut(s) | 320, 378 |
| EcoT14I | CCWWGG | 1 cut(s) | 425 |
| EcoT38I | GRGCYC | 1 cut(s) | 379 |
| ErhI | CCWWGG | 1 cut(s) | 425 |
| FaeI | CATG | 1 cut(s) | 191 |
| FaiI | YATR | 6 cut(s) | 39, 128, 183, 189, 230, 300 |
| FaqI | GGGAC | 1 cut(s) | 255 |
| FatI | CATG | 1 cut(s) | 187 |
| Fnu4HI | GCNGC | 3 cut(s) | 63, 66, 522 |
| FokI | GGATG | 2 cut(s) | 18, 152 |
| FriOI | GRGCYC | 1 cut(s) | 379 |
| Fsp4HI | GCNGC | 3 cut(s) | 63, 66, 522 |
| FspBI | CTAG | 2 cut(s) | 45, 59 |
| GlaI | GCGC | 1 cut(s) | 272 |
| GluI | GCNGC | 3 cut(s) | 63, 66, 522 |
| GsaI | CCCAGC | 1 cut(s) | 292 |
| GsuI | CTGGAG | 2 cut(s) | 73, 473 |
| HaeIII | GGCC | 3 cut(s) | 377, 393, 424 |
| HhaI | GCGC | 1 cut(s) | 273 |
| Hin1II | CATG | 1 cut(s) | 191 |
| Hin6I | GCGC | 1 cut(s) | 271 |
| HinP1I | GCGC | 1 cut(s) | 271 |
| HinfI | GANTC | 1 cut(s) | 496 |
| HphI | GGTGA | 2 cut(s) | 11, 481 |
| Hpy188III | TCNNGA | 1 cut(s) | 79 |
| Hpy99I | CGWCG | 1 cut(s) | 467 |
| HpyCH4III | ACNGT | 2 cut(s) | 29, 278 |
| HpyCH4V | TGCA | 4 cut(s) | 53, 187, 338, 353 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 59 |
| Hsp92II | CATG | 1 cut(s) | 191 |
| HspAI | GCGC | 1 cut(s) | 271 |
| Lsp1109I | GCAGC | 1 cut(s) | 49 |
| MaeI | CTAG | 2 cut(s) | 45, 59 |
| MaeIII | GTNAC | 2 cut(s) | 278, 293 |
| MbiI | CCGCTC | 1 cut(s) | 68 |
| MboII | GAAGA | 3 cut(s) | 23, 350, 450 |
| MhlI | GDGCHC | 1 cut(s) | 379 |
| MlsI | TGGCCA | 1 cut(s) | 393 |
| MluCI | AATT | 4 cut(s) | 209, 325, 340, 383 |
| MluNI | TGGCCA | 1 cut(s) | 393 |
| MmeI | TCCRAC | 1 cut(s) | 386 |
| MnlI | CCTC | 3 cut(s) | 97, 299, 497 |
| Mox20I | TGGCCA | 1 cut(s) | 393 |
| MscI | TGGCCA | 1 cut(s) | 393 |
| Msp20I | TGGCCA | 1 cut(s) | 393 |
| MspR9I | CCNGG | 2 cut(s) | 322, 380 |
| Mva1269I | GAATGC | 1 cut(s) | 204 |
| MvaI | CCWGG | 2 cut(s) | 322, 380 |
| MvnI | CGCG | 1 cut(s) | 467 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 59 |
| NheI | GCTAGC | 1 cut(s) | 58 |
| NlaIII | CATG | 1 cut(s) | 191 |
| NlaIV | GGNNCC | 2 cut(s) | 377, 485 |
| NmuCI | GTSAC | 1 cut(s) | 293 |
| PctI | GAATGC | 1 cut(s) | 204 |
| PfeI | GAWTC | 1 cut(s) | 496 |
| PfoI | TCCNGGA | 1 cut(s) | 320 |
| PkrI | GCNGC | 3 cut(s) | 64, 67, 523 |
| Psp6I | CCWGG | 2 cut(s) | 320, 378 |
| PspFI | CCCAGC | 1 cut(s) | 288 |
| PspGI | CCWGG | 2 cut(s) | 320, 378 |
| PspN4I | GGNNCC | 2 cut(s) | 377, 485 |
| PspOMI | GGGCCC | 1 cut(s) | 375 |
| PspPI | GGNCC | 2 cut(s) | 375, 376 |
| SatI | GCNGC | 3 cut(s) | 63, 66, 522 |
| Sau96I | GGNCC | 2 cut(s) | 375, 376 |
| ScrFI | CCNGG | 2 cut(s) | 322, 380 |
| SduI | GDGCHC | 1 cut(s) | 379 |
| SetI | ASST | 7 cut(s) | 24, 64, 89, 310, 371, 437, 489 |
| Sse9I | AATT | 4 cut(s) | 209, 325, 340, 383 |
| SsiI | CCGC | 4 cut(s) | 9, 66, 467, 522 |
| SspMI | CTAG | 2 cut(s) | 45, 59 |
| StyD4I | CCNGG | 2 cut(s) | 320, 378 |
| StyI | CCWWGG | 1 cut(s) | 425 |
| TaaI | ACNGT | 2 cut(s) | 29, 278 |
| TaqI | TCGA | 1 cut(s) | 102 |
| TasI | AATT | 4 cut(s) | 209, 325, 340, 383 |
| TauI | GCSGC | 2 cut(s) | 68, 524 |
| TfiI | GAWTC | 1 cut(s) | 496 |
| TscAI | CASTG | 2 cut(s) | 60, 401 |
| TseFI | GTSAC | 1 cut(s) | 293 |
| TseI | GCWGC | 1 cut(s) | 62 |
| Tsp45I | GTSAC | 1 cut(s) | 293 |
| TspGWI | ACGGA | 1 cut(s) | 451 |
| TspRI | CASTG | 2 cut(s) | 60, 401 |
| XapI | RAATTY | 3 cut(s) | 325, 340, 383 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 369 |
| XspI | CTAG | 2 cut(s) | 45, 59 |