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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 415 |
| AccB1I | GGYRCC | 1 cut(s) | 415 |
| AciI | CCGC | 2 cut(s) | 139, 460 |
| AclWI | GGATC | 3 cut(s) | 34, 47, 288 |
| AfaI | GTAC | 2 cut(s) | 191, 417 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 123, 276, 459, 477 |
| AgsI | TTSAA | 1 cut(s) | 331 |
| AjnI | CCWGG | 3 cut(s) | 300, 419, 475 |
| AluBI | AGCT | 2 cut(s) | 158, 367 |
| AluI | AGCT | 2 cut(s) | 158, 367 |
| Alw21I | GWGCWC | 2 cut(s) | 160, 369 |
| AlwI | GGATC | 3 cut(s) | 34, 47, 288 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 482 |
| AoxI | GGCC | 3 cut(s) | 28, 303, 422 |
| ApeKI | GCWGC | 1 cut(s) | 55 |
| Asp718I | GGTACC | 1 cut(s) | 415 |
| AspS9I | GGNCC | 2 cut(s) | 29, 406 |
| AsuHPI | GGTGA | 1 cut(s) | 98 |
| AvaII | GGWCC | 1 cut(s) | 406 |
| BamHI | GGATCC | 1 cut(s) | 39 |
| BanI | GGYRCC | 1 cut(s) | 415 |
| BanII | GRGCYC | 3 cut(s) | 160, 298, 369 |
| Bbv12I | GWGCWC | 2 cut(s) | 160, 369 |
| BbvI | GCAGC | 1 cut(s) | 42 |
| BccI | CCATC | 2 cut(s) | 251, 326 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 353, 387 |
| BciT130I | CCWGG | 3 cut(s) | 302, 421, 477 |
| BfaI | CTAG | 1 cut(s) | 231 |
| BfmI | CTRYAG | 1 cut(s) | 56 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 302 |
| BisI | GCNGC | 2 cut(s) | 56, 139 |
| BlsI | GCNGC | 2 cut(s) | 57, 140 |
| Bme1390I | CCNGG | 3 cut(s) | 302, 421, 477 |
| Bme18I | GGWCC | 1 cut(s) | 406 |
| BmgT120I | GGNCC | 2 cut(s) | 29, 406 |
| BmiI | GGNNCC | 3 cut(s) | 41, 102, 417 |
| BmrFI | CCNGG | 3 cut(s) | 302, 421, 477 |
| BmrI | ACTGGG | 1 cut(s) | 264 |
| BmsI | GCATC | 1 cut(s) | 195 |
| BmuI | ACTGGG | 1 cut(s) | 264 |
| BpmI | CTGGAG | 1 cut(s) | 90 |
| BsaJI | CCNNGG | 2 cut(s) | 419, 476 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 123, 276, 459, 477 |
| Bse1I | ACTGG | 3 cut(s) | 107, 134, 270 |
| BseBI | CCWGG | 3 cut(s) | 302, 421, 477 |
| BseDI | CCNNGG | 2 cut(s) | 419, 476 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 123, 276, 459, 477 |
| BseNI | ACTGG | 3 cut(s) | 107, 134, 270 |
| BseRI | GAGGAG | 1 cut(s) | 234 |
| BseXI | GCAGC | 1 cut(s) | 42 |
| BshFI | GGCC | 3 cut(s) | 30, 305, 424 |
| BshNI | GGYRCC | 1 cut(s) | 415 |
| BsiHKAI | GWGCWC | 2 cut(s) | 160, 369 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 123, 276, 459, 477 |
| BsnI | GGCC | 3 cut(s) | 30, 305, 424 |
| Bsp1286I | GDGCHC | 3 cut(s) | 160, 298, 369 |
| Bsp143I | GATC | 2 cut(s) | 39, 280 |
| BspACI | CCGC | 2 cut(s) | 139, 460 |
| BspANI | GGCC | 3 cut(s) | 30, 305, 424 |
| BspLI | GGNNCC | 3 cut(s) | 41, 102, 417 |
| BspMAI | CTGCAG | 1 cut(s) | 60 |
| BspPI | GGATC | 3 cut(s) | 34, 47, 288 |
| BspT107I | GGYRCC | 1 cut(s) | 415 |
| BsrI | ACTGG | 3 cut(s) | 107, 134, 270 |
| BssECI | CCNNGG | 2 cut(s) | 419, 476 |
| BssMI | GATC | 2 cut(s) | 39, 280 |
| Bst2UI | CCWGG | 3 cut(s) | 302, 421, 477 |
| BstC8I | GCNNGC | 1 cut(s) | 391 |
| BstDEI | CTNAG | 1 cut(s) | 525 |
| BstKTI | GATC | 2 cut(s) | 42, 283 |
| BstMBI | GATC | 2 cut(s) | 39, 280 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 302 |
| BstNI | CCWGG | 3 cut(s) | 302, 421, 477 |
| BstSCI | CCNGG | 3 cut(s) | 300, 419, 475 |
| BstSFI | CTRYAG | 1 cut(s) | 56 |
| BstV1I | GCAGC | 1 cut(s) | 42 |
| BstX2I | RGATCY | 1 cut(s) | 39 |
| BstYI | RGATCY | 1 cut(s) | 39 |
| BsuRI | GGCC | 3 cut(s) | 30, 305, 424 |
| BtgZI | GCGATG | 1 cut(s) | 296 |
| BtsI | GCAGTG | 1 cut(s) | 148 |
| BtsIMutI | CAGTG | 2 cut(s) | 148, 277 |
| Cac8I | GCNNGC | 1 cut(s) | 391 |
| CaiI | CAGNNNCTG | 1 cut(s) | 482 |
| Cfr13I | GGNCC | 2 cut(s) | 29, 406 |
| Csp6I | GTAC | 2 cut(s) | 190, 416 |
| CviQI | GTAC | 2 cut(s) | 190, 416 |
| DdeI | CTNAG | 1 cut(s) | 525 |
| DpnI | GATC | 2 cut(s) | 41, 282 |
| DpnII | GATC | 2 cut(s) | 39, 280 |
| EciI | GGCGGA | 1 cut(s) | 475 |
| Ecl136II | GAGCTC | 2 cut(s) | 158, 367 |
| Eco24I | GRGCYC | 3 cut(s) | 160, 298, 369 |
| Eco47I | GGWCC | 1 cut(s) | 406 |
| Eco53kI | GAGCTC | 2 cut(s) | 158, 367 |
| EcoICRI | GAGCTC | 2 cut(s) | 158, 367 |
| EcoRII | CCWGG | 3 cut(s) | 300, 419, 475 |
| EcoT38I | GRGCYC | 3 cut(s) | 160, 298, 369 |
| FaiI | YATR | 3 cut(s) | 285, 372, 495 |
| Fnu4HI | GCNGC | 2 cut(s) | 56, 139 |
| FriOI | GRGCYC | 3 cut(s) | 160, 298, 369 |
| Fsp4HI | GCNGC | 2 cut(s) | 56, 139 |
| FspBI | CTAG | 1 cut(s) | 231 |
| GluI | GCNGC | 2 cut(s) | 56, 139 |
| GsuI | CTGGAG | 1 cut(s) | 90 |
| HaeIII | GGCC | 3 cut(s) | 30, 305, 424 |
| HinfI | GANTC | 3 cut(s) | 113, 434, 517 |
| HphI | GGTGA | 1 cut(s) | 98 |
| Hpy166II | GTNNAC | 2 cut(s) | 275, 504 |
| Hpy188III | TCNNGA | 2 cut(s) | 37, 328 |
| Hpy8I | GTNNAC | 2 cut(s) | 275, 504 |
| Hpy99I | CGWCG | 1 cut(s) | 84 |
| HpyAV | CCTTC | 2 cut(s) | 19, 334 |
| HpyCH4V | TGCA | 2 cut(s) | 47, 58 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 302 |
| HpyF3I | CTNAG | 1 cut(s) | 525 |
| KpnI | GGTACC | 1 cut(s) | 419 |
| Kzo9I | GATC | 2 cut(s) | 39, 280 |
| LmnI | GCTCC | 2 cut(s) | 9, 163 |
| Lsp1109I | GCAGC | 1 cut(s) | 42 |
| LweI | GCATC | 1 cut(s) | 195 |
| MaeI | CTAG | 1 cut(s) | 231 |
| MaeIII | GTNAC | 1 cut(s) | 359 |
| MalI | GATC | 2 cut(s) | 41, 282 |
| MboI | GATC | 2 cut(s) | 39, 280 |
| MfeI | CAATTG | 1 cut(s) | 410 |
| MflI | RGATCY | 1 cut(s) | 39 |
| MhlI | GDGCHC | 3 cut(s) | 160, 298, 369 |
| MluCI | AATT | 3 cut(s) | 395, 410, 442 |
| MnlI | CCTC | 8 cut(s) | 81, 114, 252, 255, 308, 435, 463, 497 |
| MspR9I | CCNGG | 3 cut(s) | 302, 421, 477 |
| MunI | CAATTG | 1 cut(s) | 410 |
| MvaI | CCWGG | 3 cut(s) | 302, 421, 477 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 302 |
| NdeII | GATC | 2 cut(s) | 39, 280 |
| NlaIV | GGNNCC | 3 cut(s) | 41, 102, 417 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 79 |
| PfeI | GAWTC | 3 cut(s) | 113, 434, 517 |
| PkrI | GCNGC | 2 cut(s) | 57, 140 |
| Psp124BI | GAGCTC | 2 cut(s) | 160, 369 |
| Psp6I | CCWGG | 3 cut(s) | 300, 419, 475 |
| PspGI | CCWGG | 3 cut(s) | 300, 419, 475 |
| PspN4I | GGNNCC | 3 cut(s) | 41, 102, 417 |
| PspPI | GGNCC | 2 cut(s) | 29, 406 |
| PstI | CTGCAG | 1 cut(s) | 60 |
| PstNI | CAGNNNCTG | 1 cut(s) | 482 |
| PsuI | RGATCY | 1 cut(s) | 39 |
| RsaI | GTAC | 2 cut(s) | 191, 417 |
| RsaNI | GTAC | 2 cut(s) | 190, 416 |
| SacI | GAGCTC | 2 cut(s) | 160, 369 |
| SatI | GCNGC | 2 cut(s) | 56, 139 |
| Sau3AI | GATC | 2 cut(s) | 39, 280 |
| Sau96I | GGNCC | 2 cut(s) | 29, 406 |
| ScrFI | CCNGG | 3 cut(s) | 302, 421, 477 |
| SduI | GDGCHC | 3 cut(s) | 160, 298, 369 |
| SetI | ASST | 5 cut(s) | 106, 152, 160, 369, 455 |
| SfaNI | GCATC | 1 cut(s) | 195 |
| SfcI | CTRYAG | 1 cut(s) | 56 |
| SinI | GGWCC | 1 cut(s) | 406 |
| Sse9I | AATT | 3 cut(s) | 395, 410, 442 |
| SsiI | CCGC | 2 cut(s) | 139, 460 |
| SspI | AATATT | 1 cut(s) | 334 |
| SspMI | CTAG | 1 cut(s) | 231 |
| SstI | GAGCTC | 2 cut(s) | 160, 369 |
| StyD4I | CCNGG | 3 cut(s) | 300, 419, 475 |
| TaqI | TCGA | 3 cut(s) | 12, 111, 154 |
| TasI | AATT | 3 cut(s) | 395, 410, 442 |
| TatI | WGTACW | 1 cut(s) | 189 |
| TauI | GCSGC | 1 cut(s) | 141 |
| TfiI | GAWTC | 3 cut(s) | 113, 434, 517 |
| TscAI | CASTG | 2 cut(s) | 148, 277 |
| TseI | GCWGC | 1 cut(s) | 55 |
| TspDTI | ATGAA | 2 cut(s) | 299, 482 |
| TspGWI | ACGGA | 1 cut(s) | 68 |
| TspRI | CASTG | 2 cut(s) | 148, 277 |
| VpaK11BI | GGWCC | 1 cut(s) | 406 |
| XspI | CTAG | 1 cut(s) | 231 |