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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 427 |
| AccII | CGCG | 1 cut(s) | 244 |
| AciI | CCGC | 4 cut(s) | 242, 269, 275, 413 |
| AclWI | GGATC | 2 cut(s) | 54, 256 |
| AcuI | CTGAAG | 2 cut(s) | 43, 421 |
| AcvI | CACGTG | 1 cut(s) | 482 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 275, 485 |
| AgsI | TTSAA | 5 cut(s) | 9, 212, 305, 312, 500 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 250, 271, 282, 303 |
| AluBI | AGCT | 2 cut(s) | 456, 508 |
| AluI | AGCT | 2 cut(s) | 456, 508 |
| Alw26I | GTCTC | 1 cut(s) | 224 |
| AlwI | GGATC | 2 cut(s) | 54, 256 |
| Ama87I | CYCGRG | 3 cut(s) | 184, 190, 223 |
| AoxI | GGCC | 4 cut(s) | 410, 439, 448, 515 |
| AspLEI | GCGC | 1 cut(s) | 71 |
| AspS9I | GGNCC | 3 cut(s) | 180, 320, 410 |
| AsuHPI | GGTGA | 1 cut(s) | 69 |
| AvaI | CYCGRG | 3 cut(s) | 184, 190, 223 |
| AvaII | GGWCC | 2 cut(s) | 180, 320 |
| BanII | GRGCYC | 1 cut(s) | 191 |
| BbrPI | CACGTG | 1 cut(s) | 482 |
| BccI | CCATC | 2 cut(s) | 152, 273 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 441, 475 |
| BcoDI | GTCTC | 1 cut(s) | 224 |
| BfuAI | ACCTGC | 1 cut(s) | 427 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 447 |
| BisI | GCNGC | 1 cut(s) | 413 |
| BlsI | GCNGC | 1 cut(s) | 414 |
| Bme18I | GGWCC | 2 cut(s) | 180, 320 |
| BmeT110I | CYCGRG | 3 cut(s) | 184, 190, 223 |
| BmgT120I | GGNCC | 3 cut(s) | 180, 320, 410 |
| BmiI | GGNNCC | 1 cut(s) | 181 |
| BmsI | GCATC | 1 cut(s) | 185 |
| BpuEI | CTTGAG | 1 cut(s) | 184 |
| BsaAI | YACGTR | 1 cut(s) | 482 |
| BsaJI | CCNNGG | 1 cut(s) | 183 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 275, 485 |
| BseDI | CCNNGG | 1 cut(s) | 183 |
| BseGI | GGATG | 3 cut(s) | 152, 200, 265 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 275, 485 |
| BseMII | CTCAG | 1 cut(s) | 459 |
| BseRI | GAGGAG | 1 cut(s) | 76 |
| Bsh1236I | CGCG | 1 cut(s) | 244 |
| BshFI | GGCC | 4 cut(s) | 412, 441, 450, 517 |
| BsiHKCI | CYCGRG | 3 cut(s) | 184, 190, 223 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 275, 485 |
| BsmAI | GTCTC | 1 cut(s) | 224 |
| BsmI | GAATGC | 2 cut(s) | 71, 356 |
| BsnI | GGCC | 4 cut(s) | 412, 441, 450, 517 |
| BsoBI | CYCGRG | 3 cut(s) | 184, 190, 223 |
| Bsp1286I | GDGCHC | 1 cut(s) | 191 |
| Bsp143I | GATC | 3 cut(s) | 59, 261, 434 |
| BspACI | CCGC | 4 cut(s) | 242, 269, 275, 413 |
| BspANI | GGCC | 4 cut(s) | 412, 441, 450, 517 |
| BspCNI | CTCAG | 1 cut(s) | 458 |
| BspFNI | CGCG | 1 cut(s) | 244 |
| BspLI | GGNNCC | 1 cut(s) | 181 |
| BspMI | ACCTGC | 1 cut(s) | 427 |
| BspPI | GGATC | 2 cut(s) | 54, 256 |
| BssECI | CCNNGG | 1 cut(s) | 183 |
| BssMI | GATC | 3 cut(s) | 59, 261, 434 |
| Bst4CI | ACNGT | 1 cut(s) | 143 |
| Bst6I | CTCTTC | 1 cut(s) | 19 |
| BstBAI | YACGTR | 1 cut(s) | 482 |
| BstC8I | GCNNGC | 2 cut(s) | 273, 515 |
| BstDEI | CTNAG | 2 cut(s) | 154, 445 |
| BstF5I | GGATG | 3 cut(s) | 152, 200, 265 |
| BstFNI | CGCG | 1 cut(s) | 244 |
| BstHHI | GCGC | 1 cut(s) | 71 |
| BstKTI | GATC | 3 cut(s) | 62, 264, 437 |
| BstMAI | GTCTC | 1 cut(s) | 224 |
| BstMBI | GATC | 3 cut(s) | 59, 261, 434 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 195, 204, 336, 360, 447, 514 |
| BstUI | CGCG | 1 cut(s) | 244 |
| BsuRI | GGCC | 4 cut(s) | 412, 441, 450, 517 |
| BtsCI | GGATG | 3 cut(s) | 152, 200, 265 |
| BveI | ACCTGC | 1 cut(s) | 427 |
| Cac8I | GCNNGC | 2 cut(s) | 273, 515 |
| CfoI | GCGC | 1 cut(s) | 71 |
| Cfr13I | GGNCC | 3 cut(s) | 180, 320, 410 |
| DdeI | CTNAG | 2 cut(s) | 154, 445 |
| DpnI | GATC | 3 cut(s) | 61, 263, 436 |
| DpnII | GATC | 3 cut(s) | 59, 261, 434 |
| DraI | TTTAAA | 1 cut(s) | 253 |
| Eam1104I | CTCTTC | 1 cut(s) | 19 |
| EarI | CTCTTC | 1 cut(s) | 19 |
| EciI | GGCGGA | 1 cut(s) | 258 |
| Eco24I | GRGCYC | 1 cut(s) | 191 |
| Eco47I | GGWCC | 2 cut(s) | 180, 320 |
| Eco57I | CTGAAG | 2 cut(s) | 43, 421 |
| Eco72I | CACGTG | 1 cut(s) | 482 |
| Eco88I | CYCGRG | 3 cut(s) | 184, 190, 223 |
| EcoO109I | RGGNCCY | 2 cut(s) | 180, 320 |
| EcoT38I | GRGCYC | 1 cut(s) | 191 |
| FauI | CCCGC | 2 cut(s) | 249, 268 |
| Fnu4HI | GCNGC | 1 cut(s) | 413 |
| FokI | GGATG | 3 cut(s) | 159, 207, 252 |
| FriOI | GRGCYC | 1 cut(s) | 191 |
| Fsp4HI | GCNGC | 1 cut(s) | 413 |
| GlaI | GCGC | 1 cut(s) | 70 |
| GluI | GCNGC | 1 cut(s) | 413 |
| HaeIII | GGCC | 4 cut(s) | 412, 441, 450, 517 |
| HhaI | GCGC | 1 cut(s) | 71 |
| Hin6I | GCGC | 1 cut(s) | 69 |
| HinP1I | GCGC | 1 cut(s) | 69 |
| HinfI | GANTC | 1 cut(s) | 97 |
| HphI | GGTGA | 1 cut(s) | 69 |
| Hpy166II | GTNNAC | 1 cut(s) | 35 |
| Hpy188I | TCNGA | 2 cut(s) | 401, 432 |
| Hpy188III | TCNNGA | 6 cut(s) | 101, 168, 192, 201, 223, 225 |
| Hpy8I | GTNNAC | 1 cut(s) | 35 |
| HpyAV | CCTTC | 1 cut(s) | 145 |
| HpyCH4III | ACNGT | 1 cut(s) | 143 |
| HpyCH4IV | ACGT | 1 cut(s) | 481 |
| HpyCH4V | TGCA | 3 cut(s) | 45, 363, 513 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 195, 204, 336, 360, 447, 514 |
| HpyF3I | CTNAG | 2 cut(s) | 154, 445 |
| HpySE526I | ACGT | 1 cut(s) | 481 |
| HspAI | GCGC | 1 cut(s) | 69 |
| Kzo9I | GATC | 3 cut(s) | 59, 261, 434 |
| LpnPI | CCDG | 9 cut(s) | 76, 107, 162, 285, 423, 432, 432, 442, 499 |
| LweI | GCATC | 1 cut(s) | 185 |
| MaeII | ACGT | 1 cut(s) | 481 |
| MalI | GATC | 3 cut(s) | 61, 263, 436 |
| MboI | GATC | 3 cut(s) | 59, 261, 434 |
| MboII | GAAGA | 4 cut(s) | 21, 27, 36, 414 |
| MhlI | GDGCHC | 1 cut(s) | 191 |
| MluCI | AATT | 1 cut(s) | 501 |
| MmeI | TCCRAC | 1 cut(s) | 100 |
| MseI | TTAA | 1 cut(s) | 252 |
| Mva1269I | GAATGC | 2 cut(s) | 71, 356 |
| MvnI | CGCG | 1 cut(s) | 244 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 195, 204, 336, 360, 447, 514 |
| NdeII | GATC | 3 cut(s) | 59, 261, 434 |
| NlaIV | GGNNCC | 1 cut(s) | 181 |
| PaeR7I | CTCGAG | 1 cut(s) | 223 |
| PctI | GAATGC | 2 cut(s) | 71, 356 |
| PfeI | GAWTC | 1 cut(s) | 97 |
| PkrI | GCNGC | 1 cut(s) | 414 |
| PmaCI | CACGTG | 1 cut(s) | 482 |
| PmlI | CACGTG | 1 cut(s) | 482 |
| Ppu21I | YACGTR | 1 cut(s) | 482 |
| PpuMI | RGGWCCY | 2 cut(s) | 180, 320 |
| Psp5II | RGGWCCY | 2 cut(s) | 180, 320 |
| PspCI | CACGTG | 1 cut(s) | 482 |
| PspN4I | GGNNCC | 1 cut(s) | 181 |
| PspPI | GGNCC | 3 cut(s) | 180, 320, 410 |
| PspPPI | RGGWCCY | 2 cut(s) | 180, 320 |
| SaqAI | TTAA | 1 cut(s) | 252 |
| SatI | GCNGC | 1 cut(s) | 413 |
| Sau3AI | GATC | 3 cut(s) | 59, 261, 434 |
| Sau96I | GGNCC | 3 cut(s) | 180, 320, 410 |
| SduI | GDGCHC | 1 cut(s) | 191 |
| SetI | ASST | 8 cut(s) | 86, 127, 297, 325, 421, 458, 484, 510 |
| SfaNI | GCATC | 1 cut(s) | 185 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 447 |
| Sfr274I | CTCGAG | 1 cut(s) | 223 |
| SinI | GGWCC | 2 cut(s) | 180, 320 |
| SlaI | CTCGAG | 1 cut(s) | 223 |
| SmlI | CTYRAG | 2 cut(s) | 199, 223 |
| SmoI | CTYRAG | 2 cut(s) | 199, 223 |
| Sse9I | AATT | 1 cut(s) | 501 |
| SsiI | CCGC | 4 cut(s) | 242, 269, 275, 413 |
| TaaI | ACNGT | 1 cut(s) | 143 |
| TaiI | ACGT | 1 cut(s) | 484 |
| TaqI | TCGA | 5 cut(s) | 100, 224, 298, 316, 379 |
| TasI | AATT | 1 cut(s) | 501 |
| TauI | GCSGC | 1 cut(s) | 415 |
| TfiI | GAWTC | 1 cut(s) | 97 |
| Tru1I | TTAA | 1 cut(s) | 252 |
| Tru9I | TTAA | 1 cut(s) | 252 |
| TspDTI | ATGAA | 2 cut(s) | 28, 271 |
| VpaK11BI | GGWCC | 2 cut(s) | 180, 320 |
| XhoI | CTCGAG | 1 cut(s) | 223 |