Plant mobile domain
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 238 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1821 |
| AatII | GACGTC | 2 cut(s) | 100, 2785 |
| Acc36I | ACCTGC | 2 cut(s) | 238, 1356 |
| Acc65I | GGTACC | 1 cut(s) | 1153 |
| AccB1I | GGYRCC | 5 cut(s) | 250, 1153, 1924, 2333, 2533 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 2173 |
| AccBSI | CCGCTC | 1 cut(s) | 446 |
| AccI | GTMKAC | 3 cut(s) | 94, 1220, 1296 |
| AccIII | TCCGGA | 1 cut(s) | 1402 |
| AclWI | GGATC | 8 cut(s) | 772, 785, 1607, 1715, 1832, 2195, 2519, 2874 |
| AcoI | YGGCCR | 3 cut(s) | 654, 2175, 2790 |
| AcsI | RAATTY | 5 cut(s) | 587, 602, 646, 1783, 2239 |
| AcuI | CTGAAG | 3 cut(s) | 2472, 2912, 3039 |
| AcyI | GRCGYC | 3 cut(s) | 97, 2534, 2782 |
| AfaI | GTAC | 5 cut(s) | 929, 1155, 1280, 2133, 2251 |
| AfeI | AGCGCT | 1 cut(s) | 1427 |
| AgsI | TTSAA | 9 cut(s) | 64, 1648, 1790, 1904, 2090, 2393, 2474, 2830, 2923 |
| AjnI | CCWGG | 9 cut(s) | 253, 582, 641, 1157, 1213, 1396, 1486, 2706, 2802 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2990, 3022 |
| AloI | GAACNNNNNNTCC | 6 cut(s) | 1353, 1385, 2868, 2889, 2900, 2921 |
| Alw21I | GWGCWC | 4 cut(s) | 898, 1107, 1626, 1768 |
| Alw26I | GTCTC | 7 cut(s) | 1574, 1682, 2024, 2350, 2354, 2450, 2654 |
| AlwI | GGATC | 8 cut(s) | 772, 785, 1607, 1715, 1832, 2195, 2519, 2874 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1874 |
| Ama87I | CYCGRG | 4 cut(s) | 314, 2154, 2649, 2841 |
| Aor13HI | TCCGGA | 1 cut(s) | 1402 |
| Aor51HI | AGCGCT | 1 cut(s) | 1427 |
| ApaI | GGGCCC | 2 cut(s) | 642, 2384 |
| ApeKI | GCWGC | 6 cut(s) | 793, 2261, 2264, 2273, 2495, 3011 |
| ApoI | RAATTY | 5 cut(s) | 587, 602, 646, 1783, 2239 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 1349, 1353, 1381, 1385 |
| Asp718I | GGTACC | 1 cut(s) | 1153 |
| AspA2I | CCTAGG | 1 cut(s) | 1508 |
| AspLEI | GCGC | 4 cut(s) | 744, 1055, 1428, 2536 |
| AsuC2I | CCSGG | 1 cut(s) | 1528 |
| AsuHPI | GGTGA | 6 cut(s) | 269, 373, 2107, 2135, 2290, 2426 |
| AsuNHI | GCTAGC | 1 cut(s) | 436 |
| AvaI | CYCGRG | 4 cut(s) | 314, 2154, 2649, 2841 |
| AvrII | CCTAGG | 1 cut(s) | 1508 |
| AxyI | CCTNAGG | 1 cut(s) | 2445 |
| BaeGI | GKGCMC | 3 cut(s) | 642, 2338, 2384 |
| BalI | TGGCCA | 2 cut(s) | 656, 2177 |
| BamHI | GGATCC | 1 cut(s) | 777 |
| BanI | GGYRCC | 5 cut(s) | 250, 1153, 1924, 2333, 2533 |
| BanII | GRGCYC | 7 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2384 |
| BbsI | GAAGAC | 3 cut(s) | 401, 1590, 1698 |
| Bbv12I | GWGCWC | 4 cut(s) | 898, 1107, 1626, 1768 |
| BbvCI | CCTCAGC | 3 cut(s) | 1728, 1867, 2376 |
| BbvI | GCAGC | 6 cut(s) | 780, 2251, 2260, 2273, 2482, 2998 |
| BceAI | ACGGC | 2 cut(s) | 210, 719 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 460, 494, 1289, 1323 |
| BciT130I | CCWGG | 9 cut(s) | 255, 584, 643, 1159, 1215, 1398, 1488, 2708, 2804 |
| BciVI | GTATCC | 1 cut(s) | 1485 |
| BcnI | CCSGG | 1 cut(s) | 1528 |
| BcoDI | GTCTC | 7 cut(s) | 1574, 1682, 2024, 2350, 2354, 2450, 2654 |
| BfaI | CTAG | 6 cut(s) | 147, 423, 437, 969, 1509, 2081 |
| BfmI | CTRYAG | 3 cut(s) | 794, 1440, 1875 |
| BfoI | RGCGCY | 2 cut(s) | 1429, 2537 |
| BfuAI | ACCTGC | 2 cut(s) | 238, 1356 |
| BfuI | GTATCC | 1 cut(s) | 1485 |
| BlnI | CCTAGG | 1 cut(s) | 1508 |
| BlpI | GCTNAGC | 1 cut(s) | 1620 |
| BmeT110I | CYCGRG | 4 cut(s) | 314, 2154, 2649, 2841 |
| BmrI | ACTGGG | 3 cut(s) | 645, 1002, 1141 |
| BmsI | GCATC | 3 cut(s) | 14, 1916, 1960 |
| BmtI | GCTAGC | 1 cut(s) | 440 |
| BmuI | ACTGGG | 3 cut(s) | 645, 1002, 1141 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2366 |
| BpiI | GAAGAC | 3 cut(s) | 401, 1590, 1698 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 493, 525, 1023, 1055, 1415, 1447 |
| BpmI | CTGGAG | 3 cut(s) | 451, 828, 1929 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1728, 1850, 1867, 2376 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1620 |
| BpuEI | CTTGAG | 1 cut(s) | 2802 |
| BpuMI | CCSGG | 1 cut(s) | 1528 |
| Bsa29I | ATCGAT | 2 cut(s) | 27, 393 |
| BsaAI | YACGTR | 1 cut(s) | 2131 |
| BsaBI | GATNNNNATC | 3 cut(s) | 1496, 1611, 1719 |
| BsaHI | GRCGYC | 3 cut(s) | 97, 2534, 2782 |
| BsaWI | WCCGGW | 3 cut(s) | 104, 486, 1402 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 295, 325 |
| Bse1I | ACTGG | 8 cut(s) | 71, 640, 845, 872, 1008, 1147, 1821, 1898 |
| Bse21I | CCTNAGG | 1 cut(s) | 2445 |
| Bse3DI | GCAATG | 1 cut(s) | 1478 |
| Bse8I | GATNNNNATC | 3 cut(s) | 1496, 1611, 1719 |
| BseAI | TCCGGA | 1 cut(s) | 1402 |
| BseBI | CCWGG | 9 cut(s) | 255, 584, 643, 1159, 1215, 1398, 1488, 2708, 2804 |
| BseCI | ATCGAT | 2 cut(s) | 27, 393 |
| BseGI | GGATG | 7 cut(s) | 5, 219, 523, 2011, 2617, 2667, 2883 |
| BseJI | GATNNNNATC | 3 cut(s) | 1496, 1611, 1719 |
| BseMI | GCAATG | 1 cut(s) | 1478 |
| BseMII | CTCAG | 9 cut(s) | 1578, 1611, 1686, 1719, 1841, 1881, 2019, 2367, 2478 |
| BseNI | ACTGG | 8 cut(s) | 71, 640, 845, 872, 1008, 1147, 1821, 1898 |
| BseRI | GAGGAG | 9 cut(s) | 125, 972, 1028, 1592, 1700, 1987, 2270, 2538, 2541 |
| BseSI | GKGCMC | 3 cut(s) | 642, 2338, 2384 |
| BseX3I | CGGCCG | 1 cut(s) | 2790 |
| BseXI | GCAGC | 6 cut(s) | 780, 2251, 2260, 2273, 2482, 2998 |
| BseYI | CCCAGC | 1 cut(s) | 934 |
| Bsh1285I | CGRYCG | 3 cut(s) | 181, 1762, 2793 |
| BshNI | GGYRCC | 5 cut(s) | 250, 1153, 1924, 2333, 2533 |
| BshVI | ATCGAT | 2 cut(s) | 27, 393 |
| BsiEI | CGRYCG | 3 cut(s) | 181, 1762, 2793 |
| BsiHKAI | GWGCWC | 4 cut(s) | 898, 1107, 1626, 1768 |
| BsiHKCI | CYCGRG | 4 cut(s) | 314, 2154, 2649, 2841 |
| BsiSI | CCGG | 5 cut(s) | 105, 487, 947, 1403, 1528 |
| BslFI | GGGAC | 5 cut(s) | 150, 213, 288, 380, 1358 |
| BsmAI | GTCTC | 7 cut(s) | 1574, 1682, 2024, 2350, 2354, 2450, 2654 |
| BsmFI | GGGAC | 5 cut(s) | 150, 213, 288, 380, 1358 |
| BsmI | GAATGC | 5 cut(s) | 59, 578, 1673, 1815, 2974 |
| BsoBI | CYCGRG | 4 cut(s) | 314, 2154, 2649, 2841 |
| Bsp120I | GGGCCC | 2 cut(s) | 638, 2380 |
| Bsp1286I | GDGCHC | 8 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2338, 2384 |
| Bsp13I | TCCGGA | 1 cut(s) | 1402 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1620 |
| Bsp19I | CCATGG | 1 cut(s) | 2410 |
| Bsp68I | TCGCGA | 2 cut(s) | 177, 2350 |
| BspCNI | CTCAG | 9 cut(s) | 1579, 1612, 1687, 1720, 1842, 1880, 2020, 2368, 2479 |
| BspDI | ATCGAT | 2 cut(s) | 27, 393 |
| BspEI | TCCGGA | 1 cut(s) | 1402 |
| BspMAI | CTGCAG | 2 cut(s) | 798, 1879 |
| BspMI | ACCTGC | 2 cut(s) | 238, 1356 |
| BspOI | GCTAGC | 1 cut(s) | 440 |
| BspPI | GGATC | 8 cut(s) | 772, 785, 1607, 1715, 1832, 2195, 2519, 2874 |
| BspQI | GCTCTTC | 1 cut(s) | 1875 |
| BspT107I | GGYRCC | 5 cut(s) | 250, 1153, 1924, 2333, 2533 |
| BsrBI | CCGCTC | 1 cut(s) | 446 |
| BsrDI | GCAATG | 1 cut(s) | 1478 |
| BsrI | ACTGG | 8 cut(s) | 71, 640, 845, 872, 1008, 1147, 1821, 1898 |
| BssNI | GRCGYC | 3 cut(s) | 97, 2534, 2782 |
| BssT1I | CCWWGG | 3 cut(s) | 688, 1508, 2410 |
| Bst2UI | CCWGG | 9 cut(s) | 255, 584, 643, 1159, 1215, 1398, 1488, 2708, 2804 |
| Bst4CI | ACNGT | 4 cut(s) | 307, 1340, 2171, 2368 |
| Bst6I | CTCTTC | 6 cut(s) | 1370, 1631, 1773, 1875, 2118, 2448 |
| BstACI | GRCGYC | 3 cut(s) | 97, 2534, 2782 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2273 |
| BstBAI | YACGTR | 1 cut(s) | 2131 |
| BstC8I | GCNNGC | 6 cut(s) | 438, 1129, 1505, 1750, 2809, 2891 |
| BstDSI | CCRYGG | 1 cut(s) | 2410 |
| BstEII | GGTNACC | 1 cut(s) | 2123 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1853 |
| BstF5I | GGATG | 7 cut(s) | 5, 219, 523, 2011, 2617, 2667, 2883 |
| BstH2I | RGCGCY | 2 cut(s) | 1429, 2537 |
| BstHHI | GCGC | 4 cut(s) | 744, 1055, 1428, 2536 |
| BstMAI | GTCTC | 7 cut(s) | 1574, 1682, 2024, 2350, 2354, 2450, 2654 |
| BstMCI | CGRYCG | 3 cut(s) | 181, 1762, 2793 |
| BstNI | CCWGG | 9 cut(s) | 255, 584, 643, 1159, 1215, 1398, 1488, 2708, 2804 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2366 |
| BstPI | GGTNACC | 1 cut(s) | 2123 |
| BstSFI | CTRYAG | 3 cut(s) | 794, 1440, 1875 |
| BstSLI | GKGCMC | 3 cut(s) | 642, 2338, 2384 |
| BstV1I | GCAGC | 6 cut(s) | 780, 2251, 2260, 2273, 2482, 2998 |
| BstV2I | GAAGAC | 3 cut(s) | 401, 1590, 1698 |
| BstX2I | RGATCY | 4 cut(s) | 777, 1612, 1720, 2200 |
| BstYI | RGATCY | 4 cut(s) | 777, 1612, 1720, 2200 |
| BstZI | CGGCCG | 1 cut(s) | 2790 |
| Bsu15I | ATCGAT | 2 cut(s) | 27, 393 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2445 |
| BsuI | GTATCC | 1 cut(s) | 1485 |
| BsuTUI | ATCGAT | 2 cut(s) | 27, 393 |
| BtgI | CCRYGG | 1 cut(s) | 2410 |
| BtsCI | GGATG | 7 cut(s) | 5, 219, 523, 2011, 2617, 2667, 2883 |
| BtsI | GCAGTG | 3 cut(s) | 657, 1734, 1872 |
| BtsIMutI | CAGTG | 7 cut(s) | 657, 1015, 1083, 1734, 1833, 1872, 2167 |
| BtuMI | TCGCGA | 2 cut(s) | 177, 2350 |
| BveI | ACCTGC | 2 cut(s) | 238, 1356 |
| Cac8I | GCNNGC | 6 cut(s) | 438, 1129, 1505, 1750, 2809, 2891 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1874 |
| CfoI | GCGC | 4 cut(s) | 744, 1055, 1428, 2536 |
| ClaI | ATCGAT | 2 cut(s) | 27, 393 |
| CseI | GACGC | 2 cut(s) | 377, 1472 |
| Csp6I | GTAC | 5 cut(s) | 928, 1154, 1279, 2132, 2250 |
| CviAII | CATG | 6 cut(s) | 79, 566, 1355, 1604, 1712, 2411 |
| CviQI | GTAC | 5 cut(s) | 928, 1154, 1279, 2132, 2250 |
| DinI | GGCGCC | 1 cut(s) | 2535 |
| DraI | TTTAAA | 1 cut(s) | 2871 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1821 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1821 |
| EaeI | YGGCCR | 3 cut(s) | 654, 2175, 2790 |
| EagI | CGGCCG | 1 cut(s) | 2790 |
| Eam1104I | CTCTTC | 6 cut(s) | 1370, 1631, 1773, 1875, 2118, 2448 |
| EarI | CTCTTC | 6 cut(s) | 1370, 1631, 1773, 1875, 2118, 2448 |
| EciI | GGCGGA | 4 cut(s) | 85, 212, 1213, 2876 |
| Ecl136II | GAGCTC | 4 cut(s) | 896, 1105, 1624, 1766 |
| EclXI | CGGCCG | 1 cut(s) | 2790 |
| Eco130I | CCWWGG | 3 cut(s) | 688, 1508, 2410 |
| Eco24I | GRGCYC | 7 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2384 |
| Eco32I | GATATC | 1 cut(s) | 1983 |
| Eco47III | AGCGCT | 1 cut(s) | 1427 |
| Eco52I | CGGCCG | 1 cut(s) | 2790 |
| Eco53kI | GAGCTC | 4 cut(s) | 896, 1105, 1624, 1766 |
| Eco57I | CTGAAG | 3 cut(s) | 2472, 2912, 3039 |
| Eco81I | CCTNAGG | 1 cut(s) | 2445 |
| Eco88I | CYCGRG | 4 cut(s) | 314, 2154, 2649, 2841 |
| Eco91I | GGTNACC | 1 cut(s) | 2123 |
| EcoICRI | GAGCTC | 4 cut(s) | 896, 1105, 1624, 1766 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1853 |
| EcoO109I | RGGNCCY | 9 cut(s) | 164, 639, 1345, 1961, 2108, 2380, 2381, 2645, 2938 |
| EcoO65I | GGTNACC | 1 cut(s) | 2123 |
| EcoRI | GAATTC | 3 cut(s) | 587, 646, 2239 |
| EcoRII | CCWGG | 9 cut(s) | 253, 582, 641, 1157, 1213, 1396, 1486, 2706, 2802 |
| EcoRV | GATATC | 1 cut(s) | 1983 |
| EcoT14I | CCWWGG | 3 cut(s) | 688, 1508, 2410 |
| EcoT38I | GRGCYC | 7 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2384 |
| EgeI | GGCGCC | 1 cut(s) | 2535 |
| EheI | GGCGCC | 1 cut(s) | 2535 |
| ErhI | CCWWGG | 3 cut(s) | 688, 1508, 2410 |
| FaeI | CATG | 6 cut(s) | 82, 569, 1358, 1607, 1715, 2414 |
| FaqI | GGGAC | 5 cut(s) | 150, 213, 288, 380, 1358 |
| FatI | CATG | 6 cut(s) | 78, 565, 1354, 1603, 1711, 2410 |
| FauI | CCCGC | 6 cut(s) | 815, 1218, 1461, 2098, 2867, 2886 |
| FblI | GTMKAC | 3 cut(s) | 94, 1220, 1296 |
| FokI | GGATG | 6 cut(s) | 206, 530, 2018, 2624, 2654, 2870 |
| FriOI | GRGCYC | 7 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2384 |
| FspBI | CTAG | 6 cut(s) | 147, 423, 437, 969, 1509, 2081 |
| GlaI | GCGC | 4 cut(s) | 743, 1054, 1427, 2535 |
| GsaI | CCCAGC | 1 cut(s) | 938 |
| GsuI | CTGGAG | 3 cut(s) | 451, 828, 1929 |
| HaeII | RGCGCY | 2 cut(s) | 1429, 2537 |
| HapII | CCGG | 5 cut(s) | 105, 487, 947, 1403, 1528 |
| HgaI | GACGC | 2 cut(s) | 377, 1472 |
| HhaI | GCGC | 4 cut(s) | 744, 1055, 1428, 2536 |
| Hin1I | GRCGYC | 3 cut(s) | 97, 2534, 2782 |
| Hin1II | CATG | 6 cut(s) | 82, 569, 1358, 1607, 1715, 2414 |
| Hin6I | GCGC | 4 cut(s) | 742, 1053, 1426, 2534 |
| HinP1I | GCGC | 4 cut(s) | 742, 1053, 1426, 2534 |
| HincII | GTYRAC | 1 cut(s) | 1297 |
| HindII | GTYRAC | 1 cut(s) | 1297 |
| HpaII | CCGG | 5 cut(s) | 105, 487, 947, 1403, 1528 |
| HphI | GGTGA | 6 cut(s) | 269, 373, 2107, 2135, 2290, 2426 |
| Hpy166II | GTNNAC | 7 cut(s) | 95, 1013, 1221, 1242, 1297, 1336, 2417 |
| Hpy8I | GTNNAC | 7 cut(s) | 95, 1013, 1221, 1242, 1297, 1336, 2417 |
| Hpy99I | CGWCG | 7 cut(s) | 822, 1053, 1301, 1636, 1778, 2372, 2784 |
| HpyAV | CCTTC | 5 cut(s) | 276, 781, 1479, 2610, 2707 |
| HpyCH4III | ACNGT | 4 cut(s) | 307, 1340, 2171, 2368 |
| HpyCH4IV | ACGT | 4 cut(s) | 97, 882, 2130, 2782 |
| HpySE526I | ACGT | 4 cut(s) | 97, 882, 2130, 2782 |
| Hsp92I | GRCGYC | 3 cut(s) | 97, 2534, 2782 |
| Hsp92II | CATG | 6 cut(s) | 82, 569, 1358, 1607, 1715, 2414 |
| HspAI | GCGC | 4 cut(s) | 742, 1053, 1426, 2534 |
| KasI | GGCGCC | 1 cut(s) | 2533 |
| KflI | GGGWCCC | 1 cut(s) | 164 |
| Kpn2I | TCCGGA | 1 cut(s) | 1402 |
| KpnI | GGTACC | 1 cut(s) | 1157 |
| LguI | GCTCTTC | 1 cut(s) | 1875 |
| LmnI | GCTCC | 4 cut(s) | 725, 901, 1530, 2404 |
| Lsp1109I | GCAGC | 6 cut(s) | 780, 2251, 2260, 2273, 2482, 2998 |
| LweI | GCATC | 3 cut(s) | 14, 1916, 1960 |
| MaeI | CTAG | 6 cut(s) | 147, 423, 437, 969, 1509, 2081 |
| MaeII | ACGT | 4 cut(s) | 97, 882, 2130, 2782 |
| MaeIII | GTNAC | 4 cut(s) | 307, 1097, 2123, 2296 |
| MbiI | CCGCTC | 1 cut(s) | 446 |
| MflI | RGATCY | 4 cut(s) | 777, 1612, 1720, 2200 |
| MhlI | GDGCHC | 8 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2338, 2384 |
| MlsI | TGGCCA | 2 cut(s) | 656, 2177 |
| MluNI | TGGCCA | 2 cut(s) | 656, 2177 |
| Mly113I | GGCGCC | 1 cut(s) | 2534 |
| MlyI | GAGTC | 8 cut(s) | 166, 1243, 1604, 1712, 1988, 2688, 2716, 2741 |
| MmeI | TCCRAC | 5 cut(s) | 495, 648, 2228, 2565, 2940 |
| Mox20I | TGGCCA | 2 cut(s) | 656, 2177 |
| MroI | TCCGGA | 1 cut(s) | 1402 |
| MscI | TGGCCA | 2 cut(s) | 656, 2177 |
| MseI | TTAA | 1 cut(s) | 2870 |
| Msp20I | TGGCCA | 2 cut(s) | 656, 2177 |
| MspA1I | CMGCKG | 1 cut(s) | 2264 |
| MspI | CCGG | 5 cut(s) | 105, 487, 947, 1403, 1528 |
| Mva1269I | GAATGC | 5 cut(s) | 59, 578, 1673, 1815, 2974 |
| MvaI | CCWGG | 9 cut(s) | 255, 584, 643, 1159, 1215, 1398, 1488, 2708, 2804 |
| NarI | GGCGCC | 1 cut(s) | 2534 |
| NciI | CCSGG | 1 cut(s) | 1528 |
| NcoI | CCATGG | 1 cut(s) | 2410 |
| NheI | GCTAGC | 1 cut(s) | 436 |
| NlaIII | CATG | 6 cut(s) | 82, 569, 1358, 1607, 1715, 2414 |
| NmuCI | GTSAC | 3 cut(s) | 307, 2123, 2296 |
| NruI | TCGCGA | 2 cut(s) | 177, 2350 |
| PaeR7I | CTCGAG | 2 cut(s) | 2154, 2841 |
| PaqCI | CACCTGC | 1 cut(s) | 238 |
| PciSI | GCTCTTC | 1 cut(s) | 1875 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 94, 1296, 1365, 1480 |
| PctI | GAATGC | 5 cut(s) | 59, 578, 1673, 1815, 2974 |
| PfeI | GAWTC | 9 cut(s) | 851, 975, 1172, 1255, 2068, 2086, 2393, 2438, 2562 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 2173 |
| PfoI | TCCNGGA | 1 cut(s) | 582 |
| Ple19I | CGATCG | 2 cut(s) | 181, 1762 |
| PleI | GAGTC | 8 cut(s) | 165, 1242, 1603, 1711, 1988, 2687, 2716, 2741 |
| PluTI | GGCGCC | 1 cut(s) | 2537 |
| PpsI | GAGTC | 8 cut(s) | 165, 1242, 1603, 1711, 1988, 2687, 2716, 2741 |
| Ppu21I | YACGTR | 1 cut(s) | 2131 |
| PpuMI | RGGWCCY | 5 cut(s) | 164, 1345, 1961, 2645, 2938 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2366 |
| Psp124BI | GAGCTC | 4 cut(s) | 898, 1107, 1626, 1768 |
| Psp5II | RGGWCCY | 5 cut(s) | 164, 1345, 1961, 2645, 2938 |
| Psp6I | CCWGG | 9 cut(s) | 253, 582, 641, 1157, 1213, 1396, 1486, 2706, 2802 |
| PspEI | GGTNACC | 1 cut(s) | 2123 |
| PspFI | CCCAGC | 1 cut(s) | 934 |
| PspGI | CCWGG | 9 cut(s) | 253, 582, 641, 1157, 1213, 1396, 1486, 2706, 2802 |
| PspOMI | GGGCCC | 2 cut(s) | 638, 2380 |
| PspPPI | RGGWCCY | 5 cut(s) | 164, 1345, 1961, 2645, 2938 |
| PstI | CTGCAG | 2 cut(s) | 798, 1879 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1874 |
| PsuI | RGATCY | 4 cut(s) | 777, 1612, 1720, 2200 |
| PvuI | CGATCG | 2 cut(s) | 181, 1762 |
| PvuII | CAGCTG | 1 cut(s) | 2264 |
| RruI | TCGCGA | 2 cut(s) | 177, 2350 |
| RsaI | GTAC | 5 cut(s) | 929, 1155, 1280, 2133, 2251 |
| RsaNI | GTAC | 5 cut(s) | 928, 1154, 1279, 2132, 2250 |
| SacI | GAGCTC | 4 cut(s) | 898, 1107, 1626, 1768 |
| SalI | GTCGAC | 1 cut(s) | 1295 |
| SapI | GCTCTTC | 1 cut(s) | 1875 |
| SaqAI | TTAA | 1 cut(s) | 2870 |
| SchI | GAGTC | 8 cut(s) | 166, 1243, 1604, 1712, 1988, 2688, 2716, 2741 |
| SduI | GDGCHC | 8 cut(s) | 642, 898, 1036, 1107, 1626, 1768, 2338, 2384 |
| SfaNI | GCATC | 3 cut(s) | 14, 1916, 1960 |
| SfcI | CTRYAG | 3 cut(s) | 794, 1440, 1875 |
| SfoI | GGCGCC | 1 cut(s) | 2535 |
| Sfr274I | CTCGAG | 2 cut(s) | 2154, 2841 |
| SlaI | CTCGAG | 2 cut(s) | 2154, 2841 |
| SmlI | CTYRAG | 3 cut(s) | 2154, 2817, 2841 |
| SmoI | CTYRAG | 3 cut(s) | 2154, 2817, 2841 |
| SspDI | GGCGCC | 1 cut(s) | 2533 |
| SspMI | CTAG | 6 cut(s) | 147, 423, 437, 969, 1509, 2081 |
| SstI | GAGCTC | 4 cut(s) | 898, 1107, 1626, 1768 |
| StyI | CCWWGG | 3 cut(s) | 688, 1508, 2410 |
| TaaI | ACNGT | 4 cut(s) | 307, 1340, 2171, 2368 |
| TaiI | ACGT | 4 cut(s) | 100, 885, 2133, 2785 |
| TatI | WGTACW | 1 cut(s) | 927 |
| TauI | GCSGC | 9 cut(s) | 59, 443, 446, 879, 2042, 2078, 2545, 2795, 3033 |
| TfiI | GAWTC | 9 cut(s) | 851, 975, 1172, 1255, 2068, 2086, 2393, 2438, 2562 |
| Tru1I | TTAA | 1 cut(s) | 2870 |
| Tru9I | TTAA | 1 cut(s) | 2870 |
| TscAI | CASTG | 7 cut(s) | 664, 1015, 1090, 1741, 1840, 1879, 2174 |
| TseFI | GTSAC | 3 cut(s) | 307, 2123, 2296 |
| TseI | GCWGC | 6 cut(s) | 793, 2261, 2264, 2273, 2495, 3011 |
| Tsp45I | GTSAC | 3 cut(s) | 307, 2123, 2296 |
| TspDTI | ATGAA | 8 cut(s) | 54, 1623, 1731, 1812, 2022, 2493, 2775, 2889 |
| TspGWI | ACGGA | 1 cut(s) | 1811 |
| TspRI | CASTG | 7 cut(s) | 664, 1015, 1090, 1741, 1840, 1879, 2174 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 2173 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1853 |
| XapI | RAATTY | 5 cut(s) | 587, 602, 646, 1783, 2239 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1063 |
| XhoI | CTCGAG | 2 cut(s) | 2154, 2841 |
| XmaJI | CCTAGG | 1 cut(s) | 1508 |
| XmiI | GTMKAC | 3 cut(s) | 94, 1220, 1296 |
| XspI | CTAG | 6 cut(s) | 147, 423, 437, 969, 1509, 2081 |
| ZraI | GACGTC | 2 cut(s) | 98, 2783 |