Belongs to the tRNA nucleotidyltransferase poly(A) polymerase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 482, 1659 |
| AatII | GACGTC | 3 cut(s) | 553, 628, 2545 |
| Acc16I | TGCGCA | 1 cut(s) | 1264 |
| AccB1I | GGYRCC | 2 cut(s) | 1762, 2093 |
| AccB7I | CCANNNNNTGG | 5 cut(s) | 701, 758, 940, 964, 1348 |
| AclWI | GGATC | 3 cut(s) | 288, 1553, 2279 |
| AcoI | YGGCCR | 5 cut(s) | 613, 655, 808, 829, 1450 |
| AcsI | RAATTY | 9 cut(s) | 118, 728, 854, 925, 1117, 1127, 1195, 1275, 1333 |
| AcuI | CTGAAG | 2 cut(s) | 2232, 2799 |
| AcvI | CACGTG | 1 cut(s) | 2860 |
| AcyI | GRCGYC | 4 cut(s) | 550, 625, 1282, 2542 |
| AfaI | GTAC | 3 cut(s) | 1304, 1971, 2011 |
| AflII | CTTAAG | 1 cut(s) | 431 |
| AhlI | ACTAGT | 1 cut(s) | 2834 |
| AjnI | CCWGG | 5 cut(s) | 492, 1447, 2466, 2562, 2796 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 411, 443, 825, 857 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 2628, 2649, 2660, 2681 |
| Alw21I | GWGCWC | 1 cut(s) | 1606 |
| Alw26I | GTCTC | 8 cut(s) | 188, 319, 840, 1520, 1862, 2114, 2414, 2602 |
| AlwI | GGATC | 3 cut(s) | 288, 1553, 2279 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1490, 1712 |
| Ama87I | CYCGRG | 3 cut(s) | 1992, 2568, 2601 |
| ApaI | GGGCCC | 1 cut(s) | 587 |
| ApeKI | GCWGC | 8 cut(s) | 20, 23, 26, 2024, 2033, 2488, 2771, 2846 |
| ApoI | RAATTY | 9 cut(s) | 118, 728, 854, 925, 1117, 1127, 1195, 1275, 1333 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 839, 871 |
| AspLEI | GCGC | 2 cut(s) | 14, 1265 |
| AsuC2I | CCSGG | 2 cut(s) | 1474, 1501 |
| AsuHPI | GGTGA | 6 cut(s) | 251, 407, 422, 466, 1945, 2186 |
| AvaI | CYCGRG | 3 cut(s) | 1992, 2568, 2601 |
| AvaII | GGWCC | 7 cut(s) | 163, 1657, 1668, 1799, 2405, 2673, 2698 |
| AxyI | CCTNAGG | 4 cut(s) | 225, 2205, 2409, 2453 |
| BaeGI | GKGCMC | 2 cut(s) | 587, 2098 |
| BalI | TGGCCA | 5 cut(s) | 615, 657, 810, 831, 1452 |
| BanI | GGYRCC | 2 cut(s) | 1762, 2093 |
| BanII | GRGCYC | 3 cut(s) | 587, 1606, 2144 |
| BauI | CACGAG | 2 cut(s) | 825, 1109 |
| BbrPI | CACGTG | 1 cut(s) | 2860 |
| BbsI | GAAGAC | 2 cut(s) | 1239, 1536 |
| Bbv12I | GWGCWC | 1 cut(s) | 1606 |
| BbvCI | CCTCAGC | 3 cut(s) | 1566, 1705, 2136 |
| BbvI | GCAGC | 8 cut(s) | 7, 10, 13, 2011, 2020, 2500, 2758, 2858 |
| BccI | CCATC | 5 cut(s) | 1224, 1265, 1780, 2062, 2651 |
| BceAI | ACGGC | 1 cut(s) | 113 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2819, 2853 |
| BciT130I | CCWGG | 5 cut(s) | 494, 1449, 2468, 2564, 2798 |
| BciVI | GTATCC | 2 cut(s) | 773, 1074 |
| BclI | TGATCA | 5 cut(s) | 343, 367, 412, 520, 712 |
| BcnI | CCSGG | 2 cut(s) | 1474, 1501 |
| BcoDI | GTCTC | 8 cut(s) | 188, 319, 840, 1520, 1862, 2114, 2414, 2602 |
| BcuI | ACTAGT | 1 cut(s) | 2834 |
| BfaI | CTAG | 8 cut(s) | 470, 509, 975, 1251, 1698, 1736, 1919, 2835 |
| BfmI | CTRYAG | 6 cut(s) | 27, 207, 1713, 1782, 2289, 2486 |
| BfrI | CTTAAG | 1 cut(s) | 431 |
| BfuI | GTATCC | 2 cut(s) | 773, 1074 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 2825 |
| Bme1390I | CCNGG | 7 cut(s) | 494, 1449, 1474, 1501, 2468, 2564, 2798 |
| Bme18I | GGWCC | 7 cut(s) | 163, 1657, 1668, 1799, 2405, 2673, 2698 |
| BmeT110I | CYCGRG | 3 cut(s) | 1992, 2568, 2601 |
| BmiI | GGNNCC | 5 cut(s) | 585, 1764, 1947, 2095, 2406 |
| BmrFI | CCNGG | 7 cut(s) | 494, 1449, 1474, 1501, 2468, 2564, 2798 |
| BmsI | GCATC | 4 cut(s) | 363, 1754, 1798, 2563 |
| BoxI | GACNNNNGTC | 1 cut(s) | 2126 |
| BpiI | GAAGAC | 2 cut(s) | 1239, 1536 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 136, 168 |
| Bpu10I | CCTNAGC | 4 cut(s) | 1566, 1688, 1705, 2136 |
| BpuEI | CTTGAG | 1 cut(s) | 2562 |
| BpuMI | CCSGG | 2 cut(s) | 1474, 1501 |
| BsaAI | YACGTR | 3 cut(s) | 358, 1969, 2860 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1557 |
| BsaHI | GRCGYC | 4 cut(s) | 550, 625, 1282, 2542 |
| BsaJI | CCNNGG | 7 cut(s) | 610, 652, 1241, 1836, 2013, 2170, 2562 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1292, 1322 |
| Bse1I | ACTGG | 4 cut(s) | 241, 412, 550, 1659 |
| Bse21I | CCTNAGG | 4 cut(s) | 225, 2205, 2409, 2453 |
| Bse3DI | GCAATG | 1 cut(s) | 1653 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1557 |
| BseBI | CCWGG | 5 cut(s) | 494, 1449, 2468, 2564, 2798 |
| BseDI | CCNNGG | 7 cut(s) | 610, 652, 1241, 1836, 2013, 2170, 2562 |
| BseGI | GGATG | 4 cut(s) | 1849, 2377, 2578, 2643 |
| BseJI | GATNNNNATC | 1 cut(s) | 1557 |
| BseMI | GCAATG | 1 cut(s) | 1653 |
| BseNI | ACTGG | 4 cut(s) | 241, 412, 550, 1659 |
| BseRI | GAGGAG | 7 cut(s) | 1538, 1825, 1955, 2030, 2286, 2301, 2346 |
| BseSI | GKGCMC | 2 cut(s) | 587, 2098 |
| BseXI | GCAGC | 8 cut(s) | 7, 10, 13, 2011, 2020, 2500, 2758, 2858 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1600 |
| BshNI | GGYRCC | 2 cut(s) | 1762, 2093 |
| BsiEI | CGRYCG | 1 cut(s) | 1600 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1606 |
| BsiHKCI | CYCGRG | 3 cut(s) | 1992, 2568, 2601 |
| BsiSI | CCGG | 2 cut(s) | 1474, 1500 |
| BslFI | GGGAC | 2 cut(s) | 2065, 2230 |
| BsmAI | GTCTC | 8 cut(s) | 188, 319, 840, 1520, 1862, 2114, 2414, 2602 |
| BsmFI | GGGAC | 2 cut(s) | 2065, 2230 |
| BsmI | GAATGC | 2 cut(s) | 85, 2734 |
| BsoBI | CYCGRG | 3 cut(s) | 1992, 2568, 2601 |
| Bsp120I | GGGCCC | 1 cut(s) | 583 |
| Bsp1286I | GDGCHC | 4 cut(s) | 587, 1606, 2098, 2144 |
| Bsp19I | CCATGG | 3 cut(s) | 652, 1241, 2170 |
| Bsp68I | TCGCGA | 2 cut(s) | 177, 2394 |
| BspHI | TCATGA | 2 cut(s) | 517, 556 |
| BspLI | GGNNCC | 5 cut(s) | 585, 1764, 1947, 2095, 2406 |
| BspMAI | CTGCAG | 3 cut(s) | 31, 1717, 2490 |
| BspPI | GGATC | 3 cut(s) | 288, 1553, 2279 |
| BspQI | GCTCTTC | 1 cut(s) | 1713 |
| BspT107I | GGYRCC | 2 cut(s) | 1762, 2093 |
| BspTI | CTTAAG | 1 cut(s) | 431 |
| BsrDI | GCAATG | 1 cut(s) | 1653 |
| BsrI | ACTGG | 4 cut(s) | 241, 412, 550, 1659 |
| BssECI | CCNNGG | 7 cut(s) | 610, 652, 1241, 1836, 2013, 2170, 2562 |
| BssNI | GRCGYC | 4 cut(s) | 550, 625, 1282, 2542 |
| BssSI | CACGAG | 2 cut(s) | 825, 1109 |
| BssT1I | CCWWGG | 4 cut(s) | 610, 652, 1241, 2170 |
| Bst2BI | CACGAG | 2 cut(s) | 825, 1109 |
| Bst2UI | CCWGG | 5 cut(s) | 494, 1449, 2468, 2564, 2798 |
| Bst4CI | ACNGT | 5 cut(s) | 851, 1032, 2056, 2087, 2128 |
| Bst6I | CTCTTC | 5 cut(s) | 174, 270, 1611, 1713, 1956 |
| BstACI | GRCGYC | 4 cut(s) | 550, 625, 1282, 2542 |
| BstAFI | CTTAAG | 1 cut(s) | 431 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2033 |
| BstBAI | YACGTR | 3 cut(s) | 358, 1969, 2860 |
| BstC8I | GCNNGC | 6 cut(s) | 85, 1368, 1588, 2536, 2651, 2893 |
| BstDSI | CCRYGG | 3 cut(s) | 652, 1241, 2170 |
| BstF5I | GGATG | 4 cut(s) | 1849, 2377, 2578, 2643 |
| BstHHI | GCGC | 2 cut(s) | 14, 1265 |
| BstMAI | GTCTC | 8 cut(s) | 188, 319, 840, 1520, 1862, 2114, 2414, 2602 |
| BstMCI | CGRYCG | 1 cut(s) | 1600 |
| BstNI | CCWGG | 5 cut(s) | 494, 1449, 2468, 2564, 2798 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 2126 |
| BstSCI | CCNGG | 7 cut(s) | 492, 1447, 1472, 1499, 2466, 2562, 2796 |
| BstSFI | CTRYAG | 6 cut(s) | 27, 207, 1713, 1782, 2289, 2486 |
| BstSLI | GKGCMC | 2 cut(s) | 587, 2098 |
| BstV1I | GCAGC | 8 cut(s) | 7, 10, 13, 2011, 2020, 2500, 2758, 2858 |
| BstV2I | GAAGAC | 2 cut(s) | 1239, 1536 |
| BstX2I | RGATCY | 1 cut(s) | 1558 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 358 |
| BstYI | RGATCY | 1 cut(s) | 1558 |
| Bsu36I | CCTNAGG | 4 cut(s) | 225, 2205, 2409, 2453 |
| BsuI | GTATCC | 2 cut(s) | 773, 1074 |
| BtgI | CCRYGG | 3 cut(s) | 652, 1241, 2170 |
| BtsCI | GGATG | 4 cut(s) | 1849, 2377, 2578, 2643 |
| BtsI | GCAGTG | 3 cut(s) | 1572, 1710, 2303 |
| BtsIMutI | CAGTG | 6 cut(s) | 234, 339, 1028, 1572, 1710, 2303 |
| BtuMI | TCGCGA | 2 cut(s) | 177, 2394 |
| Cac8I | GCNNGC | 6 cut(s) | 85, 1368, 1588, 2536, 2651, 2893 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1490, 1712 |
| CciI | TCATGA | 2 cut(s) | 517, 556 |
| CfoI | GCGC | 2 cut(s) | 14, 1265 |
| CseI | GACGC | 2 cut(s) | 26, 1290 |
| Csp6I | GTAC | 3 cut(s) | 1303, 1970, 2010 |
| CviQI | GTAC | 3 cut(s) | 1303, 1970, 2010 |
| DraI | TTTAAA | 2 cut(s) | 1315, 2631 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 482, 1659 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 482, 1659 |
| EaeI | YGGCCR | 5 cut(s) | 613, 655, 808, 829, 1450 |
| Eam1104I | CTCTTC | 5 cut(s) | 174, 270, 1611, 1713, 1956 |
| EarI | CTCTTC | 5 cut(s) | 174, 270, 1611, 1713, 1956 |
| EciI | GGCGGA | 1 cut(s) | 2636 |
| Ecl136II | GAGCTC | 1 cut(s) | 1604 |
| Eco130I | CCWWGG | 4 cut(s) | 610, 652, 1241, 2170 |
| Eco24I | GRGCYC | 3 cut(s) | 587, 1606, 2144 |
| Eco32I | GATATC | 1 cut(s) | 106 |
| Eco47I | GGWCC | 7 cut(s) | 163, 1657, 1668, 1799, 2405, 2673, 2698 |
| Eco53kI | GAGCTC | 1 cut(s) | 1604 |
| Eco57I | CTGAAG | 2 cut(s) | 2232, 2799 |
| Eco72I | CACGTG | 1 cut(s) | 2860 |
| Eco81I | CCTNAGG | 4 cut(s) | 225, 2205, 2409, 2453 |
| Eco88I | CYCGRG | 3 cut(s) | 1992, 2568, 2601 |
| EcoICRI | GAGCTC | 1 cut(s) | 1604 |
| EcoO109I | RGGNCCY | 6 cut(s) | 229, 583, 1799, 1946, 2405, 2698 |
| EcoRI | GAATTC | 2 cut(s) | 118, 1275 |
| EcoRII | CCWGG | 5 cut(s) | 492, 1447, 2466, 2562, 2796 |
| EcoRV | GATATC | 1 cut(s) | 106 |
| EcoT14I | CCWWGG | 4 cut(s) | 610, 652, 1241, 2170 |
| EcoT38I | GRGCYC | 3 cut(s) | 587, 1606, 2144 |
| ErhI | CCWWGG | 4 cut(s) | 610, 652, 1241, 2170 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 84, 116 |
| FaqI | GGGAC | 2 cut(s) | 2065, 2230 |
| FauI | CCCGC | 6 cut(s) | 2042, 2442, 2543, 2627, 2646, 2884 |
| FbaI | TGATCA | 5 cut(s) | 343, 367, 412, 520, 712 |
| FokI | GGATG | 4 cut(s) | 1856, 2384, 2585, 2630 |
| FriOI | GRGCYC | 3 cut(s) | 587, 1606, 2144 |
| FspAI | RTGCGCAY | 1 cut(s) | 1264 |
| FspBI | CTAG | 8 cut(s) | 470, 509, 975, 1251, 1698, 1736, 1919, 2835 |
| FspI | TGCGCA | 1 cut(s) | 1264 |
| GlaI | GCGC | 2 cut(s) | 13, 1264 |
| HapII | CCGG | 2 cut(s) | 1474, 1500 |
| HgaI | GACGC | 2 cut(s) | 26, 1290 |
| HhaI | GCGC | 2 cut(s) | 14, 1265 |
| Hin1I | GRCGYC | 4 cut(s) | 550, 625, 1282, 2542 |
| Hin6I | GCGC | 2 cut(s) | 12, 1263 |
| HinP1I | GCGC | 2 cut(s) | 12, 1263 |
| HincII | GTYRAC | 2 cut(s) | 640, 879 |
| HindII | GTYRAC | 2 cut(s) | 640, 879 |
| HpaI | GTTAAC | 2 cut(s) | 640, 879 |
| HpaII | CCGG | 2 cut(s) | 1474, 1500 |
| HphI | GGTGA | 6 cut(s) | 251, 407, 422, 466, 1945, 2186 |
| Hpy166II | GTNNAC | 5 cut(s) | 490, 640, 879, 889, 2260 |
| Hpy8I | GTNNAC | 5 cut(s) | 490, 640, 879, 889, 2260 |
| Hpy99I | CGWCG | 5 cut(s) | 488, 1284, 1616, 2132, 2544 |
| HpyAV | CCTTC | 8 cut(s) | 44, 119, 851, 1113, 2207, 2370, 2467, 2711 |
| HpyCH4III | ACNGT | 5 cut(s) | 851, 1032, 2056, 2087, 2128 |
| HpyCH4IV | ACGT | 9 cut(s) | 357, 390, 399, 550, 625, 1968, 2542, 2854, 2859 |
| HpySE526I | ACGT | 9 cut(s) | 357, 390, 399, 550, 625, 1968, 2542, 2854, 2859 |
| Hsp92I | GRCGYC | 4 cut(s) | 550, 625, 1282, 2542 |
| HspAI | GCGC | 2 cut(s) | 12, 1263 |
| Ksp22I | TGATCA | 5 cut(s) | 343, 367, 412, 520, 712 |
| KspAI | GTTAAC | 2 cut(s) | 640, 879 |
| LguI | GCTCTTC | 1 cut(s) | 1713 |
| LmnI | GCTCC | 4 cut(s) | 1210, 1476, 2164, 2299 |
| Lsp1109I | GCAGC | 8 cut(s) | 7, 10, 13, 2011, 2020, 2500, 2758, 2858 |
| LweI | GCATC | 4 cut(s) | 363, 1754, 1798, 2563 |
| MaeI | CTAG | 8 cut(s) | 470, 509, 975, 1251, 1698, 1736, 1919, 2835 |
| MaeII | ACGT | 9 cut(s) | 357, 390, 399, 550, 625, 1968, 2542, 2854, 2859 |
| MaeIII | GTNAC | 4 cut(s) | 533, 1961, 2350, 2855 |
| MfeI | CAATTG | 1 cut(s) | 529 |
| MflI | RGATCY | 1 cut(s) | 1558 |
| MhlI | GDGCHC | 4 cut(s) | 587, 1606, 2098, 2144 |
| MlsI | TGGCCA | 5 cut(s) | 615, 657, 810, 831, 1452 |
| MluNI | TGGCCA | 5 cut(s) | 615, 657, 810, 831, 1452 |
| MlyI | GAGTC | 3 cut(s) | 530, 1550, 2448 |
| MmeI | TCCRAC | 2 cut(s) | 2325, 2700 |
| Mox20I | TGGCCA | 5 cut(s) | 615, 657, 810, 831, 1452 |
| MscI | TGGCCA | 5 cut(s) | 615, 657, 810, 831, 1452 |
| MslI | CAYNNNNRTG | 1 cut(s) | 408 |
| Msp20I | TGGCCA | 5 cut(s) | 615, 657, 810, 831, 1452 |
| MspCI | CTTAAG | 1 cut(s) | 431 |
| MspI | CCGG | 2 cut(s) | 1474, 1500 |
| MspR9I | CCNGG | 7 cut(s) | 494, 1449, 1474, 1501, 2468, 2564, 2798 |
| MunI | CAATTG | 1 cut(s) | 529 |
| Mva1269I | GAATGC | 2 cut(s) | 85, 2734 |
| MvaI | CCWGG | 5 cut(s) | 494, 1449, 2468, 2564, 2798 |
| NciI | CCSGG | 2 cut(s) | 1474, 1501 |
| NcoI | CCATGG | 3 cut(s) | 652, 1241, 2170 |
| NlaIV | GGNNCC | 5 cut(s) | 585, 1764, 1947, 2095, 2406 |
| NmuCI | GTSAC | 3 cut(s) | 533, 1961, 2855 |
| NruI | TCGCGA | 2 cut(s) | 177, 2394 |
| NsbI | TGCGCA | 1 cut(s) | 1264 |
| PaeR7I | CTCGAG | 2 cut(s) | 1992, 2601 |
| PagI | TCATGA | 2 cut(s) | 517, 556 |
| PciSI | GCTCTTC | 1 cut(s) | 1713 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 396 |
| PctI | GAATGC | 2 cut(s) | 85, 2734 |
| PfeI | GAWTC | 8 cut(s) | 139, 242, 437, 1418, 1906, 2000, 2153, 2198 |
| PflMI | CCANNNNNTGG | 5 cut(s) | 701, 758, 940, 964, 1348 |
| PfoI | TCCNGGA | 1 cut(s) | 2466 |
| Ple19I | CGATCG | 1 cut(s) | 1600 |
| PleI | GAGTC | 3 cut(s) | 530, 1549, 2447 |
| PmaCI | CACGTG | 1 cut(s) | 2860 |
| PmlI | CACGTG | 1 cut(s) | 2860 |
| PpsI | GAGTC | 3 cut(s) | 530, 1549, 2447 |
| Ppu21I | YACGTR | 3 cut(s) | 358, 1969, 2860 |
| PpuMI | RGGWCCY | 3 cut(s) | 1799, 2405, 2698 |
| PshAI | GACNNNNGTC | 1 cut(s) | 2126 |
| Psp124BI | GAGCTC | 1 cut(s) | 1606 |
| Psp5II | RGGWCCY | 3 cut(s) | 1799, 2405, 2698 |
| Psp6I | CCWGG | 5 cut(s) | 492, 1447, 2466, 2562, 2796 |
| PspCI | CACGTG | 1 cut(s) | 2860 |
| PspGI | CCWGG | 5 cut(s) | 492, 1447, 2466, 2562, 2796 |
| PspN4I | GGNNCC | 5 cut(s) | 585, 1764, 1947, 2095, 2406 |
| PspOMI | GGGCCC | 1 cut(s) | 583 |
| PspPPI | RGGWCCY | 3 cut(s) | 1799, 2405, 2698 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 384, 416 |
| PstI | CTGCAG | 3 cut(s) | 31, 1717, 2490 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1490, 1712 |
| PsuI | RGATCY | 1 cut(s) | 1558 |
| PvuI | CGATCG | 1 cut(s) | 1600 |
| RruI | TCGCGA | 2 cut(s) | 177, 2394 |
| RsaI | GTAC | 3 cut(s) | 1304, 1971, 2011 |
| RsaNI | GTAC | 3 cut(s) | 1303, 1970, 2010 |
| RseI | CAYNNNNRTG | 1 cut(s) | 408 |
| SacI | GAGCTC | 1 cut(s) | 1606 |
| SapI | GCTCTTC | 1 cut(s) | 1713 |
| SchI | GAGTC | 3 cut(s) | 530, 1550, 2448 |
| ScrFI | CCNGG | 7 cut(s) | 494, 1449, 1474, 1501, 2468, 2564, 2798 |
| SduI | GDGCHC | 4 cut(s) | 587, 1606, 2098, 2144 |
| SfaNI | GCATC | 4 cut(s) | 363, 1754, 1798, 2563 |
| SfcI | CTRYAG | 6 cut(s) | 27, 207, 1713, 1782, 2289, 2486 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 2825 |
| Sfr274I | CTCGAG | 2 cut(s) | 1992, 2601 |
| SinI | GGWCC | 7 cut(s) | 163, 1657, 1668, 1799, 2405, 2673, 2698 |
| SlaI | CTCGAG | 2 cut(s) | 1992, 2601 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 408 |
| SmlI | CTYRAG | 4 cut(s) | 431, 1992, 2577, 2601 |
| SmoI | CTYRAG | 4 cut(s) | 431, 1992, 2577, 2601 |
| SpeI | ACTAGT | 1 cut(s) | 2834 |
| SspMI | CTAG | 8 cut(s) | 470, 509, 975, 1251, 1698, 1736, 1919, 2835 |
| SstI | GAGCTC | 1 cut(s) | 1606 |
| StyD4I | CCNGG | 7 cut(s) | 492, 1447, 1472, 1499, 2466, 2562, 2796 |
| StyI | CCWWGG | 4 cut(s) | 610, 652, 1241, 2170 |
| TaaI | ACNGT | 5 cut(s) | 851, 1032, 2056, 2087, 2128 |
| TaiI | ACGT | 9 cut(s) | 360, 393, 402, 553, 628, 1971, 2545, 2857, 2862 |
| TaqI | TCGA | 9 cut(s) | 844, 1279, 1600, 1614, 1980, 1993, 2130, 2602, 2757 |
| TatI | WGTACW | 1 cut(s) | 1302 |
| TauI | GCSGC | 5 cut(s) | 1880, 1916, 2182, 2555, 2793 |
| TfiI | GAWTC | 8 cut(s) | 139, 242, 437, 1418, 1906, 2000, 2153, 2198 |
| TscAI | CASTG | 6 cut(s) | 241, 346, 1035, 1579, 1717, 2310 |
| TseFI | GTSAC | 3 cut(s) | 533, 1961, 2855 |
| TseI | GCWGC | 8 cut(s) | 20, 23, 26, 2024, 2033, 2488, 2771, 2846 |
| Tsp45I | GTSAC | 3 cut(s) | 533, 1961, 2855 |
| TspGWI | ACGGA | 1 cut(s) | 1649 |
| TspRI | CASTG | 6 cut(s) | 241, 346, 1035, 1579, 1717, 2310 |
| Van91I | CCANNNNNTGG | 5 cut(s) | 701, 758, 940, 964, 1348 |
| Vha464I | CTTAAG | 1 cut(s) | 431 |
| VpaK11BI | GGWCC | 7 cut(s) | 163, 1657, 1668, 1799, 2405, 2673, 2698 |
| XapI | RAATTY | 9 cut(s) | 118, 728, 854, 925, 1117, 1127, 1195, 1275, 1333 |
| XbaI | TCTAGA | 3 cut(s) | 508, 974, 1250 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 404, 650, 1019 |
| XhoI | CTCGAG | 2 cut(s) | 1992, 2601 |
| XspI | CTAG | 8 cut(s) | 470, 509, 975, 1251, 1698, 1736, 1919, 2835 |
| ZraI | GACGTC | 3 cut(s) | 551, 626, 2543 |