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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 3 cut(s) | 91, 108, 137 |
| AciI | CCGC | 1 cut(s) | 106 |
| AcoI | YGGCCR | 1 cut(s) | 82 |
| AjnI | CCWGG | 1 cut(s) | 69 |
| AluBI | AGCT | 2 cut(s) | 153, 160 |
| AluI | AGCT | 2 cut(s) | 153, 160 |
| Ama87I | CYCGRG | 1 cut(s) | 62 |
| AoxI | GGCC | 2 cut(s) | 82, 198 |
| AspLEI | GCGC | 3 cut(s) | 91, 93, 110 |
| AspS9I | GGNCC | 1 cut(s) | 67 |
| AsuC2I | CCSGG | 3 cut(s) | 63, 64, 86 |
| AvaI | CYCGRG | 1 cut(s) | 62 |
| AvaII | GGWCC | 1 cut(s) | 67 |
| BciT130I | CCWGG | 1 cut(s) | 71 |
| BcnI | CCSGG | 3 cut(s) | 63, 64, 86 |
| BfaI | CTAG | 1 cut(s) | 202 |
| Bme1390I | CCNGG | 4 cut(s) | 63, 64, 71, 86 |
| Bme18I | GGWCC | 1 cut(s) | 67 |
| BmeT110I | CYCGRG | 1 cut(s) | 62 |
| BmgT120I | GGNCC | 1 cut(s) | 67 |
| BmiI | GGNNCC | 1 cut(s) | 68 |
| BmrFI | CCNGG | 4 cut(s) | 63, 64, 71, 86 |
| BpuEI | CTTGAG | 1 cut(s) | 65 |
| BpuMI | CCSGG | 3 cut(s) | 63, 64, 86 |
| BsaJI | CCNNGG | 3 cut(s) | 61, 62, 63 |
| BseBI | CCWGG | 1 cut(s) | 71 |
| BseDI | CCNNGG | 3 cut(s) | 61, 62, 63 |
| BsePI | GCGCGC | 1 cut(s) | 89 |
| Bsh1236I | CGCG | 3 cut(s) | 91, 108, 137 |
| Bsh1285I | CGRYCG | 1 cut(s) | 141 |
| BshFI | GGCC | 2 cut(s) | 84, 200 |
| BsiEI | CGRYCG | 1 cut(s) | 141 |
| BsiHKCI | CYCGRG | 1 cut(s) | 62 |
| BsiSI | CCGG | 2 cut(s) | 63, 85 |
| BslFI | GGGAC | 1 cut(s) | 80 |
| BsmFI | GGGAC | 1 cut(s) | 80 |
| BsnI | GGCC | 2 cut(s) | 84, 200 |
| BsoBI | CYCGRG | 1 cut(s) | 62 |
| Bsp68I | TCGCGA | 1 cut(s) | 137 |
| BspACI | CCGC | 1 cut(s) | 106 |
| BspANI | GGCC | 2 cut(s) | 84, 200 |
| BspFNI | CGCG | 3 cut(s) | 91, 108, 137 |
| BspLI | GGNNCC | 1 cut(s) | 68 |
| BssECI | CCNNGG | 3 cut(s) | 61, 62, 63 |
| BssHII | GCGCGC | 1 cut(s) | 89 |
| Bst2UI | CCWGG | 1 cut(s) | 71 |
| Bst4CI | ACNGT | 2 cut(s) | 13, 80 |
| BstC8I | GCNNGC | 2 cut(s) | 91, 184 |
| BstDEI | CTNAG | 1 cut(s) | 121 |
| BstFNI | CGCG | 3 cut(s) | 91, 108, 137 |
| BstHHI | GCGC | 3 cut(s) | 91, 93, 110 |
| BstMCI | CGRYCG | 1 cut(s) | 141 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 90, 95 |
| BstNI | CCWGG | 1 cut(s) | 71 |
| BstSCI | CCNGG | 4 cut(s) | 61, 62, 69, 84 |
| BstUI | CGCG | 3 cut(s) | 91, 108, 137 |
| BsuRI | GGCC | 2 cut(s) | 84, 200 |
| BtuMI | TCGCGA | 1 cut(s) | 137 |
| Cac8I | GCNNGC | 2 cut(s) | 91, 184 |
| CfoI | GCGC | 3 cut(s) | 91, 93, 110 |
| Cfr13I | GGNCC | 1 cut(s) | 67 |
| Cfr9I | CCCGGG | 1 cut(s) | 62 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 25, 60 |
| CviJI | RGCY | 5 cut(s) | 84, 98, 153, 160, 200 |
| CviKI_1 | RGCY | 5 cut(s) | 84, 98, 153, 160, 200 |
| DdeI | CTNAG | 1 cut(s) | 121 |
| EaeI | YGGCCR | 1 cut(s) | 82 |
| Eco47I | GGWCC | 1 cut(s) | 67 |
| Eco88I | CYCGRG | 1 cut(s) | 62 |
| EcoO109I | RGGNCCY | 1 cut(s) | 67 |
| EcoRII | CCWGG | 1 cut(s) | 69 |
| FaqI | GGGAC | 1 cut(s) | 80 |
| FspBI | CTAG | 1 cut(s) | 202 |
| GlaI | GCGC | 3 cut(s) | 90, 92, 109 |
| HaeIII | GGCC | 2 cut(s) | 84, 200 |
| HapII | CCGG | 2 cut(s) | 63, 85 |
| HhaI | GCGC | 3 cut(s) | 91, 93, 110 |
| Hin6I | GCGC | 3 cut(s) | 89, 91, 108 |
| HinP1I | GCGC | 3 cut(s) | 89, 91, 108 |
| HinfI | GANTC | 2 cut(s) | 31, 132 |
| HpaII | CCGG | 2 cut(s) | 63, 85 |
| Hpy166II | GTNNAC | 1 cut(s) | 103 |
| Hpy188I | TCNGA | 1 cut(s) | 194 |
| Hpy188III | TCNNGA | 2 cut(s) | 44, 136 |
| Hpy8I | GTNNAC | 1 cut(s) | 103 |
| HpyCH4III | ACNGT | 2 cut(s) | 13, 80 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 90, 95 |
| HpyF3I | CTNAG | 1 cut(s) | 121 |
| HspAI | GCGC | 3 cut(s) | 89, 91, 108 |
| LmnI | GCTCC | 1 cut(s) | 172 |
| LpnPI | CCDG | 4 cut(s) | 56, 76, 83, 98 |
| MaeI | CTAG | 1 cut(s) | 202 |
| MaeIII | GTNAC | 1 cut(s) | 13 |
| MlyI | GAGTC | 1 cut(s) | 126 |
| MnlI | CCTC | 1 cut(s) | 51 |
| MspI | CCGG | 2 cut(s) | 63, 85 |
| MspR9I | CCNGG | 4 cut(s) | 63, 64, 71, 86 |
| MvaI | CCWGG | 1 cut(s) | 71 |
| MvnI | CGCG | 3 cut(s) | 91, 108, 137 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 90, 95 |
| NciI | CCSGG | 3 cut(s) | 63, 64, 86 |
| NlaIV | GGNNCC | 1 cut(s) | 68 |
| NruI | TCGCGA | 1 cut(s) | 137 |
| PauI | GCGCGC | 1 cut(s) | 89 |
| PfeI | GAWTC | 1 cut(s) | 31 |
| PleI | GAGTC | 1 cut(s) | 126 |
| PpsI | GAGTC | 1 cut(s) | 126 |
| PpuMI | RGGWCCY | 1 cut(s) | 67 |
| Psp5II | RGGWCCY | 1 cut(s) | 67 |
| Psp6I | CCWGG | 1 cut(s) | 69 |
| PspGI | CCWGG | 1 cut(s) | 69 |
| PspN4I | GGNNCC | 1 cut(s) | 68 |
| PspPI | GGNCC | 1 cut(s) | 67 |
| PspPPI | RGGWCCY | 1 cut(s) | 67 |
| PteI | GCGCGC | 1 cut(s) | 89 |
| RruI | TCGCGA | 1 cut(s) | 137 |
| Sau96I | GGNCC | 1 cut(s) | 67 |
| SchI | GAGTC | 1 cut(s) | 126 |
| ScrFI | CCNGG | 4 cut(s) | 63, 64, 71, 86 |
| SetI | ASST | 4 cut(s) | 43, 72, 155, 162 |
| SinI | GGWCC | 1 cut(s) | 67 |
| SmaI | CCCGGG | 1 cut(s) | 64 |
| SmlI | CTYRAG | 1 cut(s) | 44 |
| SmoI | CTYRAG | 1 cut(s) | 44 |
| SsiI | CCGC | 1 cut(s) | 106 |
| SspMI | CTAG | 1 cut(s) | 202 |
| StyD4I | CCNGG | 4 cut(s) | 61, 62, 69, 84 |
| TaaI | ACNGT | 2 cut(s) | 13, 80 |
| TaqII | GACCGA | 1 cut(s) | 155 |
| TfiI | GAWTC | 1 cut(s) | 31 |
| TspMI | CCCGGG | 1 cut(s) | 62 |
| VpaK11BI | GGWCC | 1 cut(s) | 67 |
| XmaI | CCCGGG | 1 cut(s) | 62 |
| XspI | CTAG | 1 cut(s) | 202 |