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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 379 |
| AccII | CGCG | 1 cut(s) | 196 |
| AciI | CCGC | 4 cut(s) | 194, 221, 227, 365 |
| AclWI | GGATC | 2 cut(s) | 54, 208 |
| AcuI | CTGAAG | 3 cut(s) | 43, 239, 373 |
| AcvI | CACGTG | 1 cut(s) | 434 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 227, 437 |
| AgsI | TTSAA | 3 cut(s) | 9, 264, 452 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 202, 223, 234, 255 |
| AluBI | AGCT | 3 cut(s) | 324, 408, 460 |
| AluI | AGCT | 3 cut(s) | 324, 408, 460 |
| AlwI | GGATC | 2 cut(s) | 54, 208 |
| AoxI | GGCC | 4 cut(s) | 362, 391, 400, 467 |
| AspLEI | GCGC | 1 cut(s) | 71 |
| AspS9I | GGNCC | 3 cut(s) | 247, 272, 362 |
| AsuHPI | GGTGA | 1 cut(s) | 69 |
| AvaII | GGWCC | 2 cut(s) | 247, 272 |
| BbrPI | CACGTG | 1 cut(s) | 434 |
| BccI | CCATC | 2 cut(s) | 152, 225 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 393, 427 |
| BfuAI | ACCTGC | 1 cut(s) | 379 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 399 |
| BisI | GCNGC | 1 cut(s) | 365 |
| BlsI | GCNGC | 1 cut(s) | 366 |
| Bme18I | GGWCC | 2 cut(s) | 247, 272 |
| BmgT120I | GGNCC | 3 cut(s) | 247, 272, 362 |
| BmrI | ACTGGG | 1 cut(s) | 370 |
| BmuI | ACTGGG | 1 cut(s) | 370 |
| BsaAI | YACGTR | 1 cut(s) | 434 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 227, 437 |
| Bse1I | ACTGG | 1 cut(s) | 376 |
| BseGI | GGATG | 1 cut(s) | 217 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 227, 437 |
| BseMII | CTCAG | 1 cut(s) | 411 |
| BseNI | ACTGG | 1 cut(s) | 376 |
| BseRI | GAGGAG | 2 cut(s) | 73, 76 |
| Bsh1236I | CGCG | 1 cut(s) | 196 |
| BshFI | GGCC | 4 cut(s) | 364, 393, 402, 469 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 227, 437 |
| BsmI | GAATGC | 2 cut(s) | 71, 308 |
| BsnI | GGCC | 4 cut(s) | 364, 393, 402, 469 |
| Bsp143I | GATC | 3 cut(s) | 59, 213, 386 |
| BspACI | CCGC | 4 cut(s) | 194, 221, 227, 365 |
| BspANI | GGCC | 4 cut(s) | 364, 393, 402, 469 |
| BspCNI | CTCAG | 1 cut(s) | 410 |
| BspFNI | CGCG | 1 cut(s) | 196 |
| BspMI | ACCTGC | 1 cut(s) | 379 |
| BspPI | GGATC | 2 cut(s) | 54, 208 |
| BsrI | ACTGG | 1 cut(s) | 376 |
| BssMI | GATC | 3 cut(s) | 59, 213, 386 |
| Bst6I | CTCTTC | 1 cut(s) | 19 |
| BstBAI | YACGTR | 1 cut(s) | 434 |
| BstC8I | GCNNGC | 2 cut(s) | 225, 467 |
| BstDEI | CTNAG | 2 cut(s) | 154, 397 |
| BstF5I | GGATG | 1 cut(s) | 217 |
| BstFNI | CGCG | 1 cut(s) | 196 |
| BstHHI | GCGC | 1 cut(s) | 71 |
| BstKTI | GATC | 3 cut(s) | 62, 216, 389 |
| BstMBI | GATC | 3 cut(s) | 59, 213, 386 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 288, 312, 321, 399, 420, 466 |
| BstUI | CGCG | 1 cut(s) | 196 |
| BsuRI | GGCC | 4 cut(s) | 364, 393, 402, 469 |
| BtsCI | GGATG | 1 cut(s) | 217 |
| BveI | ACCTGC | 1 cut(s) | 379 |
| Cac8I | GCNNGC | 2 cut(s) | 225, 467 |
| CfoI | GCGC | 1 cut(s) | 71 |
| Cfr13I | GGNCC | 3 cut(s) | 247, 272, 362 |
| DdeI | CTNAG | 2 cut(s) | 154, 397 |
| DpnI | GATC | 3 cut(s) | 61, 215, 388 |
| DpnII | GATC | 3 cut(s) | 59, 213, 386 |
| DraI | TTTAAA | 1 cut(s) | 205 |
| Eam1104I | CTCTTC | 1 cut(s) | 19 |
| EarI | CTCTTC | 1 cut(s) | 19 |
| EciI | GGCGGA | 1 cut(s) | 210 |
| Eco47I | GGWCC | 2 cut(s) | 247, 272 |
| Eco57I | CTGAAG | 3 cut(s) | 43, 239, 373 |
| Eco72I | CACGTG | 1 cut(s) | 434 |
| EcoO109I | RGGNCCY | 1 cut(s) | 272 |
| FauI | CCCGC | 2 cut(s) | 201, 220 |
| Fnu4HI | GCNGC | 1 cut(s) | 365 |
| FokI | GGATG | 1 cut(s) | 204 |
| Fsp4HI | GCNGC | 1 cut(s) | 365 |
| GlaI | GCGC | 1 cut(s) | 70 |
| GluI | GCNGC | 1 cut(s) | 365 |
| HaeIII | GGCC | 4 cut(s) | 364, 393, 402, 469 |
| HhaI | GCGC | 1 cut(s) | 71 |
| Hin6I | GCGC | 1 cut(s) | 69 |
| HinP1I | GCGC | 1 cut(s) | 69 |
| HinfI | GANTC | 1 cut(s) | 97 |
| HphI | GGTGA | 1 cut(s) | 69 |
| Hpy166II | GTNNAC | 1 cut(s) | 35 |
| Hpy188I | TCNGA | 4 cut(s) | 251, 258, 353, 384 |
| Hpy188III | TCNNGA | 3 cut(s) | 101, 168, 177 |
| Hpy8I | GTNNAC | 1 cut(s) | 35 |
| HpyAV | CCTTC | 1 cut(s) | 145 |
| HpyCH4IV | ACGT | 1 cut(s) | 433 |
| HpyCH4V | TGCA | 3 cut(s) | 45, 315, 465 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 288, 312, 321, 399, 420, 466 |
| HpyF3I | CTNAG | 2 cut(s) | 154, 397 |
| HpySE526I | ACGT | 1 cut(s) | 433 |
| HspAI | GCGC | 1 cut(s) | 69 |
| Kzo9I | GATC | 3 cut(s) | 59, 213, 386 |
| MaeII | ACGT | 1 cut(s) | 433 |
| MalI | GATC | 3 cut(s) | 61, 215, 388 |
| MboI | GATC | 3 cut(s) | 59, 213, 386 |
| MboII | GAAGA | 5 cut(s) | 21, 27, 36, 330, 366 |
| MluCI | AATT | 1 cut(s) | 453 |
| MmeI | TCCRAC | 2 cut(s) | 100, 274 |
| MseI | TTAA | 2 cut(s) | 204, 342 |
| Mva1269I | GAATGC | 2 cut(s) | 71, 308 |
| MvnI | CGCG | 1 cut(s) | 196 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 288, 312, 321, 399, 420, 466 |
| NdeII | GATC | 3 cut(s) | 59, 213, 386 |
| PctI | GAATGC | 2 cut(s) | 71, 308 |
| PfeI | GAWTC | 1 cut(s) | 97 |
| PkrI | GCNGC | 1 cut(s) | 366 |
| PmaCI | CACGTG | 1 cut(s) | 434 |
| PmlI | CACGTG | 1 cut(s) | 434 |
| Ppu21I | YACGTR | 1 cut(s) | 434 |
| PpuMI | RGGWCCY | 1 cut(s) | 272 |
| Psp5II | RGGWCCY | 1 cut(s) | 272 |
| PspCI | CACGTG | 1 cut(s) | 434 |
| PspPI | GGNCC | 3 cut(s) | 247, 272, 362 |
| PspPPI | RGGWCCY | 1 cut(s) | 272 |
| SaqAI | TTAA | 2 cut(s) | 204, 342 |
| SatI | GCNGC | 1 cut(s) | 365 |
| Sau3AI | GATC | 3 cut(s) | 59, 213, 386 |
| Sau96I | GGNCC | 3 cut(s) | 247, 272, 362 |
| SetI | ASST | 9 cut(s) | 83, 127, 249, 277, 326, 373, 410, 436, 462 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 399 |
| SinI | GGWCC | 2 cut(s) | 247, 272 |
| Sse9I | AATT | 1 cut(s) | 453 |
| SsiI | CCGC | 4 cut(s) | 194, 221, 227, 365 |
| TaiI | ACGT | 1 cut(s) | 436 |
| TaqI | TCGA | 2 cut(s) | 100, 268 |
| TasI | AATT | 1 cut(s) | 453 |
| TauI | GCSGC | 1 cut(s) | 367 |
| TfiI | GAWTC | 1 cut(s) | 97 |
| Tru1I | TTAA | 2 cut(s) | 204, 342 |
| Tru9I | TTAA | 2 cut(s) | 204, 342 |
| TspDTI | ATGAA | 2 cut(s) | 28, 223 |
| VpaK11BI | GGWCC | 2 cut(s) | 247, 272 |