Rmu_sc0001461.1_g000058

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001461.1
OG0000214
Physical Location & Seq
Forward (+)
213761 .. 217207
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001461.1_g000058.1.cds

Sequence Viewer

Length: 2910 bp
atggcatccaagaaaccattcgttatcgatcaggcagatgaaatcactgagaaagccgcattcacatggcggactaacatgagtgttcaatgtagaagtcagaccggagaggagataagtcgactgatgggctttggtggcggtgatagtcaagcgagtctgggtcccactcctcgtgatcgtttgccaaataaggttgtggcctattatgggcttcccatcctccgtcccatagcggctctttggcaggtgcctggtgattttagcagttggggaccaaggaggaaagtgggcttctggcctaccatcgctctcgaggaaaaggtttggcagatgagcataactatgctggatgttcttaccatcacggggttgcccgttgactttgacccctatgtgcatggtcagtttgatggcattcaattttctttaagaatggatatggctagtcgggggcatcatagcagatcctacccaatctggcgcgactattactgcacccagcgtgaccatacaggaggtatcgcattcctggaattcggctttgcaaatttatcttttgcatttcttctagcaaacctactgggccatggaattctctggccattgctctctacaatggtacttgcgttggccttgggcaacctgtattggatttatttccctcccttccgtcgcgatgatattccaaaggaacctccagtcgattcccttttggggaactggctttgccgcaacgtaaggtatcaagctcctccctactccgagtgttttatttacttgtacttgctgggagagatgccggacccaaaactagtcatttctaggcgtttccctcctcccctttatgatggctttctgcccagtggacgggatcatagcgaaaaagccctcctgccctttcgtcgcgccatctcctgcttggatattcgtcttgccaccaaccgcgtaagttatgagctctatgcccctaaccacttcgcttgccaatttgggttggcccagttggtaccgtggcctctcgttgattctgccaattactacacctcttggcgaagattagccccacctgggtctgccccctttcagagccagtttactttgatagtgattcccccttgggtcagagcgttgtaccccctcaacgatatcgacgatgattatgctgattggtggaaagaagtgtccgtgaactgttgggacctgccacatgacggactcttcgtcctgatctttcgtggcatgagtagtcctggtccggaggaccgcgagattcttgagcgcttctccaaccctgcagcacaacccccgcgacctattctaccttctcgctcatcgcgtccagggatacaaatccacgtgcctagggcaacagcaataccagaggagactcctgagggagagtcttctcatgatgaagatcccgctgaggccactgctgtctttgcttgcaaaagcgatcgatctcaattcgtcgaagagagttttgaagatgacgaacctctgggaatgcgattggtccgtcagaggactactgacccctctcgcctgagcgaggctagatcctcagccactgcagaagagccaactctctcactagttcaagcatcaactaaccccgtggcgcctcttccgtctcccacatctacagagcatctgcaaggtcctaccattctaataatgatctccgatgatgactcctcggagcatgaaagcgtggaaagccactctgaggcttctgccgcttatgaggacctgatgacgacggagattctcgcggcactaaaggtccaagaggtgaatgaggtgaggcctcttcctcatggagaccacgtaccagatattgacccgacagctcgagaggtaagccactgttggccaatgctttccatttccctttctgatccaaccccgctctctgacatttctgaatctgaccaggattctgtttgtatcgaggaggtggctgcaaacgctgagggtcctatcggcgggacagtcgccccagagatggaaaatgatactgacggtggtggtgccccccaagtctcgcgaataaatgagacaaccatcggagccgagggctctgtgcttgaatctgctccacttgcccatggtgagcctcgagttgagtttccagattctcctgctgctgaagggacagaccaacctgttgaagatgaagaaactgaggaagttgtccacccttttgcattggtggttcgtgatcctgtggcgcctccgcagcctcctcctcctaccagattcgagagattgatgagggtgttggaggtaactcctccttcttctgtggatgaggctaagatggtacttcacgaacatctgggtcctcgggtattggagactgacatcctcccgcgagtcttggaagctctaaggtatcttcgccaggagaaggttttgacttcgtggcaatttagtgctatcgactctctgctaaatgaacttgatatggcccaccgacgtcaatcggccgcgaacacccaggctaacgacgctcaagaggctttgaatagtgtatctcgagaaatggacatctcccgcgatattttaaatgaacgagccatccgcctgcgggaactacaaatccaaaggtctgacttcaaacttcagatcgaggacctcaaagcccatttagcctttgttgagaatgcgattgttgcaaaagaagctcaactcgatgaacctttggctgcttctgaagaaatgggccgcaacctggcccgtatcagagaacgggtcactcaggccgaagctggtgccattgcggcgaaacgccacgtggaggaactttgtttgaaattgagctttgcggaccaatctctgtgggccccctcgaaaaccgttcggtttcctcacatgctattaatcccttcctttaatagctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

969

Amino Acids

108.2

Weight (kDa)

4.83

Isoelectric Point (pI)

54.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000214)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0079611
rosa_multiflora Rmu_co8150358.1_g000001 Rmu_co8306601.1_g000001 Rmu_sc0000032.1_g000018 Rmu_sc0000110.1_g000025 Rmu_sc0000110.1_g000026 Rmu_sc0000126.1_g000018 Rmu_sc0000147.1_g000058 Rmu_sc0000187.1_g000010 Rmu_sc0000240.1_g000061 Rmu_sc0000240.1_g000062 Rmu_sc0000258.1_g000018 Rmu_sc0000258.1_g000020 Rmu_sc0000276.1_g000075 Rmu_sc0000276.1_g000076 Rmu_sc0000356.1_g000007 Rmu_sc0000360.1_g000033 Rmu_sc0000368.1_g000014 Rmu_sc0000368.1_g000026 Rmu_sc0000389.1_g000011 Rmu_sc0000588.1_g000024 Rmu_sc0000595.1_g000060 Rmu_sc0000744.1_g000144 Rmu_sc0000744.1_g000145 Rmu_sc0000770.1_g000053 Rmu_sc0000795.1_g000096 Rmu_sc0000810.1_g000011 Rmu_sc0000935.1_g000066 Rmu_sc0000945.1_g000014 Rmu_sc0000962.1_g000011 Rmu_sc0000962.1_g000023 Rmu_sc0000962.1_g000024 Rmu_sc0001047.1_g000012 Rmu_sc0001094.1_g000015 Rmu_sc0001097.1_g000032 Rmu_sc0001254.1_g000006 Rmu_sc0001264.1_g000026 Rmu_sc0001322.1_g000013 Rmu_sc0001373.1_g000001 Rmu_sc0001424.1_g000002 Rmu_sc0001455.1_g000009 Rmu_sc0001461.1_g000008 Rmu_sc0001461.1_g000058 Rmu_sc0001563.1_g000014 Rmu_sc0001793.1_g000012 Rmu_sc0001793.1_g000013 Rmu_sc0001796.1_g000010 Rmu_sc0001817.1_g000004 Rmu_sc0002141.1_g000011 Rmu_sc0002141.1_g000012 Rmu_sc0002183.1_g000010 Rmu_sc0002183.1_g000015 Rmu_sc0002212.1_g000033 Rmu_sc0002212.1_g000034 Rmu_sc0002263.1_g000049 Rmu_sc0002263.1_g000050 Rmu_sc0002263.1_g000055 Rmu_sc0002263.1_g000056 Rmu_sc0002489.1_g000039 Rmu_sc0002654.1_g000003 Rmu_sc0002680.1_g000036 Rmu_sc0002745.1_g000013 Rmu_sc0002751.1_g000001 Rmu_sc0002769.1_g000013 Rmu_sc0002972.1_g000004 Rmu_sc0002985.1_g000011 Rmu_sc0003199.1_g000002 Rmu_sc0003207.1_g000020 Rmu_sc0003402.1_g000005 Rmu_sc0003500.1_g000009 Rmu_sc0003500.1_g000010 Rmu_sc0003600.1_g000051 Rmu_sc0003809.1_g000025 Rmu_sc0003859.1_g000029 Rmu_sc0003859.1_g000030 Rmu_sc0003994.1_g000025 Rmu_sc0004085.1_g000010 Rmu_sc0004085.1_g000015 Rmu_sc0004165.1_g000061 Rmu_sc0004525.1_g000006 Rmu_sc0004549.1_g000012 Rmu_sc0004598.1_g000035 Rmu_sc0004835.1_g000007 Rmu_sc0004881.1_g000008 Rmu_sc0005325.1_g000004 Rmu_sc0005325.1_g000006 Rmu_sc0005608.1_g000003 Rmu_sc0005747.1_g000026 Rmu_sc0006153.1_g000059 Rmu_sc0006273.1_g000010 Rmu_sc0006737.1_g000002 Rmu_sc0006737.1_g000003 Rmu_sc0006984.1_g000006 Rmu_sc0006984.1_g000007 Rmu_sc0007170.1_g000001 Rmu_sc0007801.1_g000002 Rmu_sc0007992.1_g000008 Rmu_sc0008698.1_g000003 Rmu_sc0010817.1_g000001 Rmu_sc0010817.1_g000021 Rmu_sc0011207.1_g000001 Rmu_sc0012533.1_g000007 Rmu_sc0012742.1_g000008 Rmu_sc0014075.1_g000001 Rmu_sc0017009.1_g000004 Rmu_sc0018430.1_g000002 Rmu_sc0020146.1_g000003 Rmu_sc0021186.1_g000001 Rmu_sc0023004.1_g000001 Rmu_sc0024933.1_g000001 Rmu_sc0026002.1_g000001 Rmu_sc0026989.1_g000005 Rmu_sc0028107.1_g000001 Rmu_sc0028108.1_g000001 Rmu_sc0032899.1_g000001 Rmu_sc0039686.1_g000001 Rmu_ssc0000134.1_g000054 Rmu_ssc0000213.1_g000089 Rmu_ssc0000295.1_g000043 Rmu_ssc0000295.1_g000045 Rmu_ssc0000309.1_g000024 Rmu_ssc0000309.1_g000025 Rmu_ssc0000438.1_g000003 Rmu_ssc0000438.1_g000017 Rmu_ssc0000438.1_g000018
rosa_roxburghii Rroxscaffold_1G00020710 Rroxscaffold_2G00077350 Rroxscaffold_2G00077360 Rroxscaffold_2G00137810 Rroxscaffold_2G00142810 Rroxscaffold_2G00143580 Rroxscaffold_2G00152890 Rroxscaffold_3G00248850 Rroxscaffold_4G00324400 Rroxscaffold_4G00329960 Rroxscaffold_4G00329970 Rroxscaffold_5G00339350 Rroxscaffold_5G00343640 Rroxscaffold_6G00393540 Rroxscaffold_6G00400820 Rroxscaffold_6G00408530 Rroxscaffold_6G00408540 Rroxscaffold_6G00414590 Rroxscaffold_6G00414600 Rroxscaffold_7G00180540 Rroxscaffold_7G00199530 Rroxscaffold_7G00203170 Rroxscaffold_7G00217520
rosa_samantha Rh3DG208400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
AatII GACGTC 1 cut(s) 2488
Acc36I ACCTGC 2 cut(s) 238, 1212
Acc65I GGTACC 1 cut(s) 1009
AccB1I GGYRCC 6 cut(s) 250, 1009, 1624, 2036, 2236, 2780
AccBSI CCGCTC 1 cut(s) 1915
AccI GTMKAC 1 cut(s) 121
AccIII TCCGGA 1 cut(s) 1258
AclWI GGATC 6 cut(s) 462, 882, 1415, 1557, 1898, 2222
AcoI YGGCCR 3 cut(s) 602, 1877, 2493
AcsI RAATTY 3 cut(s) 536, 550, 594
AcuI CTGAAG 3 cut(s) 2175, 2615, 2742
AcvI CACGTG 2 cut(s) 1360, 2803
AcyI GRCGYC 3 cut(s) 1625, 2237, 2485
AfaI GTAC 6 cut(s) 624, 785, 1011, 1136, 1836, 2331
AfeI AGCGCT 1 cut(s) 1283
AgsI TTSAA 9 cut(s) 89, 422, 1490, 1604, 2096, 2177, 2533, 2626, 2821
AhdI GACNNNNNGTC 1 cut(s) 1223
AhlI ACTAGT 2 cut(s) 814, 1597
AjnI CCWGG 9 cut(s) 253, 531, 1069, 1252, 1342, 1938, 2409, 2505, 2739
AjuI GAANNNNNNNTTGG 1 cut(s) 34
AloI GAACNNNNNNTCC 4 cut(s) 2571, 2592, 2603, 2624
AluBI AGCT 8 cut(s) 752, 961, 1856, 2393, 2693, 2777, 2829, 2907
AluI AGCT 8 cut(s) 752, 961, 1856, 2393, 2693, 2777, 2829, 2907
Alw21I GWGCWC 1 cut(s) 963
Alw26I GTCTC 6 cut(s) 1382, 1641, 1821, 2053, 2057, 2357
AlwI GGATC 6 cut(s) 462, 882, 1415, 1557, 1898, 2222
AlwNI CAGNNNCTG 1 cut(s) 1574
Ama87I CYCGRG 5 cut(s) 314, 1857, 2124, 2352, 2544
Aor13HI TCCGGA 1 cut(s) 1258
Aor51HI AGCGCT 1 cut(s) 1283
ApaI GGGCCC 1 cut(s) 2854
ApeKI GCWGC 5 cut(s) 1298, 1967, 2150, 2245, 2714
ApoI RAATTY 3 cut(s) 536, 550, 594
ArsI GACNNNNNNTTYG 2 cut(s) 1205, 1237
AseI ATTAAT 1 cut(s) 2888
Asp700I GAANNNNTTC 1 cut(s) 17
Asp718I GGTACC 1 cut(s) 1009
AspA2I CCTAGG 1 cut(s) 1364
AspLEI GCGC 5 cut(s) 486, 911, 1284, 1627, 2239
AsuHPI GGTGA 5 cut(s) 155, 269, 1810, 1819, 2129
AvaI CYCGRG 5 cut(s) 314, 1857, 2124, 2352, 2544
AvrII CCTAGG 1 cut(s) 1364
AxyI CCTNAGG 1 cut(s) 1395
BaeGI GKGCMC 2 cut(s) 2041, 2854
BaeI ACNNNNGTAYC 2 cut(s) 614, 647
BalI TGGCCA 2 cut(s) 604, 1879
BanI GGYRCC 6 cut(s) 250, 1009, 1624, 2036, 2236, 2780
BanII GRGCYC 3 cut(s) 963, 2087, 2854
BauI CACGAG 1 cut(s) 174
BbrPI CACGTG 2 cut(s) 1360, 2803
BbsI GAAGAC 1 cut(s) 1398
Bbv12I GWGCWC 1 cut(s) 963
BbvCI CCTCAGC 3 cut(s) 1428, 1567, 1977
BbvI GCAGC 5 cut(s) 1310, 1954, 2137, 2257, 2701
BcgI CGANNNNNNTGC 4 cut(s) 1145, 1179, 2762, 2796
BciT130I CCWGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
BciVI GTATCC 1 cut(s) 1341
BcoDI GTCTC 6 cut(s) 1382, 1641, 1821, 2053, 2057, 2357
BcuI ACTAGT 2 cut(s) 814, 1597
BfaI CTAG 7 cut(s) 447, 572, 815, 825, 1365, 1560, 1598
BfmI CTRYAG 3 cut(s) 1296, 1575, 1647
BfoI RGCGCY 3 cut(s) 1285, 1628, 2240
BfuAI ACCTGC 2 cut(s) 238, 1212
BfuI GTATCC 1 cut(s) 1341
BglI GCCNNNNNGGC 1 cut(s) 2789
BlnI CCTAGG 1 cut(s) 1364
Bme1390I CCNGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
BmeRI GACNNNNNGTC 1 cut(s) 1223
BmeT110I CYCGRG 5 cut(s) 314, 1857, 2124, 2352, 2544
BmrFI CCNGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
BmrI ACTGGG 3 cut(s) 593, 858, 997
BmsI GCATC 5 cut(s) 14, 466, 789, 1616, 1663
BmuI ACTGGG 3 cut(s) 593, 858, 997
BpiI GAAGAC 1 cut(s) 1398
BplI GAGNNNNNCTC 2 cut(s) 1271, 1303
BpmI CTGGAG 1 cut(s) 684
Bpu10I CCTNAGC 4 cut(s) 1428, 1550, 1567, 1977
BpuEI CTTGAG 2 cut(s) 1298, 2505
Bsa29I ATCGAT 2 cut(s) 27, 1462
BsaAI YACGTR 3 cut(s) 1360, 1834, 2803
BsaBI GATNNNNATC 2 cut(s) 1352, 1419
BsaHI GRCGYC 3 cut(s) 1625, 2237, 2485
BsaI GGTCTC 1 cut(s) 1821
BsaWI WCCGGW 2 cut(s) 104, 1258
BsaXI ACNNNNNCTCC 2 cut(s) 1818, 1848
Bse1I ACTGG 6 cut(s) 588, 701, 728, 864, 1003, 1093
Bse21I CCTNAGG 1 cut(s) 1395
Bse3DI GCAATG 2 cut(s) 605, 2784
Bse8I GATNNNNATC 2 cut(s) 1352, 1419
BseAI TCCGGA 1 cut(s) 1258
BseBI CCWGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
BseCI ATCGAT 2 cut(s) 27, 1462
BseGI GGATG 6 cut(s) 5, 219, 358, 2320, 2370, 2586
BseJI GATNNNNATC 2 cut(s) 1352, 1419
BseMI GCAATG 2 cut(s) 605, 2784
BseMII CTCAG 9 cut(s) 39, 1386, 1419, 1541, 1581, 1722, 1968, 2181, 2780
BseNI ACTGG 6 cut(s) 588, 701, 728, 864, 1003, 1093
BseSI GKGCMC 2 cut(s) 2041, 2854
BseX3I CGGCCG 1 cut(s) 2493
BseXI GCAGC 5 cut(s) 1310, 1954, 2137, 2257, 2701
BseYI CCCAGC 2 cut(s) 501, 790
BsgI GTGCAG 1 cut(s) 481
Bsh1285I CGRYCG 2 cut(s) 1462, 2496
BshNI GGYRCC 6 cut(s) 250, 1009, 1624, 2036, 2236, 2780
BshVI ATCGAT 2 cut(s) 27, 1462
BsiEI CGRYCG 2 cut(s) 1462, 2496
BsiHKAI GWGCWC 1 cut(s) 963
BsiHKCI CYCGRG 5 cut(s) 314, 1857, 2124, 2352, 2544
BsiSI CCGG 3 cut(s) 105, 803, 1259
BslFI GGGAC 6 cut(s) 150, 213, 288, 1214, 2008, 2173
BsmAI GTCTC 6 cut(s) 1382, 1641, 1821, 2053, 2057, 2357
BsmBI CGTCTC 1 cut(s) 1641
BsmFI GGGAC 6 cut(s) 150, 213, 288, 1214, 2008, 2173
BsmI GAATGC 5 cut(s) 59, 417, 527, 1515, 2677
Bso31I GGTCTC 1 cut(s) 1821
BsoBI CYCGRG 5 cut(s) 314, 1857, 2124, 2352, 2544
Bsp120I GGGCCC 1 cut(s) 2850
Bsp1286I GDGCHC 4 cut(s) 963, 2041, 2087, 2854
Bsp13I TCCGGA 1 cut(s) 1258
Bsp19I CCATGG 2 cut(s) 589, 2113
Bsp68I TCGCGA 2 cut(s) 678, 2053
BspCNI CTCAG 9 cut(s) 40, 1387, 1420, 1542, 1580, 1723, 1969, 2182, 2779
BspDI ATCGAT 2 cut(s) 27, 1462
BspEI TCCGGA 1 cut(s) 1258
BspHI TCATGA 1 cut(s) 1411
BspMAI CTGCAG 2 cut(s) 1300, 1579
BspMI ACCTGC 2 cut(s) 238, 1212
BspPI GGATC 6 cut(s) 462, 882, 1415, 1557, 1898, 2222
BspQI GCTCTTC 1 cut(s) 1575
BspT107I GGYRCC 6 cut(s) 250, 1009, 1624, 2036, 2236, 2780
BspTNI GGTCTC 1 cut(s) 1821
BsrBI CCGCTC 1 cut(s) 1915
BsrDI GCAATG 2 cut(s) 605, 2784
BsrI ACTGG 6 cut(s) 588, 701, 728, 864, 1003, 1093
BssNI GRCGYC 3 cut(s) 1625, 2237, 2485
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 6 cut(s) 278, 589, 636, 1118, 1364, 2113
Bst2BI CACGAG 1 cut(s) 174
Bst2UI CCWGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
Bst4CI ACNGT 6 cut(s) 1014, 1196, 1874, 1999, 2030, 2866
Bst6I CTCTTC 5 cut(s) 1226, 1473, 1575, 1635, 1821
BstACI GRCGYC 3 cut(s) 1625, 2237, 2485
BstBAI YACGTR 3 cut(s) 1360, 1834, 2803
BstC8I GCNNGC 3 cut(s) 985, 1450, 2594
BstDSI CCRYGG 4 cut(s) 589, 1013, 1620, 2113
BstENI CCTNNNNNAGG 1 cut(s) 1553
BstF5I GGATG 6 cut(s) 5, 219, 358, 2320, 2370, 2586
BstH2I RGCGCY 3 cut(s) 1285, 1628, 2240
BstHHI GCGC 5 cut(s) 486, 911, 1284, 1627, 2239
BstMAI GTCTC 6 cut(s) 1382, 1641, 1821, 2053, 2057, 2357
BstMCI CGRYCG 2 cut(s) 1462, 2496
BstNI CCWGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
BstNSI RCATGY 1 cut(s) 2884
BstSCI CCNGG 9 cut(s) 253, 531, 1069, 1252, 1342, 1938, 2409, 2505, 2739
BstSFI CTRYAG 3 cut(s) 1296, 1575, 1647
BstSLI GKGCMC 2 cut(s) 2041, 2854
BstV1I GCAGC 5 cut(s) 1310, 1954, 2137, 2257, 2701
BstV2I GAAGAC 1 cut(s) 1398
BstX2I RGATCY 3 cut(s) 467, 1420, 1562
BstYI RGATCY 3 cut(s) 467, 1420, 1562
BstZI CGGCCG 1 cut(s) 2493
Bsu15I ATCGAT 2 cut(s) 27, 1462
Bsu36I CCTNAGG 1 cut(s) 1395
BsuI GTATCC 1 cut(s) 1341
BsuTUI ATCGAT 2 cut(s) 27, 1462
BtgI CCRYGG 4 cut(s) 589, 1013, 1620, 2113
BtgZI GCGATG 3 cut(s) 292, 693, 1320
BtsCI GGATG 6 cut(s) 5, 219, 358, 2320, 2370, 2586
BtsI GCAGTG 2 cut(s) 1434, 1572
BtsIMutI CAGTG 5 cut(s) 45, 871, 1434, 1572, 1870
BtuMI TCGCGA 2 cut(s) 678, 2053
BveI ACCTGC 2 cut(s) 238, 1212
Cac8I GCNNGC 3 cut(s) 985, 1450, 2594
CaiI CAGNNNCTG 1 cut(s) 1574
CciI TCATGA 1 cut(s) 1411
CfoI GCGC 5 cut(s) 486, 911, 1284, 1627, 2239
ClaI ATCGAT 2 cut(s) 27, 1462
CseI GACGC 2 cut(s) 1328, 2525
Csp6I GTAC 6 cut(s) 623, 784, 1010, 1135, 1835, 2330
CspCI CAANNNNNGTGG 4 cut(s) 965, 1000, 2828, 2863
CviQI GTAC 6 cut(s) 623, 784, 1010, 1135, 1835, 2330
DinI GGCGCC 2 cut(s) 1626, 2238
DraI TTTAAA 1 cut(s) 2574
DriI GACNNNNNGTC 1 cut(s) 1223
EaeI YGGCCR 3 cut(s) 602, 1877, 2493
EagI CGGCCG 1 cut(s) 2493
Eam1104I CTCTTC 5 cut(s) 1226, 1473, 1575, 1635, 1821
Eam1105I GACNNNNNGTC 1 cut(s) 1223
EarI CTCTTC 5 cut(s) 1226, 1473, 1575, 1635, 1821
EciI GGCGGA 2 cut(s) 85, 2579
Ecl136II GAGCTC 1 cut(s) 961
EclXI CGGCCG 1 cut(s) 2493
Eco130I CCWWGG 6 cut(s) 278, 589, 636, 1118, 1364, 2113
Eco147I AGGCCT 1 cut(s) 1813
Eco24I GRGCYC 3 cut(s) 963, 2087, 2854
Eco31I GGTCTC 1 cut(s) 1821
Eco32I GATATC 1 cut(s) 1150
Eco47III AGCGCT 1 cut(s) 1283
Eco52I CGGCCG 1 cut(s) 2493
Eco53kI GAGCTC 1 cut(s) 961
Eco57I CTGAAG 3 cut(s) 2175, 2615, 2742
Eco72I CACGTG 2 cut(s) 1360, 2803
Eco81I CCTNAGG 1 cut(s) 1395
Eco88I CYCGRG 5 cut(s) 314, 1857, 2124, 2352, 2544
EcoICRI GAGCTC 1 cut(s) 961
EcoNI CCTNNNNNAGG 1 cut(s) 1553
EcoO109I RGGNCCY 8 cut(s) 164, 1201, 1664, 1753, 1982, 2348, 2641, 2851
EcoRI GAATTC 2 cut(s) 536, 594
EcoRII CCWGG 9 cut(s) 253, 531, 1069, 1252, 1342, 1938, 2409, 2505, 2739
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 6 cut(s) 278, 589, 636, 1118, 1364, 2113
EcoT38I GRGCYC 3 cut(s) 963, 2087, 2854
EgeI GGCGCC 2 cut(s) 1626, 2238
EheI GGCGCC 2 cut(s) 1626, 2238
ErhI CCWWGG 6 cut(s) 278, 589, 636, 1118, 1364, 2113
Esp3I CGTCTC 1 cut(s) 1641
FaqI GGGAC 6 cut(s) 150, 213, 288, 1214, 2008, 2173
FauI CCCGC 7 cut(s) 1317, 1432, 1920, 1985, 2385, 2570, 2589
FblI GTMKAC 1 cut(s) 121
FokI GGATG 5 cut(s) 206, 365, 2327, 2357, 2573
FriOI GRGCYC 3 cut(s) 963, 2087, 2854
FspBI CTAG 7 cut(s) 447, 572, 815, 825, 1365, 1560, 1598
GlaI GCGC 5 cut(s) 485, 910, 1283, 1626, 2238
GsaI CCCAGC 2 cut(s) 505, 794
GsuI CTGGAG 1 cut(s) 684
HaeII RGCGCY 3 cut(s) 1285, 1628, 2240
HapII CCGG 3 cut(s) 105, 803, 1259
HgaI GACGC 2 cut(s) 1328, 2525
HhaI GCGC 5 cut(s) 486, 911, 1284, 1627, 2239
Hin1I GRCGYC 3 cut(s) 1625, 2237, 2485
Hin6I GCGC 5 cut(s) 484, 909, 1282, 1625, 2237
HinP1I GCGC 5 cut(s) 484, 909, 1282, 1625, 2237
HincII GTYRAC 2 cut(s) 122, 382
HindII GTYRAC 2 cut(s) 122, 382
HpaII CCGG 3 cut(s) 105, 803, 1259
HphI GGTGA 5 cut(s) 155, 269, 1810, 1819, 2129
Hpy166II GTNNAC 6 cut(s) 122, 382, 869, 1098, 1192, 2203
Hpy8I GTNNAC 6 cut(s) 122, 382, 869, 1098, 1192, 2203
Hpy99I CGWCG 7 cut(s) 678, 909, 1157, 1478, 1768, 2487, 2519
HpyAV CCTTC 6 cut(s) 679, 1335, 2150, 2313, 2410, 2904
HpyCH4III ACNGT 6 cut(s) 1014, 1196, 1874, 1999, 2030, 2866
HpyCH4IV ACGT 5 cut(s) 738, 1359, 1833, 2485, 2802
HpySE526I ACGT 5 cut(s) 738, 1359, 1833, 2485, 2802
Hsp92I GRCGYC 3 cut(s) 1625, 2237, 2485
HspAI GCGC 5 cut(s) 484, 909, 1282, 1625, 2237
KasI GGCGCC 2 cut(s) 1624, 2236
KflI GGGWCCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 1258
KpnI GGTACC 1 cut(s) 1013
LguI GCTCTTC 1 cut(s) 1575
LmnI GCTCC 4 cut(s) 757, 1705, 2075, 2107
Lsp1109I GCAGC 5 cut(s) 1310, 1954, 2137, 2257, 2701
LweI GCATC 5 cut(s) 14, 466, 789, 1616, 1663
MaeI CTAG 7 cut(s) 447, 572, 815, 825, 1365, 1560, 1598
MaeII ACGT 5 cut(s) 738, 1359, 1833, 2485, 2802
MaeIII GTNAC 3 cut(s) 506, 2293, 2761
MbiI CCGCTC 1 cut(s) 1915
MflI RGATCY 3 cut(s) 467, 1420, 1562
MhlI GDGCHC 4 cut(s) 963, 2041, 2087, 2854
MlsI TGGCCA 2 cut(s) 604, 1879
MluCI AATT 9 cut(s) 422, 536, 550, 594, 989, 1036, 1469, 2435, 2822
MluNI TGGCCA 2 cut(s) 604, 1879
Mly113I GGCGCC 2 cut(s) 1625, 2237
MlyI GAGTC 7 cut(s) 166, 1212, 1384, 1412, 1691, 2391, 2444
MmeI TCCRAC 3 cut(s) 1314, 1931, 2268
Mox20I TGGCCA 2 cut(s) 604, 1879
MroI TCCGGA 1 cut(s) 1258
MroXI GAANNNNTTC 1 cut(s) 17
MscI TGGCCA 2 cut(s) 604, 1879
MseI TTAA 4 cut(s) 431, 2573, 2888, 2901
MslI CAYNNNNRTG 2 cut(s) 64, 344
Msp20I TGGCCA 2 cut(s) 604, 1879
MspA1I CMGCKG 1 cut(s) 1427
MspI CCGG 3 cut(s) 105, 803, 1259
MspR9I CCNGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
Mva1269I GAATGC 5 cut(s) 59, 417, 527, 1515, 2677
MvaI CCWGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
NarI GGCGCC 2 cut(s) 1625, 2237
NcoI CCATGG 2 cut(s) 589, 2113
NmeAIII GCCGAG 1 cut(s) 2104
NmuCI GTSAC 2 cut(s) 506, 2761
NruI TCGCGA 2 cut(s) 678, 2053
NspI RCATGY 1 cut(s) 2884
PaeR7I CTCGAG 4 cut(s) 314, 1857, 2124, 2544
PagI TCATGA 1 cut(s) 1411
PaqCI CACCTGC 1 cut(s) 238
PceI AGGCCT 1 cut(s) 1813
PciSI GCTCTTC 1 cut(s) 1575
PcsI WCGNNNNNNNCGW 3 cut(s) 1152, 1221, 1336
PctI GAATGC 5 cut(s) 59, 417, 527, 1515, 2677
PdmI GAANNNNTTC 1 cut(s) 17
PfoI TCCNGGA 1 cut(s) 531
Ple19I CGATCG 1 cut(s) 1462
PleI GAGTC 7 cut(s) 165, 1212, 1384, 1411, 1691, 2390, 2444
PluTI GGCGCC 2 cut(s) 1628, 2240
PmaCI CACGTG 2 cut(s) 1360, 2803
PmlI CACGTG 2 cut(s) 1360, 2803
PpsI GAGTC 7 cut(s) 165, 1212, 1384, 1411, 1691, 2390, 2444
Ppu21I YACGTR 3 cut(s) 1360, 1834, 2803
PpuMI RGGWCCY 7 cut(s) 164, 1201, 1664, 1753, 1982, 2348, 2641
PshBI ATTAAT 1 cut(s) 2888
Psp124BI GAGCTC 1 cut(s) 963
Psp5II RGGWCCY 7 cut(s) 164, 1201, 1664, 1753, 1982, 2348, 2641
Psp6I CCWGG 9 cut(s) 253, 531, 1069, 1252, 1342, 1938, 2409, 2505, 2739
PspCI CACGTG 2 cut(s) 1360, 2803
PspFI CCCAGC 2 cut(s) 501, 790
PspGI CCWGG 9 cut(s) 253, 531, 1069, 1252, 1342, 1938, 2409, 2505, 2739
PspOMI GGGCCC 1 cut(s) 2850
PspPPI RGGWCCY 7 cut(s) 164, 1201, 1664, 1753, 1982, 2348, 2641
PspXI VCTCGAGB 1 cut(s) 2124
PstI CTGCAG 2 cut(s) 1300, 1579
PstNI CAGNNNCTG 1 cut(s) 1574
PsuI RGATCY 3 cut(s) 467, 1420, 1562
PvuI CGATCG 1 cut(s) 1462
RruI TCGCGA 2 cut(s) 678, 2053
RsaI GTAC 6 cut(s) 624, 785, 1011, 1136, 1836, 2331
RsaNI GTAC 6 cut(s) 623, 784, 1010, 1135, 1835, 2330
RseI CAYNNNNRTG 2 cut(s) 64, 344
SacI GAGCTC 1 cut(s) 963
SalI GTCGAC 1 cut(s) 120
SapI GCTCTTC 1 cut(s) 1575
SaqAI TTAA 4 cut(s) 431, 2573, 2888, 2901
SchI GAGTC 7 cut(s) 166, 1212, 1384, 1412, 1691, 2391, 2444
ScrFI CCNGG 9 cut(s) 255, 533, 1071, 1254, 1344, 1940, 2411, 2507, 2741
SduI GDGCHC 4 cut(s) 963, 2041, 2087, 2854
SfaNI GCATC 5 cut(s) 14, 466, 789, 1616, 1663
SfcI CTRYAG 3 cut(s) 1296, 1575, 1647
SfoI GGCGCC 2 cut(s) 1626, 2238
Sfr274I CTCGAG 4 cut(s) 314, 1857, 2124, 2544
SlaI CTCGAG 4 cut(s) 314, 1857, 2124, 2544
SmiMI CAYNNNNRTG 2 cut(s) 64, 344
SmlI CTYRAG 6 cut(s) 314, 1277, 1857, 2124, 2520, 2544
SmoI CTYRAG 6 cut(s) 314, 1277, 1857, 2124, 2520, 2544
SpeI ACTAGT 2 cut(s) 814, 1597
Sse9I AATT 9 cut(s) 422, 536, 550, 594, 989, 1036, 1469, 2435, 2822
SseBI AGGCCT 1 cut(s) 1813
SspDI GGCGCC 2 cut(s) 1624, 2236
SspMI CTAG 7 cut(s) 447, 572, 815, 825, 1365, 1560, 1598
SstI GAGCTC 1 cut(s) 963
StuI AGGCCT 1 cut(s) 1813
StyD4I CCNGG 9 cut(s) 253, 531, 1069, 1252, 1342, 1938, 2409, 2505, 2739
StyI CCWWGG 6 cut(s) 278, 589, 636, 1118, 1364, 2113
TaaI ACNGT 6 cut(s) 1014, 1196, 1874, 1999, 2030, 2866
TaiI ACGT 5 cut(s) 741, 1362, 1836, 2488, 2805
TasI AATT 9 cut(s) 422, 536, 550, 594, 989, 1036, 1469, 2435, 2822
TatI WGTACW 1 cut(s) 783
TauI GCSGC 8 cut(s) 59, 239, 735, 1745, 1781, 2498, 2736, 2792
Tru1I TTAA 4 cut(s) 431, 2573, 2888, 2901
Tru9I TTAA 4 cut(s) 431, 2573, 2888, 2901
TscAI CASTG 5 cut(s) 52, 871, 1441, 1579, 1877
TseFI GTSAC 2 cut(s) 506, 2761
TseI GCWGC 5 cut(s) 1298, 1967, 2150, 2245, 2714
Tsp45I GTSAC 2 cut(s) 506, 2761
TspDTI ATGAA 7 cut(s) 54, 1431, 1725, 2196, 2478, 2592, 2718
TspGWI ACGGA 7 cut(s) 215, 662, 1177, 1230, 1511, 1623, 1781
TspRI CASTG 5 cut(s) 52, 871, 1441, 1579, 1877
VspI ATTAAT 1 cut(s) 2888
XagI CCTNNNNNAGG 1 cut(s) 1553
XapI RAATTY 3 cut(s) 536, 550, 594
XceI RCATGY 1 cut(s) 2884
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XhoI CTCGAG 4 cut(s) 314, 1857, 2124, 2544
XmaJI CCTAGG 1 cut(s) 1364
XmiI GTMKAC 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 7 cut(s) 447, 572, 815, 825, 1365, 1560, 1598
ZraI GACGTC 1 cut(s) 2486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.