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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 114 |
| AciI | CCGC | 3 cut(s) | 55, 112, 223 |
| AclWI | GGATC | 1 cut(s) | 138 |
| AcuI | CTGAAG | 1 cut(s) | 231 |
| AcvI | CACGTG | 1 cut(s) | 292 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 295 |
| AgsI | TTSAA | 3 cut(s) | 34, 122, 310 |
| AluBI | AGCT | 3 cut(s) | 182, 266, 318 |
| AluI | AGCT | 3 cut(s) | 182, 266, 318 |
| Alw26I | GTCTC | 1 cut(s) | 91 |
| AlwI | GGATC | 1 cut(s) | 138 |
| AoxI | GGCC | 5 cut(s) | 81, 220, 249, 258, 325 |
| ApaI | GGGCCC | 1 cut(s) | 85 |
| ApeKI | GCWGC | 3 cut(s) | 4, 68, 203 |
| AspS9I | GGNCC | 4 cut(s) | 81, 82, 220, 249 |
| AxyI | CCTNAGG | 1 cut(s) | 86 |
| BaeGI | GKGCMC | 1 cut(s) | 85 |
| BanII | GRGCYC | 2 cut(s) | 52, 85 |
| BbrPI | CACGTG | 1 cut(s) | 292 |
| BbvI | GCAGC | 3 cut(s) | 16, 80, 190 |
| BccI | CCATC | 1 cut(s) | 66 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 251, 285 |
| BcoDI | GTCTC | 1 cut(s) | 91 |
| BfmI | CTRYAG | 1 cut(s) | 2 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 257 |
| BisI | GCNGC | 5 cut(s) | 5, 55, 69, 204, 223 |
| BlsI | GCNGC | 5 cut(s) | 6, 56, 70, 205, 224 |
| BmgT120I | GGNCC | 4 cut(s) | 81, 82, 220, 249 |
| BmiI | GGNNCC | 1 cut(s) | 83 |
| BmrI | ACTGGG | 1 cut(s) | 88 |
| BmuI | ACTGGG | 1 cut(s) | 88 |
| BsaAI | YACGTR | 1 cut(s) | 292 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 295 |
| Bse1I | ACTGG | 1 cut(s) | 83 |
| Bse21I | CCTNAGG | 1 cut(s) | 86 |
| BseGI | GGATG | 1 cut(s) | 104 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 295 |
| BseMII | CTCAG | 2 cut(s) | 100, 269 |
| BseNI | ACTGG | 1 cut(s) | 83 |
| BseSI | GKGCMC | 1 cut(s) | 85 |
| BseXI | GCAGC | 3 cut(s) | 16, 80, 190 |
| Bsh1236I | CGCG | 1 cut(s) | 114 |
| BshFI | GGCC | 5 cut(s) | 83, 222, 251, 260, 327 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 295 |
| BsmAI | GTCTC | 1 cut(s) | 91 |
| BsmI | GAATGC | 1 cut(s) | 166 |
| BsnI | GGCC | 5 cut(s) | 83, 222, 251, 260, 327 |
| Bsp120I | GGGCCC | 1 cut(s) | 81 |
| Bsp1286I | GDGCHC | 2 cut(s) | 52, 85 |
| Bsp143I | GATC | 3 cut(s) | 25, 130, 244 |
| BspACI | CCGC | 3 cut(s) | 55, 112, 223 |
| BspANI | GGCC | 5 cut(s) | 83, 222, 251, 260, 327 |
| BspCNI | CTCAG | 2 cut(s) | 99, 268 |
| BspFNI | CGCG | 1 cut(s) | 114 |
| BspLI | GGNNCC | 1 cut(s) | 83 |
| BspMAI | CTGCAG | 1 cut(s) | 6 |
| BspPI | GGATC | 1 cut(s) | 138 |
| BsrI | ACTGG | 1 cut(s) | 83 |
| BssMI | GATC | 3 cut(s) | 25, 130, 244 |
| BstBAI | YACGTR | 1 cut(s) | 292 |
| BstC8I | GCNNGC | 2 cut(s) | 52, 325 |
| BstDEI | CTNAG | 2 cut(s) | 86, 255 |
| BstF5I | GGATG | 1 cut(s) | 104 |
| BstFNI | CGCG | 1 cut(s) | 114 |
| BstKTI | GATC | 3 cut(s) | 28, 133, 247 |
| BstMAI | GTCTC | 1 cut(s) | 91 |
| BstMBI | GATC | 3 cut(s) | 25, 130, 244 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 146, 170, 179, 257, 278, 324 |
| BstSFI | CTRYAG | 1 cut(s) | 2 |
| BstSLI | GKGCMC | 1 cut(s) | 85 |
| BstUI | CGCG | 1 cut(s) | 114 |
| BstV1I | GCAGC | 3 cut(s) | 16, 80, 190 |
| BstX2I | RGATCY | 1 cut(s) | 130 |
| BstYI | RGATCY | 1 cut(s) | 130 |
| Bsu36I | CCTNAGG | 1 cut(s) | 86 |
| BsuRI | GGCC | 5 cut(s) | 83, 222, 251, 260, 327 |
| BtsCI | GGATG | 1 cut(s) | 104 |
| Cac8I | GCNNGC | 2 cut(s) | 52, 325 |
| Cfr13I | GGNCC | 4 cut(s) | 81, 82, 220, 249 |
| DdeI | CTNAG | 2 cut(s) | 86, 255 |
| DpnI | GATC | 3 cut(s) | 27, 132, 246 |
| DpnII | GATC | 3 cut(s) | 25, 130, 244 |
| Eco24I | GRGCYC | 2 cut(s) | 52, 85 |
| Eco57I | CTGAAG | 1 cut(s) | 231 |
| Eco72I | CACGTG | 1 cut(s) | 292 |
| Eco81I | CCTNAGG | 1 cut(s) | 86 |
| EcoO109I | RGGNCCY | 1 cut(s) | 82 |
| EcoT38I | GRGCYC | 2 cut(s) | 52, 85 |
| FauI | CCCGC | 1 cut(s) | 119 |
| Fnu4HI | GCNGC | 5 cut(s) | 5, 55, 69, 204, 223 |
| FokI | GGATG | 1 cut(s) | 91 |
| FriOI | GRGCYC | 2 cut(s) | 52, 85 |
| Fsp4HI | GCNGC | 5 cut(s) | 5, 55, 69, 204, 223 |
| GluI | GCNGC | 5 cut(s) | 5, 55, 69, 204, 223 |
| HaeIII | GGCC | 5 cut(s) | 83, 222, 251, 260, 327 |
| Hpy188I | TCNGA | 2 cut(s) | 211, 242 |
| HpyCH4IV | ACGT | 1 cut(s) | 291 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 173, 323 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 146, 170, 179, 257, 278, 324 |
| HpyF3I | CTNAG | 2 cut(s) | 86, 255 |
| HpySE526I | ACGT | 1 cut(s) | 291 |
| Kzo9I | GATC | 3 cut(s) | 25, 130, 244 |
| LpnPI | CCDG | 6 cut(s) | 64, 73, 242, 242, 252, 309 |
| Lsp1109I | GCAGC | 3 cut(s) | 16, 80, 190 |
| MaeII | ACGT | 1 cut(s) | 291 |
| MalI | GATC | 3 cut(s) | 27, 132, 246 |
| MboI | GATC | 3 cut(s) | 25, 130, 244 |
| MboII | GAAGA | 2 cut(s) | 188, 224 |
| MflI | RGATCY | 1 cut(s) | 130 |
| MhlI | GDGCHC | 2 cut(s) | 52, 85 |
| MluCI | AATT | 2 cut(s) | 8, 311 |
| MnlI | CCTC | 6 cut(s) | 95, 118, 121, 217, 271, 289 |
| Mva1269I | GAATGC | 1 cut(s) | 166 |
| MvnI | CGCG | 1 cut(s) | 114 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 146, 170, 179, 257, 278, 324 |
| NdeII | GATC | 3 cut(s) | 25, 130, 244 |
| NlaIV | GGNNCC | 1 cut(s) | 83 |
| PctI | GAATGC | 1 cut(s) | 166 |
| PkrI | GCNGC | 5 cut(s) | 6, 56, 70, 205, 224 |
| PmaCI | CACGTG | 1 cut(s) | 292 |
| PmlI | CACGTG | 1 cut(s) | 292 |
| Ppu21I | YACGTR | 1 cut(s) | 292 |
| PspCI | CACGTG | 1 cut(s) | 292 |
| PspN4I | GGNNCC | 1 cut(s) | 83 |
| PspOMI | GGGCCC | 1 cut(s) | 81 |
| PspPI | GGNCC | 4 cut(s) | 81, 82, 220, 249 |
| PstI | CTGCAG | 1 cut(s) | 6 |
| PsuI | RGATCY | 1 cut(s) | 130 |
| SatI | GCNGC | 5 cut(s) | 5, 55, 69, 204, 223 |
| Sau3AI | GATC | 3 cut(s) | 25, 130, 244 |
| Sau96I | GGNCC | 4 cut(s) | 81, 82, 220, 249 |
| SduI | GDGCHC | 2 cut(s) | 52, 85 |
| SetI | ASST | 7 cut(s) | 92, 184, 199, 231, 268, 294, 320 |
| SfcI | CTRYAG | 1 cut(s) | 2 |
| SfiI | GGCCNNNNNGGCC | 1 cut(s) | 257 |
| Sse9I | AATT | 2 cut(s) | 8, 311 |
| SsiI | CCGC | 3 cut(s) | 55, 112, 223 |
| TaiI | ACGT | 1 cut(s) | 294 |
| TaqI | TCGA | 2 cut(s) | 126, 189 |
| TasI | AATT | 2 cut(s) | 8, 311 |
| TauI | GCSGC | 2 cut(s) | 57, 225 |
| TseI | GCWGC | 3 cut(s) | 4, 68, 203 |
| TspDTI | ATGAA | 2 cut(s) | 51, 207 |