Rorug07G0321500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000195
Physical Location & Seq
Reverse (-)
33047893 .. 33058774
10882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0321500.1

Sequence Viewer

Length: 2289 bp
ATGGGTTCCAAGAAGAAGGGAAAGTTTGAAAGAAAGAAAAAATTAGTGGAAGAAGCGGATGTGAAGACTTGTCCCGAGTCTGATGAGTCTGTTGAGAAGCAAGTAGAATCTATATCAGCAGACACCGTCACAACCAGCGAATCAACTAAAATTAGAGGGAAGCGAAGTGTGGTGGAAATGTACAATGTTTTGATCAAGAAAGCATTGGGTAAAAAGTTGAAAGTGACATACACTGACACTGGCAATCCAAATGGACGAGCACGGCACAATCTGCAATCATACATAGGAATGCTGGCGCGTAAAATGGTGCCGATCAACATTGAAAGCTGGCCTGCAGTGGACAAAGACTTGAAAGATAAAATTTGGATTGATGTCCAGGATACATTCAAAGTGGCCCCTGAAAGCAAGAAATTGGTGTTGACATCTGCCGGCACTAAATGGAGACAATTCAAGACCTACTTAACAAATAAGCATGTGCTGCCATACTTGGGGAAGAAGAAGAAACTGAGGAAGCCACCGAAGCAATATGCATTCGTTGGGTTACAACCATGGAAGGAATTTGTAACTCAGAGGAAGACAGAGGAATGGCTGAAACTTAACAATGACCAAAGGGAACGAGTCAAGAAGAGAAAATATCATCATAGATTATCAAGAAAGGGATATATTGGACTGGAGGAAGAATTGAGAGATACTTGGCCTGAAGGAGAGGTAATTGATCGCGCTATTATGTGGAAGAAAGCCCGTTTACTAAAAAATGGGGAGATAAGTGAAGAGGTCACACCGGTAGCAAGAAAGATAGATGAATTGTTGGAGAAGAAGAGTAAGGGTGAGCTAGAAATATCAGGGAGCGATGATGTTCTCACTCAAGCACTAGAAACTCCTGAACACTCGGGTAGGGTGAGGGGTGTTGGAGGCTTCGTCAATCCGTCCACCTACTTTAAGCTTCCAAAGCAGAAAAGGGTCAGAATTACCAAGGCAGAATTAATGGCACGTGATAGAGAGCGTAATCGGGAGTTGGAGGAGACAAAGAAAATGCTTATTGAAAAACAAGCAAAGGCAGAAGATCTCTTAAACAAAAGGATTGCTCAACTAGAAGCACTAATAACAGACAAGGCGAACTATGCACCCCATTCCCCTATATCAGATAAGGGCAGTTTTCATGATAGAGTGAAAAAAAATTCCCCTATATTAGATGACAAGGTGCAAGAAAATGTGGATGATTGTGAAATTGTTCCTCCACCTACTGATAAGGGTGGCGTATGTGAGTTGGCAGTGAATAACATCACCAACATAGTTGCTTTTGGTACCGTATTCGATGATGTGGATATTAATAAGATGATACATGGAGCTGCATTGAAGGAGGGATGCGTTAGAGTGTCAGTGGATGGTGAGATTCAAGGTGATGCACCACTTCCATTCCCTATAGCGGGTGAGATGGAATTGGTTCGAGAAGCCATTGGAAGTCACGTGGCTTGGCCTGAGGAGCTTGTAATTCGGAGACAGCCATCAAAGAAAAAGATAAGGAAGAATGATTTTGTCAAATCATTGTTCAATAAAGCTGAGGTCAACCCGTTTGTGCCCAAGCGCTGCAAGCTATTGTACAAACATGCCACAACAATCATGACACATACTAGTGAGGCAATATCCACAGTATTGGACGATAAAGTCTTTGGCGTACACAAAGAACTCTTCATCTTGACAGAGAATGTGATTGATCTGTTAGAAATGCAGAAGATTGGCCAAGGAGTGATAGCAGCATACATGGCCCACCTACATGAAGTTGTTACTGAAAGAGACGAGTTTGATAATTTTGCCTTTATTGATCCTGCTGCTACATATAACTGTGAGAGATCAGATTTTGGTTCCTATTTAGTGAATCGGTTGAAAGAGGGCAAGACTGATCGCATATTCTTTATGCCCTACAATCCAGGGGAACATTGGATATTGACAATCATATGGGAGGATGATATCTACATCTTGGACCCTTTGGGGAAATCAGTCCATTACCAAGCATGGGAGAACTCGGTGATAAATGCGGTTAAAAGTTTCAATGCTGAAACGGGTAGGGCTAACAAGGTGCCGAAATTGAAACTACTTCCGGGCGTACCTAAACAACCGGGTGGAATTGAATGTGGCTATTATGTAATGAGATATATGAAGGAGATTATAAATGATGACACATTTTCGTTTCCGACTAAGTGGGCAGCAAAGACTCGGAAAGGATACACCCAACAACAACTTGACGAGGTGCGCATTGAGGTGGCTGACTATTTGCAGACTTTGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

762

Amino Acids

87.13

Weight (kDa)

9.13

Isoelectric Point (pI)

36.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 184 - 309 7.3e-07 Plant transposase (Ptta/En/Spm family)
DUF8039 PF26133 421 - 498 4.5e-12 Domain of unknown function (DUF8039)
Peptidase_C48 PF02902 636 - 750 1.4e-06 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000195)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g25780 FvH4_6g25920
rosa_chinensis RchiOBHm_Chr1g0366971 RchiOBHm_Chr1g0372841 RchiOBHm_Chr2g0146841 RchiOBHm_Chr2g0154821 RchiOBHm_Chr2g0159611 RchiOBHm_Chr3g0452071 RchiOBHm_Chr3g0453731 RchiOBHm_Chr3g0457891 RchiOBHm_Chr3g0471041 RchiOBHm_Chr3g0473741 RchiOBHm_Chr4g0427761 RchiOBHm_Chr5g0041301 RchiOBHm_Chr5g0076511 RchiOBHm_Chr6g0281431 RchiOBHm_Chr7g0180511
rosa_multiflora Rmu_sc0000082.1_g000052 Rmu_sc0000125.1_g000058 Rmu_sc0000147.1_g000108 Rmu_sc0000210.1_g000011 Rmu_sc0000229.1_g000006 Rmu_sc0000245.1_g000013 Rmu_sc0000897.1_g000016 Rmu_sc0001059.1_g000005 Rmu_sc0001336.1_g000059 Rmu_sc0001464.1_g000025 Rmu_sc0001630.1_g000013 Rmu_sc0001790.1_g000012 Rmu_sc0001939.1_g000003 Rmu_sc0002132.1_g000013 Rmu_sc0002235.1_g000008 Rmu_sc0002235.1_g000022 Rmu_sc0002548.1_g000030 Rmu_sc0002564.1_g000018 Rmu_sc0002652.1_g000001 Rmu_sc0002759.1_g000007 Rmu_sc0002759.1_g000011 Rmu_sc0002799.1_g000012 Rmu_sc0002858.1_g000033 Rmu_sc0002882.1_g000041 Rmu_sc0003032.1_g000030 Rmu_sc0003033.1_g000041 Rmu_sc0003044.1_g000038 Rmu_sc0003072.1_g000004 Rmu_sc0003264.1_g000025 Rmu_sc0003304.1_g000034 Rmu_sc0003416.1_g000002 Rmu_sc0003613.1_g000010 Rmu_sc0003762.1_g000009 Rmu_sc0003776.1_g000028 Rmu_sc0003859.1_g000025 Rmu_sc0004553.1_g000014 Rmu_sc0004866.1_g000002 Rmu_sc0004941.1_g000047 Rmu_sc0005777.1_g000002 Rmu_sc0005958.1_g000001 Rmu_sc0006573.1_g000001 Rmu_sc0006912.1_g000005 Rmu_sc0006968.1_g000002 Rmu_sc0006986.1_g000020 Rmu_sc0007258.1_g000016 Rmu_sc0007803.1_g000001 Rmu_sc0008479.1_g000003 Rmu_sc0008506.1_g000012 Rmu_sc0009199.1_g000005 Rmu_sc0009431.1_g000008 Rmu_sc0009611.1_g000005 Rmu_sc0014287.1_g000001 Rmu_sc0014768.1_g000001 Rmu_sc0020577.1_g000001 Rmu_sc0028131.1_g000001 Rmu_sc0029371.1_g000001 Rmu_sc0034219.1_g000001 Rmu_ssc0000252.1_g000026 Rmu_ssc0000252.1_g000027 Rmu_ssc0000398.1_g000023 Rmu_ssc0000482.1_g000020
rosa_roxburghii Rroxscaffold_158G00443700 Rroxscaffold_1G00001910 Rroxscaffold_1G00001960 Rroxscaffold_1G00002400 Rroxscaffold_1G00015110 Rroxscaffold_1G00015140 Rroxscaffold_1G00028870 Rroxscaffold_1G00028930 Rroxscaffold_1G00031320 Rroxscaffold_1G00031990 Rroxscaffold_1G00060310 Rroxscaffold_1G00063330 Rroxscaffold_1G00071570 Rroxscaffold_2G00084360 Rroxscaffold_2G00084370 Rroxscaffold_2G00088550 Rroxscaffold_2G00088560 Rroxscaffold_2G00089850 Rroxscaffold_2G00095470 Rroxscaffold_2G00103510 Rroxscaffold_2G00114380 Rroxscaffold_2G00119580 Rroxscaffold_2G00122990 Rroxscaffold_2G00125720 Rroxscaffold_3G00223380 Rroxscaffold_3G00230410 Rroxscaffold_3G00233640 Rroxscaffold_3G00234580 Rroxscaffold_3G00241070 Rroxscaffold_4G00282590 Rroxscaffold_4G00309420 Rroxscaffold_4G00309430 Rroxscaffold_4G00314030 Rroxscaffold_4G00314610 Rroxscaffold_4G00316420 Rroxscaffold_4G00319130 Rroxscaffold_4G00319140 Rroxscaffold_4G00319350 Rroxscaffold_4G00324170 Rroxscaffold_4G00324230 Rroxscaffold_5G00346540 Rroxscaffold_5G00346580 Rroxscaffold_5G00346790 Rroxscaffold_5G00349270 Rroxscaffold_5G00350530 Rroxscaffold_5G00373500 Rroxscaffold_5G00378950 Rroxscaffold_5G00378960 Rroxscaffold_5G00381870 Rroxscaffold_6G00388230 Rroxscaffold_6G00389700 Rroxscaffold_6G00389810 Rroxscaffold_6G00391690 Rroxscaffold_6G00391820 Rroxscaffold_6G00391830 Rroxscaffold_6G00393500 Rroxscaffold_6G00396180 Rroxscaffold_6G00396460 Rroxscaffold_6G00418900 Rroxscaffold_6G00423330 Rroxscaffold_7G00173340 Rroxscaffold_7G00176630 Rroxscaffold_7G00181910 Rroxscaffold_7G00191850 Rroxscaffold_7G00199230 Rroxscaffold_7G00199250 Rroxscaffold_7G00203720 Rroxscaffold_7G00204410 Rroxscaffold_7G00204420 Rroxscaffold_7G00212090 Rroxscaffold_7G00215450
rosa_rugosa Rorug07G0321500
rosa_samantha Rh3BG349100 Rh4AG240700
rosa_wichuraiana Rw0G011180 Rw1G003000 Rw5G028290 Rw6G011280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2168
AasI GACNNNNNNGTC 1 cut(s) 1664
Acc16I TGCGCA 1 cut(s) 2252
Acc65I GGTACC 1 cut(s) 1304
AccB1I GGYRCC 3 cut(s) 307, 1304, 2077
AccII CGCG 2 cut(s) 298, 720
AciI CCGC 3 cut(s) 56, 1427, 2036
AclWI GGATC 1 cut(s) 1817
AcoI YGGCCR 1 cut(s) 1738
AcsI RAATTY 3 cut(s) 360, 557, 1177
AcuI CTGAAG 1 cut(s) 720
AcvI CACGTG 2 cut(s) 992, 1468
AfaI GTAC 5 cut(s) 182, 1306, 1601, 1677, 2106
AfeI AGCGCT 1 cut(s) 1586
AfiI CCNNNNNNNGG 4 cut(s) 488, 1426, 1427, 2014
AgeI ACCGGT 1 cut(s) 781
AhlI ACTAGT 1 cut(s) 1631
AjnI CCWGG 2 cut(s) 375, 1927
AluBI AGCT 7 cut(s) 327, 832, 943, 1349, 1486, 1559, 1594
AluI AGCT 7 cut(s) 327, 832, 943, 1349, 1486, 1559, 1594
Alw21I GWGCWC 1 cut(s) 262
Alw26I GTCTC 4 cut(s) 436, 1016, 1492, 1788
AlwI GGATC 1 cut(s) 1817
Ama87I CYCGRG 2 cut(s) 74, 889
Aor51HI AGCGCT 1 cut(s) 1586
AoxI GGCC 6 cut(s) 329, 393, 695, 1475, 1738, 1764
ApeKI GCWGC 6 cut(s) 478, 1349, 1587, 1754, 1829, 2204
ApoI RAATTY 3 cut(s) 360, 557, 1177
AseI ATTAAT 2 cut(s) 983, 1329
AsiGI ACCGGT 1 cut(s) 781
Asp700I GAANNNNTTC 2 cut(s) 1230, 1443
Asp718I GGTACC 1 cut(s) 1304
AspLEI GCGC 4 cut(s) 298, 722, 1587, 2253
AspS9I GGNCC 3 cut(s) 394, 1765, 1981
AsuC2I CCSGG 2 cut(s) 2100, 2118
AsuHPI GGTGA 7 cut(s) 839, 910, 1276, 1400, 1412, 1442, 2038
AvaI CYCGRG 2 cut(s) 74, 889
AvaII GGWCC 1 cut(s) 1981
AxyI CCTNAGG 1 cut(s) 1479
BaeGI GKGCMC 1 cut(s) 1581
BalI TGGCCA 1 cut(s) 1740
BanI GGYRCC 3 cut(s) 307, 1304, 2077
BbrPI CACGTG 2 cut(s) 992, 1468
BbsI GAAGAC 2 cut(s) 71, 581
Bbv12I GWGCWC 1 cut(s) 262
BbvCI CCTCAGC 1 cut(s) 1560
BbvI GCAGC 6 cut(s) 465, 1336, 1574, 1766, 1816, 2216
BccI CCATC 3 cut(s) 1379, 1429, 1513
BceAI ACGGC 1 cut(s) 278
BciT130I CCWGG 2 cut(s) 377, 1929
BciVI GTATCC 2 cut(s) 373, 2216
BclI TGATCA 1 cut(s) 192
BcnI CCSGG 2 cut(s) 2100, 2118
BcoDI GTCTC 4 cut(s) 436, 1016, 1492, 1788
BcuI ACTAGT 1 cut(s) 1631
BfaI CTAG 4 cut(s) 833, 872, 1091, 1632
BfmI CTRYAG 2 cut(s) 333, 1422
BfoI RGCGCY 1 cut(s) 1588
BfuI GTATCC 2 cut(s) 373, 2216
BglII AGATCT 1 cut(s) 1063
BisI GCNGC 6 cut(s) 479, 1350, 1588, 1755, 1830, 2205
BlsI GCNGC 6 cut(s) 480, 1351, 1589, 1756, 1831, 2206
Bme1390I CCNGG 4 cut(s) 377, 1929, 2100, 2118
Bme18I GGWCC 1 cut(s) 1981
BmeT110I CYCGRG 2 cut(s) 74, 889
BmgT120I GGNCC 3 cut(s) 394, 1765, 1981
BmiI GGNNCC 7 cut(s) 7, 309, 396, 1306, 1864, 1983, 2079
BmrFI CCNGG 4 cut(s) 377, 1929, 2100, 2118
BmsI GCATC 2 cut(s) 1355, 1393
BpiI GAAGAC 2 cut(s) 71, 581
BpmI CTGGAG 1 cut(s) 692
Bpu10I CCTNAGC 1 cut(s) 1560
BpuEI CTTGAG 1 cut(s) 849
BpuMI CCSGG 2 cut(s) 2100, 2118
BsaAI YACGTR 2 cut(s) 992, 1468
BsaJI CCNNGG 4 cut(s) 548, 972, 1741, 1928
BsaWI WCCGGW 1 cut(s) 781
BsaXI ACNNNNNCTCC 2 cut(s) 903, 933
Bsc4I CCNNNNNNNGG 4 cut(s) 488, 1426, 1427, 2014
Bse118I RCCGGY 2 cut(s) 428, 781
Bse1I ACTGG 2 cut(s) 244, 675
Bse21I CCTNAGG 1 cut(s) 1479
BseBI CCWGG 2 cut(s) 377, 1929
BseDI CCNNGG 4 cut(s) 548, 972, 1741, 1928
BseGI GGATG 5 cut(s) 64, 1222, 1370, 1390, 1969
BseLI CCNNNNNNNGG 4 cut(s) 488, 1426, 1427, 2014
BseMII CTCAG 4 cut(s) 497, 581, 1470, 1551
BseNI ACTGG 2 cut(s) 244, 675
BseRI GAGGAG 2 cut(s) 1034, 1496
BseSI GKGCMC 1 cut(s) 1581
BseXI GCAGC 6 cut(s) 465, 1336, 1574, 1766, 1816, 2216
Bsh1236I CGCG 2 cut(s) 298, 720
BshFI GGCC 6 cut(s) 331, 395, 697, 1477, 1740, 1766
BshNI GGYRCC 3 cut(s) 307, 1304, 2077
BshTI ACCGGT 1 cut(s) 781
BsiHKAI GWGCWC 1 cut(s) 262
BsiHKCI CYCGRG 2 cut(s) 74, 889
BsiSI CCGG 4 cut(s) 429, 782, 2099, 2117
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 4 cut(s) 488, 1426, 1427, 2014
BsmAI GTCTC 4 cut(s) 436, 1016, 1492, 1788
BsmBI CGTCTC 1 cut(s) 1788
BsmFI GGGAC 1 cut(s) 57
BsmI GAATGC 2 cut(s) 294, 530
BsnI GGCC 6 cut(s) 331, 395, 697, 1477, 1740, 1766
BsoBI CYCGRG 2 cut(s) 74, 889
Bsp1286I GDGCHC 2 cut(s) 262, 1581
Bsp1407I TGTACA 2 cut(s) 180, 1599
Bsp143I GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
Bsp19I CCATGG 1 cut(s) 548
BspACI CCGC 3 cut(s) 56, 1427, 2036
BspANI GGCC 6 cut(s) 331, 395, 697, 1477, 1740, 1766
BspCNI CTCAG 4 cut(s) 498, 580, 1471, 1552
BspFNI CGCG 2 cut(s) 298, 720
BspHI TCATGA 2 cut(s) 1159, 1620
BspLI GGNNCC 7 cut(s) 7, 309, 396, 1306, 1864, 1983, 2079
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 1 cut(s) 1817
BspT107I GGYRCC 3 cut(s) 307, 1304, 2077
BsrFI RCCGGY 2 cut(s) 428, 781
BsrGI TGTACA 2 cut(s) 180, 1599
BsrI ACTGG 2 cut(s) 244, 675
BssAI RCCGGY 2 cut(s) 428, 781
BssECI CCNNGG 4 cut(s) 548, 972, 1741, 1928
BssMI GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
BssT1I CCWWGG 3 cut(s) 548, 972, 1741
Bst2UI CCWGG 2 cut(s) 377, 1929
Bst4CI ACNGT 4 cut(s) 127, 1309, 1651, 1844
Bst6I CTCTTC 4 cut(s) 620, 765, 812, 1694
BstAPI GCANNNNNTGC 2 cut(s) 271, 478
BstAUI TGTACA 2 cut(s) 180, 1599
BstBAI YACGTR 2 cut(s) 992, 1468
BstC8I GCNNGC 5 cut(s) 294, 329, 333, 430, 1592
BstDEI CTNAG 5 cut(s) 506, 567, 1479, 1560, 2196
BstDSI CCRYGG 1 cut(s) 548
BstF5I GGATG 5 cut(s) 64, 1222, 1370, 1390, 1969
BstFNI CGCG 2 cut(s) 298, 720
BstH2I RGCGCY 1 cut(s) 1588
BstHHI GCGC 4 cut(s) 298, 722, 1587, 2253
BstKTI GATC 8 cut(s) 195, 315, 718, 1066, 1717, 1825, 1853, 1903
BstMAI GTCTC 4 cut(s) 436, 1016, 1492, 1788
BstMBI GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
BstMWI GCNNNNNNNGC 8 cut(s) 271, 478, 520, 949, 1121, 1483, 1591, 1763
BstNI CCWGG 2 cut(s) 377, 1929
BstNSI RCATGY 2 cut(s) 476, 1610
BstSCI CCNGG 4 cut(s) 375, 1927, 2098, 2116
BstSFI CTRYAG 2 cut(s) 333, 1422
BstSLI GKGCMC 1 cut(s) 1581
BstUI CGCG 2 cut(s) 298, 720
BstV1I GCAGC 6 cut(s) 465, 1336, 1574, 1766, 1816, 2216
BstV2I GAAGAC 2 cut(s) 71, 581
BstX2I RGATCY 1 cut(s) 1063
BstXI CCANNNNNNTGG 1 cut(s) 1654
BstYI RGATCY 1 cut(s) 1063
Bsu36I CCTNAGG 1 cut(s) 1479
BsuI GTATCC 2 cut(s) 373, 2216
BsuRI GGCC 6 cut(s) 331, 395, 697, 1477, 1740, 1766
BtgI CCRYGG 1 cut(s) 548
BtgZI GCGATG 1 cut(s) 864
BtsCI GGATG 5 cut(s) 64, 1222, 1370, 1390, 1969
BtsI GCAGTG 2 cut(s) 342, 1278
BtsIMutI CAGTG 5 cut(s) 231, 237, 342, 1278, 1386
Cac8I GCNNGC 5 cut(s) 294, 329, 333, 430, 1592
CciI TCATGA 2 cut(s) 1159, 1620
CfoI GCGC 4 cut(s) 298, 722, 1587, 2253
Cfr10I RCCGGY 2 cut(s) 428, 781
Cfr13I GGNCC 3 cut(s) 394, 1765, 1981
Csp6I GTAC 5 cut(s) 181, 1305, 1600, 1676, 2105
CspAI ACCGGT 1 cut(s) 781
CviAII CATG 9 cut(s) 473, 549, 1160, 1343, 1607, 1621, 1762, 1775, 2013
CviQI GTAC 5 cut(s) 181, 1305, 1600, 1676, 2105
DdeI CTNAG 5 cut(s) 506, 567, 1479, 1560, 2196
DpnI GATC 8 cut(s) 194, 314, 717, 1065, 1716, 1824, 1852, 1902
DpnII GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
DrdI GACNNNNNNGTC 1 cut(s) 1664
DseDI GACNNNNNNGTC 1 cut(s) 1664
EaeI YGGCCR 1 cut(s) 1738
Eam1104I CTCTTC 4 cut(s) 620, 765, 812, 1694
EarI CTCTTC 4 cut(s) 620, 765, 812, 1694
Eco130I CCWWGG 3 cut(s) 548, 972, 1741
Eco32I GATATC 1 cut(s) 1969
Eco47I GGWCC 1 cut(s) 1981
Eco47III AGCGCT 1 cut(s) 1586
Eco57I CTGAAG 1 cut(s) 720
Eco72I CACGTG 2 cut(s) 992, 1468
Eco81I CCTNAGG 1 cut(s) 1479
Eco88I CYCGRG 2 cut(s) 74, 889
EcoRII CCWGG 2 cut(s) 375, 1927
EcoRV GATATC 1 cut(s) 1969
EcoT14I CCWWGG 3 cut(s) 548, 972, 1741
EcoT22I ATGCAT 1 cut(s) 532
ErhI CCWWGG 3 cut(s) 548, 972, 1741
Esp3I CGTCTC 1 cut(s) 1788
FaeI CATG 9 cut(s) 476, 552, 1163, 1346, 1610, 1624, 1765, 1778, 2016
FalI AAGNNNNNCTT 2 cut(s) 443, 475
FaqI GGGAC 1 cut(s) 57
FatI CATG 9 cut(s) 472, 548, 1159, 1342, 1606, 1620, 1761, 1774, 2012
FauI CCCGC 1 cut(s) 1420
FauNDI CATATG 1 cut(s) 1955
FbaI TGATCA 1 cut(s) 192
Fnu4HI GCNGC 6 cut(s) 479, 1350, 1588, 1755, 1830, 2205
FokI GGATG 5 cut(s) 71, 1229, 1377, 1397, 1976
Fsp4HI GCNGC 6 cut(s) 479, 1350, 1588, 1755, 1830, 2205
FspAI RTGCGCAY 1 cut(s) 2252
FspBI CTAG 4 cut(s) 833, 872, 1091, 1632
FspI TGCGCA 1 cut(s) 2252
GlaI GCGC 4 cut(s) 297, 721, 1586, 2252
GluI GCNGC 6 cut(s) 479, 1350, 1588, 1755, 1830, 2205
GsuI CTGGAG 1 cut(s) 692
HaeII RGCGCY 1 cut(s) 1588
HaeIII GGCC 6 cut(s) 331, 395, 697, 1477, 1740, 1766
HapII CCGG 4 cut(s) 429, 782, 2099, 2117
HhaI GCGC 4 cut(s) 298, 722, 1587, 2253
Hin1II CATG 9 cut(s) 476, 552, 1163, 1346, 1610, 1624, 1765, 1778, 2016
Hin6I GCGC 4 cut(s) 296, 720, 1585, 2251
HinP1I GCGC 4 cut(s) 296, 720, 1585, 2251
HincII GTYRAC 2 cut(s) 420, 1567
HindII GTYRAC 2 cut(s) 420, 1567
HindIII AAGCTT 1 cut(s) 941
HinfI GANTC 8 cut(s) 77, 86, 107, 140, 618, 1393, 1876, 2212
HpaII CCGG 4 cut(s) 429, 782, 2099, 2117
HphI GGTGA 7 cut(s) 839, 910, 1276, 1400, 1412, 1442, 2038
Hpy166II GTNNAC 6 cut(s) 340, 420, 746, 930, 1567, 1678
Hpy188I TCNGA 8 cut(s) 82, 570, 965, 1144, 1497, 1855, 2193, 2217
Hpy8I GTNNAC 6 cut(s) 340, 420, 746, 930, 1567, 1678
HpyAV CCTTC 5 cut(s) 10, 547, 695, 1351, 2152
HpyCH4III ACNGT 4 cut(s) 127, 1309, 1651, 1844
HpyCH4IV ACGT 2 cut(s) 991, 1467
HpyF10VI GCNNNNNNNGC 8 cut(s) 271, 478, 520, 949, 1121, 1483, 1591, 1763
HpyF3I CTNAG 5 cut(s) 506, 567, 1479, 1560, 2196
HpySE526I ACGT 2 cut(s) 991, 1467
Hsp92II CATG 9 cut(s) 476, 552, 1163, 1346, 1610, 1624, 1765, 1778, 2016
HspAI GCGC 4 cut(s) 296, 720, 1585, 2251
KpnI GGTACC 1 cut(s) 1308
KroI GCCGGC 1 cut(s) 428
KroNI GCCGGC 1 cut(s) 430
Ksp22I TGATCA 1 cut(s) 192
Kzo9I GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
LmnI GCTCC 3 cut(s) 846, 1346, 1483
Lsp1109I GCAGC 6 cut(s) 465, 1336, 1574, 1766, 1816, 2216
LweI GCATC 2 cut(s) 1355, 1393
MaeI CTAG 4 cut(s) 833, 872, 1091, 1632
MaeII ACGT 2 cut(s) 991, 1467
MaeIII GTNAC 7 cut(s) 127, 223, 540, 562, 775, 1463, 1783
MalI GATC 8 cut(s) 194, 314, 717, 1065, 1716, 1824, 1852, 1902
MboI GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
MflI RGATCY 1 cut(s) 1063
MhlI GDGCHC 2 cut(s) 262, 1581
MlsI TGGCCA 1 cut(s) 1740
MluNI TGGCCA 1 cut(s) 1740
MlyI GAGTC 4 cut(s) 86, 95, 627, 2206
MmeI TCCRAC 4 cut(s) 789, 889, 996, 2216
Mox20I TGGCCA 1 cut(s) 1740
Mph1103I ATGCAT 1 cut(s) 532
MroNI GCCGGC 1 cut(s) 428
MroXI GAANNNNTTC 2 cut(s) 1230, 1443
MscI TGGCCA 1 cut(s) 1740
MseI TTAA 7 cut(s) 461, 597, 939, 983, 1070, 1329, 2040
MslI CAYNNNNRTG 4 cut(s) 287, 1632, 1773, 2258
Msp20I TGGCCA 1 cut(s) 1740
MspI CCGG 4 cut(s) 429, 782, 2099, 2117
MspR9I CCNGG 4 cut(s) 377, 1929, 2100, 2118
Mva1269I GAATGC 2 cut(s) 294, 530
MvaI CCWGG 2 cut(s) 377, 1929
MvnI CGCG 2 cut(s) 298, 720
MwoI GCNNNNNNNGC 8 cut(s) 271, 478, 520, 949, 1121, 1483, 1591, 1763
NaeI GCCGGC 1 cut(s) 430
NciI CCSGG 2 cut(s) 2100, 2118
NcoI CCATGG 1 cut(s) 548
NdeI CATATG 1 cut(s) 1955
NdeII GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
NgoMIV GCCGGC 1 cut(s) 428
NlaIII CATG 9 cut(s) 476, 552, 1163, 1346, 1610, 1624, 1765, 1778, 2016
NlaIV GGNNCC 7 cut(s) 7, 309, 396, 1306, 1864, 1983, 2079
NmuCI GTSAC 4 cut(s) 127, 223, 775, 1463
NsbI TGCGCA 1 cut(s) 2252
NsiI ATGCAT 1 cut(s) 532
NspI RCATGY 2 cut(s) 476, 1610
PagI TCATGA 2 cut(s) 1159, 1620
PctI GAATGC 2 cut(s) 294, 530
PdiI GCCGGC 1 cut(s) 430
PdmI GAANNNNTTC 2 cut(s) 1230, 1443
PfeI GAWTC 4 cut(s) 107, 140, 1393, 1876
PflFI GACNNNGTC 1 cut(s) 125
PfoI TCCNGGA 1 cut(s) 375
PinAI ACCGGT 1 cut(s) 781
PkrI GCNGC 6 cut(s) 480, 1351, 1589, 1756, 1831, 2206
PleI GAGTC 4 cut(s) 85, 94, 626, 2206
PmaCI CACGTG 2 cut(s) 992, 1468
PmlI CACGTG 2 cut(s) 992, 1468
PpsI GAGTC 4 cut(s) 85, 94, 626, 2206
Ppu21I YACGTR 2 cut(s) 992, 1468
PshBI ATTAAT 2 cut(s) 983, 1329
PsiI TTATAA 1 cut(s) 2168
Psp6I CCWGG 2 cut(s) 375, 1927
PspCI CACGTG 2 cut(s) 992, 1468
PspGI CCWGG 2 cut(s) 375, 1927
PspN4I GGNNCC 7 cut(s) 7, 309, 396, 1306, 1864, 1983, 2079
PspPI GGNCC 3 cut(s) 394, 1765, 1981
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 1063
PsyI GACNNNGTC 1 cut(s) 125
RsaI GTAC 5 cut(s) 182, 1306, 1601, 1677, 2106
RsaNI GTAC 5 cut(s) 181, 1305, 1600, 1676, 2105
RseI CAYNNNNRTG 4 cut(s) 287, 1632, 1773, 2258
SaqAI TTAA 7 cut(s) 461, 597, 939, 983, 1070, 1329, 2040
SatI GCNGC 6 cut(s) 479, 1350, 1588, 1755, 1830, 2205
Sau3AI GATC 8 cut(s) 192, 312, 715, 1063, 1714, 1822, 1850, 1900
Sau96I GGNCC 3 cut(s) 394, 1765, 1981
SchI GAGTC 4 cut(s) 86, 95, 627, 2206
ScrFI CCNGG 4 cut(s) 377, 1929, 2100, 2118
SduI GDGCHC 2 cut(s) 262, 1581
SfaNI GCATC 2 cut(s) 1355, 1393
SfcI CTRYAG 2 cut(s) 333, 1422
SinI GGWCC 1 cut(s) 1981
SmiMI CAYNNNNRTG 4 cut(s) 287, 1632, 1773, 2258
SmlI CTYRAG 1 cut(s) 864
SmoI CTYRAG 1 cut(s) 864
SpeI ACTAGT 1 cut(s) 1631
SsiI CCGC 3 cut(s) 56, 1427, 2036
SspMI CTAG 4 cut(s) 833, 872, 1091, 1632
StyD4I CCNGG 4 cut(s) 375, 1927, 2098, 2116
StyI CCWWGG 3 cut(s) 548, 972, 1741
TaaI ACNGT 4 cut(s) 127, 1309, 1651, 1844
TaiI ACGT 2 cut(s) 994, 1470
TaqI TCGA 2 cut(s) 1314, 1447
TatI WGTACW 2 cut(s) 180, 1599
TfiI GAWTC 4 cut(s) 107, 140, 1393, 1876
Tru1I TTAA 7 cut(s) 461, 597, 939, 983, 1070, 1329, 2040
Tru9I TTAA 7 cut(s) 461, 597, 939, 983, 1070, 1329, 2040
TscAI CASTG 5 cut(s) 238, 244, 342, 1278, 1386
TseFI GTSAC 4 cut(s) 127, 223, 775, 1463
TseI GCWGC 6 cut(s) 478, 1349, 1587, 1754, 1829, 2204
Tsp45I GTSAC 4 cut(s) 127, 223, 775, 1463
TspDTI ATGAA 5 cut(s) 816, 1148, 1681, 1791, 2171
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 5 cut(s) 238, 244, 342, 1278, 1386
Tth111I GACNNNGTC 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 1981
VspI ATTAAT 2 cut(s) 983, 1329
XapI RAATTY 3 cut(s) 360, 557, 1177
XceI RCATGY 2 cut(s) 476, 1610
XcmI CCANNNNNNNNNTGG 1 cut(s) 1935
XmnI GAANNNNTTC 2 cut(s) 1230, 1443
XspI CTAG 4 cut(s) 833, 872, 1091, 1632
Zsp2I ATGCAT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.