FvH4_6g04261

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000126
Physical Location & Seq
Forward (+)
2318553 .. 2325602
7050 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04261.t2

Sequence Viewer

Length: 3150 bp
ATGGAGAAGATAGCATCCGATTTGAAGGCATCGGAGACGAAGCAAAGGAGTCTAGGCAAGACGTACGAGTCATTGCATTCCCAAGCGTCTTCCATGCTCGTTCTCTCCATTCAATGGAAGGATCTCCAGGAACACTTTGATTCGACCCGGAATGCGATCCGGACCTGTTTCGAAGAGCTTCGGGAGCGAGAGAAGATGTTGGAAGCCAAGGAGTTAAAATTCAAGTCGGAGATGGAGTCCAAAACGGACGAGTTGTGTGGAGTTCAGAAGTTGGTTGATGAGAAAGTGCAAGAGGTTATAGAATTAAAGAGATCGATTCAGAAGCATAGCCAGGAGATTGAACGTGAAGAGAAGAGAGTGATGGATGTACAGAAATTGGTTGTAGACAAGGAGAGGGAGTGTGGTGTGGTGGAAAGAAGGATTCAAGAGCGTACCAAGAAACTGAATTGGGTTGAGAGAAGGATTGAAGAGAAATTGAATGAGGTTGAGTCTAAGGAGAAGGAACTGAACAAGTACCGTGAGGATATTAAGGTGAAAATGGAGCAGTTAGGTGTGATTGATGAGACCATTGTGGAGGGTAAGGAAAAGATCGAGTCAAAAGAAGAGGAAATAAAAGCGGCTCAGATGCTGTTAGAAGAATGTGAGAGAAGAATAAAATCGAAAGAGGAGAAATTGAGTTGGATAGAGAAGAGTATAGAAGACACTTCCAAGTTGGCCAACCTTAAGGAAGAGGAAGTGAGAGTGATACAAGGATCGTTGAATGTATATCGCGAGAGTATTAAGTCGAAAGAGGGTGAATTAGATGCGATTCTTGAGTCAATTGAAGGGAAGAAGAGAGAATTTGATGTGAAAGAAGAGCAAATCAAAACGTTGCAGAGATTGATTGAAGGATGTGAGAAAGAGCTCAAATCGAAAGAGGAGAAATTGAAAGAGGAGACGCTCAAAGTGAAAGAGTATAGCTTGCGAAATCGACCAATGGAGGAGTGGTCCTGCAAGCTTGAACTTAAAGAGAGGGAAGTTGAATTGAAAGAGAAACAAGTAGAGTCAAAAATGGAGGAATTTAAAAAATGGGTTGAAGAACGCGGAAAGGAATTGAATTCACTTTCCTCGGAACTTAAAGTTAAAGAGGGGCAACTTCAACAGGAGGCCAAAGATCTTGAGTTGGCAAAGAAACAATGTGGTGTTGTTGCTCAAGTGAACAATGCCATTGTTCCTTCATCAGCAAGTGATCAATCAAACATCAATTTTACTGATGGAAGAAATTTGCAGCAGTTCATGTATGAGCATATGAAGAGAAATGATTCAATGAGCAACAAAATCTCTTCTATTCTTCAACAAGAGTCATCAGAACCAGGAAAACTGGTTTTGGATGCAATGCAAGGGTTTTACCCTTCAAACACGACCGTCGAGAACAACGAGTTTGATTTGGTGCTTATTAGAAGGACATGCATTCTTTTGTTAGAAGAATTAAAGAGAGCCTCGCCCCAAATTAATCCGGAGTTGATAGAAGAAGCAATGAAGTTGGCCAGTGATTGGAAGGCTAAGATGAAAGTGGGGAGTGAAAATTGGTTGGAGATTTTGGGTTTTTTGCGTCTTGTTACTACCTATGAATTGACCTCTGCTTATGATAGGAAGGAGCTCCAAAGTCTTTTTCACGTAGTTGCTCAGCATGAACAAGCAATGGAATTATTCCGGGCCCTTGGTACCACATATAAGGCACCTGATTATGGCAAGATTTGTTCCTCTGTAAAAACTGAAAAACCAGAATCTCAAGTAGCCAAAAATGAAGCAACTTCTTCAAATCTTCAGGTCACTGCCACCACTACTGATGCAACGCATTTGCAGGGTCTTCAAAATGAGCAGTTTCGTGGTATTATTTATGATCTTATAAAAAAGAAGCGACTGATGGACGCTGCTGGATTGATTTACACCTTGAAGTTATTTGACGAGTTTCCCCCAGTACAAGTCTTGAAAGAATATGTTGATAAGGGTAATAAGTGCTGCAGGAATAGAACCAAGAGAAAGAAATTGCTGGATGAAAAGGATAAGATTACAGACCAGCATATAGCAGATTTACGAGTTGTTATTCAATGTATCAAAGCTTACAACCTCGAGTCTGAATACCCACCAGAGGAAATTGAATCACAAATAGCTCTGCTTGAAAGGTCTAAGAAGCAGAGACGCGTAGCGTCATCATCTCTTGTTTTAGAGGTTGAACAACAACAGCAAACGTCGCTGAAAATTGTTGAATTTCAAGAACAACAAGAACTGCAGAAAACCTCCAATGCTAAACTACAACAAAAGGCAGATCAGAAAGCCTCCAAGGTTGAACAACAAGAACAGCAACAAGAGCAGAAAACATCCAAGGGTAAACTGCAAGAAAAAGTGGAGCAGAAAGCCTCCGATAAGGTTGAAAGCCAACAACAGGTACAGGAGAAGATAGCCTCCAAGATCGAACAATTGCTACGAGAGCAGAAAACCCTCAAGGCTAGACTACAAGAGAAAGTAGAGCAGAAAGCCTGCAATAAGGTTGAAAAACGAAAACAGCTACAAGAGCAGAAAGCCTCCGAGTATGAACAACAAGAACAGCAGAAAACCTCCAAGGCTAAACTGGAAGAAAAAGTAGAGCAGAAAGCCTCTGATAAGGTTGAAGAACAACAACAAAAGCAAGAGCAGAACACCTCCAAGGCTACACTACAAGAAAAAGTAGAGCAGAAAGCCTCCAACCAGGTTGAAGAACAACAACAGCAACAAGAGCATCAGAACGCCTCCAAGGTTAAACCGCAAGAACAACCAAAGCAGAAAAAGGGCAATAAACGTTCGCACATCACATTTGCTACCAGGTTTGACCACCAACAGCAGCTTCAAAATAAATCTCAAAGAACATCTGTAGTAGCTAATAGACCGATCAGATTGCACCAAGACCATCCTGACTATCCAAATTCAAGTTCATCAACTTGGCCACATGAAAGATATGGATACCATGGACAGTTTGGTACTCCTTTCAACAACTATCATGTTCATGCTAACTACGGACATCCTGTGCAGTTTGGAATTCCTATCAGTCGTAGAGCTAATTATGGATATGGTGGACATAATCCCGGATTAAATCAGTCGTATCCCTACCTTCCTCGACATCCTCCTCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

1050

Amino Acids

122.28

Weight (kDa)

7.75

Isoelectric Point (pI)

59.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 418 - 575 4e-32 Frigida-like protein
Frigida PF07899 615 - 728 5.3e-21 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000126)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04990
fragaria_vesca FvH4_5g20491 FvH4_5g20491 FvH4_5g20491 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04261 FvH4_6g04310 FvH4_6g04310 FvH4_6g04320 FvH4_6g04320 FvH4_6g04380 FvH4_6g04380 FvH4_6g04410 FvH4_6g04410 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04420 FvH4_6g04421 FvH4_6g04421 FvH4_6g04421 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04431 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04550 FvH4_6g04561 FvH4_6g04562 FvH4_7g18700
malus_domestica MD04G1214600.v1.1 MD04G1214700.v1.1 MD12G1013700.v1.1 MD12G1229200.v1.1 MD12G1229900.v1.1 MD12G1230000.v1.1 MD14G1011100.v1.1
prunus_persica Prupe.1G243600_v2.0.a1 Prupe.6G334600_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.6G334700_v2.0.a1 Prupe.7G018200_v2.0.a1
pyrus_communis pycom03g04860 pycom04g18970 pycom04g18980 pycom11g05330 pycom11g05340 pycom11g05350 pycom12g01100 pycom12g01110 pycom12g21380 pycom14g00990
rosa_chinensis RchiOBHm_Chr2g0124181 RchiOBHm_Chr3g0452971 RchiOBHm_Chr3g0452981 RchiOBHm_Chr3g0452991 RchiOBHm_Chr3g0453041 RchiOBHm_Chr3g0453051 RchiOBHm_Chr3g0453061 RchiOBHm_Chr3g0453081 RchiOBHm_Chr3g0453091 RchiOBHm_Chr4g0411451 RchiOBHm_Chr7g0205851 RchiOBHm_Chr7g0237441
rosa_laevigata RLG00000001002 RLG00000003350 RLG00000008356 RLG00000018794 RLG00000025538 RLG00000025541 RLG00000025542 RLG00000025544 RLG00000025545 RLG00000025552 RLG00000025553 RLG00000025555 RLG00000025557
rosa_multiflora Rmu_co8055994.1_g000001 Rmu_co8332235.1_g000001 Rmu_co8359511.1_g000001 Rmu_sc0000076.1_g000037 Rmu_sc0000832.1_g000001 Rmu_sc0001982.1_g000017 Rmu_sc0002843.1_g000008 Rmu_sc0002923.1_g000015 Rmu_sc0002923.1_g000020 Rmu_sc0005888.1_g000004 Rmu_sc0005888.1_g000007 Rmu_sc0005888.1_g000012 Rmu_sc0005888.1_g000013 Rmu_sc0005888.1_g000014 Rmu_sc0005888.1_g000015 Rmu_sc0007863.1_g000006 Rmu_sc0008518.1_g000002 Rmu_sc0008797.1_g000002 Rmu_sc0008797.1_g000003 Rmu_sc0008797.1_g000007 Rmu_sc0009015.1_g000007 Rmu_sc0009015.1_g000008 Rmu_sc0012369.1_g000002 Rmu_sc0013935.1_g000001 Rmu_sc0019895.1_g000001 Rmu_sc0023319.1_g000001 Rmu_sc0030229.1_g000001 Rmu_sc0033390.1_g000001 Rmu_sc0042937.1_g000001 Rmu_ssc0000052.1_g000010
rosa_roxburghii Rroxscaffold_3G00224200 Rroxscaffold_3G00251930 Rroxscaffold_5G00356050 Rroxscaffold_6G00429980 Rroxscaffold_6G00430030 Rroxscaffold_6G00430040 Rroxscaffold_6G00430070 Rroxscaffold_6G00430080 Rroxscaffold_6G00430090 Rroxscaffold_6G00430130 Rroxscaffold_6G00430140 Rroxscaffold_6G00430160
rosa_rugosa Rorug02G0250200 Rorug02G0643800 Rorug02G0643900 Rorug02G0644000 Rorug02G0644100 Rorug02G0644200 Rorug02G0644500 Rorug02G0644600 Rorug02G0644800 Rorug02G0644900 Rorug02G0645000 Rorug02G0645800 Rorug02G0645900 Rorug02G0646200 Rorug02G0646400.1 Rorug04G0108800 Rorug07G0093400 Rorug07G0303400 Rorug07G0303500 Rorug07G0303600 Rorug07G0303600 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700 Rorug07G0303700
rosa_samantha Rh2AG308300 Rh2BG317200 Rh2DG332300 Rh3AG047000 Rh3AG047100 Rh3AG047200 Rh3AG047300 Rh3AG047400 Rh3AG048600 Rh3AG048700 Rh3AG048900 Rh3AG049000 Rh3BG049400 Rh3BG049500 Rh3BG049600 Rh3BG049700 Rh3BG049900 Rh3BG050200 Rh3CG048100 Rh3CG048200 Rh3CG048400 Rh3CG048500 Rh3CG048700 Rh3CG049800 Rh3CG049900 Rh3CG050200 Rh3CG050300 Rh3DG049000 Rh3DG049100 Rh3DG049300 Rh3DG049400 Rh3DG049500 Rh3DG050600 Rh3DG050700 Rh3DG050900 Rh3DG051000 Rh4AG168200 Rh4BG165100 Rh4CG179700 Rh4DG162700 Rh5BG257600 Rh7AG225000 Rh7AG460700 Rh7BG220200 Rh7BG430500 Rh7CG238600 Rh7CG477900 Rh7DG047500 Rh7DG231700 Rh7DG447300
rosa_wichuraiana Rw2G005470 Rw2G024840 Rw3G003690 Rw3G003700 Rw3G003710 Rw3G003740 Rw3G003750 Rw4G013890 Rw4G013980 Rw7G019410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1889
AasI GACNNNNNNGTC 1 cut(s) 67
Acc65I GGTACC 1 cut(s) 1703
AccB1I GGYRCC 2 cut(s) 1703, 1717
AccB7I CCANNNNNTGG 2 cut(s) 114, 1533
AccI GTMKAC 1 cut(s) 384
AccII CGCG 3 cut(s) 771, 1083, 2184
AccIII TCCGGA 2 cut(s) 159, 1495
AciI CCGC 3 cut(s) 617, 1083, 2783
AclI AACGTT 2 cut(s) 869, 2818
AclWI GGATC 3 cut(s) 129, 151, 760
AcoI YGGCCR 3 cut(s) 714, 1524, 2960
AcsI RAATTY 8 cut(s) 218, 839, 1058, 1096, 1261, 2249, 2941, 3054
AcuI CTGAAG 1 cut(s) 1790
AfaI GTAC 8 cut(s) 65, 369, 433, 515, 1705, 1962, 2430, 2998
AfiI CCNNNNNNNGG 5 cut(s) 114, 1533, 1727, 2131, 2425
AflII CTTAAG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 2182
AjnI CCWGG 5 cut(s) 126, 330, 1351, 2727, 2840
AjuI GAANNNNNNNTTGG 6 cut(s) 1635, 1667, 2847, 2879, 2932, 2964
Alw21I GWGCWC 2 cut(s) 906, 1641
Alw26I GTCTC 4 cut(s) 29, 557, 929, 2173
AlwI GGATC 3 cut(s) 129, 151, 760
AlwNI CAGNNNCTG 1 cut(s) 628
Ama87I CYCGRG 1 cut(s) 2111
Aor13HI TCCGGA 2 cut(s) 159, 1495
AoxI GGCC 5 cut(s) 714, 1146, 1524, 1695, 2960
ApaI GGGCCC 1 cut(s) 1699
ApeKI GCWGC 4 cut(s) 1267, 1913, 2001, 2860
ApoI RAATTY 8 cut(s) 218, 839, 1058, 1096, 1261, 2249, 2941, 3054
AseI ATTAAT 1 cut(s) 1491
Asp700I GAANNNNTTC 2 cut(s) 177, 1300
Asp718I GGTACC 1 cut(s) 1703
AspS9I GGNCC 4 cut(s) 162, 987, 1695, 1696
AsuC2I CCSGG 3 cut(s) 148, 1694, 3102
AsuHPI GGTGA 2 cut(s) 544, 806
AsuII TTCGAA 1 cut(s) 171
AvaI CYCGRG 1 cut(s) 2111
AvaII GGWCC 2 cut(s) 162, 987
BaeGI GKGCMC 1 cut(s) 1699
BalI TGGCCA 3 cut(s) 716, 1526, 2962
BanI GGYRCC 2 cut(s) 1703, 1717
BanII GRGCYC 3 cut(s) 906, 1641, 1699
BbsI GAAGAC 3 cut(s) 81, 705, 1841
Bbv12I GWGCWC 2 cut(s) 906, 1641
BbvI GCAGC 4 cut(s) 1279, 1900, 1988, 2872
BccI CCATC 5 cut(s) 226, 355, 1247, 1900, 2934
BcgI CGANNNNNNTGC 2 cut(s) 2896, 2930
BciT130I CCWGG 5 cut(s) 128, 332, 1353, 2729, 2842
BciVI GTATCC 2 cut(s) 2972, 3129
BclI TGATCA 1 cut(s) 1228
BcnI CCSGG 3 cut(s) 148, 1694, 3102
BcoDI GTCTC 4 cut(s) 29, 557, 929, 2173
BfaI CTAG 2 cut(s) 53, 2490
BfmI CTRYAG 3 cut(s) 2002, 2270, 2889
BfrI CTTAAG 1 cut(s) 722
BfuI GTATCC 2 cut(s) 2972, 3129
BglII AGATCT 1 cut(s) 1153
BisI GCNGC 5 cut(s) 618, 1268, 1914, 2002, 2861
BlpI GCTNAGC 1 cut(s) 1665
BlsI GCNGC 5 cut(s) 619, 1269, 1915, 2003, 2862
Bme1390I CCNGG 8 cut(s) 128, 148, 332, 1353, 1694, 2729, 2842, 3102
Bme18I GGWCC 2 cut(s) 162, 987
BmeT110I CYCGRG 1 cut(s) 2111
BmgT120I GGNCC 4 cut(s) 162, 987, 1695, 1696
BmiI GGNNCC 3 cut(s) 1697, 1705, 1719
BmrFI CCNGG 8 cut(s) 128, 148, 332, 1353, 1694, 2729, 2842, 3102
BmrI ACTGGG 1 cut(s) 1952
BmsI GCATC 7 cut(s) 23, 38, 615, 793, 1360, 1819, 2767
BmuI ACTGGG 1 cut(s) 1952
BpiI GAAGAC 3 cut(s) 81, 705, 1841
BpmI CTGGAG 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 1665
Bpu14I TTCGAA 1 cut(s) 171
BpuEI CTTGAG 5 cut(s) 833, 1176, 1178, 1755, 2468
BpuMI CCSGG 3 cut(s) 148, 1694, 3102
Bsa29I ATCGAT 1 cut(s) 314
BsaAI YACGTR 1 cut(s) 1657
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 9 cut(s) 207, 1107, 1699, 2322, 2364, 2601, 2685, 2772, 2983
BsaWI WCCGGW 2 cut(s) 159, 1495
BsaXI ACNNNNNCTCC 6 cut(s) 221, 251, 383, 413, 971, 1001
Bsc4I CCNNNNNNNGG 5 cut(s) 114, 1533, 1727, 2131, 2425
Bse1I ACTGG 4 cut(s) 1365, 1527, 1958, 2616
Bse3DI GCAATG 4 cut(s) 71, 1380, 1521, 1686
BseAI TCCGGA 2 cut(s) 159, 1495
BseBI CCWGG 5 cut(s) 128, 332, 1353, 2729, 2842
BseCI ATCGAT 1 cut(s) 314
BseDI CCNNGG 9 cut(s) 207, 1107, 1699, 2322, 2364, 2601, 2685, 2772, 2983
BseGI GGATG 9 cut(s) 14, 370, 896, 1375, 2041, 2360, 2926, 3037, 3136
BseLI CCNNNNNNNGG 5 cut(s) 114, 1533, 1727, 2131, 2425
BseMI GCAATG 4 cut(s) 71, 1380, 1521, 1686
BseMII CTCAG 2 cut(s) 635, 1679
BseNI ACTGG 4 cut(s) 1365, 1527, 1958, 2616
BseRI GAGGAG 5 cut(s) 680, 932, 947, 995, 3132
BseSI GKGCMC 1 cut(s) 1699
BseXI GCAGC 4 cut(s) 1279, 1900, 1988, 2872
BsgI GTGCAG 1 cut(s) 3065
Bsh1236I CGCG 3 cut(s) 771, 1083, 2184
Bsh1285I CGRYCG 1 cut(s) 1404
BshFI GGCC 5 cut(s) 716, 1148, 1526, 1697, 2962
BshNI GGYRCC 2 cut(s) 1703, 1717
BshVI ATCGAT 1 cut(s) 314
BsiEI CGRYCG 1 cut(s) 1404
BsiHKAI GWGCWC 2 cut(s) 906, 1641
BsiHKCI CYCGRG 1 cut(s) 2111
BsiSI CCGG 5 cut(s) 148, 160, 1496, 1693, 3102
BsiWI CGTACG 1 cut(s) 63
BslI CCNNNNNNNGG 5 cut(s) 114, 1533, 1727, 2131, 2425
BsmAI GTCTC 4 cut(s) 29, 557, 929, 2173
BsmBI CGTCTC 3 cut(s) 29, 929, 2173
BsmI GAATGC 3 cut(s) 76, 157, 1449
BsnI GGCC 5 cut(s) 716, 1148, 1526, 1697, 2962
Bso31I GGTCTC 1 cut(s) 557
BsoBI CYCGRG 1 cut(s) 2111
Bsp119I TTCGAA 1 cut(s) 171
Bsp120I GGGCCC 1 cut(s) 1695
Bsp1286I GDGCHC 3 cut(s) 906, 1641, 1699
Bsp13I TCCGGA 2 cut(s) 159, 1495
Bsp1407I TGTACA 1 cut(s) 367
Bsp1720I GCTNAGC 1 cut(s) 1665
Bsp19I CCATGG 1 cut(s) 2983
Bsp68I TCGCGA 1 cut(s) 771
BspACI CCGC 3 cut(s) 617, 1083, 2783
BspANI GGCC 5 cut(s) 716, 1148, 1526, 1697, 2962
BspCNI CTCAG 2 cut(s) 634, 1678
BspDI ATCGAT 1 cut(s) 314
BspEI TCCGGA 2 cut(s) 159, 1495
BspFNI CGCG 3 cut(s) 771, 1083, 2184
BspLI GGNNCC 3 cut(s) 1697, 1705, 1719
BspMAI CTGCAG 2 cut(s) 2006, 2274
BspPI GGATC 3 cut(s) 129, 151, 760
BspQI GCTCTTC 2 cut(s) 168, 849
BspT104I TTCGAA 1 cut(s) 171
BspT107I GGYRCC 2 cut(s) 1703, 1717
BspTI CTTAAG 1 cut(s) 722
BspTNI GGTCTC 1 cut(s) 557
BsrDI GCAATG 4 cut(s) 71, 1380, 1521, 1686
BsrGI TGTACA 1 cut(s) 367
BsrI ACTGG 4 cut(s) 1365, 1527, 1958, 2616
BssECI CCNNGG 9 cut(s) 207, 1107, 1699, 2322, 2364, 2601, 2685, 2772, 2983
BssT1I CCWWGG 8 cut(s) 207, 1699, 2322, 2364, 2601, 2685, 2772, 2983
Bst2UI CCWGG 5 cut(s) 128, 332, 1353, 2729, 2842
Bst4CI ACNGT 3 cut(s) 518, 1405, 2991
BstAFI CTTAAG 1 cut(s) 722
BstAUI TGTACA 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 1657
BstBI TTCGAA 1 cut(s) 171
BstC8I GCNNGC 3 cut(s) 962, 995, 2521
BstDEI CTNAG 5 cut(s) 492, 621, 1542, 1665, 2169
BstDSI CCRYGG 1 cut(s) 2983
BstF5I GGATG 9 cut(s) 14, 370, 896, 1375, 2041, 2360, 2926, 3037, 3136
BstFNI CGCG 3 cut(s) 771, 1083, 2184
BstMAI GTCTC 4 cut(s) 29, 557, 929, 2173
BstMCI CGRYCG 1 cut(s) 1404
BstMWI GCNNNNNNNGC 6 cut(s) 184, 2233, 2350, 2470, 2554, 2755
BstNI CCWGG 5 cut(s) 128, 332, 1353, 2729, 2842
BstNSI RCATGY 1 cut(s) 1449
BstSCI CCNGG 8 cut(s) 126, 146, 330, 1351, 1692, 2727, 2840, 3100
BstSFI CTRYAG 3 cut(s) 2002, 2270, 2889
BstSLI GKGCMC 1 cut(s) 1699
BstUI CGCG 3 cut(s) 771, 1083, 2184
BstV1I GCAGC 4 cut(s) 1279, 1900, 1988, 2872
BstV2I GAAGAC 3 cut(s) 81, 705, 1841
BstX2I RGATCY 2 cut(s) 121, 1153
BstYI RGATCY 2 cut(s) 121, 1153
Bsu15I ATCGAT 1 cut(s) 314
BsuI GTATCC 2 cut(s) 2972, 3129
BsuRI GGCC 5 cut(s) 716, 1148, 1526, 1697, 2962
BsuTUI ATCGAT 1 cut(s) 314
BtgI CCRYGG 1 cut(s) 2983
BtsCI GGATG 9 cut(s) 14, 370, 896, 1375, 2041, 2360, 2926, 3037, 3136
BtsI GCAGTG 1 cut(s) 1812
BtsIMutI CAGTG 2 cut(s) 1534, 1812
BtuMI TCGCGA 1 cut(s) 771
Cac8I GCNNGC 3 cut(s) 962, 995, 2521
CaiI CAGNNNCTG 1 cut(s) 628
Cfr13I GGNCC 4 cut(s) 162, 987, 1695, 1696
ClaI ATCGAT 1 cut(s) 314
CseI GACGC 6 cut(s) 75, 946, 1580, 1919, 2178, 2190
CsiI ACCWGGT 2 cut(s) 2727, 2840
Csp6I GTAC 8 cut(s) 64, 368, 432, 514, 1704, 1961, 2429, 2997
CviAII CATG 8 cut(s) 94, 1276, 1446, 1670, 2966, 2984, 3017, 3023
CviQI GTAC 8 cut(s) 64, 368, 432, 514, 1704, 1961, 2429, 2997
DdeI CTNAG 5 cut(s) 492, 621, 1542, 1665, 2169
DraI TTTAAA 1 cut(s) 1063
DrdI GACNNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 1 cut(s) 67
EaeI YGGCCR 3 cut(s) 714, 1524, 2960
Ecl136II GAGCTC 2 cut(s) 904, 1639
Eco130I CCWWGG 8 cut(s) 207, 1699, 2322, 2364, 2601, 2685, 2772, 2983
Eco24I GRGCYC 3 cut(s) 906, 1641, 1699
Eco31I GGTCTC 1 cut(s) 557
Eco47I GGWCC 2 cut(s) 162, 987
Eco53kI GAGCTC 2 cut(s) 904, 1639
Eco57I CTGAAG 1 cut(s) 1790
Eco88I CYCGRG 1 cut(s) 2111
EcoICRI GAGCTC 2 cut(s) 904, 1639
EcoO109I RGGNCCY 1 cut(s) 1696
EcoRI GAATTC 2 cut(s) 1096, 3054
EcoRII CCWGG 5 cut(s) 126, 330, 1351, 2727, 2840
EcoT14I CCWWGG 8 cut(s) 207, 1699, 2322, 2364, 2601, 2685, 2772, 2983
EcoT22I ATGCAT 1 cut(s) 1451
EcoT38I GRGCYC 3 cut(s) 906, 1641, 1699
ErhI CCWWGG 8 cut(s) 207, 1699, 2322, 2364, 2601, 2685, 2772, 2983
Esp3I CGTCTC 3 cut(s) 29, 929, 2173
FaeI CATG 8 cut(s) 97, 1279, 1449, 1673, 2969, 2987, 3020, 3026
FatI CATG 8 cut(s) 93, 1275, 1445, 1669, 2965, 2983, 3016, 3022
FauNDI CATATG 1 cut(s) 1287
FbaI TGATCA 1 cut(s) 1228
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 618, 1268, 1914, 2002, 2861
FokI GGATG 8 cut(s) 377, 903, 1382, 2048, 2347, 2913, 3024, 3123
FriOI GRGCYC 3 cut(s) 906, 1641, 1699
Fsp4HI GCNGC 5 cut(s) 618, 1268, 1914, 2002, 2861
FspBI CTAG 2 cut(s) 53, 2490
GluI GCNGC 5 cut(s) 618, 1268, 1914, 2002, 2861
GsuI CTGGAG 1 cut(s) 110
HaeIII GGCC 5 cut(s) 716, 1148, 1526, 1697, 2962
HapII CCGG 5 cut(s) 148, 160, 1496, 1693, 3102
HgaI GACGC 6 cut(s) 75, 946, 1580, 1919, 2178, 2190
Hin1II CATG 8 cut(s) 97, 1279, 1449, 1673, 2969, 2987, 3020, 3026
HindIII AAGCTT 2 cut(s) 995, 2100
HpaII CCGG 5 cut(s) 148, 160, 1496, 1693, 3102
HphI GGTGA 2 cut(s) 544, 806
Hpy166II GTNNAC 4 cut(s) 385, 1198, 2372, 3092
Hpy8I GTNNAC 4 cut(s) 385, 1198, 2372, 3092
Hpy99I CGWCG 2 cut(s) 1409, 2236
HpyCH4III ACNGT 3 cut(s) 518, 1405, 2991
HpyCH4IV ACGT 6 cut(s) 62, 343, 869, 1656, 2231, 2818
HpyF10VI GCNNNNNNNGC 6 cut(s) 184, 2233, 2350, 2470, 2554, 2755
HpyF3I CTNAG 5 cut(s) 492, 621, 1542, 1665, 2169
HpySE526I ACGT 6 cut(s) 62, 343, 869, 1656, 2231, 2818
Hsp92II CATG 8 cut(s) 97, 1279, 1449, 1673, 2969, 2987, 3020, 3026
Kpn2I TCCGGA 2 cut(s) 159, 1495
KpnI GGTACC 1 cut(s) 1707
Ksp22I TGATCA 1 cut(s) 1228
LguI GCTCTTC 2 cut(s) 168, 849
LmnI GCTCC 5 cut(s) 184, 541, 1636, 1644, 2389
Lsp1109I GCAGC 4 cut(s) 1279, 1900, 1988, 2872
LweI GCATC 7 cut(s) 23, 38, 615, 793, 1360, 1819, 2767
MabI ACCWGGT 2 cut(s) 2727, 2840
MaeI CTAG 2 cut(s) 53, 2490
MaeII ACGT 6 cut(s) 62, 343, 869, 1656, 2231, 2818
MaeIII GTNAC 2 cut(s) 1597, 1810
MfeI CAATTG 2 cut(s) 819, 2459
MflI RGATCY 2 cut(s) 121, 1153
MhlI GDGCHC 3 cut(s) 906, 1641, 1699
MlsI TGGCCA 3 cut(s) 716, 1526, 2962
MluI ACGCGT 1 cut(s) 2182
MluNI TGGCCA 3 cut(s) 716, 1526, 2962
MlyI GAGTC 9 cut(s) 58, 77, 245, 497, 602, 824, 1052, 1349, 2123
MmeI TCCRAC 5 cut(s) 180, 207, 659, 1551, 2748
Mox20I TGGCCA 3 cut(s) 716, 1526, 2962
Mph1103I ATGCAT 1 cut(s) 1451
MroI TCCGGA 2 cut(s) 159, 1495
MroXI GAANNNNTTC 2 cut(s) 177, 1300
MscI TGGCCA 3 cut(s) 716, 1526, 2962
MslI CAYNNNNRTG 1 cut(s) 3021
Msp20I TGGCCA 3 cut(s) 716, 1526, 2962
MspCI CTTAAG 1 cut(s) 722
MspI CCGG 5 cut(s) 148, 160, 1496, 1693, 3102
MspR9I CCNGG 8 cut(s) 128, 148, 332, 1353, 1694, 2729, 2842, 3102
MunI CAATTG 2 cut(s) 819, 2459
Mva1269I GAATGC 3 cut(s) 76, 157, 1449
MvaI CCWGG 5 cut(s) 128, 332, 1353, 2729, 2842
MvnI CGCG 3 cut(s) 771, 1083, 2184
MwoI GCNNNNNNNGC 6 cut(s) 184, 2233, 2350, 2470, 2554, 2755
NciI CCSGG 3 cut(s) 148, 1694, 3102
NcoI CCATGG 1 cut(s) 2983
NdeI CATATG 1 cut(s) 1287
NlaIII CATG 8 cut(s) 97, 1279, 1449, 1673, 2969, 2987, 3020, 3026
NlaIV GGNNCC 3 cut(s) 1697, 1705, 1719
NmuCI GTSAC 1 cut(s) 1810
NruI TCGCGA 1 cut(s) 771
NsiI ATGCAT 1 cut(s) 1451
NspI RCATGY 1 cut(s) 1449
NspV TTCGAA 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 2111
PciSI GCTCTTC 2 cut(s) 168, 849
PcsI WCGNNNNNNNCGW 1 cut(s) 1413
PctI GAATGC 3 cut(s) 76, 157, 1449
PdmI GAANNNNTTC 2 cut(s) 177, 1300
PfeI GAWTC 7 cut(s) 140, 316, 421, 808, 1301, 1766, 2141
Pfl23II CGTACG 1 cut(s) 63
PflMI CCANNNNNTGG 2 cut(s) 114, 1533
PfoI TCCNGGA 2 cut(s) 126, 3100
PkrI GCNGC 5 cut(s) 619, 1269, 1915, 2003, 2862
PleI GAGTC 9 cut(s) 57, 76, 244, 496, 601, 823, 1051, 1348, 2122
PpsI GAGTC 9 cut(s) 57, 76, 244, 496, 601, 823, 1051, 1348, 2122
Ppu21I YACGTR 1 cut(s) 1657
PshBI ATTAAT 1 cut(s) 1491
PsiI TTATAA 1 cut(s) 1889
Psp124BI GAGCTC 2 cut(s) 906, 1641
Psp1406I AACGTT 2 cut(s) 869, 2818
Psp6I CCWGG 5 cut(s) 126, 330, 1351, 2727, 2840
PspGI CCWGG 5 cut(s) 126, 330, 1351, 2727, 2840
PspLI CGTACG 1 cut(s) 63
PspN4I GGNNCC 3 cut(s) 1697, 1705, 1719
PspOMI GGGCCC 1 cut(s) 1695
PspPI GGNCC 4 cut(s) 162, 987, 1695, 1696
PspXI VCTCGAGB 1 cut(s) 2111
PsrI GAACNNNNNNTAC 2 cut(s) 2448, 2480
PstI CTGCAG 2 cut(s) 2006, 2274
PstNI CAGNNNCTG 1 cut(s) 628
PsuI RGATCY 2 cut(s) 121, 1153
RruI TCGCGA 1 cut(s) 771
RsaI GTAC 8 cut(s) 65, 369, 433, 515, 1705, 1962, 2430, 2998
RsaNI GTAC 8 cut(s) 64, 368, 432, 514, 1704, 1961, 2429, 2997
RseI CAYNNNNRTG 1 cut(s) 3021
SacI GAGCTC 2 cut(s) 906, 1641
SapI GCTCTTC 2 cut(s) 168, 849
SatI GCNGC 5 cut(s) 618, 1268, 1914, 2002, 2861
Sau96I GGNCC 4 cut(s) 162, 987, 1695, 1696
SchI GAGTC 9 cut(s) 58, 77, 245, 497, 602, 824, 1052, 1349, 2123
ScrFI CCNGG 8 cut(s) 128, 148, 332, 1353, 1694, 2729, 2842, 3102
SduI GDGCHC 3 cut(s) 906, 1641, 1699
SexAI ACCWGGT 2 cut(s) 2727, 2840
SfaNI GCATC 7 cut(s) 23, 38, 615, 793, 1360, 1819, 2767
SfcI CTRYAG 3 cut(s) 2002, 2270, 2889
Sfr274I CTCGAG 1 cut(s) 2111
SfuI TTCGAA 1 cut(s) 171
SinI GGWCC 2 cut(s) 162, 987
SlaI CTCGAG 1 cut(s) 2111
SmiMI CAYNNNNRTG 1 cut(s) 3021
SmlI CTYRAG 7 cut(s) 722, 812, 1157, 1191, 1770, 2111, 2483
SmoI CTYRAG 7 cut(s) 722, 812, 1157, 1191, 1770, 2111, 2483
SsiI CCGC 3 cut(s) 617, 1083, 2783
SspMI CTAG 2 cut(s) 53, 2490
SstI GAGCTC 2 cut(s) 906, 1641
StyD4I CCNGG 8 cut(s) 126, 146, 330, 1351, 1692, 2727, 2840, 3100
StyI CCWWGG 8 cut(s) 207, 1699, 2322, 2364, 2601, 2685, 2772, 2983
TaaI ACNGT 3 cut(s) 518, 1405, 2991
TaiI ACGT 6 cut(s) 65, 346, 872, 1659, 2234, 2821
TaqII GACCGA 1 cut(s) 2920
TatI WGTACW 2 cut(s) 367, 1960
TauI GCSGC 1 cut(s) 620
TfiI GAWTC 7 cut(s) 140, 316, 421, 808, 1301, 1766, 2141
TscAI CASTG 2 cut(s) 1534, 1819
TseFI GTSAC 1 cut(s) 1810
TseI GCWGC 4 cut(s) 1267, 1913, 2001, 2860
Tsp45I GTSAC 1 cut(s) 1810
TspGWI ACGGA 2 cut(s) 260, 3048
TspRI CASTG 2 cut(s) 1534, 1819
Van91I CCANNNNNTGG 2 cut(s) 114, 1533
Vha464I CTTAAG 1 cut(s) 722
VpaK11BI GGWCC 2 cut(s) 162, 987
VspI ATTAAT 1 cut(s) 1491
XapI RAATTY 8 cut(s) 218, 839, 1058, 1096, 1261, 2249, 2941, 3054
XceI RCATGY 1 cut(s) 1449
XcmI CCANNNNNNNNNTGG 3 cut(s) 981, 2608, 2990
XhoI CTCGAG 1 cut(s) 2111
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 2 cut(s) 177, 1300
XspI CTAG 2 cut(s) 53, 2490
Zsp2I ATGCAT 1 cut(s) 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.